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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 133 |
| AcsI | RAATTY | 1 cut(s) | 71 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 40 |
| AjnI | CCWGG | 1 cut(s) | 33 |
| AleI | CACNNNNGTG | 1 cut(s) | 52 |
| ApoI | RAATTY | 1 cut(s) | 71 |
| AsuHPI | GGTGA | 2 cut(s) | 71, 103 |
| BciT130I | CCWGG | 1 cut(s) | 35 |
| BfaI | CTAG | 1 cut(s) | 12 |
| Bme1390I | CCNGG | 1 cut(s) | 35 |
| BmrFI | CCNGG | 1 cut(s) | 35 |
| BpmI | CTGGAG | 1 cut(s) | 17 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 40 |
| BseBI | CCWGG | 1 cut(s) | 35 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 40 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 40 |
| Bst2UI | CCWGG | 1 cut(s) | 35 |
| Bst4CI | ACNGT | 1 cut(s) | 80 |
| BstNI | CCWGG | 1 cut(s) | 35 |
| BstSCI | CCNGG | 1 cut(s) | 33 |
| EcoRI | GAATTC | 1 cut(s) | 71 |
| EcoRII | CCWGG | 1 cut(s) | 33 |
| FaiI | YATR | 1 cut(s) | 133 |
| FspBI | CTAG | 1 cut(s) | 12 |
| GsuI | CTGGAG | 1 cut(s) | 17 |
| HinfI | GANTC | 2 cut(s) | 44, 108 |
| HphI | GGTGA | 2 cut(s) | 71, 103 |
| Hpy188I | TCNGA | 1 cut(s) | 87 |
| Hpy188III | TCNNGA | 1 cut(s) | 41 |
| Hpy99I | CGWCG | 1 cut(s) | 80 |
| HpyAV | CCTTC | 1 cut(s) | 124 |
| HpyCH4III | ACNGT | 1 cut(s) | 80 |
| LpnPI | CCDG | 3 cut(s) | 20, 26, 47 |
| MaeI | CTAG | 1 cut(s) | 12 |
| MaeIII | GTNAC | 1 cut(s) | 59 |
| MluCI | AATT | 2 cut(s) | 71, 101 |
| MlyI | GAGTC | 1 cut(s) | 102 |
| MnlI | CCTC | 1 cut(s) | 61 |
| MslI | CAYNNNNRTG | 1 cut(s) | 52 |
| MspR9I | CCNGG | 1 cut(s) | 35 |
| MvaI | CCWGG | 1 cut(s) | 35 |
| NmuCI | GTSAC | 1 cut(s) | 59 |
| OliI | CACNNNNGTG | 1 cut(s) | 52 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 72 |
| PfeI | GAWTC | 1 cut(s) | 44 |
| PleI | GAGTC | 1 cut(s) | 102 |
| PpsI | GAGTC | 1 cut(s) | 102 |
| PsiI | TTATAA | 1 cut(s) | 133 |
| Psp6I | CCWGG | 1 cut(s) | 33 |
| PspGI | CCWGG | 1 cut(s) | 33 |
| RseI | CAYNNNNRTG | 1 cut(s) | 52 |
| SchI | GAGTC | 1 cut(s) | 102 |
| ScrFI | CCNGG | 1 cut(s) | 35 |
| SetI | ASST | 3 cut(s) | 39, 56, 116 |
| SgeI | CNNG | 6 cut(s) | 24, 46, 47, 53, 79, 130 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 52 |
| Sse9I | AATT | 2 cut(s) | 71, 101 |
| SspMI | CTAG | 1 cut(s) | 12 |
| StyD4I | CCNGG | 1 cut(s) | 33 |
| TaaI | ACNGT | 1 cut(s) | 80 |
| TaqI | TCGA | 1 cut(s) | 75 |
| TasI | AATT | 2 cut(s) | 71, 101 |
| TfiI | GAWTC | 1 cut(s) | 44 |
| TseFI | GTSAC | 1 cut(s) | 59 |
| Tsp45I | GTSAC | 1 cut(s) | 59 |
| XapI | RAATTY | 1 cut(s) | 71 |
| XspI | CTAG | 1 cut(s) | 12 |