Rmu_sc0002963.1_g000016

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002963.1
OG0012803
Physical Location & Seq
Reverse (-)
103216 .. 108291
5076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002963.1_g000016.1.cds

Sequence Viewer

Length: 3558 bp
atgactagtgaggttcgtggtacttctggactacagttccgcaaagatgcaacaaatcatgtgccaagtaacacaagtggtgcagatagagatgtagataatatttgtgtacagacgggcgaggaattctcaacggagtttctgcaggatcgtatagcacaaagaagattagccccagtagtgactggtgttgagcagcaccagtcgaaaagagtgggatttaatttaaatcaaaacaatttgcagctcgttaatgaggatctaaatggaattcttggattaaggaggttagattctgatggaagttcagaattttcagattttgttacagcgaaaagagtgggatttaatttaaatcaaaacaatttgcagctcgttaatgaggatctaaatggaattcttggattaaggagattagattctgatggaagttcagaattttcagattttgttacagctggtagtcctggtacaggttatgtgcctgatttagagaagaatgtttttcctagtagtaatgtcagcagacagtatggtggtcttcgaagacaactatcaggtaagtttgcggatgaagttaatcgtgattgggttattcctagatcaaatactccacctttttatgttggtgaatcccctcagtcatatagtccatatggtcaaggattttttgaaggctctttctctttcaaaatgaagtttctctgcagttttggggggagaatattgcctaggccaaatgatgggaagctcagatatgttggtggagatacacggattttatctattcacaaaggcaccaatttcgaggaactaatgaagaagacattggcaatttgtaaccacccccacactatcaagtaccaacttcctggtgaggagcttgatgcacttgtatctgtttgttctgatgaggacctccatcacatgatagaggaatatcatgagctggaaagaactgagggttctcaaagattgcggctctttcttgtacctttaaatgaaccagagtgccctagttctattgaaggcaggatcacacatccaatggggcctgacagtcaattcgtctttgctgtcaatggcatgctagacagaagtcctcggaaaagctccagcggtcagagtttgacaagtcagaatagtcactttgggaatgcctcagattatagtccaacttttcatagagacacttccacatctgcatttactttagagaacaaggatgttagtctaagttcctcaaatctgtcggggacaagtagtaaacatgctcgccagttcttaggtactcttcagatcccaccgaagtcttttattccatcccctccactgtctcctgttcaacttcaacatagggacctcaaaaattctaatgtgcagctacatttggatcaaccatattgtcatggtaatggaggcattgctctctctgatgttgatgttgtgggctatatcaataatcttgatcatggtcctccattgatgaattaccatcagcacaataaaaatttggctgagacctacctgactaaaaaatcccacaacatgccctccctcaatcgcagcgcaagtgaagattttgtgccttctgccctgtatggtcaaggtggcatgaactttgagagacacatgcctcttagaaagcccttgatccattctgacaagtctgtctctcatcctaaagatatactggagttattgttaccgactggtaatatagatgcctctcatcaaaaaataatgcatgtactttctgactctcagttgcacgagcatctcgaaaggtccttggaaggagaaaatgtctcagtaccattgaatttgatcagagaaggatcaccttcactggagatgccaagttcctcgcaagaacaagaggctcagttggttgaccagaaaaacaaaatggttacacatcaatttaacatcagcacacctcaccattgcaaggaggaagaaggtctggaggtgttcaaccattacacgaatgacacttctgctgataaaaagtggaagcacctcgaaggaaatgttgaagttacttcaaatgatgctgtcatggaatttaagaacttactaaatatgaactaccttaaagatgcagtggatccccaagagtacaaattagacacaacttctcagtattctcataggattcaaaatggtacaaatgatcagcagtgtactacaacaattagtggtcaatcaatttcccggcctcatggattactaactgttgcatctcaagaattcttaatctcaagccctcaaatgcctacaagttctgcttcagatagagaatattctctgcatgataactatcccgagtatgtggttgaaaaggtgggttctggcagacactctttcgagaatctgaaatgtgaagatgttcgccctgtccagtcacagcccttagataatcataatgacaataaggtgacaaaatcagtggttattgttgaagacttaactaatagcacgccacctggaattcccgcttctaaagcggtctatcgtgtctcccatatagaagatgagaccagtgatgaatgttcatcacctacagagacagagataagtgcagctccagagtctgagagaaagggtgtgactgcagatggcaaagataggcatgaaactattagtgatgctgtaatagccgaaatggaagcaggcatctatggtttgcagatcataaaaaatgctgatcttgaagaactgcaagagttgggatctggtacatttggaactgtctaccatgggaagtggagaggaacagatgttgctattaaaagaataaaaaagagttgtttttctgacagatcatcagagcaagagcggctgactaaggacttctggagagaggcaaagattctttccacccttcaccatccaaatgtagtagcactttatggagtagtacctgatggccctggaggaacattggcaactgtagctgaatttatggtgaatggatcattgaggcatgtcctagttaggaaggacagagtacttgaccgccgaaagaggctcataatcttgatggacacagcttttggcatggaatatttgcatttgaaaaatattgttcactttgatttgaagtgtgacaatttgcttgtcaatttgagggatcctgaaagacccatatgtaaggttgctgattttgggctgtcaagaattaaacgcaacacactagtatctggtggtgtgcgaggaacccttccatggatggcaccagaattattaaatggtagcagtagccgtgtctctgagaaggttgatgtcttctcatttggtattgtaatgtgggagatcttgactggggaggaaccgtatgccaacatgcattgtggtgctatcatagggggaattgtaaataatacactaaggcctcctattccaaagcgctgcgactcggagtggaaaaagttaatggaagagtgctggtcgccggatcctgctgatcggccatcattcacagatataacacataggctacgtgatatgtcaacggcactccagaagaagcggtctaatgttgcaagtagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1185

Amino Acids

132.28

Weight (kDa)

5.92

Isoelectric Point (pI)

50.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04700 AT1G04700 AT1G04700
fragaria_vesca FvH4_4g05890
malus_domestica MD13G1231300.v1.1 MD16G1235800.v1.1
prunus_persica Prupe.1G061200_v2.0.a1
pyrus_communis pycom13g20420
rosa_chinensis RchiOBHm_Chr4g0396991
rosa_laevigata RLG00000009461
rosa_multiflora Rmu_sc0002963.1_g000016
rosa_roxburghii Rroxscaffold_5G00341980
rosa_rugosa Rorug03G0346000
rosa_samantha Rh4AG075900 Rh4BG072300 Rh4CG080600 Rh4DG069700
rosa_wichuraiana Rw4G006090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1670
AccB1I GGYRCC 2 cut(s) 797, 3238
AccB7I CCANNNNNTGG 1 cut(s) 743
AccBSI CCGCTC 1 cut(s) 2842
AccI GTMKAC 1 cut(s) 2757
AciI CCGC 9 cut(s) 40, 569, 979, 1119, 2499, 2510, 2842, 3022, 3535
AcoI YGGCCR 1 cut(s) 3473
AcuI CTGAAG 2 cut(s) 1280, 2277
AfeI AGCGCT 1 cut(s) 3413
AfiI CCNNNNNNNGG 4 cut(s) 473, 743, 1951, 3231
AhlI ACTAGT 2 cut(s) 5, 3199
AjnI CCWGG 4 cut(s) 466, 871, 2488, 2935
AjuI GAANNNNNNNTTGG 2 cut(s) 812, 844
AlwNI CAGNNNCTG 1 cut(s) 2597
Ama87I CYCGRG 1 cut(s) 2327
Aor51HI AGCGCT 1 cut(s) 3413
AoxI GGCC 6 cut(s) 734, 1052, 2219, 2932, 3395, 3473
ApeKI GCWGC 7 cut(s) 196, 244, 370, 1381, 1566, 2585, 3414
ArsI GACNNNNNNTTYG 4 cut(s) 1049, 1081, 3041, 3073
Asp700I GAANNNNTTC 1 cut(s) 2391
AspA2I CCTAGG 1 cut(s) 731
AspLEI GCGC 2 cut(s) 1571, 3414
AspS9I GGNCC 6 cut(s) 916, 1052, 1360, 1475, 1788, 2933
AsuC2I CCSGG 1 cut(s) 2218
AsuHPI GGTGA 8 cut(s) 641, 887, 1833, 1934, 2452, 2553, 2882, 2983
AsuII TTCGAA 1 cut(s) 544
AvaI CYCGRG 1 cut(s) 2327
AvaII GGWCC 4 cut(s) 916, 1360, 1475, 1788
AvrII CCTAGG 1 cut(s) 731
BaeGI GKGCMC 1 cut(s) 1016
BaeI ACNNNNGTAYC 2 cut(s) 3186, 3219
BamHI GGATCC 3 cut(s) 2110, 3136, 3460
BanI GGYRCC 2 cut(s) 797, 3238
BauI CACGAG 1 cut(s) 1772
BbsI GAAGAC 5 cut(s) 533, 553, 830, 2472, 3283
BbvI GCAGC 7 cut(s) 208, 256, 382, 1393, 1578, 2597, 3401
BceAI ACGGC 2 cut(s) 3252, 3534
BcgI CGANNNNNNTGC 2 cut(s) 787, 821
BciT130I CCWGG 4 cut(s) 468, 873, 2490, 2937
BclI TGATCA 3 cut(s) 1468, 1827, 2176
BcnI CCSGG 1 cut(s) 2218
BcuI ACTAGT 2 cut(s) 5, 3199
BfaI CTAG 8 cut(s) 6, 510, 600, 732, 1017, 1091, 2996, 3200
BfmI CTRYAG 6 cut(s) 32, 143, 706, 2565, 2616, 2955
BfoI RGCGCY 1 cut(s) 3415
BglII AGATCT 1 cut(s) 3318
BisI GCNGC 9 cut(s) 197, 245, 371, 980, 1382, 1567, 2586, 2843, 3415
BlnI CCTAGG 1 cut(s) 731
BlsI GCNGC 9 cut(s) 198, 246, 372, 981, 1383, 1568, 2587, 2844, 3416
BmcAI AGTACT 1 cut(s) 3015
Bme1390I CCNGG 5 cut(s) 468, 873, 2218, 2490, 2937
Bme18I GGWCC 4 cut(s) 916, 1360, 1475, 1788
BmeT110I CYCGRG 1 cut(s) 2327
BmgT120I GGNCC 6 cut(s) 916, 1052, 1360, 1475, 1788, 2933
BmiI GGNNCC 9 cut(s) 799, 1053, 1361, 2112, 3138, 3223, 3240, 3336, 3462
BmrFI CCNGG 5 cut(s) 468, 873, 2218, 2490, 2937
BmrI ACTGGG 2 cut(s) 170, 3336
BmuI ACTGGG 2 cut(s) 170, 3336
BoxI GACNNNNGTC 1 cut(s) 1098
BpiI GAAGAC 5 cut(s) 533, 553, 830, 2472, 3283
BplI GAGNNNNNCTC 2 cut(s) 113, 145
BpmI CTGGAG 8 cut(s) 1099, 1715, 1871, 1988, 2574, 2881, 2958, 3509
Bpu14I TTCGAA 1 cut(s) 544
BpuEI CTTGAG 2 cut(s) 2232, 2248
BpuMI CCSGG 1 cut(s) 2218
BsaAI YACGTR 1 cut(s) 3506
BsaBI GATNNNNATC 1 cut(s) 2322
BsaI GGTCTC 2 cut(s) 1514, 2534
BsaJI CCNNGG 6 cut(s) 731, 1103, 1791, 2761, 2935, 3230
BsaXI ACNNNNNCTCC 8 cut(s) 1538, 1568, 1688, 1718, 1791, 1821, 3323, 3353
Bsc4I CCNNNNNNNGG 4 cut(s) 473, 743, 1951, 3231
Bse3DI GCAATG 2 cut(s) 1422, 1945
Bse8I GATNNNNATC 1 cut(s) 2322
BseBI CCWGG 4 cut(s) 468, 873, 2490, 2937
BseDI CCNNGG 6 cut(s) 731, 1103, 1791, 2761, 2935, 3230
BseGI GGATG 7 cut(s) 577, 1042, 1231, 1322, 1678, 2893, 3240
BseJI GATNNNNATC 1 cut(s) 2322
BseLI CCNNNNNNNGG 4 cut(s) 473, 743, 1951, 3231
BseMI GCAATG 2 cut(s) 1422, 1945
BseRI GAGGAG 1 cut(s) 893
BseSI GKGCMC 1 cut(s) 1016
BseXI GCAGC 7 cut(s) 208, 256, 382, 1393, 1578, 2597, 3401
BsgI GTGCAG 3 cut(s) 102, 1400, 2604
BshFI GGCC 6 cut(s) 736, 1054, 2221, 2934, 3397, 3475
BshNI GGYRCC 2 cut(s) 797, 3238
BsiHKCI CYCGRG 1 cut(s) 2327
BsiSI CCGG 2 cut(s) 2218, 3458
BslFI GGGAC 2 cut(s) 1270, 1373
BslI CCNNNNNNNGG 4 cut(s) 473, 743, 1951, 3231
BsmFI GGGAC 2 cut(s) 1270, 1373
BsmI GAATGC 1 cut(s) 1162
BsnI GGCC 6 cut(s) 736, 1054, 2221, 2934, 3397, 3475
Bso31I GGTCTC 2 cut(s) 1514, 2534
BsoBI CYCGRG 1 cut(s) 2327
Bsp119I TTCGAA 1 cut(s) 544
Bsp1286I GDGCHC 1 cut(s) 1016
Bsp1407I TGTACA 1 cut(s) 109
Bsp19I CCATGG 2 cut(s) 2761, 3230
BspACI CCGC 9 cut(s) 40, 569, 979, 1119, 2499, 2510, 2842, 3022, 3535
BspANI GGCC 6 cut(s) 736, 1054, 2221, 2934, 3397, 3475
BspHI TCATGA 1 cut(s) 943
BspLI GGNNCC 9 cut(s) 799, 1053, 1361, 2112, 3138, 3223, 3240, 3336, 3462
BspMAI CTGCAG 3 cut(s) 147, 710, 2620
BspT104I TTCGAA 1 cut(s) 544
BspT107I GGYRCC 2 cut(s) 797, 3238
BspTNI GGTCTC 2 cut(s) 1514, 2534
BsrBI CCGCTC 1 cut(s) 2842
BsrDI GCAATG 2 cut(s) 1422, 1945
BsrGI TGTACA 1 cut(s) 109
BssECI CCNNGG 6 cut(s) 731, 1103, 1791, 2761, 2935, 3230
BssSI CACGAG 1 cut(s) 1772
BssT1I CCWWGG 4 cut(s) 731, 1791, 2761, 3230
Bst2BI CACGAG 1 cut(s) 1772
Bst2UI CCWGG 4 cut(s) 468, 873, 2490, 2937
Bst4CI ACNGT 8 cut(s) 36, 531, 1061, 1335, 2239, 2755, 2956, 3339
Bst6I CTCTTC 2 cut(s) 1299, 3438
BstAUI TGTACA 1 cut(s) 109
BstBAI YACGTR 1 cut(s) 3506
BstBI TTCGAA 1 cut(s) 544
BstC8I GCNNGC 4 cut(s) 1088, 1276, 2483, 2677
BstDSI CCRYGG 2 cut(s) 2761, 3230
BstENI CCTNNNNNAGG 1 cut(s) 471
BstF5I GGATG 7 cut(s) 577, 1042, 1231, 1322, 1678, 2893, 3240
BstH2I RGCGCY 1 cut(s) 3415
BstHHI GCGC 2 cut(s) 1571, 3414
BstMWI GCNNNNNNNGC 4 cut(s) 2507, 2660, 2842, 2957
BstNI CCWGG 4 cut(s) 468, 873, 2490, 2937
BstNSI RCATGY 7 cut(s) 1090, 1274, 1552, 1636, 1751, 2993, 3352
BstPAI GACNNNNGTC 1 cut(s) 1098
BstSCI CCNGG 5 cut(s) 466, 871, 2216, 2488, 2935
BstSFI CTRYAG 6 cut(s) 32, 143, 706, 2565, 2616, 2955
BstSLI GKGCMC 1 cut(s) 1016
BstV1I GCAGC 7 cut(s) 208, 256, 382, 1393, 1578, 2597, 3401
BstV2I GAAGAC 5 cut(s) 533, 553, 830, 2472, 3283
BstX2I RGATCY 8 cut(s) 259, 385, 1299, 2110, 2735, 3136, 3318, 3460
BstXI CCANNNNNNTGG 1 cut(s) 872
BstYI RGATCY 8 cut(s) 259, 385, 1299, 2110, 2735, 3136, 3318, 3460
BsuRI GGCC 6 cut(s) 736, 1054, 2221, 2934, 3397, 3475
BtgI CCRYGG 2 cut(s) 2761, 3230
BtsCI GGATG 7 cut(s) 577, 1042, 1231, 1322, 1678, 2893, 3240
BtsI GCAGTG 2 cut(s) 2112, 2189
BtsIMutI CAGTG 6 cut(s) 1331, 1847, 2112, 2189, 2457, 2551
Cac8I GCNNGC 4 cut(s) 1088, 1276, 2483, 2677
CaiI CAGNNNCTG 1 cut(s) 2597
CciI TCATGA 1 cut(s) 943
CfoI GCGC 2 cut(s) 1571, 3414
Cfr13I GGNCC 6 cut(s) 916, 1052, 1360, 1475, 1788, 2933
CspCI CAANNNNNGTGG 2 cut(s) 2433, 2468
DraI TTTAAA 3 cut(s) 228, 354, 999
DrdI GACNNNNNNGTC 1 cut(s) 1670
DseDI GACNNNNNNGTC 1 cut(s) 1670
EaeI YGGCCR 1 cut(s) 3473
Eam1104I CTCTTC 2 cut(s) 1299, 3438
EarI CTCTTC 2 cut(s) 1299, 3438
Eco130I CCWWGG 4 cut(s) 731, 1791, 2761, 3230
Eco147I AGGCCT 1 cut(s) 3397
Eco31I GGTCTC 2 cut(s) 1514, 2534
Eco47I GGWCC 4 cut(s) 916, 1360, 1475, 1788
Eco47III AGCGCT 1 cut(s) 3413
Eco57I CTGAAG 2 cut(s) 1280, 2277
Eco88I CYCGRG 1 cut(s) 2327
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoO109I RGGNCCY 4 cut(s) 916, 1052, 1360, 1788
EcoRI GAATTC 5 cut(s) 125, 270, 396, 2252, 2493
EcoRII CCWGG 4 cut(s) 466, 871, 2488, 2935
EcoT14I CCWWGG 4 cut(s) 731, 1791, 2761, 3230
EcoT22I ATGCAT 2 cut(s) 1749, 3354
ErhI CCWWGG 4 cut(s) 731, 1791, 2761, 3230
FalI AAGNNNNNCTT 2 cut(s) 2275, 2307
FaqI GGGAC 2 cut(s) 1270, 1373
FauI CCCGC 1 cut(s) 2506
FauNDI CATATG 2 cut(s) 655, 3152
FbaI TGATCA 3 cut(s) 1468, 1827, 2176
FblI GTMKAC 1 cut(s) 2757
Fnu4HI GCNGC 9 cut(s) 197, 245, 371, 980, 1382, 1567, 2586, 2843, 3415
FokI GGATG 7 cut(s) 584, 1029, 1238, 1309, 1665, 2880, 3247
Fsp4HI GCNGC 9 cut(s) 197, 245, 371, 980, 1382, 1567, 2586, 2843, 3415
FspBI CTAG 8 cut(s) 6, 510, 600, 732, 1017, 1091, 2996, 3200
GlaI GCGC 2 cut(s) 1570, 3413
GluI GCNGC 9 cut(s) 197, 245, 371, 980, 1382, 1567, 2586, 2843, 3415
GsuI CTGGAG 8 cut(s) 1099, 1715, 1871, 1988, 2574, 2881, 2958, 3509
HaeII RGCGCY 1 cut(s) 3415
HaeIII GGCC 6 cut(s) 736, 1054, 2221, 2934, 3397, 3475
HapII CCGG 2 cut(s) 2218, 3458
HhaI GCGC 2 cut(s) 1571, 3414
Hin6I GCGC 2 cut(s) 1569, 3412
HinP1I GCGC 2 cut(s) 1569, 3412
HincII GTYRAC 2 cut(s) 1894, 3516
HindII GTYRAC 2 cut(s) 1894, 3516
HinfI GANTC 9 cut(s) 293, 419, 632, 1760, 2158, 2374, 2594, 2875, 3419
HpaII CCGG 2 cut(s) 2218, 3458
HphI GGTGA 8 cut(s) 641, 887, 1833, 1934, 2452, 2553, 2882, 2983
Hpy166II GTNNAC 7 cut(s) 110, 1268, 1894, 2187, 2758, 3094, 3516
Hpy8I GTNNAC 7 cut(s) 110, 1268, 1894, 2187, 2758, 3094, 3516
HpyCH4III ACNGT 8 cut(s) 36, 531, 1061, 1335, 2239, 2755, 2956, 3339
HpyCH4IV ACGT 1 cut(s) 3505
HpyF10VI GCNNNNNNNGC 4 cut(s) 2507, 2660, 2842, 2957
HpySE526I ACGT 1 cut(s) 3505
HspAI GCGC 2 cut(s) 1569, 3412
Ksp22I TGATCA 3 cut(s) 1468, 1827, 2176
LmnI GCTCC 3 cut(s) 880, 1118, 2593
Lsp1109I GCAGC 7 cut(s) 208, 256, 382, 1393, 1578, 2597, 3401
MaeI CTAG 8 cut(s) 6, 510, 600, 732, 1017, 1091, 2996, 3200
MaeII ACGT 1 cut(s) 3505
MbiI CCGCTC 1 cut(s) 2842
MflI RGATCY 8 cut(s) 259, 385, 1299, 2110, 2735, 3136, 3318, 3460
MhlI GDGCHC 1 cut(s) 1016
MlyI GAGTC 3 cut(s) 1754, 2603, 3413
MmeI TCCRAC 1 cut(s) 1199
Mph1103I ATGCAT 2 cut(s) 1749, 3354
MroXI GAANNNNTTC 1 cut(s) 2391
MslI CAYNNNNRTG 3 cut(s) 1413, 2898, 3357
MspA1I CMGCKG 2 cut(s) 458, 1119
MspI CCGG 2 cut(s) 2218, 3458
MspR9I CCNGG 5 cut(s) 468, 873, 2218, 2490, 2937
Mva1269I GAATGC 1 cut(s) 1162
MvaI CCWGG 4 cut(s) 468, 873, 2490, 2937
MwoI GCNNNNNNNGC 4 cut(s) 2507, 2660, 2842, 2957
NciI CCSGG 1 cut(s) 2218
NcoI CCATGG 2 cut(s) 2761, 3230
NdeI CATATG 2 cut(s) 655, 3152
NlaIV GGNNCC 9 cut(s) 799, 1053, 1361, 2112, 3138, 3223, 3240, 3336, 3462
NmuCI GTSAC 6 cut(s) 181, 1145, 2406, 2440, 2611, 3110
NsiI ATGCAT 2 cut(s) 1749, 3354
NspI RCATGY 7 cut(s) 1090, 1274, 1552, 1636, 1751, 2993, 3352
NspV TTCGAA 1 cut(s) 544
PaeI GCATGC 1 cut(s) 1090
PagI TCATGA 1 cut(s) 943
PceI AGGCCT 1 cut(s) 3397
PcsI WCGNNNNNNNCGW 1 cut(s) 1779
PctI GAATGC 1 cut(s) 1162
PdmI GAANNNNTTC 1 cut(s) 2391
PfeI GAWTC 6 cut(s) 293, 419, 632, 2158, 2374, 2875
PflMI CCANNNNNTGG 1 cut(s) 743
PkrI GCNGC 9 cut(s) 198, 246, 372, 981, 1383, 1568, 2587, 2844, 3416
PleI GAGTC 3 cut(s) 1754, 2602, 3413
PpsI GAGTC 3 cut(s) 1754, 2602, 3413
Ppu21I YACGTR 1 cut(s) 3506
PpuMI RGGWCCY 3 cut(s) 916, 1360, 1788
PshAI GACNNNNGTC 1 cut(s) 1098
Psp5II RGGWCCY 3 cut(s) 916, 1360, 1788
Psp6I CCWGG 4 cut(s) 466, 871, 2488, 2935
PspGI CCWGG 4 cut(s) 466, 871, 2488, 2935
PspN4I GGNNCC 9 cut(s) 799, 1053, 1361, 2112, 3138, 3223, 3240, 3336, 3462
PspPI GGNCC 6 cut(s) 916, 1052, 1360, 1475, 1788, 2933
PspPPI RGGWCCY 3 cut(s) 916, 1360, 1788
PstI CTGCAG 3 cut(s) 147, 710, 2620
PstNI CAGNNNCTG 1 cut(s) 2597
PsuI RGATCY 8 cut(s) 259, 385, 1299, 2110, 2735, 3136, 3318, 3460
PvuII CAGCTG 1 cut(s) 458
RseI CAYNNNNRTG 3 cut(s) 1413, 2898, 3357
SatI GCNGC 9 cut(s) 197, 245, 371, 980, 1382, 1567, 2586, 2843, 3415
Sau96I GGNCC 6 cut(s) 916, 1052, 1360, 1475, 1788, 2933
ScaI AGTACT 1 cut(s) 3015
SchI GAGTC 3 cut(s) 1754, 2603, 3413
ScrFI CCNGG 5 cut(s) 468, 873, 2218, 2490, 2937
SduI GDGCHC 1 cut(s) 1016
SfcI CTRYAG 6 cut(s) 32, 143, 706, 2565, 2616, 2955
SfuI TTCGAA 1 cut(s) 544
SinI GGWCC 4 cut(s) 916, 1360, 1475, 1788
SmiI ATTTAAAT 2 cut(s) 228, 354
SmiMI CAYNNNNRTG 3 cut(s) 1413, 2898, 3357
SmlI CTYRAG 2 cut(s) 2247, 2263
SmoI CTYRAG 2 cut(s) 2247, 2263
SpeI ACTAGT 2 cut(s) 5, 3199
SphI GCATGC 1 cut(s) 1090
SseBI AGGCCT 1 cut(s) 3397
SsiI CCGC 9 cut(s) 40, 569, 979, 1119, 2499, 2510, 2842, 3022, 3535
SspI AATATT 5 cut(s) 103, 726, 2306, 3071, 3088
SspMI CTAG 8 cut(s) 6, 510, 600, 732, 1017, 1091, 2996, 3200
StuI AGGCCT 1 cut(s) 3397
StyD4I CCNGG 5 cut(s) 466, 871, 2216, 2488, 2935
StyI CCWWGG 4 cut(s) 731, 1791, 2761, 3230
SwaI ATTTAAAT 2 cut(s) 228, 354
TaaI ACNGT 8 cut(s) 36, 531, 1061, 1335, 2239, 2755, 2956, 3339
TaiI ACGT 1 cut(s) 3508
TaqI TCGA 6 cut(s) 206, 544, 807, 1782, 2025, 2370
TatI WGTACW 5 cut(s) 109, 1750, 2121, 2186, 3013
TauI GCSGC 2 cut(s) 982, 2845
TfiI GAWTC 6 cut(s) 293, 419, 632, 2158, 2374, 2875
TscAI CASTG 6 cut(s) 1338, 1854, 2112, 2189, 2457, 2551
TseFI GTSAC 6 cut(s) 181, 1145, 2406, 2440, 2611, 3110
TseI GCWGC 7 cut(s) 196, 244, 370, 1381, 1566, 2585, 3414
Tsp45I GTSAC 6 cut(s) 181, 1145, 2406, 2440, 2611, 3110
TspGWI ACGGA 2 cut(s) 149, 790
TspRI CASTG 6 cut(s) 1338, 1854, 2112, 2189, 2457, 2551
Van91I CCANNNNNTGG 1 cut(s) 743
VpaK11BI GGWCC 4 cut(s) 916, 1360, 1475, 1788
XagI CCTNNNNNAGG 1 cut(s) 471
XceI RCATGY 7 cut(s) 1090, 1274, 1552, 1636, 1751, 2993, 3352
XmaJI CCTAGG 1 cut(s) 731
XmiI GTMKAC 1 cut(s) 2757
XmnI GAANNNNTTC 1 cut(s) 2391
XspI CTAG 8 cut(s) 6, 510, 600, 732, 1017, 1091, 2996, 3200
ZrmI AGTACT 1 cut(s) 3015
Zsp2I ATGCAT 2 cut(s) 1749, 3354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.