MD16G1200600.v1.1

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007173
Physical Location & Seq
Forward (+)
18114100 .. 18120907
6808 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1200600.v1.1.491

Sequence Viewer

Length: 3462 bp
ATGCCATTATTCCTATATTTTCTCGCCTCCCAAACAGATCTCTCAGCCTGCCCGCTTCAGAAATTCGGGTTTTTTTTCCCCGGATTCCGGCTCCAATTTTCAATGCAAGTCCCCAATTCTGACGAAGCTGCCCCCGAAGGCACTAAGGTACCTGAGCAATCAGAAGCTGAAGCCTCCAATCCGACTGTGGAAGGTGAAGACTGCGGTGCCGCTGCCGACGGCGACGACGACCCTAGGGTTGTCGACATATCCGGCAAAAGCTTGGACTTTTCGATTAGGGAGAATTCTGAGGATGCCGGGGCTTTGTATTTGTACAAAAACGTGTTTAATTTATTGCCCAAGTCGATTGGCTCTCACAAGCGGTTGAGGACGCTGAAGTTCTTTGGGAATGAGATTAATTTATTTTCGCCGTCAGCGGCAATGGAGTTTGAGAGCTTGGAATGCTTGCAAGTGAGGATGTCGTCGCCGGAGTTTGGTGGGTTGCCGTTGGATAAATTGAGTGGCTTGAAGGAGCTTGAGCTTTCCAAAGTGCTGACGAGGCCTTCGGGGTTTCAGATATTGAGCGAGATTGCTCGGCTCAAGTGCCTCACCAAGCTCTCTGTTTGTCACTTCTCCATAAGATACCTCCCTCCAGAAATTGGCTGCTTAATCACTTTGGAATATCTAGATCTTTCATTCAACAAGATGAAGAATTTGCCTGCTGAAATCAGTAATTTAAATGCCTTGATATCGTTAAAAGTTGCTAATAATAAATTGGTGGAATTACCTTCGACTTTGTCCTCTTTGCAAAGGTTGGAGATCATGGACCTGTCAAATAATAGGTTGACATCACTAGGGTCTCTTGAACTTGACTTGATGCACAACCTCCAGATTTTAAATCTTCAGTACAACACGCTTCTCAATTTCTGTCAAATACCCTCATGGATATGTTGCAATTTGGAAGGAAGTGGGAAGGACACACACAGTGATGATGTTACCATTTCCCCTGTTGAAATGGATGTATATGAAACCCCCCTTCAGAAAAATAATGAAAGCCATTTTCGTAGGGGCTTTCATCATAACTCAGCAAGTCTAATTTTCACGCATTCATCAACCAGTAGGTGCTGTGCAACTCGGAGGTCAGGTAGGTGGAGGAAGCAAGGGTATAGGTTGCAGCTGAGAACTCGTCAAGAACGCTTAAACAACAGCAGGAAGTTGAAAGGTCTTGATCTTTCCAATCAGTTACACGTGAAGGAAGATGGAGAATGCAAACCAGGCAACCCTGATCTTCTTGCTTCTGAATCTTATCTTGAAGGTGCATCAGACATTATCAATCTGCATGATGATGGTGAGAAAGATTTACAATCTAGGGAAGTGCGAAGTGAAAATGTTGTGTGCTATGATATGAATTTGAAGGAGCATTTTGTAGGAAATTCCTCATCAGTGAGTCCAGACTCTAGTGCAGTAGATGAAAGTGATGAAAAGGATTGTTGTGAGTTTGATGCATTGTTGGCCCCCAATCAACGGGTTTATGGTGAGGATTATGAAGGTTCATCTTCAGATACATCAAAAAGTATTTGTCAACCCAAAAGGCACCTTGATGGCCACCTTGATAATCCAAGACGGCCCAAACATCCTAGATACAGTCCTAATAGTTCAAACTTGTCTCGGAAATATAATGACATCTCGGTTTGCAGCACTGAGGACCATCTTTCAGATGGCTTTTATGATGCAGGGCGTGACAGGCCATTCATGCCACTAGAACTTTATGATGAAAAGTTCCGTCTTGACTCACGTGAAGTCATTCTCGTGGACAGGCAATGGGATAAAGAGTTGGATGTAATTTCACGTTCTGCCCAAGAATTGGTGAATCGTTTAAATACAGATGGAAATAAGGCTGATATGCTGCAGATTGCATCATTGCTTGCTCTGTTTGTATCAGATCACTTTGGGGGGAGTGATAGGAGTGCTCTTGTTGAATGGTCACGAAAAGCTAATCCTCTTTCAGACTACAAAAAGCCTTTTGTTTGCACCTGCCCAACTGGAAACAAGGGCTTTATTAGTTTGCCCACTAAACCAGTTGTAAGGAATGTTGAAGATATCGTTTTTTCTGATGTCTCTGAGAAATCTCTTCATTCTATCAAAGCACGACGAAATTCCATTGTAGTTCCCATTGGAACCTTGCAGTTTGGTGTGTGTCGGCATAGAGCATTGCTATTGAAGTATCTTTGTGATTGGGTGGAGCCTCGAGTACCTTGTGAGCTTATTAGGGGTTATTTGGATTTCATGCCACATGCGTGGAATAATGTATTAATCAAGAGAGGTGCCAAAGAGATTCGAATGTTAGTTGATGCATGTCGTCCATGTGATATAAGAGCGGAGACGGATCCTGAATATTATTGCAGGTATATTCCTCTCAGTCGGACCAAAGTTTATCTTCCAATTCGAAACAGCCCTGCTCCTACCTCCTTCCCTTCTATGCCCTCTTGTGATGAAACTCTGAAAAACTCTGTTACTTCTCTCGTCCGGCGCAAATATGGATCAAATGAGGCAGCAGGAAAGATGCGTACTTCTGTGGTATATGGAACTCCAACGGATGAAATTAGGAACTTTGACTACAACTGCTTAGGGGAAATAAGGATTTTAGGTGCTTTGAAACACCCTTGCATAGTGGAAATGTATGGACATCAGATATCTTCCGAGTGGGTTCCTCCAGGAGATGGTAGTCGTGAGCATCGTATATTGCAGTCTATAATTTGGATGGAGGACATAAAAGGGGGTTCCTTACAGAATTATATAGAAAAGTTATCAAAAGCTGGTGAGAAGCATCTCCCAGTGGAGTTAGCTTTGCGCATTGCTAAAAATGTTGCATGTGCACTGGTGGAGCTGCATTCAAAGCACATTATTCACCGGGACATAAAAAGTGCAAACATATTGATTGATCTGGATAGAAAGAGAGCTGATGGAACACCTGTTGTGAAGCTCTGTGATTTTGATAGGGCGATTCCCCTTCGATCTTACTTGCATACATGCTGCATTGCCCACGTTGGAATACCTCCTGCTAATGTTTGTGCTGGAACACCTCGCTGGATGGCCCCAGAGGTTTTGCGAGCAGTGCATGAACAAAGTATTTATGGATTGGAAATTGATATCTGGTCATTTGGGTGCTTGCTGTTGGAAATGTTGACTTTGCAAATTCCGTACTTCGGAGTACCTGACTTGGAGATTCATGAACTTCTGACGAAGGGAAAACGACCAAATTTAACAGATGACTTGGAGGCGTTTCGGTCGTTGAATGAGCCAGTAATGACTCAAGCAGGCGCAGAACTTGATGGAACAGAGGCTGACTTCGACACACTGAGATTTCTTGTTGACTTGTTTTACCAATGTACGGAGGAGAATCCGGAGAATCGTCCTACGGCTGACAACCTCCACGAATTGATACTGGAGCACAGCAAAACTCATACAAACTCCGAAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

1154

Amino Acids

129.96

Weight (kDa)

5.72

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 187 - 297 1.8e-09 Leucine-rich repeat region
EDR1_CTR1_ARMC3_pept PF14381 546 - 665 4.6e-06 EDR1/CTR1/ARMC3, peptidase-like
EDR1_CTR1_ARMC3_pept PF14381 700 - 777 1.2e-11 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 866 - 1137 2.5e-33 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 867 - 1082 4.8e-23 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2021
Acc16I TGCGCA 1 cut(s) 2831
Acc36I ACCTGC 2 cut(s) 2021, 2373
Acc65I GGTACC 1 cut(s) 148
AccB1I GGYRCC 4 cut(s) 148, 206, 1572, 2303
AccB7I CCANNNNNTGG 3 cut(s) 638, 1843, 2699
AccBSI CCGCTC 1 cut(s) 2357
AccI GTMKAC 1 cut(s) 243
AccIII TCCGGA 1 cut(s) 3382
AciI CCGC 6 cut(s) 53, 204, 210, 361, 416, 2357
AclWI GGATC 3 cut(s) 2360, 2373, 2527
AcoI YGGCCR 1 cut(s) 1582
AcsI RAATTY 8 cut(s) 62, 283, 691, 1387, 1411, 2134, 3174, 3238
AcuI CTGAAG 6 cut(s) 41, 189, 395, 866, 1001, 1521
AcvI CACGTG 2 cut(s) 1228, 1775
AdeI CACNNNGTG 1 cut(s) 965
AfaI GTAC 8 cut(s) 150, 314, 887, 2232, 2548, 3182, 3192, 3370
AfiI CCNNNNNNNGG 8 cut(s) 87, 473, 638, 1503, 1843, 2699, 3185, 3370
AflIII ACRYGT 2 cut(s) 321, 1225
AjnI CCWGG 2 cut(s) 1252, 2692
AleI CACNNNNGTG 1 cut(s) 2275
Alw21I GWGCWC 3 cut(s) 1951, 2857, 3432
Alw26I GTCTC 4 cut(s) 843, 1650, 2101, 2354
Alw44I GTGCAC 1 cut(s) 2853
AlwI GGATC 3 cut(s) 2360, 2373, 2527
AlwNI CAGNNNCTG 2 cut(s) 167, 3323
Ama87I CYCGRG 1 cut(s) 2226
Aor13HI TCCGGA 1 cut(s) 3382
AoxI GGCC 6 cut(s) 539, 1491, 1582, 1604, 1724, 3072
ApaLI GTGCAC 1 cut(s) 2853
ApeKI GCWGC 9 cut(s) 128, 212, 642, 1153, 1674, 1885, 2531, 2866, 3012
ApoI RAATTY 8 cut(s) 62, 283, 691, 1387, 1411, 2134, 3174, 3238
ArsI GACNNNNNNTTYG 2 cut(s) 526, 558
AseI ATTAAT 2 cut(s) 396, 2291
Asp700I GAANNNNTTC 1 cut(s) 1782
Asp718I GGTACC 1 cut(s) 148
AspA2I CCTAGG 1 cut(s) 233
AspLEI GCGC 3 cut(s) 2511, 2832, 3302
AspS9I GGNCC 6 cut(s) 805, 1492, 1605, 1684, 2403, 3073
AsuC2I CCSGG 3 cut(s) 81, 298, 2891
AsuHPI GGTGA 7 cut(s) 206, 580, 1340, 1526, 1858, 2810, 2879
AsuII TTCGAA 2 cut(s) 2317, 2425
AvaI CYCGRG 1 cut(s) 2226
AvaII GGWCC 3 cut(s) 805, 1684, 2403
AvrII CCTAGG 1 cut(s) 233
BaeGI GKGCMC 1 cut(s) 2857
BaeI ACNNNNGTAYC 2 cut(s) 132, 165
BalI TGGCCA 1 cut(s) 1584
BamHI GGATCC 1 cut(s) 2365
BanI GGYRCC 4 cut(s) 148, 206, 1572, 2303
BauI CACGAG 1 cut(s) 1787
BbrPI CACGTG 2 cut(s) 1228, 1775
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 3 cut(s) 1951, 2857, 3432
BbvI GCAGC 9 cut(s) 115, 199, 629, 1165, 1686, 1872, 2543, 2853, 2999
BceAI ACGGC 5 cut(s) 235, 394, 469, 1619, 3414
BciT130I CCWGG 2 cut(s) 1254, 2694
BcnI CCSGG 3 cut(s) 81, 298, 2891
BcoDI GTCTC 4 cut(s) 843, 1650, 2101, 2354
BfaI CTAG 7 cut(s) 234, 665, 833, 1347, 1437, 1617, 1739
BfmI CTRYAG 1 cut(s) 1886
BfuAI ACCTGC 2 cut(s) 2021, 2373
BglII AGATCT 2 cut(s) 37, 667
BlnI CCTAGG 1 cut(s) 233
Bme1390I CCNGG 5 cut(s) 81, 298, 1254, 2694, 2891
Bme18I GGWCC 3 cut(s) 805, 1684, 2403
BmeT110I CYCGRG 1 cut(s) 2226
BmgT120I GGNCC 6 cut(s) 805, 1492, 1605, 1684, 2403, 3073
BmrFI CCNGG 5 cut(s) 81, 298, 1254, 2694, 2891
BmrI ACTGGG 1 cut(s) 2807
BmuI ACTGGG 1 cut(s) 2807
BpiI GAAGAC 1 cut(s) 204
BpmI CTGGAG 4 cut(s) 615, 851, 2676, 3446
Bpu10I CCTNAGC 2 cut(s) 153, 2605
Bpu14I TTCGAA 2 cut(s) 2317, 2425
BpuEI CTTGAG 3 cut(s) 536, 563, 3276
BpuMI CCSGG 3 cut(s) 81, 298, 2891
BsaAI YACGTR 2 cut(s) 1228, 1775
BsaI GGTCTC 1 cut(s) 843
BsaJI CCNNGG 3 cut(s) 79, 233, 297
BsaWI WCCGGW 1 cut(s) 3382
BsaXI ACNNNNNCTCC 2 cut(s) 1108, 1138
Bsc4I CCNNNNNNNGG 8 cut(s) 87, 473, 638, 1503, 1843, 2699, 3185, 3370
Bse1I ACTGG 7 cut(s) 1095, 2026, 2057, 2813, 2862, 3281, 3429
Bse3DI GCAATG 6 cut(s) 426, 1805, 1898, 2189, 2832, 3015
BseAI TCCGGA 1 cut(s) 3382
BseBI CCWGG 2 cut(s) 1254, 2694
BseDI CCNNGG 3 cut(s) 79, 233, 297
BseGI GGATG 8 cut(s) 298, 462, 1003, 1612, 1822, 2581, 2745, 3075
BseLI CCNNNNNNNGG 8 cut(s) 87, 473, 638, 1503, 1843, 2699, 3185, 3370
BseMI GCAATG 6 cut(s) 426, 1805, 1898, 2189, 2832, 3015
BseMII CTCAG 9 cut(s) 57, 144, 279, 1077, 1148, 1671, 2091, 2410, 3329
BseNI ACTGG 7 cut(s) 1095, 2026, 2057, 2813, 2862, 3281, 3429
BseRI GAGGAG 1 cut(s) 3389
BseSI GKGCMC 1 cut(s) 2857
BseXI GCAGC 9 cut(s) 115, 199, 629, 1165, 1686, 1872, 2543, 2853, 2999
BsgI GTGCAG 1 cut(s) 1461
Bsh1285I CGRYCG 1 cut(s) 3269
BshFI GGCC 6 cut(s) 541, 1493, 1584, 1606, 1726, 3074
BshNI GGYRCC 4 cut(s) 148, 206, 1572, 2303
BsiEI CGRYCG 1 cut(s) 3269
BsiHKAI GWGCWC 3 cut(s) 1951, 2857, 3432
BsiHKCI CYCGRG 1 cut(s) 2226
BsiSI CCGG 8 cut(s) 81, 88, 252, 297, 467, 2506, 2890, 3383
BslFI GGGAC 2 cut(s) 95, 2906
BslI CCNNNNNNNGG 8 cut(s) 87, 473, 638, 1503, 1843, 2699, 3185, 3370
BsmAI GTCTC 4 cut(s) 843, 1650, 2101, 2354
BsmBI CGTCTC 1 cut(s) 2354
BsmFI GGGAC 2 cut(s) 95, 2906
BsmI GAATGC 4 cut(s) 446, 1084, 1250, 2869
BsnI GGCC 6 cut(s) 541, 1493, 1584, 1606, 1726, 3074
Bso31I GGTCTC 1 cut(s) 843
BsoBI CYCGRG 1 cut(s) 2226
Bsp119I TTCGAA 2 cut(s) 2317, 2425
Bsp1286I GDGCHC 3 cut(s) 1951, 2857, 3432
Bsp13I TCCGGA 1 cut(s) 3382
Bsp1407I TGTACA 1 cut(s) 312
BspACI CCGC 6 cut(s) 53, 204, 210, 361, 416, 2357
BspANI GGCC 6 cut(s) 541, 1493, 1584, 1606, 1726, 3074
BspCNI CTCAG 9 cut(s) 56, 145, 280, 1076, 1149, 1672, 2092, 2409, 3330
BspEI TCCGGA 1 cut(s) 3382
BspHI TCATGA 1 cut(s) 3208
BspMAI CTGCAG 1 cut(s) 1890
BspMI ACCTGC 2 cut(s) 2021, 2373
BspPI GGATC 3 cut(s) 2360, 2373, 2527
BspT104I TTCGAA 2 cut(s) 2317, 2425
BspT107I GGYRCC 4 cut(s) 148, 206, 1572, 2303
BspTNI GGTCTC 1 cut(s) 843
BsrBI CCGCTC 1 cut(s) 2357
BsrDI GCAATG 6 cut(s) 426, 1805, 1898, 2189, 2832, 3015
BsrGI TGTACA 1 cut(s) 312
BsrI ACTGG 7 cut(s) 1095, 2026, 2057, 2813, 2862, 3281, 3429
BssECI CCNNGG 3 cut(s) 79, 233, 297
BssSI CACGAG 1 cut(s) 1787
BssT1I CCWWGG 1 cut(s) 233
Bst2BI CACGAG 1 cut(s) 1787
Bst2UI CCWGG 2 cut(s) 1254, 2694
Bst4CI ACNGT 3 cut(s) 187, 965, 1625
Bst6I CTCTTC 1 cut(s) 2115
BstAUI TGTACA 1 cut(s) 312
BstBAI YACGTR 2 cut(s) 1228, 1775
BstBI TTCGAA 2 cut(s) 2317, 2425
BstC8I GCNNGC 8 cut(s) 49, 53, 446, 699, 1905, 3090, 3149, 3298
BstF5I GGATG 8 cut(s) 298, 462, 1003, 1612, 1822, 2581, 2745, 3075
BstHHI GCGC 3 cut(s) 2511, 2832, 3302
BstMAI GTCTC 4 cut(s) 843, 1650, 2101, 2354
BstMCI CGRYCG 1 cut(s) 3269
BstMWI GCNNNNNNNGC 8 cut(s) 441, 538, 1254, 1490, 1723, 1732, 2875, 3094
BstNI CCWGG 2 cut(s) 1254, 2694
BstNSI RCATGY 4 cut(s) 2276, 2337, 2853, 3012
BstSCI CCNGG 5 cut(s) 79, 296, 1252, 2692, 2889
BstSFI CTRYAG 1 cut(s) 1886
BstSLI GKGCMC 1 cut(s) 2857
BstV1I GCAGC 9 cut(s) 115, 199, 629, 1165, 1686, 1872, 2543, 2853, 2999
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 3 cut(s) 37, 667, 2365
BstXI CCANNNNNNTGG 1 cut(s) 2277
BstYI RGATCY 3 cut(s) 37, 667, 2365
BsuRI GGCC 6 cut(s) 541, 1493, 1584, 1606, 1726, 3074
BtsCI GGATG 8 cut(s) 298, 462, 1003, 1612, 1822, 2581, 2745, 3075
BtsI GCAGTG 1 cut(s) 3099
BtsIMutI CAGTG 7 cut(s) 970, 1428, 1677, 2820, 2855, 3099, 3335
BveI ACCTGC 2 cut(s) 2021, 2373
Cac8I GCNNGC 8 cut(s) 49, 53, 446, 699, 1905, 3090, 3149, 3298
CaiI CAGNNNCTG 2 cut(s) 167, 3323
CciI TCATGA 1 cut(s) 3208
CfoI GCGC 3 cut(s) 2511, 2832, 3302
Cfr13I GGNCC 6 cut(s) 805, 1492, 1605, 1684, 2403, 3073
CseI GACGC 1 cut(s) 379
Csp6I GTAC 8 cut(s) 149, 313, 886, 2231, 2547, 3181, 3191, 3369
CviQI GTAC 8 cut(s) 149, 313, 886, 2231, 2547, 3181, 3191, 3369
DraI TTTAAA 3 cut(s) 717, 876, 1857
DraIII CACNNNGTG 1 cut(s) 965
EaeI YGGCCR 1 cut(s) 1582
Eam1104I CTCTTC 1 cut(s) 2115
EarI CTCTTC 1 cut(s) 2115
Eco130I CCWWGG 1 cut(s) 233
Eco147I AGGCCT 1 cut(s) 541
Eco31I GGTCTC 1 cut(s) 843
Eco32I GATATC 4 cut(s) 729, 2080, 2673, 3130
Eco47I GGWCC 3 cut(s) 805, 1684, 2403
Eco57I CTGAAG 6 cut(s) 41, 189, 395, 866, 1001, 1521
Eco72I CACGTG 2 cut(s) 1228, 1775
Eco88I CYCGRG 1 cut(s) 2226
EcoRI GAATTC 1 cut(s) 283
EcoRII CCWGG 2 cut(s) 1252, 2692
EcoRV GATATC 4 cut(s) 729, 2080, 2673, 3130
EcoT14I CCWWGG 1 cut(s) 233
EcoT22I ATGCAT 2 cut(s) 1486, 2335
ErhI CCWWGG 1 cut(s) 233
Esp3I CGTCTC 1 cut(s) 2354
FalI AAGNNNNNCTT 2 cut(s) 2400, 2432
FaqI GGGAC 2 cut(s) 95, 2906
FauI CCCGC 1 cut(s) 60
FblI GTMKAC 1 cut(s) 243
FokI GGATG 8 cut(s) 305, 469, 1010, 1599, 1829, 2588, 2752, 3082
FspBI CTAG 7 cut(s) 234, 665, 833, 1347, 1437, 1617, 1739
FspI TGCGCA 1 cut(s) 2831
GlaI GCGC 3 cut(s) 2510, 2831, 3301
GsuI CTGGAG 4 cut(s) 615, 851, 2676, 3446
HaeIII GGCC 6 cut(s) 541, 1493, 1584, 1606, 1726, 3074
HapII CCGG 8 cut(s) 81, 88, 252, 297, 467, 2506, 2890, 3383
HgaI GACGC 1 cut(s) 379
HhaI GCGC 3 cut(s) 2511, 2832, 3302
Hin6I GCGC 3 cut(s) 2509, 2830, 3300
HinP1I GCGC 3 cut(s) 2509, 2830, 3300
HincII GTYRAC 5 cut(s) 244, 825, 1562, 3165, 3352
HindII GTYRAC 5 cut(s) 244, 825, 1562, 3165, 3352
HindIII AAGCTT 1 cut(s) 259
HpaII CCGG 8 cut(s) 81, 88, 252, 297, 467, 2506, 2890, 3383
HphI GGTGA 7 cut(s) 206, 580, 1340, 1526, 1858, 2810, 2879
Hpy166II GTNNAC 7 cut(s) 244, 825, 1562, 1792, 2855, 3165, 3352
Hpy8I GTNNAC 7 cut(s) 244, 825, 1562, 1792, 2855, 3165, 3352
Hpy99I CGWCG 5 cut(s) 221, 227, 230, 466, 2133
HpyCH4III ACNGT 3 cut(s) 187, 965, 1625
HpyCH4IV ACGT 5 cut(s) 321, 1227, 1774, 1828, 3024
HpyF10VI GCNNNNNNNGC 8 cut(s) 441, 538, 1254, 1490, 1723, 1732, 2875, 3094
HpySE526I ACGT 5 cut(s) 321, 1227, 1774, 1828, 3024
HspAI GCGC 3 cut(s) 2509, 2830, 3300
Kpn2I TCCGGA 1 cut(s) 3382
KpnI GGTACC 1 cut(s) 152
LmnI GCTCC 7 cut(s) 96, 511, 1396, 2221, 2443, 2863, 3427
Lsp1109I GCAGC 9 cut(s) 115, 199, 629, 1165, 1686, 1872, 2543, 2853, 2999
MaeI CTAG 7 cut(s) 234, 665, 833, 1347, 1437, 1617, 1739
MaeII ACGT 5 cut(s) 321, 1227, 1774, 1828, 3024
MaeIII GTNAC 6 cut(s) 605, 973, 1221, 1718, 1962, 2491
MbiI CCGCTC 1 cut(s) 2357
MflI RGATCY 3 cut(s) 37, 667, 2365
MhlI GDGCHC 3 cut(s) 1951, 2857, 3432
MlsI TGGCCA 1 cut(s) 1584
MluNI TGGCCA 1 cut(s) 1584
MlyI GAGTC 4 cut(s) 1427, 1435, 1763, 3283
MmeI TCCRAC 8 cut(s) 206, 468, 774, 1794, 2381, 2594, 3007, 3135
Mox20I TGGCCA 1 cut(s) 1584
Mph1103I ATGCAT 2 cut(s) 1486, 2335
MroI TCCGGA 1 cut(s) 3382
MroXI GAANNNNTTC 1 cut(s) 1782
MscI TGGCCA 1 cut(s) 1584
MslI CAYNNNNRTG 7 cut(s) 925, 966, 1323, 1578, 1787, 2275, 3142
Msp20I TGGCCA 1 cut(s) 1584
MspA1I CMGCKG 3 cut(s) 212, 416, 1156
MspI CCGG 8 cut(s) 81, 88, 252, 297, 467, 2506, 2890, 3383
MspR9I CCNGG 5 cut(s) 81, 298, 1254, 2694, 2891
Mva1269I GAATGC 4 cut(s) 446, 1084, 1250, 2869
MvaI CCWGG 2 cut(s) 1254, 2694
MwoI GCNNNNNNNGC 8 cut(s) 441, 538, 1254, 1490, 1723, 1732, 2875, 3094
NciI CCSGG 3 cut(s) 81, 298, 2891
NmeAIII GCCGAG 1 cut(s) 553
NmuCI GTSAC 3 cut(s) 605, 1718, 1962
NsbI TGCGCA 1 cut(s) 2831
NsiI ATGCAT 2 cut(s) 1486, 2335
NspI RCATGY 4 cut(s) 2276, 2337, 2853, 3012
NspV TTCGAA 2 cut(s) 2317, 2425
OliI CACNNNNGTG 1 cut(s) 2275
PaeR7I CTCGAG 1 cut(s) 2226
PagI TCATGA 1 cut(s) 3208
PaqCI CACCTGC 1 cut(s) 2021
PceI AGGCCT 1 cut(s) 541
PcsI WCGNNNNNNNCGW 2 cut(s) 225, 2713
PctI GAATGC 4 cut(s) 446, 1084, 1250, 2869
PdmI GAANNNNTTC 1 cut(s) 1782
PfeI GAWTC 8 cut(s) 84, 1280, 1849, 2314, 2983, 3205, 3379, 3388
PflFI GACNNNGTC 1 cut(s) 775
PflMI CCANNNNNTGG 3 cut(s) 638, 1843, 2699
PfoI TCCNGGA 1 cut(s) 2692
PleI GAGTC 4 cut(s) 1427, 1434, 1763, 3283
PmaCI CACGTG 2 cut(s) 1228, 1775
PmlI CACGTG 2 cut(s) 1228, 1775
PpsI GAGTC 4 cut(s) 1427, 1434, 1763, 3283
Ppu21I YACGTR 2 cut(s) 1228, 1775
PshBI ATTAAT 2 cut(s) 396, 2291
Psp6I CCWGG 2 cut(s) 1252, 2692
PspCI CACGTG 2 cut(s) 1228, 1775
PspGI CCWGG 2 cut(s) 1252, 2692
PspPI GGNCC 6 cut(s) 805, 1492, 1605, 1684, 2403, 3073
PspXI VCTCGAGB 1 cut(s) 2226
PsrI GAACNNNNNNTAC 2 cut(s) 2579, 2611
PstI CTGCAG 1 cut(s) 1890
PstNI CAGNNNCTG 2 cut(s) 167, 3323
PsuI RGATCY 3 cut(s) 37, 667, 2365
PsyI GACNNNGTC 1 cut(s) 775
PvuII CAGCTG 1 cut(s) 1156
RsaI GTAC 8 cut(s) 150, 314, 887, 2232, 2548, 3182, 3192, 3370
RsaNI GTAC 8 cut(s) 149, 313, 886, 2231, 2547, 3181, 3191, 3369
RseI CAYNNNNRTG 7 cut(s) 925, 966, 1323, 1578, 1787, 2275, 3142
SalI GTCGAC 1 cut(s) 242
Sau96I GGNCC 6 cut(s) 805, 1492, 1605, 1684, 2403, 3073
SchI GAGTC 4 cut(s) 1427, 1435, 1763, 3283
ScrFI CCNGG 5 cut(s) 81, 298, 1254, 2694, 2891
SduI GDGCHC 3 cut(s) 1951, 2857, 3432
SfcI CTRYAG 1 cut(s) 1886
Sfr274I CTCGAG 1 cut(s) 2226
SfuI TTCGAA 2 cut(s) 2317, 2425
SinI GGWCC 3 cut(s) 805, 1684, 2403
SlaI CTCGAG 1 cut(s) 2226
SmiI ATTTAAAT 1 cut(s) 717
SmiMI CAYNNNNRTG 7 cut(s) 925, 966, 1323, 1578, 1787, 2275, 3142
SmlI CTYRAG 4 cut(s) 515, 578, 2226, 3291
SmoI CTYRAG 4 cut(s) 515, 578, 2226, 3291
SseBI AGGCCT 1 cut(s) 541
SsiI CCGC 6 cut(s) 53, 204, 210, 361, 416, 2357
SspI AATATT 1 cut(s) 2375
SspMI CTAG 7 cut(s) 234, 665, 833, 1347, 1437, 1617, 1739
StuI AGGCCT 1 cut(s) 541
StyD4I CCNGG 5 cut(s) 79, 296, 1252, 2692, 2889
StyI CCWWGG 1 cut(s) 233
SwaI ATTTAAAT 1 cut(s) 717
TaaI ACNGT 3 cut(s) 187, 965, 1625
TaiI ACGT 5 cut(s) 324, 1230, 1777, 1831, 3027
TaqI TCGA 9 cut(s) 243, 272, 344, 770, 2227, 2317, 2425, 2992, 3330
TaqII GACCGA 1 cut(s) 3255
TatI WGTACW 2 cut(s) 312, 885
TauI GCSGC 2 cut(s) 212, 419
TfiI GAWTC 8 cut(s) 84, 1280, 1849, 2314, 2983, 3205, 3379, 3388
TscAI CASTG 7 cut(s) 970, 1428, 1684, 2820, 2862, 3099, 3342
TseFI GTSAC 3 cut(s) 605, 1718, 1962
TseI GCWGC 9 cut(s) 128, 212, 642, 1153, 1674, 1885, 2531, 2866, 3012
Tsp45I GTSAC 3 cut(s) 605, 1718, 1962
TspGWI ACGGA 5 cut(s) 1751, 2378, 2588, 3168, 3386
TspRI CASTG 7 cut(s) 970, 1428, 1684, 2820, 2862, 3099, 3342
Tth111I GACNNNGTC 1 cut(s) 775
Van91I CCANNNNNTGG 3 cut(s) 638, 1843, 2699
VneI GTGCAC 1 cut(s) 2853
VpaK11BI GGWCC 3 cut(s) 805, 1684, 2403
VspI ATTAAT 2 cut(s) 396, 2291
XapI RAATTY 8 cut(s) 62, 283, 691, 1387, 1411, 2134, 3174, 3238
XbaI TCTAGA 1 cut(s) 664
XceI RCATGY 4 cut(s) 2276, 2337, 2853, 3012
XcmI CCANNNNNNNNNTGG 2 cut(s) 184, 1694
XhoI CTCGAG 1 cut(s) 2226
XmaJI CCTAGG 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 1782
XspI CTAG 7 cut(s) 234, 665, 833, 1347, 1437, 1617, 1739
Zsp2I ATGCAT 2 cut(s) 1486, 2335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.