Rh3CG218000

Vacuolar protein sorting-associated protein 55 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0010180
Physical Location & Seq
Reverse (-)
20269156 .. 20274912
5757 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG218000.1

Sequence Viewer

Length: 279 bp
ATGTTGTCTGCTCTCATGTACGTCCTGGTACCGATGCCTTGTCTATTCTTTGGTGGTGGTTCTACTCAGGTTTTATTTACGAGGGAGAGTGATGGGTGGATAAATGCTGCCAAGTTTTTGACTGGGGCATCAACTGTGGGGAGCCTAGCCATTCCTATAATTCTTAGGCACGCTCATATGATTGAGACATCTGCTATGTGGATCGAATTCGTATCATTCTTCATATTTGTGTGCACTGTCATGTGTTTCCATCGTGCCAGCCTCGAAGACGAGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps55

Protein Analysis

92

Amino Acids

10.3

Weight (kDa)

5.38

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps55 PF04133 1 - 89 6.9e-19 Vacuolar protein sorting 55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 28
AccB1I GGYRCC 1 cut(s) 28
AclWI GGATC 1 cut(s) 209
AcsI RAATTY 1 cut(s) 206
AfaI GTAC 2 cut(s) 20, 30
AjnI CCWGG 1 cut(s) 24
Alw21I GWGCWC 1 cut(s) 236
Alw26I GTCTC 1 cut(s) 179
Alw44I GTGCAC 1 cut(s) 232
AlwI GGATC 1 cut(s) 209
ApaLI GTGCAC 1 cut(s) 232
ApeKI GCWGC 1 cut(s) 107
ApoI RAATTY 1 cut(s) 206
Asp718I GGTACC 1 cut(s) 28
BaeGI GKGCMC 1 cut(s) 236
BanI GGYRCC 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 273
Bbv12I GWGCWC 1 cut(s) 236
BbvI GCAGC 1 cut(s) 94
BccI CCATC 2 cut(s) 86, 258
BciT130I CCWGG 1 cut(s) 26
BcoDI GTCTC 1 cut(s) 179
BfaI CTAG 1 cut(s) 146
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 26
BmiI GGNNCC 2 cut(s) 30, 143
BmrFI CCNGG 1 cut(s) 26
BmrI ACTGGG 1 cut(s) 132
BmsI GCATC 2 cut(s) 24, 137
BmuI ACTGGG 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 273
Bse1I ACTGG 1 cut(s) 127
BseBI CCWGG 1 cut(s) 26
BseMII CTCAG 1 cut(s) 80
BseNI ACTGG 1 cut(s) 127
BseSI GKGCMC 1 cut(s) 236
BseXI GCAGC 1 cut(s) 94
BshNI GGYRCC 1 cut(s) 28
BsiHKAI GWGCWC 1 cut(s) 236
BsmAI GTCTC 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 236
Bsp143I GATC 1 cut(s) 201
BspCNI CTCAG 1 cut(s) 79
BspLI GGNNCC 2 cut(s) 30, 143
BspPI GGATC 1 cut(s) 209
BspT107I GGYRCC 1 cut(s) 28
BsrI ACTGG 1 cut(s) 127
BssMI GATC 1 cut(s) 201
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 2 cut(s) 136, 238
BstC8I GCNNGC 2 cut(s) 171, 259
BstDEI CTNAG 2 cut(s) 66, 164
BstKTI GATC 1 cut(s) 204
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 1 cut(s) 201
BstNI CCWGG 1 cut(s) 26
BstSCI CCNGG 1 cut(s) 24
BstSLI GKGCMC 1 cut(s) 236
BstV1I GCAGC 1 cut(s) 94
BstV2I GAAGAC 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 2 cut(s) 171, 259
Csp6I GTAC 2 cut(s) 19, 29
CviAII CATG 2 cut(s) 16, 241
CviJI RGCY 3 cut(s) 144, 149, 261
CviKI_1 RGCY 3 cut(s) 144, 149, 261
CviQI GTAC 2 cut(s) 19, 29
DdeI CTNAG 2 cut(s) 66, 164
DpnI GATC 1 cut(s) 203
DpnII GATC 1 cut(s) 201
EcoRI GAATTC 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 24
FaeI CATG 2 cut(s) 19, 244
FaiI YATR 7 cut(s) 17, 158, 177, 179, 197, 224, 242
FatI CATG 2 cut(s) 15, 240
FauNDI CATATG 1 cut(s) 177
Fnu4HI GCNGC 1 cut(s) 108
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 1 cut(s) 146
GluI GCNGC 1 cut(s) 108
Hin1II CATG 2 cut(s) 19, 244
Hpy166II GTNNAC 1 cut(s) 234
Hpy8I GTNNAC 1 cut(s) 234
HpyCH4III ACNGT 2 cut(s) 136, 238
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 1 cut(s) 234
HpyF3I CTNAG 2 cut(s) 66, 164
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 2 cut(s) 19, 244
KpnI GGTACC 1 cut(s) 32
Kzo9I GATC 1 cut(s) 201
LmnI GCTCC 1 cut(s) 141
LpnPI CCDG 5 cut(s) 11, 38, 53, 108, 271
Lsp1109I GCAGC 1 cut(s) 94
LweI GCATC 2 cut(s) 24, 137
MaeI CTAG 1 cut(s) 146
MaeII ACGT 1 cut(s) 21
MalI GATC 1 cut(s) 203
MboI GATC 1 cut(s) 201
MboII GAAGA 2 cut(s) 211, 278
MhlI GDGCHC 1 cut(s) 236
MluCI AATT 2 cut(s) 159, 206
MnlI CCTC 2 cut(s) 75, 272
MslI CAYNNNNRTG 2 cut(s) 227, 239
MspR9I CCNGG 1 cut(s) 26
MvaI CCWGG 1 cut(s) 26
NdeI CATATG 1 cut(s) 177
NdeII GATC 1 cut(s) 201
NlaIII CATG 2 cut(s) 19, 244
NlaIV GGNNCC 2 cut(s) 30, 143
PkrI GCNGC 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 24
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 2 cut(s) 30, 143
RsaI GTAC 2 cut(s) 20, 30
RsaNI GTAC 2 cut(s) 19, 29
RseI CAYNNNNRTG 2 cut(s) 227, 239
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 1 cut(s) 201
ScrFI CCNGG 1 cut(s) 26
SduI GDGCHC 1 cut(s) 236
SetI ASST 2 cut(s) 24, 72
SfaNI GCATC 2 cut(s) 24, 137
SmiMI CAYNNNNRTG 2 cut(s) 227, 239
Sse9I AATT 2 cut(s) 159, 206
SspMI CTAG 1 cut(s) 146
StyD4I CCNGG 1 cut(s) 24
TaaI ACNGT 2 cut(s) 136, 238
TaiI ACGT 1 cut(s) 24
TaqI TCGA 2 cut(s) 204, 264
TasI AATT 2 cut(s) 159, 206
TscAI CASTG 1 cut(s) 241
TseI GCWGC 1 cut(s) 107
TspDTI ATGAA 1 cut(s) 211
TspRI CASTG 1 cut(s) 241
VneI GTGCAC 1 cut(s) 232
XapI RAATTY 1 cut(s) 206
XspI CTAG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.