MD16G1255800.v1.1

Wiskott-Aldrich syndrome protein family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016073
Physical Location & Seq
Forward (+)
29445417 .. 29448763
3347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1255800.v1.1.491

Sequence Viewer

Length: 435 bp
ATGGCCATCAACAGGGACTCCACTCCCCCTCCAATGATCGGCAAGATTGGGCCCTACACTGTGTTCATGACTCCGCCCTCCACCCCTAAGCCGCCTGAACCGGTCTTTGAATCTCCCCGGAAGGTGGCCGCGCCGGTCTTTGAGTCCCCCAAGAAGGTGGCCCCGCCTCCGGTTCAGCCGCCGCCTCAGCAATTGGTGAAGCCCATTGCCGTATCATCGGATTCCTCGGCCGATGGGTCGGTGTTGGGTATTTTTAAGAATGCTGTCACCAAAGTGCAGAAGGCTCATTCAAGTTTGGACGACCATTTGGCACGTTGGTTTGGGTTAAATCAGTCAAAATATCAATGGGCACTAGATGATTATTACCAGAGCAAGGGGGTGGAAAAGGACGGTGCAAAACCAAAAGAGATATCTAGCAAAATGCAGACTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAM177

Protein Analysis

145

Amino Acids

15.68

Weight (kDa)

9.43

Isoelectric Point (pI)

54.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18910
fragaria_vesca FvH4_4g07900
malus_domestica MD13G1249500.v1.1 MD16G1255800.v1.1
prunus_persica Prupe.1G082700_v2.0.a1
pyrus_communis pycom13g21990 pycom16g21700
rosa_chinensis RchiOBHm_Chr4g0401201
rosa_laevigata RLG00000009156
rosa_rugosa Rorug04G0028900
rosa_samantha Rh4AG106400 Rh4BG101100 Rh4CG115400
rosa_wichuraiana Rw4G008610 Rw4G008640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 131
AciI CCGC 6 cut(s) 74, 92, 129, 164, 179, 182
AcoI YGGCCR 3 cut(s) 3, 126, 228
AfiI CCNNNNNNNGG 6 cut(s) 12, 38, 124, 154, 169, 373
AgeI ACCGGT 1 cut(s) 100
AgsI TTSAA 2 cut(s) 110, 291
AleI CACNNNNGTG 1 cut(s) 272
AoxI GGCC 5 cut(s) 3, 50, 126, 159, 228
ApaI GGGCCC 1 cut(s) 54
AsiGI ACCGGT 1 cut(s) 100
AspLEI GCGC 1 cut(s) 133
AspS9I GGNCC 3 cut(s) 50, 51, 160
AsuC2I CCSGG 1 cut(s) 118
AsuHPI GGTGA 2 cut(s) 208, 259
BaeGI GKGCMC 2 cut(s) 54, 352
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 54
BbvCI CCTCAGC 1 cut(s) 186
BccI CCATC 2 cut(s) 14, 227
BceAI ACGGC 1 cut(s) 194
BcnI CCSGG 1 cut(s) 118
BfaI CTAG 2 cut(s) 353, 414
BisI GCNGC 4 cut(s) 92, 129, 179, 182
BlsI GCNGC 4 cut(s) 93, 130, 180, 183
Bme1390I CCNGG 1 cut(s) 118
BmgT120I GGNCC 3 cut(s) 50, 51, 160
BmiI GGNNCC 2 cut(s) 52, 162
BmrFI CCNGG 1 cut(s) 118
Bpu10I CCTNAGC 2 cut(s) 87, 186
BpuMI CCSGG 1 cut(s) 118
BsaJI CCNNGG 2 cut(s) 116, 225
BsaWI WCCGGW 2 cut(s) 100, 169
BsaXI ACNNNNNCTCC 3 cut(s) 13, 32, 43
Bsc4I CCNNNNNNNGG 6 cut(s) 12, 38, 124, 154, 169, 373
Bse118I RCCGGY 2 cut(s) 100, 133
Bse3DI GCAATG 1 cut(s) 204
BseDI CCNNGG 2 cut(s) 116, 225
BseLI CCNNNNNNNGG 6 cut(s) 12, 38, 124, 154, 169, 373
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 1 cut(s) 200
BseSI GKGCMC 2 cut(s) 54, 352
BseX3I CGGCCG 1 cut(s) 228
BsgI GTGCAG 1 cut(s) 296
Bsh1236I CGCG 1 cut(s) 131
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 5 cut(s) 5, 52, 128, 161, 230
BshTI ACCGGT 1 cut(s) 100
BsiEI CGRYCG 1 cut(s) 231
BsiSI CCGG 4 cut(s) 101, 118, 134, 170
BslFI GGGAC 2 cut(s) 29, 130
BslI CCNNNNNNNGG 6 cut(s) 12, 38, 124, 154, 169, 373
BsmFI GGGAC 2 cut(s) 29, 130
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 5 cut(s) 5, 52, 128, 161, 230
Bsp120I GGGCCC 1 cut(s) 50
Bsp1286I GDGCHC 2 cut(s) 54, 352
Bsp143I GATC 1 cut(s) 36
BspACI CCGC 6 cut(s) 74, 92, 129, 164, 179, 182
BspANI GGCC 5 cut(s) 5, 52, 128, 161, 230
BspCNI CTCAG 1 cut(s) 199
BspFNI CGCG 1 cut(s) 131
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 2 cut(s) 52, 162
BsrDI GCAATG 1 cut(s) 204
BsrFI RCCGGY 2 cut(s) 100, 133
BssAI RCCGGY 2 cut(s) 100, 133
BssECI CCNNGG 2 cut(s) 116, 225
BssMI GATC 1 cut(s) 36
Bst4CI ACNGT 3 cut(s) 61, 392, 430
BstDEI CTNAG 2 cut(s) 87, 186
BstFNI CGCG 1 cut(s) 131
BstHHI GCGC 1 cut(s) 133
BstKTI GATC 1 cut(s) 39
BstMBI GATC 1 cut(s) 36
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 1 cut(s) 187
BstSCI CCNGG 1 cut(s) 116
BstSLI GKGCMC 2 cut(s) 54, 352
BstUI CGCG 1 cut(s) 131
BstXI CCANNNNNNTGG 1 cut(s) 157
BstZI CGGCCG 1 cut(s) 228
BsuRI GGCC 5 cut(s) 5, 52, 128, 161, 230
BtsIMutI CAGTG 1 cut(s) 57
CciI TCATGA 1 cut(s) 66
CfoI GCGC 1 cut(s) 133
Cfr10I RCCGGY 2 cut(s) 100, 133
Cfr13I GGNCC 3 cut(s) 50, 51, 160
CspAI ACCGGT 1 cut(s) 100
CviAII CATG 1 cut(s) 67
CviJI RGCY 9 cut(s) 5, 52, 91, 128, 161, 178, 202, 230, 284
CviKI_1 RGCY 9 cut(s) 5, 52, 91, 128, 161, 178, 202, 230, 284
DdeI CTNAG 2 cut(s) 87, 186
DpnI GATC 1 cut(s) 38
DpnII GATC 1 cut(s) 36
EaeI YGGCCR 3 cut(s) 3, 126, 228
EagI CGGCCG 1 cut(s) 228
EciI GGCGGA 1 cut(s) 63
EclXI CGGCCG 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 54
Eco32I GATATC 1 cut(s) 411
Eco52I CGGCCG 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 51
EcoRV GATATC 1 cut(s) 411
EcoT38I GRGCYC 1 cut(s) 54
FaeI CATG 1 cut(s) 70
FaiI YATR 1 cut(s) 68
FaqI GGGAC 2 cut(s) 29, 130
FatI CATG 1 cut(s) 66
FauI CCCGC 1 cut(s) 171
Fnu4HI GCNGC 4 cut(s) 92, 129, 179, 182
FriOI GRGCYC 1 cut(s) 54
Fsp4HI GCNGC 4 cut(s) 92, 129, 179, 182
FspBI CTAG 2 cut(s) 353, 414
GlaI GCGC 1 cut(s) 132
GluI GCNGC 4 cut(s) 92, 129, 179, 182
HaeIII GGCC 5 cut(s) 5, 52, 128, 161, 230
HapII CCGG 4 cut(s) 101, 118, 134, 170
HhaI GCGC 1 cut(s) 133
Hin1II CATG 1 cut(s) 70
Hin6I GCGC 1 cut(s) 131
HinP1I GCGC 1 cut(s) 131
HinfI GANTC 5 cut(s) 17, 70, 110, 143, 221
HpaII CCGG 4 cut(s) 101, 118, 134, 170
HphI GGTGA 2 cut(s) 208, 259
Hpy188I TCNGA 1 cut(s) 220
Hpy188III TCNNGA 1 cut(s) 67
HpyAV CCTTC 3 cut(s) 115, 148, 274
HpyCH4III ACNGT 3 cut(s) 61, 392, 430
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 3 cut(s) 277, 395, 424
HpyF10VI GCNNNNNNNGC 1 cut(s) 187
HpyF3I CTNAG 2 cut(s) 87, 186
HpySE526I ACGT 1 cut(s) 313
Hsp92II CATG 1 cut(s) 70
HspAI GCGC 1 cut(s) 131
Kzo9I GATC 1 cut(s) 36
LpnPI CCDG 6 cut(s) 108, 114, 131, 147, 183, 380
MaeI CTAG 2 cut(s) 353, 414
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 1 cut(s) 265
MalI GATC 1 cut(s) 38
MboI GATC 1 cut(s) 36
MfeI CAATTG 1 cut(s) 191
MhlI GDGCHC 2 cut(s) 54, 352
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 191
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 11, 64, 152
MnlI CCTC 5 cut(s) 39, 88, 177, 195, 235
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 255, 326
MslI CAYNNNNRTG 1 cut(s) 272
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 101, 118, 134, 170
MspR9I CCNGG 1 cut(s) 118
MunI CAATTG 1 cut(s) 191
Mva1269I GAATGC 1 cut(s) 265
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 1 cut(s) 187
NciI CCSGG 1 cut(s) 118
NdeII GATC 1 cut(s) 36
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 2 cut(s) 52, 162
NmeAIII GCCGAG 1 cut(s) 206
NmuCI GTSAC 1 cut(s) 265
OliI CACNNNNGTG 1 cut(s) 272
PagI TCATGA 1 cut(s) 66
PctI GAATGC 1 cut(s) 265
PfeI GAWTC 2 cut(s) 110, 221
PinAI ACCGGT 1 cut(s) 100
PkrI GCNGC 4 cut(s) 93, 130, 180, 183
PleI GAGTC 3 cut(s) 11, 64, 151
PpsI GAGTC 3 cut(s) 11, 64, 151
PspN4I GGNNCC 2 cut(s) 52, 162
PspOMI GGGCCC 1 cut(s) 50
PspPI GGNCC 3 cut(s) 50, 51, 160
RseI CAYNNNNRTG 1 cut(s) 272
SaqAI TTAA 2 cut(s) 255, 326
SatI GCNGC 4 cut(s) 92, 129, 179, 182
Sau3AI GATC 1 cut(s) 36
Sau96I GGNCC 3 cut(s) 50, 51, 160
SchI GAGTC 3 cut(s) 11, 64, 152
ScrFI CCNGG 1 cut(s) 118
SduI GDGCHC 2 cut(s) 54, 352
SetI ASST 3 cut(s) 126, 159, 316
SmiMI CAYNNNNRTG 1 cut(s) 272
Sse9I AATT 1 cut(s) 191
SsiI CCGC 6 cut(s) 74, 92, 129, 164, 179, 182
SspMI CTAG 2 cut(s) 353, 414
StyD4I CCNGG 1 cut(s) 116
TaaI ACNGT 3 cut(s) 61, 392, 430
TaiI ACGT 1 cut(s) 316
TasI AATT 1 cut(s) 191
TauI GCSGC 4 cut(s) 94, 131, 181, 184
TfiI GAWTC 2 cut(s) 110, 221
Tru1I TTAA 2 cut(s) 255, 326
Tru9I TTAA 2 cut(s) 255, 326
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 1 cut(s) 265
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 1 cut(s) 55
TspRI CASTG 1 cut(s) 64
XspI CTAG 2 cut(s) 353, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.