MD13G1249500.v1.1

Wiskott-Aldrich syndrome protein family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016073
Physical Location & Seq
Forward (+)
26505725 .. 26509037
3313 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1249500.v1.1.491

Sequence Viewer

Length: 417 bp
ATGGCCATCAACAGGGACTCCACTCCCCCTCCAATGATCGGCAAGATTGGGCCCTACACTGTCTTTATGACGCCGCCCGAACCGGTTTTTGAATCTCCCCGGAAGGTGGCCGCGCCGGTCTTTGAGTCCCCCAAGAAGGTGGCCCCGCCCCCGGTCCAGCCGCCGCCTCAGCAATTGCTGAAGCCCATTGCCGTATCTTCGGATTCCTCGTCCGATGGCTCGGTGTTGGGGATTTTGAAGAATGCGCTCACCAAAGTGCAGAAGGCTCATTCAAGTTTGGACGACCATTTGGCACGCTGGTTTGGATTAAATCAGTCAAAATATCAGTGGGCGCTAGATGATTATTACCAGAGCAAGGGAGTGGAAAAGGACGATGCAAAACCAAAAGAGATATCTAGCAAAATGCAGAGTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAM177

Protein Analysis

139

Amino Acids

15.13

Weight (kDa)

9.1

Isoelectric Point (pI)

61.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18910
fragaria_vesca FvH4_4g07900
malus_domestica MD13G1249500.v1.1 MD16G1255800.v1.1
prunus_persica Prupe.1G082700_v2.0.a1
pyrus_communis pycom13g21990 pycom16g21700
rosa_chinensis RchiOBHm_Chr4g0401201
rosa_laevigata RLG00000009156
rosa_rugosa Rorug04G0028900
rosa_samantha Rh4AG106400 Rh4BG101100 Rh4CG115400
rosa_wichuraiana Rw4G008610 Rw4G008640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 113
AciI CCGC 5 cut(s) 74, 111, 146, 161, 164
AcoI YGGCCR 2 cut(s) 3, 108
AcuI CTGAAG 1 cut(s) 200
AcyI GRCGYC 1 cut(s) 71
AfiI CCNNNNNNNGG 6 cut(s) 12, 38, 106, 136, 151, 355
AgeI ACCGGT 1 cut(s) 82
AgsI TTSAA 3 cut(s) 92, 238, 273
AleI CACNNNNGTG 1 cut(s) 254
AoxI GGCC 4 cut(s) 3, 50, 108, 141
ApaI GGGCCC 1 cut(s) 54
AsiGI ACCGGT 1 cut(s) 82
AspLEI GCGC 3 cut(s) 115, 247, 334
AspS9I GGNCC 4 cut(s) 50, 51, 142, 154
AsuC2I CCSGG 2 cut(s) 100, 152
AsuHPI GGTGA 1 cut(s) 241
AvaII GGWCC 1 cut(s) 154
BaeGI GKGCMC 1 cut(s) 54
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 54
BbvCI CCTCAGC 1 cut(s) 168
BccI CCATC 2 cut(s) 14, 209
BceAI ACGGC 1 cut(s) 176
BcnI CCSGG 2 cut(s) 100, 152
BfaI CTAG 2 cut(s) 335, 396
BfoI RGCGCY 1 cut(s) 335
BisI GCNGC 4 cut(s) 74, 111, 161, 164
BlsI GCNGC 4 cut(s) 75, 112, 162, 165
Bme1390I CCNGG 2 cut(s) 100, 152
Bme18I GGWCC 1 cut(s) 154
BmgT120I GGNCC 4 cut(s) 50, 51, 142, 154
BmiI GGNNCC 2 cut(s) 52, 144
BmrFI CCNGG 2 cut(s) 100, 152
BmsI GCATC 1 cut(s) 364
Bpu10I CCTNAGC 1 cut(s) 168
BpuMI CCSGG 2 cut(s) 100, 152
BsaHI GRCGYC 1 cut(s) 71
BsaJI CCNNGG 2 cut(s) 98, 150
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 3 cut(s) 13, 32, 43
Bsc4I CCNNNNNNNGG 6 cut(s) 12, 38, 106, 136, 151, 355
Bse118I RCCGGY 2 cut(s) 82, 115
Bse3DI GCAATG 1 cut(s) 186
BseDI CCNNGG 2 cut(s) 98, 150
BseLI CCNNNNNNNGG 6 cut(s) 12, 38, 106, 136, 151, 355
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 1 cut(s) 182
BseSI GKGCMC 1 cut(s) 54
BsgI GTGCAG 1 cut(s) 278
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 4 cut(s) 5, 52, 110, 143
BshTI ACCGGT 1 cut(s) 82
BsiSI CCGG 4 cut(s) 83, 100, 116, 152
BslFI GGGAC 2 cut(s) 29, 112
BslI CCNNNNNNNGG 6 cut(s) 12, 38, 106, 136, 151, 355
BsmFI GGGAC 2 cut(s) 29, 112
BsmI GAATGC 1 cut(s) 247
BsnI GGCC 4 cut(s) 5, 52, 110, 143
Bsp120I GGGCCC 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 54
Bsp143I GATC 1 cut(s) 36
BspACI CCGC 5 cut(s) 74, 111, 146, 161, 164
BspANI GGCC 4 cut(s) 5, 52, 110, 143
BspCNI CTCAG 1 cut(s) 181
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 2 cut(s) 52, 144
BsrDI GCAATG 1 cut(s) 186
BsrFI RCCGGY 2 cut(s) 82, 115
BssAI RCCGGY 2 cut(s) 82, 115
BssECI CCNNGG 2 cut(s) 98, 150
BssMI GATC 1 cut(s) 36
BssNI GRCGYC 1 cut(s) 71
Bst4CI ACNGT 1 cut(s) 61
BstACI GRCGYC 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 295
BstDEI CTNAG 1 cut(s) 168
BstFNI CGCG 1 cut(s) 113
BstH2I RGCGCY 1 cut(s) 335
BstHHI GCGC 3 cut(s) 115, 247, 334
BstKTI GATC 1 cut(s) 39
BstMBI GATC 1 cut(s) 36
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstSCI CCNGG 2 cut(s) 98, 150
BstSLI GKGCMC 1 cut(s) 54
BstUI CGCG 1 cut(s) 113
BstXI CCANNNNNNTGG 1 cut(s) 139
BsuRI GGCC 4 cut(s) 5, 52, 110, 143
BtsIMutI CAGTG 2 cut(s) 57, 332
Cac8I GCNNGC 1 cut(s) 295
CfoI GCGC 3 cut(s) 115, 247, 334
Cfr10I RCCGGY 2 cut(s) 82, 115
Cfr13I GGNCC 4 cut(s) 50, 51, 142, 154
CseI GACGC 1 cut(s) 79
CspAI ACCGGT 1 cut(s) 82
CviJI RGCY 8 cut(s) 5, 52, 110, 143, 160, 184, 219, 266
CviKI_1 RGCY 8 cut(s) 5, 52, 110, 143, 160, 184, 219, 266
DdeI CTNAG 1 cut(s) 168
DpnI GATC 1 cut(s) 38
DpnII GATC 1 cut(s) 36
EaeI YGGCCR 2 cut(s) 3, 108
Eco24I GRGCYC 1 cut(s) 54
Eco32I GATATC 1 cut(s) 393
Eco47I GGWCC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 51
EcoRV GATATC 1 cut(s) 393
EcoT38I GRGCYC 1 cut(s) 54
FaiI YATR 1 cut(s) 68
FaqI GGGAC 2 cut(s) 29, 112
FauI CCCGC 1 cut(s) 153
Fnu4HI GCNGC 4 cut(s) 74, 111, 161, 164
FriOI GRGCYC 1 cut(s) 54
Fsp4HI GCNGC 4 cut(s) 74, 111, 161, 164
FspBI CTAG 2 cut(s) 335, 396
GlaI GCGC 3 cut(s) 114, 246, 333
GluI GCNGC 4 cut(s) 74, 111, 161, 164
HaeII RGCGCY 1 cut(s) 335
HaeIII GGCC 4 cut(s) 5, 52, 110, 143
HapII CCGG 4 cut(s) 83, 100, 116, 152
HgaI GACGC 1 cut(s) 79
HhaI GCGC 3 cut(s) 115, 247, 334
Hin1I GRCGYC 1 cut(s) 71
Hin6I GCGC 3 cut(s) 113, 245, 332
HinP1I GCGC 3 cut(s) 113, 245, 332
HinfI GANTC 4 cut(s) 17, 92, 125, 203
HpaII CCGG 4 cut(s) 83, 100, 116, 152
HphI GGTGA 1 cut(s) 241
Hpy188I TCNGA 2 cut(s) 202, 214
HpyAV CCTTC 3 cut(s) 97, 130, 256
HpyCH4III ACNGT 1 cut(s) 61
HpyCH4V TGCA 3 cut(s) 259, 377, 406
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
HpyF3I CTNAG 1 cut(s) 168
Hsp92I GRCGYC 1 cut(s) 71
HspAI GCGC 3 cut(s) 113, 245, 332
Kzo9I GATC 1 cut(s) 36
LpnPI CCDG 7 cut(s) 96, 113, 129, 165, 170, 283, 362
LweI GCATC 1 cut(s) 364
MaeI CTAG 2 cut(s) 335, 396
MalI GATC 1 cut(s) 38
MboI GATC 1 cut(s) 36
MboII GAAGA 2 cut(s) 189, 250
MfeI CAATTG 1 cut(s) 173
MhlI GDGCHC 1 cut(s) 54
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 173
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 11, 134
MnlI CCTC 3 cut(s) 39, 177, 217
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 308
MslI CAYNNNNRTG 1 cut(s) 254
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 83, 100, 116, 152
MspR9I CCNGG 2 cut(s) 100, 152
MunI CAATTG 1 cut(s) 173
Mva1269I GAATGC 1 cut(s) 247
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 169
NciI CCSGG 2 cut(s) 100, 152
NdeII GATC 1 cut(s) 36
NlaIV GGNNCC 2 cut(s) 52, 144
OliI CACNNNNGTG 1 cut(s) 254
PcsI WCGNNNNNNNCGW 1 cut(s) 206
PctI GAATGC 1 cut(s) 247
PfeI GAWTC 2 cut(s) 92, 203
PinAI ACCGGT 1 cut(s) 82
PkrI GCNGC 4 cut(s) 75, 112, 162, 165
PleI GAGTC 2 cut(s) 11, 133
PpsI GAGTC 2 cut(s) 11, 133
PspN4I GGNNCC 2 cut(s) 52, 144
PspOMI GGGCCC 1 cut(s) 50
PspPI GGNCC 4 cut(s) 50, 51, 142, 154
RseI CAYNNNNRTG 1 cut(s) 254
SaqAI TTAA 1 cut(s) 308
SatI GCNGC 4 cut(s) 74, 111, 161, 164
Sau3AI GATC 1 cut(s) 36
Sau96I GGNCC 4 cut(s) 50, 51, 142, 154
SchI GAGTC 2 cut(s) 11, 134
ScrFI CCNGG 2 cut(s) 100, 152
SduI GDGCHC 1 cut(s) 54
SetI ASST 2 cut(s) 108, 141
SfaNI GCATC 1 cut(s) 364
SinI GGWCC 1 cut(s) 154
SmiMI CAYNNNNRTG 1 cut(s) 254
Sse9I AATT 1 cut(s) 173
SsiI CCGC 5 cut(s) 74, 111, 146, 161, 164
SspMI CTAG 2 cut(s) 335, 396
StyD4I CCNGG 2 cut(s) 98, 150
TaaI ACNGT 1 cut(s) 61
TasI AATT 1 cut(s) 173
TauI GCSGC 4 cut(s) 76, 113, 163, 166
TfiI GAWTC 2 cut(s) 92, 203
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TscAI CASTG 2 cut(s) 64, 332
TspRI CASTG 2 cut(s) 64, 332
VpaK11BI GGWCC 1 cut(s) 154
XspI CTAG 2 cut(s) 335, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.