MD14G1031100.v1.1

Vacuolar protein sorting-associated protein 55 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010180
Physical Location & Seq
Reverse (-)
2779532 .. 2782702
3171 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1031100.v1.1.491

Sequence Viewer

Length: 279 bp
ATGTTATCTGCTCTGATGTACGTGCTGGTACCCATGCCTTGCTTATTCTTTGGTGGTGGTTCTACTCAGGTTTTATTTACGAGGGAAAGCGATGGGTGGATAAATGCAGCTAAATTCTTGACTGGTGCATCAACTGTTGGGAGCATAGCCATTCCCATCATTCTTAGGCACGCTCATATGATTGAGACACCTGCTATGTGGATTGAATTCTTTTCGTTCTTCATATTTGTCTGTACTGTCATGTGTTTCCACCGGGCTAGTCTCGAAGACGAGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps55

Protein Analysis

93

Amino Acids

10.36

Weight (kDa)

5.38

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps55 PF04133 1 - 89 5.7e-19 Vacuolar protein sorting 55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 199
Acc36I ACCTGC 1 cut(s) 199
Acc65I GGTACC 1 cut(s) 28
AccB1I GGYRCC 1 cut(s) 28
AcsI RAATTY 2 cut(s) 113, 206
AfaI GTAC 3 cut(s) 20, 30, 235
AgsI TTSAA 1 cut(s) 206
AluBI AGCT 1 cut(s) 110
AluI AGCT 1 cut(s) 110
Alw26I GTCTC 2 cut(s) 179, 266
ApeKI GCWGC 1 cut(s) 107
ApoI RAATTY 2 cut(s) 113, 206
Asp718I GGTACC 1 cut(s) 28
AsuC2I CCSGG 1 cut(s) 254
BanI GGYRCC 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 273
BbvI GCAGC 1 cut(s) 119
BccI CCATC 2 cut(s) 86, 164
BcnI CCSGG 1 cut(s) 254
BcoDI GTCTC 2 cut(s) 179, 266
BfaI CTAG 1 cut(s) 258
BfuAI ACCTGC 1 cut(s) 199
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 254
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 1 cut(s) 254
BmsI GCATC 1 cut(s) 137
BpiI GAAGAC 1 cut(s) 273
BpuMI CCSGG 1 cut(s) 254
BsaAI YACGTR 1 cut(s) 22
Bse1I ACTGG 1 cut(s) 127
BseMII CTCAG 1 cut(s) 80
BseNI ACTGG 1 cut(s) 127
BseXI GCAGC 1 cut(s) 119
BshNI GGYRCC 1 cut(s) 28
BsiSI CCGG 1 cut(s) 253
BsmAI GTCTC 2 cut(s) 179, 266
BspCNI CTCAG 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 30
BspMI ACCTGC 1 cut(s) 199
BspT107I GGYRCC 1 cut(s) 28
BsrI ACTGG 1 cut(s) 127
Bst4CI ACNGT 2 cut(s) 136, 238
BstBAI YACGTR 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 2 cut(s) 66, 164
BstMAI GTCTC 2 cut(s) 179, 266
BstSCI CCNGG 1 cut(s) 252
BstV1I GCAGC 1 cut(s) 119
BstV2I GAAGAC 1 cut(s) 273
BtgZI GCGATG 1 cut(s) 105
BveI ACCTGC 1 cut(s) 199
Cac8I GCNNGC 1 cut(s) 171
Csp6I GTAC 3 cut(s) 19, 29, 234
CviAII CATG 2 cut(s) 34, 241
CviJI RGCY 3 cut(s) 110, 149, 257
CviKI_1 RGCY 3 cut(s) 110, 149, 257
CviQI GTAC 3 cut(s) 19, 29, 234
DdeI CTNAG 2 cut(s) 66, 164
EcoRI GAATTC 1 cut(s) 206
FaeI CATG 2 cut(s) 37, 244
FaiI YATR 7 cut(s) 35, 146, 177, 179, 197, 224, 242
FatI CATG 2 cut(s) 33, 240
FauNDI CATATG 1 cut(s) 177
Fnu4HI GCNGC 1 cut(s) 108
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 1 cut(s) 258
GluI GCNGC 1 cut(s) 108
HapII CCGG 1 cut(s) 253
Hin1II CATG 2 cut(s) 37, 244
HpaII CCGG 1 cut(s) 253
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 2 cut(s) 118, 263
HpyCH4III ACNGT 2 cut(s) 136, 238
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 2 cut(s) 107, 128
HpyF3I CTNAG 2 cut(s) 66, 164
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 2 cut(s) 37, 244
KpnI GGTACC 1 cut(s) 32
LmnI GCTCC 1 cut(s) 141
LpnPI CCDG 5 cut(s) 11, 53, 108, 204, 266
Lsp1109I GCAGC 1 cut(s) 119
LweI GCATC 1 cut(s) 137
MaeI CTAG 1 cut(s) 258
MaeII ACGT 1 cut(s) 21
MboII GAAGA 2 cut(s) 211, 278
MluCI AATT 2 cut(s) 113, 206
MnlI CCTC 1 cut(s) 75
MspI CCGG 1 cut(s) 253
MspR9I CCNGG 1 cut(s) 254
NciI CCSGG 1 cut(s) 254
NdeI CATATG 1 cut(s) 177
NlaIII CATG 2 cut(s) 37, 244
NlaIV GGNNCC 1 cut(s) 30
PaqCI CACCTGC 1 cut(s) 199
PkrI GCNGC 1 cut(s) 109
Ppu21I YACGTR 1 cut(s) 22
PspN4I GGNNCC 1 cut(s) 30
RsaI GTAC 3 cut(s) 20, 30, 235
RsaNI GTAC 3 cut(s) 19, 29, 234
SatI GCNGC 1 cut(s) 108
ScrFI CCNGG 1 cut(s) 254
SetI ASST 4 cut(s) 24, 72, 112, 193
SfaNI GCATC 1 cut(s) 137
Sse9I AATT 2 cut(s) 113, 206
SspMI CTAG 1 cut(s) 258
StyD4I CCNGG 1 cut(s) 252
TaaI ACNGT 2 cut(s) 136, 238
TaiI ACGT 1 cut(s) 24
TaqI TCGA 1 cut(s) 264
TasI AATT 2 cut(s) 113, 206
TatI WGTACW 1 cut(s) 233
TseI GCWGC 1 cut(s) 107
TspDTI ATGAA 1 cut(s) 211
XapI RAATTY 2 cut(s) 113, 206
XspI CTAG 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.