Rh3DG219200

Vacuolar protein sorting-associated protein 55 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0010180
Physical Location & Seq
Reverse (-)
20403503 .. 20407327
3825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG219200.1

Sequence Viewer

Length: 420 bp
ATGGCGATGACCATAGAAGACACTTTGGCTCAGAAAGAGAAGGGTTCCATTGTTCGACTTTTTATATGCAATGATGATAATTCATGTGAACACATTCATATTTTCATGACATTGGTTGCTAACTATTTTCTGTTCTGGTGCATTGTTGCTCCACAAATTGTTGACGCTTCGTGCTCTTTGCAGTGCTTGCAGGACTTGCTTTTATGTTTTCAGCGAGCATCTTGCTACAGGTTCTGGTGGATAAATGCTGCCAAGTTTTTGACTGGGGCATCAACTGTGGGGAGCCTAGCCATTCCTATAATTCTTAGGCACGCTCATATGATTGAGACATCTGCTATGTGGATCGAATTCGTATCATTCTTCATATTTGTGTGCACTGTCATGTGTTTCCATCGTGCCAGCCTCGAAGACGAGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps55

Protein Analysis

139

Amino Acids

16.02

Weight (kDa)

5.38

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps55 PF04133 79 - 135 2.5e-11 Vacuolar protein sorting 55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 350
AcsI RAATTY 1 cut(s) 347
Alw21I GWGCWC 2 cut(s) 176, 377
Alw26I GTCTC 1 cut(s) 320
Alw44I GTGCAC 1 cut(s) 373
AlwI GGATC 1 cut(s) 350
AlwNI CAGNNNCTG 1 cut(s) 234
ApaLI GTGCAC 1 cut(s) 373
ApeKI GCWGC 1 cut(s) 248
ApoI RAATTY 1 cut(s) 347
Asp700I GAANNNNTTC 1 cut(s) 93
BaeGI GKGCMC 1 cut(s) 377
BbsI GAAGAC 2 cut(s) 24, 414
Bbv12I GWGCWC 2 cut(s) 176, 377
BbvI GCAGC 1 cut(s) 235
BccI CCATC 1 cut(s) 399
BcgI CGANNNNNNTGC 2 cut(s) 204, 238
BcoDI GTCTC 1 cut(s) 320
BfaI CTAG 1 cut(s) 287
BfmI CTRYAG 1 cut(s) 226
BisI GCNGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 250
BmiI GGNNCC 2 cut(s) 46, 284
BmrI ACTGGG 1 cut(s) 273
BmsI GCATC 2 cut(s) 227, 278
BmuI ACTGGG 1 cut(s) 273
BpiI GAAGAC 2 cut(s) 24, 414
Bse1I ACTGG 1 cut(s) 268
Bse3DI GCAATG 1 cut(s) 76
BseMI GCAATG 1 cut(s) 76
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 1 cut(s) 268
BseSI GKGCMC 1 cut(s) 377
BseXI GCAGC 1 cut(s) 235
BsiHKAI GWGCWC 2 cut(s) 176, 377
BsmAI GTCTC 1 cut(s) 320
Bsp1286I GDGCHC 2 cut(s) 176, 377
Bsp143I GATC 1 cut(s) 342
BspCNI CTCAG 1 cut(s) 43
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 2 cut(s) 46, 284
BspPI GGATC 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 76
BsrI ACTGG 1 cut(s) 268
BssMI GATC 1 cut(s) 342
Bst4CI ACNGT 2 cut(s) 277, 379
BstAPI GCANNNNNTGC 2 cut(s) 187, 196
BstC8I GCNNGC 4 cut(s) 188, 216, 312, 400
BstDEI CTNAG 2 cut(s) 30, 305
BstKTI GATC 1 cut(s) 345
BstMAI GTCTC 1 cut(s) 320
BstMBI GATC 1 cut(s) 342
BstMWI GCNNNNNNNGC 2 cut(s) 187, 196
BstSFI CTRYAG 1 cut(s) 226
BstSLI GKGCMC 1 cut(s) 377
BstV1I GCAGC 1 cut(s) 235
BstV2I GAAGAC 2 cut(s) 24, 414
BtgZI GCGATG 1 cut(s) 20
BtsI GCAGTG 1 cut(s) 188
BtsIMutI CAGTG 2 cut(s) 188, 375
Cac8I GCNNGC 4 cut(s) 188, 216, 312, 400
CaiI CAGNNNCTG 1 cut(s) 234
CciI TCATGA 1 cut(s) 105
CseI GACGC 1 cut(s) 173
CviAII CATG 3 cut(s) 84, 106, 382
CviJI RGCY 4 cut(s) 29, 285, 290, 402
CviKI_1 RGCY 4 cut(s) 29, 285, 290, 402
DdeI CTNAG 2 cut(s) 30, 305
DpnI GATC 1 cut(s) 344
DpnII GATC 1 cut(s) 342
EcoRI GAATTC 1 cut(s) 347
FaeI CATG 3 cut(s) 87, 109, 385
FatI CATG 3 cut(s) 83, 105, 381
FauNDI CATATG 1 cut(s) 318
Fnu4HI GCNGC 1 cut(s) 249
Fsp4HI GCNGC 1 cut(s) 249
FspBI CTAG 1 cut(s) 287
GluI GCNGC 1 cut(s) 249
HgaI GACGC 1 cut(s) 173
Hin1II CATG 3 cut(s) 87, 109, 385
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
Hpy166II GTNNAC 3 cut(s) 89, 163, 375
Hpy188I TCNGA 1 cut(s) 33
Hpy188III TCNNGA 1 cut(s) 106
Hpy8I GTNNAC 3 cut(s) 89, 163, 375
HpyAV CCTTC 1 cut(s) 34
HpyCH4III ACNGT 2 cut(s) 277, 379
HpyCH4V TGCA 5 cut(s) 69, 141, 181, 190, 375
HpyF10VI GCNNNNNNNGC 2 cut(s) 187, 196
HpyF3I CTNAG 2 cut(s) 30, 305
Hsp92II CATG 3 cut(s) 87, 109, 385
Kzo9I GATC 1 cut(s) 342
LmnI GCTCC 2 cut(s) 154, 282
LpnPI CCDG 6 cut(s) 121, 176, 214, 220, 249, 412
Lsp1109I GCAGC 1 cut(s) 235
LweI GCATC 2 cut(s) 227, 278
MaeI CTAG 1 cut(s) 287
MalI GATC 1 cut(s) 344
MboI GATC 1 cut(s) 342
MboII GAAGA 3 cut(s) 29, 352, 419
MhlI GDGCHC 2 cut(s) 176, 377
MluCI AATT 4 cut(s) 79, 156, 300, 347
MnlI CCTC 1 cut(s) 413
MroXI GAANNNNTTC 1 cut(s) 93
MslI CAYNNNNRTG 2 cut(s) 368, 380
MwoI GCNNNNNNNGC 2 cut(s) 187, 196
NdeI CATATG 1 cut(s) 318
NdeII GATC 1 cut(s) 342
NlaIII CATG 3 cut(s) 87, 109, 385
NlaIV GGNNCC 2 cut(s) 46, 284
PagI TCATGA 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 93
PkrI GCNGC 1 cut(s) 250
PspN4I GGNNCC 2 cut(s) 46, 284
PstNI CAGNNNCTG 1 cut(s) 234
RseI CAYNNNNRTG 2 cut(s) 368, 380
SatI GCNGC 1 cut(s) 249
Sau3AI GATC 1 cut(s) 342
SduI GDGCHC 2 cut(s) 176, 377
SetI ASST 1 cut(s) 233
SfaNI GCATC 2 cut(s) 227, 278
SfcI CTRYAG 1 cut(s) 226
SmiMI CAYNNNNRTG 2 cut(s) 368, 380
Sse9I AATT 4 cut(s) 79, 156, 300, 347
SspMI CTAG 1 cut(s) 287
TaaI ACNGT 2 cut(s) 277, 379
TaqI TCGA 3 cut(s) 55, 345, 405
TasI AATT 4 cut(s) 79, 156, 300, 347
TscAI CASTG 2 cut(s) 188, 382
TseI GCWGC 1 cut(s) 248
TspDTI ATGAA 4 cut(s) 72, 86, 94, 352
TspRI CASTG 2 cut(s) 188, 382
VneI GTGCAC 1 cut(s) 373
XapI RAATTY 1 cut(s) 347
XmnI GAANNNNTTC 1 cut(s) 93
XspI CTAG 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.