MD13G1169900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012114
Physical Location & Seq
Reverse (-)
13768777 .. 13769776
1000 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1169900.v1.1.491

Sequence Viewer

Length: 444 bp
ATGGATCAGGCGCTTCGCCGAGCAGCTGGAAGAACGCGACCCACTTCGACCGTCGAGACGACGTCGTCAAAACCAAAGACCGTCGTCGCCGATCAGCGGCCTCCTCCGGTTCCCCCTGAACGGCCCGAGATCTCCAAGGTCGGCGGCGCTATTGATTCTGATAGCAATGCAGGAACAGGAGGAGCAGTCGAAAATGTGCTTGAAGAGCGAGATCCAAATTACGACGCCATGCTCAATCAAATGGTGGGTAGGATCAAATCAAAGCCCGGAGGCAAATTGGAAATGGGTGAGGCATTTGTGGTGGAAAAGTATAAGAGGCCTCTCCCGAAACTGAGGGATGGGAAGTCAGATTCCCGCAGTTATGAGGAGAGGCCTACGCCGCCGCTGGACTTTGAATGTAGCACAGCTGCGCCACATCATCCTGCTTCATCGGGGCAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0240

Protein Analysis

148

Amino Acids

15.82

Weight (kDa)

7.85

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012114)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G21400
fragaria_vesca FvH4_4g17860
malus_domestica MD13G1169900.v1.1 MD16G1171400.v1.1
prunus_persica Prupe.1G138200_v2.0.a1
pyrus_communis pycom13g14540 pycom16g14370
rosa_chinensis RchiOBHm_Chr4g0421971
rosa_laevigata RLG00000007649
rosa_multiflora Rmu_sc0003255.1_g000006 Rmu_sc0008440.1_g000014 Rmu_sc0012856.1_g000002
rosa_roxburghii Rroxscaffold_5G00364060
rosa_rugosa Rorug04G0176600
rosa_samantha Rh4AG235600 Rh4BG238900 Rh4CG250300 Rh4DG234400
rosa_wichuraiana Rw4G019910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 64
AatII GACGTC 1 cut(s) 65
AccII CGCG 1 cut(s) 37
AciI CCGC 5 cut(s) 97, 144, 355, 380, 383
AclWI GGATC 3 cut(s) 12, 206, 260
AcyI GRCGYC 2 cut(s) 62, 225
AfiI CCNNNNNNNGG 2 cut(s) 96, 120
AgsI TTSAA 2 cut(s) 203, 395
AluBI AGCT 2 cut(s) 26, 407
AluI AGCT 2 cut(s) 26, 407
Alw26I GTCTC 1 cut(s) 50
AlwI GGATC 3 cut(s) 12, 206, 260
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 4 cut(s) 98, 122, 317, 371
ApeKI GCWGC 2 cut(s) 23, 407
AspLEI GCGC 3 cut(s) 13, 149, 412
AspS9I GGNCC 1 cut(s) 123
AsuC2I CCSGG 1 cut(s) 267
AsuHPI GGTGA 1 cut(s) 299
AvaI CYCGRG 1 cut(s) 125
BbvI GCAGC 2 cut(s) 35, 394
BccI CCATC 1 cut(s) 332
BceAI ACGGC 1 cut(s) 137
BcnI CCSGG 1 cut(s) 267
BcoDI GTCTC 1 cut(s) 50
BfoI RGCGCY 2 cut(s) 14, 150
BglII AGATCT 1 cut(s) 129
BisI GCNGC 6 cut(s) 24, 98, 145, 380, 383, 408
BlsI GCNGC 6 cut(s) 25, 99, 146, 381, 384, 409
Bme1390I CCNGG 1 cut(s) 267
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 123
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 1 cut(s) 267
BoxI GACNNNNGTC 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 267
BsaHI GRCGYC 2 cut(s) 62, 225
BsaJI CCNNGG 1 cut(s) 135
BsaWI WCCGGW 1 cut(s) 106
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 120
Bse3DI GCAATG 1 cut(s) 172
BseDI CCNNGG 1 cut(s) 135
BseGI GGATG 2 cut(s) 343, 418
BseLI CCNNNNNNNGG 2 cut(s) 96, 120
BseMI GCAATG 1 cut(s) 172
BseMII CTCAG 1 cut(s) 323
BseRI GAGGAG 3 cut(s) 93, 195, 380
BseXI GCAGC 2 cut(s) 35, 394
Bsh1236I CGCG 1 cut(s) 37
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 4 cut(s) 100, 124, 319, 373
BsiEI CGRYCG 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 2 cut(s) 107, 267
BslI CCNNNNNNNGG 2 cut(s) 96, 120
BsmAI GTCTC 1 cut(s) 50
BsmBI CGTCTC 1 cut(s) 50
BsnI GGCC 4 cut(s) 100, 124, 319, 373
BsoBI CYCGRG 1 cut(s) 125
Bsp143I GATC 5 cut(s) 4, 91, 129, 211, 252
BspACI CCGC 5 cut(s) 97, 144, 355, 380, 383
BspANI GGCC 4 cut(s) 100, 124, 319, 373
BspCNI CTCAG 1 cut(s) 324
BspFNI CGCG 1 cut(s) 37
BspLI GGNNCC 1 cut(s) 111
BspPI GGATC 3 cut(s) 12, 206, 260
BspQI GCTCTTC 1 cut(s) 198
BsrDI GCAATG 1 cut(s) 172
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 5 cut(s) 4, 91, 129, 211, 252
BssNI GRCGYC 2 cut(s) 62, 225
BssT1I CCWWGG 1 cut(s) 135
Bst4CI ACNGT 2 cut(s) 52, 82
Bst6I CTCTTC 1 cut(s) 198
BstACI GRCGYC 2 cut(s) 62, 225
BstDEI CTNAG 1 cut(s) 332
BstF5I GGATG 2 cut(s) 343, 418
BstFNI CGCG 1 cut(s) 37
BstH2I RGCGCY 2 cut(s) 14, 150
BstHHI GCGC 3 cut(s) 13, 149, 412
BstKTI GATC 5 cut(s) 7, 94, 132, 214, 255
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 5 cut(s) 4, 91, 129, 211, 252
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 2 cut(s) 205, 379
BstPAI GACNNNNGTC 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 265
BstUI CGCG 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 35, 394
BstX2I RGATCY 2 cut(s) 129, 211
BstYI RGATCY 2 cut(s) 129, 211
BsuRI GGCC 4 cut(s) 100, 124, 319, 373
BtsCI GGATG 2 cut(s) 343, 418
CfoI GCGC 3 cut(s) 13, 149, 412
Cfr13I GGNCC 1 cut(s) 123
CseI GACGC 1 cut(s) 233
CviAII CATG 1 cut(s) 229
CviJI RGCY 8 cut(s) 26, 100, 124, 265, 319, 373, 407, 441
CviKI_1 RGCY 8 cut(s) 26, 100, 124, 265, 319, 373, 407, 441
DdeI CTNAG 1 cut(s) 332
DpnI GATC 5 cut(s) 6, 93, 131, 213, 254
DpnII GATC 5 cut(s) 4, 91, 129, 211, 252
DrdI GACNNNNNNGTC 1 cut(s) 64
DseDI GACNNNNNNGTC 1 cut(s) 64
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco130I CCWWGG 1 cut(s) 135
Eco147I AGGCCT 2 cut(s) 319, 373
Eco88I CYCGRG 1 cut(s) 125
EcoT14I CCWWGG 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 135
Esp3I CGTCTC 1 cut(s) 50
FaeI CATG 1 cut(s) 232
FaiI YATR 3 cut(s) 230, 312, 363
FatI CATG 1 cut(s) 228
FauI CCCGC 1 cut(s) 362
Fnu4HI GCNGC 6 cut(s) 24, 98, 145, 380, 383, 408
FokI GGATG 2 cut(s) 350, 405
Fsp4HI GCNGC 6 cut(s) 24, 98, 145, 380, 383, 408
GlaI GCGC 3 cut(s) 12, 148, 411
GluI GCNGC 6 cut(s) 24, 98, 145, 380, 383, 408
HaeII RGCGCY 2 cut(s) 14, 150
HaeIII GGCC 4 cut(s) 100, 124, 319, 373
HapII CCGG 2 cut(s) 107, 267
HgaI GACGC 1 cut(s) 233
HhaI GCGC 3 cut(s) 13, 149, 412
Hin1I GRCGYC 2 cut(s) 62, 225
Hin1II CATG 1 cut(s) 232
Hin6I GCGC 3 cut(s) 11, 147, 410
HinP1I GCGC 3 cut(s) 11, 147, 410
HinfI GANTC 2 cut(s) 155, 350
HpaII CCGG 2 cut(s) 107, 267
HphI GGTGA 1 cut(s) 299
Hpy188I TCNGA 2 cut(s) 160, 349
Hpy188III TCNNGA 2 cut(s) 55, 325
Hpy99I CGWCG 6 cut(s) 56, 64, 67, 86, 89, 227
HpyCH4III ACNGT 2 cut(s) 52, 82
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 1 cut(s) 170
HpyF10VI GCNNNNNNNGC 2 cut(s) 205, 379
HpyF3I CTNAG 1 cut(s) 332
HpySE526I ACGT 1 cut(s) 62
Hsp92I GRCGYC 2 cut(s) 62, 225
Hsp92II CATG 1 cut(s) 232
HspAI GCGC 3 cut(s) 11, 147, 410
Kzo9I GATC 5 cut(s) 4, 91, 129, 211, 252
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 1 cut(s) 182
LpnPI CCDG 8 cut(s) 12, 120, 129, 156, 162, 280, 371, 435
Lsp1109I GCAGC 2 cut(s) 35, 394
MaeII ACGT 1 cut(s) 62
MalI GATC 5 cut(s) 6, 93, 131, 213, 254
MboI GATC 5 cut(s) 4, 91, 129, 211, 252
MboII GAAGA 2 cut(s) 42, 215
MflI RGATCY 2 cut(s) 129, 211
MluCI AATT 2 cut(s) 217, 275
MspA1I CMGCKG 4 cut(s) 26, 97, 385, 407
MspI CCGG 2 cut(s) 107, 267
MspR9I CCNGG 1 cut(s) 267
MvnI CGCG 1 cut(s) 37
MwoI GCNNNNNNNGC 2 cut(s) 205, 379
NciI CCSGG 1 cut(s) 267
NdeII GATC 5 cut(s) 4, 91, 129, 211, 252
NlaIII CATG 1 cut(s) 232
NlaIV GGNNCC 1 cut(s) 111
NmeAIII GCCGAG 1 cut(s) 44
PceI AGGCCT 2 cut(s) 319, 373
PciSI GCTCTTC 1 cut(s) 198
PfeI GAWTC 2 cut(s) 155, 350
PflFI GACNNNGTC 2 cut(s) 61, 64
PkrI GCNGC 6 cut(s) 25, 99, 146, 381, 384, 409
PshAI GACNNNNGTC 1 cut(s) 83
PspN4I GGNNCC 1 cut(s) 111
PspPI GGNCC 1 cut(s) 123
PsuI RGATCY 2 cut(s) 129, 211
PsyI GACNNNGTC 2 cut(s) 61, 64
PvuII CAGCTG 2 cut(s) 26, 407
SapI GCTCTTC 1 cut(s) 198
SatI GCNGC 6 cut(s) 24, 98, 145, 380, 383, 408
Sau3AI GATC 5 cut(s) 4, 91, 129, 211, 252
Sau96I GGNCC 1 cut(s) 123
ScrFI CCNGG 1 cut(s) 267
SetI ASST 4 cut(s) 28, 65, 141, 409
Sse9I AATT 2 cut(s) 217, 275
SseBI AGGCCT 2 cut(s) 319, 373
SsiI CCGC 5 cut(s) 97, 144, 355, 380, 383
StuI AGGCCT 2 cut(s) 319, 373
StyD4I CCNGG 1 cut(s) 265
StyI CCWWGG 1 cut(s) 135
TaaI ACNGT 2 cut(s) 52, 82
TaiI ACGT 1 cut(s) 65
TaqI TCGA 3 cut(s) 47, 54, 189
TasI AATT 2 cut(s) 217, 275
TauI GCSGC 4 cut(s) 100, 147, 382, 385
TfiI GAWTC 2 cut(s) 155, 350
TseI GCWGC 2 cut(s) 23, 407
TspDTI ATGAA 1 cut(s) 417
Tth111I GACNNNGTC 2 cut(s) 61, 64
ZraI GACGTC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.