Rh4BG072300

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0012803
Physical Location & Seq
Forward (+)
12719615 .. 12726261
6647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG072300.1

Sequence Viewer

Length: 3045 bp
ATGACTAGTGAGGTTCGTGGTACTTCTGGACAACAGTTCCGCAAAGATGCAACAAATCATGTGCCAAGTGACACAAGTGGTGCAGATAGAGATGTAGATAATATTTGTGTACAGACGGGCGAGGAATTCTCAACGGAGTTTCTGCAGGATCGTATAGCACAAAGAAGATTAGCCCCAGTAGTGACTGGTGTTGAGCAGCACCAGTCAAAAAGAGTGGGATTTAATTTAAATCAAAACAATCTGCAGCTCGTTAATGAGGATCTAAATGGAATTCTTGGATTAAGGAGATTAGATTCTGATGGAAGTTCAGAATTTTCAGATTTTGTTACAGCTGGTACAGGTTATGTGCCTGATTTAGAGAAGAATGTTTTTCCTAGTAGTAATGTTAGCAGACAGTATGGTGGTCTTCGAAGACAACTATCAGGTAAGTTTGCGGATGAAGTTAATCGTGATTGGATTATTCCTAGATCAAATACTCCACCTTTTTATGTTGGTGAATCCCCTCAGTCATATAGTCCATATGGTCAAGGATTTTTTGAAGGCTCTTTCTCTTTCAAAATGAAGTTTCTCTGCAGTTTTGGGGGGAGAATATTGCCTAGGCCAAATGATGGGAAGCTCAGATATGTTGGTGGAGATACACGGATTTTATCTATTCACAAAAGCACCAATTTCGAGGAACTAATGAAGAAGACATTGGCAATTTGTAACCACCCCCACACTATCAAGTACCAACTTCCTGGTGAGGAGCTTGATGCACTTGTATCTGTTTGTTCTGATGAGGACCTCCATCACATGATAGAGGAATATCATGAGCTGGAAAGAACTGAGGGTTCTCAAAGATTGCGGCTCTTTCTTGTACCTTTAAATGAACCAGAGAGCCCTAGTTCTATTGAAGGCAGGGTCACACATCCAATGGGGCCTGACAGTCAATTCGTCTTTGCTGTCAATGGCATGCTAGACAGAAGTCCTCGGAAAAGCTCCAGCGGTCAGAGTTTGACAAGTCAGAATAGTCACTTTGGGAATGCCTCAGATTATAGTCCAACTTTTCATAGAGACACTTCCACATCTGCATTTACTTTAGAGAACAAGGATGTTAGTCTAAGTTCCTCAAATCTGTCGGGGACAAGTAGTAAACATGCTCGCCAGTTCTTAGGTACTCTTCAGATCCCACCGAAGTCTTTTATTCCATCCCCTCCACTGTCTCCTGTTCAACTTCAACATAGGGACCTCAAAAATTCTAATGTGCAGCTACGTTTGGATCAACCATATTGTCATGGTAATGGAGGCATTGCTCTCTCTGATGTTGATGCTGTGGGCTATATCAATAATCTTGATCATGGTTCTCCATTGATGAATTACCATCAGCACAATAAAAATTTGACTGAGACCTACCTGACTAAAAAATCCCACAACATGCCCTCCCTCAATCGCAGCGCAAGTGAAGATTTTGTGCCTTCTGCCCAGTATGGTCAAGGTGGCATGAACTTTGAGAGACACATGCCTCTTAGAAAGCCCTTGATCCATTCTGACATGTCTGTCTCTCATCCTAAAGATATGCTGGAGTTATTGTTACCGACTGGTAATATAGATGCCTCTCATCAAAAAATAATGCATGTACTTTCTGACTCTCGGTTGCACGAGCATCTCGAAAGGTCCTTGGAAGGAGAAAATGTCTCAGTGCCATTGAATTTGATCAGAGAAGGATCACCTTCACTGGAGATGCCAAGTTCCTCGCAAGAACGAGAGGCTCAGCTGGTTGACCAGAAAAACAAAATGGTTACACATAGTAATCAATTTAATATCAGCACACCTCACCATTGCAAGGAGGAAGAAGGTCTGGAGGTGTTCAACCATTACACGAATGACACTTCTGCTGATAAAAAATGGAAGCACTTCGAAGGAAATGTTGAAGTTACTTCAAATGATGCTGTCATGGAATTTAAGAACTTTCTAAATATGAACTACCTTAAAGATGCAGTGGACCCCCAAGAGTACAAATTAGACACAACTTCTCAGTATTCTCATAGGATTCAAAATGGTACAAATGATCAGCAGTGTACTACAACAATTAGTGGTCAAACAATTTCCCAGCCCCATGGATTACTAACTGTTGCATCTCAAGAATTCTTAATCTCAAGCCCTCAAATGCCTACAAGTTCTGCTTCAGATAGAGAATATTCTCTGCATGATAATTATCCCGAGTATGTGGTTGAAAAGGTGGGTTCTGGCAGACACTCTTTCGAGAATCTGAAATGTGAAGATGTTCGCCCTGTCCAGTCACAGCCCTTAGATAATCATAATGACAATAAGGTGACAAAATCAGTGGTTATTGTTGAAGACTTAACTAATAGCACACCACCTGGAATTCCCGCTTCTAAAGCGGTCTATCGTGTCTCCCATATAGAAGATGAGACCAGTGATGAATGTTCATCACCTACAGAGACAGAGATAAGTGCAGCTCCAGAGTCTGAGGGAAAGGGTGTGACTGCAGATGGCAAAGATAGGCATGAAACTATTAGTGATGCTGCAATAGCCGAAATGGAAGCAGGCATCTATGGTTTGCAGATCATAAAAAATGCTGATCTTGAAGAACTGCAAGAGTTGGGATCTGGTACATTTGGAACTGTCTACCATGGGAAGTGGAGAGGAACAGATGTTGCTATTAAGAGAATAAAAAAGAGTTGTTTTTCTGACAGATCATCAGAGCAAGAGCGGCTGACTAAGGACTTCTGGAGAGAGGCAAAGATTCTTTCCACCCTTCACCATCCAAATGTAGTAGCACTTTATGGAGTAGTACCTGATGGCCCTGGAGGAACATTGGCAACTGTAGCTGAATTTATGGTGAATGGATCATTGAGGCATGTCCTAGTTAGGAAGGACAGGCCTCCTATTCCAAAGCGCTGCGACTCGGAGTGGAAAAAGTTAATGGAAGAGTGCTGGTCGCCGGATCCTGCTGATCGGCCATCATTCACAGATATAACACATAGGCTACGTGATATGTCAACGGCACTCCAGAAGAAGCGGTCTAATGTTGCAAGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1014

Amino Acids

113.27

Weight (kDa)

5.81

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 202 - 287 4.1e-21 PB1 domain
Pkinase PF00069 863 - 959 1.1e-12 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 864 - 960 5.3e-16 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 957 - 997 2.4e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04700 AT1G04700 AT1G04700
fragaria_vesca FvH4_4g05890
malus_domestica MD13G1231300.v1.1 MD16G1235800.v1.1
prunus_persica Prupe.1G061200_v2.0.a1
pyrus_communis pycom13g20420
rosa_chinensis RchiOBHm_Chr4g0396991
rosa_laevigata RLG00000009461
rosa_multiflora Rmu_sc0002963.1_g000016
rosa_roxburghii Rroxscaffold_5G00341980
rosa_rugosa Rorug03G0346000
rosa_samantha Rh4AG075900 Rh4BG072300 Rh4CG080600 Rh4DG069700
rosa_wichuraiana Rw4G006090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1535
AccB7I CCANNNNNTGG 1 cut(s) 608
AccBSI CCGCTC 1 cut(s) 2713
AccI GTMKAC 1 cut(s) 2628
AciI CCGC 8 cut(s) 40, 434, 844, 984, 2370, 2381, 2713, 3022
AcoI YGGCCR 1 cut(s) 2960
AcuI CTGAAG 2 cut(s) 1145, 2148
AfeI AGCGCT 1 cut(s) 2900
AfiI CCNNNNNNNGG 2 cut(s) 608, 1822
AflIII ACRYGT 1 cut(s) 1530
AhlI ACTAGT 1 cut(s) 5
AjnI CCWGG 3 cut(s) 736, 2359, 2806
AjuI GAANNNNNNNTTGG 2 cut(s) 677, 709
Alw26I GTCTC 9 cut(s) 1047, 1206, 1379, 1486, 1543, 1678, 2398, 2405, 2435
AlwNI CAGNNNCTG 1 cut(s) 2468
Ama87I CYCGRG 1 cut(s) 2198
Aor51HI AGCGCT 1 cut(s) 2900
AoxI GGCC 5 cut(s) 599, 917, 2803, 2882, 2960
ApeKI GCWGC 7 cut(s) 196, 244, 1246, 1431, 2456, 2525, 2901
ArsI GACNNNNNNTTYG 2 cut(s) 914, 946
Asp700I GAANNNNTTC 2 cut(s) 1892, 2262
AspA2I CCTAGG 1 cut(s) 596
AspLEI GCGC 2 cut(s) 1436, 2901
AspS9I GGNCC 6 cut(s) 781, 917, 1225, 1653, 1981, 2804
AsuHPI GGTGA 8 cut(s) 506, 752, 1698, 1805, 2323, 2424, 2753, 2854
AsuII TTCGAA 2 cut(s) 409, 1896
AvaI CYCGRG 1 cut(s) 2198
AvaII GGWCC 4 cut(s) 781, 1225, 1653, 1981
AvrII CCTAGG 1 cut(s) 596
BamHI GGATCC 1 cut(s) 2947
BanII GRGCYC 1 cut(s) 881
BauI CACGAG 1 cut(s) 1637
BbsI GAAGAC 4 cut(s) 398, 418, 695, 2343
BbvI GCAGC 7 cut(s) 208, 256, 1258, 1443, 2468, 2512, 2888
BccI CCATC 9 cut(s) 293, 602, 795, 1195, 1368, 2486, 2772, 2795, 2971
BceAI ACGGC 1 cut(s) 3021
BcgI CGANNNNNNTGC 2 cut(s) 652, 686
BciT130I CCWGG 3 cut(s) 738, 2361, 2808
BclI TGATCA 3 cut(s) 1333, 1692, 2047
BcoDI GTCTC 9 cut(s) 1047, 1206, 1379, 1486, 1543, 1678, 2398, 2405, 2435
BcuI ACTAGT 1 cut(s) 5
BfaI CTAG 7 cut(s) 6, 375, 465, 597, 882, 956, 2867
BfmI CTRYAG 6 cut(s) 143, 242, 571, 2436, 2487, 2826
BfoI RGCGCY 1 cut(s) 2902
BisI GCNGC 9 cut(s) 197, 245, 845, 1247, 1432, 2457, 2526, 2714, 2902
BlnI CCTAGG 1 cut(s) 596
BlpI GCTNAGC 1 cut(s) 1749
BlsI GCNGC 9 cut(s) 198, 246, 846, 1248, 1433, 2458, 2527, 2715, 2903
Bme1390I CCNGG 3 cut(s) 738, 2361, 2808
Bme18I GGWCC 4 cut(s) 781, 1225, 1653, 1981
BmeT110I CYCGRG 1 cut(s) 2198
BmgT120I GGNCC 6 cut(s) 781, 917, 1225, 1653, 1981, 2804
BmiI GGNNCC 4 cut(s) 918, 1226, 1983, 2949
BmrFI CCNGG 3 cut(s) 738, 2361, 2808
BmrI ACTGGG 2 cut(s) 170, 1456
BmuI ACTGGG 2 cut(s) 170, 1456
BoxI GACNNNNGTC 1 cut(s) 963
BpiI GAAGAC 4 cut(s) 398, 418, 695, 2343
BplI GAGNNNNNCTC 2 cut(s) 113, 145
BpmI CTGGAG 8 cut(s) 964, 1580, 1736, 1859, 2445, 2752, 2829, 2996
Bpu1102I GCTNAGC 1 cut(s) 1749
Bpu14I TTCGAA 2 cut(s) 409, 1896
BpuEI CTTGAG 2 cut(s) 2103, 2119
BsaAI YACGTR 1 cut(s) 2993
BsaBI GATNNNNATC 1 cut(s) 2193
BsaI GGTCTC 2 cut(s) 1379, 2405
BsaJI CCNNGG 6 cut(s) 596, 968, 1656, 2095, 2632, 2806
BsaXI ACNNNNNCTCC 6 cut(s) 1403, 1433, 1553, 1583, 1656, 1686
Bsc4I CCNNNNNNNGG 2 cut(s) 608, 1822
Bse1I ACTGG 9 cut(s) 176, 190, 202, 1144, 1462, 1582, 1719, 2275, 2415
Bse3DI GCAATG 2 cut(s) 1287, 1816
Bse8I GATNNNNATC 1 cut(s) 2193
BseBI CCWGG 3 cut(s) 738, 2361, 2808
BseDI CCNNGG 6 cut(s) 596, 968, 1656, 2095, 2632, 2806
BseGI GGATG 6 cut(s) 442, 907, 1096, 1187, 1543, 2764
BseJI GATNNNNATC 1 cut(s) 2193
BseLI CCNNNNNNNGG 2 cut(s) 608, 1822
BseMI GCAATG 2 cut(s) 1287, 1816
BseMII CTCAG 9 cut(s) 518, 631, 816, 1041, 1374, 1689, 1763, 2027, 2460
BseNI ACTGG 9 cut(s) 176, 190, 202, 1144, 1462, 1582, 1719, 2275, 2415
BseRI GAGGAG 1 cut(s) 758
BseXI GCAGC 7 cut(s) 208, 256, 1258, 1443, 2468, 2512, 2888
BseYI CCCAGC 1 cut(s) 2088
BsgI GTGCAG 3 cut(s) 102, 1265, 2475
BshFI GGCC 5 cut(s) 601, 919, 2805, 2884, 2962
BsiHKCI CYCGRG 1 cut(s) 2198
BsiSI CCGG 1 cut(s) 2945
BslFI GGGAC 2 cut(s) 1135, 1238
BslI CCNNNNNNNGG 2 cut(s) 608, 1822
BsmAI GTCTC 9 cut(s) 1047, 1206, 1379, 1486, 1543, 1678, 2398, 2405, 2435
BsmFI GGGAC 2 cut(s) 1135, 1238
BsmI GAATGC 1 cut(s) 1027
BsnI GGCC 5 cut(s) 601, 919, 2805, 2884, 2962
Bso31I GGTCTC 2 cut(s) 1379, 2405
BsoBI CYCGRG 1 cut(s) 2198
Bsp119I TTCGAA 2 cut(s) 409, 1896
Bsp1286I GDGCHC 1 cut(s) 881
Bsp1407I TGTACA 1 cut(s) 109
Bsp1720I GCTNAGC 1 cut(s) 1749
Bsp19I CCATGG 2 cut(s) 2095, 2632
BspACI CCGC 8 cut(s) 40, 434, 844, 984, 2370, 2381, 2713, 3022
BspANI GGCC 5 cut(s) 601, 919, 2805, 2884, 2962
BspCNI CTCAG 9 cut(s) 517, 630, 817, 1040, 1375, 1688, 1762, 2026, 2461
BspHI TCATGA 1 cut(s) 808
BspLI GGNNCC 4 cut(s) 918, 1226, 1983, 2949
BspMAI CTGCAG 4 cut(s) 147, 246, 575, 2491
BspT104I TTCGAA 2 cut(s) 409, 1896
BspTNI GGTCTC 2 cut(s) 1379, 2405
BsrBI CCGCTC 1 cut(s) 2713
BsrDI GCAATG 2 cut(s) 1287, 1816
BsrGI TGTACA 1 cut(s) 109
BsrI ACTGG 9 cut(s) 176, 190, 202, 1144, 1462, 1582, 1719, 2275, 2415
BssECI CCNNGG 6 cut(s) 596, 968, 1656, 2095, 2632, 2806
BssSI CACGAG 1 cut(s) 1637
BssT1I CCWWGG 4 cut(s) 596, 1656, 2095, 2632
Bst2BI CACGAG 1 cut(s) 1637
Bst2UI CCWGG 3 cut(s) 738, 2361, 2808
Bst4CI ACNGT 7 cut(s) 36, 396, 926, 1200, 2110, 2626, 2827
Bst6I CTCTTC 2 cut(s) 1164, 2925
BstAUI TGTACA 1 cut(s) 109
BstBAI YACGTR 1 cut(s) 2993
BstBI TTCGAA 2 cut(s) 409, 1896
BstC8I GCNNGC 3 cut(s) 953, 1141, 2548
BstDSI CCRYGG 2 cut(s) 2095, 2632
BstF5I GGATG 6 cut(s) 442, 907, 1096, 1187, 1543, 2764
BstH2I RGCGCY 1 cut(s) 2902
BstHHI GCGC 2 cut(s) 1436, 2901
BstMAI GTCTC 9 cut(s) 1047, 1206, 1379, 1486, 1543, 1678, 2398, 2405, 2435
BstMWI GCNNNNNNNGC 4 cut(s) 2378, 2531, 2713, 2828
BstNI CCWGG 3 cut(s) 738, 2361, 2808
BstNSI RCATGY 7 cut(s) 955, 1139, 1417, 1501, 1534, 1616, 2864
BstPAI GACNNNNGTC 1 cut(s) 963
BstSCI CCNGG 3 cut(s) 736, 2359, 2806
BstSFI CTRYAG 6 cut(s) 143, 242, 571, 2436, 2487, 2826
BstV1I GCAGC 7 cut(s) 208, 256, 1258, 1443, 2468, 2512, 2888
BstV2I GAAGAC 4 cut(s) 398, 418, 695, 2343
BstX2I RGATCY 4 cut(s) 259, 1164, 2606, 2947
BstXI CCANNNNNNTGG 2 cut(s) 737, 2096
BstYI RGATCY 4 cut(s) 259, 1164, 2606, 2947
BsuRI GGCC 5 cut(s) 601, 919, 2805, 2884, 2962
BtgI CCRYGG 2 cut(s) 2095, 2632
BtsCI GGATG 6 cut(s) 442, 907, 1096, 1187, 1543, 2764
BtsI GCAGTG 2 cut(s) 1983, 2060
BtsIMutI CAGTG 7 cut(s) 1196, 1683, 1712, 1983, 2060, 2328, 2422
Cac8I GCNNGC 3 cut(s) 953, 1141, 2548
CaiI CAGNNNCTG 1 cut(s) 2468
CciI TCATGA 1 cut(s) 808
CfoI GCGC 2 cut(s) 1436, 2901
Cfr13I GGNCC 6 cut(s) 781, 917, 1225, 1653, 1981, 2804
CspCI CAANNNNNGTGG 4 cut(s) 195, 230, 2304, 2339
DraI TTTAAA 2 cut(s) 228, 864
DrdI GACNNNNNNGTC 1 cut(s) 1535
DseDI GACNNNNNNGTC 1 cut(s) 1535
EaeI YGGCCR 1 cut(s) 2960
Eam1104I CTCTTC 2 cut(s) 1164, 2925
EarI CTCTTC 2 cut(s) 1164, 2925
Eco130I CCWWGG 4 cut(s) 596, 1656, 2095, 2632
Eco147I AGGCCT 1 cut(s) 2884
Eco24I GRGCYC 1 cut(s) 881
Eco31I GGTCTC 2 cut(s) 1379, 2405
Eco47I GGWCC 4 cut(s) 781, 1225, 1653, 1981
Eco47III AGCGCT 1 cut(s) 2900
Eco57I CTGAAG 2 cut(s) 1145, 2148
Eco88I CYCGRG 1 cut(s) 2198
EcoO109I RGGNCCY 4 cut(s) 781, 917, 1225, 1653
EcoRI GAATTC 4 cut(s) 125, 270, 2123, 2364
EcoRII CCWGG 3 cut(s) 736, 2359, 2806
EcoT14I CCWWGG 4 cut(s) 596, 1656, 2095, 2632
EcoT22I ATGCAT 1 cut(s) 1614
EcoT38I GRGCYC 1 cut(s) 881
ErhI CCWWGG 4 cut(s) 596, 1656, 2095, 2632
FalI AAGNNNNNCTT 2 cut(s) 2146, 2178
FaqI GGGAC 2 cut(s) 1135, 1238
FauI CCCGC 1 cut(s) 2377
FauNDI CATATG 1 cut(s) 520
FbaI TGATCA 3 cut(s) 1333, 1692, 2047
FblI GTMKAC 1 cut(s) 2628
Fnu4HI GCNGC 9 cut(s) 197, 245, 845, 1247, 1432, 2457, 2526, 2714, 2902
FokI GGATG 6 cut(s) 449, 894, 1103, 1174, 1530, 2751
FriOI GRGCYC 1 cut(s) 881
Fsp4HI GCNGC 9 cut(s) 197, 245, 845, 1247, 1432, 2457, 2526, 2714, 2902
FspBI CTAG 7 cut(s) 6, 375, 465, 597, 882, 956, 2867
GlaI GCGC 2 cut(s) 1435, 2900
GluI GCNGC 9 cut(s) 197, 245, 845, 1247, 1432, 2457, 2526, 2714, 2902
GsaI CCCAGC 1 cut(s) 2092
GsuI CTGGAG 8 cut(s) 964, 1580, 1736, 1859, 2445, 2752, 2829, 2996
HaeII RGCGCY 1 cut(s) 2902
HaeIII GGCC 5 cut(s) 601, 919, 2805, 2884, 2962
HapII CCGG 1 cut(s) 2945
HhaI GCGC 2 cut(s) 1436, 2901
Hin6I GCGC 2 cut(s) 1434, 2899
HinP1I GCGC 2 cut(s) 1434, 2899
HincII GTYRAC 2 cut(s) 1759, 3003
HindII GTYRAC 2 cut(s) 1759, 3003
HinfI GANTC 8 cut(s) 293, 497, 1625, 2029, 2245, 2465, 2746, 2906
HpaII CCGG 1 cut(s) 2945
HphI GGTGA 8 cut(s) 506, 752, 1698, 1805, 2323, 2424, 2753, 2854
Hpy166II GTNNAC 7 cut(s) 110, 1133, 1759, 1981, 2058, 2629, 3003
Hpy8I GTNNAC 7 cut(s) 110, 1133, 1759, 1981, 2058, 2629, 3003
HpyCH4III ACNGT 7 cut(s) 36, 396, 926, 1200, 2110, 2626, 2827
HpyCH4IV ACGT 2 cut(s) 1252, 2992
HpyF10VI GCNNNNNNNGC 4 cut(s) 2378, 2531, 2713, 2828
HpySE526I ACGT 2 cut(s) 1252, 2992
HspAI GCGC 2 cut(s) 1434, 2899
Ksp22I TGATCA 3 cut(s) 1333, 1692, 2047
LmnI GCTCC 3 cut(s) 745, 983, 2464
Lsp1109I GCAGC 7 cut(s) 208, 256, 1258, 1443, 2468, 2512, 2888
MaeI CTAG 7 cut(s) 6, 375, 465, 597, 882, 956, 2867
MaeII ACGT 2 cut(s) 1252, 2992
MbiI CCGCTC 1 cut(s) 2713
MflI RGATCY 4 cut(s) 259, 1164, 2606, 2947
MhlI GDGCHC 1 cut(s) 881
MlyI GAGTC 3 cut(s) 1619, 2474, 2900
MmeI TCCRAC 1 cut(s) 1064
Mph1103I ATGCAT 1 cut(s) 1614
MroXI GAANNNNTTC 2 cut(s) 1892, 2262
MslI CAYNNNNRTG 2 cut(s) 1278, 2769
MspA1I CMGCKG 3 cut(s) 332, 984, 1753
MspI CCGG 1 cut(s) 2945
MspR9I CCNGG 3 cut(s) 738, 2361, 2808
Mva1269I GAATGC 1 cut(s) 1027
MvaI CCWGG 3 cut(s) 738, 2361, 2808
MwoI GCNNNNNNNGC 4 cut(s) 2378, 2531, 2713, 2828
NcoI CCATGG 2 cut(s) 2095, 2632
NdeI CATATG 1 cut(s) 520
NlaIV GGNNCC 4 cut(s) 918, 1226, 1983, 2949
NmuCI GTSAC 7 cut(s) 68, 181, 901, 1010, 2277, 2311, 2482
NsiI ATGCAT 1 cut(s) 1614
NspI RCATGY 7 cut(s) 955, 1139, 1417, 1501, 1534, 1616, 2864
NspV TTCGAA 2 cut(s) 409, 1896
PaeI GCATGC 1 cut(s) 955
PagI TCATGA 1 cut(s) 808
PceI AGGCCT 1 cut(s) 2884
PciI ACATGT 1 cut(s) 1530
PcsI WCGNNNNNNNCGW 1 cut(s) 1644
PctI GAATGC 1 cut(s) 1027
PdmI GAANNNNTTC 2 cut(s) 1892, 2262
PfeI GAWTC 5 cut(s) 293, 497, 2029, 2245, 2746
PflMI CCANNNNNTGG 1 cut(s) 608
PkrI GCNGC 9 cut(s) 198, 246, 846, 1248, 1433, 2458, 2527, 2715, 2903
PleI GAGTC 3 cut(s) 1619, 2473, 2900
PpsI GAGTC 3 cut(s) 1619, 2473, 2900
Ppu21I YACGTR 1 cut(s) 2993
PpuMI RGGWCCY 3 cut(s) 781, 1225, 1653
PscI ACATGT 1 cut(s) 1530
PshAI GACNNNNGTC 1 cut(s) 963
Psp5II RGGWCCY 3 cut(s) 781, 1225, 1653
Psp6I CCWGG 3 cut(s) 736, 2359, 2806
PspFI CCCAGC 1 cut(s) 2088
PspGI CCWGG 3 cut(s) 736, 2359, 2806
PspN4I GGNNCC 4 cut(s) 918, 1226, 1983, 2949
PspPI GGNCC 6 cut(s) 781, 917, 1225, 1653, 1981, 2804
PspPPI RGGWCCY 3 cut(s) 781, 1225, 1653
PstI CTGCAG 4 cut(s) 147, 246, 575, 2491
PstNI CAGNNNCTG 1 cut(s) 2468
PsuI RGATCY 4 cut(s) 259, 1164, 2606, 2947
PvuII CAGCTG 2 cut(s) 332, 1753
RseI CAYNNNNRTG 2 cut(s) 1278, 2769
SatI GCNGC 9 cut(s) 197, 245, 845, 1247, 1432, 2457, 2526, 2714, 2902
Sau96I GGNCC 6 cut(s) 781, 917, 1225, 1653, 1981, 2804
SchI GAGTC 3 cut(s) 1619, 2474, 2900
ScrFI CCNGG 3 cut(s) 738, 2361, 2808
SduI GDGCHC 1 cut(s) 881
SfcI CTRYAG 6 cut(s) 143, 242, 571, 2436, 2487, 2826
SfuI TTCGAA 2 cut(s) 409, 1896
SinI GGWCC 4 cut(s) 781, 1225, 1653, 1981
SmiI ATTTAAAT 1 cut(s) 228
SmiMI CAYNNNNRTG 2 cut(s) 1278, 2769
SmlI CTYRAG 2 cut(s) 2118, 2134
SmoI CTYRAG 2 cut(s) 2118, 2134
SpeI ACTAGT 1 cut(s) 5
SphI GCATGC 1 cut(s) 955
SseBI AGGCCT 1 cut(s) 2884
SsiI CCGC 8 cut(s) 40, 434, 844, 984, 2370, 2381, 2713, 3022
SspI AATATT 3 cut(s) 103, 591, 2177
SspMI CTAG 7 cut(s) 6, 375, 465, 597, 882, 956, 2867
StuI AGGCCT 1 cut(s) 2884
StyD4I CCNGG 3 cut(s) 736, 2359, 2806
StyI CCWWGG 4 cut(s) 596, 1656, 2095, 2632
SwaI ATTTAAAT 1 cut(s) 228
TaaI ACNGT 7 cut(s) 36, 396, 926, 1200, 2110, 2626, 2827
TaiI ACGT 2 cut(s) 1255, 2995
TaqI TCGA 5 cut(s) 409, 672, 1647, 1896, 2241
TatI WGTACW 4 cut(s) 109, 1615, 1992, 2057
TauI GCSGC 2 cut(s) 847, 2716
TfiI GAWTC 5 cut(s) 293, 497, 2029, 2245, 2746
TscAI CASTG 7 cut(s) 1203, 1683, 1719, 1983, 2060, 2328, 2422
TseFI GTSAC 7 cut(s) 68, 181, 901, 1010, 2277, 2311, 2482
TseI GCWGC 7 cut(s) 196, 244, 1246, 1431, 2456, 2525, 2901
Tsp45I GTSAC 7 cut(s) 68, 181, 901, 1010, 2277, 2311, 2482
TspGWI ACGGA 2 cut(s) 149, 655
TspRI CASTG 7 cut(s) 1203, 1683, 1719, 1983, 2060, 2328, 2422
Van91I CCANNNNNTGG 1 cut(s) 608
VpaK11BI GGWCC 4 cut(s) 781, 1225, 1653, 1981
XceI RCATGY 7 cut(s) 955, 1139, 1417, 1501, 1534, 1616, 2864
XmaJI CCTAGG 1 cut(s) 596
XmiI GTMKAC 1 cut(s) 2628
XmnI GAANNNNTTC 2 cut(s) 1892, 2262
XspI CTAG 7 cut(s) 6, 375, 465, 597, 882, 956, 2867
Zsp2I ATGCAT 1 cut(s) 1614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.