Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0012718

Physical Location & Seq

Genomic Coordinates Reverse (-)

18112658 .. 18115094

Gene Structure 2437 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1201800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 453 bp

ATGGCAGACGTACTAAGCGAAGAACAGATTGTTGAGTTTAAAGAAGCATTTTGTATATTTGACAAGGATGGAGATGGTTGCATTACTGTGGATGAACTGGAGACGGTGATTCGGTCTCTGGATCAGAATCCGACGGAGAAGGAGCTTCAGGACATGATAAGCAAAGTGGACGTTGATGGAAATGGGACCATTGAGTTCGCAGAGTTCTTGAGCTTAATGGCAAACAAAATGAAGGAAACTGATGCAGAGGAGGAGCTCAAAGAGGCCTTCAAAGTATTTGACAAGGATCAGAATGGATACATTTCAGCTACCGAGTTGAGGCATGTAATGATCAATCTTGGCGAAAAATTGACCGACGAAGAGGTCGAGCAGATGATAAATGAGGCGGATTTGGACGGTGACGGACAAGTGAATTACGATGAATTCGTGAAGATGATGATGACCATTGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005513 GO:0007154 GO:0007165 GO:0008150 GO:0009593 GO:0009987 GO:0010035 GO:0010038 GO:0019722 GO:0019932 GO:0023052 GO:0035556 GO:0042221 GO:0050789 GO:0050794 GO:0050896 GO:0051592 GO:0051606 GO:0051716 GO:0065007

KEGG Pathways

Metabolic & Signaling

ko04014 ko04015 ko04016 ko04020 ko04022 ko04024 ko04070 ko04114 ko04218 ko04261 ko04270 ko04371 ko04626 ko04713 ko04720 ko04722 ko04728 ko04740 ko04744 ko04745 ko04750 ko04910 ko04912 ko04915 ko04916 ko04921 ko04922 ko04924 ko04925 ko04970 ko04971 ko05010 ko05031 ko05034 ko05133 ko05152 ko05167 ko05200 ko05214 ko05418 map04014 map04015 map04016 map04020 map04022 map04024 map04070 map04114 map04218 map04261 map04270 map04371 map04626 map04713 map04720 map04722 map04728 map04740 map04744 map04745 map04750 map04910 map04912 map04915 map04916 map04921 map04922 map04924 map04925 map04970 map04971 map05010 map05031 map05034 map05133 map05152 map05167 map05200 map05214 map05418

Pfam Domains

Protein Families

EF-hand_1 EF-hand_7

Protein Analysis

151

Amino Acids

17.02

Weight (kDa)

4.05

Isoelectric Point (pI)

31.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AIF-1	PF21008	7 - 76	3.8e-06	Allograft inflammatory factor 1
EF-hand_1	PF00036	12 - 40	3.6e-08	EF hand domain
EF-hand_6	PF13405	12 - 40	2.2e-08	EF-hand domain
EF-hand_7	PF13499	13 - 73	5.5e-17	EF-hand domain pair
EF-hand_9	PF14658	16 - 75	9.3e-08	EF-hand domain
EF-hand_8	PF13833	25 - 74	7e-09	EF-hand domain pair
EF-hand_1	PF00036	48 - 74	1.2e-07	EF hand domain
EF-hand_7	PF13499	83 - 147	4.9e-22	EF-hand domain pair
AIF-1	PF21008	84 - 147	6.3e-06	Allograft inflammatory factor 1
EF-hand_1	PF00036	85 - 112	7.2e-09	EF hand domain
EF-hand_6	PF13405	85 - 114	3.4e-09	EF-hand domain
EF-hand_5	PF13202	86 - 110	3.5e-06	EF hand
EF-hand_9	PF14658	88 - 148	3e-06	EF-hand domain
EF-hand_8	PF13833	98 - 146	4.8e-11	EF-hand domain pair
EF-hand_1	PF00036	121 - 148	6.6e-09	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0012718)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22930
fragaria_vesca	FvH4_4g01760 FvH4_4g01760
malus_domestica	MD13G1201800.v1.1 MD16G1202100.v1.1
prunus_persica	Prupe.1G023600_v2.0.a1 Prupe.1G023600_v2.0.a1
pyrus_communis	pycom13g17520 pycom16g17050
rosa_chinensis	RchiOBHm_Chr4g0388611
rosa_laevigata	RLG00000010034
rosa_multiflora	Rmu_sc0004459.1_g000014
rosa_roxburghii	Rroxscaffold_5G00335070
rosa_rugosa	Rorug03G0319300 Rorug03G0319400
rosa_samantha	Rh4BG016900 Rh4DG017700
rosa_wichuraiana	Rw4G001690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	362
AciI	CCGC	1 cut(s)	386
AclWI	GGATC	2 cut(s)	129, 294
AcsI	RAATTY	1 cut(s)	422
AcuI	CTGAAG	1 cut(s)	131
AfaI	GTAC	1 cut(s)	12
AfiI	CCNNNNNNNGG	1 cut(s)	318
AgsI	TTSAA	1 cut(s)	271
AluBI	AGCT	4 cut(s)	145, 213, 256, 308
AluI	AGCT	4 cut(s)	145, 213, 256, 308
Alw21I	GWGCWC	1 cut(s)	258
Alw26I	GTCTC	2 cut(s)	95, 120
AlwI	GGATC	2 cut(s)	129, 294
AoxI	GGCC	1 cut(s)	264
ApoI	RAATTY	1 cut(s)	422
AspS9I	GGNCC	1 cut(s)	186
AsuHPI	GGTGA	2 cut(s)	118, 410
AvaII	GGWCC	1 cut(s)	186
BanII	GRGCYC	1 cut(s)	258
Bbv12I	GWGCWC	1 cut(s)	258
BccI	CCATC	3 cut(s)	62, 68, 170
BciVI	GTATCC	1 cut(s)	290
BclI	TGATCA	1 cut(s)	330
BcoDI	GTCTC	2 cut(s)	95, 120
BfuI	GTATCC	1 cut(s)	290
Bme18I	GGWCC	1 cut(s)	186
BmgT120I	GGNCC	1 cut(s)	186
BmiI	GGNNCC	1 cut(s)	187
BmsI	GCATC	1 cut(s)	232
BpmI	CTGGAG	1 cut(s)	119
BpuEI	CTTGAG	1 cut(s)	229
BsaBI	GATNNNNATC	1 cut(s)	126
BsaI	GGTCTC	1 cut(s)	120
Bsc4I	CCNNNNNNNGG	1 cut(s)	318
Bse1I	ACTGG	1 cut(s)	102
Bse8I	GATNNNNATC	1 cut(s)	126
BseGI	GGATG	2 cut(s)	73, 97
BseJI	GATNNNNATC	1 cut(s)	126
BseLI	CCNNNNNNNGG	1 cut(s)	318
BseNI	ACTGG	1 cut(s)	102
BseRI	GAGGAG	2 cut(s)	263, 266
BshFI	GGCC	1 cut(s)	266
BsiHKAI	GWGCWC	1 cut(s)	258
BslFI	GGGAC	1 cut(s)	199
BslI	CCNNNNNNNGG	1 cut(s)	318
BsmAI	GTCTC	2 cut(s)	95, 120
BsmBI	CGTCTC	1 cut(s)	95
BsmFI	GGGAC	1 cut(s)	199
BsnI	GGCC	1 cut(s)	266
Bso31I	GGTCTC	1 cut(s)	120
Bsp1286I	GDGCHC	1 cut(s)	258
Bsp143I	GATC	3 cut(s)	121, 286, 330
BspACI	CCGC	1 cut(s)	386
BspANI	GGCC	1 cut(s)	266
BspLI	GGNNCC	1 cut(s)	187
BspPI	GGATC	2 cut(s)	129, 294
BspTNI	GGTCTC	1 cut(s)	120
BsrI	ACTGG	1 cut(s)	102
BssMI	GATC	3 cut(s)	121, 286, 330
Bst4CI	ACNGT	3 cut(s)	88, 106, 398
Bst6I	CTCTTC	1 cut(s)	354
BstDEI	CTNAG	1 cut(s)	14
BstF5I	GGATG	2 cut(s)	73, 97
BstKTI	GATC	3 cut(s)	124, 289, 333
BstMAI	GTCTC	2 cut(s)	95, 120
BstMBI	GATC	3 cut(s)	121, 286, 330
BstNSI	RCATGY	1 cut(s)	326
BsuI	GTATCC	1 cut(s)	290
BsuRI	GGCC	1 cut(s)	266
BtsCI	GGATG	2 cut(s)	73, 97
Cfr13I	GGNCC	1 cut(s)	186
Csp6I	GTAC	1 cut(s)	11
CviAII	CATG	2 cut(s)	154, 323
CviJI	RGCY	5 cut(s)	145, 213, 256, 266, 308
CviKI_1	RGCY	5 cut(s)	145, 213, 256, 266, 308
CviQI	GTAC	1 cut(s)	11
DdeI	CTNAG	1 cut(s)	14
DpnI	GATC	3 cut(s)	123, 288, 332
DpnII	GATC	3 cut(s)	121, 286, 330
DraI	TTTAAA	1 cut(s)	40
DrdI	GACNNNNNNGTC	1 cut(s)	362
DseDI	GACNNNNNNGTC	1 cut(s)	362
Eam1104I	CTCTTC	1 cut(s)	354
EarI	CTCTTC	1 cut(s)	354
EciI	GGCGGA	1 cut(s)	401
Ecl136II	GAGCTC	1 cut(s)	256
Eco147I	AGGCCT	1 cut(s)	266
Eco24I	GRGCYC	1 cut(s)	258
Eco31I	GGTCTC	1 cut(s)	120
Eco47I	GGWCC	1 cut(s)	186
Eco53kI	GAGCTC	1 cut(s)	256
Eco57I	CTGAAG	1 cut(s)	131
EcoICRI	GAGCTC	1 cut(s)	256
EcoRI	GAATTC	1 cut(s)	422
EcoT38I	GRGCYC	1 cut(s)	258
Esp3I	CGTCTC	1 cut(s)	95
FaeI	CATG	2 cut(s)	157, 326
FaiI	YATR	3 cut(s)	56, 155, 324
FaqI	GGGAC	1 cut(s)	199
FatI	CATG	2 cut(s)	153, 322
FbaI	TGATCA	1 cut(s)	330
FokI	GGATG	2 cut(s)	80, 104
FriOI	GRGCYC	1 cut(s)	258
GsuI	CTGGAG	1 cut(s)	119
HaeIII	GGCC	1 cut(s)	266
Hin1II	CATG	2 cut(s)	157, 326
HinfI	GANTC	2 cut(s)	109, 127
HphI	GGTGA	2 cut(s)	118, 410
Hpy166II	GTNNAC	1 cut(s)	169
Hpy188I	TCNGA	3 cut(s)	126, 132, 291
Hpy188III	TCNNGA	4 cut(s)	119, 149, 208, 427
Hpy8I	GTNNAC	1 cut(s)	169
Hpy99I	CGWCG	2 cut(s)	136, 359
HpyAV	CCTTC	3 cut(s)	133, 226, 277
HpyCH4III	ACNGT	3 cut(s)	88, 106, 398
HpyCH4IV	ACGT	2 cut(s)	9, 171
HpyCH4V	TGCA	2 cut(s)	81, 245
HpyF3I	CTNAG	1 cut(s)	14
HpySE526I	ACGT	2 cut(s)	9, 171
Hsp92II	CATG	2 cut(s)	157, 326
Ksp22I	TGATCA	1 cut(s)	330
Kzo9I	GATC	3 cut(s)	121, 286, 330
LmnI	GCTCC	2 cut(s)	142, 253
LpnPI	CCDG	3 cut(s)	83, 104, 134
LweI	GCATC	1 cut(s)	232
MaeII	ACGT	2 cut(s)	9, 171
MaeIII	GTNAC	1 cut(s)	398
MalI	GATC	3 cut(s)	123, 288, 332
MboI	GATC	3 cut(s)	121, 286, 330
MboII	GAAGA	3 cut(s)	32, 371, 442
MhlI	GDGCHC	1 cut(s)	258
MluCI	AATT	3 cut(s)	347, 412, 422
MmeI	TCCRAC	1 cut(s)	155
MnlI	CCTC	6 cut(s)	241, 244, 256, 312, 355, 376
MseI	TTAA	2 cut(s)	39, 215
MslI	CAYNNNNRTG	1 cut(s)	86
NdeII	GATC	3 cut(s)	121, 286, 330
NlaIII	CATG	2 cut(s)	157, 326
NlaIV	GGNNCC	1 cut(s)	187
NmuCI	GTSAC	1 cut(s)	398
NspI	RCATGY	1 cut(s)	326
PceI	AGGCCT	1 cut(s)	266
PcsI	WCGNNNNNNNCGW	3 cut(s)	15, 363, 423
PfeI	GAWTC	2 cut(s)	109, 127
Psp124BI	GAGCTC	1 cut(s)	258
PspN4I	GGNNCC	1 cut(s)	187
PspPI	GGNCC	1 cut(s)	186
RsaI	GTAC	1 cut(s)	12
RsaNI	GTAC	1 cut(s)	11
RseI	CAYNNNNRTG	1 cut(s)	86
SacI	GAGCTC	1 cut(s)	258
SaqAI	TTAA	2 cut(s)	39, 215
Sau3AI	GATC	3 cut(s)	121, 286, 330
Sau96I	GGNCC	1 cut(s)	186
SduI	GDGCHC	1 cut(s)	258
SetI	ASST	7 cut(s)	12, 147, 174, 215, 258, 310, 366
SfaNI	GCATC	1 cut(s)	232
SinI	GGWCC	1 cut(s)	186
SmiMI	CAYNNNNRTG	1 cut(s)	86
SmlI	CTYRAG	1 cut(s)	208
SmoI	CTYRAG	1 cut(s)	208
Sse9I	AATT	3 cut(s)	347, 412, 422
SseBI	AGGCCT	1 cut(s)	266
SsiI	CCGC	1 cut(s)	386
SstI	GAGCTC	1 cut(s)	258
StuI	AGGCCT	1 cut(s)	266
TaaI	ACNGT	3 cut(s)	88, 106, 398
TaiI	ACGT	2 cut(s)	12, 174
TaqI	TCGA	1 cut(s)	366
TaqII	GACCGA	2 cut(s)	102, 368
TasI	AATT	3 cut(s)	347, 412, 422
TfiI	GAWTC	2 cut(s)	109, 127
Tru1I	TTAA	2 cut(s)	39, 215
Tru9I	TTAA	2 cut(s)	39, 215
TseFI	GTSAC	1 cut(s)	398
Tsp45I	GTSAC	1 cut(s)	398
TspDTI	ATGAA	3 cut(s)	108, 245, 435
TspGWI	ACGGA	2 cut(s)	149, 417
VpaK11BI	GGWCC	1 cut(s)	186
XapI	RAATTY	1 cut(s)	422
XceI	RCATGY	1 cut(s)	326

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.