MD16G1235800.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012803
Physical Location & Seq
Reverse (-)
24503180 .. 24507863
4684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1235800.v1.1.491

Sequence Viewer

Length: 3567 bp
ATGACTGGTGAGGTTCCTGGTACGTCTGGTCGACAGTTGTGCAGAGATACAACAGATGATGTTCGAAGTGGGAGAAATGCTGCAGACGAAAATGAGACTAATGTTTGTCTACAGACTGGTGAGGAATTTTCAACGGAGTTTCTGCAGGATCGTATTGCACAAAGAAGATTAGCCCCAGTAGTGACTGGAATTGATCAACCCCAGTCGAGAAAGTTGGGTTCTAATATAAACCAACACCCTAAACCGGTTTACAAGCATCTTGGTGGTGTTATGGGGGTGAAGAGAATAGATTCCGATTGTAGTTCAGAATTCTCAGATTTTAGTCCAGTTGCTCCTAAAGGTTTTCTGGCGGACATACAGAAAAATGTTTATCCTAGTAACATGAGCAGATACTACTGGGAATATGGTTCTATGGCGCAAGCATCGGGTAAGTTTGCGGGTGAAGTTGATCGTGATCGGGTTATACCAAGACCAACTACGCCTCCTTTTTATGTTGTGGACTCCCCTCAATCATACACTCCTCATGGCCAAGTATTTGCTGATGGTTTATCTTTTAAAATGAAATTTCTCTGCAGCTTTGGGGGAAGAATATTGCCTCGGCCAAATGATGGGAAGCTCCGATATGTAGGTGGAGATACACGGATTTTATCTATTCGCAATGGCACCAATTTGGAGGAACTAATCAAGAAGACATTTGCAATTTGCAACCAACATCACACTATCAAGTACCAGCTTCCAGGTGAGGATCTTGATGCACTTGTATCTGTTTGTTCCGACGAGGACCTTCTTCATATGATAGAGGAATATCATGAGCTAGAAAGAACCGAGGGTTCTCAAAGACTGAGGCTCTTCCTTGTACCTTTATCTGAAACGGAGATCCCAAGTTCTGTCGAGGCAAGGGTAACACATCCAATCGGTGCTGACTGTGAATTTGTTTTTGCTGTTAATGGTATGTTAGACCGAAGTCCTCGGAAAAGCTCTAGCGGGCAAAGTTTGGCCAGCCAGACTAGCCAGTACGGAAATATGTCAGATTATAGTCCAACCTTTAATAGAGACTCTCCTACAGCTGCATATTTTGAGACCAAAGATTATAGCCCCAGTTCCTCAAACGCATTGGGTGCATTGCCTAAACCAGCTCCTCAGTTCTTAGCTACTCTTCAGATCCCAAAGAAGTCCTTTAATCAATCCCCTCCAATATCTCCCGTTCCACTCCAACATAGAGATCCCAAGAGTTCTAATGTACAGCTTTATTTGGATCGATCATATTGTGATGGTAGTGGAGGCATTGCTCCCTCTGCTATGGAAAAGTTTCCCTGCGACAATAGCTATTATCTTGATGCAGGGGACAGTAACGATAACCTTCATCATGAGCCTCCATTGTTAAATCACCATCATCATAACAAATATTTGGCTGAAAATTACCGGACTAAAAAGTCCCACATTGTGCACTCCCACAATCGTAGCTTTAGCGAAAATTTTGTGCCTTCTGCACAGTATGGCAAAAGTGGTATGAAATCTGAGAGACTTGTGATTCTGGAAAAGGACCTCCATTCAGAAAAATCTATCTCTCGTCCTGAAGATACAGTGGGGCTATCTTCAGGGTCTGGTGAAAGAGATGCTTCTCACCACAGAATAATGCATGCAGTACTTTCTGACCCTCAGTTGCAGGAACATGTTCAGACGACCTCCAAAGGAGAAGTTATCCCAGTGCTCTCACTGAAATATAGACTAGAAAAATCACCTTCGTTGAAGATACAAAGGTCCTCACAAGAACAGCATGTGCAACAGAAAGTGATTCTTGATGGGAAACATCAAATTGCTAAATACGGTAATCAATTCACGGCCAGGAGACCTGACCAATGTAAAGTGGAACGAGCTCTGGAGATGTTGAACCAGACAGACATGGATGACACTTGTGCTGATAAAAAATGGACACACTTTGAAGGAAATGTTGAAGTTACATCAAATGATATTGTCATGGAATTTAAGAAGGAACAAGATGCAAACTGCCTTCGCAGTGGTTGTTTAGCTTCAGAACATTCACAATGCCCTGGAAGTGGGATTTCTGGTTCATCAAACCCCAAAACTGAAGGTTCTGAAGATAATATGGGCCCCCAAGGCTATGGACTTGACACAATTTCTCAGTTTTCTCTTAGGAGCGAAAACTGTACTTGGGATCAGCGGTGTGCTATAGCTGAGACAATTAGCAGTCAACCAGTTTCTCATCGGTCCCGTGAACTTCTACCTGTTGCATCTCAGGAGTTCTTAGTCTCCACATATAAATTGGCTACAACTGCTGCACCAGACGGAGAATTTTCTCTCCATGATAAGGAACCAATGAATTATCCTAACGTAGGTCTCAGCAGACAGTCTTCTGAAGTAACCAACTGTGAAGATGCTATCCCCGCCCAGTTACAGCCACTGGATAATTGGCATGACAACAAACTGACGGACTCGGTAGTTATTGTAGAAGACTTGACTAATATGACACCATCTGGTATCCCTGCCTCTAAAGTGGTCTATCATGTCTCGCATACAGAAGATGAGGCCAGTGATGATTGTTCATCACCTGGAGAAACAGTGACCGGAAGTTTAGCTCCAGCATCTGATAACAAGGCTATGACAGCTGATGGCAGTCATAGGCATGAAACTATCAGCGACGTCGCAATAGCCGAAATGGAAGCAGGCATCTATGGTTTGCAGATCATAAACAATGATGATCTCGAAGAACTGCAAGAGTTAGGATCCGGTACATATGGAACTGTCTATCATGGCAAATGGAGAGGAACAGATGTTGCAATTAAGAGGATAAAAAAGAGTTGTTTCTCTGGAAGATCATCAGAGCAAGATCGACTGACTAAGGACTTCTGGAGAGAGGCAAAGATTCTTTCCACCCTTCACCATCCAAATGTGGTAGCATTTTATGGGGTAGTGCCTGATGGGCGTGGAGGAACGTTGGCAACTGTAGCTGAATACATGGTGAATGGATCGTTGAGGCATGCCCTAATAAGGAAAGATAGAGTGCTTGATCGTCGAAAGAGGCTCATAATCTTAATGGACACAGCTTTCGGCATGGAGTATTTGCATTTCAAAAATATTGTTCACTTTGATTTGAAGTGTGACAACTTACTTGTTAATTTGAGGGATCCCGAACGGCCCATATGCAAGGTTGGAGATTTTGGTTTGTCAAGAATTAAGCGCAACACACTAGTATCTGGTGGTGTGCGAGGAACCCTTCCGTGGATGGCACCAGAACTATTAAATGGGAGCAGCAACCGGGTTTCTGAGAAGGTTGATGTTTTCTCATTTGGTATCGTAATGTGGGAGATCCTGACTGGGGAGGAGCCGTACGCTAACATGCACTGTGGTGCCATTATTGGGGGAATTGTAAATAACACACTGAGGCCGCCTATTCCAAAGCGTTGTGATTCTGAATGGAAAAATCTAATGGAACAATGCTGGTCACCGGATCCTGCAGATCGCCCATCGTTCACGGAGATAACACATAGTCTGCGTGACATGTCAACGGCACTCCATAAAAAGCGGCCTAGTCTTGCAAGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1189

Amino Acids

132.12

Weight (kDa)

6.03

Isoelectric Point (pI)

48.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 202 - 287 5.9e-20 PB1 domain
Pkinase PF00069 909 - 1169 1e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 910 - 1171 9.4e-63 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04700 AT1G04700 AT1G04700
fragaria_vesca FvH4_4g05890
malus_domestica MD13G1231300.v1.1 MD16G1235800.v1.1
prunus_persica Prupe.1G061200_v2.0.a1
pyrus_communis pycom13g20420
rosa_chinensis RchiOBHm_Chr4g0396991
rosa_laevigata RLG00000009461
rosa_multiflora Rmu_sc0002963.1_g000016
rosa_roxburghii Rroxscaffold_5G00341980
rosa_rugosa Rorug03G0346000
rosa_samantha Rh4AG075900 Rh4BG072300 Rh4CG080600 Rh4DG069700
rosa_wichuraiana Rw4G006090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1432
AatII GACGTC 1 cut(s) 2664
AccB1I GGYRCC 3 cut(s) 662, 3247, 3368
AccB7I CCANNNNNTGG 1 cut(s) 608
AccI GTMKAC 2 cut(s) 31, 109
AciI CCGC 7 cut(s) 350, 437, 984, 2182, 2406, 3407, 3544
AclI AACGTT 1 cut(s) 2954
AcoI YGGCCR 4 cut(s) 526, 599, 996, 1842
AcsI RAATTY 7 cut(s) 125, 308, 563, 929, 1474, 1982, 2312
AcuI CTGAAG 7 cut(s) 1142, 1581, 1596, 2016, 2109, 2118, 2397
AcyI GRCGYC 1 cut(s) 2661
AdeI CACNNNGTG 1 cut(s) 1444
AfaI GTAC 9 cut(s) 22, 728, 858, 1016, 1242, 1647, 2170, 2752, 3350
AfiI CCNNNNNNNGG 9 cut(s) 244, 608, 2057, 2329, 2354, 3009, 3240, 3337, 3378
AflIII ACRYGT 2 cut(s) 1672, 3519
AgeI ACCGGT 1 cut(s) 244
AgsI TTSAA 7 cut(s) 132, 1750, 1891, 1943, 1955, 3091, 3115
AhlI ACTAGT 1 cut(s) 3208
AjnI CCWGG 5 cut(s) 16, 736, 1844, 2050, 2569
AleI CACNNNNGTG 1 cut(s) 3366
AloI GAACNNNNNNTCC 2 cut(s) 2053, 2085
Alw21I GWGCWC 3 cut(s) 1449, 1713, 1879
Alw26I GTCTC 9 cut(s) 89, 1047, 1073, 1516, 1843, 2192, 2275, 2363, 2533
Alw44I GTGCAC 1 cut(s) 1445
AlwNI CAGNNNCTG 3 cut(s) 1604, 2422, 2606
AoxI GGCC 9 cut(s) 526, 599, 996, 1842, 2110, 2547, 3155, 3404, 3545
ApaI GGGCCC 1 cut(s) 2114
ApaLI GTGCAC 1 cut(s) 1445
ApeKI GCWGC 5 cut(s) 80, 573, 1067, 2297, 3270
ApoI RAATTY 7 cut(s) 125, 308, 563, 929, 1474, 1982, 2312
ArsI GACNNNNNNTTYG 4 cut(s) 914, 946, 3050, 3082
AsiGI ACCGGT 1 cut(s) 244
Asp700I GAANNNNTTC 3 cut(s) 289, 1308, 1674
AspLEI GCGC 2 cut(s) 418, 3201
AspS9I GGNCC 7 cut(s) 781, 1543, 1761, 2110, 2111, 2229, 3156
AsuC2I CCSGG 1 cut(s) 3278
AsuII TTCGAA 1 cut(s) 64
AvaII GGWCC 4 cut(s) 781, 1543, 1761, 2229
BaeGI GKGCMC 2 cut(s) 1449, 2114
BaeI ACNNNNGTAYC 4 cut(s) 1745, 1778, 3195, 3228
BalI TGGCCA 2 cut(s) 528, 998
BamHI GGATCC 3 cut(s) 2744, 3145, 3469
BanI GGYRCC 3 cut(s) 662, 3247, 3368
BanII GRGCYC 2 cut(s) 1879, 2114
BbsI GAAGAC 3 cut(s) 695, 2364, 2478
Bbv12I GWGCWC 3 cut(s) 1449, 1713, 1879
BbvI GCAGC 5 cut(s) 67, 585, 1054, 2284, 3282
BceAI ACGGC 4 cut(s) 1857, 3170, 3331, 3543
BcgI CGANNNNNNTGC 2 cut(s) 21, 55
BciT130I CCWGG 5 cut(s) 18, 738, 1846, 2052, 2571
BciVI GTATCC 1 cut(s) 2510
BclI TGATCA 1 cut(s) 193
BcnI CCSGG 1 cut(s) 3278
BcoDI GTCTC 9 cut(s) 89, 1047, 1073, 1516, 1843, 2192, 2275, 2363, 2533
BcuI ACTAGT 1 cut(s) 3208
BfaI CTAG 7 cut(s) 375, 815, 981, 1008, 1730, 3209, 3549
BfmI CTRYAG 8 cut(s) 81, 110, 143, 571, 1062, 2190, 2964, 3474
BfuI GTATCC 1 cut(s) 2510
BglI GCCNNNNNGGC 2 cut(s) 2118, 2941
BisI GCNGC 7 cut(s) 81, 574, 1068, 2298, 3271, 3407, 3545
BlsI GCNGC 7 cut(s) 82, 575, 1069, 2299, 3272, 3408, 3546
BmcAI AGTACT 1 cut(s) 1647
Bme1390I CCNGG 6 cut(s) 18, 738, 1846, 2052, 2571, 3278
Bme18I GGWCC 4 cut(s) 781, 1543, 1761, 2229
BmgT120I GGNCC 7 cut(s) 781, 1543, 1761, 2110, 2111, 2229, 3156
BmrFI CCNGG 6 cut(s) 18, 738, 1846, 2052, 2571, 3278
BmrI ACTGGG 7 cut(s) 170, 196, 406, 1092, 1700, 2404, 3345
BmuI ACTGGG 7 cut(s) 170, 196, 406, 1092, 1700, 2404, 3345
BoxI GACNNNNGTC 1 cut(s) 963
BpiI GAAGAC 3 cut(s) 695, 2364, 2478
BpmI CTGGAG 4 cut(s) 1901, 2583, 2592, 2890
Bpu14I TTCGAA 1 cut(s) 64
BpuMI CCSGG 1 cut(s) 3278
Bsa29I ATCGAT 1 cut(s) 1258
BsaBI GATNNNNATC 1 cut(s) 453
BsaHI GRCGYC 1 cut(s) 2661
BsaI GGTCTC 3 cut(s) 1073, 1843, 2363
BsaJI CCNNGG 6 cut(s) 596, 825, 968, 2050, 2116, 3239
BsaWI WCCGGW 5 cut(s) 244, 1422, 2585, 2747, 3466
BsaXI ACNNNNNCTCC 6 cut(s) 466, 496, 2565, 2595, 3332, 3362
Bsc4I CCNNNNNNNGG 9 cut(s) 244, 608, 2057, 2329, 2354, 3009, 3240, 3337, 3378
Bse118I RCCGGY 1 cut(s) 244
Bse3DI GCAATG 3 cut(s) 664, 1121, 1284
Bse8I GATNNNNATC 1 cut(s) 453
BseBI CCWGG 5 cut(s) 18, 738, 1846, 2052, 2571
BseCI ATCGAT 1 cut(s) 1258
BseDI CCNNGG 6 cut(s) 596, 825, 968, 2050, 2116, 3239
BseGI GGATG 4 cut(s) 907, 1912, 2902, 3249
BseJI GATNNNNATC 1 cut(s) 453
BseLI CCNNNNNNNGG 9 cut(s) 244, 608, 2057, 2329, 2354, 3009, 3240, 3337, 3378
BseMI GCAATG 3 cut(s) 664, 1121, 1284
BseRI GAGGAG 3 cut(s) 510, 1128, 3356
BseSI GKGCMC 2 cut(s) 1449, 2114
BseXI GCAGC 5 cut(s) 67, 585, 1054, 2284, 3282
BsgI GTGCAG 3 cut(s) 61, 1473, 2283
BshFI GGCC 9 cut(s) 528, 601, 998, 1844, 2112, 2549, 3157, 3406, 3547
BshNI GGYRCC 3 cut(s) 662, 3247, 3368
BshTI ACCGGT 1 cut(s) 244
BshVI ATCGAT 1 cut(s) 1258
BsiHKAI GWGCWC 3 cut(s) 1449, 1713, 1879
BsiSI CCGG 6 cut(s) 245, 1423, 2586, 2748, 3277, 3467
BsiWI CGTACG 1 cut(s) 3348
BslFI GGGAC 3 cut(s) 1358, 1420, 2215
BslI CCNNNNNNNGG 9 cut(s) 244, 608, 2057, 2329, 2354, 3009, 3240, 3337, 3378
BsmAI GTCTC 9 cut(s) 89, 1047, 1073, 1516, 1843, 2192, 2275, 2363, 2533
BsmFI GGGAC 3 cut(s) 1358, 1420, 2215
BsnI GGCC 9 cut(s) 528, 601, 998, 1844, 2112, 2549, 3157, 3406, 3547
Bso31I GGTCTC 3 cut(s) 1073, 1843, 2363
Bsp119I TTCGAA 1 cut(s) 64
Bsp120I GGGCCC 1 cut(s) 2110
Bsp1286I GDGCHC 4 cut(s) 1449, 1713, 1879, 2114
Bsp1407I TGTACA 1 cut(s) 1240
BspACI CCGC 7 cut(s) 350, 437, 984, 2182, 2406, 3407, 3544
BspANI GGCC 9 cut(s) 528, 601, 998, 1844, 2112, 2549, 3157, 3406, 3547
BspDI ATCGAT 1 cut(s) 1258
BspHI TCATGA 2 cut(s) 808, 1366
BspMAI CTGCAG 4 cut(s) 85, 147, 575, 3478
BspQI GCTCTTC 1 cut(s) 854
BspT104I TTCGAA 1 cut(s) 64
BspT107I GGYRCC 3 cut(s) 662, 3247, 3368
BspTNI GGTCTC 3 cut(s) 1073, 1843, 2363
BsrDI GCAATG 3 cut(s) 664, 1121, 1284
BsrFI RCCGGY 1 cut(s) 244
BsrGI TGTACA 1 cut(s) 1240
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 6 cut(s) 596, 825, 968, 2050, 2116, 3239
BssNI GRCGYC 1 cut(s) 2661
BssT1I CCWWGG 1 cut(s) 2116
Bst2UI CCWGG 5 cut(s) 18, 738, 1846, 2052, 2571
Bst6I CTCTTC 3 cut(s) 275, 854, 1161
BstACI GRCGYC 1 cut(s) 2661
BstAPI GCANNNNNTGC 1 cut(s) 1118
BstAUI TGTACA 1 cut(s) 1240
BstBI TTCGAA 1 cut(s) 64
BstC8I GCNNGC 6 cut(s) 420, 986, 1000, 1641, 2686, 3000
BstDSI CCRYGG 1 cut(s) 3239
BstEII GGTNACC 1 cut(s) 3462
BstENI CCTNNNNNAGG 1 cut(s) 2352
BstF5I GGATG 4 cut(s) 907, 1912, 2902, 3249
BstHHI GCGC 2 cut(s) 418, 3201
BstMAI GTCTC 9 cut(s) 89, 1047, 1073, 1516, 1843, 2192, 2275, 2363, 2533
BstNI CCWGG 5 cut(s) 18, 738, 1846, 2052, 2571
BstNSI RCATGY 6 cut(s) 1643, 1676, 1781, 3002, 3361, 3523
BstPAI GACNNNNGTC 1 cut(s) 963
BstPI GGTNACC 1 cut(s) 3462
BstSCI CCNGG 6 cut(s) 16, 736, 1844, 2050, 2569, 3276
BstSFI CTRYAG 8 cut(s) 81, 110, 143, 571, 1062, 2190, 2964, 3474
BstSLI GKGCMC 2 cut(s) 1449, 2114
BstV1I GCAGC 5 cut(s) 67, 585, 1054, 2284, 3282
BstV2I GAAGAC 3 cut(s) 695, 2364, 2478
BstX2I RGATCY 8 cut(s) 745, 876, 1161, 1222, 2744, 3145, 3327, 3469
BstXI CCANNNNNNTGG 1 cut(s) 2123
BstYI RGATCY 8 cut(s) 745, 876, 1161, 1222, 2744, 3145, 3327, 3469
Bsu15I ATCGAT 1 cut(s) 1258
BsuI GTATCC 1 cut(s) 2510
BsuRI GGCC 9 cut(s) 528, 601, 998, 1844, 2112, 2549, 3157, 3406, 3547
BsuTUI ATCGAT 1 cut(s) 1258
BtgI CCRYGG 1 cut(s) 3239
BtsCI GGATG 4 cut(s) 907, 1912, 2902, 3249
BtsI GCAGTG 1 cut(s) 2023
BtsIMutI CAGTG 9 cut(s) 1590, 1713, 1715, 2023, 2420, 2557, 2586, 3361, 3398
Cac8I GCNNGC 6 cut(s) 420, 986, 1000, 1641, 2686, 3000
CaiI CAGNNNCTG 3 cut(s) 1604, 2422, 2606
CciI TCATGA 2 cut(s) 808, 1366
CfoI GCGC 2 cut(s) 418, 3201
Cfr10I RCCGGY 1 cut(s) 244
Cfr13I GGNCC 7 cut(s) 781, 1543, 1761, 2110, 2111, 2229, 3156
ClaI ATCGAT 1 cut(s) 1258
Csp6I GTAC 9 cut(s) 21, 727, 857, 1015, 1241, 1646, 2169, 2751, 3349
CspAI ACCGGT 1 cut(s) 244
CviQI GTAC 9 cut(s) 21, 727, 857, 1015, 1241, 1646, 2169, 2751, 3349
DraI TTTAAA 1 cut(s) 556
DraIII CACNNNGTG 1 cut(s) 1444
DrdI GACNNNNNNGTC 1 cut(s) 1432
DseDI GACNNNNNNGTC 1 cut(s) 1432
EaeI YGGCCR 4 cut(s) 526, 599, 996, 1842
Eam1104I CTCTTC 3 cut(s) 275, 854, 1161
EarI CTCTTC 3 cut(s) 275, 854, 1161
EciI GGCGGA 1 cut(s) 365
Ecl136II GAGCTC 1 cut(s) 1877
Eco130I CCWWGG 1 cut(s) 2116
Eco24I GRGCYC 2 cut(s) 1879, 2114
Eco31I GGTCTC 3 cut(s) 1073, 1843, 2363
Eco47I GGWCC 4 cut(s) 781, 1543, 1761, 2229
Eco53kI GAGCTC 1 cut(s) 1877
Eco57I CTGAAG 7 cut(s) 1142, 1581, 1596, 2016, 2109, 2118, 2397
Eco91I GGTNACC 1 cut(s) 3462
EcoICRI GAGCTC 1 cut(s) 1877
EcoNI CCTNNNNNAGG 1 cut(s) 2352
EcoO109I RGGNCCY 4 cut(s) 781, 1543, 1761, 2111
EcoO65I GGTNACC 1 cut(s) 3462
EcoRI GAATTC 1 cut(s) 308
EcoRII CCWGG 5 cut(s) 16, 736, 1844, 2050, 2569
EcoT14I CCWWGG 1 cut(s) 2116
EcoT22I ATGCAT 1 cut(s) 1641
EcoT38I GRGCYC 2 cut(s) 1879, 2114
ErhI CCWWGG 1 cut(s) 2116
FalI AAGNNNNNCTT 6 cut(s) 1160, 1192, 1603, 1635, 1782, 1814
FaqI GGGAC 3 cut(s) 1358, 1420, 2215
FauI CCCGC 3 cut(s) 430, 977, 2413
FauNDI CATATG 3 cut(s) 792, 2755, 3161
FbaI TGATCA 1 cut(s) 193
FblI GTMKAC 2 cut(s) 31, 109
Fnu4HI GCNGC 7 cut(s) 81, 574, 1068, 2298, 3271, 3407, 3545
FokI GGATG 4 cut(s) 894, 1919, 2889, 3256
FriOI GRGCYC 2 cut(s) 1879, 2114
Fsp4HI GCNGC 7 cut(s) 81, 574, 1068, 2298, 3271, 3407, 3545
FspBI CTAG 7 cut(s) 375, 815, 981, 1008, 1730, 3209, 3549
GlaI GCGC 2 cut(s) 417, 3200
GluI GCNGC 7 cut(s) 81, 574, 1068, 2298, 3271, 3407, 3545
GsuI CTGGAG 4 cut(s) 1901, 2583, 2592, 2890
HaeIII GGCC 9 cut(s) 528, 601, 998, 1844, 2112, 2549, 3157, 3406, 3547
HapII CCGG 6 cut(s) 245, 1423, 2586, 2748, 3277, 3467
HhaI GCGC 2 cut(s) 418, 3201
Hin1I GRCGYC 1 cut(s) 2661
Hin6I GCGC 2 cut(s) 416, 3199
HinP1I GCGC 2 cut(s) 416, 3199
HincII GTYRAC 3 cut(s) 32, 2213, 3525
HindII GTYRAC 3 cut(s) 32, 2213, 3525
HinfI GANTC 8 cut(s) 290, 500, 1055, 1531, 1795, 2453, 2884, 3428
HpaII CCGG 6 cut(s) 245, 1423, 2586, 2748, 3277, 3467
Hpy99I CGWCG 4 cut(s) 779, 2663, 2666, 3036
HpyCH4IV ACGT 4 cut(s) 23, 2352, 2661, 2954
HpySE526I ACGT 4 cut(s) 23, 2352, 2661, 2954
Hsp92I GRCGYC 1 cut(s) 2661
HspAI GCGC 2 cut(s) 416, 3199
Ksp22I TGATCA 1 cut(s) 193
LguI GCTCTTC 1 cut(s) 854
LmnI GCTCC 8 cut(s) 337, 621, 1141, 1294, 2157, 2602, 3267, 3343
Lsp1109I GCAGC 5 cut(s) 67, 585, 1054, 2284, 3282
MaeI CTAG 7 cut(s) 375, 815, 981, 1008, 1730, 3209, 3549
MaeII ACGT 4 cut(s) 23, 2352, 2661, 2954
MflI RGATCY 8 cut(s) 745, 876, 1161, 1222, 2744, 3145, 3327, 3469
MhlI GDGCHC 4 cut(s) 1449, 1713, 1879, 2114
MlsI TGGCCA 2 cut(s) 528, 998
MluNI TGGCCA 2 cut(s) 528, 998
MlyI GAGTC 3 cut(s) 494, 1049, 2447
MmeI TCCRAC 4 cut(s) 798, 1064, 1237, 3151
Mox20I TGGCCA 2 cut(s) 528, 998
Mph1103I ATGCAT 1 cut(s) 1641
MroXI GAANNNNTTC 3 cut(s) 289, 1308, 1674
MscI TGGCCA 2 cut(s) 528, 998
MslI CAYNNNNRTG 4 cut(s) 261, 2643, 2907, 3366
Msp20I TGGCCA 2 cut(s) 528, 998
MspA1I CMGCKG 3 cut(s) 1067, 2182, 2627
MspI CCGG 6 cut(s) 245, 1423, 2586, 2748, 3277, 3467
MspR9I CCNGG 6 cut(s) 18, 738, 1846, 2052, 2571, 3278
MvaI CCWGG 5 cut(s) 18, 738, 1846, 2052, 2571
NciI CCSGG 1 cut(s) 3278
NdeI CATATG 3 cut(s) 792, 2755, 3161
NmeAIII GCCGAG 1 cut(s) 577
NmuCI GTSAC 5 cut(s) 181, 2581, 3119, 3462, 3515
NsiI ATGCAT 1 cut(s) 1641
NspI RCATGY 6 cut(s) 1643, 1676, 1781, 3002, 3361, 3523
NspV TTCGAA 1 cut(s) 64
OliI CACNNNNGTG 1 cut(s) 3366
PaeI GCATGC 2 cut(s) 1643, 3002
PagI TCATGA 2 cut(s) 808, 1366
PciI ACATGT 2 cut(s) 1672, 3519
PciSI GCTCTTC 1 cut(s) 854
PcsI WCGNNNNNNNCGW 1 cut(s) 2670
PdmI GAANNNNTTC 3 cut(s) 289, 1308, 1674
PfeI GAWTC 5 cut(s) 290, 1531, 1795, 2884, 3428
Pfl23II CGTACG 1 cut(s) 3348
PflMI CCANNNNNTGG 1 cut(s) 608
PinAI ACCGGT 1 cut(s) 244
PkrI GCNGC 7 cut(s) 82, 575, 1069, 2299, 3272, 3408, 3546
PleI GAGTC 3 cut(s) 494, 1049, 2447
PpsI GAGTC 3 cut(s) 494, 1049, 2447
PpuMI RGGWCCY 3 cut(s) 781, 1543, 1761
PscI ACATGT 2 cut(s) 1672, 3519
PshAI GACNNNNGTC 1 cut(s) 963
Psp124BI GAGCTC 1 cut(s) 1879
Psp1406I AACGTT 1 cut(s) 2954
Psp5II RGGWCCY 3 cut(s) 781, 1543, 1761
Psp6I CCWGG 5 cut(s) 16, 736, 1844, 2050, 2569
PspEI GGTNACC 1 cut(s) 3462
PspGI CCWGG 5 cut(s) 16, 736, 1844, 2050, 2569
PspLI CGTACG 1 cut(s) 3348
PspOMI GGGCCC 1 cut(s) 2110
PspPI GGNCC 7 cut(s) 781, 1543, 1761, 2110, 2111, 2229, 3156
PspPPI RGGWCCY 3 cut(s) 781, 1543, 1761
PstI CTGCAG 4 cut(s) 85, 147, 575, 3478
PstNI CAGNNNCTG 3 cut(s) 1604, 2422, 2606
PsuI RGATCY 8 cut(s) 745, 876, 1161, 1222, 2744, 3145, 3327, 3469
PvuII CAGCTG 2 cut(s) 1067, 2627
RsaI GTAC 9 cut(s) 22, 728, 858, 1016, 1242, 1647, 2170, 2752, 3350
RsaNI GTAC 9 cut(s) 21, 727, 857, 1015, 1241, 1646, 2169, 2751, 3349
RseI CAYNNNNRTG 4 cut(s) 261, 2643, 2907, 3366
SacI GAGCTC 1 cut(s) 1879
SalI GTCGAC 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 854
SatI GCNGC 7 cut(s) 81, 574, 1068, 2298, 3271, 3407, 3545
Sau96I GGNCC 7 cut(s) 781, 1543, 1761, 2110, 2111, 2229, 3156
ScaI AGTACT 1 cut(s) 1647
SchI GAGTC 3 cut(s) 494, 1049, 2447
ScrFI CCNGG 6 cut(s) 18, 738, 1846, 2052, 2571, 3278
SduI GDGCHC 4 cut(s) 1449, 1713, 1879, 2114
SfcI CTRYAG 8 cut(s) 81, 110, 143, 571, 1062, 2190, 2964, 3474
SfuI TTCGAA 1 cut(s) 64
SinI GGWCC 4 cut(s) 781, 1543, 1761, 2229
SmiMI CAYNNNNRTG 4 cut(s) 261, 2643, 2907, 3366
SpeI ACTAGT 1 cut(s) 3208
SphI GCATGC 2 cut(s) 1643, 3002
SsiI CCGC 7 cut(s) 350, 437, 984, 2182, 2406, 3407, 3544
SspI AATATT 3 cut(s) 591, 1406, 3097
SspMI CTAG 7 cut(s) 375, 815, 981, 1008, 1730, 3209, 3549
SstI GAGCTC 1 cut(s) 1879
StyD4I CCNGG 6 cut(s) 16, 736, 1844, 2050, 2569, 3276
StyI CCWWGG 1 cut(s) 2116
TaiI ACGT 4 cut(s) 26, 2355, 2664, 2957
TaqI TCGA 8 cut(s) 31, 64, 206, 891, 1258, 2724, 2851, 3034
TaqII GACCGA 2 cut(s) 975, 2217
TatI WGTACW 3 cut(s) 1240, 1645, 2168
TauI GCSGC 2 cut(s) 3409, 3547
TfiI GAWTC 5 cut(s) 290, 1531, 1795, 2884, 3428
TscAI CASTG 9 cut(s) 1590, 1713, 1722, 2023, 2427, 2557, 2586, 3368, 3405
TseFI GTSAC 5 cut(s) 181, 2581, 3119, 3462, 3515
TseI GCWGC 5 cut(s) 80, 573, 1067, 2297, 3270
Tsp45I GTSAC 5 cut(s) 181, 2581, 3119, 3462, 3515
TspDTI ATGAA 8 cut(s) 575, 779, 1352, 1526, 2061, 2354, 2553, 2661
TspGWI ACGGA 8 cut(s) 149, 655, 887, 1032, 2322, 2465, 3228, 3509
TspRI CASTG 9 cut(s) 1590, 1713, 1722, 2023, 2427, 2557, 2586, 3368, 3405
Van91I CCANNNNNTGG 1 cut(s) 608
VneI GTGCAC 1 cut(s) 1445
VpaK11BI GGWCC 4 cut(s) 781, 1543, 1761, 2229
XagI CCTNNNNNAGG 1 cut(s) 2352
XapI RAATTY 7 cut(s) 125, 308, 563, 929, 1474, 1982, 2312
XceI RCATGY 6 cut(s) 1643, 1676, 1781, 3002, 3361, 3523
XcmI CCANNNNNNNNNTGG 2 cut(s) 2281, 2427
XmiI GTMKAC 2 cut(s) 31, 109
XmnI GAANNNNTTC 3 cut(s) 289, 1308, 1674
XspI CTAG 7 cut(s) 375, 815, 981, 1008, 1730, 3209, 3549
ZraI GACGTC 1 cut(s) 2662
ZrmI AGTACT 1 cut(s) 1647
Zsp2I ATGCAT 1 cut(s) 1641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.