MD13G1167300.v1.1

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010192
Physical Location & Seq
Reverse (-)
13542764 .. 13545296
2533 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1167300.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGGAAGGTAATAAGGATGAAGCTTTGAGGTGTGTTCGAATTGCCGAAGAAGCAATTGCTTCCGGCAACAAAGGGCGTGCGTTGAAATTTATTAAGATAGCACAACGGCTTAATCAGAATTTGCAAGTTGATGCACTTTTGGTTAAGTGTGAGGCGATTAATTCGGGGTCTTCCGCATCTTCTGTTGATGAGAAGGGTGCTAGTGAGATTAGGAAGGGGGCCGGTGTGGAAAAGTTGGACCGCGGTTTGAATGGGGAGGTTAGTTATACCGAAGAGCATATTCAGTTGGTTAGGAAGATTAAGAGATACAAAGACTATTATGCAATTCTTGGCGTGGAGAAGACTTGCTCGGGTGAGGACATTAGGAAGGCTTATAGGAAATTGTCACTGAAAGTTCATCCTGATAAGAACAAGGCTCCTGGTTCAGAAGAGGCTTTTAAGATAGTGAGCAAGGCTTTCAAGTGTTTGAGTGATGGAGATTCGAGGAGGCAGTATGATCAGACTGGACTGGTTGATGAATTTGAGTACAACCAGCAGCACAATAATATGAGGAGGAGGAGGAGAGGTGGGAATGACTTTTTTGACGATGATTTTGACCCCGATGAGATATTCAGGGCGTTCTTTGGTCAATCAGACATGTTTCGGACAGGTCGTGTTTATAGGACTAGTAGACCAGCTGGCCATCAGAGGGAGGAGTCTCAGGGAGGGGGACCTAACATCATGCTTCTTGTTCAAATATTACCATTCTTGGTAATTGTGTTGTTAGCATATCTGCCCTTCTCTGAGCCTGTCTACTCGTTGCAGAAGAATTACAACTACCAGATTCCCAAGACGACAGAGAAATATGGAGTGGAATTTTTTGTTAAATCGCAAGCATTTGATGAGAGTTATCCTGTTGGAAGTGTTGCTCGAGATAACATAGAGAACAGCGTTATCAAGGATTACAAAAATGTGCTTGTACACTACTGTCGGGTTGAGCTTCAAAGGCGTCACTGGAGTAAGAATCTACCTACTCCTCACTGCGACAAATTGAATAAGCTTGGAAGGCTATACGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DUF1977 DnaJ

Protein Analysis

353

Amino Acids

40.26

Weight (kDa)

9.06

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 105 - 166 6.2e-24 DnaJ domain
DUF1977 PF09320 261 - 331 1.7e-07 Domain of unknown function (DUF1977)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 670, 792
AccII CGCG 1 cut(s) 243
AciI CCGC 3 cut(s) 174, 241, 243
AcoI YGGCCR 1 cut(s) 679
AcsI RAATTY 4 cut(s) 86, 118, 518, 854
AcyI GRCGYC 1 cut(s) 988
AfaI GTAC 2 cut(s) 527, 960
AfiI CCNNNNNNNGG 1 cut(s) 688
AflIII ACRYGT 1 cut(s) 636
AgsI TTSAA 6 cut(s) 85, 250, 460, 734, 983, 1033
AhlI ACTAGT 1 cut(s) 665
AjnI CCWGG 1 cut(s) 418
AluBI AGCT 4 cut(s) 23, 677, 979, 1039
AluI AGCT 4 cut(s) 23, 677, 979, 1039
Alw26I GTCTC 1 cut(s) 702
Ama87I CYCGRG 2 cut(s) 349, 909
AoxI GGCC 2 cut(s) 219, 679
ApeKI GCWGC 1 cut(s) 535
ApoI RAATTY 4 cut(s) 86, 118, 518, 854
ArsI GACNNNNNNTTYG 2 cut(s) 575, 607
AseI ATTAAT 1 cut(s) 159
AspS9I GGNCC 3 cut(s) 219, 238, 710
AsuHPI GGTGA 1 cut(s) 365
AsuII TTCGAA 1 cut(s) 37
AvaI CYCGRG 2 cut(s) 349, 909
AvaII GGWCC 2 cut(s) 238, 710
BalI TGGCCA 1 cut(s) 681
BbsI GAAGAC 2 cut(s) 162, 347
BbvI GCAGC 1 cut(s) 547
BccI CCATC 2 cut(s) 467, 690
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 1 cut(s) 420
BclI TGATCA 1 cut(s) 496
BcoDI GTCTC 1 cut(s) 702
BcuI ACTAGT 1 cut(s) 665
BfaI CTAG 2 cut(s) 201, 666
BisI GCNGC 1 cut(s) 536
BlsI GCNGC 1 cut(s) 537
Bme1390I CCNGG 1 cut(s) 420
Bme18I GGWCC 2 cut(s) 238, 710
BmeT110I CYCGRG 2 cut(s) 349, 909
BmgT120I GGNCC 3 cut(s) 219, 238, 710
BmiI GGNNCC 3 cut(s) 220, 417, 711
BmrFI CCNGG 1 cut(s) 420
BmsI GCATC 2 cut(s) 121, 185
BpiI GAAGAC 2 cut(s) 162, 347
BpmI CTGGAG 1 cut(s) 1015
Bpu14I TTCGAA 1 cut(s) 37
BsaHI GRCGYC 1 cut(s) 988
BsaJI CCNNGG 1 cut(s) 241
BsaXI ACNNNNNCTCC 2 cut(s) 550, 580
Bsc4I CCNNNNNNNGG 1 cut(s) 688
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 3 cut(s) 508, 513, 998
BseBI CCWGG 1 cut(s) 420
BseDI CCNNGG 1 cut(s) 241
BseGI GGATG 2 cut(s) 22, 397
BseLI CCNNNNNNNGG 1 cut(s) 688
BseMII CTCAG 2 cut(s) 713, 774
BseNI ACTGG 3 cut(s) 508, 513, 998
BseRI GAGGAG 7 cut(s) 499, 565, 568, 571, 574, 707, 1005
BseXI GCAGC 1 cut(s) 547
Bsh1236I CGCG 1 cut(s) 243
BshFI GGCC 2 cut(s) 221, 681
BsiHKCI CYCGRG 2 cut(s) 349, 909
BsiSI CCGG 2 cut(s) 63, 222
BslFI GGGAC 1 cut(s) 723
BslI CCNNNNNNNGG 1 cut(s) 688
BsmAI GTCTC 1 cut(s) 702
BsmFI GGGAC 1 cut(s) 723
BsnI GGCC 2 cut(s) 221, 681
BsoBI CYCGRG 2 cut(s) 349, 909
Bsp119I TTCGAA 1 cut(s) 37
Bsp1407I TGTACA 1 cut(s) 958
Bsp143I GATC 1 cut(s) 496
BspACI CCGC 3 cut(s) 174, 241, 243
BspANI GGCC 2 cut(s) 221, 681
BspCNI CTCAG 2 cut(s) 712, 775
BspFNI CGCG 1 cut(s) 243
BspLI GGNNCC 3 cut(s) 220, 417, 711
BspQI GCTCTTC 1 cut(s) 267
BspT104I TTCGAA 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 221
BsrGI TGTACA 1 cut(s) 958
BsrI ACTGG 3 cut(s) 508, 513, 998
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 1 cut(s) 496
BssNI GRCGYC 1 cut(s) 988
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 1 cut(s) 968
Bst6I CTCTTC 2 cut(s) 267, 423
BstACI GRCGYC 1 cut(s) 988
BstAUI TGTACA 1 cut(s) 958
BstBI TTCGAA 1 cut(s) 37
BstC8I GCNNGC 3 cut(s) 78, 679, 873
BstDEI CTNAG 2 cut(s) 699, 783
BstDSI CCRYGG 1 cut(s) 241
BstF5I GGATG 2 cut(s) 22, 397
BstFNI CGCG 1 cut(s) 243
BstKTI GATC 1 cut(s) 499
BstMAI GTCTC 1 cut(s) 702
BstMBI GATC 1 cut(s) 496
BstMWI GCNNNNNNNGC 3 cut(s) 50, 985, 1045
BstNI CCWGG 1 cut(s) 420
BstNSI RCATGY 1 cut(s) 640
BstSCI CCNGG 1 cut(s) 418
BstUI CGCG 1 cut(s) 243
BstV1I GCAGC 1 cut(s) 547
BstV2I GAAGAC 2 cut(s) 162, 347
BsuRI GGCC 2 cut(s) 221, 681
BtgI CCRYGG 1 cut(s) 241
BtsCI GGATG 2 cut(s) 22, 397
BtsI GCAGTG 1 cut(s) 1018
BtsIMutI CAGTG 3 cut(s) 386, 991, 1018
Cac8I GCNNGC 3 cut(s) 78, 679, 873
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 3 cut(s) 219, 238, 710
Cfr42I CCGCGG 1 cut(s) 244
CseI GACGC 1 cut(s) 977
Csp6I GTAC 2 cut(s) 526, 959
CviAII CATG 2 cut(s) 637, 721
CviQI GTAC 2 cut(s) 526, 959
DdeI CTNAG 2 cut(s) 699, 783
DpnI GATC 1 cut(s) 498
DpnII GATC 1 cut(s) 496
EaeI YGGCCR 1 cut(s) 679
Eam1104I CTCTTC 2 cut(s) 267, 423
EarI CTCTTC 2 cut(s) 267, 423
Eco47I GGWCC 2 cut(s) 238, 710
Eco88I CYCGRG 2 cut(s) 349, 909
EcoO109I RGGNCCY 1 cut(s) 710
EcoRII CCWGG 1 cut(s) 418
FaeI CATG 2 cut(s) 640, 724
FaqI GGGAC 1 cut(s) 723
FatI CATG 2 cut(s) 636, 720
FbaI TGATCA 1 cut(s) 496
FblI GTMKAC 2 cut(s) 670, 792
Fnu4HI GCNGC 1 cut(s) 536
FokI GGATG 2 cut(s) 29, 384
Fsp4HI GCNGC 1 cut(s) 536
FspBI CTAG 2 cut(s) 201, 666
GluI GCNGC 1 cut(s) 536
GsuI CTGGAG 1 cut(s) 1015
HaeIII GGCC 2 cut(s) 221, 681
HapII CCGG 2 cut(s) 63, 222
HgaI GACGC 1 cut(s) 977
Hin1I GRCGYC 1 cut(s) 988
Hin1II CATG 2 cut(s) 640, 724
HindIII AAGCTT 2 cut(s) 21, 1037
HinfI GANTC 4 cut(s) 479, 695, 823, 1003
HpaII CCGG 2 cut(s) 63, 222
HphI GGTGA 1 cut(s) 365
Hpy166II GTNNAC 3 cut(s) 671, 793, 961
Hpy188I TCNGA 7 cut(s) 117, 427, 501, 634, 645, 687, 784
Hpy188III TCNNGA 2 cut(s) 401, 911
Hpy8I GTNNAC 3 cut(s) 671, 793, 961
HpyAV CCTTC 5 cut(s) 187, 208, 361, 787, 1038
HpyCH4III ACNGT 1 cut(s) 968
HpyCH4V TGCA 4 cut(s) 124, 134, 323, 802
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 985, 1045
HpyF3I CTNAG 2 cut(s) 699, 783
Hsp92I GRCGYC 1 cut(s) 988
Hsp92II CATG 2 cut(s) 640, 724
Ksp22I TGATCA 1 cut(s) 496
KspI CCGCGG 1 cut(s) 244
Kzo9I GATC 1 cut(s) 496
LguI GCTCTTC 1 cut(s) 267
LmnI GCTCC 1 cut(s) 421
Lsp1109I GCAGC 1 cut(s) 547
LweI GCATC 2 cut(s) 121, 185
MaeI CTAG 2 cut(s) 201, 666
MaeIII GTNAC 2 cut(s) 384, 989
MalI GATC 1 cut(s) 498
MboI GATC 1 cut(s) 496
MboII GAAGA 8 cut(s) 59, 162, 171, 284, 307, 352, 440, 817
MfeI CAATTG 1 cut(s) 54
MlsI TGGCCA 1 cut(s) 681
MluNI TGGCCA 1 cut(s) 681
MlyI GAGTC 1 cut(s) 704
MmeI TCCRAC 2 cut(s) 216, 877
Mox20I TGGCCA 1 cut(s) 681
MscI TGGCCA 1 cut(s) 681
MseI TTAA 7 cut(s) 93, 111, 144, 159, 300, 438, 864
Msp20I TGGCCA 1 cut(s) 681
MspA1I CMGCKG 2 cut(s) 243, 677
MspI CCGG 2 cut(s) 63, 222
MspR9I CCNGG 1 cut(s) 420
MunI CAATTG 1 cut(s) 54
MvaI CCWGG 1 cut(s) 420
MvnI CGCG 1 cut(s) 243
MwoI GCNNNNNNNGC 3 cut(s) 50, 985, 1045
NdeII GATC 1 cut(s) 496
NlaIII CATG 2 cut(s) 640, 724
NlaIV GGNNCC 3 cut(s) 220, 417, 711
NmuCI GTSAC 2 cut(s) 384, 989
NspI RCATGY 1 cut(s) 640
NspV TTCGAA 1 cut(s) 37
PaeR7I CTCGAG 1 cut(s) 909
PciI ACATGT 1 cut(s) 636
PciSI GCTCTTC 1 cut(s) 267
PcsI WCGNNNNNNNCGW 1 cut(s) 649
PfeI GAWTC 3 cut(s) 479, 823, 1003
PkrI GCNGC 1 cut(s) 537
PleI GAGTC 1 cut(s) 703
PpsI GAGTC 1 cut(s) 703
PpuMI RGGWCCY 1 cut(s) 710
PscI ACATGT 1 cut(s) 636
PshBI ATTAAT 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 710
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 3 cut(s) 220, 417, 711
PspPI GGNCC 3 cut(s) 219, 238, 710
PspPPI RGGWCCY 1 cut(s) 710
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 2 cut(s) 527, 960
RsaNI GTAC 2 cut(s) 526, 959
SacII CCGCGG 1 cut(s) 244
SapI GCTCTTC 1 cut(s) 267
SaqAI TTAA 7 cut(s) 93, 111, 144, 159, 300, 438, 864
SatI GCNGC 1 cut(s) 536
Sau3AI GATC 1 cut(s) 496
Sau96I GGNCC 3 cut(s) 219, 238, 710
SchI GAGTC 1 cut(s) 704
ScrFI CCNGG 1 cut(s) 420
SfaNI GCATC 2 cut(s) 121, 185
Sfr274I CTCGAG 1 cut(s) 909
Sfr303I CCGCGG 1 cut(s) 244
SfuI TTCGAA 1 cut(s) 37
SgrBI CCGCGG 1 cut(s) 244
SinI GGWCC 2 cut(s) 238, 710
SlaI CTCGAG 1 cut(s) 909
SmlI CTYRAG 1 cut(s) 909
SmoI CTYRAG 1 cut(s) 909
SpeI ACTAGT 1 cut(s) 665
SsiI CCGC 3 cut(s) 174, 241, 243
SspI AATATT 1 cut(s) 738
SspMI CTAG 2 cut(s) 201, 666
StyD4I CCNGG 1 cut(s) 418
TaaI ACNGT 1 cut(s) 968
TaqI TCGA 3 cut(s) 37, 482, 910
TatI WGTACW 2 cut(s) 525, 958
TfiI GAWTC 3 cut(s) 479, 823, 1003
Tru1I TTAA 7 cut(s) 93, 111, 144, 159, 300, 438, 864
Tru9I TTAA 7 cut(s) 93, 111, 144, 159, 300, 438, 864
TscAI CASTG 3 cut(s) 393, 998, 1025
TseFI GTSAC 2 cut(s) 384, 989
TseI GCWGC 1 cut(s) 535
Tsp45I GTSAC 2 cut(s) 384, 989
TspDTI ATGAA 3 cut(s) 33, 386, 531
TspRI CASTG 3 cut(s) 393, 998, 1025
VpaK11BI GGWCC 2 cut(s) 238, 710
VspI ATTAAT 1 cut(s) 159
XapI RAATTY 4 cut(s) 86, 118, 518, 854
XceI RCATGY 1 cut(s) 640
XhoI CTCGAG 1 cut(s) 909
XmiI GTMKAC 2 cut(s) 670, 792
XspI CTAG 2 cut(s) 201, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.