MD13G1231300.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012803
Physical Location & Seq
Forward (+)
22956087 .. 22960917
4831 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1231300.v1.1.491

Sequence Viewer

Length: 3576 bp
ATGACTGGTGAGGTTCCTGGTACTTCTGGTCGACAGTTTTGCAGAGATACAACCAATGATGTTCGAAGTGACAGAAATGCTGCGGACGAAAATGTGACTAATATTTGTCTACAGACCGGTGAGGAATTTTCAATGGAGTTTCTGCAGGATCGTATTGCACAAAGAAGATTAGCCCCTGTACTGACTGGCATTGATCTGTGCCAGTCTGAAAGGTTGGGTTCTAATATACACCAAAACCCTAAACTGCTTTACGAGGATCTTGGTAGCGTTATGGGGATGAAGAGAATAGATTCCGATTGCAGTTCAGAATTCTCGGATTTTAGTCCAGTTGCTCCAAAAGGTTTTCTAGCGGACACGGAGAAAAATGTTTATCCTAGTAACATGAGCAGATACCACTGGGAATATGGTGCCATGGGAAAAGCATCGAGTAAGTTTGCTGATGAAGTTAATCGCGATCGGGTTATTCCAAGACCAACTACTCCACCTTTTTATGTTGTGGACTCCCCTCAATCATACACTCCTTATGGTCAAGTATTTGCTGAAGGTTCTTTCTCTTTCAAAAGGAAATTTCTCTGCAGCTTTGGGGGAAGAATATTACCTCGGCCAAATGATGGGAAACTCCGATATGTAGGTGGAGATACACGGATTTTATCTATTCGCGAAGGCACCAATTTTGAGCAACTAATGAAGAAGACATTTGTAATTTGCAACCAACATCACACTATCAAGTACCAGCTTCCCGGTGAGGATCTTGATGCACTTGTATCCGTTTGTTCCGATGAGGACCTTCTTCATATGATAGAGGAATATCATGAGCTAGAGAGAACAGAGGGTTCTCAAAGACTGAGGCTCTTCCTTGTACCTTTAACTGAAACAGAGAGCCCAAGTTCTGTCGAGGCAAGGGTAACACATCCAATCGGTGCTGACTGCGAATTTGTTTTCGCTGTTAATGGTATGTTAGACCCAAGTCCTCAGAAAAGCTCTAGCGAGCAAAGTTTGGCCAGCCAGAATGGAAATCCATCAGATTATAGTCCAACCTTTCATAGAGACTCTCCTACCGGTGCATATTTTGAGACCAAAGACTATAGCCCTAGTTCCTCAAGTGCAGTGGGCACATTAACTAAACCTGCTCCTCAGTTCTTAGCTACTCTTCAGATCCCAAAGAAGTCTTTTAATCAATCCCCTCCCATATCCCCGGTTCCACTTCAACACAGAGATCCCAAGAGTTCCAATGTGCAGCTTTATTTGGACCAATCATATTGTGACGGTAATGCAGGCATTGCTCCTTATGGTATGGAAAAATATCCATGTGACAATACCTATTATCTTGATGCGGGAGGTTATAACAATAATCTTCATCACGGGCCTCCATTGTTAAATCATCATCATCATAACCAATATTTGGCTGAAACTTATAGGGCTAAAAAGTCTCACATTCTGCACTCCCACAATCGTAGTTTTAGCGAAAATTTTGTGCCTTCTGCACGGTATGGCAAAAGTGGTATGGAATCTGAGAGACTTGTGATTCTGGAAAAGGCCCTCCATTCGGAAAAGTCTCTCTCTCGTCCTGAAGATAGAGAGGGACTATCTTCAGGGTCTGGGGAAAGAGATGCTTCTTACCACAGAATAATGCATGCAGTACTTTCTGACTCTCAGTTGCAGGAACATGTTCAGAGGTACACCGAAGGAGAAGTTATCCCAGTGTCCTCACTGAAATACAGACTAGAAAAATCACCTTCATTGAAGATACAAAGGTCCTCGCAAGAACGGTCTGTGCAACAGGAAGTGATTCTTGATGGGAAACATCAAATTGCTAAACATGGTAATCAATTCACGATCAGGAAACCTGACCAATGTAAAGAGGAACGAGGTCTGGAGATGTTGAACCAGACGGACAGGAATGACACTGGTGCTGATAAAAAATGGAAACACTTAGAAGGAAATGTTGAAGTTACATCAAATGATATTGTCATAGAATTTAAGAAGGAACAAAATGCAAACTGCCTTCTCAGTGATTGCTTAGCCATGGAACATTCACAGTTCCCTGAAAGTGGGATTTTTGGTTCATCAAACCCTGAAACTGAAGGTTCTGAAGATACTACGGGCCCCCAAGGCTATGGATTTGACACAACTTCTCAGTTTTCTCTTAGGAACCAAAACTGTACTAGAGATCAGCAGTGTGCTACAGTTGAGACGGTAAGCAGTCAAACAGTTTCTCATCAGTCACGTGAACGACTACCTGTTGCATCTCAGGAGTTCTTAGTCTCAACATCTAAATTGGCTACAACTGCTGCACCAGACAGAGAATTTTCTCTCCATGATAAGGAGTCAAGAAATTATCCTGAGTATTTGGTTGAAAACATATGTCTCAACAAACAGTCTTCTGAAGTAACCAACTGTGAAGGTGCTATCCCTGCCCAATCACAGCCACTGGATAATCGGCATGACAACAAGGTGACGGACTCGATGATTATTGTAGAAGACTTGACTAATAATACACCACCCGGTATCCCTGCCTCCAAAGTGGTCTATAATGTTTCGCATATAGAAGATGAGGCCAGTGATGATTGTTCATCAACTGGAGAAACAGAGACTGGAAGTTCAGCTACAGCATCTGATAACAAGGCTGTGACAGCTGACGGAAGTCATAGGCATGACACTATTAGCGATGTTGCAATAGCTGAAATGGAAGCAGGCATCTATGGTTTGCAGATCATAAAGAATGATGATCTCGAAGAACTGCAAGAGTTAGGATCTGGTACATATGGAACTGTCTATCATGGAAAGTGGAGAGGAACAGATGTTGCAATTAAGAGGATAAAAAAGAGTTGTTTCTCCGGCAGATCATCAGAGCAAGATCGACTGACTAAGGACTTCTGGAGAGAGGCAAAGATTCTGTCAACCCTTCACCATCCCAATGTTGTAGCATTCTATGGCGTGGTGCCTGATGGGCCTGGGGGGACGTTAGCAACTGTAGCTGAATTCATGGTGAATGGATCGTTGAGGCATGTCCTAATAAGAAAAGATAGAGTGCTTGATCGTCAAAAGAGGCTCATAATCTTAATGGACACAGCTTTTGGCATGGAGTATTTGCATTTGAAAAATATTGTTCACTTTGATTTGAAGTGTGACAATTTACTAGTTAATTTGAGGGATCCCGAACGGCCCATATGCAAGGTTGGGGATTTTGGTTTGTCAAGAATTAAGCGCAACACACTAGTATCTGGTGGTGTTCGAGGAACCCTTCCGTGGATGGCACCAGAACTATTAAATGGTAGCAGTAACCGGGTCTCTGAGAAGGTTGATGTCTTCTCATTTGGTATTGTAATGTGGGAGATCCTGACTGGGGAGGAACCGTACGCTAACATGCACTGTGGTGCTATCATAGGGGGAATTGTAAATAACACACTGAGGCCTTCTATTCCAAAGCGCTGTGATTCTGAATGGAAAAAACTAATGGAACAGTGCTGGTCGCCGGATCCTGCTGATCGTCCATCATTCACAGAGATAACACATAGGCTGCGTGACATGTCAATGGCACTCCAGAAAAAGCGGCCCAGTCTTGCAAGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1192

Amino Acids

133.12

Weight (kDa)

5.8

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 203 - 288 1e-20 PB1 domain
Pkinase PF00069 912 - 1172 3.8e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 913 - 1174 2.2e-62 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04700 AT1G04700 AT1G04700
fragaria_vesca FvH4_4g05890
malus_domestica MD13G1231300.v1.1 MD16G1235800.v1.1
prunus_persica Prupe.1G061200_v2.0.a1
pyrus_communis pycom13g20420
rosa_chinensis RchiOBHm_Chr4g0396991
rosa_laevigata RLG00000009461
rosa_multiflora Rmu_sc0002963.1_g000016
rosa_roxburghii Rroxscaffold_5G00341980
rosa_rugosa Rorug03G0346000
rosa_samantha Rh4AG075900 Rh4BG072300 Rh4CG080600 Rh4DG069700
rosa_wichuraiana Rw4G006090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1344
Acc36I ACCTGC 1 cut(s) 1135
AccB1I GGYRCC 4 cut(s) 407, 665, 2941, 3256
AccB7I CCANNNNNTGG 2 cut(s) 611, 1402
AccI GTMKAC 2 cut(s) 31, 109
AccII CGCG 2 cut(s) 453, 660
AciI CCGC 4 cut(s) 83, 350, 1334, 3553
AcoI YGGCCR 2 cut(s) 602, 999
AcsI RAATTY 8 cut(s) 125, 308, 566, 932, 1468, 1976, 2306, 2981
AcuI CTGAAG 7 cut(s) 561, 1136, 1575, 1590, 2103, 2112, 2406
AcvI CACGTG 1 cut(s) 2228
AfaI GTAC 9 cut(s) 22, 180, 731, 861, 1641, 1679, 2164, 2761, 3359
AfeI AGCGCT 1 cut(s) 3431
AfiI CCNNNNNNNGG 7 cut(s) 611, 1402, 1546, 2051, 2323, 3249, 3346
AflIII ACRYGT 2 cut(s) 1666, 3528
AgeI ACCGGT 2 cut(s) 116, 1058
AgsI TTSAA 9 cut(s) 132, 559, 1208, 1744, 1885, 1949, 2357, 3100, 3124
AhlI ACTAGT 2 cut(s) 3139, 3217
AjnI CCWGG 2 cut(s) 16, 2953
AleI CACNNNNGTG 1 cut(s) 3375
AloI GAACNNNNNNTCC 2 cut(s) 2047, 2079
AlwNI CAGNNNCTG 4 cut(s) 1598, 2431, 2594, 2615
Aor51HI AGCGCT 1 cut(s) 3431
ApaI GGGCCC 1 cut(s) 2108
ApeKI GCWGC 5 cut(s) 80, 576, 1237, 2291, 3520
ApoI RAATTY 8 cut(s) 125, 308, 566, 932, 1468, 1976, 2306, 2981
ArsI GACNNNNNNTTYG 6 cut(s) 917, 949, 2882, 2914, 3059, 3091
AsiGI ACCGGT 2 cut(s) 116, 1058
Asp700I GAANNNNTTC 3 cut(s) 289, 1668, 1788
AspLEI GCGC 2 cut(s) 3210, 3432
AsuC2I CCSGG 4 cut(s) 741, 1196, 2505, 3287
AsuHPI GGTGA 7 cut(s) 20, 131, 755, 1725, 2467, 2900, 3001
AsuII TTCGAA 1 cut(s) 64
AvaII GGWCC 3 cut(s) 784, 1249, 1755
BaeGI GKGCMC 2 cut(s) 1115, 2108
BaeI ACNNNNGTAYC 4 cut(s) 1739, 1772, 3204, 3237
BalI TGGCCA 1 cut(s) 1001
BamHI GGATCC 2 cut(s) 3154, 3478
BanI GGYRCC 4 cut(s) 407, 665, 2941, 3256
BanII GRGCYC 2 cut(s) 884, 2108
BarI GAAGNNNNNNTAC 2 cut(s) 2590, 2622
BbrPI CACGTG 1 cut(s) 2228
BbsI GAAGAC 4 cut(s) 698, 2373, 2487, 3301
BbvI GCAGC 5 cut(s) 67, 588, 1249, 2278, 3507
BccI CCATC 7 cut(s) 605, 1027, 1790, 2919, 2942, 3247, 3502
BceAI ACGGC 1 cut(s) 3179
BcgI CGANNNNNNTGC 2 cut(s) 21, 55
BciT130I CCWGG 2 cut(s) 18, 2955
BciVI GTATCC 2 cut(s) 775, 2519
BcnI CCSGG 4 cut(s) 741, 1196, 2505, 3287
BcuI ACTAGT 2 cut(s) 3139, 3217
BfaI CTAG 9 cut(s) 347, 375, 818, 984, 1092, 1724, 2166, 3140, 3218
BfmI CTRYAG 7 cut(s) 110, 143, 574, 1084, 2184, 2607, 2973
BfoI RGCGCY 1 cut(s) 3433
BfuAI ACCTGC 1 cut(s) 1135
BfuI GTATCC 2 cut(s) 775, 2519
BglI GCCNNNNNGGC 2 cut(s) 2112, 2950
BisI GCNGC 6 cut(s) 81, 577, 1238, 2292, 3521, 3554
BlpI GCTNAGC 1 cut(s) 2020
BlsI GCNGC 6 cut(s) 82, 578, 1239, 2293, 3522, 3555
BmcAI AGTACT 1 cut(s) 1641
Bme1390I CCNGG 6 cut(s) 18, 741, 1196, 2505, 2955, 3287
Bme18I GGWCC 3 cut(s) 784, 1249, 1755
BmrFI CCNGG 6 cut(s) 18, 741, 1196, 2505, 2955, 3287
BmrI ACTGGG 4 cut(s) 406, 1694, 3354, 3552
BmsI GCATC 7 cut(s) 431, 745, 1321, 1600, 2255, 2621, 2706
BmuI ACTGGG 4 cut(s) 406, 1694, 3354, 3552
BoxI GACNNNNGTC 2 cut(s) 966, 2643
BpiI GAAGAC 4 cut(s) 698, 2373, 2487, 3301
BpmI CTGGAG 4 cut(s) 1895, 2601, 2899, 3527
Bpu1102I GCTNAGC 1 cut(s) 2020
Bpu14I TTCGAA 1 cut(s) 64
BpuEI CTTGAG 1 cut(s) 1084
BpuMI CCSGG 4 cut(s) 741, 1196, 2505, 3287
BsaAI YACGTR 1 cut(s) 2228
BsaI GGTCTC 2 cut(s) 1067, 3295
BsaJI CCNNGG 7 cut(s) 411, 599, 1194, 2025, 2110, 2954, 3248
BsaWI WCCGGW 2 cut(s) 116, 1058
BsaXI ACNNNNNCTCC 2 cut(s) 3341, 3371
Bsc4I CCNNNNNNNGG 7 cut(s) 611, 1402, 1546, 2051, 2323, 3249, 3346
Bse118I RCCGGY 2 cut(s) 116, 1058
Bse3DI GCAATG 1 cut(s) 1278
BseBI CCWGG 2 cut(s) 18, 2955
BseDI CCNNGG 7 cut(s) 411, 599, 1194, 2025, 2110, 2954, 3248
BseGI GGATG 4 cut(s) 282, 910, 2911, 3258
BseLI CCNNNNNNNGG 7 cut(s) 611, 1402, 1546, 2051, 2323, 3249, 3346
BseMI GCAATG 1 cut(s) 1278
BseRI GAGGAG 1 cut(s) 1122
BseSI GKGCMC 2 cut(s) 1115, 2108
BseXI GCAGC 5 cut(s) 67, 588, 1249, 2278, 3507
BsgI GTGCAG 5 cut(s) 1125, 1256, 1424, 1467, 2277
Bsh1236I CGCG 2 cut(s) 453, 660
Bsh1285I CGRYCG 1 cut(s) 457
BshNI GGYRCC 4 cut(s) 407, 665, 2941, 3256
BshTI ACCGGT 2 cut(s) 116, 1058
BsiEI CGRYCG 1 cut(s) 457
BsiSI CCGG 8 cut(s) 117, 741, 1059, 1196, 2505, 2838, 3286, 3476
BsiWI CGTACG 1 cut(s) 3357
BslFI GGGAC 2 cut(s) 1596, 2974
BslI CCNNNNNNNGG 7 cut(s) 611, 1402, 1546, 2051, 2323, 3249, 3346
BsmBI CGTCTC 1 cut(s) 2186
BsmFI GGGAC 2 cut(s) 1596, 2974
BsmI GAATGC 1 cut(s) 2927
Bso31I GGTCTC 2 cut(s) 1067, 3295
Bsp119I TTCGAA 1 cut(s) 64
Bsp120I GGGCCC 1 cut(s) 2104
Bsp1286I GDGCHC 3 cut(s) 884, 1115, 2108
Bsp1720I GCTNAGC 1 cut(s) 2020
Bsp19I CCATGG 2 cut(s) 411, 2025
Bsp68I TCGCGA 2 cut(s) 453, 660
BspACI CCGC 4 cut(s) 83, 350, 1334, 3553
BspFNI CGCG 2 cut(s) 453, 660
BspHI TCATGA 1 cut(s) 811
BspMAI CTGCAG 2 cut(s) 147, 578
BspMI ACCTGC 1 cut(s) 1135
BspQI GCTCTTC 1 cut(s) 857
BspT104I TTCGAA 1 cut(s) 64
BspT107I GGYRCC 4 cut(s) 407, 665, 2941, 3256
BspTNI GGTCTC 2 cut(s) 1067, 3295
BsrDI GCAATG 1 cut(s) 1278
BsrFI RCCGGY 2 cut(s) 116, 1058
BssAI RCCGGY 2 cut(s) 116, 1058
BssECI CCNNGG 7 cut(s) 411, 599, 1194, 2025, 2110, 2954, 3248
BssT1I CCWWGG 3 cut(s) 411, 2025, 2110
Bst2UI CCWGG 2 cut(s) 18, 2955
Bst6I CTCTTC 3 cut(s) 275, 857, 1155
BstAPI GCANNNNNTGC 1 cut(s) 1280
BstBAI YACGTR 1 cut(s) 2228
BstBI TTCGAA 1 cut(s) 64
BstC8I GCNNGC 5 cut(s) 989, 1003, 1276, 1635, 2695
BstDSI CCRYGG 3 cut(s) 411, 2025, 3248
BstF5I GGATG 4 cut(s) 282, 910, 2911, 3258
BstFNI CGCG 2 cut(s) 453, 660
BstH2I RGCGCY 1 cut(s) 3433
BstHHI GCGC 2 cut(s) 3210, 3432
BstMCI CGRYCG 1 cut(s) 457
BstMWI GCNNNNNNNGC 7 cut(s) 1280, 2112, 2288, 2414, 2633, 2950, 2975
BstNI CCWGG 2 cut(s) 18, 2955
BstNSI RCATGY 5 cut(s) 1637, 1670, 3011, 3370, 3532
BstPAI GACNNNNGTC 2 cut(s) 966, 2643
BstSCI CCNGG 6 cut(s) 16, 739, 1194, 2503, 2953, 3285
BstSFI CTRYAG 7 cut(s) 110, 143, 574, 1084, 2184, 2607, 2973
BstSLI GKGCMC 2 cut(s) 1115, 2108
BstUI CGCG 2 cut(s) 453, 660
BstV1I GCAGC 5 cut(s) 67, 588, 1249, 2278, 3507
BstV2I GAAGAC 4 cut(s) 698, 2373, 2487, 3301
BstX2I RGATCY 8 cut(s) 256, 748, 1155, 1216, 2753, 3154, 3336, 3478
BstXI CCANNNNNNTGG 1 cut(s) 2117
BstYI RGATCY 8 cut(s) 256, 748, 1155, 1216, 2753, 3154, 3336, 3478
BsuI GTATCC 2 cut(s) 775, 2519
BtgI CCRYGG 3 cut(s) 411, 2025, 3248
BtgZI GCGATG 1 cut(s) 2682
BtsCI GGATG 4 cut(s) 282, 910, 2911, 3258
BtsI GCAGTG 2 cut(s) 1113, 2183
BtuMI TCGCGA 2 cut(s) 453, 660
BveI ACCTGC 1 cut(s) 1135
Cac8I GCNNGC 5 cut(s) 989, 1003, 1276, 1635, 2695
CaiI CAGNNNCTG 4 cut(s) 1598, 2431, 2594, 2615
CciI TCATGA 1 cut(s) 811
CfoI GCGC 2 cut(s) 3210, 3432
Cfr10I RCCGGY 2 cut(s) 116, 1058
Csp6I GTAC 9 cut(s) 21, 179, 730, 860, 1640, 1678, 2163, 2760, 3358
CspAI ACCGGT 2 cut(s) 116, 1058
CspCI CAANNNNNGTGG 2 cut(s) 1089, 1124
CviQI GTAC 9 cut(s) 21, 179, 730, 860, 1640, 1678, 2163, 2760, 3358
EaeI YGGCCR 2 cut(s) 602, 999
Eam1104I CTCTTC 3 cut(s) 275, 857, 1155
EarI CTCTTC 3 cut(s) 275, 857, 1155
Eco130I CCWWGG 3 cut(s) 411, 2025, 2110
Eco147I AGGCCT 1 cut(s) 3415
Eco24I GRGCYC 2 cut(s) 884, 2108
Eco31I GGTCTC 2 cut(s) 1067, 3295
Eco47I GGWCC 3 cut(s) 784, 1249, 1755
Eco47III AGCGCT 1 cut(s) 3431
Eco57I CTGAAG 7 cut(s) 561, 1136, 1575, 1590, 2103, 2112, 2406
Eco72I CACGTG 1 cut(s) 2228
EcoO109I RGGNCCY 4 cut(s) 784, 1537, 1755, 2105
EcoRI GAATTC 2 cut(s) 308, 2981
EcoRII CCWGG 2 cut(s) 16, 2953
EcoT14I CCWWGG 3 cut(s) 411, 2025, 2110
EcoT22I ATGCAT 1 cut(s) 1635
EcoT38I GRGCYC 2 cut(s) 884, 2108
ErhI CCWWGG 3 cut(s) 411, 2025, 2110
Esp3I CGTCTC 1 cut(s) 2186
FalI AAGNNNNNCTT 4 cut(s) 1597, 1629, 1776, 1808
FaqI GGGAC 2 cut(s) 1596, 2974
FauI CCCGC 1 cut(s) 1327
FauNDI CATATG 4 cut(s) 795, 2363, 2764, 3170
FblI GTMKAC 2 cut(s) 31, 109
Fnu4HI GCNGC 6 cut(s) 81, 577, 1238, 2292, 3521, 3554
FokI GGATG 4 cut(s) 289, 897, 2898, 3265
FriOI GRGCYC 2 cut(s) 884, 2108
Fsp4HI GCNGC 6 cut(s) 81, 577, 1238, 2292, 3521, 3554
FspBI CTAG 9 cut(s) 347, 375, 818, 984, 1092, 1724, 2166, 3140, 3218
GlaI GCGC 2 cut(s) 3209, 3431
GluI GCNGC 6 cut(s) 81, 577, 1238, 2292, 3521, 3554
GsuI CTGGAG 4 cut(s) 1895, 2601, 2899, 3527
HaeII RGCGCY 1 cut(s) 3433
HapII CCGG 8 cut(s) 117, 741, 1059, 1196, 2505, 2838, 3286, 3476
HhaI GCGC 2 cut(s) 3210, 3432
Hin6I GCGC 2 cut(s) 3208, 3430
HinP1I GCGC 2 cut(s) 3208, 3430
HincII GTYRAC 2 cut(s) 32, 2901
HindII GTYRAC 2 cut(s) 32, 2901
HpaII CCGG 8 cut(s) 117, 741, 1059, 1196, 2505, 2838, 3286, 3476
HphI GGTGA 7 cut(s) 20, 131, 755, 1725, 2467, 2900, 3001
Hpy166II GTNNAC 7 cut(s) 32, 110, 499, 1680, 2231, 2901, 3112
Hpy8I GTNNAC 7 cut(s) 32, 110, 499, 1680, 2231, 2901, 3112
HpyCH4IV ACGT 2 cut(s) 2227, 2963
HpyF10VI GCNNNNNNNGC 7 cut(s) 1280, 2112, 2288, 2414, 2633, 2950, 2975
HpySE526I ACGT 2 cut(s) 2227, 2963
HspAI GCGC 2 cut(s) 3208, 3430
LguI GCTCTTC 1 cut(s) 857
LmnI GCTCC 3 cut(s) 337, 1135, 1288
Lsp1109I GCAGC 5 cut(s) 67, 588, 1249, 2278, 3507
LweI GCATC 7 cut(s) 431, 745, 1321, 1600, 2255, 2621, 2706
MaeI CTAG 9 cut(s) 347, 375, 818, 984, 1092, 1724, 2166, 3140, 3218
MaeII ACGT 2 cut(s) 2227, 2963
MflI RGATCY 8 cut(s) 256, 748, 1155, 1216, 2753, 3154, 3336, 3478
MhlI GDGCHC 3 cut(s) 884, 1115, 2108
MlsI TGGCCA 1 cut(s) 1001
MluNI TGGCCA 1 cut(s) 1001
MlyI GAGTC 5 cut(s) 494, 1043, 1643, 2336, 2456
MmeI TCCRAC 1 cut(s) 1058
Mox20I TGGCCA 1 cut(s) 1001
Mph1103I ATGCAT 1 cut(s) 1635
MroXI GAANNNNTTC 3 cut(s) 289, 1668, 1788
MscI TGGCCA 1 cut(s) 1001
MslI CAYNNNNRTG 4 cut(s) 2652, 2916, 3375, 3533
Msp20I TGGCCA 1 cut(s) 1001
MspA1I CMGCKG 1 cut(s) 2636
MspI CCGG 8 cut(s) 117, 741, 1059, 1196, 2505, 2838, 3286, 3476
MspR9I CCNGG 6 cut(s) 18, 741, 1196, 2505, 2955, 3287
Mva1269I GAATGC 1 cut(s) 2927
MvaI CCWGG 2 cut(s) 18, 2955
MvnI CGCG 2 cut(s) 453, 660
MwoI GCNNNNNNNGC 7 cut(s) 1280, 2112, 2288, 2414, 2633, 2950, 2975
NciI CCSGG 4 cut(s) 741, 1196, 2505, 3287
NcoI CCATGG 2 cut(s) 411, 2025
NdeI CATATG 4 cut(s) 795, 2363, 2764, 3170
NmeAIII GCCGAG 1 cut(s) 580
NmuCI GTSAC 9 cut(s) 68, 94, 1262, 1310, 2223, 2455, 2629, 3128, 3524
NruI TCGCGA 2 cut(s) 453, 660
NsiI ATGCAT 1 cut(s) 1635
NspI RCATGY 5 cut(s) 1637, 1670, 3011, 3370, 3532
NspV TTCGAA 1 cut(s) 64
OliI CACNNNNGTG 1 cut(s) 3375
PaeI GCATGC 1 cut(s) 1637
PagI TCATGA 1 cut(s) 811
PceI AGGCCT 1 cut(s) 3415
PciI ACATGT 2 cut(s) 1666, 3528
PciSI GCTCTTC 1 cut(s) 857
PctI GAATGC 1 cut(s) 2927
PdmI GAANNNNTTC 3 cut(s) 289, 1668, 1788
PfeI GAWTC 6 cut(s) 290, 1508, 1525, 1789, 2893, 3437
Pfl23II CGTACG 1 cut(s) 3357
PflMI CCANNNNNTGG 2 cut(s) 611, 1402
PinAI ACCGGT 2 cut(s) 116, 1058
PkrI GCNGC 6 cut(s) 82, 578, 1239, 2293, 3522, 3555
Ple19I CGATCG 1 cut(s) 457
PleI GAGTC 5 cut(s) 494, 1043, 1643, 2335, 2456
PmaCI CACGTG 1 cut(s) 2228
PmlI CACGTG 1 cut(s) 2228
PpsI GAGTC 5 cut(s) 494, 1043, 1643, 2335, 2456
Ppu21I YACGTR 1 cut(s) 2228
PpuMI RGGWCCY 2 cut(s) 784, 1755
PscI ACATGT 2 cut(s) 1666, 3528
PshAI GACNNNNGTC 2 cut(s) 966, 2643
PsiI TTATAA 1 cut(s) 1344
Psp5II RGGWCCY 2 cut(s) 784, 1755
Psp6I CCWGG 2 cut(s) 16, 2953
PspCI CACGTG 1 cut(s) 2228
PspGI CCWGG 2 cut(s) 16, 2953
PspLI CGTACG 1 cut(s) 3357
PspOMI GGGCCC 1 cut(s) 2104
PspPPI RGGWCCY 2 cut(s) 784, 1755
PstI CTGCAG 2 cut(s) 147, 578
PstNI CAGNNNCTG 4 cut(s) 1598, 2431, 2594, 2615
PsuI RGATCY 8 cut(s) 256, 748, 1155, 1216, 2753, 3154, 3336, 3478
PvuI CGATCG 1 cut(s) 457
PvuII CAGCTG 1 cut(s) 2636
RruI TCGCGA 2 cut(s) 453, 660
RsaI GTAC 9 cut(s) 22, 180, 731, 861, 1641, 1679, 2164, 2761, 3359
RsaNI GTAC 9 cut(s) 21, 179, 730, 860, 1640, 1678, 2163, 2760, 3358
RseI CAYNNNNRTG 4 cut(s) 2652, 2916, 3375, 3533
SalI GTCGAC 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 857
SatI GCNGC 6 cut(s) 81, 577, 1238, 2292, 3521, 3554
ScaI AGTACT 1 cut(s) 1641
SchI GAGTC 5 cut(s) 494, 1043, 1643, 2336, 2456
ScrFI CCNGG 6 cut(s) 18, 741, 1196, 2505, 2955, 3287
SduI GDGCHC 3 cut(s) 884, 1115, 2108
SfaNI GCATC 7 cut(s) 431, 745, 1321, 1600, 2255, 2621, 2706
SfcI CTRYAG 7 cut(s) 110, 143, 574, 1084, 2184, 2607, 2973
SfuI TTCGAA 1 cut(s) 64
SinI GGWCC 3 cut(s) 784, 1249, 1755
SmiMI CAYNNNNRTG 4 cut(s) 2652, 2916, 3375, 3533
SmlI CTYRAG 1 cut(s) 1099
SmoI CTYRAG 1 cut(s) 1099
SpeI ACTAGT 2 cut(s) 3139, 3217
SphI GCATGC 1 cut(s) 1637
SseBI AGGCCT 1 cut(s) 3415
SsiI CCGC 4 cut(s) 83, 350, 1334, 3553
SspI AATATT 4 cut(s) 103, 594, 1400, 3106
SspMI CTAG 9 cut(s) 347, 375, 818, 984, 1092, 1724, 2166, 3140, 3218
StuI AGGCCT 1 cut(s) 3415
StyD4I CCNGG 6 cut(s) 16, 739, 1194, 2503, 2953, 3285
StyI CCWWGG 3 cut(s) 411, 2025, 2110
TaiI ACGT 2 cut(s) 2230, 2966
TaqI TCGA 8 cut(s) 31, 64, 425, 894, 2465, 2733, 2860, 3235
TatI WGTACW 3 cut(s) 178, 1639, 2162
TauI GCSGC 1 cut(s) 3556
TfiI GAWTC 6 cut(s) 290, 1508, 1525, 1789, 2893, 3437
TseFI GTSAC 9 cut(s) 68, 94, 1262, 1310, 2223, 2455, 2629, 3128, 3524
TseI GCWGC 5 cut(s) 80, 576, 1237, 2291, 3520
Tsp45I GTSAC 9 cut(s) 68, 94, 1262, 1310, 2223, 2455, 2629, 3128, 3524
TspGWI ACGGA 7 cut(s) 371, 658, 757, 1907, 2474, 2655, 3237
Van91I CCANNNNNTGG 2 cut(s) 611, 1402
VpaK11BI GGWCC 3 cut(s) 784, 1249, 1755
XapI RAATTY 8 cut(s) 125, 308, 566, 932, 1468, 1976, 2306, 2981
XceI RCATGY 5 cut(s) 1637, 1670, 3011, 3370, 3532
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XmiI GTMKAC 2 cut(s) 31, 109
XmnI GAANNNNTTC 3 cut(s) 289, 1668, 1788
XspI CTAG 9 cut(s) 347, 375, 818, 984, 1092, 1724, 2166, 3140, 3218
ZrmI AGTACT 1 cut(s) 1641
Zsp2I ATGCAT 1 cut(s) 1635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.