pycom13g21030

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0008156
Physical Location & Seq
Reverse (-)
17252877 .. 17255285
2409 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g21030.1

Sequence Viewer

Length: 2409 bp
ATGTGGGCAAGGGGATTATTCACCTTCGAACTTTCAAAAGGCAGCCTCTGCCCTTCACTCAGGCACTTTCATGCGATCTCCAAACTCTACAGTTCAAAATCCTGCACTTCATCCTCCAATTTCGTGATGGGTGAAAACCCAACTGCCATTGCCGCTGCTCTTTCCCTCTCTGACAATTTGAAGTCAGCTTCTCTTGGTACCCAAATCCACTCCCGTGTCATCAAGTTGGGATTCACTAACGACGGTTTTGTATTCAATAATTTGATCAAAATGTACTCAAAATGTGGGATTATGGATGATGCGTTCAAACTGTTTGGTGAAATGCCTGAGAGAAACCTCGTCACTTGGACTTTGATGATTTCGGCTGCCGTCCAAGACGGTCAGTTTGAATTGGGCTTGGAGTTTTATTTGGAACTGGTAAGAAGTGGGTTGAGGCCGAATGAATTCACTGTTGGTAGTGTTCTCAAGGGGTGTGCAGCGTGTGCTAGTATCGAAGCACATGAATTCGGCATGAGTGTTCATTGTTTTGCATTGAAAACTGGGATCGAACAGAACTGCTATGTGGGTGGTTCTGTTTTGAACATGTATGCTAAGTTGGAGGATGTCGAATCGGCGAAGGGGGTGTTTGAGTCTATAAGCAATCTTGATACTGCCGGTTGGAATGCTATGATTGGAGGTTACAGGCAATGTGGCTATGGATTTGAAGCATTGAAAGTTGTATGTTTGATGGTATGCAAAGGTATAAGCATGGACCAGTTCACCTTTGTTAATGCTCTAAAGGGTTGCTCTGTTGTCGGAAATTTGGATTTTGGGAAGCAACTTCACGGATTGATCATCCAAAGTGAGCTGAAATTAAGTACTTCAGTCATGAATGCTCTTATGGATATGTACTCTAGAAATGGTAGGATGGATTTGGCTTTGCACGTTTTCAATAGGATACAAACGAAAGATGTCATATCGTGGAACACCGCATTTGGGGTCTTCTTCGAAGGCAAGAATGCAAGAGAAATTGCAAACTTAGTCCATGAGTTCATGCTAGAAAACATGAAGCCAAACCATATTACGTTCTCAGTCTTGTTTAGGCAATGCGGTGAGGTACTTGATCTTAACCTTGGGCGTCAGTTTTTCTGCCTTGCATTACAATATGGGTTCTGGAATGAACCTAATGTCAGAAGCTCAATAATCAGTATGTTTTCTAGATGTGGCGCTATGGACATGGCACACTTGTTTTTTGATGGCATACTTTACAAAAACATAACTTATTGGAATGAGTTGATTTCTGGGTATAATTCAAACTATTGTTATTCAGAAGCCATGCAGATCTTTTGTAATTTATGGGACTTGGGAGTTGAAGCGAGTGAAGTCACATTCTCCATCATACTGGAAGCTTGCTATAATGATGAACACCAATATATGACTAGACAAATTCATGGTGCAGTTGTCAAGTCGGGCATCTCCTTCCACGGATATGTTTGTAGCTCATTAATTAAGTGCTATGTTAGATTTGGACTACTGGATGATTCCTTTGAGTTTCTTAAAGGGTTTGAGAGACTAGATTTGGTATCTTGGGGAACTATGATATCTGCTCTGGTGCATCAGGGACATTTTTTTGAAGCTATAAAGATTTTCAACTCTCTGAGAGAAGTTGGTGGGAAGCCTGATGAATTTATATTGGGAAGCATTCTAAATAGTTGTGCCGATATTGCAAGCTATCAGTTAGTTAAATCTGTCCATTCCATTGTCATCAAAATGGGATTCCACATGCAAGTCTTTGTTGTTAGTGCAGTTATAGATGCGTATGCAAAATGCGGGGATATTGGAAATTCAAGGATGACCTTTATCCAGTCACTTGGATCTGATGACGTGGTTATATATAATGCTATGATCATGGCTTGCGCTCATCATGGTCTGGCCGAGGAAGCGATGGATATCTTTGAGAAAATTCGGTTGGCAAATCTAAAGCCCAGCCAAGCCACATTTGTGTCTGCTATAGCTGCTTGTAGTCATGTGGGTTGGGTTGATCAAGGTCGTTCTTTGTTTGAGTTAATGAACTCTGATTACAAGATGGAACCAGTATCTCAGGATGTTTATGGTTGCATGGTTGATCTGCTTTCACGATACGGATACCTTGAAGATGCTAGACAAATGATTGAAGTGATGCCTTATACTCCTTGGCCTGCTATATTGAGATCCTTGCTTAGCGGTTGTAGGATACATGGGAACAGAGAGTTGGGAGAATGGACTGCTAAGAAGTTAGTTCAGTTGGTTCCAGAAAATGATGTACCTTATGTACTGTTGTCGAAGGTGTACTCTGAAGGAGGTAGTTGGGAAGGTGCTACAAAGATATGGAGGGAAATGATAGAAAGAGGTGTGCCAAAAAATAAAGGATATAGTTGGATCGAGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 Pkinase_Tyr

Protein Analysis

803

Amino Acids

89.69

Weight (kDa)

5.87

Isoelectric Point (pI)

30.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 84 - 112 9.6e-06 PPR repeat
PPR_2 PF13041 112 - 159 2.1e-08 PPR repeat family
PPR_2 PF13041 216 - 262 2.1e-06 PPR repeat family
PPR_2 PF13041 621 - 668 5.6e-08 PPR repeat family
PPR PF01535 623 - 649 2.4e-06 PPR repeat
E_motif PF20431 739 - 801 1.3e-11 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 197
AccB1I GGYRCC 1 cut(s) 197
AciI CCGC 5 cut(s) 153, 969, 1089, 1809, 2202
AclWI GGATC 4 cut(s) 551, 1861, 2184, 2405
AcoI YGGCCR 1 cut(s) 1911
AcsI RAATTY 7 cut(s) 443, 503, 799, 1425, 1664, 1822, 1941
AcuI CTGAAG 2 cut(s) 846, 2334
AcyI GRCGYC 1 cut(s) 1117
AfaI GTAC 8 cut(s) 199, 275, 859, 890, 1098, 2283, 2292, 2309
AfiI CCNNNNNNNGG 1 cut(s) 975
AflIII ACRYGT 1 cut(s) 582
AjiI CACGTC 1 cut(s) 1864
AjuI GAANNNNNNNTTGG 6 cut(s) 435, 467, 1931, 1963, 2213, 2245
AleI CACNNNNGTG 2 cut(s) 213, 1979
AloI GAACNNNNNNTCC 2 cut(s) 287, 319
AluBI AGCT 8 cut(s) 188, 847, 1176, 1388, 1479, 1616, 1710, 1994
AluI AGCT 8 cut(s) 188, 847, 1176, 1388, 1479, 1616, 1710, 1994
Alw26I GTCTC 1 cut(s) 1543
AlwI GGATC 4 cut(s) 551, 1861, 2184, 2405
AlwNI CAGNNNCTG 1 cut(s) 48
AoxI GGCC 3 cut(s) 434, 1911, 2174
ApeKI GCWGC 5 cut(s) 42, 155, 365, 476, 1994
ApoI RAATTY 7 cut(s) 443, 503, 799, 1425, 1664, 1822, 1941
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
AseI ATTAAT 1 cut(s) 1484
Asp700I GAANNNNTTC 1 cut(s) 443
Asp718I GGTACC 1 cut(s) 197
AspLEI GCGC 2 cut(s) 1208, 1898
AspS9I GGNCC 1 cut(s) 751
AsuHPI GGTGA 5 cut(s) 13, 143, 329, 751, 1103
AsuII TTCGAA 2 cut(s) 27, 987
AvaII GGWCC 1 cut(s) 751
BanI GGYRCC 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 973
BbvI GCAGC 5 cut(s) 54, 142, 352, 488, 1981
BccI CCATC 7 cut(s) 121, 721, 901, 1229, 1382, 1918, 2059
BceAI ACGGC 1 cut(s) 353
BciVI GTATCC 3 cut(s) 930, 2117, 2205
BclI TGATCA 4 cut(s) 264, 831, 1884, 2020
BcoDI GTCTC 1 cut(s) 1543
BfaI CTAG 7 cut(s) 486, 894, 1037, 1197, 1419, 1553, 2139
BfmI CTRYAG 2 cut(s) 88, 1989
BfoI RGCGCY 1 cut(s) 1209
BfuI GTATCC 3 cut(s) 930, 2117, 2205
BglII AGATCT 1 cut(s) 1320
BisI GCNGC 6 cut(s) 43, 153, 156, 366, 477, 1995
BlpI GCTNAGC 1 cut(s) 2198
BlsI GCNGC 6 cut(s) 44, 154, 157, 367, 478, 1996
BmcAI AGTACT 1 cut(s) 859
Bme18I GGWCC 1 cut(s) 751
BmgBI CACGTC 1 cut(s) 1864
BmgT120I GGNCC 1 cut(s) 751
BmiI GGNNCC 3 cut(s) 199, 2070, 2268
BmrI ACTGGG 1 cut(s) 549
BmsI GCATC 6 cut(s) 289, 1461, 1603, 1783, 2125, 2148
BmuI ACTGGG 1 cut(s) 549
BpiI GAAGAC 1 cut(s) 973
Bpu1102I GCTNAGC 1 cut(s) 2198
Bpu14I TTCGAA 2 cut(s) 27, 987
BpuEI CTTGAG 1 cut(s) 449
BsaBI GATNNNNATC 1 cut(s) 1928
BsaHI GRCGYC 1 cut(s) 1117
BsaJI CCNNGG 4 cut(s) 1111, 1462, 1914, 2171
Bsc4I CCNNNNNNNGG 1 cut(s) 975
Bse118I RCCGGY 1 cut(s) 653
Bse1I ACTGG 7 cut(s) 420, 544, 754, 1386, 1518, 1843, 2072
Bse3DI GCAATG 3 cut(s) 147, 692, 1091
Bse8I GATNNNNATC 1 cut(s) 1928
BseDI CCNNGG 4 cut(s) 1111, 1462, 1914, 2171
BseGI GGATG 8 cut(s) 110, 301, 607, 834, 912, 1522, 1836, 2089
BseJI GATNNNNATC 1 cut(s) 1928
BseLI CCNNNNNNNGG 1 cut(s) 975
BseMI GCAATG 3 cut(s) 147, 692, 1091
BseMII CTCAG 5 cut(s) 73, 318, 1083, 1628, 2093
BseNI ACTGG 7 cut(s) 420, 544, 754, 1386, 1518, 1843, 2072
BseXI GCAGC 5 cut(s) 54, 142, 352, 488, 1981
BseYI CCCAGC 1 cut(s) 1964
BsgI GTGCAG 4 cut(s) 88, 495, 1455, 1803
BshFI GGCC 3 cut(s) 436, 1913, 2176
BshNI GGYRCC 1 cut(s) 197
BsiSI CCGG 1 cut(s) 654
BslFI GGGAC 2 cut(s) 1352, 1614
BslI CCNNNNNNNGG 1 cut(s) 975
BsmAI GTCTC 1 cut(s) 1543
BsmFI GGGAC 2 cut(s) 1352, 1614
BsmI GAATGC 4 cut(s) 667, 877, 1003, 1680
BsnI GGCC 3 cut(s) 436, 1913, 2176
Bsp119I TTCGAA 2 cut(s) 27, 987
Bsp1720I GCTNAGC 1 cut(s) 2198
BspACI CCGC 5 cut(s) 153, 969, 1089, 1809, 2202
BspANI GGCC 3 cut(s) 436, 1913, 2176
BspCNI CTCAG 5 cut(s) 72, 319, 1082, 1629, 2092
BspHI TCATGA 1 cut(s) 867
BspLI GGNNCC 3 cut(s) 199, 2070, 2268
BspPI GGATC 4 cut(s) 551, 1861, 2184, 2405
BspT104I TTCGAA 2 cut(s) 27, 987
BspT107I GGYRCC 1 cut(s) 197
BsrDI GCAATG 3 cut(s) 147, 692, 1091
BsrFI RCCGGY 1 cut(s) 653
BsrI ACTGG 7 cut(s) 420, 544, 754, 1386, 1518, 1843, 2072
BssAI RCCGGY 1 cut(s) 653
BssECI CCNNGG 4 cut(s) 1111, 1462, 1914, 2171
BssNI GRCGYC 1 cut(s) 1117
BssT1I CCWWGG 2 cut(s) 1111, 2171
Bst4CI ACNGT 6 cut(s) 92, 245, 312, 380, 451, 2295
BstACI GRCGYC 1 cut(s) 1117
BstAPI GCANNNNNTGC 2 cut(s) 48, 482
BstBI TTCGAA 2 cut(s) 27, 987
BstC8I GCNNGC 4 cut(s) 1390, 1708, 1894, 2178
BstDEI CTNAG 9 cut(s) 59, 327, 591, 1018, 1069, 1637, 2079, 2198, 2247
BstDSI CCRYGG 1 cut(s) 1462
BstF5I GGATG 8 cut(s) 110, 301, 607, 834, 912, 1522, 1836, 2089
BstH2I RGCGCY 1 cut(s) 1209
BstHHI GCGC 2 cut(s) 1208, 1898
BstMAI GTCTC 1 cut(s) 1543
BstMWI GCNNNNNNNGC 6 cut(s) 48, 152, 482, 1703, 1919, 1994
BstNSI RCATGY 2 cut(s) 586, 1765
BstSFI CTRYAG 2 cut(s) 88, 1989
BstV1I GCAGC 5 cut(s) 54, 142, 352, 488, 1981
BstV2I GAAGAC 1 cut(s) 973
BstX2I RGATCY 3 cut(s) 1320, 1853, 2189
BstXI CCANNNNNNTGG 2 cut(s) 1381, 1850
BstYI RGATCY 3 cut(s) 1320, 1853, 2189
BsuI GTATCC 3 cut(s) 930, 2117, 2205
BsuRI GGCC 3 cut(s) 436, 1913, 2176
BtgI CCRYGG 1 cut(s) 1462
BtgZI GCGATG 1 cut(s) 1937
BtrI CACGTC 1 cut(s) 1864
BtsCI GGATG 8 cut(s) 110, 301, 607, 834, 912, 1522, 1836, 2089
BtsIMutI CAGTG 1 cut(s) 447
Cac8I GCNNGC 4 cut(s) 1390, 1708, 1894, 2178
CaiI CAGNNNCTG 1 cut(s) 48
CciI TCATGA 1 cut(s) 867
CfoI GCGC 2 cut(s) 1208, 1898
Cfr10I RCCGGY 1 cut(s) 653
Cfr13I GGNCC 1 cut(s) 751
CseI GACGC 1 cut(s) 1106
Csp6I GTAC 8 cut(s) 198, 274, 858, 889, 1097, 2282, 2291, 2308
CviQI GTAC 8 cut(s) 198, 274, 858, 889, 1097, 2282, 2291, 2308
DdeI CTNAG 9 cut(s) 59, 327, 591, 1018, 1069, 1637, 2079, 2198, 2247
EaeI YGGCCR 1 cut(s) 1911
Eco130I CCWWGG 2 cut(s) 1111, 2171
Eco32I GATATC 2 cut(s) 1581, 1930
Eco47I GGWCC 1 cut(s) 751
Eco57I CTGAAG 2 cut(s) 846, 2334
EcoRI GAATTC 2 cut(s) 443, 503
EcoRV GATATC 2 cut(s) 1581, 1930
EcoT14I CCWWGG 2 cut(s) 1111, 2171
ErhI CCWWGG 2 cut(s) 1111, 2171
FaqI GGGAC 2 cut(s) 1352, 1614
FauI CCCGC 1 cut(s) 1802
FbaI TGATCA 4 cut(s) 264, 831, 1884, 2020
Fnu4HI GCNGC 6 cut(s) 43, 153, 156, 366, 477, 1995
FokI GGATG 8 cut(s) 97, 308, 614, 821, 919, 1529, 1843, 2096
Fsp4HI GCNGC 6 cut(s) 43, 153, 156, 366, 477, 1995
FspBI CTAG 7 cut(s) 486, 894, 1037, 1197, 1419, 1553, 2139
GlaI GCGC 2 cut(s) 1207, 1897
GluI GCNGC 6 cut(s) 43, 153, 156, 366, 477, 1995
GsaI CCCAGC 1 cut(s) 1968
HaeII RGCGCY 1 cut(s) 1209
HaeIII GGCC 3 cut(s) 436, 1913, 2176
HapII CCGG 1 cut(s) 654
HgaI GACGC 1 cut(s) 1106
HhaI GCGC 2 cut(s) 1208, 1898
Hin1I GRCGYC 1 cut(s) 1117
Hin6I GCGC 2 cut(s) 1206, 1896
HinP1I GCGC 2 cut(s) 1206, 1896
HindIII AAGCTT 1 cut(s) 1386
HinfI GANTC 5 cut(s) 231, 608, 629, 1520, 1755
HpaII CCGG 1 cut(s) 654
HphI GGTGA 5 cut(s) 13, 143, 329, 751, 1103
Hpy166II GTNNAC 2 cut(s) 759, 2308
Hpy188I TCNGA 8 cut(s) 172, 797, 1172, 1309, 1638, 1858, 2056, 2314
Hpy188III TCNNGA 9 cut(s) 124, 644, 868, 894, 1153, 1197, 2081, 2115, 2270
Hpy8I GTNNAC 2 cut(s) 759, 2308
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 8 cut(s) 34, 63, 610, 983, 1468, 2296, 2309, 2324
HpyCH4III ACNGT 6 cut(s) 92, 245, 312, 380, 451, 2295
HpyCH4IV ACGT 3 cut(s) 924, 1064, 1863
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 152, 482, 1703, 1919, 1994
HpyF3I CTNAG 9 cut(s) 59, 327, 591, 1018, 1069, 1637, 2079, 2198, 2247
HpySE526I ACGT 3 cut(s) 924, 1064, 1863
Hsp92I GRCGYC 1 cut(s) 1117
HspAI GCGC 2 cut(s) 1206, 1896
KpnI GGTACC 1 cut(s) 201
Ksp22I TGATCA 4 cut(s) 264, 831, 1884, 2020
Lsp1109I GCAGC 5 cut(s) 54, 142, 352, 488, 1981
LweI GCATC 6 cut(s) 289, 1461, 1603, 1783, 2125, 2148
MaeI CTAG 7 cut(s) 486, 894, 1037, 1197, 1419, 1553, 2139
MaeII ACGT 3 cut(s) 924, 1064, 1863
MaeIII GTNAC 4 cut(s) 340, 677, 1363, 1845
MboII GAAGA 3 cut(s) 973, 976, 2144
MflI RGATCY 3 cut(s) 1320, 1853, 2189
MlyI GAGTC 1 cut(s) 638
MmeI TCCRAC 4 cut(s) 576, 638, 775, 2375
MroXI GAANNNNTTC 1 cut(s) 443
MseI TTAA 8 cut(s) 768, 854, 1107, 1484, 1488, 1536, 1722, 2045
MslI CAYNNNNRTG 5 cut(s) 69, 213, 1467, 1748, 1979
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 1 cut(s) 654
Mva1269I GAATGC 4 cut(s) 667, 877, 1003, 1680
MwoI GCNNNNNNNGC 6 cut(s) 48, 152, 482, 1703, 1919, 1994
NlaIV GGNNCC 3 cut(s) 199, 2070, 2268
NmeAIII GCCGAG 1 cut(s) 1939
NmuCI GTSAC 3 cut(s) 340, 1363, 1845
NspI RCATGY 2 cut(s) 586, 1765
NspV TTCGAA 2 cut(s) 27, 987
OliI CACNNNNGTG 2 cut(s) 213, 1979
PacI TTAATTAA 1 cut(s) 1488
PagI TCATGA 1 cut(s) 867
PciI ACATGT 1 cut(s) 582
PctI GAATGC 4 cut(s) 667, 877, 1003, 1680
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 4 cut(s) 231, 608, 1520, 1755
PkrI GCNGC 6 cut(s) 44, 154, 157, 367, 478, 1996
PleI GAGTC 1 cut(s) 637
PpsI GAGTC 1 cut(s) 637
PscI ACATGT 1 cut(s) 582
PshBI ATTAAT 1 cut(s) 1484
PspFI CCCAGC 1 cut(s) 1964
PspN4I GGNNCC 3 cut(s) 199, 2070, 2268
PspPI GGNCC 1 cut(s) 751
PsrI GAACNNNNNNTAC 2 cut(s) 2042, 2074
PstNI CAGNNNCTG 1 cut(s) 48
PsuI RGATCY 3 cut(s) 1320, 1853, 2189
RsaI GTAC 8 cut(s) 199, 275, 859, 890, 1098, 2283, 2292, 2309
RsaNI GTAC 8 cut(s) 198, 274, 858, 889, 1097, 2282, 2291, 2308
RseI CAYNNNNRTG 5 cut(s) 69, 213, 1467, 1748, 1979
SaqAI TTAA 8 cut(s) 768, 854, 1107, 1484, 1488, 1536, 1722, 2045
SatI GCNGC 6 cut(s) 43, 153, 156, 366, 477, 1995
Sau96I GGNCC 1 cut(s) 751
ScaI AGTACT 1 cut(s) 859
SchI GAGTC 1 cut(s) 638
SfaNI GCATC 6 cut(s) 289, 1461, 1603, 1783, 2125, 2148
SfcI CTRYAG 2 cut(s) 88, 1989
SfuI TTCGAA 2 cut(s) 27, 987
SinI GGWCC 1 cut(s) 751
SmiMI CAYNNNNRTG 5 cut(s) 69, 213, 1467, 1748, 1979
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
SsiI CCGC 5 cut(s) 153, 969, 1089, 1809, 2202
SspMI CTAG 7 cut(s) 486, 894, 1037, 1197, 1419, 1553, 2139
StyI CCWWGG 2 cut(s) 1111, 2171
TaaI ACNGT 6 cut(s) 92, 245, 312, 380, 451, 2295
TaiI ACGT 3 cut(s) 927, 1067, 1866
TaqI TCGA 7 cut(s) 27, 492, 546, 606, 987, 2300, 2400
TatI WGTACW 5 cut(s) 273, 857, 888, 2290, 2307
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 4 cut(s) 231, 608, 1520, 1755
Tru1I TTAA 8 cut(s) 768, 854, 1107, 1484, 1488, 1536, 1722, 2045
Tru9I TTAA 8 cut(s) 768, 854, 1107, 1484, 1488, 1536, 1722, 2045
TscAI CASTG 1 cut(s) 454
TseFI GTSAC 3 cut(s) 340, 1363, 1845
TseI GCWGC 5 cut(s) 42, 155, 365, 476, 1994
Tsp45I GTSAC 3 cut(s) 340, 1363, 1845
TspGWI ACGGA 3 cut(s) 840, 1479, 2136
TspRI CASTG 1 cut(s) 454
VpaK11BI GGWCC 1 cut(s) 751
VspI ATTAAT 1 cut(s) 1484
XapI RAATTY 7 cut(s) 443, 503, 799, 1425, 1664, 1822, 1941
XbaI TCTAGA 2 cut(s) 893, 1196
XceI RCATGY 2 cut(s) 586, 1765
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 7 cut(s) 486, 894, 1037, 1197, 1419, 1553, 2139
ZrmI AGTACT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.