Rh4BG016900

Calmodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0012718
Physical Location & Seq
Reverse (-)
2703357 .. 2706293
2937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG016900.1

Sequence Viewer

Length: 483 bp
ATGGATCCGCGAACGAATTTGCGAAACCAAACATCTGAGGTACTAAGTGAAGAACAGATTGTTGAATTTAAAGAGGCATTTTGTCTATTTGACAAGGATGGAGACGGTTGCATAACTGTCGAAGAATTGGCGACAGTTATCCGGTCTCTGGATCAAAATCCAACAGAGGAGGAACTTCAGGATATGATTAGTGAAGTTGATGCTGATGGAAATGGGACCATAGAGTTTGCAGAGTTCTTGAACTTGATGGCAAACAAAATGAAGGAAACGGATGCAGAGGAGGAGCTTAAAGAGGCCTTCAAGGTATTCGACAAGGATCAGAATGGATATATATCAGCTAATGAGCTGAGGCATGTAATGATCAATCTAGGAGAAAAACTGACGGACGAAGAGGTCGAGCAGATGATCAAGGAGGCCGATTTGGATGGCGATGGTCAAGTTAACTATGACGAATTTGTGAAGATGATGATGACCATTGGATGA

Protein Analysis

160

Amino Acids

18.23

Weight (kDa)

4.06

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIF-1 PF21008 17 - 86 2.5e-07 Allograft inflammatory factor 1
EF-hand_1 PF00036 22 - 50 1.9e-08 EF hand domain
EF-hand_6 PF13405 22 - 50 3.7e-08 EF-hand domain
EF-hand_7 PF13499 23 - 83 3.3e-17 EF-hand domain pair
EF-hand_9 PF14658 26 - 85 8.7e-09 EF-hand domain
EF-hand_8 PF13833 35 - 84 5.4e-11 EF-hand domain pair
EF-hand_1 PF00036 58 - 84 1.4e-07 EF hand domain
EF-hand_7 PF13499 93 - 157 3.1e-22 EF-hand domain pair
EF-hand_1 PF00036 95 - 122 3.2e-09 EF hand domain
EF-hand_6 PF13405 95 - 124 1.9e-09 EF-hand domain
EF-hand_5 PF13202 96 - 120 3.5e-06 EF hand
EF-hand_9 PF14658 98 - 158 2.2e-06 EF-hand domain
EF-hand_8 PF13833 115 - 156 9.9e-09 EF-hand domain pair
EF-hand_1 PF00036 131 - 158 4.5e-09 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012718)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22930
fragaria_vesca FvH4_4g01760 FvH4_4g01760
malus_domestica MD13G1201800.v1.1 MD16G1202100.v1.1
prunus_persica Prupe.1G023600_v2.0.a1 Prupe.1G023600_v2.0.a1
pyrus_communis pycom13g17520 pycom16g17050
rosa_chinensis RchiOBHm_Chr4g0388611
rosa_laevigata RLG00000010034
rosa_multiflora Rmu_sc0004459.1_g000014
rosa_roxburghii Rroxscaffold_5G00335070
rosa_rugosa Rorug03G0319300 Rorug03G0319400
rosa_samantha Rh4BG016900 Rh4DG017700
rosa_wichuraiana Rw4G001690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 392
AccII CGCG 1 cut(s) 10
AciI CCGC 1 cut(s) 8
AclWI GGATC 3 cut(s) 12, 159, 324
AcsI RAATTY 3 cut(s) 16, 65, 452
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 1 cut(s) 42
AfiI CCNNNNNNNGG 1 cut(s) 148
AgsI TTSAA 3 cut(s) 65, 241, 301
AluBI AGCT 3 cut(s) 286, 338, 346
AluI AGCT 3 cut(s) 286, 338, 346
Alw26I GTCTC 2 cut(s) 96, 150
AlwI GGATC 3 cut(s) 12, 159, 324
AoxI GGCC 2 cut(s) 294, 414
ApoI RAATTY 3 cut(s) 16, 65, 452
AspS9I GGNCC 1 cut(s) 216
AvaII GGWCC 1 cut(s) 216
BamHI GGATCC 1 cut(s) 4
BbvCI CCTCAGC 1 cut(s) 347
BccI CCATC 5 cut(s) 92, 200, 241, 419, 425
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BclI TGATCA 2 cut(s) 360, 405
BcoDI GTCTC 2 cut(s) 96, 150
BfaI CTAG 1 cut(s) 368
Bme18I GGWCC 1 cut(s) 216
BmgT120I GGNCC 1 cut(s) 216
BmiI GGNNCC 2 cut(s) 6, 217
BmsI GCATC 2 cut(s) 190, 262
Bpu10I CCTNAGC 1 cut(s) 347
BsaBI GATNNNNATC 2 cut(s) 156, 331
BsaI GGTCTC 1 cut(s) 150
BsaWI WCCGGW 1 cut(s) 141
Bsc4I CCNNNNNNNGG 1 cut(s) 148
Bse8I GATNNNNATC 2 cut(s) 156, 331
BseGI GGATG 3 cut(s) 103, 277, 430
BseJI GATNNNNATC 2 cut(s) 156, 331
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMII CTCAG 2 cut(s) 27, 338
BseRI GAGGAG 3 cut(s) 182, 293, 296
Bsh1236I CGCG 1 cut(s) 10
BshFI GGCC 2 cut(s) 296, 416
BsiSI CCGG 1 cut(s) 142
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 1 cut(s) 148
BsmAI GTCTC 2 cut(s) 96, 150
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 1 cut(s) 229
BsnI GGCC 2 cut(s) 296, 416
Bso31I GGTCTC 1 cut(s) 150
Bsp143I GATC 5 cut(s) 4, 151, 316, 360, 405
BspACI CCGC 1 cut(s) 8
BspANI GGCC 2 cut(s) 296, 416
BspCNI CTCAG 2 cut(s) 28, 339
BspFNI CGCG 1 cut(s) 10
BspLI GGNNCC 2 cut(s) 6, 217
BspPI GGATC 3 cut(s) 12, 159, 324
BspTNI GGTCTC 1 cut(s) 150
BssMI GATC 5 cut(s) 4, 151, 316, 360, 405
Bst4CI ACNGT 3 cut(s) 107, 118, 136
Bst6I CTCTTC 1 cut(s) 384
BstDEI CTNAG 3 cut(s) 36, 44, 347
BstF5I GGATG 3 cut(s) 103, 277, 430
BstFNI CGCG 1 cut(s) 10
BstKTI GATC 5 cut(s) 7, 154, 319, 363, 408
BstMAI GTCTC 2 cut(s) 96, 150
BstMBI GATC 5 cut(s) 4, 151, 316, 360, 405
BstNSI RCATGY 1 cut(s) 356
BstUI CGCG 1 cut(s) 10
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 2 cut(s) 296, 416
BtgZI GCGATG 1 cut(s) 444
BtsCI GGATG 3 cut(s) 103, 277, 430
Cfr13I GGNCC 1 cut(s) 216
Csp6I GTAC 1 cut(s) 41
CviAII CATG 1 cut(s) 353
CviJI RGCY 5 cut(s) 286, 296, 338, 346, 416
CviKI_1 RGCY 5 cut(s) 286, 296, 338, 346, 416
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 3 cut(s) 36, 44, 347
DpnI GATC 5 cut(s) 6, 153, 318, 362, 407
DpnII GATC 5 cut(s) 4, 151, 316, 360, 405
DraI TTTAAA 1 cut(s) 70
DrdI GACNNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 392
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
Eco147I AGGCCT 1 cut(s) 296
Eco31I GGTCTC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 161
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 1 cut(s) 356
FaiI YATR 7 cut(s) 113, 185, 221, 330, 332, 354, 447
FaqI GGGAC 1 cut(s) 229
FatI CATG 1 cut(s) 352
FbaI TGATCA 2 cut(s) 360, 405
FokI GGATG 3 cut(s) 110, 284, 437
FspBI CTAG 1 cut(s) 368
HaeIII GGCC 2 cut(s) 296, 416
HapII CCGG 1 cut(s) 142
Hin1II CATG 1 cut(s) 356
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HpaI GTTAAC 1 cut(s) 442
HpaII CCGG 1 cut(s) 142
Hpy166II GTNNAC 1 cut(s) 442
Hpy188I TCNGA 2 cut(s) 37, 321
Hpy188III TCNNGA 3 cut(s) 149, 179, 238
Hpy8I GTNNAC 1 cut(s) 442
HpyAV CCTTC 2 cut(s) 256, 307
HpyCH4III ACNGT 3 cut(s) 107, 118, 136
HpyCH4V TGCA 3 cut(s) 111, 230, 275
HpyF3I CTNAG 3 cut(s) 36, 44, 347
Hsp92II CATG 1 cut(s) 356
Ksp22I TGATCA 2 cut(s) 360, 405
KspAI GTTAAC 1 cut(s) 442
Kzo9I GATC 5 cut(s) 4, 151, 316, 360, 405
LmnI GCTCC 1 cut(s) 283
LpnPI CCDG 3 cut(s) 134, 155, 164
LweI GCATC 2 cut(s) 190, 262
MaeI CTAG 1 cut(s) 368
MalI GATC 5 cut(s) 6, 153, 318, 362, 407
MboI GATC 5 cut(s) 4, 151, 316, 360, 405
MboII GAAGA 4 cut(s) 62, 134, 401, 472
MflI RGATCY 1 cut(s) 4
MluCI AATT 4 cut(s) 16, 65, 125, 452
MmeI TCCRAC 1 cut(s) 185
MseI TTAA 3 cut(s) 69, 288, 441
MspI CCGG 1 cut(s) 142
MvnI CGCG 1 cut(s) 10
NdeII GATC 5 cut(s) 4, 151, 316, 360, 405
NlaIII CATG 1 cut(s) 356
NlaIV GGNNCC 2 cut(s) 6, 217
NspI RCATGY 1 cut(s) 356
PceI AGGCCT 1 cut(s) 296
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PspN4I GGNNCC 2 cut(s) 6, 217
PspPI GGNCC 1 cut(s) 216
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
SaqAI TTAA 3 cut(s) 69, 288, 441
Sau3AI GATC 5 cut(s) 4, 151, 316, 360, 405
Sau96I GGNCC 1 cut(s) 216
SetI ASST 6 cut(s) 42, 288, 306, 340, 348, 396
SfaNI GCATC 2 cut(s) 190, 262
SinI GGWCC 1 cut(s) 216
Sse9I AATT 4 cut(s) 16, 65, 125, 452
SseBI AGGCCT 1 cut(s) 296
SsiI CCGC 1 cut(s) 8
SspMI CTAG 1 cut(s) 368
StuI AGGCCT 1 cut(s) 296
TaaI ACNGT 3 cut(s) 107, 118, 136
TaqI TCGA 3 cut(s) 120, 309, 396
TasI AATT 4 cut(s) 16, 65, 125, 452
Tru1I TTAA 3 cut(s) 69, 288, 441
Tru9I TTAA 3 cut(s) 69, 288, 441
TspDTI ATGAA 1 cut(s) 275
TspGWI ACGGA 2 cut(s) 284, 398
VpaK11BI GGWCC 1 cut(s) 216
XapI RAATTY 3 cut(s) 16, 65, 452
XceI RCATGY 1 cut(s) 356
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.