MD13G1257300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005201
Physical Location & Seq
Reverse (-)
28949882 .. 28952038
2157 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1257300.v1.1.491

Sequence Viewer

Length: 303 bp
ATGTGTGGTGCTAGGGAGTTGTTGGATGAACTCCAGGTTCAGGAATCACAAAGGTGTGGTGACTACGAGGAATTCGACGGTGTTCTGACAGATTGGACAAAATTAGGACTCCCTTCGGGTGTATCAACTTATAAATTGTATCTTAAAGCTTCCGTACTGTGCAAATGGTTACGTCACTCTCACGTGACGGCCAGCATGCCCTCCTTCGGGACGGGGTGTGTCAGTTGGTATCAGAGCATGGTTGCAATCTTGGACATTTTGAGAAGTTCTAGTGAATTCTGTCAGAACTACACCGCCTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

101

Amino Acids

11.23

Weight (kDa)

5.19

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 132
AciI CCGC 1 cut(s) 294
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 2 cut(s) 71, 275
AcvI CACGTG 1 cut(s) 184
AfaI GTAC 1 cut(s) 156
AfiI CCNNNNNNNGG 3 cut(s) 40, 206, 207
AjnI CCWGG 1 cut(s) 33
AleI CACNNNNGTG 1 cut(s) 52
AluBI AGCT 1 cut(s) 149
AluI AGCT 1 cut(s) 149
AoxI GGCC 1 cut(s) 189
ApoI RAATTY 2 cut(s) 71, 275
AsuHPI GGTGA 1 cut(s) 71
BbrPI CACGTG 1 cut(s) 184
BceAI ACGGC 1 cut(s) 204
BciT130I CCWGG 1 cut(s) 35
BfaI CTAG 2 cut(s) 12, 270
Bme1390I CCNGG 1 cut(s) 35
BmrFI CCNGG 1 cut(s) 35
BpmI CTGGAG 1 cut(s) 17
BsaAI YACGTR 1 cut(s) 184
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 206, 207
BseBI CCWGG 1 cut(s) 35
BseGI GGATG 1 cut(s) 31
BseLI CCNNNNNNNGG 3 cut(s) 40, 206, 207
BshFI GGCC 1 cut(s) 191
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 3 cut(s) 40, 206, 207
BsmFI GGGAC 1 cut(s) 223
BsnI GGCC 1 cut(s) 191
BspACI CCGC 1 cut(s) 294
BspANI GGCC 1 cut(s) 191
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 2 cut(s) 80, 159
BstBAI YACGTR 1 cut(s) 184
BstC8I GCNNGC 2 cut(s) 193, 197
BstF5I GGATG 1 cut(s) 31
BstNI CCWGG 1 cut(s) 35
BstNSI RCATGY 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 33
BsuRI GGCC 1 cut(s) 191
BtsCI GGATG 1 cut(s) 31
Cac8I GCNNGC 2 cut(s) 193, 197
Csp6I GTAC 1 cut(s) 155
CviAII CATG 2 cut(s) 196, 238
CviJI RGCY 2 cut(s) 149, 191
CviKI_1 RGCY 2 cut(s) 149, 191
CviQI GTAC 1 cut(s) 155
EaeI YGGCCR 1 cut(s) 189
Eco72I CACGTG 1 cut(s) 184
EcoRI GAATTC 2 cut(s) 71, 275
EcoRII CCWGG 1 cut(s) 33
FaeI CATG 2 cut(s) 199, 241
FaiI YATR 3 cut(s) 132, 197, 239
FaqI GGGAC 1 cut(s) 223
FatI CATG 2 cut(s) 195, 237
FokI GGATG 1 cut(s) 38
FspBI CTAG 2 cut(s) 12, 270
GsuI CTGGAG 1 cut(s) 17
HaeIII GGCC 1 cut(s) 191
Hin1II CATG 2 cut(s) 199, 241
HindIII AAGCTT 1 cut(s) 147
HinfI GANTC 2 cut(s) 44, 108
HphI GGTGA 1 cut(s) 71
Hpy188I TCNGA 3 cut(s) 87, 234, 285
Hpy188III TCNNGA 2 cut(s) 41, 208
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 2 cut(s) 123, 214
HpyCH4III ACNGT 2 cut(s) 80, 159
HpyCH4IV ACGT 2 cut(s) 172, 183
HpyCH4V TGCA 2 cut(s) 162, 245
HpySE526I ACGT 2 cut(s) 172, 183
Hsp92II CATG 2 cut(s) 199, 241
LpnPI CCDG 4 cut(s) 20, 26, 47, 205
MaeI CTAG 2 cut(s) 12, 270
MaeII ACGT 2 cut(s) 172, 183
MaeIII GTNAC 4 cut(s) 59, 168, 173, 184
MluCI AATT 4 cut(s) 71, 101, 134, 275
MlyI GAGTC 1 cut(s) 102
MnlI CCTC 2 cut(s) 61, 211
MseI TTAA 1 cut(s) 144
MslI CAYNNNNRTG 1 cut(s) 52
MspR9I CCNGG 1 cut(s) 35
MvaI CCWGG 1 cut(s) 35
NlaIII CATG 2 cut(s) 199, 241
NmuCI GTSAC 3 cut(s) 59, 173, 184
NspI RCATGY 1 cut(s) 199
OliI CACNNNNGTG 1 cut(s) 52
PaeI GCATGC 1 cut(s) 199
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PfeI GAWTC 1 cut(s) 44
PleI GAGTC 1 cut(s) 102
PmaCI CACGTG 1 cut(s) 184
PmlI CACGTG 1 cut(s) 184
PpsI GAGTC 1 cut(s) 102
Ppu21I YACGTR 1 cut(s) 184
PsiI TTATAA 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 33
PspCI CACGTG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 33
RsaI GTAC 1 cut(s) 156
RsaNI GTAC 1 cut(s) 155
RseI CAYNNNNRTG 1 cut(s) 52
SaqAI TTAA 1 cut(s) 144
SchI GAGTC 1 cut(s) 102
ScrFI CCNGG 1 cut(s) 35
SetI ASST 5 cut(s) 39, 56, 151, 175, 186
SmiMI CAYNNNNRTG 1 cut(s) 52
SphI GCATGC 1 cut(s) 199
Sse9I AATT 4 cut(s) 71, 101, 134, 275
SsiI CCGC 1 cut(s) 294
SspMI CTAG 2 cut(s) 12, 270
StyD4I CCNGG 1 cut(s) 33
TaaI ACNGT 2 cut(s) 80, 159
TaiI ACGT 2 cut(s) 175, 186
TaqI TCGA 1 cut(s) 75
TasI AATT 4 cut(s) 71, 101, 134, 275
TfiI GAWTC 1 cut(s) 44
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TseFI GTSAC 3 cut(s) 59, 173, 184
Tsp45I GTSAC 3 cut(s) 59, 173, 184
TspDTI ATGAA 1 cut(s) 42
TspGWI ACGGA 1 cut(s) 142
XapI RAATTY 2 cut(s) 71, 275
XceI RCATGY 1 cut(s) 199
XspI CTAG 2 cut(s) 12, 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.