MD13G1201100.v1.1

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007173
Physical Location & Seq
Forward (+)
17940862 .. 17948419
7558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1201100.v1.1.491

Sequence Viewer

Length: 3399 bp
ATGCAAGTCCTCAATTCTGACGAAGCTGCCCCCGAAAACCCTAAGGAACCAGAGCAATCAGAAGCTGAAGCCTCCAATCCGACTGTCAAAGAGGAAGATGGCGGAGCCGCTGCCGATGGCGATGACGACGACTCGAGGGTTGTCGATATTTCCGGCAAAAGCTTGGACTTTTCGATCAGGGAGAATTCTGAGGATGCCGGGGCTTTGTATTTGTATAAAAACGTGTTTAATTTATTGCCGAAGTCGATTACCTCTCACAAGCGGCTGAGAACGCTGAAGTTCTTTGGGAATGAGATTAATTTATTTTCGCCGTCCGCGGCGATGGAGTTTGAGAGCTTGGAATGCTTGCAAGTGAGGATGTCGTCGCCGGAGTTTGGTGGGTTGCCGTTGGATAAATTGAGTGGCTTGAAGGAGCTTGAGCTTTCCAAAGTGCCGCCGAGGCCTTCGGGGTTTCAGATATTGAGCGAGATTGCTCGGCTCAAGAGCCTCACCAAGCTCTCTGTTTGTCACTTCTCTATAAGATACCTTCCTCCAGAAATTGGCTGCTTAAACACTTTGGAATATCTGGATCTTTCATTCAACAAGATAAAAAGTTTGCCTGCTGAGATCAGTAATTTAAATGCCTTGATATCATTAAAAGTTGCTAATAATAAATTGGTGGAATTGCCTTCGACTTTGTCCTCTTTGCAGAGGTTGGAAACAATGGACCTGTCCAATAATAGATTGACATCACTGGGATATCTTGAACTTGACTTGATGCACAACCTCCAGATTTTAAATCTTCAGTACAACAAGCTTCTCACTTTCTGTCAAATACCCTCATGGATACGCTGCAATTTAGAAGGAAATGGGAAGGACACACTCGGTGATGATTCTACCAGTTCCTCTGTAGAAATGGAAGTATATGAAACCCCCCTTGGGAACAATAATGAAAGTCTATTTCGCAGAGGCTTTCATCATAGCTCAGCAAGTTTAATTTTCGCACATTCAACGAACAGTAGGTGCTGTGCAACTCGGAGGTCAGGTAGGTGGAGGAAGCAAGGGTACAGTTTGCAGCAGAGAGCTCGTCAAGAACGCTTGAACAACAGTAGAAAGTTGAAAGGTCTTGATCCTTCCAAGCGGTTACACGTGCAGGAAGATGAAGAAAGCAAACCAGGCAACCAAGATCTTCTTGCTTCTGAATCTTATCCTGAAGGTGCATCAGACATTATCAATCTGGACGATTATGGTGATCAAGATGTACTATCTAGGGAAGTGCAAAGTGAAAATATTGTGTGCCATGATGTGAGCTCAAAGGAGCGTTTTGTAGGAAATTGCTCATCTGTGAGTCCAGACTCTAGCACAGTAGATGAAAGTGATGAAAAGGATTGTTGTGAGTTTGATGCATCGTCGGCCTCCAATCAACAGGTTTATGGTGAGGAAGATGAAGGTTCATCTTCAGATACATCAAAAAGTATTTGTCAACCCAAAAGGCACCCTGATGGGAACCTTGATAATCCGAGACGGCCCAAACATCCTAGATACTGTCCTGATAGTTCAAACTTGTCTCGGAAATATAATGACTTCTCGGTTTGCAGCACTGAGGACCATCTTTCTGATGGCTTTTATGATGCAGGGCGTGACAGGCCATTCATGCCACTAGAACTTTATGAGGAAAAATTCCACCTTGACTCACGTGAAGTCATTCTTGTGGACAGGCAATGGGATAAAGAGTTGGATGTTATTTTATGTTCTGCCCAAGAATTGGTGAATCGTTTAAATACAGATGGAAATAAGGCTGATATGCTGCAGATTGCATCATTGCTTGCTCTGTTTGTATCAGATCACTTTGGGGGAAGTGATAGGAGTGCTCTTGTTGAATGGGCACGAAAAGCTAATCCTCTTTCAGACTACAGGAAGCCTTTTGTTTGCACCTGCCCAACTGGAAACAAGGGCTTTATTAGTTTGTCCTCTAAACCAGTTTTAAAGAATGTTGAAGATATCGTTTTTTCTGATCTCTGTGAGAAATCCCTTCGTTCTATTAAAGCACGACGAAAATCCATCGTAGTTCCCATTGGAACCTTGCAGTTTGGTGTGTGTCGGCATAGAGCATTGCTATTGAAGTTTGGTGTGTGTCGGCATAGAGCATTGCTATTGAAGTATCTATGTGATTGGGTGGAGCCTCGGGTACCTTGTGAGCTTGTTAGGGGTTACATGGATTTCATGCCACATGCGTGGAATATTGTATCAATCAAGAGAGGTGCCTTAGAGATTCGAATGGTGGTGGATGCATGTCGTCCGCGTGATATAAGAGTGGAGACTGATCCTGAATATTATTGCAGGTATATTCCTCTCAGCCGGACCAAAGTTTATCTTCCAATTCGAAGCAGCCCTGCTCCTACCTCCTTCCCTTCTATGCCCTCTTGTGATGAAACTCTAAAAAACTCCGTTACTTCTCTTGTCCGGCACAAATATGGATCAAATGAGGCAGCAGGAAAGATGCGTACTTCCGTGGTATATGGAAATTCAACGGATGAAATTAGGAACTTTGACTACAACTGTTTAGGGGAAATAAGGATTTTAGGTGCTTTGAAACACCCTTGCATAGTGGAAATGTATGGACATCAGATATCTTCCAAGTGGGTTCCTTCTGGGGATGGTAGTCCTGAGCATCGTATATTGCAGTCTATAATTTGGATGGAGGAAATAAAAGGGGGTTCCTTACAGAATTATATGGAAAAGCTATCAAAAGCTGGTGAGAAGCATCTTCCAGTGGAGTTAGCTTTGTGCATCGCTAAAAATGTTGCATGTGCATTGGTGGAGCTGCATTCAAAGCACATTATTCACCGGGATGTAAAAAGTGCAAACATTTTGATTGATCTGGATAGGAAGAGAGCTGATGGAACACATGTTGTGAAGCTCTGTGATTTTGATAGGGCAGTTCCCCTTCGATCTTACTTGCATACATGCTGCATCGCCCACATAGGAATACCTCCTACTAATGTTTGTGCTGGAACACCTCGCTGGATGGCCCCAGAGGTTTTGCGAGCAGTGCATGAACGAAATATATATGGATTGGAAATTGATATCTGGTCGTTTGGGTGCTTGCTGTTGGAAATGTTGACTCTGCAAATTCCGTACTTCGGAGTACCTGAATTGGAGATTCATGAACTTCTGACGAGGGGAAAACGACCAAAGTTAACAGATGACCTGGAGGCGTTTCGGTCGTTGAATGAGCCAGTAATGACTCAAGCAGGCGCGGAACTTGAGGGAACAGAGTCTGACTTAGACACACTGAGATTTCTTGTTGACTTGTTCTACCAATGTACGGAGGAGAATCCGGAGAATCGTCCAACGGCTGACAACCTCTACGAATTGATACTGGAACACAGCAAAACTCTTACAAACTCGGAAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

1133

Amino Acids

127.42

Weight (kDa)

5.6

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 154 - 264 2.9e-11 Leucine-rich repeat region
LRR_8 PF13855 162 - 219 8.5e-06 Leucine rich repeat
EDR1_CTR1_ARMC3_pept PF14381 667 - 701 1e-05 EDR1/CTR1/ARMC3, peptidase-like
EDR1_CTR1_ARMC3_pept PF14381 700 - 756 2.8e-06 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 842 - 1117 2.6e-33 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 846 - 1061 2.8e-23 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1922
Acc36I ACCTGC 2 cut(s) 1922, 2310
Acc65I GGTACC 1 cut(s) 2167
AccB1I GGYRCC 3 cut(s) 1473, 2167, 2240
AccB7I CCANNNNNTGG 2 cut(s) 539, 1744
AccII CGCG 3 cut(s) 317, 2281, 3239
AccIII TCCGGA 1 cut(s) 3319
AciI CCGC 9 cut(s) 102, 108, 262, 315, 317, 434, 1120, 2279, 3239
AclWI GGATC 4 cut(s) 576, 1103, 2297, 2464
AcsI RAATTY 4 cut(s) 184, 1658, 2503, 3111
AcuI CTGAAG 5 cut(s) 87, 296, 767, 1212, 1422
AcvI CACGTG 2 cut(s) 1129, 1676
AdeI CACNNNGTG 1 cut(s) 866
AfaI GTAC 8 cut(s) 788, 1046, 1242, 2169, 2485, 3119, 3129, 3307
AfiI CCNNNNNNNGG 6 cut(s) 374, 539, 918, 1744, 3122, 3307
AflIII ACRYGT 3 cut(s) 222, 1126, 2887
AjnI CCWGG 2 cut(s) 1153, 3189
AjuI GAANNNNNNNTTGG 2 cut(s) 900, 932
AleI CACNNNNGTG 1 cut(s) 2212
Alw21I GWGCWC 3 cut(s) 1066, 1292, 1852
Alw26I GTCTC 3 cut(s) 1495, 1551, 2291
AlwI GGATC 4 cut(s) 576, 1103, 2297, 2464
AlwNI CAGNNNCTG 2 cut(s) 65, 3260
Ama87I CYCGRG 2 cut(s) 133, 2163
Aor13HI TCCGGA 1 cut(s) 3319
AoxI GGCC 5 cut(s) 440, 1392, 1505, 1625, 3009
ApoI RAATTY 4 cut(s) 184, 1658, 2503, 3111
AseI ATTAAT 1 cut(s) 297
Asp700I GAANNNNTTC 1 cut(s) 1683
Asp718I GGTACC 1 cut(s) 2167
AspLEI GCGC 1 cut(s) 3239
AspS9I GGNCC 5 cut(s) 706, 1506, 1585, 2340, 3010
AsuC2I CCSGG 2 cut(s) 199, 2828
AsuHPI GGTGA 7 cut(s) 481, 878, 1241, 1427, 1759, 2747, 2816
AsuII TTCGAA 2 cut(s) 2254, 2362
AvaI CYCGRG 2 cut(s) 133, 2163
AvaII GGWCC 3 cut(s) 706, 1585, 2340
AxyI CCTNAGG 1 cut(s) 42
BaeGI GKGCMC 1 cut(s) 1867
BanI GGYRCC 3 cut(s) 1473, 2167, 2240
BanII GRGCYC 2 cut(s) 1066, 1292
BarI GAAGNNNNNNTAC 2 cut(s) 1028, 1060
BbrPI CACGTG 2 cut(s) 1129, 1676
Bbv12I GWGCWC 3 cut(s) 1066, 1292, 1852
BceAI ACGGC 4 cut(s) 295, 370, 1520, 3351
BcgI CGANNNNNNTGC 2 cut(s) 1046, 1080
BciT130I CCWGG 2 cut(s) 1155, 3191
BciVI GTATCC 1 cut(s) 819
BclI TGATCA 1 cut(s) 1231
BcnI CCSGG 2 cut(s) 199, 2828
BcoDI GTCTC 3 cut(s) 1495, 1551, 2291
BfaI CTAG 4 cut(s) 1248, 1338, 1518, 1640
BfmI CTRYAG 3 cut(s) 888, 1787, 1891
BfuAI ACCTGC 2 cut(s) 1922, 2310
BfuI GTATCC 1 cut(s) 819
BglI GCCNNNNNGGC 1 cut(s) 439
BglII AGATCT 1 cut(s) 1165
BlpI GCTNAGC 1 cut(s) 964
Bme1390I CCNGG 4 cut(s) 199, 1155, 2828, 3191
Bme18I GGWCC 3 cut(s) 706, 1585, 2340
BmeT110I CYCGRG 2 cut(s) 133, 2163
BmgT120I GGNCC 5 cut(s) 706, 1506, 1585, 2340, 3010
BmrFI CCNGG 4 cut(s) 199, 1155, 2828, 3191
BmrI ACTGGG 1 cut(s) 743
BmuI ACTGGG 1 cut(s) 743
BpmI CTGGAG 3 cut(s) 516, 752, 3212
Bpu10I CCTNAGC 1 cut(s) 2646
Bpu1102I GCTNAGC 1 cut(s) 964
Bpu14I TTCGAA 2 cut(s) 2254, 2362
BpuEI CTTGAG 4 cut(s) 437, 464, 3213, 3266
BpuMI CCSGG 2 cut(s) 199, 2828
BsaAI YACGTR 2 cut(s) 1129, 1676
BsaBI GATNNNNATC 1 cut(s) 727
BsaJI CCNNGG 6 cut(s) 198, 315, 437, 916, 2162, 2490
BsaWI WCCGGW 1 cut(s) 3319
BsaXI ACNNNNNCTCC 2 cut(s) 1009, 1039
Bsc4I CCNNNNNNNGG 6 cut(s) 374, 539, 918, 1744, 3122, 3307
Bse1I ACTGG 7 cut(s) 738, 879, 1927, 1958, 2750, 3218, 3366
Bse21I CCTNAGG 1 cut(s) 42
Bse3DI GCAATG 4 cut(s) 1706, 1799, 2090, 2126
Bse8I GATNNNNATC 1 cut(s) 727
BseAI TCCGGA 1 cut(s) 3319
BseBI CCWGG 2 cut(s) 1155, 3191
BseDI CCNNGG 6 cut(s) 198, 315, 437, 916, 2162, 2490
BseJI GATNNNNATC 1 cut(s) 727
BseLI CCNNNNNNNGG 6 cut(s) 374, 539, 918, 1744, 3122, 3307
BseMI GCAATG 4 cut(s) 1706, 1799, 2090, 2126
BseMII CTCAG 8 cut(s) 180, 257, 594, 978, 1572, 2347, 2637, 3266
BseNI ACTGG 7 cut(s) 738, 879, 1927, 1958, 2750, 3218, 3366
BseRI GAGGAG 1 cut(s) 3326
BseSI GKGCMC 1 cut(s) 1867
BsgI GTGCAG 1 cut(s) 1151
Bsh1236I CGCG 3 cut(s) 317, 2281, 3239
Bsh1285I CGRYCG 1 cut(s) 3206
BshFI GGCC 5 cut(s) 442, 1394, 1507, 1627, 3011
BshNI GGYRCC 3 cut(s) 1473, 2167, 2240
BsiEI CGRYCG 1 cut(s) 3206
BsiHKAI GWGCWC 3 cut(s) 1066, 1292, 1852
BsiHKCI CYCGRG 2 cut(s) 133, 2163
BsiSI CCGG 7 cut(s) 153, 198, 368, 2338, 2443, 2827, 3320
BslI CCNNNNNNNGG 6 cut(s) 374, 539, 918, 1744, 3122, 3307
BsmAI GTCTC 3 cut(s) 1495, 1551, 2291
BsmBI CGTCTC 1 cut(s) 1495
BsmI GAATGC 2 cut(s) 347, 2806
BsnI GGCC 5 cut(s) 442, 1394, 1507, 1627, 3011
BsoBI CYCGRG 2 cut(s) 133, 2163
Bsp119I TTCGAA 2 cut(s) 2254, 2362
Bsp1286I GDGCHC 4 cut(s) 1066, 1292, 1852, 1867
Bsp13I TCCGGA 1 cut(s) 3319
Bsp1720I GCTNAGC 1 cut(s) 964
BspACI CCGC 9 cut(s) 102, 108, 262, 315, 317, 434, 1120, 2279, 3239
BspANI GGCC 5 cut(s) 442, 1394, 1507, 1627, 3011
BspCNI CTCAG 8 cut(s) 181, 258, 595, 977, 1573, 2346, 2638, 3267
BspEI TCCGGA 1 cut(s) 3319
BspFNI CGCG 3 cut(s) 317, 2281, 3239
BspHI TCATGA 1 cut(s) 3145
BspMAI CTGCAG 1 cut(s) 1791
BspMI ACCTGC 2 cut(s) 1922, 2310
BspPI GGATC 4 cut(s) 576, 1103, 2297, 2464
BspT104I TTCGAA 2 cut(s) 2254, 2362
BspT107I GGYRCC 3 cut(s) 1473, 2167, 2240
BsrDI GCAATG 4 cut(s) 1706, 1799, 2090, 2126
BsrI ACTGG 7 cut(s) 738, 879, 1927, 1958, 2750, 3218, 3366
BssECI CCNNGG 6 cut(s) 198, 315, 437, 916, 2162, 2490
BssT1I CCWWGG 1 cut(s) 916
Bst2UI CCWGG 2 cut(s) 1155, 3191
Bst4CI ACNGT 7 cut(s) 85, 998, 1049, 1088, 1345, 1526, 2540
Bst6I CTCTTC 1 cut(s) 2864
BstBAI YACGTR 2 cut(s) 1129, 1676
BstBI TTCGAA 2 cut(s) 2254, 2362
BstC8I GCNNGC 6 cut(s) 347, 600, 1806, 3027, 3086, 3235
BstDSI CCRYGG 2 cut(s) 315, 2490
BstFNI CGCG 3 cut(s) 317, 2281, 3239
BstHHI GCGC 1 cut(s) 3239
BstMAI GTCTC 3 cut(s) 1495, 1551, 2291
BstMCI CGRYCG 1 cut(s) 3206
BstNI CCWGG 2 cut(s) 1155, 3191
BstNSI RCATGY 5 cut(s) 2213, 2274, 2790, 2891, 2949
BstSCI CCNGG 4 cut(s) 197, 1153, 2826, 3189
BstSFI CTRYAG 3 cut(s) 888, 1787, 1891
BstSLI GKGCMC 1 cut(s) 1867
BstUI CGCG 3 cut(s) 317, 2281, 3239
BstX2I RGATCY 2 cut(s) 568, 1165
BstXI CCANNNNNNTGG 1 cut(s) 2214
BstYI RGATCY 2 cut(s) 568, 1165
Bsu36I CCTNAGG 1 cut(s) 42
BsuI GTATCC 1 cut(s) 819
BsuRI GGCC 5 cut(s) 442, 1394, 1507, 1627, 3011
BtgI CCRYGG 2 cut(s) 315, 2490
BtgZI GCGATG 4 cut(s) 135, 335, 2755, 2938
BtsI GCAGTG 1 cut(s) 3036
BtsIMutI CAGTG 5 cut(s) 731, 1578, 2757, 3036, 3272
BveI ACCTGC 2 cut(s) 1922, 2310
Cac8I GCNNGC 6 cut(s) 347, 600, 1806, 3027, 3086, 3235
CaiI CAGNNNCTG 2 cut(s) 65, 3260
CciI TCATGA 1 cut(s) 3145
CfoI GCGC 1 cut(s) 3239
Cfr13I GGNCC 5 cut(s) 706, 1506, 1585, 2340, 3010
Cfr42I CCGCGG 1 cut(s) 318
Csp6I GTAC 8 cut(s) 787, 1045, 1241, 2168, 2484, 3118, 3128, 3306
CviQI GTAC 8 cut(s) 787, 1045, 1241, 2168, 2484, 3118, 3128, 3306
DraI TTTAAA 4 cut(s) 618, 777, 1758, 1965
DraIII CACNNNGTG 1 cut(s) 866
Eam1104I CTCTTC 1 cut(s) 2864
EarI CTCTTC 1 cut(s) 2864
EciI GGCGGA 1 cut(s) 117
Ecl136II GAGCTC 2 cut(s) 1064, 1290
Eco130I CCWWGG 1 cut(s) 916
Eco147I AGGCCT 1 cut(s) 442
Eco24I GRGCYC 2 cut(s) 1066, 1292
Eco32I GATATC 5 cut(s) 630, 740, 1981, 2610, 3067
Eco47I GGWCC 3 cut(s) 706, 1585, 2340
Eco53kI GAGCTC 2 cut(s) 1064, 1290
Eco57I CTGAAG 5 cut(s) 87, 296, 767, 1212, 1422
Eco72I CACGTG 2 cut(s) 1129, 1676
Eco81I CCTNAGG 1 cut(s) 42
Eco88I CYCGRG 2 cut(s) 133, 2163
EcoICRI GAGCTC 2 cut(s) 1064, 1290
EcoRI GAATTC 1 cut(s) 184
EcoRII CCWGG 2 cut(s) 1153, 3189
EcoRV GATATC 5 cut(s) 630, 740, 1981, 2610, 3067
EcoT14I CCWWGG 1 cut(s) 916
EcoT22I ATGCAT 2 cut(s) 1387, 2272
EcoT38I GRGCYC 2 cut(s) 1066, 1292
ErhI CCWWGG 1 cut(s) 916
Esp3I CGTCTC 1 cut(s) 1495
FalI AAGNNNNNCTT 6 cut(s) 1155, 1187, 1671, 1703, 2337, 2369
FbaI TGATCA 1 cut(s) 1231
FriOI GRGCYC 2 cut(s) 1066, 1292
FspBI CTAG 4 cut(s) 1248, 1338, 1518, 1640
GlaI GCGC 1 cut(s) 3238
GsuI CTGGAG 3 cut(s) 516, 752, 3212
HaeIII GGCC 5 cut(s) 442, 1394, 1507, 1627, 3011
HapII CCGG 7 cut(s) 153, 198, 368, 2338, 2443, 2827, 3320
HhaI GCGC 1 cut(s) 3239
Hin6I GCGC 1 cut(s) 3237
HinP1I GCGC 1 cut(s) 3237
HincII GTYRAC 4 cut(s) 1463, 3102, 3180, 3289
HindII GTYRAC 4 cut(s) 1463, 3102, 3180, 3289
HindIII AAGCTT 2 cut(s) 160, 794
HpaI GTTAAC 1 cut(s) 3180
HpaII CCGG 7 cut(s) 153, 198, 368, 2338, 2443, 2827, 3320
HphI GGTGA 7 cut(s) 481, 878, 1241, 1427, 1759, 2747, 2816
Hpy166II GTNNAC 5 cut(s) 1463, 1693, 3102, 3180, 3289
Hpy8I GTNNAC 5 cut(s) 1463, 1693, 3102, 3180, 3289
Hpy99I CGWCG 4 cut(s) 131, 367, 1393, 2034
HpyCH4III ACNGT 7 cut(s) 85, 998, 1049, 1088, 1345, 1526, 2540
HpyCH4IV ACGT 3 cut(s) 222, 1128, 1675
HpySE526I ACGT 3 cut(s) 222, 1128, 1675
HspAI GCGC 1 cut(s) 3237
Kpn2I TCCGGA 1 cut(s) 3319
KpnI GGTACC 1 cut(s) 2171
Ksp22I TGATCA 1 cut(s) 1231
KspAI GTTAAC 1 cut(s) 3180
KspI CCGCGG 1 cut(s) 318
LmnI GCTCC 6 cut(s) 104, 412, 1297, 2158, 2380, 2800
MaeI CTAG 4 cut(s) 1248, 1338, 1518, 1640
MaeII ACGT 3 cut(s) 222, 1128, 1675
MaeIII GTNAC 5 cut(s) 506, 1122, 1619, 2189, 2428
MflI RGATCY 2 cut(s) 568, 1165
MhlI GDGCHC 4 cut(s) 1066, 1292, 1852, 1867
MlyI GAGTC 7 cut(s) 125, 1328, 1336, 1664, 3097, 3220, 3266
MmeI TCCRAC 6 cut(s) 104, 369, 675, 1695, 3072, 3356
Mph1103I ATGCAT 2 cut(s) 1387, 2272
MroI TCCGGA 1 cut(s) 3319
MroXI GAANNNNTTC 1 cut(s) 1683
MslI CAYNNNNRTG 5 cut(s) 1479, 1688, 2212, 2451, 2829
MspA1I CMGCKG 2 cut(s) 110, 317
MspI CCGG 7 cut(s) 153, 198, 368, 2338, 2443, 2827, 3320
MspR9I CCNGG 4 cut(s) 199, 1155, 2828, 3191
Mva1269I GAATGC 2 cut(s) 347, 2806
MvaI CCWGG 2 cut(s) 1155, 3191
MvnI CGCG 3 cut(s) 317, 2281, 3239
NciI CCSGG 2 cut(s) 199, 2828
NmeAIII GCCGAG 2 cut(s) 454, 462
NmuCI GTSAC 2 cut(s) 506, 1619
NsiI ATGCAT 2 cut(s) 1387, 2272
NspI RCATGY 5 cut(s) 2213, 2274, 2790, 2891, 2949
NspV TTCGAA 2 cut(s) 2254, 2362
OliI CACNNNNGTG 1 cut(s) 2212
PaeR7I CTCGAG 1 cut(s) 133
PagI TCATGA 1 cut(s) 3145
PaqCI CACCTGC 1 cut(s) 1922
PceI AGGCCT 1 cut(s) 442
PciI ACATGT 1 cut(s) 2887
PctI GAATGC 2 cut(s) 347, 2806
PdmI GAANNNNTTC 1 cut(s) 1683
PfeI GAWTC 7 cut(s) 872, 1181, 1750, 2251, 3142, 3316, 3325
PflFI GACNNNGTC 1 cut(s) 676
PflMI CCANNNNNTGG 2 cut(s) 539, 1744
PleI GAGTC 7 cut(s) 125, 1328, 1335, 1664, 3097, 3220, 3265
PmaCI CACGTG 2 cut(s) 1129, 1676
PmlI CACGTG 2 cut(s) 1129, 1676
PpsI GAGTC 7 cut(s) 125, 1328, 1335, 1664, 3097, 3220, 3265
Ppu21I YACGTR 2 cut(s) 1129, 1676
PscI ACATGT 1 cut(s) 2887
PshBI ATTAAT 1 cut(s) 297
Psp124BI GAGCTC 2 cut(s) 1066, 1292
Psp6I CCWGG 2 cut(s) 1153, 3189
PspCI CACGTG 2 cut(s) 1129, 1676
PspGI CCWGG 2 cut(s) 1153, 3189
PspPI GGNCC 5 cut(s) 706, 1506, 1585, 2340, 3010
PspXI VCTCGAGB 1 cut(s) 133
PsrI GAACNNNNNNTAC 2 cut(s) 2516, 2548
PstI CTGCAG 1 cut(s) 1791
PstNI CAGNNNCTG 2 cut(s) 65, 3260
PsuI RGATCY 2 cut(s) 568, 1165
PsyI GACNNNGTC 1 cut(s) 676
RsaI GTAC 8 cut(s) 788, 1046, 1242, 2169, 2485, 3119, 3129, 3307
RsaNI GTAC 8 cut(s) 787, 1045, 1241, 2168, 2484, 3118, 3128, 3306
RseI CAYNNNNRTG 5 cut(s) 1479, 1688, 2212, 2451, 2829
SacI GAGCTC 2 cut(s) 1066, 1292
SacII CCGCGG 1 cut(s) 318
Sau96I GGNCC 5 cut(s) 706, 1506, 1585, 2340, 3010
SchI GAGTC 7 cut(s) 125, 1328, 1336, 1664, 3097, 3220, 3266
ScrFI CCNGG 4 cut(s) 199, 1155, 2828, 3191
SduI GDGCHC 4 cut(s) 1066, 1292, 1852, 1867
SfcI CTRYAG 3 cut(s) 888, 1787, 1891
Sfr274I CTCGAG 1 cut(s) 133
Sfr303I CCGCGG 1 cut(s) 318
SfuI TTCGAA 2 cut(s) 2254, 2362
SgrBI CCGCGG 1 cut(s) 318
SinI GGWCC 3 cut(s) 706, 1585, 2340
SlaI CTCGAG 1 cut(s) 133
SmiI ATTTAAAT 1 cut(s) 618
SmiMI CAYNNNNRTG 5 cut(s) 1479, 1688, 2212, 2451, 2829
SmlI CTYRAG 5 cut(s) 133, 416, 479, 3228, 3245
SmoI CTYRAG 5 cut(s) 133, 416, 479, 3228, 3245
SseBI AGGCCT 1 cut(s) 442
SsiI CCGC 9 cut(s) 102, 108, 262, 315, 317, 434, 1120, 2279, 3239
SspI AATATT 3 cut(s) 1270, 2221, 2312
SspMI CTAG 4 cut(s) 1248, 1338, 1518, 1640
SstI GAGCTC 2 cut(s) 1066, 1292
StuI AGGCCT 1 cut(s) 442
StyD4I CCNGG 4 cut(s) 197, 1153, 2826, 3189
StyI CCWWGG 1 cut(s) 916
SwaI ATTTAAAT 1 cut(s) 618
TaaI ACNGT 7 cut(s) 85, 998, 1049, 1088, 1345, 1526, 2540
TaiI ACGT 3 cut(s) 225, 1131, 1678
TaqI TCGA 8 cut(s) 134, 144, 173, 245, 671, 2254, 2362, 2929
TaqII GACCGA 1 cut(s) 3192
TatI WGTACW 2 cut(s) 786, 1240
TauI GCSGC 4 cut(s) 110, 265, 320, 436
TfiI GAWTC 7 cut(s) 872, 1181, 1750, 2251, 3142, 3316, 3325
TscAI CASTG 5 cut(s) 738, 1585, 2757, 3036, 3279
TseFI GTSAC 2 cut(s) 506, 1619
Tsp45I GTSAC 2 cut(s) 506, 1619
TspGWI ACGGA 5 cut(s) 2416, 2479, 2525, 3105, 3323
TspRI CASTG 5 cut(s) 738, 1585, 2757, 3036, 3279
Tth111I GACNNNGTC 1 cut(s) 676
Van91I CCANNNNNTGG 2 cut(s) 539, 1744
VpaK11BI GGWCC 3 cut(s) 706, 1585, 2340
VspI ATTAAT 1 cut(s) 297
XapI RAATTY 4 cut(s) 184, 1658, 2503, 3111
XceI RCATGY 5 cut(s) 2213, 2274, 2790, 2891, 2949
XcmI CCANNNNNNNNNTGG 1 cut(s) 1595
XhoI CTCGAG 1 cut(s) 133
XmnI GAANNNNTTC 1 cut(s) 1683
XspI CTAG 4 cut(s) 1248, 1338, 1518, 1640
Zsp2I ATGCAT 2 cut(s) 1387, 2272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.