Prupe.1G061200_v2.0.a1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0012803
Physical Location & Seq
Forward (+)
4318313 .. 4324926
6614 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G061200.1

Sequence Viewer

Length: 3546 bp
ATGACTAGTGAGGCTCCTGGTACTTCTGGTCAACAGTTCTGCAAAGATACAATTAATCATGATCGAAGTAACAAAAGTGCTGCTGATGACAATGAAAATAATATTTGTGCACAGACTGGTGAGGAATTTTCAGCGGAGTTTCTGCAGGATCGTATTTCACAAAGAAGATTAGCCCCAGTAGTGACTGGCGTTGATCAGCGCCAGTCGAAAAGAGTGGGTTTTAATTTAAATAAAAACCATAAGCTTGTTTATGAGGATCTCGCTGGTGTTGTTGGGCTAAGGAGAATAGATTCTGACTGCAGTTCAGAATTCTCAGATTTTAGTCCAGCGGCTGCTACAGGCTTTGTGGCTGACATAGAGAAAAATGTTTATCCTAGTAACATAAGCAGATACCACTGGGAATATGGTGCTATTGGGCAAGTATCAGGTAAATTTTCGGATGAAGTTAATCGTGATCGGGTTATTGGAAAACCAACTACTCCACCTCTTTATGTACTGGAATCCCCTCAGTCATACCATCCTTGTGGTCAAGTATTTTCTGAAGGTTCTTTCTCTTTCAAAATGAAATTTCTCTGCAGCTTCGGGGGAAGAATATTACCAAGGCCAAATGATGGGAAGCTCAGATATGTGGGTGGAGATACACGGATTTTATCTATTCGCAAAGGCACCAATTTGGAGGAACTAATGAATAAGACTTATGCAATTTGTAACCAACCTCACACTATTAAGTACCAACTTCCTGGTGAGGATCTTGATGCACTTGTATCTGTGTGTTCAGATGAGGACCTTCATCATATGATAGAGGAATATCTTGAGCTAGAAAGAACTTCTCAAAGATTGCGGATCTTTCTTGTTCCTTTAAATGAAACTGAGAGCCCAAGTTCTGTGGAAGCAAGGGTAACGCATCCAATTGGTGCCGACTCCCAATTTGTTTTTGCTGTTAATGGCATGCTAGACCCAAGTCCTCGGAAAAGTTCCAGTGGGCAAAGTTTGGCCAGTCAGACTAGCCAGTTTGGAAATACCTCGGATTATAGTCCAACTTTTCGTAGAGAATCTCCTACAGCCACATATTTAGAGACCAAAGATTATAGCCCCAGTTCCTCAAATGTGGTGGGAACATTAACTAAACCTGCTCCTCAGTTCCTAGCTACTCTTCAGATCCCAAAGAAGTCTTTTAATCACTCCCCTCCCATATCTCCTGTTCCACTACAACATAGAGATCCCAAGAGTTCTAATGTGCAGTTTTATTTGGATCGACCATATTGTGATGGTAACGGAGGCATTGCTCCCTCTGTTATGGAAAAACTTCCATGTGACAATACCTATTATCTGGATGCTGTGGGCTATAATGAAAATCTTCATCATGGGCCTCCAGTGTTGAATTACCATCATCATAACAAATACTTGGCTAAAACTTCCCAGACAAGAAAGTCCCAGAATGTGCTCTCCCACAATCGTAGCTTTAGTGAGAATTCTGTGCCTTCTCCAAAGTATGGTCAAGGTGGAATGAACTCTGAGAGACTTGTGCCACTGGAAAAGGCCCTACATTCTGAAAAGTCTGTTTCTCATCCTACAGTGGGATTATTTTCAGGGTCTGATGATAGAGATGCTTCTGATCACAGAATAATGCATGCAGTACTTTCTGACTCTCAGTTGCAGGAACATGTTCAGAGGTCCTCAGAAGGAGAAGTTATCTCAGTGTCCTCATTGAAATGCAGAAGAGCAAAATTACCTTCACTGAAGATGCAAAGATCCTCGCAAGAATGGCCTGTGCAACAGGAAGACATGGTTGATGGGAAACATGAAATGGTTGAATATAGTAATCAATTCACAATCAGGAAACCTGACCAATGTAAGGAGGAACAAGGTCTGGGGATGTTGAACCGGACAGACAGGAATGACACTTGTGCTGATAAGAACTGGAACAACTTTGAAGGAAGTGTTGATGATATATCAAATGATACTGTCATGGAATTTAATAAGGTTCAAAATGTGAACTGCCTGAGCAGTGTTTCCTTATCTTCAGATGATTCACAATTCCCCGGAGGTGGGGTTTCTGGTTTACCAATTTCTGAAAATGAAGGTTCTAAAGATACTATGGGTCGCCAAGGCTACGAATTTGACACAACTTCTCAGTTGTTTCTTAGGAGCCAAAGTTGTACAAGGGATCAGCAGTGTGCTACAACTGAGAGAATAAGTGGTCAACCAGGGATCAGCAGGGTTGCCACTCAGGAATTCTTAGATAAATTGGCTACAAGTGCTGCTTCAGACGGAGAATATTCTCCCCTTGACAAGGACCCTGTGAATTATCCTGAGTATGTGGTCGAAAACGTAGGTCTTAGCAGACAGTCTTCTGAGGTAACCAAGTGTGACGATGCTATCCCTGTCCAGTCACAGTGCTTGGATAATCATCATGACAACAAGGCGACGGAATCAGTAGTTGTTGTTGAGGACTTAACTAATAGTACACCACCAGGTATCACCTCCTCTAAAGTGGCCTATCATGTCTCAAATATAGAAGATGAGGACAGTGATGAATGTTCGTCTCCTAGAGAAATAGACACAGGAAGTACAGCTCCAGAATCTGATGACAAGGGCGTGACAGCTGATGGCAATCATAGGCATGAAACTATTAGCGATGTTGCAATAGCTGAAATGGAAGCAGGCATCTATGGTTTGCAGATCATAAAGAATGATGATCTGGAAGAACTGCAAGAGTTAGGATCTGGTACATATGGAACTGTCTATCATGGGAAGTGGAGAGGAACAGATGTTGCTATTAAGAGAATAAAAAAGAGCTGTTTCTCCGGCAGATCATCAGAGCAAGAGCGACTGACTAAAGACTTCTGGAGAGAGGCAAAGATTCTGTCCACCCTTCACCATCCAAATGTTGTAGCATTTTATGGGGTGGTGCCTGATGGGCCTGGGGCAACATTGGCAACTGTAGCTGAATTTATGGTGAATGGATCACTGAGACATGTCCTAATAAGGAAGGATAGAGTGCTTGATCGTCGAAAGAGGCTCATAATCTTAATGGACGCAGCTTTTGGCATGGAGTATTTGCATTTGAAAAATATTGTCCACTTTGATTTGAAGTGTGACAATTTGCTTGTTAATCTGAGGGATCCTGAACGTCCCATATGCAAGGTTGGAGATTTTGGGTTGTCAAGAATCAAACGCAACACACTAGTATCTGGTGGCGTACGAGGAACCCTTCCGTGGATGGCACCAGAATTATTAAATGGAAGCAGTAACCGGGTCTCCGAGAAGGTTGATGTTTACTCATTTGGTATTGTAATGTGGGAGATCCTGACTGGGGAGGAACCGTATGCTAACATGCACTGCGGTGCTATCATAGGGGGAATTGTAAATAACACACTGAGGCCTCCTATTCCAAAGCGTTGTGATTCTGAGTGGAAAAATTTAATGGAACAGTGCTGGTCACCGGATCCTGCTGATCGCCCGTCATTCACAGAGATTACACACAGGCTGCGTGATATGTCAACGGCACTCCAGAAAAAGCGCCCTAATCTTGCAAGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1182

Amino Acids

131.31

Weight (kDa)

5.66

Isoelectric Point (pI)

51.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04700 AT1G04700 AT1G04700
fragaria_vesca FvH4_4g05890
malus_domestica MD13G1231300.v1.1 MD16G1235800.v1.1
prunus_persica Prupe.1G061200_v2.0.a1
pyrus_communis pycom13g20420
rosa_chinensis RchiOBHm_Chr4g0396991
rosa_laevigata RLG00000009461
rosa_multiflora Rmu_sc0002963.1_g000016
rosa_roxburghii Rroxscaffold_5G00341980
rosa_rugosa Rorug03G0346000
rosa_samantha Rh4AG075900 Rh4BG072300 Rh4CG080600 Rh4DG069700
rosa_wichuraiana Rw4G006090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1429
Acc36I ACCTGC 1 cut(s) 1138
AccB1I GGYRCC 4 cut(s) 665, 914, 2911, 3226
AccB7I CCANNNNNTGG 2 cut(s) 611, 1493
AciI CCGC 4 cut(s) 134, 329, 841, 3345
AcoI YGGCCR 1 cut(s) 993
AcuI CTGAAG 5 cut(s) 561, 1139, 1760, 2007, 2250
AfaI GTAC 9 cut(s) 22, 495, 731, 1638, 2161, 2467, 2572, 2731, 3204
AfiI CCNNNNNNNGG 9 cut(s) 611, 1493, 1577, 1855, 2048, 2049, 2293, 3219, 3316
AflIII ACRYGT 2 cut(s) 1663, 2977
AgsI TTSAA 9 cut(s) 559, 1381, 1711, 1814, 1882, 1934, 1988, 3070, 3094
AhlI ACTAGT 2 cut(s) 5, 3187
AjnI CCWGG 5 cut(s) 16, 739, 2206, 2473, 2923
AleI CACNNNNGTG 1 cut(s) 3345
Alw21I GWGCWC 2 cut(s) 112, 1446
Alw26I GTCTC 6 cut(s) 1070, 1513, 2512, 2550, 2968, 3265
Alw44I GTGCAC 1 cut(s) 108
AlwNI CAGNNNCTG 3 cut(s) 332, 1595, 2585
AoxI GGCC 8 cut(s) 602, 993, 1367, 1539, 1766, 2496, 2921, 3383
ApaLI GTGCAC 1 cut(s) 108
ApeKI GCWGC 6 cut(s) 80, 332, 576, 2261, 3041, 3490
ArsI GACNNNNNNTTYG 6 cut(s) 911, 943, 2852, 2884, 3029, 3061
AseI ATTAAT 1 cut(s) 54
Asp700I GAANNNNTTC 4 cut(s) 289, 1305, 1356, 1665
AspLEI GCGC 2 cut(s) 201, 3525
AspS9I GGNCC 6 cut(s) 784, 1367, 1540, 1674, 2296, 2921
AsuC2I CCSGG 2 cut(s) 2043, 3257
AsuHPI GGTGA 6 cut(s) 131, 755, 2473, 2870, 2971, 3435
AvaII GGWCC 3 cut(s) 784, 1674, 2296
BaeGI GKGCMC 1 cut(s) 112
BaeI ACNNNNGTAYC 2 cut(s) 3174, 3207
BalI TGGCCA 1 cut(s) 995
BamHI GGATCC 2 cut(s) 3124, 3448
BanI GGYRCC 4 cut(s) 665, 914, 2911, 3226
BanII GRGCYC 1 cut(s) 878
BbsI GAAGAC 2 cut(s) 1788, 2343
Bbv12I GWGCWC 2 cut(s) 112, 1446
BbvI GCAGC 6 cut(s) 67, 319, 588, 2248, 3053, 3477
BccI CCATC 9 cut(s) 525, 605, 1262, 1395, 1787, 2603, 2889, 2912, 3217
BceAI ACGGC 1 cut(s) 3522
BciT130I CCWGG 5 cut(s) 18, 741, 2208, 2475, 2925
BclI TGATCA 2 cut(s) 193, 1615
BcnI CCSGG 2 cut(s) 2043, 3257
BcoDI GTCTC 6 cut(s) 1070, 1513, 2512, 2550, 2968, 3265
BcuI ACTAGT 2 cut(s) 5, 3187
BfaI CTAG 8 cut(s) 6, 375, 818, 953, 1005, 1145, 2550, 3188
BfmI CTRYAG 7 cut(s) 143, 298, 336, 574, 1059, 1572, 2943
BfoI RGCGCY 2 cut(s) 202, 3526
BfuAI ACCTGC 1 cut(s) 1138
BglI GCCNNNNNGGC 1 cut(s) 2920
BisI GCNGC 7 cut(s) 81, 330, 333, 577, 2262, 3042, 3491
BlsI GCNGC 7 cut(s) 82, 331, 334, 578, 2263, 3043, 3492
BmcAI AGTACT 1 cut(s) 1638
Bme1390I CCNGG 7 cut(s) 18, 741, 2043, 2208, 2475, 2925, 3257
Bme18I GGWCC 3 cut(s) 784, 1674, 2296
BmgT120I GGNCC 6 cut(s) 784, 1367, 1540, 1674, 2296, 2921
BmrFI CCNGG 7 cut(s) 18, 741, 2043, 2208, 2475, 2925, 3257
BmrI ACTGGG 4 cut(s) 170, 406, 1089, 3324
BmsI GCATC 7 cut(s) 745, 913, 1324, 1597, 1734, 2365, 2676
BmuI ACTGGG 4 cut(s) 170, 406, 1089, 3324
BoxI GACNNNNGTC 1 cut(s) 960
BpiI GAAGAC 2 cut(s) 1788, 2343
BpmI CTGGAG 4 cut(s) 1356, 2562, 2869, 3497
Bpu10I CCTNAGC 2 cut(s) 278, 2003
BpuEI CTTGAG 1 cut(s) 833
BpuMI CCSGG 2 cut(s) 2043, 3257
BsaBI GATNNNNATC 2 cut(s) 2409, 2613
BsaI GGTCTC 2 cut(s) 1070, 3265
BsaJI CCNNGG 8 cut(s) 599, 965, 1023, 2041, 2107, 2207, 2924, 3218
BsaWI WCCGGW 2 cut(s) 1884, 3445
BsaXI ACNNNNNCTCC 8 cut(s) 2037, 2067, 2140, 2170, 3245, 3275, 3311, 3341
Bsc4I CCNNNNNNNGG 9 cut(s) 611, 1493, 1577, 1855, 2048, 2049, 2293, 3219, 3316
Bse3DI GCAATG 1 cut(s) 1281
Bse8I GATNNNNATC 2 cut(s) 2409, 2613
BseBI CCWGG 5 cut(s) 18, 741, 2208, 2475, 2925
BseDI CCNNGG 8 cut(s) 599, 965, 1023, 2041, 2107, 2207, 2924, 3218
BseGI GGATG 8 cut(s) 445, 517, 904, 1339, 1567, 1881, 2881, 3228
BseJI GATNNNNATC 2 cut(s) 2409, 2613
BseLI CCNNNNNNNGG 9 cut(s) 611, 1493, 1577, 1855, 2048, 2049, 2293, 3219, 3316
BseMI GCAATG 1 cut(s) 1281
BseRI GAGGAG 2 cut(s) 1125, 2476
BseSI GKGCMC 1 cut(s) 112
BseXI GCAGC 6 cut(s) 67, 319, 588, 2248, 3053, 3477
BsgI GTGCAG 1 cut(s) 1259
BshFI GGCC 8 cut(s) 604, 995, 1369, 1541, 1768, 2498, 2923, 3385
BshNI GGYRCC 4 cut(s) 665, 914, 2911, 3226
BsiHKAI GWGCWC 2 cut(s) 112, 1446
BsiSI CCGG 5 cut(s) 1885, 2043, 2808, 3256, 3446
BsiWI CGTACG 1 cut(s) 3202
BslFI GGGAC 2 cut(s) 1417, 3120
BslI CCNNNNNNNGG 9 cut(s) 611, 1493, 1577, 1855, 2048, 2049, 2293, 3219, 3316
BsmAI GTCTC 6 cut(s) 1070, 1513, 2512, 2550, 2968, 3265
BsmBI CGTCTC 1 cut(s) 2550
BsmFI GGGAC 2 cut(s) 1417, 3120
BsnI GGCC 8 cut(s) 604, 995, 1369, 1541, 1768, 2498, 2923, 3385
Bso31I GGTCTC 2 cut(s) 1070, 3265
Bsp1286I GDGCHC 3 cut(s) 112, 878, 1446
Bsp1407I TGTACA 1 cut(s) 2159
BspACI CCGC 4 cut(s) 134, 329, 841, 3345
BspANI GGCC 8 cut(s) 604, 995, 1369, 1541, 1768, 2498, 2923, 3385
BspHI TCATGA 2 cut(s) 58, 2413
BspMAI CTGCAG 3 cut(s) 147, 302, 578
BspMI ACCTGC 1 cut(s) 1138
BspQI GCTCTTC 1 cut(s) 1714
BspT107I GGYRCC 4 cut(s) 665, 914, 2911, 3226
BspTNI GGTCTC 2 cut(s) 1070, 3265
BsrDI GCAATG 1 cut(s) 1281
BsrGI TGTACA 1 cut(s) 2159
BssECI CCNNGG 8 cut(s) 599, 965, 1023, 2041, 2107, 2207, 2924, 3218
BssT1I CCWWGG 2 cut(s) 599, 2107
Bst2UI CCWGG 5 cut(s) 18, 741, 2208, 2475, 2925
Bst6I CTCTTC 2 cut(s) 1158, 1714
BstAUI TGTACA 1 cut(s) 2159
BstC8I GCNNGC 3 cut(s) 950, 1632, 2665
BstDSI CCRYGG 1 cut(s) 3218
BstEII GGTNACC 2 cut(s) 2359, 3441
BstENI CCTNNNNNAGG 1 cut(s) 2291
BstF5I GGATG 8 cut(s) 445, 517, 904, 1339, 1567, 1881, 2881, 3228
BstH2I RGCGCY 2 cut(s) 202, 3526
BstHHI GCGC 2 cut(s) 201, 3525
BstMAI GTCTC 6 cut(s) 1070, 1513, 2512, 2550, 2968, 3265
BstMWI GCNNNNNNNGC 5 cut(s) 1765, 2258, 2920, 2936, 2945
BstNI CCWGG 5 cut(s) 18, 741, 2208, 2475, 2925
BstNSI RCATGY 5 cut(s) 952, 1634, 1667, 2981, 3340
BstPAI GACNNNNGTC 1 cut(s) 960
BstPI GGTNACC 2 cut(s) 2359, 3441
BstSCI CCNGG 7 cut(s) 16, 739, 2041, 2206, 2473, 2923, 3255
BstSFI CTRYAG 7 cut(s) 143, 298, 336, 574, 1059, 1572, 2943
BstSLI GKGCMC 1 cut(s) 112
BstV1I GCAGC 6 cut(s) 67, 319, 588, 2248, 3053, 3477
BstV2I GAAGAC 2 cut(s) 1788, 2343
BstXI CCANNNNNNTGG 2 cut(s) 524, 740
BsuRI GGCC 8 cut(s) 604, 995, 1369, 1541, 1768, 2498, 2923, 3385
BtgI CCRYGG 1 cut(s) 3218
BtgZI GCGATG 1 cut(s) 2652
BtsCI GGATG 8 cut(s) 445, 517, 904, 1339, 1567, 1881, 2881, 3228
BtsI GCAGTG 3 cut(s) 2014, 2180, 3340
BveI ACCTGC 1 cut(s) 1138
Cac8I GCNNGC 3 cut(s) 950, 1632, 2665
CaiI CAGNNNCTG 3 cut(s) 332, 1595, 2585
CciI TCATGA 2 cut(s) 58, 2413
CfoI GCGC 2 cut(s) 201, 3525
Cfr13I GGNCC 6 cut(s) 784, 1367, 1540, 1674, 2296, 2921
CseI GACGC 1 cut(s) 3047
CsiI ACCWGGT 1 cut(s) 2473
Csp6I GTAC 9 cut(s) 21, 494, 730, 1637, 2160, 2466, 2571, 2730, 3203
CspCI CAANNNNNGTGG 4 cut(s) 867, 902, 1092, 1127
CviQI GTAC 9 cut(s) 21, 494, 730, 1637, 2160, 2466, 2571, 2730, 3203
DraI TTTAAA 2 cut(s) 228, 861
DrdI GACNNNNNNGTC 1 cut(s) 1429
DseDI GACNNNNNNGTC 1 cut(s) 1429
EaeI YGGCCR 1 cut(s) 993
Eam1104I CTCTTC 2 cut(s) 1158, 1714
EarI CTCTTC 2 cut(s) 1158, 1714
Eco130I CCWWGG 2 cut(s) 599, 2107
Eco147I AGGCCT 1 cut(s) 3385
Eco24I GRGCYC 1 cut(s) 878
Eco31I GGTCTC 2 cut(s) 1070, 3265
Eco47I GGWCC 3 cut(s) 784, 1674, 2296
Eco57I CTGAAG 5 cut(s) 561, 1139, 1760, 2007, 2250
Eco91I GGTNACC 2 cut(s) 2359, 3441
EcoNI CCTNNNNNAGG 1 cut(s) 2291
EcoO109I RGGNCCY 4 cut(s) 784, 1540, 1674, 2296
EcoO65I GGTNACC 2 cut(s) 2359, 3441
EcoRI GAATTC 3 cut(s) 308, 1471, 2234
EcoRII CCWGG 5 cut(s) 16, 739, 2206, 2473, 2923
EcoT14I CCWWGG 2 cut(s) 599, 2107
EcoT22I ATGCAT 1 cut(s) 1632
EcoT38I GRGCYC 1 cut(s) 878
ErhI CCWWGG 2 cut(s) 599, 2107
Esp3I CGTCTC 1 cut(s) 2550
FalI AAGNNNNNCTT 2 cut(s) 2248, 2280
FaqI GGGAC 2 cut(s) 1417, 3120
FauNDI CATATG 3 cut(s) 795, 2734, 3140
FbaI TGATCA 2 cut(s) 193, 1615
Fnu4HI GCNGC 7 cut(s) 81, 330, 333, 577, 2262, 3042, 3491
FokI GGATG 8 cut(s) 452, 504, 891, 1346, 1554, 1888, 2868, 3235
FriOI GRGCYC 1 cut(s) 878
Fsp4HI GCNGC 7 cut(s) 81, 330, 333, 577, 2262, 3042, 3491
FspBI CTAG 8 cut(s) 6, 375, 818, 953, 1005, 1145, 2550, 3188
GlaI GCGC 2 cut(s) 200, 3524
GluI GCNGC 7 cut(s) 81, 330, 333, 577, 2262, 3042, 3491
GsuI CTGGAG 4 cut(s) 1356, 2562, 2869, 3497
HaeII RGCGCY 2 cut(s) 202, 3526
HaeIII GGCC 8 cut(s) 604, 995, 1369, 1541, 1768, 2498, 2923, 3385
HapII CCGG 5 cut(s) 1885, 2043, 2808, 3256, 3446
HgaI GACGC 1 cut(s) 3047
HhaI GCGC 2 cut(s) 201, 3525
Hin6I GCGC 2 cut(s) 199, 3523
HinP1I GCGC 2 cut(s) 199, 3523
HincII GTYRAC 3 cut(s) 32, 2204, 3504
HindII GTYRAC 3 cut(s) 32, 2204, 3504
HindIII AAGCTT 1 cut(s) 242
HpaII CCGG 5 cut(s) 1885, 2043, 2808, 3256, 3446
HphI GGTGA 6 cut(s) 131, 755, 2473, 2870, 2971, 3435
Hpy99I CGWCG 2 cut(s) 2431, 3015
HpyCH4IV ACGT 2 cut(s) 2331, 3133
HpyF10VI GCNNNNNNNGC 5 cut(s) 1765, 2258, 2920, 2936, 2945
HpySE526I ACGT 2 cut(s) 2331, 3133
HspAI GCGC 2 cut(s) 199, 3523
Ksp22I TGATCA 2 cut(s) 193, 1615
LguI GCTCTTC 1 cut(s) 1714
LmnI GCTCC 5 cut(s) 19, 1138, 1291, 2148, 2581
Lsp1109I GCAGC 6 cut(s) 67, 319, 588, 2248, 3053, 3477
LweI GCATC 7 cut(s) 745, 913, 1324, 1597, 1734, 2365, 2676
MabI ACCWGGT 1 cut(s) 2473
MaeI CTAG 8 cut(s) 6, 375, 818, 953, 1005, 1145, 2550, 3188
MaeII ACGT 2 cut(s) 2331, 3133
MfeI CAATTG 1 cut(s) 909
MhlI GDGCHC 3 cut(s) 112, 878, 1446
MlsI TGGCCA 1 cut(s) 995
MluNI TGGCCA 1 cut(s) 995
MlyI GAGTC 2 cut(s) 914, 1640
MmeI TCCRAC 2 cut(s) 1061, 3130
Mox20I TGGCCA 1 cut(s) 995
Mph1103I ATGCAT 1 cut(s) 1632
MroXI GAANNNNTTC 4 cut(s) 289, 1305, 1356, 1665
MscI TGGCCA 1 cut(s) 995
MslI CAYNNNNRTG 5 cut(s) 522, 1711, 2622, 2886, 3345
Msp20I TGGCCA 1 cut(s) 995
MspA1I CMGCKG 3 cut(s) 134, 329, 2606
MspI CCGG 5 cut(s) 1885, 2043, 2808, 3256, 3446
MspR9I CCNGG 7 cut(s) 18, 741, 2043, 2208, 2475, 2925, 3257
MunI CAATTG 1 cut(s) 909
MvaI CCWGG 5 cut(s) 18, 741, 2208, 2475, 2925
MwoI GCNNNNNNNGC 5 cut(s) 1765, 2258, 2920, 2936, 2945
NciI CCSGG 2 cut(s) 2043, 3257
NdeI CATATG 3 cut(s) 795, 2734, 3140
NmuCI GTSAC 7 cut(s) 181, 1313, 2369, 2391, 2599, 3098, 3441
NsiI ATGCAT 1 cut(s) 1632
NspI RCATGY 5 cut(s) 952, 1634, 1667, 2981, 3340
OliI CACNNNNGTG 1 cut(s) 3345
PaeI GCATGC 2 cut(s) 952, 1634
PagI TCATGA 2 cut(s) 58, 2413
PceI AGGCCT 1 cut(s) 3385
PciI ACATGT 2 cut(s) 1663, 2977
PciSI GCTCTTC 1 cut(s) 1714
PdmI GAANNNNTTC 4 cut(s) 289, 1305, 1356, 1665
PfeI GAWTC 9 cut(s) 290, 500, 1052, 2030, 2432, 2582, 2863, 3171, 3407
Pfl23II CGTACG 1 cut(s) 3202
PflMI CCANNNNNTGG 2 cut(s) 611, 1493
PkrI GCNGC 7 cut(s) 82, 331, 334, 578, 2263, 3043, 3492
PleI GAGTC 2 cut(s) 914, 1640
PpsI GAGTC 2 cut(s) 914, 1640
PpuMI RGGWCCY 3 cut(s) 784, 1674, 2296
PscI ACATGT 2 cut(s) 1663, 2977
PshAI GACNNNNGTC 1 cut(s) 960
PshBI ATTAAT 1 cut(s) 54
Psp5II RGGWCCY 3 cut(s) 784, 1674, 2296
Psp6I CCWGG 5 cut(s) 16, 739, 2206, 2473, 2923
PspEI GGTNACC 2 cut(s) 2359, 3441
PspGI CCWGG 5 cut(s) 16, 739, 2206, 2473, 2923
PspLI CGTACG 1 cut(s) 3202
PspPI GGNCC 6 cut(s) 784, 1367, 1540, 1674, 2296, 2921
PspPPI RGGWCCY 3 cut(s) 784, 1674, 2296
PstI CTGCAG 3 cut(s) 147, 302, 578
PstNI CAGNNNCTG 3 cut(s) 332, 1595, 2585
PvuII CAGCTG 1 cut(s) 2606
RsaI GTAC 9 cut(s) 22, 495, 731, 1638, 2161, 2467, 2572, 2731, 3204
RsaNI GTAC 9 cut(s) 21, 494, 730, 1637, 2160, 2466, 2571, 2730, 3203
RseI CAYNNNNRTG 5 cut(s) 522, 1711, 2622, 2886, 3345
SapI GCTCTTC 1 cut(s) 1714
SatI GCNGC 7 cut(s) 81, 330, 333, 577, 2262, 3042, 3491
Sau96I GGNCC 6 cut(s) 784, 1367, 1540, 1674, 2296, 2921
ScaI AGTACT 1 cut(s) 1638
SchI GAGTC 2 cut(s) 914, 1640
ScrFI CCNGG 7 cut(s) 18, 741, 2043, 2208, 2475, 2925, 3257
SduI GDGCHC 3 cut(s) 112, 878, 1446
SexAI ACCWGGT 1 cut(s) 2473
SfaNI GCATC 7 cut(s) 745, 913, 1324, 1597, 1734, 2365, 2676
SfcI CTRYAG 7 cut(s) 143, 298, 336, 574, 1059, 1572, 2943
SinI GGWCC 3 cut(s) 784, 1674, 2296
SmiI ATTTAAAT 1 cut(s) 228
SmiMI CAYNNNNRTG 5 cut(s) 522, 1711, 2622, 2886, 3345
SmlI CTYRAG 1 cut(s) 812
SmoI CTYRAG 1 cut(s) 812
SpeI ACTAGT 2 cut(s) 5, 3187
SphI GCATGC 2 cut(s) 952, 1634
SseBI AGGCCT 1 cut(s) 3385
SsiI CCGC 4 cut(s) 134, 329, 841, 3345
SspI AATATT 4 cut(s) 103, 594, 2279, 3076
SspMI CTAG 8 cut(s) 6, 375, 818, 953, 1005, 1145, 2550, 3188
StuI AGGCCT 1 cut(s) 3385
StyD4I CCNGG 7 cut(s) 16, 739, 2041, 2206, 2473, 2923, 3255
StyI CCWWGG 2 cut(s) 599, 2107
SwaI ATTTAAAT 1 cut(s) 228
TaiI ACGT 2 cut(s) 2334, 3136
TaqI TCGA 5 cut(s) 64, 206, 1255, 2325, 3013
TatI WGTACW 5 cut(s) 493, 1636, 2159, 2465, 2570
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 9 cut(s) 290, 500, 1052, 2030, 2432, 2582, 2863, 3171, 3407
TseFI GTSAC 7 cut(s) 181, 1313, 2369, 2391, 2599, 3098, 3441
TseI GCWGC 6 cut(s) 80, 332, 576, 2261, 3041, 3490
Tsp45I GTSAC 7 cut(s) 181, 1313, 2369, 2391, 2599, 3098, 3441
TspGWI ACGGA 5 cut(s) 658, 1290, 2286, 2444, 3207
Van91I CCANNNNNTGG 2 cut(s) 611, 1493
VneI GTGCAC 1 cut(s) 108
VpaK11BI GGWCC 3 cut(s) 784, 1674, 2296
VspI ATTAAT 1 cut(s) 54
XagI CCTNNNNNAGG 1 cut(s) 2291
XceI RCATGY 5 cut(s) 952, 1634, 1667, 2981, 3340
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XmnI GAANNNNTTC 4 cut(s) 289, 1305, 1356, 1665
XspI CTAG 8 cut(s) 6, 375, 818, 953, 1005, 1145, 2550, 3188
ZrmI AGTACT 1 cut(s) 1638
Zsp2I ATGCAT 1 cut(s) 1632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.