Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0006522

Physical Location & Seq

Genomic Coordinates Reverse (-)

20718527 .. 20723714

Gene Structure 5188 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1216800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1494 bp

ATGAGAGGGGCTTTTGTAGAGCTCTACAGAGGTCTTGGTTTGCTTAAAACTTACAGCTCACTAAATATGGTAGCTTTTACAAAGATCCTAAAGAAATTCGACAAGGTATCAAACCAGCAGGCATCAGAAAGTTATCTGAAAGCAGTGAAGATATCACATTTCATTAGTTCTGATAAGGTTGTTAGACTAATGGACGAAGTGGAGTCCATATTCACAAAGCACTTCGCCAGTAATGACAAGAAAAAGGCAATGAAGTTTTTGAGGCCACAACAAAACAAAGATTCTCATACGGTAACCTTTTTTGTTGGTTTGTTTACAGGTTGTTTTGTATCATTGTTTAGTGTTTATGCAATTTTAGCGCACTTGTCTGGTATATTCTCTCCTAGCACAGAAAGAGATTACATGGAAACTGTCTACCCTGTTTTCAGTGTTTTTGCCTTACTCAGCCTGCACTTGTTTATGTATGGGTGCAACCTATTCATGTGGAAGAGTACAAGAATCAATTACAACTTTATATTTGAATTCCAACCAAGCACGGCCCTAAAGTATCGCGACGCCTTCCTCGTATGCACAACCTTCATGACAACTGTGGTTGCTGCCATGGTTGTGCACCTTATATTAAGGGCAAAAAACTTCTTACCTAGCCAAGTTGATGCCATTCCTGGAGTTTTCCTTCTGTTTGCTGTTGCACTGCTTGTATGCCCGTTAGACATCTTATATCGACCAACTCGTTACTGCTTCATCAGAGTAATTCGTAACATAGTCTTCTCACCACTTTATAAGGTTTTGATGGTAGATTTTTTTATGGCTGATCAACTTACTAGCCAGATCCCATTGCTGAGACACATGGAATCCACAGCTTGTTACTTTCTTGCTGGAAATTTCAGATCACACCAATACGAAACCTGCCGAAGTGGAAGGCTATTTAGAGAGCTGCTTTATGTTATCTCGTTCTTGCCATACTACTGGCGTGCCATGCAATGTGCAAGACGATGGTTTGATGAATGTGACGTTAACCATTTGGCTAACATGGGAAAATATGTTTCTGCCATGGTGGCAGCCGGATCAAGGATTACATATGCTAACAACCCGCACAGCCACCTATGGTTTTATATAGTAGTGATCACCTCTGTGGTGGCTACAGTCTACCAGTTATACTGGGACTTTGTCATGGACTGGGGGCTTTTCGACCCCAAATCCAAGAACTTATGGCTAAGAGACGAACTTATTCTGAAGAACAAAAGCATCTACTATGCTTCCATTGCCTTGAATGCGGTCCTAAGAGTTGCTTGGGTAGAAACAGTAATGGGATTTCATAGAATTCCGGATGTTGAGTCACGATTGCTGGACTTTCTCTTAGCTTCATTAGAGGTTATCCGACGAGGGCATTGGAACTTTTACAGGTTAGAGAATGAGCATCTAAATAATGTTGGCAAGTACAGGGCAGTGAAGACAGTTCCTCTACCATTCCATGAGACAGATTCTGATGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000822 GO:0003674 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006082 GO:0006793 GO:0006797 GO:0006799 GO:0006810 GO:0006811 GO:0006817 GO:0006820 GO:0006950 GO:0007154 GO:0007165 GO:0008144 GO:0008150 GO:0008152 GO:0008509 GO:0009058 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0012505 GO:0015075 GO:0015103 GO:0015114 GO:0015318 GO:0015698 GO:0016020 GO:0016036 GO:0019932 GO:0022857 GO:0023052 GO:0031667 GO:0031668 GO:0031669 GO:0031984 GO:0033554 GO:0034220 GO:0035435 GO:0035556 GO:0036094 GO:0042594 GO:0043167 GO:0043168 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044281 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048016 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0065007 GO:0071496 GO:0071704 GO:0071944 GO:0098656 GO:0098660 GO:0098661 GO:0098791 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EXS SPX

Protein Analysis

498

Amino Acids

57.78

Weight (kDa)

9.27

Isoelectric Point (pI)

32.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPX	PF03105	2 - 49	7.4e-20	SPX domain
EXS	PF03124	139 - 474	2.5e-97	EXS family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006522)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23430
fragaria_vesca	FvH4_4g04040
malus_domestica	MD13G1216800.v1.1 MD16G1222100.v1.1
prunus_persica	Prupe.1G042500_v2.0.a1 Prupe.1G042500_v2.0.a1
pyrus_communis	pycom13g19040 pycom16g18640
rosa_chinensis	RchiOBHm_Chr4g0394311 RchiOBHm_Chr4g0394321
rosa_laevigata	RLG00000009703 RLG00000009704
rosa_multiflora	Rmu_ssc0000423.1_g000005 Rmu_ssc0000423.1_g000013
rosa_roxburghii	Rroxscaffold_5G00339380 Rroxscaffold_6G00430740
rosa_rugosa	Rorug03G0367000.1
rosa_samantha	Rh4AG051600 Rh4AG051900 Rh4BG048800 Rh4BG049300 Rh4CG055500 Rh4CG055900 Rh4DG049100
rosa_wichuraiana	Rw4G004130 Rw7G008900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	780
Acc36I	ACCTGC	1 cut(s)	914
AccI	GTMKAC	2 cut(s)	414, 1146
AccII	CGCG	1 cut(s)	552
AccIII	TCCGGA	1 cut(s)	1324
AciI	CCGC	2 cut(s)	1091, 1274
AclWI	GGATC	3 cut(s)	79, 823, 1072
AcsI	RAATTY	4 cut(s)	95, 521, 880, 1320
AcuI	CTGAAG	1 cut(s)	1253
AcyI	GRCGYC	1 cut(s)	555
AfaI	GTAC	2 cut(s)	493, 1439
AfiI	CCNNNNNNNGG	1 cut(s)	1068
AgsI	TTSAA	2 cut(s)	521, 1270
AjnI	CCWGG	1 cut(s)	661
AleI	CACNNNNGTG	1 cut(s)	1130
AluBI	AGCT	6 cut(s)	22, 57, 74, 860, 934, 1361
AluI	AGCT	6 cut(s)	22, 57, 74, 860, 934, 1361
Alw21I	GWGCWC	2 cut(s)	24, 612
Alw26I	GTCTC	3 cut(s)	835, 1212, 1469
Alw44I	GTGCAC	1 cut(s)	608
AlwI	GGATC	3 cut(s)	79, 823, 1072
AlwNI	CAGNNNCTG	1 cut(s)	1484
Aor13HI	TCCGGA	1 cut(s)	1324
AoxI	GGCC	2 cut(s)	263, 537
ApaLI	GTGCAC	1 cut(s)	608
ApeKI	GCWGC	3 cut(s)	596, 934, 1058
ApoI	RAATTY	4 cut(s)	95, 521, 880, 1320
Asp700I	GAANNNNTTC	1 cut(s)	1227
AspLEI	GCGC	1 cut(s)	361
AspS9I	GGNCC	2 cut(s)	538, 1276
AsuHPI	GGTGA	2 cut(s)	762, 1117
AvaII	GGWCC	1 cut(s)	1276
BaeGI	GKGCMC	1 cut(s)	612
BanII	GRGCYC	1 cut(s)	24
BbsI	GAAGAC	2 cut(s)	757, 1457
Bbv12I	GWGCWC	2 cut(s)	24, 612
BbvI	GCAGC	3 cut(s)	583, 921, 1070
BccI	CCATC	3 cut(s)	784, 987, 1481
BceAI	ACGGC	1 cut(s)	552
BciT130I	CCWGG	1 cut(s)	663
BclI	TGATCA	2 cut(s)	811, 1122
BcoDI	GTCTC	3 cut(s)	835, 1212, 1469
BfaI	CTAG	3 cut(s)	384, 642, 822
BfmI	CTRYAG	2 cut(s)	25, 1140
BfuAI	ACCTGC	1 cut(s)	914
BglI	GCCNNNNNGGC	1 cut(s)	1055
BisI	GCNGC	3 cut(s)	597, 935, 1059
BlsI	GCNGC	3 cut(s)	598, 936, 1060
Bme1390I	CCNGG	1 cut(s)	663
Bme18I	GGWCC	1 cut(s)	1276
BmgT120I	GGNCC	2 cut(s)	538, 1276
BmrFI	CCNGG	1 cut(s)	663
BmrI	ACTGGG	2 cut(s)	1168, 1186
BmsI	GCATC	4 cut(s)	131, 643, 1254, 1426
BmuI	ACTGGG	2 cut(s)	1168, 1186
BpiI	GAAGAC	2 cut(s)	757, 1457
BpmI	CTGGAG	1 cut(s)	684
BsaHI	GRCGYC	1 cut(s)	555
BsaJI	CCNNGG	2 cut(s)	600, 1050
BsaWI	WCCGGW	1 cut(s)	1324
Bsc4I	CCNNNNNNNGG	1 cut(s)	1068
Bse1I	ACTGG	5 cut(s)	228, 971, 1150, 1163, 1181
Bse3DI	GCAATG	4 cut(s)	255, 833, 986, 1260
BseAI	TCCGGA	1 cut(s)	1324
BseBI	CCWGG	1 cut(s)	663
BseDI	CCNNGG	2 cut(s)	600, 1050
BseGI	GGATG	1 cut(s)	1333
BseLI	CCNNNNNNNGG	1 cut(s)	1068
BseMI	GCAATG	4 cut(s)	255, 833, 986, 1260
BseMII	CTCAG	2 cut(s)	457, 830
BseNI	ACTGG	5 cut(s)	228, 971, 1150, 1163, 1181
BseSI	GKGCMC	1 cut(s)	612
BseXI	GCAGC	3 cut(s)	583, 921, 1070
BsgI	GTGCAG	1 cut(s)	434
Bsh1236I	CGCG	1 cut(s)	552
BshFI	GGCC	2 cut(s)	265, 539
BsiHKAI	GWGCWC	2 cut(s)	24, 612
BsiSI	CCGG	2 cut(s)	1062, 1325
BslFI	GGGAC	1 cut(s)	1175
BslI	CCNNNNNNNGG	1 cut(s)	1068
BsmAI	GTCTC	3 cut(s)	835, 1212, 1469
BsmBI	CGTCTC	1 cut(s)	1212
BsmFI	GGGAC	1 cut(s)	1175
BsmI	GAATGC	1 cut(s)	1276
BsnI	GGCC	2 cut(s)	265, 539
Bsp1286I	GDGCHC	2 cut(s)	24, 612
Bsp13I	TCCGGA	1 cut(s)	1324
Bsp143I	GATC	6 cut(s)	84, 811, 828, 887, 1064, 1122
Bsp19I	CCATGG	2 cut(s)	600, 1050
Bsp68I	TCGCGA	1 cut(s)	552
BspACI	CCGC	2 cut(s)	1091, 1274
BspANI	GGCC	2 cut(s)	265, 539
BspCNI	CTCAG	2 cut(s)	456, 831
BspEI	TCCGGA	1 cut(s)	1324
BspFNI	CGCG	1 cut(s)	552
BspHI	TCATGA	1 cut(s)	579
BspMI	ACCTGC	1 cut(s)	914
BspPI	GGATC	3 cut(s)	79, 823, 1072
BsrDI	GCAATG	4 cut(s)	255, 833, 986, 1260
BsrI	ACTGG	5 cut(s)	228, 971, 1150, 1163, 1181
BssECI	CCNNGG	2 cut(s)	600, 1050
BssMI	GATC	6 cut(s)	84, 811, 828, 887, 1064, 1122
BssNI	GRCGYC	1 cut(s)	555
BssT1I	CCWWGG	2 cut(s)	600, 1050
Bst2UI	CCWGG	1 cut(s)	663
Bst4CI	ACNGT	6 cut(s)	292, 412, 589, 1144, 1303, 1456
Bst6I	CTCTTC	1 cut(s)	482
BstACI	GRCGYC	1 cut(s)	555
BstC8I	GCNNGC	3 cut(s)	120, 449, 972
BstDEI	CTNAG	5 cut(s)	443, 839, 1214, 1280, 1357
BstDSI	CCRYGG	2 cut(s)	600, 1050
BstEII	GGTNACC	1 cut(s)	292
BstF5I	GGATG	1 cut(s)	1333
BstFNI	CGCG	1 cut(s)	552
BstHHI	GCGC	1 cut(s)	361
BstKTI	GATC	6 cut(s)	87, 814, 831, 890, 1067, 1125
BstMAI	GTCTC	3 cut(s)	835, 1212, 1469
BstMBI	GATC	6 cut(s)	84, 811, 828, 887, 1064, 1122
BstMWI	GCNNNNNNNGC	5 cut(s)	356, 976, 1055, 1262, 1271
BstNI	CCWGG	1 cut(s)	663
BstPI	GGTNACC	1 cut(s)	292
BstSCI	CCNGG	1 cut(s)	661
BstSFI	CTRYAG	2 cut(s)	25, 1140
BstSLI	GKGCMC	1 cut(s)	612
BstUI	CGCG	1 cut(s)	552
BstV1I	GCAGC	3 cut(s)	583, 921, 1070
BstV2I	GAAGAC	2 cut(s)	757, 1457
BstX2I	RGATCY	2 cut(s)	84, 828
BstXI	CCANNNNNNTGG	1 cut(s)	966
BstYI	RGATCY	2 cut(s)	84, 828
BsuRI	GGCC	2 cut(s)	265, 539
BtgI	CCRYGG	2 cut(s)	600, 1050
BtsCI	GGATG	1 cut(s)	1333
BtsI	GCAGTG	3 cut(s)	150, 689, 1452
BtsIMutI	CAGTG	4 cut(s)	150, 433, 689, 1452
BtuMI	TCGCGA	1 cut(s)	552
BveI	ACCTGC	1 cut(s)	914
Cac8I	GCNNGC	3 cut(s)	120, 449, 972
CaiI	CAGNNNCTG	1 cut(s)	1484
CciI	TCATGA	1 cut(s)	579
CfoI	GCGC	1 cut(s)	361
Cfr13I	GGNCC	2 cut(s)	538, 1276
CseI	GACGC	1 cut(s)	563
Csp6I	GTAC	2 cut(s)	492, 1438
CviQI	GTAC	2 cut(s)	492, 1438
DdeI	CTNAG	5 cut(s)	443, 839, 1214, 1280, 1357
DpnI	GATC	6 cut(s)	86, 813, 830, 889, 1066, 1124
DpnII	GATC	6 cut(s)	84, 811, 828, 887, 1064, 1122
Eam1104I	CTCTTC	1 cut(s)	482
EarI	CTCTTC	1 cut(s)	482
Ecl136II	GAGCTC	1 cut(s)	22
Eco130I	CCWWGG	2 cut(s)	600, 1050
Eco24I	GRGCYC	1 cut(s)	24
Eco32I	GATATC	1 cut(s)	153
Eco47I	GGWCC	1 cut(s)	1276
Eco53kI	GAGCTC	1 cut(s)	22
Eco57I	CTGAAG	1 cut(s)	1253
Eco91I	GGTNACC	1 cut(s)	292
EcoICRI	GAGCTC	1 cut(s)	22
EcoO65I	GGTNACC	1 cut(s)	292
EcoRI	GAATTC	2 cut(s)	521, 1320
EcoRII	CCWGG	1 cut(s)	661
EcoRV	GATATC	1 cut(s)	153
EcoT14I	CCWWGG	2 cut(s)	600, 1050
EcoT38I	GRGCYC	1 cut(s)	24
ErhI	CCWWGG	2 cut(s)	600, 1050
Esp3I	CGTCTC	1 cut(s)	1212
FalI	AAGNNNNNCTT	2 cut(s)	1273, 1305
FaqI	GGGAC	1 cut(s)	1175
FauI	CCCGC	1 cut(s)	1098
FauNDI	CATATG	1 cut(s)	1078
FbaI	TGATCA	2 cut(s)	811, 1122
FblI	GTMKAC	2 cut(s)	414, 1146
Fnu4HI	GCNGC	3 cut(s)	597, 935, 1059
FokI	GGATG	1 cut(s)	1340
FriOI	GRGCYC	1 cut(s)	24
Fsp4HI	GCNGC	3 cut(s)	597, 935, 1059
FspBI	CTAG	3 cut(s)	384, 642, 822
GlaI	GCGC	1 cut(s)	360
GluI	GCNGC	3 cut(s)	597, 935, 1059
GsuI	CTGGAG	1 cut(s)	684
HaeIII	GGCC	2 cut(s)	265, 539
HapII	CCGG	2 cut(s)	1062, 1325
HgaI	GACGC	1 cut(s)	563
HhaI	GCGC	1 cut(s)	361
Hin1I	GRCGYC	1 cut(s)	555
Hin6I	GCGC	1 cut(s)	359
HinP1I	GCGC	1 cut(s)	359
HincII	GTYRAC	1 cut(s)	1015
HindII	GTYRAC	1 cut(s)	1015
HinfI	GANTC	6 cut(s)	203, 281, 498, 851, 1334, 1481
HpaI	GTTAAC	1 cut(s)	1015
HpaII	CCGG	2 cut(s)	1062, 1325
HphI	GGTGA	2 cut(s)	762, 1117
Hpy166II	GTNNAC	5 cut(s)	315, 415, 610, 1015, 1147
Hpy188I	TCNGA	8 cut(s)	127, 138, 172, 746, 887, 1233, 1379, 1486
Hpy188III	TCNNGA	4 cut(s)	551, 580, 1325, 1338
Hpy8I	GTNNAC	5 cut(s)	315, 415, 610, 1015, 1147
Hpy99I	CGWCG	2 cut(s)	557, 1383
HpyAV	CCTTC	4 cut(s)	568, 586, 683, 912
HpyCH4III	ACNGT	6 cut(s)	292, 412, 589, 1144, 1303, 1456
HpyCH4IV	ACGT	1 cut(s)	1011
HpyCH4V	TGCA	8 cut(s)	350, 451, 471, 570, 610, 689, 979, 986
HpyF10VI	GCNNNNNNNGC	5 cut(s)	356, 976, 1055, 1262, 1271
HpyF3I	CTNAG	5 cut(s)	443, 839, 1214, 1280, 1357
HpySE526I	ACGT	1 cut(s)	1011
Hsp92I	GRCGYC	1 cut(s)	555
HspAI	GCGC	1 cut(s)	359
Kpn2I	TCCGGA	1 cut(s)	1324
Ksp22I	TGATCA	2 cut(s)	811, 1122
KspAI	GTTAAC	1 cut(s)	1015
Kzo9I	GATC	6 cut(s)	84, 811, 828, 887, 1064, 1122
Lsp1109I	GCAGC	3 cut(s)	583, 921, 1070
LweI	GCATC	4 cut(s)	131, 643, 1254, 1426
MaeI	CTAG	3 cut(s)	384, 642, 822
MaeII	ACGT	1 cut(s)	1011
MaeIII	GTNAC	6 cut(s)	292, 731, 755, 863, 1007, 1335
MalI	GATC	6 cut(s)	86, 813, 830, 889, 1066, 1124
MboI	GATC	6 cut(s)	84, 811, 828, 887, 1064, 1122
MboII	GAAGA	5 cut(s)	160, 499, 757, 1246, 1462
MflI	RGATCY	2 cut(s)	84, 828
MhlI	GDGCHC	2 cut(s)	24, 612
MluCI	AATT	7 cut(s)	95, 351, 502, 521, 750, 880, 1320
MlyI	GAGTC	2 cut(s)	212, 1343
MmeI	TCCRAC	2 cut(s)	550, 1402
MnlI	CCTC	7 cut(s)	23, 255, 572, 1138, 1363, 1376, 1470
MroI	TCCGGA	1 cut(s)	1324
MroXI	GAANNNNTTC	1 cut(s)	1227
MseI	TTAA	3 cut(s)	45, 620, 1014
MslI	CAYNNNNRTG	2 cut(s)	605, 1130
MspI	CCGG	2 cut(s)	1062, 1325
MspR9I	CCNGG	1 cut(s)	663
Mva1269I	GAATGC	1 cut(s)	1276
MvaI	CCWGG	1 cut(s)	663
MvnI	CGCG	1 cut(s)	552
MwoI	GCNNNNNNNGC	5 cut(s)	356, 976, 1055, 1262, 1271
NcoI	CCATGG	2 cut(s)	600, 1050
NdeI	CATATG	1 cut(s)	1078
NdeII	GATC	6 cut(s)	84, 811, 828, 887, 1064, 1122
NmuCI	GTSAC	2 cut(s)	1007, 1335
NruI	TCGCGA	1 cut(s)	552
OliI	CACNNNNGTG	1 cut(s)	1130
PagI	TCATGA	1 cut(s)	579
PcsI	WCGNNNNNNNCGW	2 cut(s)	561, 727
PctI	GAATGC	1 cut(s)	1276
PdmI	GAANNNNTTC	1 cut(s)	1227
PfeI	GAWTC	4 cut(s)	281, 498, 851, 1481
PflFI	GACNNNGTC	1 cut(s)	1166
PfoI	TCCNGGA	1 cut(s)	661
PkrI	GCNGC	3 cut(s)	598, 936, 1060
PleI	GAGTC	2 cut(s)	211, 1342
PpsI	GAGTC	2 cut(s)	211, 1342
PsiI	TTATAA	1 cut(s)	780
Psp124BI	GAGCTC	1 cut(s)	24
Psp6I	CCWGG	1 cut(s)	661
PspEI	GGTNACC	1 cut(s)	292
PspGI	CCWGG	1 cut(s)	661
PspPI	GGNCC	2 cut(s)	538, 1276
PstNI	CAGNNNCTG	1 cut(s)	1484
PsuI	RGATCY	2 cut(s)	84, 828
PsyI	GACNNNGTC	1 cut(s)	1166
RruI	TCGCGA	1 cut(s)	552
RsaI	GTAC	2 cut(s)	493, 1439
RsaNI	GTAC	2 cut(s)	492, 1438
RseI	CAYNNNNRTG	2 cut(s)	605, 1130
SacI	GAGCTC	1 cut(s)	24
SaqAI	TTAA	3 cut(s)	45, 620, 1014
SatI	GCNGC	3 cut(s)	597, 935, 1059
Sau3AI	GATC	6 cut(s)	84, 811, 828, 887, 1064, 1122
Sau96I	GGNCC	2 cut(s)	538, 1276
SchI	GAGTC	2 cut(s)	212, 1343
ScrFI	CCNGG	1 cut(s)	663
SduI	GDGCHC	2 cut(s)	24, 612
SfaNI	GCATC	4 cut(s)	131, 643, 1254, 1426
SfcI	CTRYAG	2 cut(s)	25, 1140
SinI	GGWCC	1 cut(s)	1276
SmiMI	CAYNNNNRTG	2 cut(s)	605, 1130
Sse9I	AATT	7 cut(s)	95, 351, 502, 521, 750, 880, 1320
SsiI	CCGC	2 cut(s)	1091, 1274
SspMI	CTAG	3 cut(s)	384, 642, 822
SstI	GAGCTC	1 cut(s)	24
StyD4I	CCNGG	1 cut(s)	661
StyI	CCWWGG	2 cut(s)	600, 1050
TaaI	ACNGT	6 cut(s)	292, 412, 589, 1144, 1303, 1456
TaiI	ACGT	1 cut(s)	1014
TaqI	TCGA	3 cut(s)	99, 721, 1188
TasI	AATT	7 cut(s)	95, 351, 502, 521, 750, 880, 1320
TatI	WGTACW	2 cut(s)	491, 1437
TfiI	GAWTC	4 cut(s)	281, 498, 851, 1481
Tru1I	TTAA	3 cut(s)	45, 620, 1014
Tru9I	TTAA	3 cut(s)	45, 620, 1014
TscAI	CASTG	4 cut(s)	150, 433, 696, 1452
TseFI	GTSAC	2 cut(s)	1007, 1335
TseI	GCWGC	3 cut(s)	596, 934, 1058
Tsp45I	GTSAC	2 cut(s)	1007, 1335
TspDTI	ATGAA	8 cut(s)	151, 266, 469, 568, 730, 1017, 1304, 1353
TspRI	CASTG	4 cut(s)	150, 433, 696, 1452
Tth111I	GACNNNGTC	1 cut(s)	1166
VneI	GTGCAC	1 cut(s)	608
VpaK11BI	GGWCC	1 cut(s)	1276
XapI	RAATTY	4 cut(s)	95, 521, 880, 1320
XmiI	GTMKAC	2 cut(s)	414, 1146
XmnI	GAANNNNTTC	1 cut(s)	1227
XspI	CTAG	3 cut(s)	384, 642, 822

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.