Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004355

Physical Location & Seq

Genomic Coordinates Forward (+)

6665826 .. 6666296

Gene Structure 471 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1094800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 471 bp

ATGCAGATCTTCGTGAAAACCCTAACGGGTAAGACCATAACCCTAGAGGTCGAGTCCTCCGACACCATCGACAATGTCAAGGCCAAGATCCAAGACAAGGAGGGCATCCCCCCGGACCAGCAGCGCCTCATCTTCGCCGGCAAGCAGCTCGAGGACGGCCGAACCCTCGCCGACTACAACATCCAGAAGGAGTCCACCCTCCACCTGGTGCTCCGCCTCCGCGGTGGCGCCAAGAAGAGGAAGAAGAAGACCTACACCAAGCCCAAGAAGATCAAGCACAAGCACAAGAAGGTGAAGCTCGCAGTGCTCCAGTTCTACAAGGTGGATGATTCCGGCAAGGTCCAGAGGCTGCGGAAGGAGTGCCCCAATGCCGAGTGCGGCGCTGGAACTTTCATGGCGAACCACTTCGACAGGCACTACTGCGGCAAGTGCGGATTGACCTATGTTTACCAGAAGGCTGGCGGTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03010 map03010

Pfam Domains

Protein Families

Ribosomal_S27 ubiquitin

Protein Analysis

157

Amino Acids

17.71

Weight (kDa)

9.77

Isoelectric Point (pI)

34.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad60-SLD	PF11976	1 - 71	1.5e-17	Ubiquitin-2 like Rad60 SUMO-like
ubiquitin	PF00240	3 - 74	1.9e-32	Ubiquitin family
Ribosomal_S27	PF01599	102 - 147	3.5e-26	Ribosomal protein S27a

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004355)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23410 AT2G47110 AT2G47110 AT3G62250
fragaria_vesca	FvH4_1g25981 FvH4_4g32881 FvH4_4g32881
malus_domestica	MD09G1293300.v1.1 MD13G1094800.v1.1 MD16G1095800.v1.1 MD17G1280900.v1.1
prunus_persica	Prupe.1G250600_v2.0.a1 Prupe.3G004600_v2.0.a1
pyrus_communis	pycom13g08260 pycom16g08190
rosa_chinensis	RchiOBHm_Chr3g0485301 RchiOBHm_Chr3g0485331 RchiOBHm_Chr3g0485341 RchiOBHm_Chr4g0441921
rosa_roxburghii	Rroxscaffold_6G00396770 Rroxscaffold_6G00397010
rosa_samantha	Rh3AG267000 Rh3AG267200 Rh3AG267300 Rh3CG301100 Rh4AG388700 Rh4CG417500
rosa_wichuraiana	Rw3G023720 Rw3G023790 Rw3G023830 Rw3G023890 Rw4G033570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	227
AccII	CGCG	1 cut(s)	222
AciI	CCGC	8 cut(s)	214, 220, 222, 352, 378, 423, 432, 462
AclWI	GGATC	1 cut(s)	82
AcoI	YGGCCR	1 cut(s)	157
AcyI	GRCGYC	1 cut(s)	228
AdeI	CACNNNGTG	1 cut(s)	208
AfiI	CCNNNNNNNGG	3 cut(s)	97, 205, 237
AjnI	CCWGG	1 cut(s)	204
AluBI	AGCT	2 cut(s)	148, 298
AluI	AGCT	2 cut(s)	148, 298
Alw21I	GWGCWC	2 cut(s)	213, 309
AlwI	GGATC	1 cut(s)	82
AlwNI	CAGNNNCTG	1 cut(s)	349
Ama87I	CYCGRG	1 cut(s)	149
AoxI	GGCC	2 cut(s)	81, 157
ApeKI	GCWGC	3 cut(s)	121, 145, 349
Asp700I	GAANNNNTTC	1 cut(s)	404
AspLEI	GCGC	3 cut(s)	126, 230, 383
AspS9I	GGNCC	2 cut(s)	115, 340
AsuC2I	CCSGG	1 cut(s)	113
AsuHPI	GGTGA	1 cut(s)	304
AvaI	CYCGRG	1 cut(s)	149
AvaII	GGWCC	2 cut(s)	115, 340
BaeGI	GKGCMC	1 cut(s)	365
BanI	GGYRCC	1 cut(s)	227
BarI	GAAGNNNNNNTAC	2 cut(s)	236, 268
BbsI	GAAGAC	1 cut(s)	254
Bbv12I	GWGCWC	2 cut(s)	213, 309
BbvI	GCAGC	3 cut(s)	133, 157, 336
BccI	CCATC	1 cut(s)	74
BceAI	ACGGC	1 cut(s)	172
BcgI	CGANNNNNNTGC	2 cut(s)	130, 164
BciT130I	CCWGG	1 cut(s)	206
BcnI	CCSGG	1 cut(s)	113
BfaI	CTAG	1 cut(s)	44
BfoI	RGCGCY	3 cut(s)	127, 231, 384
BglII	AGATCT	1 cut(s)	6
BisI	GCNGC	5 cut(s)	122, 146, 350, 379, 424
BlsI	GCNGC	5 cut(s)	123, 147, 351, 380, 425
Bme1390I	CCNGG	2 cut(s)	113, 206
Bme18I	GGWCC	2 cut(s)	115, 340
BmeT110I	CYCGRG	1 cut(s)	149
BmgT120I	GGNCC	2 cut(s)	115, 340
BmiI	GGNNCC	1 cut(s)	229
BmrFI	CCNGG	2 cut(s)	113, 206
BmsI	GCATC	1 cut(s)	114
BpiI	GAAGAC	1 cut(s)	254
BpmI	CTGGAG	1 cut(s)	293
BpuMI	CCSGG	1 cut(s)	113
BsaHI	GRCGYC	1 cut(s)	228
BsaJI	CCNNGG	2 cut(s)	111, 220
Bsc4I	CCNNNNNNNGG	3 cut(s)	97, 205, 237
Bse118I	RCCGGY	1 cut(s)	137
Bse1I	ACTGG	1 cut(s)	310
BseBI	CCWGG	1 cut(s)	206
BseDI	CCNNGG	2 cut(s)	111, 220
BseGI	GGATG	3 cut(s)	105, 180, 331
BseLI	CCNNNNNNNGG	3 cut(s)	97, 205, 237
BseNI	ACTGG	1 cut(s)	310
BseSI	GKGCMC	1 cut(s)	365
BseX3I	CGGCCG	1 cut(s)	157
BseXI	GCAGC	3 cut(s)	133, 157, 336
Bsh1236I	CGCG	1 cut(s)	222
Bsh1285I	CGRYCG	1 cut(s)	160
BshFI	GGCC	2 cut(s)	83, 159
BshNI	GGYRCC	1 cut(s)	227
BsiEI	CGRYCG	1 cut(s)	160
BsiHKAI	GWGCWC	2 cut(s)	213, 309
BsiHKCI	CYCGRG	1 cut(s)	149
BsiSI	CCGG	3 cut(s)	113, 138, 333
BslI	CCNNNNNNNGG	3 cut(s)	97, 205, 237
BsnI	GGCC	2 cut(s)	83, 159
BsoBI	CYCGRG	1 cut(s)	149
Bsp1286I	GDGCHC	3 cut(s)	213, 309, 365
Bsp143I	GATC	3 cut(s)	6, 87, 270
BspACI	CCGC	8 cut(s)	214, 220, 222, 352, 378, 423, 432, 462
BspANI	GGCC	2 cut(s)	83, 159
BspFNI	CGCG	1 cut(s)	222
BspLI	GGNNCC	1 cut(s)	229
BspPI	GGATC	1 cut(s)	82
BspT107I	GGYRCC	1 cut(s)	227
BsrFI	RCCGGY	1 cut(s)	137
BsrI	ACTGG	1 cut(s)	310
BssAI	RCCGGY	1 cut(s)	137
BssECI	CCNNGG	2 cut(s)	111, 220
BssMI	GATC	3 cut(s)	6, 87, 270
BssNI	GRCGYC	1 cut(s)	228
Bst2UI	CCWGG	1 cut(s)	206
Bst6I	CTCTTC	1 cut(s)	230
BstACI	GRCGYC	1 cut(s)	228
BstC8I	GCNNGC	4 cut(s)	139, 143, 300, 460
BstDSI	CCRYGG	1 cut(s)	220
BstF5I	GGATG	3 cut(s)	105, 180, 331
BstFNI	CGCG	1 cut(s)	222
BstH2I	RGCGCY	3 cut(s)	127, 231, 384
BstHHI	GCGC	3 cut(s)	126, 230, 383
BstKTI	GATC	3 cut(s)	9, 90, 273
BstMBI	GATC	3 cut(s)	6, 87, 270
BstMCI	CGRYCG	1 cut(s)	160
BstMWI	GCNNNNNNNGC	2 cut(s)	304, 429
BstNI	CCWGG	1 cut(s)	206
BstSCI	CCNGG	2 cut(s)	111, 204
BstSLI	GKGCMC	1 cut(s)	365
BstUI	CGCG	1 cut(s)	222
BstV1I	GCAGC	3 cut(s)	133, 157, 336
BstV2I	GAAGAC	1 cut(s)	254
BstX2I	RGATCY	2 cut(s)	6, 87
BstXI	CCANNNNNNTGG	1 cut(s)	458
BstYI	RGATCY	2 cut(s)	6, 87
BstZI	CGGCCG	1 cut(s)	157
BsuRI	GGCC	2 cut(s)	83, 159
BtgI	CCRYGG	1 cut(s)	220
BtsCI	GGATG	3 cut(s)	105, 180, 331
BtsI	GCAGTG	1 cut(s)	309
BtsIMutI	CAGTG	1 cut(s)	309
Cac8I	GCNNGC	4 cut(s)	139, 143, 300, 460
CaiI	CAGNNNCTG	1 cut(s)	349
CfoI	GCGC	3 cut(s)	126, 230, 383
Cfr10I	RCCGGY	1 cut(s)	137
Cfr13I	GGNCC	2 cut(s)	115, 340
Cfr42I	CCGCGG	1 cut(s)	223
CsiI	ACCWGGT	1 cut(s)	204
CviAII	CATG	1 cut(s)	394
CviJI	RGCY	7 cut(s)	83, 148, 159, 262, 298, 349, 458
CviKI_1	RGCY	7 cut(s)	83, 148, 159, 262, 298, 349, 458
DinI	GGCGCC	1 cut(s)	229
DpnI	GATC	3 cut(s)	8, 89, 272
DpnII	GATC	3 cut(s)	6, 87, 270
DraIII	CACNNNGTG	1 cut(s)	208
EaeI	YGGCCR	1 cut(s)	157
EagI	CGGCCG	1 cut(s)	157
Eam1104I	CTCTTC	1 cut(s)	230
EarI	CTCTTC	1 cut(s)	230
EciI	GGCGGA	1 cut(s)	203
EclXI	CGGCCG	1 cut(s)	157
Eco47I	GGWCC	2 cut(s)	115, 340
Eco52I	CGGCCG	1 cut(s)	157
Eco88I	CYCGRG	1 cut(s)	149
EcoRII	CCWGG	1 cut(s)	204
EgeI	GGCGCC	1 cut(s)	229
EheI	GGCGCC	1 cut(s)	229
FaeI	CATG	1 cut(s)	397
FaiI	YATR	3 cut(s)	38, 395, 444
FatI	CATG	1 cut(s)	393
Fnu4HI	GCNGC	5 cut(s)	122, 146, 350, 379, 424
FokI	GGATG	3 cut(s)	92, 167, 338
Fsp4HI	GCNGC	5 cut(s)	122, 146, 350, 379, 424
FspBI	CTAG	1 cut(s)	44
GlaI	GCGC	3 cut(s)	125, 229, 382
GluI	GCNGC	5 cut(s)	122, 146, 350, 379, 424
GsuI	CTGGAG	1 cut(s)	293
HaeII	RGCGCY	3 cut(s)	127, 231, 384
HaeIII	GGCC	2 cut(s)	83, 159
HapII	CCGG	3 cut(s)	113, 138, 333
HhaI	GCGC	3 cut(s)	126, 230, 383
Hin1I	GRCGYC	1 cut(s)	228
Hin1II	CATG	1 cut(s)	397
Hin6I	GCGC	3 cut(s)	124, 228, 381
HinP1I	GCGC	3 cut(s)	124, 228, 381
HinfI	GANTC	3 cut(s)	53, 191, 329
HpaII	CCGG	3 cut(s)	113, 138, 333
HphI	GGTGA	1 cut(s)	304
Hpy166II	GTNNAC	2 cut(s)	195, 448
Hpy188I	TCNGA	1 cut(s)	61
Hpy188III	TCNNGA	3 cut(s)	13, 184, 343
Hpy8I	GTNNAC	2 cut(s)	195, 448
HpyAV	CCTTC	4 cut(s)	181, 283, 349, 448
HpyCH4V	TGCA	1 cut(s)	4
HpyF10VI	GCNNNNNNNGC	2 cut(s)	304, 429
Hsp92I	GRCGYC	1 cut(s)	228
Hsp92II	CATG	1 cut(s)	397
HspAI	GCGC	3 cut(s)	124, 228, 381
KasI	GGCGCC	1 cut(s)	227
KroI	GCCGGC	1 cut(s)	137
KroNI	GCCGGC	1 cut(s)	139
KspI	CCGCGG	1 cut(s)	223
Kzo9I	GATC	3 cut(s)	6, 87, 270
LmnI	GCTCC	2 cut(s)	216, 312
Lsp1109I	GCAGC	3 cut(s)	133, 157, 336
LweI	GCATC	1 cut(s)	114
MabI	ACCWGGT	1 cut(s)	204
MaeI	CTAG	1 cut(s)	44
MalI	GATC	3 cut(s)	8, 89, 272
MboI	GATC	3 cut(s)	6, 87, 270
MboII	GAAGA	6 cut(s)	124, 247, 253, 256, 259, 280
MflI	RGATCY	2 cut(s)	6, 87
MhlI	GDGCHC	3 cut(s)	213, 309, 365
Mly113I	GGCGCC	1 cut(s)	228
MlyI	GAGTC	2 cut(s)	62, 200
MmeI	TCCRAC	1 cut(s)	84
MroNI	GCCGGC	1 cut(s)	137
MroXI	GAANNNNTTC	1 cut(s)	404
MspA1I	CMGCKG	1 cut(s)	222
MspI	CCGG	3 cut(s)	113, 138, 333
MspR9I	CCNGG	2 cut(s)	113, 206
MvaI	CCWGG	1 cut(s)	206
MvnI	CGCG	1 cut(s)	222
MwoI	GCNNNNNNNGC	2 cut(s)	304, 429
NaeI	GCCGGC	1 cut(s)	139
NarI	GGCGCC	1 cut(s)	228
NciI	CCSGG	1 cut(s)	113
NdeII	GATC	3 cut(s)	6, 87, 270
NgoMIV	GCCGGC	1 cut(s)	137
NlaIII	CATG	1 cut(s)	397
NlaIV	GGNNCC	1 cut(s)	229
NmeAIII	GCCGAG	1 cut(s)	397
PaeR7I	CTCGAG	1 cut(s)	149
PcsI	WCGNNNNNNNCGW	1 cut(s)	57
PdiI	GCCGGC	1 cut(s)	139
PdmI	GAANNNNTTC	1 cut(s)	404
PfeI	GAWTC	1 cut(s)	329
PflFI	GACNNNGTC	1 cut(s)	74
PkrI	GCNGC	5 cut(s)	123, 147, 351, 380, 425
PleI	GAGTC	2 cut(s)	61, 199
PluTI	GGCGCC	1 cut(s)	231
PpsI	GAGTC	2 cut(s)	61, 199
Psp6I	CCWGG	1 cut(s)	204
PspGI	CCWGG	1 cut(s)	204
PspN4I	GGNNCC	1 cut(s)	229
PspPI	GGNCC	2 cut(s)	115, 340
PspXI	VCTCGAGB	1 cut(s)	149
PstNI	CAGNNNCTG	1 cut(s)	349
PsuI	RGATCY	2 cut(s)	6, 87
PsyI	GACNNNGTC	1 cut(s)	74
SacII	CCGCGG	1 cut(s)	223
SatI	GCNGC	5 cut(s)	122, 146, 350, 379, 424
Sau3AI	GATC	3 cut(s)	6, 87, 270
Sau96I	GGNCC	2 cut(s)	115, 340
SchI	GAGTC	2 cut(s)	62, 200
ScrFI	CCNGG	2 cut(s)	113, 206
SduI	GDGCHC	3 cut(s)	213, 309, 365
SetI	ASST	9 cut(s)	51, 150, 207, 254, 294, 300, 324, 342, 443
SexAI	ACCWGGT	1 cut(s)	204
SfaNI	GCATC	1 cut(s)	114
SfoI	GGCGCC	1 cut(s)	229
Sfr274I	CTCGAG	1 cut(s)	149
Sfr303I	CCGCGG	1 cut(s)	223
SgrBI	CCGCGG	1 cut(s)	223
SinI	GGWCC	2 cut(s)	115, 340
SlaI	CTCGAG	1 cut(s)	149
SmlI	CTYRAG	1 cut(s)	149
SmoI	CTYRAG	1 cut(s)	149
SsiI	CCGC	8 cut(s)	214, 220, 222, 352, 378, 423, 432, 462
SspDI	GGCGCC	1 cut(s)	227
SspMI	CTAG	1 cut(s)	44
StyD4I	CCNGG	2 cut(s)	111, 204
TaqI	TCGA	4 cut(s)	51, 69, 150, 408
TauI	GCSGC	2 cut(s)	381, 426
TfiI	GAWTC	1 cut(s)	329
TscAI	CASTG	1 cut(s)	309
TseI	GCWGC	3 cut(s)	121, 145, 349
TspDTI	ATGAA	1 cut(s)	382
TspRI	CASTG	1 cut(s)	309
Tth111I	GACNNNGTC	1 cut(s)	74
VpaK11BI	GGWCC	2 cut(s)	115, 340
XcmI	CCANNNNNNNNNTGG	1 cut(s)	202
XhoI	CTCGAG	1 cut(s)	149
XmnI	GAANNNNTTC	1 cut(s)	404
XspI	CTAG	1 cut(s)	44

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.