MD13G1138800.v1.1

High mobility group B protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010334
Physical Location & Seq
Forward (+)
10695420 .. 10696958
1539 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1138800.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGGGCTACTACTGCAAGGGAATTCGTGACAAGGGTGGAACAATTAGTCAGAGGCTCTTCACTTCGAAGTCCAAGAATCAAGATTGTGTTATAGGTTCTGATTGTTCCAAGGTGATCAAATGTGAGGTGAGTTTTGATTCTTCCATGATCATTAATGCGGAGGATGCTGGTAATTTGGGATTGCATCAGTTAGAAGCTTGTATGGACAAGAATAGAAAACATGAAAGAAAGTTCACTTTCTCCAGATTTGGAAATGGTTGTTGTGTTGTTTGTTGTGTTGTCCTCGATCGTGACTACTGCAAGGGCATCCGGGATAAGGATGGTGGCTCAAAATTTAGGATATACACTGTCGGAGGAAAATCTCTGGATCTACATCTCCTTTTTGTTGAAGTCACATCTCGTGGTGGGCTTGACAAGGTGATTAGAGATCACAAATGGAAAGCAGTGATTGTGGCCTTCAAATTTCCAACGACAATTACTAGTGTTTCGTTTGTCTTACGGAAGTACTACTTGTCTTTGCTGTATCACTTTGAGCAGGCCTATTATCTCCATAAAGAAGTTTTTAGCACCCCAGTGCTTAAACCAGTAAGCGAGGACCTTTTCAATGGGTCAGCCACCGTGAAGGAAAGTGCAGAAGTGCAATTTGGCTCTTCAATCATGGGAACCATTGATGGGAAATTTGACGGTGGATATGTGGTTACTGTCAACTTGGGTTCTGATGAACTGAAAGGTGTCTTGTATCATGCTCCTACAGCTACATATGTTTCTCAGAGTTTTTTGGACATGCCAACTCGACGGAACCGAAAGAGATCCAGGTTGGCATTACGTGATCCCTCTCGACCCAAGTCCAAAAGGAGCAGTTACAATTTCTTCTTTGCTGAGCATTATGCTAGAATCGCCAATGTTCTGAATGCTCCATTTAGTTCAAGTTCCATAAAGTTCGAGTACTTTGTGTCAAACGTTCTGACAGCAAAGAGTGATGTCTACGGAGTTGGAATGGTGCTATTGGAGCTTTTGACAGGGAAGAGGGCTCTGTTTAGAAATAGTGAAGACGGAGGTCCAATGGTGGTTGTGGAATACGCAGGGCAGCGAATAGTGGCAGGGAAGCTGCAAGGCATGTTGGATCAAAGGGTGGGGAAGCCGGACCCGAAGCAAGGCGAGGCTGTTGAGCTAGTGGCTTATACTGCTATGCATTATGCTAAACAGAGGAAGTTGGATTTCTGTTTTAAGGGGGGAGTATTGTTAGGATGCCAGATGGCATCATCTTGTGGTGACAGTTAG

Protein Analysis

427

Amino Acids

47.39

Weight (kDa)

9.23

Isoelectric Point (pI)

35.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARID PF01388 110 - 178 1.9e-14 ARID/BRIGHT DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 984
AciI CCGC 1 cut(s) 158
AclI AACGTT 1 cut(s) 960
AclWI GGATC 4 cut(s) 375, 804, 824, 1131
AcsI RAATTY 4 cut(s) 21, 332, 461, 677
AfaI GTAC 2 cut(s) 506, 947
AfiI CCNNNNNNNGG 3 cut(s) 316, 672, 1154
AgsI TTSAA 5 cut(s) 389, 460, 604, 654, 927
AhlI ACTAGT 1 cut(s) 479
AjnI CCWGG 1 cut(s) 812
AjuI GAANNNNNNNTTGG 2 cut(s) 627, 659
AleI CACNNNNGTG 1 cut(s) 572
AluBI AGCT 5 cut(s) 197, 755, 1012, 1108, 1171
AluI AGCT 5 cut(s) 197, 755, 1012, 1108, 1171
AlwI GGATC 4 cut(s) 375, 804, 824, 1131
AoxI GGCC 2 cut(s) 453, 537
ApeKI GCWGC 2 cut(s) 1087, 1108
ApoI RAATTY 4 cut(s) 21, 332, 461, 677
AseI ATTAAT 1 cut(s) 153
AspS9I GGNCC 3 cut(s) 595, 1058, 1144
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 3 cut(s) 124, 139, 430
AsuII TTCGAA 1 cut(s) 65
AvaII GGWCC 3 cut(s) 595, 1058, 1144
BanII GRGCYC 1 cut(s) 1033
BauI CACGAG 1 cut(s) 399
BbsI GAAGAC 1 cut(s) 1056
BbvI GCAGC 2 cut(s) 1095, 1099
BccI CCATC 3 cut(s) 314, 665, 1249
BciT130I CCWGG 1 cut(s) 814
BclI TGATCA 2 cut(s) 114, 147
BcnI CCSGG 1 cut(s) 311
BcuI ACTAGT 1 cut(s) 479
BfaI CTAG 3 cut(s) 480, 891, 1172
BfmI CTRYAG 1 cut(s) 750
BisI GCNGC 2 cut(s) 1088, 1109
BlpI GCTNAGC 1 cut(s) 879
BlsI GCNGC 2 cut(s) 1089, 1110
BmcAI AGTACT 2 cut(s) 506, 947
Bme1390I CCNGG 2 cut(s) 311, 814
Bme18I GGWCC 3 cut(s) 595, 1058, 1144
BmgT120I GGNCC 3 cut(s) 595, 1058, 1144
BmiI GGNNCC 3 cut(s) 664, 800, 1146
BmrFI CCNGG 2 cut(s) 311, 814
BmrI ACTGGG 1 cut(s) 566
BmsI GCATC 5 cut(s) 154, 193, 315, 1238, 1268
BmuI ACTGGG 1 cut(s) 566
BoxI GACNNNNGTC 2 cut(s) 844, 1056
BpiI GAAGAC 1 cut(s) 1056
BpmI CTGGAG 1 cut(s) 226
Bpu1102I GCTNAGC 1 cut(s) 879
Bpu14I TTCGAA 1 cut(s) 65
BpuMI CCSGG 1 cut(s) 311
BsaAI YACGTR 1 cut(s) 827
BsaBI GATNNNNATC 1 cut(s) 372
BsaJI CCNNGG 1 cut(s) 108
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 672, 1154
Bse1I ACTGG 2 cut(s) 572, 584
Bse8I GATNNNNATC 1 cut(s) 372
BseBI CCWGG 1 cut(s) 814
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 4 cut(s) 169, 306, 325, 1253
BseJI GATNNNNATC 1 cut(s) 372
BseLI CCNNNNNNNGG 3 cut(s) 316, 672, 1154
BseMII CTCAG 2 cut(s) 782, 870
BseNI ACTGG 2 cut(s) 572, 584
BseXI GCAGC 2 cut(s) 1095, 1099
BsgI GTGCAG 1 cut(s) 651
Bsh1285I CGRYCG 1 cut(s) 289
BshFI GGCC 2 cut(s) 455, 539
BsiEI CGRYCG 1 cut(s) 289
BsiSI CCGG 2 cut(s) 310, 1142
BslI CCNNNNNNNGG 3 cut(s) 316, 672, 1154
BsmI GAATGC 1 cut(s) 916
BsnI GGCC 2 cut(s) 455, 539
Bsp119I TTCGAA 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 1033
Bsp143I GATC 8 cut(s) 114, 147, 286, 367, 427, 809, 829, 1123
Bsp1720I GCTNAGC 1 cut(s) 879
BspACI CCGC 1 cut(s) 158
BspANI GGCC 2 cut(s) 455, 539
BspCNI CTCAG 2 cut(s) 781, 871
BspLI GGNNCC 3 cut(s) 664, 800, 1146
BspPI GGATC 4 cut(s) 375, 804, 824, 1131
BspQI GCTCTTC 2 cut(s) 62, 655
BspT104I TTCGAA 1 cut(s) 65
BsrI ACTGG 2 cut(s) 572, 584
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 8 cut(s) 114, 147, 286, 367, 427, 809, 829, 1123
BssSI CACGAG 1 cut(s) 399
BssT1I CCWWGG 1 cut(s) 108
Bst2BI CACGAG 1 cut(s) 399
Bst2UI CCWGG 1 cut(s) 814
Bst4CI ACNGT 5 cut(s) 349, 619, 686, 703, 1277
Bst6I CTCTTC 3 cut(s) 62, 655, 1019
BstBAI YACGTR 1 cut(s) 827
BstBI TTCGAA 1 cut(s) 65
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 2 cut(s) 768, 879
BstF5I GGATG 4 cut(s) 169, 306, 325, 1253
BstKTI GATC 8 cut(s) 117, 150, 289, 370, 430, 812, 832, 1126
BstMBI GATC 8 cut(s) 114, 147, 286, 367, 427, 809, 829, 1123
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 6 cut(s) 12, 164, 752, 896, 1009, 1184
BstNI CCWGG 1 cut(s) 814
BstNSI RCATGY 2 cut(s) 787, 1120
BstPAI GACNNNNGTC 2 cut(s) 844, 1056
BstSCI CCNGG 2 cut(s) 309, 812
BstSFI CTRYAG 1 cut(s) 750
BstV1I GCAGC 2 cut(s) 1095, 1099
BstV2I GAAGAC 1 cut(s) 1056
BstX2I RGATCY 2 cut(s) 367, 809
BstYI RGATCY 2 cut(s) 367, 809
BsuRI GGCC 2 cut(s) 455, 539
BtsCI GGATG 4 cut(s) 169, 306, 325, 1253
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 3 cut(s) 345, 450, 579
Cac8I GCNNGC 1 cut(s) 537
Cfr13I GGNCC 3 cut(s) 595, 1058, 1144
Csp6I GTAC 2 cut(s) 505, 946
CviAII CATG 6 cut(s) 145, 221, 658, 743, 784, 1117
CviQI GTAC 2 cut(s) 505, 946
DdeI CTNAG 2 cut(s) 768, 879
DpnI GATC 8 cut(s) 116, 149, 288, 369, 429, 811, 831, 1125
DpnII GATC 8 cut(s) 114, 147, 286, 367, 427, 809, 829, 1123
Eam1104I CTCTTC 3 cut(s) 62, 655, 1019
EarI CTCTTC 3 cut(s) 62, 655, 1019
Eco130I CCWWGG 1 cut(s) 108
Eco147I AGGCCT 1 cut(s) 539
Eco24I GRGCYC 1 cut(s) 1033
Eco47I GGWCC 3 cut(s) 595, 1058, 1144
EcoO109I RGGNCCY 1 cut(s) 595
EcoRI GAATTC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 812
EcoT14I CCWWGG 1 cut(s) 108
EcoT22I ATGCAT 1 cut(s) 1194
EcoT38I GRGCYC 1 cut(s) 1033
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 6 cut(s) 148, 224, 661, 746, 787, 1120
FalI AAGNNNNNCTT 2 cut(s) 494, 526
FatI CATG 6 cut(s) 144, 220, 657, 742, 783, 1116
FauNDI CATATG 1 cut(s) 760
FbaI TGATCA 2 cut(s) 114, 147
FblI GTMKAC 1 cut(s) 984
Fnu4HI GCNGC 2 cut(s) 1088, 1109
FokI GGATG 4 cut(s) 176, 293, 332, 1260
FriOI GRGCYC 1 cut(s) 1033
Fsp4HI GCNGC 2 cut(s) 1088, 1109
FspBI CTAG 3 cut(s) 480, 891, 1172
GluI GCNGC 2 cut(s) 1088, 1109
GsuI CTGGAG 1 cut(s) 226
HaeIII GGCC 2 cut(s) 455, 539
HapII CCGG 2 cut(s) 310, 1142
Hin1II CATG 6 cut(s) 148, 224, 661, 746, 787, 1120
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 3 cut(s) 76, 137, 894
HpaII CCGG 2 cut(s) 310, 1142
HphI GGTGA 3 cut(s) 124, 139, 430
Hpy166II GTNNAC 3 cut(s) 234, 706, 985
Hpy188I TCNGA 7 cut(s) 51, 100, 353, 718, 771, 909, 966
Hpy188III TCNNGA 6 cut(s) 26, 80, 243, 290, 365, 837
Hpy8I GTNNAC 3 cut(s) 234, 706, 985
Hpy99I CGWCG 1 cut(s) 798
HpyAV CCTTC 2 cut(s) 466, 616
HpyCH4III ACNGT 5 cut(s) 349, 619, 686, 703, 1277
HpyCH4IV ACGT 2 cut(s) 826, 960
HpyCH4V TGCA 7 cut(s) 15, 184, 300, 632, 640, 1111, 1192
HpyF10VI GCNNNNNNNGC 6 cut(s) 12, 164, 752, 896, 1009, 1184
HpyF3I CTNAG 2 cut(s) 768, 879
HpySE526I ACGT 2 cut(s) 826, 960
Hsp92II CATG 6 cut(s) 148, 224, 661, 746, 787, 1120
Ksp22I TGATCA 2 cut(s) 114, 147
Kzo9I GATC 8 cut(s) 114, 147, 286, 367, 427, 809, 829, 1123
LguI GCTCTTC 2 cut(s) 62, 655
LmnI GCTCC 4 cut(s) 751, 855, 919, 1009
Lsp1109I GCAGC 2 cut(s) 1095, 1099
LweI GCATC 5 cut(s) 154, 193, 315, 1238, 1268
MaeI CTAG 3 cut(s) 480, 891, 1172
MaeII ACGT 2 cut(s) 826, 960
MaeIII GTNAC 6 cut(s) 26, 290, 391, 697, 860, 1271
MalI GATC 8 cut(s) 116, 149, 288, 369, 429, 811, 831, 1125
MboI GATC 8 cut(s) 114, 147, 286, 367, 427, 809, 829, 1123
MboII GAAGA 6 cut(s) 49, 132, 642, 862, 1036, 1061
MflI RGATCY 2 cut(s) 367, 809
MhlI GDGCHC 1 cut(s) 1033
MluCI AATT 9 cut(s) 21, 42, 172, 332, 461, 474, 641, 677, 865
MmeI TCCRAC 5 cut(s) 331, 491, 973, 1101, 1194
Mph1103I ATGCAT 1 cut(s) 1194
MseI TTAA 3 cut(s) 153, 579, 1227
MslI CAYNNNNRTG 1 cut(s) 572
MspI CCGG 2 cut(s) 310, 1142
MspR9I CCNGG 2 cut(s) 311, 814
Mva1269I GAATGC 1 cut(s) 916
MvaI CCWGG 1 cut(s) 814
MwoI GCNNNNNNNGC 6 cut(s) 12, 164, 752, 896, 1009, 1184
NciI CCSGG 1 cut(s) 311
NdeI CATATG 1 cut(s) 760
NdeII GATC 8 cut(s) 114, 147, 286, 367, 427, 809, 829, 1123
NlaIII CATG 6 cut(s) 148, 224, 661, 746, 787, 1120
NlaIV GGNNCC 3 cut(s) 664, 800, 1146
NmuCI GTSAC 4 cut(s) 26, 290, 391, 1271
NsiI ATGCAT 1 cut(s) 1194
NspI RCATGY 2 cut(s) 787, 1120
NspV TTCGAA 1 cut(s) 65
OliI CACNNNNGTG 1 cut(s) 572
PceI AGGCCT 1 cut(s) 539
PciSI GCTCTTC 2 cut(s) 62, 655
PcsI WCGNNNNNNNCGW 1 cut(s) 799
PctI GAATGC 1 cut(s) 916
PfeI GAWTC 3 cut(s) 76, 137, 894
PfoI TCCNGGA 1 cut(s) 309
PkrI GCNGC 2 cut(s) 1089, 1110
Ple19I CGATCG 1 cut(s) 289
Ppu21I YACGTR 1 cut(s) 827
PpuMI RGGWCCY 1 cut(s) 595
PshAI GACNNNNGTC 2 cut(s) 844, 1056
PshBI ATTAAT 1 cut(s) 153
Psp1406I AACGTT 1 cut(s) 960
Psp5II RGGWCCY 1 cut(s) 595
Psp6I CCWGG 1 cut(s) 812
PspGI CCWGG 1 cut(s) 812
PspN4I GGNNCC 3 cut(s) 664, 800, 1146
PspPI GGNCC 3 cut(s) 595, 1058, 1144
PspPPI RGGWCCY 1 cut(s) 595
PsuI RGATCY 2 cut(s) 367, 809
PvuI CGATCG 1 cut(s) 289
RsaI GTAC 2 cut(s) 506, 947
RsaNI GTAC 2 cut(s) 505, 946
RseI CAYNNNNRTG 1 cut(s) 572
SapI GCTCTTC 2 cut(s) 62, 655
SaqAI TTAA 3 cut(s) 153, 579, 1227
SatI GCNGC 2 cut(s) 1088, 1109
Sau3AI GATC 8 cut(s) 114, 147, 286, 367, 427, 809, 829, 1123
Sau96I GGNCC 3 cut(s) 595, 1058, 1144
ScaI AGTACT 2 cut(s) 506, 947
ScrFI CCNGG 2 cut(s) 311, 814
SduI GDGCHC 1 cut(s) 1033
SfaNI GCATC 5 cut(s) 154, 193, 315, 1238, 1268
SfcI CTRYAG 1 cut(s) 750
SfuI TTCGAA 1 cut(s) 65
SinI GGWCC 3 cut(s) 595, 1058, 1144
SmiMI CAYNNNNRTG 1 cut(s) 572
SpeI ACTAGT 1 cut(s) 479
Sse9I AATT 9 cut(s) 21, 42, 172, 332, 461, 474, 641, 677, 865
SseBI AGGCCT 1 cut(s) 539
SsiI CCGC 1 cut(s) 158
SspMI CTAG 3 cut(s) 480, 891, 1172
StuI AGGCCT 1 cut(s) 539
StyD4I CCNGG 2 cut(s) 309, 812
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 5 cut(s) 349, 619, 686, 703, 1277
TaiI ACGT 2 cut(s) 829, 963
TaqI TCGA 5 cut(s) 65, 285, 793, 838, 942
TasI AATT 9 cut(s) 21, 42, 172, 332, 461, 474, 641, 677, 865
TatI WGTACW 2 cut(s) 504, 945
TfiI GAWTC 3 cut(s) 76, 137, 894
Tru1I TTAA 3 cut(s) 153, 579, 1227
Tru9I TTAA 3 cut(s) 153, 579, 1227
TscAI CASTG 3 cut(s) 352, 450, 579
TseFI GTSAC 4 cut(s) 26, 290, 391, 1271
TseI GCWGC 2 cut(s) 1087, 1108
Tsp45I GTSAC 4 cut(s) 26, 290, 391, 1271
TspDTI ATGAA 2 cut(s) 237, 735
TspGWI ACGGA 4 cut(s) 514, 811, 1002, 1068
TspRI CASTG 3 cut(s) 352, 450, 579
VpaK11BI GGWCC 3 cut(s) 595, 1058, 1144
VspI ATTAAT 1 cut(s) 153
XapI RAATTY 4 cut(s) 21, 332, 461, 677
XceI RCATGY 2 cut(s) 787, 1120
XmiI GTMKAC 1 cut(s) 984
XspI CTAG 3 cut(s) 480, 891, 1172
ZrmI AGTACT 2 cut(s) 506, 947
Zsp2I ATGCAT 1 cut(s) 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.