Rh4CG080600

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0012803
Physical Location & Seq
Forward (+)
14398616 .. 14404616
6001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG080600.1

Sequence Viewer

Length: 3423 bp
ATGACTAGTGAGGTTCGTGGTACTTCTGGACTACAGTTCCGCAAAGATGCAACAAATCATGTGCCAAGTAACACAAGTGGTGCAGATAGAGATGTAGATAATATTTGTGTACAGACGGGCGAGGAATTCTCAACGGAGTTTCTGCAGGATCGTATAGCACAAAGAAGATTAGCCCCAGTAGTGACTGGTGTTGAGCAGCACCAGTCGAAAAGAGTGGGATTTAATTTAAATCAAAACAATCCGCAGCTAGTTAATGAGGATCTAAATGGAATTCTTGGATTAAGGAGATTAGATTCTGATGGAAGTTCAGAATTTTCAGATTTTGTTACAGCTGGTACAGGTTATGTGCCTGATTTAGAGAAGAATGTTTTTCCTAGTAGTAATGTCAGCAGACAGTATGGTGGTCTTCGAAGACAACTATCAGGTAAGTTTGCGGATGAAGTTAATCGTGATTGGGTTATTCCTAGATCAAATACTCCACCTTTTTATGTTGGTGAATCCCCTCAGTCATATAGTCCATATGGTCAAGGATTTTTTGAAGGCTCTTTTTCTTTCAAAATGAAGTTTCTCTGCAGTTTTGGGGGGAGAATATTGCCTAGGCCAAATGATGGGAAGCTCAGATATGTTGGTGGAGATACACGGATTTTATCTATTCACAAAGGCACCAATTTCGAGGAACTAATTAAGAAGACATTGGCAATTTGTAACCACCCCCACACTATCAAGTACCAACTTCCTGGTGAGGAGCTTGATGCACTTGTATCTGTTTGTTCTGATGAGGACCTCCATCACATGATAGAGGAATATCATGAGCTGGAAAGAACTGAGGGTTCTCAAAGATTGCGGCTCTTTCTTGTACCTTTAAATGAACCAGAGTGCCCTAGTTCTATTGAAGGCAGGATCACACATCCAATGGGGCCTGACAGTCAATTCGTCTTTGCTGTCAATGGCATGCTAGACAGAAGTCCTCGGAAAAGCTCCAGCGGTCAGAGTTTGACAAGTCAGAATAGTCACTTTGGGAATGCCTCAGATTATAGTCCAACTTTTCATAGAGACACTTCCACATCTGCATTTACTTTAGAGAACAAGGATGTTAGTCTAAGTTCCTCAAATCTGTCGGGGACAAGTAGTAAACATGCTCGCCAGTTCTTAGGTACTCTTCAGATCCCACCGAAGTCTTTTATTCCATCCCCTCCACTGTCTCCTGTTCAACTTCAACATAGGGACCTCAAAAATTCTAATGTGCAGCTACATTTGGATCAACCATATTGTCATGGTAATGGAGGCATTGCTCTCTCTGATGTTGATGTTGTGGGCTATATCAATAATCTTGATCATGGTCCTCCATTGATGAATTACCATCAGCACAATAAAAATTTGGCTGAGACCTATCTGACTAAAAAATCCCACAACATGCCCTCCCTCAATCGCAGCGCAAGTGAAGATTTTGTGCCTTCTGCCCTGTATGGTCAAGGTGGCATGAACTTTGAGAGACACATGCCTCTTAGAAAGCCCTTGATCCATTCTGACAAGTCTGTCTCTCATCCTAAAGATATACTGGAGTTATTGTTACCGACTGGTAATATAGATGCCTCTCATCAAAAAATAATGCATGTACTTTCTGACTCTCAGTTGCACGAGCATCTCGAAAGGTCCTTGGAAGGAGAAAATGTCTCAGTACCATTGAATTTGATCAGAGAAGGATCACCTTCACTGGAGATGCCAAGTTCCTCGCAAGAACAAGAGGCTCAGCTGGTTGACCAGAAAAACAAAATGGTTACACATCAATTTAACATCAGCACACCTCACCATTGCAAGGAGGAAGAAGGTCTGAAGGTGTTCAACCATTACACGAATGACACTTCTGCTGATAAAAAGTGGAAGCACCTCGAAAGAAATGTTGAAGTTACTTCAAATGATGCTGTCATGGAATTTAAGAACTTACTAAATATGAACTACCTTAAAGATGCAGTGGACCCCCAAGAGTACAAATTAGACACAACTTCTCAGTATTCTCATAGGATTCAAAATGGTACAAATGATCAGCAGTGTACTACAACAATTATTGGTCAATCAATTTCCCGGCCGCATGGATTACTAACTGTTGCATCTCAAGAATTCTTAATCTCAAGCCCTCAAATGCCTACAAGTTCTGCTTCAGATAGAGAATATTCTCTGCATGATAACTATCCCGAGTATGTGGTTGAAAAGGTGGGTTCTGGCAGACACTCTTTCGAGAATCTGAAATGTGAAGATGTTCGCCCTGTCCAGTCACAGCCCTTAGATAATCATAATGACAATAAGGTGACAAAATCAGTGGTTATTGTTGAAGACTTAACTAATAGCACGCCACCTGGAATTCCCGCTTCTAAAGCGGTCTATCATGTCTCCCATATAGAAGATGAGACCAGTGATGAATGTTCATCACCTACAGAGACAGAGATAAGTGCAGCTCCAGAGTCTGAGAGAAAGGGTGTGACTGCAGATGGCAAAGATAGGCATGAAACTATTAGTGATGCTGTAATAGCCGAAATGGAAGCAGGCATCTATGGTTTGCAGATCATAAAAAATGCTGATCTTGAAGAACTGCAAGAGTTGGGTTCTGGTACATTTGGAACTGTCTACCATGGGAAGTGGAGAGGAACAGATGTTGCTATTAAGAGAATAAAAAAGAGTTGTTTTTCTGACAGATCATCAGAGCAAGAGCGGCTGACTAAGGACTTCTGGAGAGAGGCAAAGATTCTTTCCACCCTTCACCATCCAAATGTAGTAGCACTTTATGGAGTAGTACCTGATGGCCCTGGAGGAACATTGGCAACTGTAGCTGAATTTATGGTGAATGGATCATTGAGGCATGTCCTAGTTAGGAAGGACAGAGTACTTGACCGCCGAAAGAGGCTCATAATCTTGATGGACACAGCTTTTGGCATGGAATATTTGCATTTGAAAAATATTGTTCACTTTGATTTGAAGTGTGACAATTTGCTTGTCAATTTGAGGGATCCTGAAAGACCCATATGTAAGGTTGCTGATTTTGGGCTGTCAAGAATTAAACGCAACACACTAGTATCTGGTGGTGTGCGAGGAACCCTTCCATGGATGGCACCAGAATTATTAAATGGTAGCAGTAGCCGTGTCTCTGAGAAGGTTGATGTCTTCTCATTTGGTATTGTAATGTGGGAGATCTTGACTGGGGAGGAACCGTATGCCAACATGCATTGTGGTGCTATCATAGGGGGAATTGTAAATAATACACTAAGGCCTCCTATTCCAAAGCGCTGCGACTCGGAGTGGAAAAAGTTAATGGAAGAGTGCTGGTCGCCGGATCCTGCTGATCGGCCATCATTCACAGATATAACACATAGGCTACGTGATATGTCAACGGCACTCCAGAAGAAGCGGTCTAATGTTGCAAGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1140

Amino Acids

127.46

Weight (kDa)

6.05

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 202 - 287 1.2e-20 PB1 domain
Pkinase PF00069 861 - 1121 6.1e-50 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 862 - 1123 3.1e-63 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04700 AT1G04700 AT1G04700
fragaria_vesca FvH4_4g05890
malus_domestica MD13G1231300.v1.1 MD16G1235800.v1.1
prunus_persica Prupe.1G061200_v2.0.a1
pyrus_communis pycom13g20420
rosa_chinensis RchiOBHm_Chr4g0396991
rosa_laevigata RLG00000009461
rosa_multiflora Rmu_sc0002963.1_g000016
rosa_roxburghii Rroxscaffold_5G00341980
rosa_rugosa Rorug03G0346000
rosa_samantha Rh4AG075900 Rh4BG072300 Rh4CG080600 Rh4DG069700
rosa_wichuraiana Rw4G006090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1535
AccB1I GGYRCC 2 cut(s) 662, 3103
AccB7I CCANNNNNTGG 1 cut(s) 608
AccBSI CCGCTC 1 cut(s) 2707
AccI GTMKAC 1 cut(s) 2622
AcoI YGGCCR 2 cut(s) 2084, 3338
AcuI CTGAAG 3 cut(s) 1145, 1853, 2142
AfeI AGCGCT 1 cut(s) 3278
AfiI CCNNNNNNNGG 3 cut(s) 608, 1816, 3096
AhlI ACTAGT 2 cut(s) 5, 3064
AjnI CCWGG 3 cut(s) 736, 2353, 2800
AlwNI CAGNNNCTG 1 cut(s) 2462
Ama87I CYCGRG 1 cut(s) 2192
Aor51HI AGCGCT 1 cut(s) 3278
AoxI GGCC 6 cut(s) 599, 917, 2084, 2797, 3260, 3338
ApeKI GCWGC 6 cut(s) 196, 244, 1246, 1431, 2450, 3279
ArsI GACNNNNNNTTYG 4 cut(s) 914, 946, 2906, 2938
Asp700I GAANNNNTTC 2 cut(s) 1838, 2256
AspA2I CCTAGG 1 cut(s) 596
AspLEI GCGC 2 cut(s) 1436, 3279
AspS9I GGNCC 7 cut(s) 781, 917, 1225, 1340, 1653, 1975, 2798
AsuC2I CCSGG 1 cut(s) 2083
AsuHPI GGTGA 8 cut(s) 506, 752, 1698, 1799, 2317, 2418, 2747, 2848
AsuII TTCGAA 1 cut(s) 409
AvaI CYCGRG 1 cut(s) 2192
AvaII GGWCC 5 cut(s) 781, 1225, 1340, 1653, 1975
AvrII CCTAGG 1 cut(s) 596
BaeGI GKGCMC 1 cut(s) 881
BaeI ACNNNNGTAYC 2 cut(s) 3051, 3084
BamHI GGATCC 2 cut(s) 3001, 3325
BanI GGYRCC 2 cut(s) 662, 3103
BauI CACGAG 1 cut(s) 1637
BbsI GAAGAC 5 cut(s) 398, 418, 695, 2337, 3148
BbvI GCAGC 6 cut(s) 208, 256, 1258, 1443, 2462, 3266
BceAI ACGGC 2 cut(s) 3117, 3399
BcgI CGANNNNNNTGC 2 cut(s) 652, 686
BciT130I CCWGG 3 cut(s) 738, 2355, 2802
BclI TGATCA 3 cut(s) 1333, 1692, 2041
BcnI CCSGG 1 cut(s) 2083
BcuI ACTAGT 2 cut(s) 5, 3064
BfaI CTAG 9 cut(s) 6, 248, 375, 465, 597, 882, 956, 2861, 3065
BfmI CTRYAG 6 cut(s) 32, 143, 571, 2430, 2481, 2820
BfoI RGCGCY 1 cut(s) 3280
BglII AGATCT 1 cut(s) 3183
BisI GCNGC 9 cut(s) 197, 245, 845, 1247, 1432, 2087, 2451, 2708, 3280
BlnI CCTAGG 1 cut(s) 596
BlpI GCTNAGC 1 cut(s) 1749
BlsI GCNGC 9 cut(s) 198, 246, 846, 1248, 1433, 2088, 2452, 2709, 3281
BmcAI AGTACT 1 cut(s) 2880
Bme1390I CCNGG 4 cut(s) 738, 2083, 2355, 2802
Bme18I GGWCC 5 cut(s) 781, 1225, 1340, 1653, 1975
BmeT110I CYCGRG 1 cut(s) 2192
BmgT120I GGNCC 7 cut(s) 781, 917, 1225, 1340, 1653, 1975, 2798
BmiI GGNNCC 9 cut(s) 664, 918, 1226, 1977, 3003, 3088, 3105, 3201, 3327
BmrFI CCNGG 4 cut(s) 738, 2083, 2355, 2802
BmrI ACTGGG 2 cut(s) 170, 3201
BmuI ACTGGG 2 cut(s) 170, 3201
BoxI GACNNNNGTC 1 cut(s) 963
BpiI GAAGAC 5 cut(s) 398, 418, 695, 2337, 3148
BplI GAGNNNNNCTC 2 cut(s) 113, 145
BpmI CTGGAG 7 cut(s) 964, 1580, 1736, 2439, 2746, 2823, 3374
Bpu1102I GCTNAGC 1 cut(s) 1749
Bpu14I TTCGAA 1 cut(s) 409
BpuEI CTTGAG 2 cut(s) 2097, 2113
BpuMI CCSGG 1 cut(s) 2083
BsaAI YACGTR 1 cut(s) 3371
BsaBI GATNNNNATC 1 cut(s) 2187
BsaI GGTCTC 2 cut(s) 1379, 2399
BsaJI CCNNGG 6 cut(s) 596, 968, 1656, 2626, 2800, 3095
BsaXI ACNNNNNCTCC 8 cut(s) 1403, 1433, 1553, 1583, 1656, 1686, 3188, 3218
Bsc4I CCNNNNNNNGG 3 cut(s) 608, 1816, 3096
Bse3DI GCAATG 2 cut(s) 1287, 1810
Bse8I GATNNNNATC 1 cut(s) 2187
BseBI CCWGG 3 cut(s) 738, 2355, 2802
BseDI CCNNGG 6 cut(s) 596, 968, 1656, 2626, 2800, 3095
BseGI GGATG 7 cut(s) 442, 907, 1096, 1187, 1543, 2758, 3105
BseJI GATNNNNATC 1 cut(s) 2187
BseLI CCNNNNNNNGG 3 cut(s) 608, 1816, 3096
BseMI GCAATG 2 cut(s) 1287, 1810
BseRI GAGGAG 1 cut(s) 758
BseSI GKGCMC 1 cut(s) 881
BseX3I CGGCCG 1 cut(s) 2084
BseXI GCAGC 6 cut(s) 208, 256, 1258, 1443, 2462, 3266
BsgI GTGCAG 3 cut(s) 102, 1265, 2469
Bsh1285I CGRYCG 1 cut(s) 2087
BshFI GGCC 6 cut(s) 601, 919, 2086, 2799, 3262, 3340
BshNI GGYRCC 2 cut(s) 662, 3103
BsiEI CGRYCG 1 cut(s) 2087
BsiHKCI CYCGRG 1 cut(s) 2192
BsiSI CCGG 2 cut(s) 2083, 3323
BslFI GGGAC 2 cut(s) 1135, 1238
BslI CCNNNNNNNGG 3 cut(s) 608, 1816, 3096
BsmFI GGGAC 2 cut(s) 1135, 1238
BsmI GAATGC 1 cut(s) 1027
BsnI GGCC 6 cut(s) 601, 919, 2086, 2799, 3262, 3340
Bso31I GGTCTC 2 cut(s) 1379, 2399
BsoBI CYCGRG 1 cut(s) 2192
Bsp119I TTCGAA 1 cut(s) 409
Bsp1286I GDGCHC 1 cut(s) 881
Bsp1407I TGTACA 1 cut(s) 109
Bsp1720I GCTNAGC 1 cut(s) 1749
Bsp19I CCATGG 2 cut(s) 2626, 3095
BspANI GGCC 6 cut(s) 601, 919, 2086, 2799, 3262, 3340
BspHI TCATGA 1 cut(s) 808
BspLI GGNNCC 9 cut(s) 664, 918, 1226, 1977, 3003, 3088, 3105, 3201, 3327
BspMAI CTGCAG 3 cut(s) 147, 575, 2485
BspT104I TTCGAA 1 cut(s) 409
BspT107I GGYRCC 2 cut(s) 662, 3103
BspTNI GGTCTC 2 cut(s) 1379, 2399
BsrBI CCGCTC 1 cut(s) 2707
BsrDI GCAATG 2 cut(s) 1287, 1810
BsrGI TGTACA 1 cut(s) 109
BssECI CCNNGG 6 cut(s) 596, 968, 1656, 2626, 2800, 3095
BssSI CACGAG 1 cut(s) 1637
BssT1I CCWWGG 4 cut(s) 596, 1656, 2626, 3095
Bst2BI CACGAG 1 cut(s) 1637
Bst2UI CCWGG 3 cut(s) 738, 2355, 2802
Bst4CI ACNGT 8 cut(s) 36, 396, 926, 1200, 2104, 2620, 2821, 3204
Bst6I CTCTTC 2 cut(s) 1164, 3303
BstAUI TGTACA 1 cut(s) 109
BstBAI YACGTR 1 cut(s) 3371
BstBI TTCGAA 1 cut(s) 409
BstC8I GCNNGC 4 cut(s) 953, 1141, 2348, 2542
BstDSI CCRYGG 2 cut(s) 2626, 3095
BstF5I GGATG 7 cut(s) 442, 907, 1096, 1187, 1543, 2758, 3105
BstH2I RGCGCY 1 cut(s) 3280
BstHHI GCGC 2 cut(s) 1436, 3279
BstMCI CGRYCG 1 cut(s) 2087
BstMWI GCNNNNNNNGC 4 cut(s) 2372, 2525, 2707, 2822
BstNI CCWGG 3 cut(s) 738, 2355, 2802
BstNSI RCATGY 7 cut(s) 955, 1139, 1417, 1501, 1616, 2858, 3217
BstPAI GACNNNNGTC 1 cut(s) 963
BstSCI CCNGG 4 cut(s) 736, 2081, 2353, 2800
BstSFI CTRYAG 6 cut(s) 32, 143, 571, 2430, 2481, 2820
BstSLI GKGCMC 1 cut(s) 881
BstV1I GCAGC 6 cut(s) 208, 256, 1258, 1443, 2462, 3266
BstV2I GAAGAC 5 cut(s) 398, 418, 695, 2337, 3148
BstX2I RGATCY 5 cut(s) 259, 1164, 3001, 3183, 3325
BstXI CCANNNNNNTGG 1 cut(s) 737
BstYI RGATCY 5 cut(s) 259, 1164, 3001, 3183, 3325
BstZI CGGCCG 1 cut(s) 2084
BsuRI GGCC 6 cut(s) 601, 919, 2086, 2799, 3262, 3340
BtgI CCRYGG 2 cut(s) 2626, 3095
BtsCI GGATG 7 cut(s) 442, 907, 1096, 1187, 1543, 2758, 3105
BtsI GCAGTG 2 cut(s) 1977, 2054
BtsIMutI CAGTG 6 cut(s) 1196, 1712, 1977, 2054, 2322, 2416
Cac8I GCNNGC 4 cut(s) 953, 1141, 2348, 2542
CaiI CAGNNNCTG 1 cut(s) 2462
CciI TCATGA 1 cut(s) 808
CfoI GCGC 2 cut(s) 1436, 3279
Cfr13I GGNCC 7 cut(s) 781, 917, 1225, 1340, 1653, 1975, 2798
CspCI CAANNNNNGTGG 2 cut(s) 2298, 2333
DraI TTTAAA 2 cut(s) 228, 864
DrdI GACNNNNNNGTC 1 cut(s) 1535
DseDI GACNNNNNNGTC 1 cut(s) 1535
EaeI YGGCCR 2 cut(s) 2084, 3338
EagI CGGCCG 1 cut(s) 2084
Eam1104I CTCTTC 2 cut(s) 1164, 3303
EarI CTCTTC 2 cut(s) 1164, 3303
EclXI CGGCCG 1 cut(s) 2084
Eco130I CCWWGG 4 cut(s) 596, 1656, 2626, 3095
Eco147I AGGCCT 1 cut(s) 3262
Eco31I GGTCTC 2 cut(s) 1379, 2399
Eco47I GGWCC 5 cut(s) 781, 1225, 1340, 1653, 1975
Eco47III AGCGCT 1 cut(s) 3278
Eco52I CGGCCG 1 cut(s) 2084
Eco57I CTGAAG 3 cut(s) 1145, 1853, 2142
Eco88I CYCGRG 1 cut(s) 2192
EcoO109I RGGNCCY 4 cut(s) 781, 917, 1225, 1653
EcoRI GAATTC 4 cut(s) 125, 270, 2117, 2358
EcoRII CCWGG 3 cut(s) 736, 2353, 2800
EcoT14I CCWWGG 4 cut(s) 596, 1656, 2626, 3095
EcoT22I ATGCAT 2 cut(s) 1614, 3219
ErhI CCWWGG 4 cut(s) 596, 1656, 2626, 3095
FalI AAGNNNNNCTT 2 cut(s) 2140, 2172
FaqI GGGAC 2 cut(s) 1135, 1238
FauI CCCGC 1 cut(s) 2371
FauNDI CATATG 2 cut(s) 520, 3017
FbaI TGATCA 3 cut(s) 1333, 1692, 2041
FblI GTMKAC 1 cut(s) 2622
Fnu4HI GCNGC 9 cut(s) 197, 245, 845, 1247, 1432, 2087, 2451, 2708, 3280
FokI GGATG 7 cut(s) 449, 894, 1103, 1174, 1530, 2745, 3112
Fsp4HI GCNGC 9 cut(s) 197, 245, 845, 1247, 1432, 2087, 2451, 2708, 3280
FspBI CTAG 9 cut(s) 6, 248, 375, 465, 597, 882, 956, 2861, 3065
GlaI GCGC 2 cut(s) 1435, 3278
GluI GCNGC 9 cut(s) 197, 245, 845, 1247, 1432, 2087, 2451, 2708, 3280
GsuI CTGGAG 7 cut(s) 964, 1580, 1736, 2439, 2746, 2823, 3374
HaeII RGCGCY 1 cut(s) 3280
HaeIII GGCC 6 cut(s) 601, 919, 2086, 2799, 3262, 3340
HapII CCGG 2 cut(s) 2083, 3323
HhaI GCGC 2 cut(s) 1436, 3279
Hin6I GCGC 2 cut(s) 1434, 3277
HinP1I GCGC 2 cut(s) 1434, 3277
HincII GTYRAC 2 cut(s) 1759, 3381
HindII GTYRAC 2 cut(s) 1759, 3381
HinfI GANTC 8 cut(s) 293, 497, 1625, 2023, 2239, 2459, 2740, 3284
HpaII CCGG 2 cut(s) 2083, 3323
HphI GGTGA 8 cut(s) 506, 752, 1698, 1799, 2317, 2418, 2747, 2848
Hpy166II GTNNAC 8 cut(s) 110, 1133, 1759, 1975, 2052, 2623, 2959, 3381
Hpy8I GTNNAC 8 cut(s) 110, 1133, 1759, 1975, 2052, 2623, 2959, 3381
HpyCH4III ACNGT 8 cut(s) 36, 396, 926, 1200, 2104, 2620, 2821, 3204
HpyCH4IV ACGT 1 cut(s) 3370
HpyF10VI GCNNNNNNNGC 4 cut(s) 2372, 2525, 2707, 2822
HpySE526I ACGT 1 cut(s) 3370
HspAI GCGC 2 cut(s) 1434, 3277
Ksp22I TGATCA 3 cut(s) 1333, 1692, 2041
LmnI GCTCC 3 cut(s) 745, 983, 2458
Lsp1109I GCAGC 6 cut(s) 208, 256, 1258, 1443, 2462, 3266
MaeI CTAG 9 cut(s) 6, 248, 375, 465, 597, 882, 956, 2861, 3065
MaeII ACGT 1 cut(s) 3370
MbiI CCGCTC 1 cut(s) 2707
MflI RGATCY 5 cut(s) 259, 1164, 3001, 3183, 3325
MhlI GDGCHC 1 cut(s) 881
MlyI GAGTC 3 cut(s) 1619, 2468, 3278
MmeI TCCRAC 1 cut(s) 1064
Mph1103I ATGCAT 2 cut(s) 1614, 3219
MroXI GAANNNNTTC 2 cut(s) 1838, 2256
MslI CAYNNNNRTG 3 cut(s) 1278, 2763, 3222
MspA1I CMGCKG 3 cut(s) 332, 984, 1753
MspI CCGG 2 cut(s) 2083, 3323
MspR9I CCNGG 4 cut(s) 738, 2083, 2355, 2802
Mva1269I GAATGC 1 cut(s) 1027
MvaI CCWGG 3 cut(s) 738, 2355, 2802
MwoI GCNNNNNNNGC 4 cut(s) 2372, 2525, 2707, 2822
NciI CCSGG 1 cut(s) 2083
NcoI CCATGG 2 cut(s) 2626, 3095
NdeI CATATG 2 cut(s) 520, 3017
NlaIV GGNNCC 9 cut(s) 664, 918, 1226, 1977, 3003, 3088, 3105, 3201, 3327
NmuCI GTSAC 6 cut(s) 181, 1010, 2271, 2305, 2476, 2975
NsiI ATGCAT 2 cut(s) 1614, 3219
NspI RCATGY 7 cut(s) 955, 1139, 1417, 1501, 1616, 2858, 3217
NspV TTCGAA 1 cut(s) 409
PaeI GCATGC 1 cut(s) 955
PagI TCATGA 1 cut(s) 808
PceI AGGCCT 1 cut(s) 3262
PcsI WCGNNNNNNNCGW 1 cut(s) 1644
PctI GAATGC 1 cut(s) 1027
PdmI GAANNNNTTC 2 cut(s) 1838, 2256
PfeI GAWTC 5 cut(s) 293, 497, 2023, 2239, 2740
PflMI CCANNNNNTGG 1 cut(s) 608
PkrI GCNGC 9 cut(s) 198, 246, 846, 1248, 1433, 2088, 2452, 2709, 3281
PleI GAGTC 3 cut(s) 1619, 2467, 3278
PpsI GAGTC 3 cut(s) 1619, 2467, 3278
Ppu21I YACGTR 1 cut(s) 3371
PpuMI RGGWCCY 3 cut(s) 781, 1225, 1653
PshAI GACNNNNGTC 1 cut(s) 963
Psp5II RGGWCCY 3 cut(s) 781, 1225, 1653
Psp6I CCWGG 3 cut(s) 736, 2353, 2800
PspGI CCWGG 3 cut(s) 736, 2353, 2800
PspN4I GGNNCC 9 cut(s) 664, 918, 1226, 1977, 3003, 3088, 3105, 3201, 3327
PspPI GGNCC 7 cut(s) 781, 917, 1225, 1340, 1653, 1975, 2798
PspPPI RGGWCCY 3 cut(s) 781, 1225, 1653
PstI CTGCAG 3 cut(s) 147, 575, 2485
PstNI CAGNNNCTG 1 cut(s) 2462
PsuI RGATCY 5 cut(s) 259, 1164, 3001, 3183, 3325
PvuII CAGCTG 2 cut(s) 332, 1753
RseI CAYNNNNRTG 3 cut(s) 1278, 2763, 3222
SatI GCNGC 9 cut(s) 197, 245, 845, 1247, 1432, 2087, 2451, 2708, 3280
Sau96I GGNCC 7 cut(s) 781, 917, 1225, 1340, 1653, 1975, 2798
ScaI AGTACT 1 cut(s) 2880
SchI GAGTC 3 cut(s) 1619, 2468, 3278
ScrFI CCNGG 4 cut(s) 738, 2083, 2355, 2802
SduI GDGCHC 1 cut(s) 881
SfcI CTRYAG 6 cut(s) 32, 143, 571, 2430, 2481, 2820
SfuI TTCGAA 1 cut(s) 409
SinI GGWCC 5 cut(s) 781, 1225, 1340, 1653, 1975
SmiI ATTTAAAT 1 cut(s) 228
SmiMI CAYNNNNRTG 3 cut(s) 1278, 2763, 3222
SmlI CTYRAG 2 cut(s) 2112, 2128
SmoI CTYRAG 2 cut(s) 2112, 2128
SpeI ACTAGT 2 cut(s) 5, 3064
SphI GCATGC 1 cut(s) 955
SseBI AGGCCT 1 cut(s) 3262
SspI AATATT 5 cut(s) 103, 591, 2171, 2936, 2953
SspMI CTAG 9 cut(s) 6, 248, 375, 465, 597, 882, 956, 2861, 3065
StuI AGGCCT 1 cut(s) 3262
StyD4I CCNGG 4 cut(s) 736, 2081, 2353, 2800
StyI CCWWGG 4 cut(s) 596, 1656, 2626, 3095
SwaI ATTTAAAT 1 cut(s) 228
TaaI ACNGT 8 cut(s) 36, 396, 926, 1200, 2104, 2620, 2821, 3204
TaiI ACGT 1 cut(s) 3373
TaqI TCGA 6 cut(s) 206, 409, 672, 1647, 1890, 2235
TatI WGTACW 5 cut(s) 109, 1615, 1986, 2051, 2878
TauI GCSGC 3 cut(s) 847, 2089, 2710
TfiI GAWTC 5 cut(s) 293, 497, 2023, 2239, 2740
TscAI CASTG 6 cut(s) 1203, 1719, 1977, 2054, 2322, 2416
TseFI GTSAC 6 cut(s) 181, 1010, 2271, 2305, 2476, 2975
TseI GCWGC 6 cut(s) 196, 244, 1246, 1431, 2450, 3279
Tsp45I GTSAC 6 cut(s) 181, 1010, 2271, 2305, 2476, 2975
TspGWI ACGGA 2 cut(s) 149, 655
TspRI CASTG 6 cut(s) 1203, 1719, 1977, 2054, 2322, 2416
Van91I CCANNNNNTGG 1 cut(s) 608
VpaK11BI GGWCC 5 cut(s) 781, 1225, 1340, 1653, 1975
XceI RCATGY 7 cut(s) 955, 1139, 1417, 1501, 1616, 2858, 3217
XmaJI CCTAGG 1 cut(s) 596
XmiI GTMKAC 1 cut(s) 2622
XmnI GAANNNNTTC 2 cut(s) 1838, 2256
XspI CTAG 9 cut(s) 6, 248, 375, 465, 597, 882, 956, 2861, 3065
ZrmI AGTACT 1 cut(s) 2880
Zsp2I ATGCAT 2 cut(s) 1614, 3219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.