Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0011917

Physical Location & Seq

Genomic Coordinates Reverse (-)

19064588 .. 19068959

Gene Structure 4372 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1207900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1428 bp

ATGTCTCTGAATATCCCTAGCTCTACAATTCTTCTGCTCCTCCTCCTCCTCTGCTCCTTCACTGCAATCTCTGCTCACGAAAATGAAGACGCAACAATCAAAACAATGGAGGATTTCTCTGGCTACCCATTTCACGAATCACACTCTACCTCTCAGTCTCACACCACTGTTTCTTTACTCTCAGTTGAGACTCAAAGTTTGCAGAAACAGATTGATGAACTTTCGACTTATTCGGATACACCTGCCCCATCAGTTACCAGGGTCTTGTATACTGAGAAAGATGTATTGGCTCGTAGGTTCATCAAAAACTTGATGGGACTTTCTGGTCTATCTGTTAGAGAGGATGCTGTTGGTAACATATTTGGTCGGTGGGAAGGCTATAATCCAGATCTTTCTGCAGTTGCGACAGGTTCTCACATTGATGCCATTCCATACTCTGGAAAGTATGATGGTGTTGTGGGTGTTCTAGGTGCTATAGAAGCCATCAATGTACTGAAAAGGTCTGGCTTCAAACCCAAAAGATCACTGGAAGTTATCTTGTTCACCTCGGAAGAGCCTACGCGCTTTGGAATCAGCTGTCTAGGAAGCCGCTTAATGGCAGGGATTGAGGCCCTGGCTAATGCTCTGAAGACAACAGTTGATGGTCAAAATGTATCCTTCTTAGATGCTGCAGGATCTGCTGGCTACACTAAACATGAAGATGCTTTATTGAGTGTGTTCTTAAAAAAAGAAAGCTACTCTGGTTTTGTGGAATTGCATATTGAGCAAGGCCCCATTTTGGAGGAGGAAGGTATATCAATTGGTATTGTAACTGCCATTGCAGCCCCAGCAAGTATAAAAGTGGAGTTTGAAGGCAATGGAGGCCATGCCGGTGCTGTCCTAATGCCAAATAGAAATGATGCTGGACTAGCAGCTGCAGAATTAGCACTAGCTGTTGAGAAACACGTATTGGAATCTGGGTCTGTTGATACTGTCGGTACAGTTGGTATTCTGGAGCTACATCCTGGAGCCATTAACAGCATCCCAAGTAAATCGCATCTGGAAATTGACACAAGAGATATTGATGAAGAGCGAAGAAACATTGTAATTGAGAAAATTCATCGATCTGCAATTGCTATAGCAAGTAAACGTGGGGTCAGTCTATCAGAGTTCAAAATCATAAATCAGGATCCGCCAGCAATCTCTGATAAATCAATAATTGAGGCAATGGAAGCTTCATCGAAAGAGCTAAACTTAACGTACAAGTTCATGATTAGCCGAGCTTACCACGATTCATTGTTCATGGCCAGGGTTTCTCCGATGGGTATGATATTCATTCCATGTTACAAAGGTTACAGCCATAAGCCTGAAGAATATGCATCCATTCAGGATATATCTAACGGGGTCAGGGTATTAGCATTGACCCTAGCAAAGCTGTCATTGCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000255 GO:0000256 GO:0003674 GO:0003824 GO:0004848 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006139 GO:0006144 GO:0006145 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009112 GO:0009987 GO:0010135 GO:0010136 GO:0012505 GO:0016787 GO:0016810 GO:0016813 GO:0017144 GO:0019439 GO:0034641 GO:0042737 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043605 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046113 GO:0046483 GO:0046700 GO:0055086 GO:0071704 GO:0072521 GO:0072523 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00230 ko01100 ko01120 map00230 map01100 map01120

Pfam Domains

Protein Families

M20_dimer Peptidase_M20 Peptidase_M28

Protein Analysis

476

Amino Acids

51.28

Weight (kDa)

5.38

Isoelectric Point (pI)

44.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M28	PF04389	119 - 203	4.3e-08	Peptidase family M28
Peptidase_M20	PF01546	136 - 470	1.2e-26	Peptidase family M20/M25/M40

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011917)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43600
fragaria_vesca	FvH4_4g02810
malus_domestica	MD13G1207900.v1.1
prunus_persica	Prupe.1G031400_v2.0.a1
pyrus_communis	pycom13g18000
rosa_chinensis	RchiOBHm_Chr4g0390621 RchiOBHm_Chr4g0390681
rosa_laevigata	RLG00000009913
rosa_multiflora	Rmu_co8283127.1_g000001 Rmu_sc0000171.1_g000034 Rmu_sc0001380.1_g000018
rosa_roxburghii	Rroxscaffold_1G00049440 Rroxscaffold_5G00336590
rosa_rugosa	Rorug03G0331300 Rorug03G0331400
rosa_samantha	Rh4AG032900 Rh4BG026800 Rh4CG036700 Rh4DG029900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	250
AasI	GACNNNNNNGTC	1 cut(s)	324
Acc36I	ACCTGC	1 cut(s)	250
AccB7I	CCANNNNNTGG	1 cut(s)	437
AccI	GTMKAC	1 cut(s)	269
AccII	CGCG	1 cut(s)	562
AciI	CCGC	2 cut(s)	589, 1172
AclWI	GGATC	3 cut(s)	682, 1163, 1176
AcoI	YGGCCR	1 cut(s)	1284
AcsI	RAATTY	1 cut(s)	1095
AcuI	CTGAAG	2 cut(s)	647, 1368
AfaI	GTAC	3 cut(s)	492, 979, 1241
AfiI	CCNNNNNNNGG	3 cut(s)	437, 595, 778
AflIII	ACRYGT	1 cut(s)	943
AgsI	TTSAA	3 cut(s)	511, 851, 1153
AjnI	CCWGG	4 cut(s)	257, 612, 1003, 1286
AjuI	GAANNNNNNNTTGG	4 cut(s)	269, 301, 932, 964
Alw26I	GTCTC	3 cut(s)	9, 162, 182
AlwI	GGATC	3 cut(s)	682, 1163, 1176
AlwNI	CAGNNNCTG	1 cut(s)	677
AoxI	GGCC	4 cut(s)	609, 769, 862, 1284
ApeKI	GCWGC	4 cut(s)	668, 821, 911, 914
ApoI	RAATTY	1 cut(s)	1095
ArsI	GACNNNNNNTTYG	2 cut(s)	181, 213
AspLEI	GCGC	1 cut(s)	564
AspS9I	GGNCC	2 cut(s)	610, 770
AsuHPI	GGTGA	1 cut(s)	535
BalI	TGGCCA	1 cut(s)	1286
BamHI	GGATCC	1 cut(s)	1168
BbsI	GAAGAC	2 cut(s)	93, 635
BbvI	GCAGC	4 cut(s)	655, 833, 901, 923
BccI	CCATC	6 cut(s)	256, 307, 443, 491, 635, 1294
BciT130I	CCWGG	4 cut(s)	259, 614, 1005, 1288
BciVI	GTATCC	2 cut(s)	229, 664
BcoDI	GTCTC	3 cut(s)	9, 162, 182
BfaI	CTAG	6 cut(s)	18, 467, 581, 908, 929, 1406
BfmI	CTRYAG	5 cut(s)	396, 474, 669, 915, 1116
BfuAI	ACCTGC	1 cut(s)	250
BfuI	GTATCC	2 cut(s)	229, 664
BglII	AGATCT	1 cut(s)	388
BisI	GCNGC	5 cut(s)	589, 669, 822, 912, 915
BlsI	GCNGC	5 cut(s)	590, 670, 823, 913, 916
Bme1390I	CCNGG	4 cut(s)	259, 614, 1005, 1288
BmgT120I	GGNCC	2 cut(s)	610, 770
BmiI	GGNNCC	3 cut(s)	772, 1009, 1170
BmrFI	CCNGG	4 cut(s)	259, 614, 1005, 1288
BmsI	GCATC	8 cut(s)	334, 412, 655, 691, 889, 1029, 1045, 1367
BpiI	GAAGAC	2 cut(s)	93, 635
BplI	GAGNNNNNCTC	2 cut(s)	101, 133
BpmI	CTGGAG	2 cut(s)	1013, 1026
Bsa29I	ATCGAT	1 cut(s)	1102
BsaAI	YACGTR	1 cut(s)	946
BsaJI	CCNNGG	4 cut(s)	258, 546, 612, 1287
Bsc4I	CCNNNNNNNGG	3 cut(s)	437, 595, 778
Bse118I	RCCGGY	1 cut(s)	869
Bse1I	ACTGG	1 cut(s)	531
Bse3DI	GCAATG	4 cut(s)	816, 862, 1212, 1418
BseBI	CCWGG	4 cut(s)	259, 614, 1005, 1288
BseCI	ATCGAT	1 cut(s)	1102
BseDI	CCNNGG	4 cut(s)	258, 546, 612, 1287
BseGI	GGATG	4 cut(s)	349, 1000, 1020, 1358
BseLI	CCNNNNNNNGG	3 cut(s)	437, 595, 778
BseMI	GCAATG	4 cut(s)	816, 862, 1212, 1418
BseMII	CTCAG	3 cut(s)	167, 195, 264
BseNI	ACTGG	1 cut(s)	531
BseRI	GAGGAG	5 cut(s)	29, 32, 35, 38, 797
BseXI	GCAGC	4 cut(s)	655, 833, 901, 923
BseYI	CCCAGC	1 cut(s)	826
Bsh1236I	CGCG	1 cut(s)	562
BshFI	GGCC	4 cut(s)	611, 771, 864, 1286
BshVI	ATCGAT	1 cut(s)	1102
BsiSI	CCGG	1 cut(s)	870
BslFI	GGGAC	1 cut(s)	330
BslI	CCNNNNNNNGG	3 cut(s)	437, 595, 778
BsmAI	GTCTC	3 cut(s)	9, 162, 182
BsmFI	GGGAC	1 cut(s)	330
BsnI	GGCC	4 cut(s)	611, 771, 864, 1286
Bsp143I	GATC	5 cut(s)	388, 521, 674, 1103, 1168
BspACI	CCGC	2 cut(s)	589, 1172
BspANI	GGCC	4 cut(s)	611, 771, 864, 1286
BspCNI	CTCAG	3 cut(s)	166, 194, 265
BspDI	ATCGAT	1 cut(s)	1102
BspFNI	CGCG	1 cut(s)	562
BspHI	TCATGA	1 cut(s)	1248
BspLI	GGNNCC	3 cut(s)	772, 1009, 1170
BspMAI	CTGCAG	3 cut(s)	400, 673, 919
BspMI	ACCTGC	1 cut(s)	250
BspPI	GGATC	3 cut(s)	682, 1163, 1176
BspQI	GCTCTTC	2 cut(s)	546, 1062
BsrDI	GCAATG	4 cut(s)	816, 862, 1212, 1418
BsrFI	RCCGGY	1 cut(s)	869
BsrI	ACTGG	1 cut(s)	531
BssAI	RCCGGY	1 cut(s)	869
BssECI	CCNNGG	4 cut(s)	258, 546, 612, 1287
BssMI	GATC	5 cut(s)	388, 521, 674, 1103, 1168
BssNAI	GTATAC	1 cut(s)	270
Bst1107I	GTATAC	1 cut(s)	270
Bst2UI	CCWGG	4 cut(s)	259, 614, 1005, 1288
Bst4CI	ACNGT	4 cut(s)	169, 637, 973, 982
Bst6I	CTCTTC	2 cut(s)	546, 1062
BstAPI	GCANNNNNTGC	2 cut(s)	71, 677
BstBAI	YACGTR	1 cut(s)	946
BstC8I	GCNNGC	2 cut(s)	682, 1176
BstDEI	CTNAG	4 cut(s)	153, 181, 273, 661
BstF5I	GGATG	4 cut(s)	349, 1000, 1020, 1358
BstFNI	CGCG	1 cut(s)	562
BstHHI	GCGC	1 cut(s)	564
BstKTI	GATC	5 cut(s)	391, 524, 677, 1106, 1171
BstMAI	GTCTC	3 cut(s)	9, 162, 182
BstMBI	GATC	5 cut(s)	388, 521, 674, 1103, 1168
BstNI	CCWGG	4 cut(s)	259, 614, 1005, 1288
BstSCI	CCNGG	4 cut(s)	257, 612, 1003, 1286
BstSFI	CTRYAG	5 cut(s)	396, 474, 669, 915, 1116
BstUI	CGCG	1 cut(s)	562
BstV1I	GCAGC	4 cut(s)	655, 833, 901, 923
BstV2I	GAAGAC	2 cut(s)	93, 635
BstX2I	RGATCY	3 cut(s)	388, 674, 1168
BstYI	RGATCY	3 cut(s)	388, 674, 1168
BstZ17I	GTATAC	1 cut(s)	270
Bsu15I	ATCGAT	1 cut(s)	1102
BsuI	GTATCC	2 cut(s)	229, 664
BsuRI	GGCC	4 cut(s)	611, 771, 864, 1286
BsuTUI	ATCGAT	1 cut(s)	1102
BtsCI	GGATG	4 cut(s)	349, 1000, 1020, 1358
BtsI	GCAGTG	1 cut(s)	60
BtsIMutI	CAGTG	3 cut(s)	60, 165, 524
BveI	ACCTGC	1 cut(s)	250
Cac8I	GCNNGC	2 cut(s)	682, 1176
CaiI	CAGNNNCTG	1 cut(s)	677
CciI	TCATGA	1 cut(s)	1248
CfoI	GCGC	1 cut(s)	564
Cfr10I	RCCGGY	1 cut(s)	869
Cfr13I	GGNCC	2 cut(s)	610, 770
ClaI	ATCGAT	1 cut(s)	1102
CseI	GACGC	1 cut(s)	98
Csp6I	GTAC	3 cut(s)	491, 978, 1240
CviAII	CATG	5 cut(s)	695, 866, 1249, 1282, 1320
CviQI	GTAC	3 cut(s)	491, 978, 1240
DdeI	CTNAG	4 cut(s)	153, 181, 273, 661
DpnI	GATC	5 cut(s)	390, 523, 676, 1105, 1170
DpnII	GATC	5 cut(s)	388, 521, 674, 1103, 1168
DrdI	GACNNNNNNGTC	1 cut(s)	324
DseDI	GACNNNNNNGTC	1 cut(s)	324
EaeI	YGGCCR	1 cut(s)	1284
Eam1104I	CTCTTC	2 cut(s)	546, 1062
EarI	CTCTTC	2 cut(s)	546, 1062
EciI	GGCGGA	1 cut(s)	1161
Eco57I	CTGAAG	2 cut(s)	647, 1368
EcoO109I	RGGNCCY	2 cut(s)	610, 770
EcoRII	CCWGG	4 cut(s)	257, 612, 1003, 1286
EcoT22I	ATGCAT	1 cut(s)	1360
FaeI	CATG	5 cut(s)	698, 869, 1252, 1285, 1323
FaqI	GGGAC	1 cut(s)	330
FatI	CATG	5 cut(s)	694, 865, 1248, 1281, 1319
FblI	GTMKAC	1 cut(s)	269
Fnu4HI	GCNGC	5 cut(s)	589, 669, 822, 912, 915
FokI	GGATG	4 cut(s)	356, 987, 1007, 1345
Fsp4HI	GCNGC	5 cut(s)	589, 669, 822, 912, 915
FspBI	CTAG	6 cut(s)	18, 467, 581, 908, 929, 1406
GlaI	GCGC	1 cut(s)	563
GluI	GCNGC	5 cut(s)	589, 669, 822, 912, 915
GsaI	CCCAGC	1 cut(s)	830
GsuI	CTGGAG	2 cut(s)	1013, 1026
HaeIII	GGCC	4 cut(s)	611, 771, 864, 1286
HapII	CCGG	1 cut(s)	870
HgaI	GACGC	1 cut(s)	98
HhaI	GCGC	1 cut(s)	564
Hin1II	CATG	5 cut(s)	698, 869, 1252, 1285, 1323
Hin6I	GCGC	1 cut(s)	562
HinP1I	GCGC	1 cut(s)	562
HindIII	AAGCTT	1 cut(s)	1212
HinfI	GANTC	5 cut(s)	137, 190, 570, 953, 1271
HpaII	CCGG	1 cut(s)	870
HphI	GGTGA	1 cut(s)	535
Hpy166II	GTNNAC	3 cut(s)	270, 543, 1127
Hpy188I	TCNGA	7 cut(s)	9, 235, 550, 627, 1147, 1186, 1299
Hpy188III	TCNNGA	9 cut(s)	77, 134, 386, 438, 992, 1040, 1166, 1249, 1367
Hpy8I	GTNNAC	3 cut(s)	270, 543, 1127
HpyAV	CCTTC	5 cut(s)	67, 368, 667, 782, 845
HpyCH4III	ACNGT	4 cut(s)	169, 637, 973, 982
HpyCH4IV	ACGT	3 cut(s)	945, 1129, 1238
HpyF3I	CTNAG	4 cut(s)	153, 181, 273, 661
HpySE526I	ACGT	3 cut(s)	945, 1129, 1238
Hsp92II	CATG	5 cut(s)	698, 869, 1252, 1285, 1323
HspAI	GCGC	1 cut(s)	562
Kzo9I	GATC	5 cut(s)	388, 521, 674, 1103, 1168
LguI	GCTCTTC	2 cut(s)	546, 1062
LmnI	GCTCC	4 cut(s)	42, 59, 994, 1007
Lsp1109I	GCAGC	4 cut(s)	655, 833, 901, 923
LweI	GCATC	8 cut(s)	334, 412, 655, 691, 889, 1029, 1045, 1367
MaeI	CTAG	6 cut(s)	18, 467, 581, 908, 929, 1406
MaeII	ACGT	3 cut(s)	945, 1129, 1238
MaeIII	GTNAC	5 cut(s)	253, 353, 808, 1322, 1331
MalI	GATC	5 cut(s)	390, 523, 676, 1105, 1170
MboI	GATC	5 cut(s)	388, 521, 674, 1103, 1168
MboII	GAAGA	8 cut(s)	23, 98, 563, 640, 710, 1079, 1086, 1361
MfeI	CAATTG	2 cut(s)	798, 1110
MflI	RGATCY	3 cut(s)	388, 674, 1168
MlsI	TGGCCA	1 cut(s)	1286
MluCI	AATT	9 cut(s)	27, 752, 798, 920, 1044, 1086, 1095, 1110, 1197
MluNI	TGGCCA	1 cut(s)	1286
MlyI	GAGTC	1 cut(s)	184
Mox20I	TGGCCA	1 cut(s)	1286
Mph1103I	ATGCAT	1 cut(s)	1360
MscI	TGGCCA	1 cut(s)	1286
MseI	TTAA	4 cut(s)	593, 722, 1014, 1235
MslI	CAYNNNNRTG	5 cut(s)	81, 420, 699, 870, 1423
Msp20I	TGGCCA	1 cut(s)	1286
MspA1I	CMGCKG	2 cut(s)	576, 914
MspI	CCGG	1 cut(s)	870
MspR9I	CCNGG	4 cut(s)	259, 614, 1005, 1288
MunI	CAATTG	2 cut(s)	798, 1110
MvaI	CCWGG	4 cut(s)	259, 614, 1005, 1288
MvnI	CGCG	1 cut(s)	562
NdeII	GATC	5 cut(s)	388, 521, 674, 1103, 1168
NlaIII	CATG	5 cut(s)	698, 869, 1252, 1285, 1323
NlaIV	GGNNCC	3 cut(s)	772, 1009, 1170
NmeAIII	GCCGAG	1 cut(s)	1283
NsiI	ATGCAT	1 cut(s)	1360
PagI	TCATGA	1 cut(s)	1248
PaqCI	CACCTGC	1 cut(s)	250
PciSI	GCTCTTC	2 cut(s)	546, 1062
PfeI	GAWTC	4 cut(s)	137, 570, 953, 1271
PflMI	CCANNNNNTGG	1 cut(s)	437
PfoI	TCCNGGA	1 cut(s)	1003
PkrI	GCNGC	5 cut(s)	590, 670, 823, 913, 916
PleI	GAGTC	1 cut(s)	184
PpsI	GAGTC	1 cut(s)	184
Ppu21I	YACGTR	1 cut(s)	946
Psp6I	CCWGG	4 cut(s)	257, 612, 1003, 1286
PspFI	CCCAGC	1 cut(s)	826
PspGI	CCWGG	4 cut(s)	257, 612, 1003, 1286
PspN4I	GGNNCC	3 cut(s)	772, 1009, 1170
PspPI	GGNCC	2 cut(s)	610, 770
PstI	CTGCAG	3 cut(s)	400, 673, 919
PstNI	CAGNNNCTG	1 cut(s)	677
PsuI	RGATCY	3 cut(s)	388, 674, 1168
PvuII	CAGCTG	2 cut(s)	576, 914
RsaI	GTAC	3 cut(s)	492, 979, 1241
RsaNI	GTAC	3 cut(s)	491, 978, 1240
RseI	CAYNNNNRTG	5 cut(s)	81, 420, 699, 870, 1423
SapI	GCTCTTC	2 cut(s)	546, 1062
SaqAI	TTAA	4 cut(s)	593, 722, 1014, 1235
SatI	GCNGC	5 cut(s)	589, 669, 822, 912, 915
Sau3AI	GATC	5 cut(s)	388, 521, 674, 1103, 1168
Sau96I	GGNCC	2 cut(s)	610, 770
SchI	GAGTC	1 cut(s)	184
ScrFI	CCNGG	4 cut(s)	259, 614, 1005, 1288
SfaNI	GCATC	8 cut(s)	334, 412, 655, 691, 889, 1029, 1045, 1367
SfcI	CTRYAG	5 cut(s)	396, 474, 669, 915, 1116
SmiMI	CAYNNNNRTG	5 cut(s)	81, 420, 699, 870, 1423
Sse9I	AATT	9 cut(s)	27, 752, 798, 920, 1044, 1086, 1095, 1110, 1197
SsiI	CCGC	2 cut(s)	589, 1172
SspMI	CTAG	6 cut(s)	18, 467, 581, 908, 929, 1406
StyD4I	CCNGG	4 cut(s)	257, 612, 1003, 1286
TaaI	ACNGT	4 cut(s)	169, 637, 973, 982
TaiI	ACGT	3 cut(s)	948, 1132, 1241
TaqI	TCGA	3 cut(s)	224, 1102, 1220
TasI	AATT	9 cut(s)	27, 752, 798, 920, 1044, 1086, 1095, 1110, 1197
TatI	WGTACW	1 cut(s)	490
TauI	GCSGC	1 cut(s)	591
TfiI	GAWTC	4 cut(s)	137, 570, 953, 1271
Tru1I	TTAA	4 cut(s)	593, 722, 1014, 1235
Tru9I	TTAA	4 cut(s)	593, 722, 1014, 1235
TscAI	CASTG	3 cut(s)	67, 172, 531
TseI	GCWGC	4 cut(s)	668, 821, 911, 914
TspRI	CASTG	3 cut(s)	67, 172, 531
Van91I	CCANNNNNTGG	1 cut(s)	437
XapI	RAATTY	1 cut(s)	1095
XcmI	CCANNNNNNNNNTGG	1 cut(s)	523
XmiI	GTMKAC	1 cut(s)	269
XspI	CTAG	6 cut(s)	18, 467, 581, 908, 929, 1406
Zsp2I	ATGCAT	1 cut(s)	1360

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.