pycom12g02850

Vacuolar protein sorting-associated protein 55 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010180
Physical Location & Seq
Reverse (-)
2584283 .. 2585613
1331 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02850.1

Sequence Viewer

Length: 279 bp
ATGTTATCTGCTCTGATGTACGTCGTGGTACCCATGCCTTGCTTATTCTTTGGTGGCGGTTCTACTCAGTTTTTGCTTACGAGGGAAAGTGATGGGTGGATAAATGCTGCTAAATTCCTGACTGGGGCATCAACTGTTGGGAGCATAGCCATTCCTATCATTCTTAGGCACGCTCATATGATTGAGACAGCTGCAATGTGGATTGAATTCTTTTCATTCTTCATATTTATCTGTACTGTCATGTGTTTCCACCGTGCTAGTCTCGAAGATGAGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps55

Protein Analysis

93

Amino Acids

10.34

Weight (kDa)

5.38

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps55 PF04133 1 - 89 1.1e-18 Vacuolar protein sorting 55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 28
AccB1I GGYRCC 1 cut(s) 28
AciI CCGC 1 cut(s) 57
AcsI RAATTY 2 cut(s) 113, 206
AfaI GTAC 3 cut(s) 20, 30, 235
AfiI CCNNNNNNNGG 1 cut(s) 124
AgsI TTSAA 1 cut(s) 206
AluBI AGCT 1 cut(s) 191
AluI AGCT 1 cut(s) 191
Alw26I GTCTC 2 cut(s) 179, 266
ApeKI GCWGC 2 cut(s) 107, 191
ApoI RAATTY 2 cut(s) 113, 206
Asp718I GGTACC 1 cut(s) 28
BanI GGYRCC 1 cut(s) 28
BbvI GCAGC 2 cut(s) 94, 178
BccI CCATC 1 cut(s) 86
BcoDI GTCTC 2 cut(s) 179, 266
BfaI CTAG 1 cut(s) 258
BisI GCNGC 2 cut(s) 108, 192
BlsI GCNGC 2 cut(s) 109, 193
BmiI GGNNCC 1 cut(s) 30
BmrI ACTGGG 1 cut(s) 132
BmsI GCATC 1 cut(s) 137
BmuI ACTGGG 1 cut(s) 132
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse1I ACTGG 1 cut(s) 127
Bse3DI GCAATG 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMI GCAATG 1 cut(s) 201
BseMII CTCAG 1 cut(s) 80
BseNI ACTGG 1 cut(s) 127
BseXI GCAGC 2 cut(s) 94, 178
BshNI GGYRCC 1 cut(s) 28
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 2 cut(s) 179, 266
BspACI CCGC 1 cut(s) 57
BspCNI CTCAG 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 30
BspT107I GGYRCC 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 201
BsrI ACTGG 1 cut(s) 127
Bst4CI ACNGT 3 cut(s) 136, 238, 254
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 2 cut(s) 66, 164
BstMAI GTCTC 2 cut(s) 179, 266
BstV1I GCAGC 2 cut(s) 94, 178
Cac8I GCNNGC 1 cut(s) 171
Csp6I GTAC 3 cut(s) 19, 29, 234
CviAII CATG 2 cut(s) 34, 241
CviJI RGCY 2 cut(s) 149, 191
CviKI_1 RGCY 2 cut(s) 149, 191
CviQI GTAC 3 cut(s) 19, 29, 234
DdeI CTNAG 2 cut(s) 66, 164
EcoRI GAATTC 1 cut(s) 206
FaeI CATG 2 cut(s) 37, 244
FaiI YATR 6 cut(s) 35, 146, 177, 179, 224, 242
FatI CATG 2 cut(s) 33, 240
FauNDI CATATG 1 cut(s) 177
Fnu4HI GCNGC 2 cut(s) 108, 192
Fsp4HI GCNGC 2 cut(s) 108, 192
FspBI CTAG 1 cut(s) 258
GluI GCNGC 2 cut(s) 108, 192
Hin1II CATG 2 cut(s) 37, 244
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 2 cut(s) 118, 263
Hpy99I CGWCG 1 cut(s) 26
HpyCH4III ACNGT 3 cut(s) 136, 238, 254
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 1 cut(s) 194
HpyF3I CTNAG 2 cut(s) 66, 164
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 2 cut(s) 37, 244
KpnI GGTACC 1 cut(s) 32
LmnI GCTCC 1 cut(s) 141
LpnPI CCDG 2 cut(s) 108, 131
Lsp1109I GCAGC 2 cut(s) 94, 178
LweI GCATC 1 cut(s) 137
MaeI CTAG 1 cut(s) 258
MaeII ACGT 1 cut(s) 21
MboII GAAGA 2 cut(s) 211, 278
MluCI AATT 2 cut(s) 113, 206
MnlI CCTC 1 cut(s) 75
MspA1I CMGCKG 1 cut(s) 191
NdeI CATATG 1 cut(s) 177
NlaIII CATG 2 cut(s) 37, 244
NlaIV GGNNCC 1 cut(s) 30
PkrI GCNGC 2 cut(s) 109, 193
PspN4I GGNNCC 1 cut(s) 30
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 3 cut(s) 20, 30, 235
RsaNI GTAC 3 cut(s) 19, 29, 234
SatI GCNGC 2 cut(s) 108, 192
SetI ASST 2 cut(s) 24, 193
SfaNI GCATC 1 cut(s) 137
Sse9I AATT 2 cut(s) 113, 206
SsiI CCGC 1 cut(s) 57
SspMI CTAG 1 cut(s) 258
TaaI ACNGT 3 cut(s) 136, 238, 254
TaiI ACGT 1 cut(s) 24
TaqI TCGA 1 cut(s) 264
TasI AATT 2 cut(s) 113, 206
TatI WGTACW 1 cut(s) 233
TseI GCWGC 2 cut(s) 107, 191
TspDTI ATGAA 2 cut(s) 204, 211
XapI RAATTY 2 cut(s) 113, 206
XspI CTAG 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.