Rh4BG015600

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0007173
Physical Location & Seq
Forward (+)
2199275 .. 2207032
7758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG015600.1

Sequence Viewer

Length: 3438 bp
ATGCAAGTCCCCAATTCTGAAGAAACCGCCCCGGTAAACCCCCAAACCCCTAAAGACCCCGAAACCTCCGATTCCAGTTCGGCAGTTGATGATGACGACAGTTCCGCTGCCGACGACGAGTCGTCCGTTCTCGATGTCTCCGGCAAAAGCTTGGATTTTTCAATTGGGGAGAATTCCGGCGACGGCGTCGGCGTCGGGGCTTTGTATTTGTACAAAAATGTGTATAATTTATTGCCCAAGTCCGTCGGGAGGCTGAAGCGGCTGAGGACGTTGAAGTTTTTCGGGAATGAGATTAATTTGTTCTCTTCGTCGGAGTTTGGGAACTTGGTGGGATTGGAATGCTTGCAGCTCCGGCTATCGTCGCCGGCGTTTGATGGTCTGCCATTGCATAAATTTAAAGGCTTGAAGGAGCTGGAGCTTTCGAAAGTGCCTTCTCGGTCCTCGGCTATACCGATACTGAGTGAGATTGCTAGGCTCAACTGTCTCACCAAGCTCTCTGTTTGTTACTTTTCTATAAGATACCTTCCTCCTGAAATTGGCTGCTTAAGCAAGTTGGAATACCTAGATCTTTCATTCAATAAGATGAAGAGTTTGCCAGCTGAAATTAGTAATTTAAATGCCTTGGTATCGTTAAAAGTTGCCAATAATAAATTATCAGAACTACCTTTGGCTTTGTCCTCGTTGAAAATGTTGGAGAACTTGGACCTGTCGCACAACAGGTTGACATCATTGGGGTCCCTTGATCTTAGCTTGATGCGAAGCCTCCAGAATTTAAATCTTCAGCACAACATGCTTCCCATCTACTGTCAAATACCTTCATGGATATGTTGCAATTTGGAAGGTAATGGAAAAGATATGTCCAACGACGATTTTAGCAGTACCTCAGTTGAAATGGATGTATATGAAACCACCATTCAGAAGAATGATGAAAATCGTTTTCACACAGGCTTTCATCATAGCTCATCGAGTATAGTTGGGCCTTCATCTAACAGTAGGTGTTTCACAACTCGGAGATCAGCTGGCCGGTGGAAGCGACAGTTCTTGCAGAGGAGAGCTCGTCAAGAACGCTTAAATAACAGCAGGAAGTCAAAAGGTCTGGATCTTCCTAAGCTGCACATGAAGGAGGATGAAGACTGCAAACCAGGCAATATAGATGCTACTTTTGAATCATATCGTGAAAGTGCACTGGACGTTATAAATTTGGATGATGATAATGATAAAAGTTTACTTTCTGGAGAAGATGAAGGTGTAAACGTCTCCCATGCTGCACATCATGACATGTGTTCGAAGGAGGAGCTTTCTGTAAGAAGTTGCTCATCTCTCAGGGTTAACTCTACTCTAGTAGATAATGGTGATAAAAAGGATTGTTACGAGAGTGATGCATCGTCAACTTGCAATCAAGAGGTTACTGGTGAGCATGATGACGTGCCATATTCAGGAAAATCCAAATGCAGTTCAAAGTCCAAGAGGCCACGAGATGGGGATCTTGATAATAGGAATCTTCAGGATCCCAAACGTTGGAAGTGTGGAGATTGTAGTTCAAGCCTATCTTGCAGAAACATGTCTTGCAAATATAGTAATATGTCATTTTGCAGTGCTGAGGACCATCTTCCAGATGGCTTTTATGATGCAGGACGTGACAGGCCCTTCATGCCACTAGAGAGTTATGAGCAAATTTTCCATCTCGACTCACGTGAAGTTATTCTTGTGGACAGGCTAAGGGATAAAGAGTTGGATGGAATTTTGTGCTCTGCCCGAGATATGGTGTCTCAATTGAAGCAGTTAAATGGTTTAAATACAGATAGAGATCGGGATGATGAGCTACAGATTGCATTATATCTTGCTCTATTTGTATCAGATCATTTTGGAGGGACTGATAGGGCGGCTCTTGTTGAAAGGAGACGAAGAGCTGGTGGTGGTTCAATATCCAGGAAGCCTTTTGTATGCACCTGCTCAATTAGGAACTCTGAATCTATTAGTTTGAACCCCAAACAGTCTCCGGAGACTGTTGAAGATATTGCCTTTTCTGATATCTGTGAGAAATCTCTCCGTTCTATCAAAGCCAGACGAAAATCCGTTGTAGTACCTATAGGAACATTGCAGTTTGGTGTTTGTAGGCATAGAGCATTACTACTGAAGTACCTATGTGATCGGATGGAACCTCCAGTCCCTTGTGAGCTTGTCAGAGGATACCTGGATTTCATGCCACATGCTTGGAATATTATATCAGTCAAGAAAAATGACTCAGCCAAGAGAGGTGACTCAATTAAGGGAGGTGACTTGGTTGAGAGAGATGACTCACGGATTCGCATGGTTGTTGATGCATGTCGTCCGCATGATGTAAGAGAGGAGACAGATCCTGAATATTATTGCAGGTATATTCCTCTCAGTCGGGCCAAGGTTTCTTTCTCTGGAAGCAGCCCTGCTGATGTTTGTTTGTTTCCTTCATTGTCCTCTTCGGATGAAACTCAAACTGAATCCGGTAGTTCTCTTATTCGGTGTAAATATGGGTCGGCTGATGCAGCAGCAAAGATGCGTACTCTGGAGGTATATGGGACCTCAATGGATGATATTAGGAACTTTGAATACAGCTGCTTGGGTGAAGTTAGAATTTTAGGTGCTTTGCAACACCCCTGCATAGTAGAGATGTATGGACACAGTATATCATCCAAGTGGGCTCCTTCTATGGATGATAATTCTGGGCACCGTATATTGCAATCTGTAATTTTCATGGAGTATATAAACGGGGGTTCCTTAAAGGGTTACATAGAAAAGCTATCTAAAGCAGGTGAGAAGCATGTCCCTGTGGAGTTAGCTTTAAGCATTGCTAAGAATGTTGCATGTGCACTGGTGGAGTTGCACTCAAAACACATTATTCATCGTGACATAAAAAGTGCAAACATTTTGATTGATATCGATAGGAAGACAGCTGATGGAACACCTGTTGTGAAGCTCTGTGATTTTGATAGGGCAGTGCCTCTTAGCTCGTACTTGCATACATGCTGTATTGCCCATGTTGGGATACCTCCTCCTGATGTTTGTGTTGGAACACCTCGCTGGATGGCTCCAGAGGTTCTGCGGGCAATGCATAAACAAAATATTTATGGATTGGAAGTTGATATCTGGTCGTTTGGATGCCTTCTTTTGGAGATGTTGACTTTGCAGATTCCTTACTTGGGATTATCTGAATTAGAGATTCATGACTGCCTATCGATGGGAAAACGACCCAAGTTAACAGATAACTTGGAGGCACTCCGGCCATTGGAGAAGCCTGCAATGGCTCAATCTGCTGAAGAGCTTGAGCAAACAGAAGCTGACTTAGATGTACTAAGATTTCTGGTTGACTTGTTCTGCCAATGTACTGAGGAGAATCCGCAAAGTCGTCCAACGGCTGACAGCCTGTACAAATTGTTGCTCGAACGCAGCACCCAGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

1145

Amino Acids

127.75

Weight (kDa)

5.67

Isoelectric Point (pI)

52.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 122 - 261 2.2e-12 Leucine-rich repeat region
LRR_8 PF13855 162 - 218 5.9e-06 Leucine rich repeat
EDR1_CTR1_ARMC3_pept PF14381 677 - 744 4.4e-11 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 863 - 1135 9.6e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 864 - 1135 3.4e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1196, 3436
AarI CACCTGC 2 cut(s) 1958, 2777
Acc36I ACCTGC 3 cut(s) 1958, 2364, 2777
AccB1I GGYRCC 1 cut(s) 2703
AccB7I CCANNNNNTGG 2 cut(s) 1478, 1518
AccIII TCCGGA 1 cut(s) 1999
AciI CCGC 7 cut(s) 27, 105, 259, 1883, 2333, 3079, 3374
AclI AACGTT 1 cut(s) 1516
AclWI GGATC 5 cut(s) 1107, 1491, 1502, 1515, 2351
AcoI YGGCCR 2 cut(s) 1021, 3257
AcsI RAATTY 7 cut(s) 172, 392, 769, 1198, 1674, 1740, 2610
AcuI CTGAAG 6 cut(s) 39, 275, 764, 1487, 2156, 3312
AcvI CACGTG 1 cut(s) 1694
AcyI GRCGYC 2 cut(s) 186, 192
AfaI GTAC 9 cut(s) 212, 880, 2085, 2141, 2539, 2990, 3327, 3361, 3404
AflII CTTAAG 1 cut(s) 544
AflIII ACRYGT 2 cut(s) 1278, 1560
AhdI GACNNNNNGTC 2 cut(s) 118, 121
AjiI CACGTC 2 cut(s) 1426, 1637
AjnI CCWGG 3 cut(s) 1141, 1928, 2193
AjuI GAANNNNNNNTTGG 2 cut(s) 2390, 2422
AloI GAACNNNNNNTCC 2 cut(s) 2151, 2183
Alw21I GWGCWC 4 cut(s) 1057, 1186, 1751, 2848
Alw26I GTCTC 8 cut(s) 142, 488, 1261, 1773, 1894, 1997, 2001, 2345
Alw44I GTGCAC 2 cut(s) 1182, 2844
AlwI GGATC 5 cut(s) 1107, 1491, 1502, 1515, 2351
AlwNI CAGNNNCTG 2 cut(s) 2360, 3314
Ama87I CYCGRG 1 cut(s) 1755
Aor13HI TCCGGA 1 cut(s) 1999
AoxI GGCC 6 cut(s) 977, 1021, 1469, 1643, 2394, 3257
ApaLI GTGCAC 2 cut(s) 1182, 2844
ApoI RAATTY 7 cut(s) 172, 392, 769, 1198, 1674, 1740, 2610
AseI ATTAAT 1 cut(s) 294
Asp700I GAANNNNTTC 2 cut(s) 278, 1701
AspS9I GGNCC 8 cut(s) 438, 703, 735, 977, 1603, 1644, 2394, 2556
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 7 cut(s) 478, 1364, 1424, 2270, 2288, 2612, 2801
AsuII TTCGAA 2 cut(s) 422, 1286
AvaI CYCGRG 1 cut(s) 1755
AvaII GGWCC 5 cut(s) 438, 703, 735, 1603, 2556
BaeGI GKGCMC 3 cut(s) 1186, 2706, 2848
BaeI ACNNNNGTAYC 2 cut(s) 2123, 2156
BamHI GGATCC 1 cut(s) 1507
BanI GGYRCC 1 cut(s) 2703
BanII GRGCYC 2 cut(s) 1057, 2680
BauI CACGAG 1 cut(s) 1473
BbrPI CACGTG 1 cut(s) 1694
BbsI GAAGAC 2 cut(s) 1137, 2930
Bbv12I GWGCWC 4 cut(s) 1057, 1186, 1751, 2848
BbvCI CCTCAGC 2 cut(s) 263, 1599
BbvI GCAGC 9 cut(s) 94, 358, 527, 1098, 1252, 2430, 2534, 2537, 2579
BceAI ACGGC 2 cut(s) 199, 3405
BcgI CGANNNNNNTGC 2 cut(s) 1361, 1395
BciT130I CCWGG 3 cut(s) 1143, 1930, 2195
BciVI GTATCC 2 cut(s) 2183, 3015
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 8 cut(s) 142, 488, 1261, 1773, 1894, 1997, 2001, 2345
BfaI CTAG 4 cut(s) 471, 563, 1340, 1658
BfmI CTRYAG 2 cut(s) 1823, 2088
BfrI CTTAAG 1 cut(s) 544
BfuAI ACCTGC 3 cut(s) 1958, 2364, 2777
BfuI GTATCC 2 cut(s) 2183, 3015
BglII AGATCT 1 cut(s) 565
Bme1390I CCNGG 4 cut(s) 32, 1143, 1930, 2195
Bme18I GGWCC 5 cut(s) 438, 703, 735, 1603, 2556
BmeRI GACNNNNNGTC 2 cut(s) 118, 121
BmeT110I CYCGRG 1 cut(s) 1755
BmgBI CACGTC 2 cut(s) 1426, 1637
BmgT120I GGNCC 8 cut(s) 438, 703, 735, 977, 1603, 1644, 2394, 2556
BmiI GGNNCC 9 cut(s) 736, 737, 1509, 2160, 2557, 2679, 2705, 2752, 3066
BmrFI CCNGG 4 cut(s) 32, 1143, 1930, 2195
BmrI ACTGGG 1 cut(s) 3424
BmsI GCATC 9 cut(s) 744, 1144, 1369, 1391, 1618, 2311, 2509, 2523, 3125
BmuI ACTGGG 1 cut(s) 3424
BpiI GAAGAC 2 cut(s) 1137, 2930
BplI GAGNNNNNCTC 4 cut(s) 1039, 1071, 2846, 2878
BpmI CTGGAG 6 cut(s) 434, 749, 1254, 2148, 2564, 3051
Bpu10I CCTNAGC 4 cut(s) 263, 1107, 1599, 1718
Bpu14I TTCGAA 2 cut(s) 422, 1286
BpuEI CTTGAG 1 cut(s) 3320
BpuMI CCSGG 1 cut(s) 32
Bsa29I ATCGAT 2 cut(s) 2916, 3212
BsaAI YACGTR 1 cut(s) 1694
BsaBI GATNNNNATC 4 cut(s) 930, 1482, 1806, 2912
BsaHI GRCGYC 2 cut(s) 186, 192
BsaJI CCNNGG 4 cut(s) 30, 441, 621, 2397
BsaWI WCCGGW 2 cut(s) 1999, 2480
BsaXI ACNNNNNCTCC 2 cut(s) 1042, 1072
Bse118I RCCGGY 2 cut(s) 364, 1023
Bse1I ACTGG 6 cut(s) 75, 1191, 1414, 2165, 2853, 3430
Bse3DI GCAATG 5 cut(s) 383, 2096, 2823, 3090, 3282
Bse8I GATNNNNATC 4 cut(s) 930, 1482, 1806, 2912
BseAI TCCGGA 1 cut(s) 1999
BseBI CCWGG 3 cut(s) 1143, 1930, 2195
BseCI ATCGAT 2 cut(s) 2916, 3212
BseDI CCNNGG 4 cut(s) 30, 441, 621, 2397
BseJI GATNNNNATC 4 cut(s) 930, 1482, 1806, 2912
BseMI GCAATG 5 cut(s) 383, 2096, 2823, 3090, 3282
BseMII CTCAG 8 cut(s) 254, 449, 897, 1336, 1590, 2259, 2401, 3354
BseNI ACTGG 6 cut(s) 75, 1191, 1414, 2165, 2853, 3430
BseRI GAGGAG 5 cut(s) 1063, 1307, 2363, 3018, 3380
BseSI GKGCMC 3 cut(s) 1186, 2706, 2848
BseXI GCAGC 9 cut(s) 94, 358, 527, 1098, 1252, 2430, 2534, 2537, 2579
BsgI GTGCAG 2 cut(s) 1097, 1251
BshFI GGCC 6 cut(s) 979, 1023, 1471, 1645, 2396, 3259
BshNI GGYRCC 1 cut(s) 2703
BshVI ATCGAT 2 cut(s) 2916, 3212
BsiHKAI GWGCWC 4 cut(s) 1057, 1186, 1751, 2848
BsiHKCI CYCGRG 1 cut(s) 1755
BsiSI CCGG 9 cut(s) 32, 141, 177, 352, 365, 1024, 2000, 2481, 3256
BslFI GGGAC 5 cut(s) 721, 1885, 2153, 2569, 2786
BsmAI GTCTC 8 cut(s) 142, 488, 1261, 1773, 1894, 1997, 2001, 2345
BsmBI CGTCTC 2 cut(s) 1261, 1894
BsmFI GGGAC 5 cut(s) 721, 1885, 2153, 2569, 2786
BsmI GAATGC 1 cut(s) 344
BsnI GGCC 6 cut(s) 979, 1023, 1471, 1645, 2396, 3259
BsoBI CYCGRG 1 cut(s) 1755
Bsp119I TTCGAA 2 cut(s) 422, 1286
Bsp1286I GDGCHC 6 cut(s) 1057, 1186, 1751, 2680, 2706, 2848
Bsp13I TCCGGA 1 cut(s) 1999
Bsp1407I TGTACA 2 cut(s) 210, 3402
BspACI CCGC 7 cut(s) 27, 105, 259, 1883, 2333, 3079, 3374
BspANI GGCC 6 cut(s) 979, 1023, 1471, 1645, 2396, 3259
BspCNI CTCAG 8 cut(s) 255, 450, 896, 1335, 1591, 2258, 2400, 3355
BspDI ATCGAT 2 cut(s) 2916, 3212
BspEI TCCGGA 1 cut(s) 1999
BspHI TCATGA 2 cut(s) 1273, 3199
BspLI GGNNCC 9 cut(s) 736, 737, 1509, 2160, 2557, 2679, 2705, 2752, 3066
BspMI ACCTGC 3 cut(s) 1958, 2364, 2777
BspPI GGATC 5 cut(s) 1107, 1491, 1502, 1515, 2351
BspQI GCTCTTC 2 cut(s) 1900, 3288
BspT104I TTCGAA 2 cut(s) 422, 1286
BspT107I GGYRCC 1 cut(s) 2703
BspTI CTTAAG 1 cut(s) 544
BsrDI GCAATG 5 cut(s) 383, 2096, 2823, 3090, 3282
BsrFI RCCGGY 2 cut(s) 364, 1023
BsrGI TGTACA 2 cut(s) 210, 3402
BsrI ACTGG 6 cut(s) 75, 1191, 1414, 2165, 2853, 3430
BssAI RCCGGY 2 cut(s) 364, 1023
BssECI CCNNGG 4 cut(s) 30, 441, 621, 2397
BssNI GRCGYC 2 cut(s) 186, 192
BssSI CACGAG 1 cut(s) 1473
BssT1I CCWWGG 2 cut(s) 621, 2397
Bst2BI CACGAG 1 cut(s) 1473
Bst2UI CCWGG 3 cut(s) 1143, 1930, 2195
Bst4CI ACNGT 9 cut(s) 101, 482, 806, 992, 1038, 1995, 2008, 2660, 2708
Bst6I CTCTTC 5 cut(s) 310, 581, 1900, 2461, 3288
BstACI GRCGYC 2 cut(s) 186, 192
BstAFI CTTAAG 1 cut(s) 544
BstAPI GCANNNNNTGC 1 cut(s) 790
BstAUI TGTACA 2 cut(s) 210, 3402
BstBAI YACGTR 1 cut(s) 1694
BstBI TTCGAA 2 cut(s) 422, 1286
BstC8I GCNNGC 6 cut(s) 344, 366, 597, 1021, 3081, 3273
BstMAI GTCTC 8 cut(s) 142, 488, 1261, 1773, 1894, 1997, 2001, 2345
BstNI CCWGG 3 cut(s) 1143, 1930, 2195
BstNSI RCATGY 8 cut(s) 793, 1282, 1564, 2213, 2328, 2801, 2844, 3003
BstSCI CCNGG 4 cut(s) 30, 1141, 1928, 2193
BstSFI CTRYAG 2 cut(s) 1823, 2088
BstSLI GKGCMC 3 cut(s) 1186, 2706, 2848
BstV1I GCAGC 9 cut(s) 94, 358, 527, 1098, 1252, 2430, 2534, 2537, 2579
BstV2I GAAGAC 2 cut(s) 1137, 2930
BstX2I RGATCY 5 cut(s) 565, 1099, 1483, 1507, 2356
BstXI CCANNNNNNTGG 1 cut(s) 2214
BstYI RGATCY 5 cut(s) 565, 1099, 1483, 1507, 2356
Bsu15I ATCGAT 2 cut(s) 2916, 3212
BsuI GTATCC 2 cut(s) 2183, 3015
BsuRI GGCC 6 cut(s) 979, 1023, 1471, 1645, 2396, 3259
BsuTUI ATCGAT 2 cut(s) 2916, 3212
BtrI CACGTC 2 cut(s) 1426, 1637
BtsI GCAGTG 2 cut(s) 1600, 2979
BtsIMutI CAGTG 4 cut(s) 1184, 1600, 2846, 2979
BveI ACCTGC 3 cut(s) 1958, 2364, 2777
Cac8I GCNNGC 6 cut(s) 344, 366, 597, 1021, 3081, 3273
CaiI CAGNNNCTG 2 cut(s) 2360, 3314
CciI TCATGA 2 cut(s) 1273, 3199
Cfr10I RCCGGY 2 cut(s) 364, 1023
Cfr13I GGNCC 8 cut(s) 438, 703, 735, 977, 1603, 1644, 2394, 2556
ClaI ATCGAT 2 cut(s) 2916, 3212
CseI GACGC 2 cut(s) 175, 181
Csp6I GTAC 9 cut(s) 211, 879, 2084, 2140, 2538, 2989, 3326, 3360, 3403
CviQI GTAC 9 cut(s) 211, 879, 2084, 2140, 2538, 2989, 3326, 3360, 3403
DraI TTTAAA 4 cut(s) 397, 615, 774, 1794
DriI GACNNNNNGTC 2 cut(s) 118, 121
EaeI YGGCCR 2 cut(s) 1021, 3257
Eam1104I CTCTTC 5 cut(s) 310, 581, 1900, 2461, 3288
Eam1105I GACNNNNNGTC 2 cut(s) 118, 121
EarI CTCTTC 5 cut(s) 310, 581, 1900, 2461, 3288
Ecl136II GAGCTC 1 cut(s) 1055
Eco130I CCWWGG 2 cut(s) 621, 2397
Eco24I GRGCYC 2 cut(s) 1057, 2680
Eco32I GATATC 3 cut(s) 2032, 2914, 3121
Eco47I GGWCC 5 cut(s) 438, 703, 735, 1603, 2556
Eco53kI GAGCTC 1 cut(s) 1055
Eco57I CTGAAG 6 cut(s) 39, 275, 764, 1487, 2156, 3312
Eco72I CACGTG 1 cut(s) 1694
Eco88I CYCGRG 1 cut(s) 1755
EcoICRI GAGCTC 1 cut(s) 1055
EcoO109I RGGNCCY 3 cut(s) 735, 1644, 2556
EcoRI GAATTC 1 cut(s) 172
EcoRII CCWGG 3 cut(s) 1141, 1928, 2193
EcoRV GATATC 3 cut(s) 2032, 2914, 3121
EcoT14I CCWWGG 2 cut(s) 621, 2397
EcoT22I ATGCAT 3 cut(s) 1384, 2326, 3090
EcoT38I GRGCYC 2 cut(s) 1057, 2680
ErhI CCWWGG 2 cut(s) 621, 2397
Esp3I CGTCTC 2 cut(s) 1261, 1894
FalI AAGNNNNNCTT 4 cut(s) 1534, 1566, 1689, 1721
FaqI GGGAC 5 cut(s) 721, 1885, 2153, 2569, 2786
FauI CCCGC 1 cut(s) 3072
FriOI GRGCYC 2 cut(s) 1057, 2680
FspBI CTAG 4 cut(s) 471, 563, 1340, 1658
GsuI CTGGAG 6 cut(s) 434, 749, 1254, 2148, 2564, 3051
HaeIII GGCC 6 cut(s) 979, 1023, 1471, 1645, 2396, 3259
HapII CCGG 9 cut(s) 32, 141, 177, 352, 365, 1024, 2000, 2481, 3256
HgaI GACGC 2 cut(s) 175, 181
Hin1I GRCGYC 2 cut(s) 186, 192
HincII GTYRAC 6 cut(s) 723, 1330, 1389, 3156, 3234, 3343
HindII GTYRAC 6 cut(s) 723, 1330, 1389, 3156, 3234, 3343
HindIII AAGCTT 1 cut(s) 148
HpaI GTTAAC 2 cut(s) 1330, 3234
HpaII CCGG 9 cut(s) 32, 141, 177, 352, 365, 1024, 2000, 2481, 3256
HphI GGTGA 7 cut(s) 478, 1364, 1424, 2270, 2288, 2612, 2801
Hpy99I CGWCG 9 cut(s) 116, 119, 185, 191, 197, 248, 313, 364, 869
HpyCH4III ACNGT 9 cut(s) 101, 482, 806, 992, 1038, 1995, 2008, 2660, 2708
HpyCH4IV ACGT 7 cut(s) 269, 1191, 1254, 1425, 1516, 1636, 1693
HpySE526I ACGT 7 cut(s) 269, 1191, 1254, 1425, 1516, 1636, 1693
Hsp92I GRCGYC 2 cut(s) 186, 192
KflI GGGWCCC 1 cut(s) 735
Kpn2I TCCGGA 1 cut(s) 1999
KroI GCCGGC 1 cut(s) 364
KroNI GCCGGC 1 cut(s) 366
KspAI GTTAAC 2 cut(s) 1330, 3234
LguI GCTCTTC 2 cut(s) 1900, 3288
LmnI GCTCC 6 cut(s) 354, 409, 415, 1294, 2683, 3070
Lsp1109I GCAGC 9 cut(s) 94, 358, 527, 1098, 1252, 2430, 2534, 2537, 2579
LweI GCATC 9 cut(s) 744, 1144, 1369, 1391, 1618, 2311, 2509, 2523, 3125
MaeI CTAG 4 cut(s) 471, 563, 1340, 1658
MaeII ACGT 7 cut(s) 269, 1191, 1254, 1425, 1516, 1636, 1693
MaeIII GTNAC 8 cut(s) 503, 1367, 1405, 1637, 2258, 2276, 2762, 2882
MfeI CAATTG 2 cut(s) 162, 1772
MflI RGATCY 5 cut(s) 565, 1099, 1483, 1507, 2356
MhlI GDGCHC 6 cut(s) 1057, 1186, 1751, 2680, 2706, 2848
MlyI GAGTC 5 cut(s) 128, 1682, 2237, 2255, 2291
MmeI TCCRAC 8 cut(s) 291, 534, 672, 885, 1499, 1713, 3025, 3410
Mph1103I ATGCAT 3 cut(s) 1384, 2326, 3090
MreI CGCCGGCG 1 cut(s) 364
MroI TCCGGA 1 cut(s) 1999
MroNI GCCGGC 1 cut(s) 364
MroXI GAANNNNTTC 2 cut(s) 278, 1701
MslI CAYNNNNRTG 2 cut(s) 823, 2671
MspA1I CMGCKG 5 cut(s) 107, 599, 1019, 2592, 2930
MspCI CTTAAG 1 cut(s) 544
MspI CCGG 9 cut(s) 32, 141, 177, 352, 365, 1024, 2000, 2481, 3256
MspR9I CCNGG 4 cut(s) 32, 1143, 1930, 2195
MunI CAATTG 2 cut(s) 162, 1772
Mva1269I GAATGC 1 cut(s) 344
MvaI CCWGG 3 cut(s) 1143, 1930, 2195
NaeI GCCGGC 1 cut(s) 366
NciI CCSGG 1 cut(s) 32
NgoMIV GCCGGC 1 cut(s) 364
NlaIV GGNNCC 9 cut(s) 736, 737, 1509, 2160, 2557, 2679, 2705, 2752, 3066
NmeAIII GCCGAG 1 cut(s) 422
NmuCI GTSAC 4 cut(s) 1637, 2258, 2276, 2882
NsiI ATGCAT 3 cut(s) 1384, 2326, 3090
NspI RCATGY 8 cut(s) 793, 1282, 1564, 2213, 2328, 2801, 2844, 3003
NspV TTCGAA 2 cut(s) 422, 1286
PagI TCATGA 2 cut(s) 1273, 3199
PaqCI CACCTGC 2 cut(s) 1958, 2777
PciI ACATGT 2 cut(s) 1278, 1560
PciSI GCTCTTC 2 cut(s) 1900, 3288
PcsI WCGNNNNNNNCGW 4 cut(s) 123, 189, 365, 449
PctI GAATGC 1 cut(s) 344
PdiI GCCGGC 1 cut(s) 366
PdmI GAANNNNTTC 2 cut(s) 278, 1701
PfeI GAWTC 9 cut(s) 71, 1166, 1498, 1970, 2305, 2477, 3166, 3196, 3370
PflFI GACNNNGTC 1 cut(s) 185
PflMI CCANNNNNTGG 2 cut(s) 1478, 1518
PfoI TCCNGGA 1 cut(s) 1928
PleI GAGTC 5 cut(s) 127, 1682, 2237, 2255, 2291
PmaCI CACGTG 1 cut(s) 1694
PmlI CACGTG 1 cut(s) 1694
PpsI GAGTC 5 cut(s) 127, 1682, 2237, 2255, 2291
Ppu21I YACGTR 1 cut(s) 1694
PpuMI RGGWCCY 2 cut(s) 735, 2556
PscI ACATGT 2 cut(s) 1278, 1560
PshBI ATTAAT 1 cut(s) 294
PsiI TTATAA 2 cut(s) 1196, 3436
Psp124BI GAGCTC 1 cut(s) 1057
Psp1406I AACGTT 1 cut(s) 1516
Psp5II RGGWCCY 2 cut(s) 735, 2556
Psp6I CCWGG 3 cut(s) 1141, 1928, 2193
PspCI CACGTG 1 cut(s) 1694
PspGI CCWGG 3 cut(s) 1141, 1928, 2193
PspN4I GGNNCC 9 cut(s) 736, 737, 1509, 2160, 2557, 2679, 2705, 2752, 3066
PspPI GGNCC 8 cut(s) 438, 703, 735, 977, 1603, 1644, 2394, 2556
PspPPI RGGWCCY 2 cut(s) 735, 2556
PsrI GAACNNNNNNTAC 2 cut(s) 2570, 2602
PstNI CAGNNNCTG 2 cut(s) 2360, 3314
PsuI RGATCY 5 cut(s) 565, 1099, 1483, 1507, 2356
PsyI GACNNNGTC 1 cut(s) 185
PvuII CAGCTG 4 cut(s) 599, 1019, 2592, 2930
RsaI GTAC 9 cut(s) 212, 880, 2085, 2141, 2539, 2990, 3327, 3361, 3404
RsaNI GTAC 9 cut(s) 211, 879, 2084, 2140, 2538, 2989, 3326, 3360, 3403
RseI CAYNNNNRTG 2 cut(s) 823, 2671
SacI GAGCTC 1 cut(s) 1057
SapI GCTCTTC 2 cut(s) 1900, 3288
Sau96I GGNCC 8 cut(s) 438, 703, 735, 977, 1603, 1644, 2394, 2556
SchI GAGTC 5 cut(s) 128, 1682, 2237, 2255, 2291
ScrFI CCNGG 4 cut(s) 32, 1143, 1930, 2195
SduI GDGCHC 6 cut(s) 1057, 1186, 1751, 2680, 2706, 2848
SfaNI GCATC 9 cut(s) 744, 1144, 1369, 1391, 1618, 2311, 2509, 2523, 3125
SfcI CTRYAG 2 cut(s) 1823, 2088
SfuI TTCGAA 2 cut(s) 422, 1286
SgrAI CRCCGGYG 1 cut(s) 364
SinI GGWCC 5 cut(s) 438, 703, 735, 1603, 2556
SmiI ATTTAAAT 2 cut(s) 615, 774
SmiMI CAYNNNNRTG 2 cut(s) 823, 2671
SmlI CTYRAG 2 cut(s) 544, 3299
SmoI CTYRAG 2 cut(s) 544, 3299
SsiI CCGC 7 cut(s) 27, 105, 259, 1883, 2333, 3079, 3374
SspI AATATT 3 cut(s) 2221, 2366, 3100
SspMI CTAG 4 cut(s) 471, 563, 1340, 1658
SstI GAGCTC 1 cut(s) 1057
StyD4I CCNGG 4 cut(s) 30, 1141, 1928, 2193
StyI CCWWGG 2 cut(s) 621, 2397
SwaI ATTTAAAT 2 cut(s) 615, 774
TaaI ACNGT 9 cut(s) 101, 482, 806, 992, 1038, 1995, 2008, 2660, 2708
TaiI ACGT 7 cut(s) 272, 1194, 1257, 1428, 1519, 1639, 1696
TaqI TCGA 8 cut(s) 132, 422, 965, 1286, 1686, 2916, 3212, 3417
TaqII GACCGA 1 cut(s) 426
TatI WGTACW 4 cut(s) 210, 3325, 3359, 3402
TauI GCSGC 2 cut(s) 262, 1886
TfiI GAWTC 9 cut(s) 71, 1166, 1498, 1970, 2305, 2477, 3166, 3196, 3370
TscAI CASTG 4 cut(s) 1191, 1600, 2853, 2979
TseFI GTSAC 4 cut(s) 1637, 2258, 2276, 2882
Tsp45I GTSAC 4 cut(s) 1637, 2258, 2276, 2882
TspGWI ACGGA 5 cut(s) 115, 232, 2039, 2065, 2317
TspRI CASTG 4 cut(s) 1191, 1600, 2853, 2979
Tth111I GACNNNGTC 1 cut(s) 185
Van91I CCANNNNNTGG 2 cut(s) 1478, 1518
Vha464I CTTAAG 1 cut(s) 544
VneI GTGCAC 2 cut(s) 1182, 2844
VpaK11BI GGWCC 5 cut(s) 438, 703, 735, 1603, 2556
VspI ATTAAT 1 cut(s) 294
XapI RAATTY 7 cut(s) 172, 392, 769, 1198, 1674, 1740, 2610
XceI RCATGY 8 cut(s) 793, 1282, 1564, 2213, 2328, 2801, 2844, 3003
XcmI CCANNNNNNNNNTGG 1 cut(s) 1613
XmnI GAANNNNTTC 2 cut(s) 278, 1701
XspI CTAG 4 cut(s) 471, 563, 1340, 1658
Zsp2I ATGCAT 3 cut(s) 1384, 2326, 3090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.