Rw4G001410

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0007173
Physical Location & Seq
Forward (+)
2533076 .. 2541627
8552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G001410.1

Sequence Viewer

Length: 3447 bp
ATGCAAGTCCCCAATTCTGAAGAAACCGCCCCCGTAAACCCCCAAACCCCTAAAGACCCCGAAACCTCCGATTCCAATTCGGCAGTGGACGACGATGACAGTTGCGCTGCAGACGACGAGTCGTCGGTTCTCGATGTCTCCGGCAAAAGCTTGGATTTTTCGATTGGGGAGAATTCCGGCGACGGCGTCGGGGCTTTGTATTTGTACAAAAATGTGTATAATTTATTGCCCAAGTCCGTCGGGAGGCTGAAGCGGCTGAGGACGTTGAAGTTTTTCGGGAATGAGATTAATTTGTTCTCTTCGTCGGAGTTTGGGAACTTGGTGGGATTGGAATGCTTGCAGCTCCGGCTATCGTCGCCGGCGTTTGATGGTCTGCCATTGCATAAATTTAAAGGCTTGAAGGAGCTGGAGCTTTCGAAAGTGCCTTCTCGGTCCTCGGCTATACCGATACTGAGTGAGATTGCTAGGCTCAACTGTCTCACCAAGCTCTCTGTTTGTTACTTTTCTATAAGATACCTTCCTCCTGAAATTGGCTGCTTAAGCAAGTTGGAATACCTAGATCTTTCATTCAATAAGATGAAGAGTTTGCCAGCTGAAATTAGTAATTTAAATGCCTTGATATCGTTAAAAGTTGCCAATAATAAATTATCAGAACTACCTTTGGCTTTGTCCTCGTTGAAAATGTTGGAGAATTTGGACCTGTCGCACAACAGGTTGACATCATTGGGGTCCCTTGATCTTAGCTTGATGCGAAACCTCCAGAATTTAAATCTTCAGCACAACATGCTTCCCATCTACTGTCAAATACCTTCATGGATATGTTGCAATTTCGAAGGTAATGGAAAAGATATTTCCAACGACGATTTTAGCAGTACCTCAGTTGAAATGGATGTATATGAAACCACCATTCAGAAGAATGATGAAAATCGTTTTCACACAGGCTTTCATCATAGCTCATCGAGTATAGTTGGGCCTTCATCTAACAGTAGGTGTTTCACAACTCGGAGATCAGCTGGCCGGTGGAAGCGACAGTTCTTGCAGAGGAGAGCTCGTCAAGAACGCTTAAATAACAGCAGGAAGTCGAAAGGTCTGGATCTTCCTAAGCTGCACATGAAGGAGGATGAAGACTGCAAACCGGGCAATATAGATGCTACTTTTGAATCATATCGTGAAAGTGCACTGGACGTTATAAATTTGGATGATGATAATGATAAAAGTTTACTTTCTGGAGAAGAAGAAGGTGTAAACGTCTCCCATGCTGCACATCATGACATGTGTTCGAAGGAGGAGCTTTCTGTAAGAAGTTGCTCATCTCTCAGGGTTAACTCTACTCTAGTAGATAATGGTGATAAAAAGGATTGTTACGAGAGTGATGCATCGTCAACTTGCAATCAAGAGGTTACTGGTGAGCATGATGACGTGCCATATTCAGGAAAATCCAAATGCAGTTCAAAGTCCAAGAGGCCACGAGATGGGGATCTTGATAATAGGAATCTTCAGGATCCCAAACGTTGGAAGTGTGGAGATTGTAGTTCAAGCCTATCTTGCAGAAACATGTCTTGCAAATATAGTAATATGTCATTTTGCAGTGCTGAGGACCATCTTCCAGATGGCTTTTATGATGCAGGACGTGACAGGCCCTTCATGCCACTAGAGAGTTATGAGCAAATTTTCCATCTCGACTCACGTGAAGTTATTCTTGTGGACAGGCTAAGGGATAAAGAGTTGGATGGAATTTTGTGCTCTGCCCGAGATATGGTGTCTCAATTGAAGCAGTTAAATGGTTTAAATACAGATAGAGATCGGGATGATGAGCTACAGATTGCATTATATCTTGCTCTATTTGTATCAGATCATTTTGGAGGGACTGATAGGGCGGCTCTTGTTGAAAGGAGACGAAGAGCTGGTGGTGGTTCAATATCCAGGAAGCCTTTTGTATGCACCTGCTCAATTAGGAACTCTGAATCTATTAGTTTGAACCCCAAACAGTCTCCGGAGACTGTTGAAGATATTGCCTTTTCTGATATCTGTGAGAAATCTCTCCGTTCTATCAAAGCCAGACGAAAATCCGTTGTAGTACCTATAGGAACATTGCAGTTTGGTGTTTGTAGGCATAGAGCATTACTACTGAAGTACCTATGTGATCGGATGGAACCTCCAGTCCCTTGTGAGCTTGTCAGAGGATACCTGGATTTCATGCCACATGCTTGGAATATTATATCAGTCAAGAAAAATGACTCAGCCAAGAGAGGTGACTCAATTAAGGGAGGTGACTTGGTTGAGAGATATGACTCACGGATTCGCATGATTGTTGATGCATGTCGTCCGCATGATGTAAGAGAGGAGACAGATCCTGAATATTATTGCAGGTATATTCCTCTCAGTCGGGCCAAGGTTTCTTTCTCTGGAAGTAGCCCTGCTGATGTTTGTTTGTTTCCTTCATTGTCCTCTTCGGATGAAACTCAAACTGAATCCGGTAGTTCTCTCATTCGGTGTAAATATGGGTCGGCTGACGCAGCAGCAAAGATGCGTACTCTGGAGGTATATGGGACCTCAGTGGATGATATTAGGAACTTTGAATACAGCTGCTTGGGTGAAGTTAGAATTTTAGGTGCTTTGCAACACCCCTGCATAGTAGAGATGTATGGACACAGTATATCATCCAAGTGGGCTCCTTCTATGGATGATAATTCTGGGCACCGTATATTGCAATCTGTAATTTTCATGGAGTATATAAACGGGGGTTCCTTAAAGGGTTACATAGAAAAGCTATCTAAAGCAGGTGAGAAGCATGTCCCTGTGGAGTTAGCTTTAAGCATTGCTAAGAATGTTGCATGTGCACTGGTGGAGTTGCACTCAAAACACATTATTCATCGTGACATAAAAAGTGCAAACATTTTGATTGATATCGATAGGAAGACAGCTGATGGAACACCTGTTGTGAAGCTCTGTGATTTTGATAGGGCAGTGCCTCTTAGCTCGTACTTGCATACATGCTGTATTGCCCATGTTGGGATACCTCCTCCTGATGTTTGTGTTGGAACACCTCGCTGGATGGCTCCAGAGGTTCTGCGGGCAATGCATAAACAAAATATTTATGGATTGGAAGTTGATATCTGGTCGTTCGGATGCCTTCTTTTGGAGATGTTGACTTTGCAGATTCCTTACTTGGGATTATCTGAATTAGAGATTCATGACTGCCTATCGATGGGAAAACGACCCAAGTTAACAGATAACTTGGAGGCGCTTCGGCCATTGGAGAAGCCTGCAATGGCTCAATCTGCTGAAGAGCTTGAGCAAACAGAAGCTGACTTAGATGTACTAAGATTTCTGGTTGACTTGTTCTGCCAATGTACTGAGGAGAATCCGCAAAGTCGTCCAACGGCTGACAGCCTCTACAAATTGTTGCTCGAACGCAGCACCCAGTTTACAAGTTCAAGAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

1148

Amino Acids

128.29

Weight (kDa)

5.74

Isoelectric Point (pI)

52.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 120 - 259 2.4e-12 Leucine-rich repeat region
EDR1_CTR1_ARMC3_pept PF14381 675 - 742 4.4e-11 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 861 - 1133 1.1e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 862 - 1133 3.5e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1190
AarI CACCTGC 2 cut(s) 1952, 2771
Acc36I ACCTGC 3 cut(s) 1952, 2358, 2771
AccB1I GGYRCC 1 cut(s) 2697
AccB7I CCANNNNNTGG 2 cut(s) 1472, 1512
AccIII TCCGGA 1 cut(s) 1993
AciI CCGC 6 cut(s) 27, 253, 1877, 2327, 3073, 3368
AclI AACGTT 1 cut(s) 1510
AclWI GGATC 5 cut(s) 1101, 1485, 1496, 1509, 2345
AcoI YGGCCR 2 cut(s) 1015, 3251
AcsI RAATTY 8 cut(s) 172, 386, 691, 763, 1192, 1668, 1734, 2604
AcuI CTGAAG 6 cut(s) 39, 269, 758, 1481, 2150, 3306
AcvI CACGTG 1 cut(s) 1688
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 8 cut(s) 206, 874, 2079, 2135, 2533, 2984, 3321, 3355
AflII CTTAAG 1 cut(s) 538
AflIII ACRYGT 2 cut(s) 1272, 1554
AhdI GACNNNNNGTC 2 cut(s) 118, 121
AjiI CACGTC 2 cut(s) 1420, 1631
AjnI CCWGG 2 cut(s) 1922, 2187
AjuI GAANNNNNNNTTGG 2 cut(s) 2384, 2416
AloI GAACNNNNNNTCC 2 cut(s) 2145, 2177
Alw21I GWGCWC 4 cut(s) 1051, 1180, 1745, 2842
Alw26I GTCTC 8 cut(s) 142, 482, 1255, 1767, 1888, 1991, 1995, 2339
Alw44I GTGCAC 2 cut(s) 1176, 2838
AlwI GGATC 5 cut(s) 1101, 1485, 1496, 1509, 2345
AlwNI CAGNNNCTG 2 cut(s) 2354, 3308
Ama87I CYCGRG 1 cut(s) 1749
Aor13HI TCCGGA 1 cut(s) 1993
AoxI GGCC 6 cut(s) 971, 1015, 1463, 1637, 2388, 3251
ApaLI GTGCAC 2 cut(s) 1176, 2838
ApeKI GCWGC 9 cut(s) 107, 340, 534, 1105, 1259, 2516, 2519, 2586, 3417
ApoI RAATTY 8 cut(s) 172, 386, 691, 763, 1192, 1668, 1734, 2604
AseI ATTAAT 1 cut(s) 288
Asp700I GAANNNNTTC 2 cut(s) 272, 1695
AspLEI GCGC 2 cut(s) 107, 3247
AspS9I GGNCC 8 cut(s) 432, 697, 729, 971, 1597, 1638, 2388, 2550
AsuC2I CCSGG 1 cut(s) 1137
AsuHPI GGTGA 7 cut(s) 472, 1358, 1418, 2264, 2282, 2606, 2795
AsuII TTCGAA 3 cut(s) 416, 831, 1280
AvaI CYCGRG 1 cut(s) 1749
AvaII GGWCC 5 cut(s) 432, 697, 729, 1597, 2550
BaeGI GKGCMC 3 cut(s) 1180, 2700, 2842
BaeI ACNNNNGTAYC 2 cut(s) 2117, 2150
BamHI GGATCC 1 cut(s) 1501
BanI GGYRCC 1 cut(s) 2697
BanII GRGCYC 2 cut(s) 1051, 2674
BauI CACGAG 1 cut(s) 1467
BbrPI CACGTG 1 cut(s) 1688
BbsI GAAGAC 2 cut(s) 1131, 2924
Bbv12I GWGCWC 4 cut(s) 1051, 1180, 1745, 2842
BbvCI CCTCAGC 2 cut(s) 257, 1593
BbvI GCAGC 9 cut(s) 94, 352, 521, 1092, 1246, 2528, 2531, 2573, 3429
BceAI ACGGC 2 cut(s) 199, 3399
BcgI CGANNNNNNTGC 2 cut(s) 1355, 1389
BciT130I CCWGG 2 cut(s) 1924, 2189
BciVI GTATCC 2 cut(s) 2177, 3009
BcnI CCSGG 1 cut(s) 1137
BcoDI GTCTC 8 cut(s) 142, 482, 1255, 1767, 1888, 1991, 1995, 2339
BfaI CTAG 4 cut(s) 465, 557, 1334, 1652
BfmI CTRYAG 3 cut(s) 108, 1817, 2082
BfoI RGCGCY 1 cut(s) 3248
BfrI CTTAAG 1 cut(s) 538
BfuAI ACCTGC 3 cut(s) 1952, 2358, 2771
BfuI GTATCC 2 cut(s) 2177, 3009
BglII AGATCT 1 cut(s) 559
Bme1390I CCNGG 3 cut(s) 1137, 1924, 2189
Bme18I GGWCC 5 cut(s) 432, 697, 729, 1597, 2550
BmeRI GACNNNNNGTC 2 cut(s) 118, 121
BmeT110I CYCGRG 1 cut(s) 1749
BmgBI CACGTC 2 cut(s) 1420, 1631
BmgT120I GGNCC 8 cut(s) 432, 697, 729, 971, 1597, 1638, 2388, 2550
BmiI GGNNCC 9 cut(s) 730, 731, 1503, 2154, 2551, 2673, 2699, 2746, 3060
BmrFI CCNGG 3 cut(s) 1137, 1924, 2189
BmrI ACTGGG 1 cut(s) 3418
BmsI GCATC 8 cut(s) 738, 1138, 1363, 1385, 1612, 2305, 2517, 3119
BmuI ACTGGG 1 cut(s) 3418
BpiI GAAGAC 2 cut(s) 1131, 2924
BplI GAGNNNNNCTC 4 cut(s) 1033, 1065, 2840, 2872
BpmI CTGGAG 6 cut(s) 428, 743, 1248, 2142, 2558, 3045
Bpu10I CCTNAGC 4 cut(s) 257, 1101, 1593, 1712
Bpu14I TTCGAA 3 cut(s) 416, 831, 1280
BpuEI CTTGAG 1 cut(s) 3314
BpuMI CCSGG 1 cut(s) 1137
Bsa29I ATCGAT 2 cut(s) 2910, 3206
BsaAI YACGTR 1 cut(s) 1688
BsaBI GATNNNNATC 4 cut(s) 924, 1476, 1800, 2906
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 2 cut(s) 435, 2391
BsaWI WCCGGW 2 cut(s) 1993, 2474
BsaXI ACNNNNNCTCC 2 cut(s) 1036, 1066
Bse118I RCCGGY 2 cut(s) 358, 1017
Bse1I ACTGG 5 cut(s) 1185, 1408, 2159, 2847, 3424
Bse3DI GCAATG 5 cut(s) 377, 2090, 2817, 3084, 3276
Bse8I GATNNNNATC 4 cut(s) 924, 1476, 1800, 2906
BseAI TCCGGA 1 cut(s) 1993
BseBI CCWGG 2 cut(s) 1924, 2189
BseCI ATCGAT 2 cut(s) 2910, 3206
BseDI CCNNGG 2 cut(s) 435, 2391
BseJI GATNNNNATC 4 cut(s) 924, 1476, 1800, 2906
BseMI GCAATG 5 cut(s) 377, 2090, 2817, 3084, 3276
BseMII CTCAG 9 cut(s) 248, 443, 891, 1330, 1584, 2253, 2395, 2568, 3348
BseNI ACTGG 5 cut(s) 1185, 1408, 2159, 2847, 3424
BseRI GAGGAG 5 cut(s) 1057, 1301, 2357, 3012, 3374
BseSI GKGCMC 3 cut(s) 1180, 2700, 2842
BseXI GCAGC 9 cut(s) 94, 352, 521, 1092, 1246, 2528, 2531, 2573, 3429
BsgI GTGCAG 2 cut(s) 1091, 1245
BshFI GGCC 6 cut(s) 973, 1017, 1465, 1639, 2390, 3253
BshNI GGYRCC 1 cut(s) 2697
BshVI ATCGAT 2 cut(s) 2910, 3206
BsiHKAI GWGCWC 4 cut(s) 1051, 1180, 1745, 2842
BsiHKCI CYCGRG 1 cut(s) 1749
BsiSI CCGG 8 cut(s) 141, 177, 346, 359, 1018, 1136, 1994, 2475
BslFI GGGAC 5 cut(s) 715, 1879, 2147, 2563, 2780
BsmAI GTCTC 8 cut(s) 142, 482, 1255, 1767, 1888, 1991, 1995, 2339
BsmBI CGTCTC 2 cut(s) 1255, 1888
BsmFI GGGAC 5 cut(s) 715, 1879, 2147, 2563, 2780
BsmI GAATGC 1 cut(s) 338
BsnI GGCC 6 cut(s) 973, 1017, 1465, 1639, 2390, 3253
BsoBI CYCGRG 1 cut(s) 1749
Bsp119I TTCGAA 3 cut(s) 416, 831, 1280
Bsp1286I GDGCHC 6 cut(s) 1051, 1180, 1745, 2674, 2700, 2842
Bsp13I TCCGGA 1 cut(s) 1993
Bsp1407I TGTACA 1 cut(s) 204
BspACI CCGC 6 cut(s) 27, 253, 1877, 2327, 3073, 3368
BspANI GGCC 6 cut(s) 973, 1017, 1465, 1639, 2390, 3253
BspCNI CTCAG 9 cut(s) 249, 444, 890, 1329, 1585, 2252, 2394, 2567, 3349
BspDI ATCGAT 2 cut(s) 2910, 3206
BspEI TCCGGA 1 cut(s) 1993
BspHI TCATGA 2 cut(s) 1267, 3193
BspLI GGNNCC 9 cut(s) 730, 731, 1503, 2154, 2551, 2673, 2699, 2746, 3060
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 3 cut(s) 1952, 2358, 2771
BspPI GGATC 5 cut(s) 1101, 1485, 1496, 1509, 2345
BspQI GCTCTTC 2 cut(s) 1894, 3282
BspT104I TTCGAA 3 cut(s) 416, 831, 1280
BspT107I GGYRCC 1 cut(s) 2697
BspTI CTTAAG 1 cut(s) 538
BsrDI GCAATG 5 cut(s) 377, 2090, 2817, 3084, 3276
BsrFI RCCGGY 2 cut(s) 358, 1017
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 5 cut(s) 1185, 1408, 2159, 2847, 3424
BssAI RCCGGY 2 cut(s) 358, 1017
BssECI CCNNGG 2 cut(s) 435, 2391
BssNI GRCGYC 1 cut(s) 186
BssSI CACGAG 1 cut(s) 1467
BssT1I CCWWGG 1 cut(s) 2391
Bst2BI CACGAG 1 cut(s) 1467
Bst2UI CCWGG 2 cut(s) 1924, 2189
Bst4CI ACNGT 9 cut(s) 101, 476, 800, 986, 1032, 1989, 2002, 2654, 2702
Bst6I CTCTTC 5 cut(s) 304, 575, 1894, 2455, 3282
BstACI GRCGYC 1 cut(s) 186
BstAFI CTTAAG 1 cut(s) 538
BstAPI GCANNNNNTGC 1 cut(s) 784
BstAUI TGTACA 1 cut(s) 204
BstBAI YACGTR 1 cut(s) 1688
BstBI TTCGAA 3 cut(s) 416, 831, 1280
BstC8I GCNNGC 6 cut(s) 338, 360, 591, 1015, 3075, 3267
BstH2I RGCGCY 1 cut(s) 3248
BstHHI GCGC 2 cut(s) 107, 3247
BstMAI GTCTC 8 cut(s) 142, 482, 1255, 1767, 1888, 1991, 1995, 2339
BstNI CCWGG 2 cut(s) 1924, 2189
BstNSI RCATGY 8 cut(s) 787, 1276, 1558, 2207, 2322, 2795, 2838, 2997
BstSCI CCNGG 3 cut(s) 1135, 1922, 2187
BstSFI CTRYAG 3 cut(s) 108, 1817, 2082
BstSLI GKGCMC 3 cut(s) 1180, 2700, 2842
BstV1I GCAGC 9 cut(s) 94, 352, 521, 1092, 1246, 2528, 2531, 2573, 3429
BstV2I GAAGAC 2 cut(s) 1131, 2924
BstX2I RGATCY 5 cut(s) 559, 1093, 1477, 1501, 2350
BstXI CCANNNNNNTGG 1 cut(s) 2208
BstYI RGATCY 5 cut(s) 559, 1093, 1477, 1501, 2350
Bsu15I ATCGAT 2 cut(s) 2910, 3206
BsuI GTATCC 2 cut(s) 2177, 3009
BsuRI GGCC 6 cut(s) 973, 1017, 1465, 1639, 2390, 3253
BsuTUI ATCGAT 2 cut(s) 2910, 3206
BtrI CACGTC 2 cut(s) 1420, 1631
BtsI GCAGTG 3 cut(s) 90, 1594, 2973
BtsIMutI CAGTG 6 cut(s) 90, 1178, 1594, 2562, 2840, 2973
BveI ACCTGC 3 cut(s) 1952, 2358, 2771
Cac8I GCNNGC 6 cut(s) 338, 360, 591, 1015, 3075, 3267
CaiI CAGNNNCTG 2 cut(s) 2354, 3308
CciI TCATGA 2 cut(s) 1267, 3193
CfoI GCGC 2 cut(s) 107, 3247
Cfr10I RCCGGY 2 cut(s) 358, 1017
Cfr13I GGNCC 8 cut(s) 432, 697, 729, 971, 1597, 1638, 2388, 2550
ClaI ATCGAT 2 cut(s) 2910, 3206
CseI GACGC 2 cut(s) 175, 2522
Csp6I GTAC 8 cut(s) 205, 873, 2078, 2134, 2532, 2983, 3320, 3354
CviQI GTAC 8 cut(s) 205, 873, 2078, 2134, 2532, 2983, 3320, 3354
DraI TTTAAA 4 cut(s) 391, 609, 768, 1788
DriI GACNNNNNGTC 2 cut(s) 118, 121
EaeI YGGCCR 2 cut(s) 1015, 3251
Eam1104I CTCTTC 5 cut(s) 304, 575, 1894, 2455, 3282
Eam1105I GACNNNNNGTC 2 cut(s) 118, 121
EarI CTCTTC 5 cut(s) 304, 575, 1894, 2455, 3282
Ecl136II GAGCTC 1 cut(s) 1049
Eco130I CCWWGG 1 cut(s) 2391
Eco24I GRGCYC 2 cut(s) 1051, 2674
Eco32I GATATC 4 cut(s) 621, 2026, 2908, 3115
Eco47I GGWCC 5 cut(s) 432, 697, 729, 1597, 2550
Eco53kI GAGCTC 1 cut(s) 1049
Eco57I CTGAAG 6 cut(s) 39, 269, 758, 1481, 2150, 3306
Eco72I CACGTG 1 cut(s) 1688
Eco88I CYCGRG 1 cut(s) 1749
EcoICRI GAGCTC 1 cut(s) 1049
EcoO109I RGGNCCY 3 cut(s) 729, 1638, 2550
EcoRI GAATTC 1 cut(s) 172
EcoRII CCWGG 2 cut(s) 1922, 2187
EcoRV GATATC 4 cut(s) 621, 2026, 2908, 3115
EcoT14I CCWWGG 1 cut(s) 2391
EcoT22I ATGCAT 3 cut(s) 1378, 2320, 3084
EcoT38I GRGCYC 2 cut(s) 1051, 2674
ErhI CCWWGG 1 cut(s) 2391
Esp3I CGTCTC 2 cut(s) 1255, 1888
FalI AAGNNNNNCTT 4 cut(s) 1528, 1560, 1683, 1715
FaqI GGGAC 5 cut(s) 715, 1879, 2147, 2563, 2780
FauI CCCGC 1 cut(s) 3066
FriOI GRGCYC 2 cut(s) 1051, 2674
FspBI CTAG 4 cut(s) 465, 557, 1334, 1652
GlaI GCGC 2 cut(s) 106, 3246
GsuI CTGGAG 6 cut(s) 428, 743, 1248, 2142, 2558, 3045
HaeII RGCGCY 1 cut(s) 3248
HaeIII GGCC 6 cut(s) 973, 1017, 1465, 1639, 2390, 3253
HapII CCGG 8 cut(s) 141, 177, 346, 359, 1018, 1136, 1994, 2475
HgaI GACGC 2 cut(s) 175, 2522
HhaI GCGC 2 cut(s) 107, 3247
Hin1I GRCGYC 1 cut(s) 186
Hin6I GCGC 2 cut(s) 105, 3245
HinP1I GCGC 2 cut(s) 105, 3245
HincII GTYRAC 6 cut(s) 717, 1324, 1383, 3150, 3228, 3337
HindII GTYRAC 6 cut(s) 717, 1324, 1383, 3150, 3228, 3337
HindIII AAGCTT 1 cut(s) 148
HpaI GTTAAC 2 cut(s) 1324, 3228
HpaII CCGG 8 cut(s) 141, 177, 346, 359, 1018, 1136, 1994, 2475
HphI GGTGA 7 cut(s) 472, 1358, 1418, 2264, 2282, 2606, 2795
Hpy99I CGWCG 9 cut(s) 95, 119, 127, 185, 191, 242, 307, 358, 863
HpyCH4III ACNGT 9 cut(s) 101, 476, 800, 986, 1032, 1989, 2002, 2654, 2702
HpyCH4IV ACGT 7 cut(s) 263, 1185, 1248, 1419, 1510, 1630, 1687
HpySE526I ACGT 7 cut(s) 263, 1185, 1248, 1419, 1510, 1630, 1687
Hsp92I GRCGYC 1 cut(s) 186
HspAI GCGC 2 cut(s) 105, 3245
KflI GGGWCCC 1 cut(s) 729
Kpn2I TCCGGA 1 cut(s) 1993
KroI GCCGGC 1 cut(s) 358
KroNI GCCGGC 1 cut(s) 360
KspAI GTTAAC 2 cut(s) 1324, 3228
LguI GCTCTTC 2 cut(s) 1894, 3282
LmnI GCTCC 6 cut(s) 348, 403, 409, 1288, 2677, 3064
Lsp1109I GCAGC 9 cut(s) 94, 352, 521, 1092, 1246, 2528, 2531, 2573, 3429
LweI GCATC 8 cut(s) 738, 1138, 1363, 1385, 1612, 2305, 2517, 3119
MaeI CTAG 4 cut(s) 465, 557, 1334, 1652
MaeII ACGT 7 cut(s) 263, 1185, 1248, 1419, 1510, 1630, 1687
MaeIII GTNAC 8 cut(s) 497, 1361, 1399, 1631, 2252, 2270, 2756, 2876
MfeI CAATTG 1 cut(s) 1766
MflI RGATCY 5 cut(s) 559, 1093, 1477, 1501, 2350
MhlI GDGCHC 6 cut(s) 1051, 1180, 1745, 2674, 2700, 2842
MlyI GAGTC 5 cut(s) 128, 1676, 2231, 2249, 2285
MmeI TCCRAC 8 cut(s) 285, 528, 666, 879, 1493, 1707, 3019, 3404
Mph1103I ATGCAT 3 cut(s) 1378, 2320, 3084
MreI CGCCGGCG 1 cut(s) 358
MroI TCCGGA 1 cut(s) 1993
MroNI GCCGGC 1 cut(s) 358
MroXI GAANNNNTTC 2 cut(s) 272, 1695
MslI CAYNNNNRTG 2 cut(s) 817, 2665
MspA1I CMGCKG 4 cut(s) 593, 1013, 2586, 2924
MspCI CTTAAG 1 cut(s) 538
MspI CCGG 8 cut(s) 141, 177, 346, 359, 1018, 1136, 1994, 2475
MspR9I CCNGG 3 cut(s) 1137, 1924, 2189
MunI CAATTG 1 cut(s) 1766
Mva1269I GAATGC 1 cut(s) 338
MvaI CCWGG 2 cut(s) 1924, 2189
NaeI GCCGGC 1 cut(s) 360
NciI CCSGG 1 cut(s) 1137
NgoMIV GCCGGC 1 cut(s) 358
NlaIV GGNNCC 9 cut(s) 730, 731, 1503, 2154, 2551, 2673, 2699, 2746, 3060
NmeAIII GCCGAG 1 cut(s) 416
NmuCI GTSAC 4 cut(s) 1631, 2252, 2270, 2876
NsiI ATGCAT 3 cut(s) 1378, 2320, 3084
NspI RCATGY 8 cut(s) 787, 1276, 1558, 2207, 2322, 2795, 2838, 2997
NspV TTCGAA 3 cut(s) 416, 831, 1280
PagI TCATGA 2 cut(s) 1267, 3193
PaqCI CACCTGC 2 cut(s) 1952, 2771
PciI ACATGT 2 cut(s) 1272, 1554
PciSI GCTCTTC 2 cut(s) 1894, 3282
PcsI WCGNNNNNNNCGW 2 cut(s) 359, 443
PctI GAATGC 1 cut(s) 338
PdiI GCCGGC 1 cut(s) 360
PdmI GAANNNNTTC 2 cut(s) 272, 1695
PfeI GAWTC 9 cut(s) 71, 1160, 1492, 1964, 2299, 2471, 3160, 3190, 3364
PflFI GACNNNGTC 1 cut(s) 185
PflMI CCANNNNNTGG 2 cut(s) 1472, 1512
PfoI TCCNGGA 1 cut(s) 1922
PleI GAGTC 5 cut(s) 127, 1676, 2231, 2249, 2285
PmaCI CACGTG 1 cut(s) 1688
PmlI CACGTG 1 cut(s) 1688
PpsI GAGTC 5 cut(s) 127, 1676, 2231, 2249, 2285
Ppu21I YACGTR 1 cut(s) 1688
PpuMI RGGWCCY 2 cut(s) 729, 2550
PscI ACATGT 2 cut(s) 1272, 1554
PshBI ATTAAT 1 cut(s) 288
PsiI TTATAA 1 cut(s) 1190
Psp124BI GAGCTC 1 cut(s) 1051
Psp1406I AACGTT 1 cut(s) 1510
Psp5II RGGWCCY 2 cut(s) 729, 2550
Psp6I CCWGG 2 cut(s) 1922, 2187
PspCI CACGTG 1 cut(s) 1688
PspGI CCWGG 2 cut(s) 1922, 2187
PspN4I GGNNCC 9 cut(s) 730, 731, 1503, 2154, 2551, 2673, 2699, 2746, 3060
PspPI GGNCC 8 cut(s) 432, 697, 729, 971, 1597, 1638, 2388, 2550
PspPPI RGGWCCY 2 cut(s) 729, 2550
PsrI GAACNNNNNNTAC 2 cut(s) 2564, 2596
PstI CTGCAG 1 cut(s) 112
PstNI CAGNNNCTG 2 cut(s) 2354, 3308
PsuI RGATCY 5 cut(s) 559, 1093, 1477, 1501, 2350
PsyI GACNNNGTC 1 cut(s) 185
PvuII CAGCTG 4 cut(s) 593, 1013, 2586, 2924
RsaI GTAC 8 cut(s) 206, 874, 2079, 2135, 2533, 2984, 3321, 3355
RsaNI GTAC 8 cut(s) 205, 873, 2078, 2134, 2532, 2983, 3320, 3354
RseI CAYNNNNRTG 2 cut(s) 817, 2665
SacI GAGCTC 1 cut(s) 1051
SapI GCTCTTC 2 cut(s) 1894, 3282
Sau96I GGNCC 8 cut(s) 432, 697, 729, 971, 1597, 1638, 2388, 2550
SchI GAGTC 5 cut(s) 128, 1676, 2231, 2249, 2285
ScrFI CCNGG 3 cut(s) 1137, 1924, 2189
SduI GDGCHC 6 cut(s) 1051, 1180, 1745, 2674, 2700, 2842
SfaNI GCATC 8 cut(s) 738, 1138, 1363, 1385, 1612, 2305, 2517, 3119
SfcI CTRYAG 3 cut(s) 108, 1817, 2082
SfuI TTCGAA 3 cut(s) 416, 831, 1280
SgrAI CRCCGGYG 1 cut(s) 358
SinI GGWCC 5 cut(s) 432, 697, 729, 1597, 2550
SmiI ATTTAAAT 2 cut(s) 609, 768
SmiMI CAYNNNNRTG 2 cut(s) 817, 2665
SmlI CTYRAG 2 cut(s) 538, 3293
SmoI CTYRAG 2 cut(s) 538, 3293
SsiI CCGC 6 cut(s) 27, 253, 1877, 2327, 3073, 3368
SspI AATATT 3 cut(s) 2215, 2360, 3094
SspMI CTAG 4 cut(s) 465, 557, 1334, 1652
SstI GAGCTC 1 cut(s) 1051
StyD4I CCNGG 3 cut(s) 1135, 1922, 2187
StyI CCWWGG 1 cut(s) 2391
SwaI ATTTAAAT 2 cut(s) 609, 768
TaaI ACNGT 9 cut(s) 101, 476, 800, 986, 1032, 1989, 2002, 2654, 2702
TaiI ACGT 7 cut(s) 266, 1188, 1251, 1422, 1513, 1633, 1690
TaqII GACCGA 1 cut(s) 420
TatI WGTACW 3 cut(s) 204, 3319, 3353
TauI GCSGC 2 cut(s) 256, 1880
TfiI GAWTC 9 cut(s) 71, 1160, 1492, 1964, 2299, 2471, 3160, 3190, 3364
TscAI CASTG 6 cut(s) 90, 1185, 1594, 2562, 2847, 2973
TseFI GTSAC 4 cut(s) 1631, 2252, 2270, 2876
TseI GCWGC 9 cut(s) 107, 340, 534, 1105, 1259, 2516, 2519, 2586, 3417
Tsp45I GTSAC 4 cut(s) 1631, 2252, 2270, 2876
TspGWI ACGGA 4 cut(s) 226, 2033, 2059, 2311
TspRI CASTG 6 cut(s) 90, 1185, 1594, 2562, 2847, 2973
Tth111I GACNNNGTC 1 cut(s) 185
Van91I CCANNNNNTGG 2 cut(s) 1472, 1512
Vha464I CTTAAG 1 cut(s) 538
VneI GTGCAC 2 cut(s) 1176, 2838
VpaK11BI GGWCC 5 cut(s) 432, 697, 729, 1597, 2550
VspI ATTAAT 1 cut(s) 288
XapI RAATTY 8 cut(s) 172, 386, 691, 763, 1192, 1668, 1734, 2604
XceI RCATGY 8 cut(s) 787, 1276, 1558, 2207, 2322, 2795, 2838, 2997
XcmI CCANNNNNNNNNTGG 2 cut(s) 82, 1607
XmnI GAANNNNTTC 2 cut(s) 272, 1695
XspI CTAG 4 cut(s) 465, 557, 1334, 1652
Zsp2I ATGCAT 3 cut(s) 1378, 2320, 3084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.