MD13G1237500.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008156
Physical Location & Seq
Reverse (-)
24152964 .. 24156299
3336 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1237500.v1.1.491

Sequence Viewer

Length: 2409 bp
ATGTGGGCAAAGGGATTATTCACCTTCAAACTTTCAAAAGGCAGCCTCTGCCCTTCACTCGGACACTTTCATGCAATCTCCAAACTCTACAGTTCAAAATCCTGCACTTCATCCTCCAATTTCGTGATGGGTGAAAACCCAACTGCCATTGCCGCTGCTCTTTCCCTCTCTGAGAATTTGAAGTCAGCTTCTCTTGGTACCCAAATCCACTCCCGTGTCATCAAGTTGGGATTCACTAATGACTTTTTTTTATTCAATAATTTGATCAAAATGTACTCAAAATGTGGGGTTATGGATGATGCGTTCAAACTGTTTGATGAAATGCCTGAGAGAAACCTCGTCACTTGGACTTTGATGATTTCGGCTGCCGTCCAAGACGGTCAGTTTGAATTGGGCTTGGAGGTTTATTTGGAACTGGTAAGAAGTGGGTTGAGGCCGAATGAATTCACTGTTGGTAGCGTTCTCAAGGGGTGTGCAGCGTGTGCTAGTATCGAAGCATATGAATTCGGCATGAGTGTTCATTGTTTTGCATTGAAAACTGGGATTGAACAGAACTGCTATGTGGGTGGTTCTGTTTTGAATATGTATGCTAAGTTGGAGGATATTGAATCGGCGAAGGGGGTGTTTGAGTCTATAAGCAATCTTGATACTGCTGGTTGGAATGCTATGATTGGAGGTTACAGGCAATGTGGCTATGGATTTGAAGCATTGAAAGTTGTATGTTTGATGGTATGCAAAGGTATAAGCATGGACCAGTTCACCTTTGTTAATGCTCTAAAGGGTTGCTCTGTTGTCAGAAATTTGGATTTTGGGAAGCAACTTCACGGATTGATCATCCAAAGTGAGCTGAAATTAAGTACTTCAGTCATGAATGCTCTTATGGATATGTACTCTAGAAATGGTAGGATAGATTTGGCTTTGCAAGTTTTCAATAGGATACAAACGAAAGATGTCATATCGTGGAACACCGCATTTGGGGTCTTCTTTGAAGGCAAGAATGCAAGAGAAATTGCAAACTTAGTCCATGAGTTCATGCTAGAAAACATGAAGCCGAACCATATTACGTTCTCAATCTTGTTTAGGCAATGCAGTGAGCTACTTGATCTTAACCTTGGGCGTCAGTTTTTCTGCCTTGCATTACAATATGGGTTCTGGAATGAACCTAATGTCAGATGCTCAATAATCAATATGTTTTCTAGATGTGGGGCTATGGACATGGCACACTTGTTTTTTGGTGGCATACTTTACAAAAACATAACTTATTGGAATGAGTTGATTTCTGGGTATAATTCAAACTATTGTTATACAGAAGCCATGCAGATCTTTTGTAATTTATGGGACTTGGGAGTCGAAGCAAGTGAAGTCACGTTCTCCATCATACTGGAAGCTTGCTATAATGATGAACACCAATATATGACTAGACAAATTCATGGTGCAATTGTCAAGTCCGGCATCTCCTTCCATGGATATGTTTGTAGCTCATTAATTAAGTGCTATGTTAGATTTGGACTACTGGATGATTCCTTTGAGTTTCTTAAAGGGTTTGAGAGACTAGATTTGATATCTTGGGGAACTATGATATCTGCTCTGGTGCATCAGGGACATTTTTTTGAAGCTATAAAGATTTTCAACTCTCTGAGAGAAGTTGGTGGGAAGCCTGATGAATTTATTTTGGGAAGCATTCTAAATAGTTGTGCCGATACTGCAAGCTATCAGTTAGCTAAAGCTGTCCATTCCATTGTCATCAAAATGGGATTCCTCATGCAAGTCTTTGTTGTTAGTGCAGTTATAGATGCGTATGCAAAATGCGGGGATATTGGAAATTCAAGGATGACCTTTATCCAGTCACTTGGATCTGATGACGTGGTTATATATAATGCTATGATCATGGCTTGTGCTCATCATGGTCTGGCCGAGGAAGCGATGGATATCTTTGAGAAAATTCGGTTGGCAAATCTAAAGCCCAGCCAAGCCACATTTGTGTCTGCTATAGCAGCTTGTAGTCATGTGGGTTGGGTTGATCAAGGTCGTTCTTTGTTTGAGTTAATGAACTCAGATTACAAGATGGAACCAGTATCTCGGGATGTTTATGGTTGCATGGTTGATCTGCTTTCACGACACAGATACCTTGAAGATGCTAGACAAATGATTGAAGTGATGCCTTATACTCCTTGGCCTGCTGTATTGAGATCCTTGCTTAGCGGTTGTAGGATACATGGGAACACAGAGTTGGCAGAATGGACTGCTAAGAAGTTAGTTCAGTTGGTTTCAGAAAATGATGCACCTTATGTACTGTTGTCGAAGGTGTACTCTGAAGGAGGTAGTTGGGAAGGTGCTACAAAGGTAAGGAGGGAAATGATAGAAAGAGGTGTGCCAAAACATACAGGATATAGTTGGATCGAGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 Pkinase_Tyr

Protein Analysis

803

Amino Acids

89.75

Weight (kDa)

6.02

Isoelectric Point (pI)

30.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 83 - 112 1.2e-06 PPR repeat family
PPR PF01535 84 - 112 2.7e-06 PPR repeat
PPR_2 PF13041 112 - 159 1.7e-08 PPR repeat family
PPR_2 PF13041 216 - 262 2.1e-06 PPR repeat family
PPR_2 PF13041 621 - 668 5.6e-08 PPR repeat family
PPR PF01535 623 - 649 2.4e-06 PPR repeat
E_motif PF20431 739 - 801 3.2e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1346
Acc65I GGTACC 1 cut(s) 197
AccB1I GGYRCC 1 cut(s) 197
AciI CCGC 4 cut(s) 153, 969, 1809, 2202
AclWI GGATC 3 cut(s) 1861, 2184, 2405
AcoI YGGCCR 1 cut(s) 1911
AcsI RAATTY 8 cut(s) 175, 443, 503, 799, 1425, 1664, 1822, 1941
AcuI CTGAAG 2 cut(s) 846, 2334
AcyI GRCGYC 1 cut(s) 1117
AfaI GTAC 6 cut(s) 199, 275, 859, 890, 2292, 2309
AfiI CCNNNNNNNGG 2 cut(s) 59, 975
AjiI CACGTC 1 cut(s) 1864
AjuI GAANNNNNNNTTGG 6 cut(s) 435, 467, 1931, 1963, 2213, 2245
AleI CACNNNNGTG 2 cut(s) 213, 1979
AloI GAACNNNNNNTCC 2 cut(s) 287, 319
Alw21I GWGCWC 1 cut(s) 1900
Alw26I GTCTC 1 cut(s) 1543
AlwI GGATC 3 cut(s) 1861, 2184, 2405
AlwNI CAGNNNCTG 1 cut(s) 48
Ama87I CYCGRG 1 cut(s) 2079
AoxI GGCC 3 cut(s) 434, 1911, 2174
ApeKI GCWGC 5 cut(s) 42, 155, 365, 476, 1994
ApoI RAATTY 8 cut(s) 175, 443, 503, 799, 1425, 1664, 1822, 1941
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
AseI ATTAAT 1 cut(s) 1484
Asp700I GAANNNNTTC 1 cut(s) 443
Asp718I GGTACC 1 cut(s) 197
AspS9I GGNCC 1 cut(s) 751
AsuHPI GGTGA 3 cut(s) 13, 143, 751
AvaI CYCGRG 1 cut(s) 2079
AvaII GGWCC 1 cut(s) 751
BanI GGYRCC 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 973
Bbv12I GWGCWC 1 cut(s) 1900
BbvI GCAGC 5 cut(s) 54, 142, 352, 488, 2006
BccI CCATC 5 cut(s) 121, 721, 1382, 1918, 2059
BceAI ACGGC 1 cut(s) 353
BciVI GTATCC 2 cut(s) 930, 2205
BclI TGATCA 4 cut(s) 264, 831, 1884, 2020
BcoDI GTCTC 1 cut(s) 1543
BfaI CTAG 7 cut(s) 486, 894, 1037, 1197, 1419, 1553, 2139
BfmI CTRYAG 2 cut(s) 88, 1989
BfuI GTATCC 2 cut(s) 930, 2205
BglII AGATCT 1 cut(s) 1320
BisI GCNGC 6 cut(s) 43, 153, 156, 366, 477, 1995
BlpI GCTNAGC 1 cut(s) 2198
BlsI GCNGC 6 cut(s) 44, 154, 157, 367, 478, 1996
BmcAI AGTACT 1 cut(s) 859
Bme18I GGWCC 1 cut(s) 751
BmeT110I CYCGRG 1 cut(s) 2079
BmgBI CACGTC 1 cut(s) 1864
BmgT120I GGNCC 1 cut(s) 751
BmiI GGNNCC 2 cut(s) 199, 2070
BmrI ACTGGG 1 cut(s) 549
BmsI GCATC 8 cut(s) 289, 1163, 1461, 1603, 1783, 2125, 2148, 2269
BmuI ACTGGG 1 cut(s) 549
BpiI GAAGAC 1 cut(s) 973
Bpu1102I GCTNAGC 1 cut(s) 2198
BpuEI CTTGAG 1 cut(s) 449
BsaBI GATNNNNATC 1 cut(s) 1928
BsaHI GRCGYC 1 cut(s) 1117
BsaJI CCNNGG 4 cut(s) 1111, 1462, 1914, 2171
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 975
Bse1I ACTGG 7 cut(s) 420, 544, 754, 1386, 1518, 1843, 2072
Bse3DI GCAATG 3 cut(s) 147, 692, 1091
Bse8I GATNNNNATC 1 cut(s) 1928
BseDI CCNNGG 4 cut(s) 1111, 1462, 1914, 2171
BseGI GGATG 6 cut(s) 110, 301, 834, 1522, 1836, 2089
BseJI GATNNNNATC 1 cut(s) 1928
BseLI CCNNNNNNNGG 2 cut(s) 59, 975
BseMI GCAATG 3 cut(s) 147, 692, 1091
BseMII CTCAG 4 cut(s) 162, 318, 1628, 2067
BseNI ACTGG 7 cut(s) 420, 544, 754, 1386, 1518, 1843, 2072
BseXI GCAGC 5 cut(s) 54, 142, 352, 488, 2006
BseYI CCCAGC 1 cut(s) 1964
BsgI GTGCAG 3 cut(s) 88, 495, 1803
BshFI GGCC 3 cut(s) 436, 1913, 2176
BshNI GGYRCC 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 1900
BsiHKCI CYCGRG 1 cut(s) 2079
BsiSI CCGG 1 cut(s) 1449
BslFI GGGAC 2 cut(s) 1352, 1614
BslI CCNNNNNNNGG 2 cut(s) 59, 975
BsmAI GTCTC 1 cut(s) 1543
BsmFI GGGAC 2 cut(s) 1352, 1614
BsmI GAATGC 4 cut(s) 667, 877, 1003, 1680
BsnI GGCC 3 cut(s) 436, 1913, 2176
BsoBI CYCGRG 1 cut(s) 2079
Bsp1286I GDGCHC 1 cut(s) 1900
Bsp1720I GCTNAGC 1 cut(s) 2198
Bsp19I CCATGG 1 cut(s) 1462
BspACI CCGC 4 cut(s) 153, 969, 1809, 2202
BspANI GGCC 3 cut(s) 436, 1913, 2176
BspCNI CTCAG 4 cut(s) 163, 319, 1629, 2066
BspHI TCATGA 1 cut(s) 867
BspLI GGNNCC 2 cut(s) 199, 2070
BspPI GGATC 3 cut(s) 1861, 2184, 2405
BspT107I GGYRCC 1 cut(s) 197
BsrDI GCAATG 3 cut(s) 147, 692, 1091
BsrI ACTGG 7 cut(s) 420, 544, 754, 1386, 1518, 1843, 2072
BssECI CCNNGG 4 cut(s) 1111, 1462, 1914, 2171
BssNI GRCGYC 1 cut(s) 1117
BssT1I CCWWGG 3 cut(s) 1111, 1462, 2171
Bst4CI ACNGT 5 cut(s) 92, 312, 380, 451, 2295
BstACI GRCGYC 1 cut(s) 1117
BstAPI GCANNNNNTGC 2 cut(s) 48, 482
BstC8I GCNNGC 3 cut(s) 1390, 1708, 2178
BstDEI CTNAG 8 cut(s) 171, 327, 591, 1018, 1637, 2053, 2198, 2247
BstDSI CCRYGG 1 cut(s) 1462
BstF5I GGATG 6 cut(s) 110, 301, 834, 1522, 1836, 2089
BstMAI GTCTC 1 cut(s) 1543
BstMWI GCNNNNNNNGC 6 cut(s) 48, 152, 482, 1703, 1919, 1994
BstSFI CTRYAG 2 cut(s) 88, 1989
BstV1I GCAGC 5 cut(s) 54, 142, 352, 488, 2006
BstV2I GAAGAC 1 cut(s) 973
BstX2I RGATCY 3 cut(s) 1320, 1853, 2189
BstXI CCANNNNNNTGG 2 cut(s) 1381, 1850
BstYI RGATCY 3 cut(s) 1320, 1853, 2189
BsuI GTATCC 2 cut(s) 930, 2205
BsuRI GGCC 3 cut(s) 436, 1913, 2176
BtgI CCRYGG 1 cut(s) 1462
BtgZI GCGATG 1 cut(s) 1937
BtrI CACGTC 1 cut(s) 1864
BtsCI GGATG 6 cut(s) 110, 301, 834, 1522, 1836, 2089
BtsI GCAGTG 1 cut(s) 1096
BtsIMutI CAGTG 2 cut(s) 447, 1096
Cac8I GCNNGC 3 cut(s) 1390, 1708, 2178
CaiI CAGNNNCTG 1 cut(s) 48
CciI TCATGA 1 cut(s) 867
Cfr13I GGNCC 1 cut(s) 751
CseI GACGC 1 cut(s) 1106
Csp6I GTAC 6 cut(s) 198, 274, 858, 889, 2291, 2308
CviQI GTAC 6 cut(s) 198, 274, 858, 889, 2291, 2308
DdeI CTNAG 8 cut(s) 171, 327, 591, 1018, 1637, 2053, 2198, 2247
DrdI GACNNNNNNGTC 1 cut(s) 1346
DseDI GACNNNNNNGTC 1 cut(s) 1346
EaeI YGGCCR 1 cut(s) 1911
Eco130I CCWWGG 3 cut(s) 1111, 1462, 2171
Eco32I GATATC 3 cut(s) 1563, 1581, 1930
Eco47I GGWCC 1 cut(s) 751
Eco57I CTGAAG 2 cut(s) 846, 2334
Eco88I CYCGRG 1 cut(s) 2079
EcoRI GAATTC 2 cut(s) 443, 503
EcoRV GATATC 3 cut(s) 1563, 1581, 1930
EcoT14I CCWWGG 3 cut(s) 1111, 1462, 2171
ErhI CCWWGG 3 cut(s) 1111, 1462, 2171
FaqI GGGAC 2 cut(s) 1352, 1614
FauI CCCGC 1 cut(s) 1802
FauNDI CATATG 1 cut(s) 499
FbaI TGATCA 4 cut(s) 264, 831, 1884, 2020
Fnu4HI GCNGC 6 cut(s) 43, 153, 156, 366, 477, 1995
FokI GGATG 6 cut(s) 97, 308, 821, 1529, 1843, 2096
Fsp4HI GCNGC 6 cut(s) 43, 153, 156, 366, 477, 1995
FspBI CTAG 7 cut(s) 486, 894, 1037, 1197, 1419, 1553, 2139
GluI GCNGC 6 cut(s) 43, 153, 156, 366, 477, 1995
GsaI CCCAGC 1 cut(s) 1968
HaeIII GGCC 3 cut(s) 436, 1913, 2176
HapII CCGG 1 cut(s) 1449
HgaI GACGC 1 cut(s) 1106
Hin1I GRCGYC 1 cut(s) 1117
HindIII AAGCTT 1 cut(s) 1386
HinfI GANTC 6 cut(s) 231, 608, 629, 1347, 1520, 1755
HpaII CCGG 1 cut(s) 1449
HphI GGTGA 3 cut(s) 13, 143, 751
Hpy166II GTNNAC 2 cut(s) 759, 2308
Hpy188I TCNGA 9 cut(s) 62, 172, 797, 1172, 1638, 1858, 2056, 2272, 2314
Hpy188III TCNNGA 8 cut(s) 124, 644, 868, 894, 1153, 1197, 2081, 2115
Hpy8I GTNNAC 2 cut(s) 759, 2308
HpyAV CCTTC 8 cut(s) 34, 63, 610, 983, 1468, 2296, 2309, 2324
HpyCH4III ACNGT 5 cut(s) 92, 312, 380, 451, 2295
HpyCH4IV ACGT 3 cut(s) 1064, 1367, 1863
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 152, 482, 1703, 1919, 1994
HpyF3I CTNAG 8 cut(s) 171, 327, 591, 1018, 1637, 2053, 2198, 2247
HpySE526I ACGT 3 cut(s) 1064, 1367, 1863
Hsp92I GRCGYC 1 cut(s) 1117
KpnI GGTACC 1 cut(s) 201
Ksp22I TGATCA 4 cut(s) 264, 831, 1884, 2020
Lsp1109I GCAGC 5 cut(s) 54, 142, 352, 488, 2006
LweI GCATC 8 cut(s) 289, 1163, 1461, 1603, 1783, 2125, 2148, 2269
MaeI CTAG 7 cut(s) 486, 894, 1037, 1197, 1419, 1553, 2139
MaeII ACGT 3 cut(s) 1064, 1367, 1863
MaeIII GTNAC 4 cut(s) 340, 677, 1363, 1845
MboII GAAGA 2 cut(s) 973, 2144
MfeI CAATTG 1 cut(s) 1437
MflI RGATCY 3 cut(s) 1320, 1853, 2189
MhlI GDGCHC 1 cut(s) 1900
MlyI GAGTC 2 cut(s) 638, 1356
MmeI TCCRAC 3 cut(s) 576, 638, 2375
MroXI GAANNNNTTC 1 cut(s) 443
MseI TTAA 7 cut(s) 768, 854, 1107, 1484, 1488, 1536, 2045
MslI CAYNNNNRTG 5 cut(s) 69, 213, 1467, 1748, 1979
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 1 cut(s) 1449
MunI CAATTG 1 cut(s) 1437
Mva1269I GAATGC 4 cut(s) 667, 877, 1003, 1680
MwoI GCNNNNNNNGC 6 cut(s) 48, 152, 482, 1703, 1919, 1994
NcoI CCATGG 1 cut(s) 1462
NdeI CATATG 1 cut(s) 499
NlaIV GGNNCC 2 cut(s) 199, 2070
NmeAIII GCCGAG 1 cut(s) 1939
NmuCI GTSAC 3 cut(s) 340, 1363, 1845
OliI CACNNNNGTG 2 cut(s) 213, 1979
PacI TTAATTAA 1 cut(s) 1488
PagI TCATGA 1 cut(s) 867
PctI GAATGC 4 cut(s) 667, 877, 1003, 1680
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 4 cut(s) 231, 608, 1520, 1755
PkrI GCNGC 6 cut(s) 44, 154, 157, 367, 478, 1996
PleI GAGTC 2 cut(s) 637, 1355
PpsI GAGTC 2 cut(s) 637, 1355
PshBI ATTAAT 1 cut(s) 1484
PspFI CCCAGC 1 cut(s) 1964
PspN4I GGNNCC 2 cut(s) 199, 2070
PspPI GGNCC 1 cut(s) 751
PsrI GAACNNNNNNTAC 2 cut(s) 2042, 2074
PstNI CAGNNNCTG 1 cut(s) 48
PsuI RGATCY 3 cut(s) 1320, 1853, 2189
RsaI GTAC 6 cut(s) 199, 275, 859, 890, 2292, 2309
RsaNI GTAC 6 cut(s) 198, 274, 858, 889, 2291, 2308
RseI CAYNNNNRTG 5 cut(s) 69, 213, 1467, 1748, 1979
SaqAI TTAA 7 cut(s) 768, 854, 1107, 1484, 1488, 1536, 2045
SatI GCNGC 6 cut(s) 43, 153, 156, 366, 477, 1995
Sau96I GGNCC 1 cut(s) 751
ScaI AGTACT 1 cut(s) 859
SchI GAGTC 2 cut(s) 638, 1356
SduI GDGCHC 1 cut(s) 1900
SfaNI GCATC 8 cut(s) 289, 1163, 1461, 1603, 1783, 2125, 2148, 2269
SfcI CTRYAG 2 cut(s) 88, 1989
SinI GGWCC 1 cut(s) 751
SmiMI CAYNNNNRTG 5 cut(s) 69, 213, 1467, 1748, 1979
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
SsiI CCGC 4 cut(s) 153, 969, 1809, 2202
SspMI CTAG 7 cut(s) 486, 894, 1037, 1197, 1419, 1553, 2139
StyI CCWWGG 3 cut(s) 1111, 1462, 2171
TaaI ACNGT 5 cut(s) 92, 312, 380, 451, 2295
TaiI ACGT 3 cut(s) 1067, 1370, 1866
TaqI TCGA 4 cut(s) 492, 1350, 2300, 2400
TatI WGTACW 5 cut(s) 273, 857, 888, 2290, 2307
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 4 cut(s) 231, 608, 1520, 1755
Tru1I TTAA 7 cut(s) 768, 854, 1107, 1484, 1488, 1536, 2045
Tru9I TTAA 7 cut(s) 768, 854, 1107, 1484, 1488, 1536, 2045
TscAI CASTG 2 cut(s) 454, 1096
TseFI GTSAC 3 cut(s) 340, 1363, 1845
TseI GCWGC 5 cut(s) 42, 155, 365, 476, 1994
Tsp45I GTSAC 3 cut(s) 340, 1363, 1845
TspGWI ACGGA 1 cut(s) 840
TspRI CASTG 2 cut(s) 454, 1096
VpaK11BI GGWCC 1 cut(s) 751
VspI ATTAAT 1 cut(s) 1484
XapI RAATTY 8 cut(s) 175, 443, 503, 799, 1425, 1664, 1822, 1941
XbaI TCTAGA 2 cut(s) 893, 1196
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 7 cut(s) 486, 894, 1037, 1197, 1419, 1553, 2139
ZrmI AGTACT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.