pycom13g20420

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012803
Physical Location & Seq
Forward (+)
16500286 .. 16505100
4815 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g20420.5

Sequence Viewer

Length: 3579 bp
ATGACTGGTGAGGTTCCTGGTACTTCTGGTCGACAGTTTTGCAGAGATACAACCAATGATGTTCGAAGTGACAGAAATGCTGCGAACGAAAATGTGACTAATATTTGTCTACAGACCGGTGAGGAATTTTCAACCGAGTTTCTGCAGGATCGTATTGCACAAAGAAGATTAGCCCCTGTACTGACTGGCATTGATCTGCGCCAGTCGGAACGGTTGGGTTCTAATATAAACCAAAACCCTAAACTGCTTTACGAGGATCTTGGTGGCGTTATGGGGATGAAGAGAATAGATTCCGATTGCAATTCAGAATTCTCGGATTTTAGTCCAGTTGCTCCGAAAGGTTTTCTAGCGGACACAGAGAAAAATGTTTCTCCTAGTAACATGAGCAGATACCACTTGGAATATGGTGCCAAGGGAAAAGCATCGAGTAAGTTTGCTGCTGAAGTTAATCACAATCGGGTTATACCAAGACCAACTACTCCACCTTTTTATGTTGTGGACTCCCCTCAATCATACACTCCTTATGGTCAAGTATTTGCTGAAGGTTCTTTCTCTTTCAAAATGAAATTTCTCTGCAGCTTTGGGGGAAGAATATTACCTCGGCCAAATGATGGGAAACTCAGATATGTAGGTGGAGATACACGGATTTTATCTATTCGTGAAGGCACCAATTTTGAGGAACTAATGAAGAAGACATTTGCAATTTGCAACCAACATCACACTATCAAGTACCAGCTTCCCGGTGAGGATCTTGATGCACTCGTATCTGTTTGTTCCGATGAGGACCTTCTTCATATGATAGAGGAATATCATGAGCTAGAGAGAACAGAGGGTTCTCAAAGACTGAGGCTCTTCCTTGTACCTTTAACTGAAACAGAGAGCCCAAGTTCTGTCGAGGCAAGGATTACACATCCAATCGGTGCTGACTGCGAATTTGTTTTCGCTGTTAATGGTATGTTAGACCCAAGTCCTCAGAAAAGCTCTAGCGAGCAAACTTTGGCCAGCCAGACTGGAAATCCATCAGATTATAGTCCAACCTTTCATAGAGACTCTCCTACCGGTGCATATTTTGAGACCAAAGATTATAGCCCTAATTCCTCAAGTGCAGTGGGCACATTACCTAAACCTGCTCCTCAGTTCTTAGCTACTCTTCAGATCCCAAAGAAGTCTTTTAATCAATCCCCTCCCATATCCCCGGTTCCACTTCAACACAGAGATCCCAAGAGTTCCAATGTGCAGCTTTATTTGGACCGATCATATTGTGAAGGTAATGCAGGCATTGCTCCTTATGGTATGGAGAAATATCCATGTGACAATACCTATTATCTTGATGCGGGAAGTTATAACAATAATCTTCATCATGGGCCTCCATTGTTAAATCATCATCATCATCATAACCAATATTTGGCTGAAACTTATAGGGCTAAAAAGTCTCACATTGTGCACTCCCACAATCGTAGTTTTAGCGAAAATTTTGTGCCTTCTGCACAGTATGGCAAAAGTAGTATGGAATCTGAGAGACTTGTGATTCTGGAAAAGGCCCTCCATTCGGAAAAGTTTCTCTCTCGTCCTGAAGATAGAGAGGGACTATCTTCAGGGTCCGGGGAAAGAGATGCTTCTTACCACAGAATAATGCATGCAGTACATTCTGACTCTCAGTTGCAGGAACATGTTCAGAGGTCCACCGAAGGAGAAGTTATCCCAGTGTCGTCATTGAAATACAGACTAGAAAAATCACCTTCGTTGAAGATACAAAGGTCCTCGCAAGAACGGCCTGTGCAACAGGAAGTGATTCTTGATGGGAAACATCAAATTGCTAAACACGGTAATGAATTCACAATCAGGAAACCTGACCAATGTAAAGTGGAACGAGGTCTGGAGATGTTGAACCAGACGGACAGGAATGACACTTGTGCTGATAAAAAATGGAAACACTTTGAAGGAAATGTTGAAGTTACATCAAATGATATTGTCATGGAATTTAAGAAGGAACAAAATGCAAACTGCCTTGTCAGTGATTGCTTAGCTATGGAACATTCACAATTCCCTGAAAGTGGGATTTTTGGTTCATCAAACCCCGAAACTGAAGGTTCTGAAGATACTACGGGCCCCCAAGGCTATGGATTTGACACAACTTCTCAGTTTTCTCTTAGGAGCCAAAACTGTATTAGAGATCAGCGGTGTGCTACAGTTGAGACAGTAAGCAGTCAAACAGTTTCTCATCAGTCACGTGAACTACCACCTGTTGCATCTCAGGAGTTTTTAGTCTCAACATCTAAATTGGCTACAACTGCTGCACCAGACAGAGAATTTTCTCTCCATGATAAAGAGTCAATAAATTATCCTGAGTATTTGGTTGAAAACATATATCTCAGCAGACAGTCTTCTGAAGTAACCAACTGTGAAGGTGCTATCCCTGCCCAATCACAGCCACTGGATAATCGGCATGACAACAAGGTGACGGACTCAGTGATTATTGTAGAAGACTTGACTAATAATACACCACCCGGTATCCCTACCTCCAAAGTGGTCTATAACGTCTCACATATAGAAGATGAGGCCAGTGATGATTGTTCATCAACTGGAGAAACAGAGACTGGAAGTTCAGCTACAGCATCTCATAACAAGGCTGTGACAGCTGACAGAAGTCATAGGCATGACACTATTAGCAATGTTGCAATAGCCGAAATGGAAGCAGGCATCTATGGTTTGCAGATCATAAAGAATGATGATCTCGAAGAACTGCAAGAATTAGGATCTGGTACATATGGAACTGTCTATCATGGAAAGTGGAGAGGAACAGATGTTGCAATTAAGAGGATAAAAAAGAGTTGTTTCTCCGGCAGATCATCAGAGCAAGATCGACTGACTAAGGACTTCTGGAGAGAGGCAAAGATTCTGTCAACCCTTCACCATCCCAATGTTGTAGCATTCTATGGCGTGGTGCCTGATGGGCCTGGGGGGACGTTAGCAACTGTAGCTGAATTCATGGTGAATGGGTCGTTGAGGCATGTCCTAATAAGGAAAGATAGAGTGCTTGATCGTCAAAAGAGGCTCATAATCTTAATGGACACAGTTTTTGGCATGGAGTATTTGCATTTGAAAAATATTGTTCACTTTGATTTGAAGTGTGACAATTTACTAGTTAATTTGAGGGATCCCGAACGGCCCATATGCAAGGTTGGAGATTTTGGTTTGTCAAGAATTAAGCGCAACACACTAGTATCTGGTGGTGTTCGTGGAACCCTTCCGTGGATGGCACCAGAACTATTAAATGGTAGCAGTAACCGGGTCTCTGAGAAGGTTGATGTCTTCTCGTTCGGTATTGTAATGTGGGAGATCCTGACTGGGGAGGAACCGTACGCTAACATGCACTGTGGTGCTATCATAGGGGGAATTGTAAATAACACACTGAGGCCTTCTATTCCAAAGCGTTGTGATTCTGAATGGAAAAAACTAATGGAACAGTGCTGGGCGCCGGATCCTGCTGATCGTCCATCATTCACAGAGATAACACATAGGCTGCGTGACATGTCAATGGCACTCCAGAAAAAGCGGCCCAGTCTTGCAAGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1193

Amino Acids

133.26

Weight (kDa)

5.97

Isoelectric Point (pI)

48.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04700 AT1G04700 AT1G04700
fragaria_vesca FvH4_4g05890
malus_domestica MD13G1231300.v1.1 MD16G1235800.v1.1
prunus_persica Prupe.1G061200_v2.0.a1
pyrus_communis pycom13g20420
rosa_chinensis RchiOBHm_Chr4g0396991
rosa_laevigata RLG00000009461
rosa_multiflora Rmu_sc0002963.1_g000016
rosa_roxburghii Rroxscaffold_5G00341980
rosa_rugosa Rorug03G0346000
rosa_samantha Rh4AG075900 Rh4BG072300 Rh4CG080600 Rh4DG069700
rosa_wichuraiana Rw4G006090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1344
Acc36I ACCTGC 1 cut(s) 1135
AccB1I GGYRCC 5 cut(s) 407, 665, 2944, 3259, 3475
AccB7I CCANNNNNTGG 2 cut(s) 611, 1405
AccI GTMKAC 2 cut(s) 31, 109
AciI CCGC 4 cut(s) 350, 1334, 2179, 3556
AcoI YGGCCR 2 cut(s) 602, 999
AcsI RAATTY 9 cut(s) 125, 308, 566, 932, 1471, 1832, 1979, 2309, 2984
AcuI CTGAAG 8 cut(s) 462, 561, 1136, 1578, 1593, 2106, 2115, 2409
AcvI CACGTG 1 cut(s) 2231
AcyI GRCGYC 1 cut(s) 3476
AdeI CACNNNGTG 1 cut(s) 1441
AfaI GTAC 7 cut(s) 22, 180, 731, 861, 1644, 2764, 3362
AfiI CCNNNNNNNGG 6 cut(s) 611, 1405, 1549, 2054, 3252, 3349
AflIII ACRYGT 2 cut(s) 1669, 3531
AgeI ACCGGT 2 cut(s) 116, 1058
AhlI ACTAGT 2 cut(s) 3142, 3220
AjnI CCWGG 2 cut(s) 16, 2956
AleI CACNNNNGTG 1 cut(s) 3378
AloI GAACNNNNNNTCC 2 cut(s) 2050, 2082
Alw21I GWGCWC 1 cut(s) 1446
Alw26I GTCTC 9 cut(s) 1041, 1067, 1437, 1513, 2189, 2272, 2545, 2588, 3298
Alw44I GTGCAC 1 cut(s) 1442
AlwNI CAGNNNCTG 2 cut(s) 2434, 2597
ApaI GGGCCC 1 cut(s) 2111
ApaLI GTGCAC 1 cut(s) 1442
ApeKI GCWGC 6 cut(s) 80, 437, 576, 1237, 2294, 3523
ApoI RAATTY 9 cut(s) 125, 308, 566, 932, 1471, 1832, 1979, 2309, 2984
ArsI GACNNNNNNTTYG 6 cut(s) 917, 949, 2885, 2917, 3062, 3094
AsiGI ACCGGT 2 cut(s) 116, 1058
Asp700I GAANNNNTTC 4 cut(s) 289, 1557, 1671, 1791
AspLEI GCGC 3 cut(s) 201, 3213, 3478
AsuC2I CCSGG 5 cut(s) 741, 1196, 1603, 2508, 3290
AsuHPI GGTGA 7 cut(s) 20, 131, 755, 1728, 2470, 2903, 3004
AsuII TTCGAA 1 cut(s) 64
AvaII GGWCC 5 cut(s) 784, 1249, 1599, 1680, 1758
BaeGI GKGCMC 3 cut(s) 1115, 1446, 2111
BaeI ACNNNNGTAYC 4 cut(s) 1742, 1775, 3207, 3240
BalI TGGCCA 1 cut(s) 1001
BamHI GGATCC 2 cut(s) 3157, 3481
BanI GGYRCC 5 cut(s) 407, 665, 2944, 3259, 3475
BanII GRGCYC 2 cut(s) 884, 2111
BarI GAAGNNNNNNTAC 2 cut(s) 2593, 2625
BbrPI CACGTG 1 cut(s) 2231
BbsI GAAGAC 4 cut(s) 698, 2376, 2490, 3304
Bbv12I GWGCWC 1 cut(s) 1446
BbvI GCAGC 6 cut(s) 67, 424, 588, 1249, 2281, 3510
BccI CCATC 7 cut(s) 605, 1027, 1793, 2922, 2945, 3250, 3505
BceAI ACGGC 2 cut(s) 1787, 3182
BcgI CGANNNNNNTGC 2 cut(s) 21, 55
BciT130I CCWGG 2 cut(s) 18, 2958
BciVI GTATCC 1 cut(s) 2522
BcnI CCSGG 5 cut(s) 741, 1196, 1603, 2508, 3290
BcoDI GTCTC 9 cut(s) 1041, 1067, 1437, 1513, 2189, 2272, 2545, 2588, 3298
BcuI ACTAGT 2 cut(s) 3142, 3220
BfaI CTAG 7 cut(s) 347, 375, 818, 984, 1727, 3143, 3221
BfmI CTRYAG 6 cut(s) 110, 143, 574, 2187, 2610, 2976
BfoI RGCGCY 1 cut(s) 3479
BfuAI ACCTGC 1 cut(s) 1135
BfuI GTATCC 1 cut(s) 2522
BglI GCCNNNNNGGC 2 cut(s) 2115, 2953
BisI GCNGC 7 cut(s) 81, 438, 577, 1238, 2295, 3524, 3557
BlpI GCTNAGC 1 cut(s) 2023
BlsI GCNGC 7 cut(s) 82, 439, 578, 1239, 2296, 3525, 3558
Bme1390I CCNGG 7 cut(s) 18, 741, 1196, 1603, 2508, 2958, 3290
Bme18I GGWCC 5 cut(s) 784, 1249, 1599, 1680, 1758
BmrFI CCNGG 7 cut(s) 18, 741, 1196, 1603, 2508, 2958, 3290
BmrI ACTGGG 3 cut(s) 1697, 3357, 3555
BmsI GCATC 7 cut(s) 431, 745, 1321, 1603, 2258, 2624, 2709
BmuI ACTGGG 3 cut(s) 1697, 3357, 3555
BoxI GACNNNNGTC 2 cut(s) 966, 2646
BpiI GAAGAC 4 cut(s) 698, 2376, 2490, 3304
BpmI CTGGAG 4 cut(s) 1898, 2604, 2902, 3530
Bpu1102I GCTNAGC 1 cut(s) 2023
Bpu14I TTCGAA 1 cut(s) 64
BpuEI CTTGAG 1 cut(s) 1084
BpuMI CCSGG 5 cut(s) 741, 1196, 1603, 2508, 3290
BsaAI YACGTR 1 cut(s) 2231
BsaHI GRCGYC 1 cut(s) 3476
BsaI GGTCTC 2 cut(s) 1067, 3298
BsaJI CCNNGG 7 cut(s) 411, 599, 1194, 1602, 2113, 2957, 3251
BsaWI WCCGGW 2 cut(s) 116, 1058
BsaXI ACNNNNNCTCC 2 cut(s) 3344, 3374
Bsc4I CCNNNNNNNGG 6 cut(s) 611, 1405, 1549, 2054, 3252, 3349
Bse118I RCCGGY 2 cut(s) 116, 1058
Bse3DI GCAATG 2 cut(s) 1278, 2677
BseBI CCWGG 2 cut(s) 18, 2958
BseDI CCNNGG 7 cut(s) 411, 599, 1194, 1602, 2113, 2957, 3251
BseGI GGATG 4 cut(s) 282, 910, 2914, 3261
BseLI CCNNNNNNNGG 6 cut(s) 611, 1405, 1549, 2054, 3252, 3349
BseMI GCAATG 2 cut(s) 1278, 2677
BseRI GAGGAG 1 cut(s) 1122
BseSI GKGCMC 3 cut(s) 1115, 1446, 2111
BseXI GCAGC 6 cut(s) 67, 424, 588, 1249, 2281, 3510
BseYI CCCAGC 1 cut(s) 3471
BsgI GTGCAG 4 cut(s) 1125, 1256, 1470, 2280
BshNI GGYRCC 5 cut(s) 407, 665, 2944, 3259, 3475
BshTI ACCGGT 2 cut(s) 116, 1058
BsiHKAI GWGCWC 1 cut(s) 1446
BsiSI CCGG 9 cut(s) 117, 741, 1059, 1196, 1602, 2508, 2841, 3289, 3479
BsiWI CGTACG 1 cut(s) 3360
BslFI GGGAC 2 cut(s) 1599, 2977
BslI CCNNNNNNNGG 6 cut(s) 611, 1405, 1549, 2054, 3252, 3349
BsmAI GTCTC 9 cut(s) 1041, 1067, 1437, 1513, 2189, 2272, 2545, 2588, 3298
BsmBI CGTCTC 1 cut(s) 2545
BsmFI GGGAC 2 cut(s) 1599, 2977
BsmI GAATGC 1 cut(s) 2930
Bso31I GGTCTC 2 cut(s) 1067, 3298
Bsp119I TTCGAA 1 cut(s) 64
Bsp120I GGGCCC 1 cut(s) 2107
Bsp1286I GDGCHC 4 cut(s) 884, 1115, 1446, 2111
Bsp1720I GCTNAGC 1 cut(s) 2023
BspACI CCGC 4 cut(s) 350, 1334, 2179, 3556
BspHI TCATGA 1 cut(s) 811
BspMAI CTGCAG 2 cut(s) 147, 578
BspMI ACCTGC 1 cut(s) 1135
BspQI GCTCTTC 1 cut(s) 857
BspT104I TTCGAA 1 cut(s) 64
BspT107I GGYRCC 5 cut(s) 407, 665, 2944, 3259, 3475
BspTNI GGTCTC 2 cut(s) 1067, 3298
BsrDI GCAATG 2 cut(s) 1278, 2677
BsrFI RCCGGY 2 cut(s) 116, 1058
BssAI RCCGGY 2 cut(s) 116, 1058
BssECI CCNNGG 7 cut(s) 411, 599, 1194, 1602, 2113, 2957, 3251
BssNI GRCGYC 1 cut(s) 3476
BssT1I CCWWGG 2 cut(s) 411, 2113
Bst2UI CCWGG 2 cut(s) 18, 2958
Bst6I CTCTTC 3 cut(s) 275, 857, 1155
BstACI GRCGYC 1 cut(s) 3476
BstAPI GCANNNNNTGC 1 cut(s) 1280
BstBAI YACGTR 1 cut(s) 2231
BstBI TTCGAA 1 cut(s) 64
BstC8I GCNNGC 5 cut(s) 989, 1003, 1276, 1638, 2698
BstDSI CCRYGG 1 cut(s) 3251
BstF5I GGATG 4 cut(s) 282, 910, 2914, 3261
BstH2I RGCGCY 1 cut(s) 3479
BstHHI GCGC 3 cut(s) 201, 3213, 3478
BstMAI GTCTC 9 cut(s) 1041, 1067, 1437, 1513, 2189, 2272, 2545, 2588, 3298
BstMWI GCNNNNNNNGC 8 cut(s) 1280, 1771, 2115, 2291, 2417, 2636, 2953, 2978
BstNI CCWGG 2 cut(s) 18, 2958
BstNSI RCATGY 5 cut(s) 1640, 1673, 3014, 3373, 3535
BstPAI GACNNNNGTC 2 cut(s) 966, 2646
BstSCI CCNGG 7 cut(s) 16, 739, 1194, 1601, 2506, 2956, 3288
BstSFI CTRYAG 6 cut(s) 110, 143, 574, 2187, 2610, 2976
BstSLI GKGCMC 3 cut(s) 1115, 1446, 2111
BstV1I GCAGC 6 cut(s) 67, 424, 588, 1249, 2281, 3510
BstV2I GAAGAC 4 cut(s) 698, 2376, 2490, 3304
BstX2I RGATCY 8 cut(s) 256, 748, 1155, 1216, 2756, 3157, 3339, 3481
BstXI CCANNNNNNTGG 1 cut(s) 2120
BstYI RGATCY 8 cut(s) 256, 748, 1155, 1216, 2756, 3157, 3339, 3481
BsuI GTATCC 1 cut(s) 2522
BtgI CCRYGG 1 cut(s) 3251
BtsCI GGATG 4 cut(s) 282, 910, 2914, 3261
BtsI GCAGTG 1 cut(s) 1113
BtsIMutI CAGTG 9 cut(s) 1113, 1710, 2020, 2432, 2475, 2569, 3373, 3410, 3473
BveI ACCTGC 1 cut(s) 1135
Cac8I GCNNGC 5 cut(s) 989, 1003, 1276, 1638, 2698
CaiI CAGNNNCTG 2 cut(s) 2434, 2597
CciI TCATGA 1 cut(s) 811
CfoI GCGC 3 cut(s) 201, 3213, 3478
Cfr10I RCCGGY 2 cut(s) 116, 1058
Csp6I GTAC 7 cut(s) 21, 179, 730, 860, 1643, 2763, 3361
CspAI ACCGGT 2 cut(s) 116, 1058
CspCI CAANNNNNGTGG 2 cut(s) 1089, 1124
CviQI GTAC 7 cut(s) 21, 179, 730, 860, 1643, 2763, 3361
DinI GGCGCC 1 cut(s) 3477
DraIII CACNNNGTG 1 cut(s) 1441
EaeI YGGCCR 2 cut(s) 602, 999
Eam1104I CTCTTC 3 cut(s) 275, 857, 1155
EarI CTCTTC 3 cut(s) 275, 857, 1155
Eco130I CCWWGG 2 cut(s) 411, 2113
Eco147I AGGCCT 1 cut(s) 3418
Eco24I GRGCYC 2 cut(s) 884, 2111
Eco31I GGTCTC 2 cut(s) 1067, 3298
Eco47I GGWCC 5 cut(s) 784, 1249, 1599, 1680, 1758
Eco57I CTGAAG 8 cut(s) 462, 561, 1136, 1578, 1593, 2106, 2115, 2409
Eco72I CACGTG 1 cut(s) 2231
EcoO109I RGGNCCY 4 cut(s) 784, 1540, 1758, 2108
EcoRI GAATTC 3 cut(s) 308, 1832, 2984
EcoRII CCWGG 2 cut(s) 16, 2956
EcoT14I CCWWGG 2 cut(s) 411, 2113
EcoT22I ATGCAT 1 cut(s) 1638
EcoT38I GRGCYC 2 cut(s) 884, 2111
EgeI GGCGCC 1 cut(s) 3477
EheI GGCGCC 1 cut(s) 3477
ErhI CCWWGG 2 cut(s) 411, 2113
Esp3I CGTCTC 1 cut(s) 2545
FalI AAGNNNNNCTT 4 cut(s) 1600, 1632, 1779, 1811
FaqI GGGAC 2 cut(s) 1599, 2977
FauI CCCGC 1 cut(s) 1327
FauNDI CATATG 3 cut(s) 795, 2767, 3173
FblI GTMKAC 2 cut(s) 31, 109
Fnu4HI GCNGC 7 cut(s) 81, 438, 577, 1238, 2295, 3524, 3557
FokI GGATG 4 cut(s) 289, 897, 2901, 3268
FriOI GRGCYC 2 cut(s) 884, 2111
Fsp4HI GCNGC 7 cut(s) 81, 438, 577, 1238, 2295, 3524, 3557
FspBI CTAG 7 cut(s) 347, 375, 818, 984, 1727, 3143, 3221
GlaI GCGC 3 cut(s) 200, 3212, 3477
GluI GCNGC 7 cut(s) 81, 438, 577, 1238, 2295, 3524, 3557
GsaI CCCAGC 1 cut(s) 3475
GsuI CTGGAG 4 cut(s) 1898, 2604, 2902, 3530
HaeII RGCGCY 1 cut(s) 3479
HapII CCGG 9 cut(s) 117, 741, 1059, 1196, 1602, 2508, 2841, 3289, 3479
HhaI GCGC 3 cut(s) 201, 3213, 3478
Hin1I GRCGYC 1 cut(s) 3476
Hin6I GCGC 3 cut(s) 199, 3211, 3476
HinP1I GCGC 3 cut(s) 199, 3211, 3476
HincII GTYRAC 2 cut(s) 32, 2904
HindII GTYRAC 2 cut(s) 32, 2904
HpaII CCGG 9 cut(s) 117, 741, 1059, 1196, 1602, 2508, 2841, 3289, 3479
HphI GGTGA 7 cut(s) 20, 131, 755, 1728, 2470, 2903, 3004
Hpy166II GTNNAC 8 cut(s) 32, 110, 499, 1444, 1683, 2234, 2904, 3115
Hpy8I GTNNAC 8 cut(s) 32, 110, 499, 1444, 1683, 2234, 2904, 3115
HpyCH4IV ACGT 3 cut(s) 2230, 2538, 2966
HpyF10VI GCNNNNNNNGC 8 cut(s) 1280, 1771, 2115, 2291, 2417, 2636, 2953, 2978
HpySE526I ACGT 3 cut(s) 2230, 2538, 2966
Hsp92I GRCGYC 1 cut(s) 3476
HspAI GCGC 3 cut(s) 199, 3211, 3476
KasI GGCGCC 1 cut(s) 3475
LguI GCTCTTC 1 cut(s) 857
LmnI GCTCC 4 cut(s) 337, 1135, 1288, 2154
Lsp1109I GCAGC 6 cut(s) 67, 424, 588, 1249, 2281, 3510
LweI GCATC 7 cut(s) 431, 745, 1321, 1603, 2258, 2624, 2709
MaeI CTAG 7 cut(s) 347, 375, 818, 984, 1727, 3143, 3221
MaeII ACGT 3 cut(s) 2230, 2538, 2966
MflI RGATCY 8 cut(s) 256, 748, 1155, 1216, 2756, 3157, 3339, 3481
MhlI GDGCHC 4 cut(s) 884, 1115, 1446, 2111
MlsI TGGCCA 1 cut(s) 1001
MluNI TGGCCA 1 cut(s) 1001
Mly113I GGCGCC 1 cut(s) 3476
MlyI GAGTC 5 cut(s) 494, 1043, 1646, 2339, 2459
MmeI TCCRAC 3 cut(s) 186, 1058, 3163
Mox20I TGGCCA 1 cut(s) 1001
Mph1103I ATGCAT 1 cut(s) 1638
MroXI GAANNNNTTC 4 cut(s) 289, 1557, 1671, 1791
MscI TGGCCA 1 cut(s) 1001
MslI CAYNNNNRTG 5 cut(s) 1827, 2655, 2919, 3378, 3536
Msp20I TGGCCA 1 cut(s) 1001
MspA1I CMGCKG 2 cut(s) 2179, 2639
MspI CCGG 9 cut(s) 117, 741, 1059, 1196, 1602, 2508, 2841, 3289, 3479
MspR9I CCNGG 7 cut(s) 18, 741, 1196, 1603, 2508, 2958, 3290
Mva1269I GAATGC 1 cut(s) 2930
MvaI CCWGG 2 cut(s) 18, 2958
MwoI GCNNNNNNNGC 8 cut(s) 1280, 1771, 2115, 2291, 2417, 2636, 2953, 2978
NarI GGCGCC 1 cut(s) 3476
NciI CCSGG 5 cut(s) 741, 1196, 1603, 2508, 3290
NdeI CATATG 3 cut(s) 795, 2767, 3173
NmeAIII GCCGAG 1 cut(s) 580
NmuCI GTSAC 8 cut(s) 68, 94, 1310, 2226, 2458, 2632, 3131, 3527
NsiI ATGCAT 1 cut(s) 1638
NspI RCATGY 5 cut(s) 1640, 1673, 3014, 3373, 3535
NspV TTCGAA 1 cut(s) 64
OliI CACNNNNGTG 1 cut(s) 3378
PaeI GCATGC 1 cut(s) 1640
PagI TCATGA 1 cut(s) 811
PceI AGGCCT 1 cut(s) 3418
PciI ACATGT 2 cut(s) 1669, 3531
PciSI GCTCTTC 1 cut(s) 857
PctI GAATGC 1 cut(s) 2930
PdmI GAANNNNTTC 4 cut(s) 289, 1557, 1671, 1791
PfeI GAWTC 6 cut(s) 290, 1511, 1528, 1792, 2896, 3440
Pfl23II CGTACG 1 cut(s) 3360
PflMI CCANNNNNTGG 2 cut(s) 611, 1405
PinAI ACCGGT 2 cut(s) 116, 1058
PkrI GCNGC 7 cut(s) 82, 439, 578, 1239, 2296, 3525, 3558
PleI GAGTC 5 cut(s) 494, 1043, 1646, 2338, 2459
PluTI GGCGCC 1 cut(s) 3479
PmaCI CACGTG 1 cut(s) 2231
PmlI CACGTG 1 cut(s) 2231
PpsI GAGTC 5 cut(s) 494, 1043, 1646, 2338, 2459
Ppu21I YACGTR 1 cut(s) 2231
PpuMI RGGWCCY 2 cut(s) 784, 1758
PscI ACATGT 2 cut(s) 1669, 3531
PshAI GACNNNNGTC 2 cut(s) 966, 2646
PsiI TTATAA 1 cut(s) 1344
Psp5II RGGWCCY 2 cut(s) 784, 1758
Psp6I CCWGG 2 cut(s) 16, 2956
PspCI CACGTG 1 cut(s) 2231
PspFI CCCAGC 1 cut(s) 3471
PspGI CCWGG 2 cut(s) 16, 2956
PspLI CGTACG 1 cut(s) 3360
PspOMI GGGCCC 1 cut(s) 2107
PspPPI RGGWCCY 2 cut(s) 784, 1758
PstI CTGCAG 2 cut(s) 147, 578
PstNI CAGNNNCTG 2 cut(s) 2434, 2597
PsuI RGATCY 8 cut(s) 256, 748, 1155, 1216, 2756, 3157, 3339, 3481
PvuII CAGCTG 1 cut(s) 2639
RsaI GTAC 7 cut(s) 22, 180, 731, 861, 1644, 2764, 3362
RsaNI GTAC 7 cut(s) 21, 179, 730, 860, 1643, 2763, 3361
RseI CAYNNNNRTG 5 cut(s) 1827, 2655, 2919, 3378, 3536
SalI GTCGAC 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 857
SatI GCNGC 7 cut(s) 81, 438, 577, 1238, 2295, 3524, 3557
SchI GAGTC 5 cut(s) 494, 1043, 1646, 2339, 2459
ScrFI CCNGG 7 cut(s) 18, 741, 1196, 1603, 2508, 2958, 3290
SduI GDGCHC 4 cut(s) 884, 1115, 1446, 2111
SfaNI GCATC 7 cut(s) 431, 745, 1321, 1603, 2258, 2624, 2709
SfcI CTRYAG 6 cut(s) 110, 143, 574, 2187, 2610, 2976
SfoI GGCGCC 1 cut(s) 3477
SfuI TTCGAA 1 cut(s) 64
SinI GGWCC 5 cut(s) 784, 1249, 1599, 1680, 1758
SmiMI CAYNNNNRTG 5 cut(s) 1827, 2655, 2919, 3378, 3536
SmlI CTYRAG 1 cut(s) 1099
SmoI CTYRAG 1 cut(s) 1099
SpeI ACTAGT 2 cut(s) 3142, 3220
SphI GCATGC 1 cut(s) 1640
SseBI AGGCCT 1 cut(s) 3418
SsiI CCGC 4 cut(s) 350, 1334, 2179, 3556
SspDI GGCGCC 1 cut(s) 3475
SspI AATATT 4 cut(s) 103, 594, 1403, 3109
SspMI CTAG 7 cut(s) 347, 375, 818, 984, 1727, 3143, 3221
StuI AGGCCT 1 cut(s) 3418
StyD4I CCNGG 7 cut(s) 16, 739, 1194, 1601, 2506, 2956, 3288
StyI CCWWGG 2 cut(s) 411, 2113
TaiI ACGT 3 cut(s) 2233, 2541, 2969
TaqI TCGA 6 cut(s) 31, 64, 425, 894, 2736, 2863
TaqII GACCGA 1 cut(s) 1266
TatI WGTACW 2 cut(s) 178, 1642
TauI GCSGC 1 cut(s) 3559
TfiI GAWTC 6 cut(s) 290, 1511, 1528, 1792, 2896, 3440
TscAI CASTG 9 cut(s) 1113, 1710, 2020, 2439, 2475, 2569, 3380, 3417, 3473
TseFI GTSAC 8 cut(s) 68, 94, 1310, 2226, 2458, 2632, 3131, 3527
TseI GCWGC 6 cut(s) 80, 437, 576, 1237, 2294, 3523
Tsp45I GTSAC 8 cut(s) 68, 94, 1310, 2226, 2458, 2632, 3131, 3527
TspGWI ACGGA 4 cut(s) 658, 1910, 2477, 3240
TspRI CASTG 9 cut(s) 1113, 1710, 2020, 2439, 2475, 2569, 3380, 3417, 3473
Van91I CCANNNNNTGG 2 cut(s) 611, 1405
VneI GTGCAC 1 cut(s) 1442
VpaK11BI GGWCC 5 cut(s) 784, 1249, 1599, 1680, 1758
XapI RAATTY 9 cut(s) 125, 308, 566, 932, 1471, 1832, 1979, 2309, 2984
XceI RCATGY 5 cut(s) 1640, 1673, 3014, 3373, 3535
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XmiI GTMKAC 2 cut(s) 31, 109
XmnI GAANNNNTTC 4 cut(s) 289, 1557, 1671, 1791
XspI CTAG 7 cut(s) 347, 375, 818, 984, 1727, 3143, 3221
Zsp2I ATGCAT 1 cut(s) 1638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.