RLG00000023899

Vacuolar protein sorting-associated protein 55 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0010180
Physical Location & Seq
Forward (+)
29693133 .. 29696951
3819 bp
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UTR
Exon/CDS
Intron
RLM00000023899

Sequence Viewer

Length: 423 bp
ATGGCGATGACCATAGAAGCTTCTCTGAAGCATACTTATGAAGTCACTTTCTTAATGTGCAGGCTTGCAGGACTTGCTTTTATGTTTTCAGCGAGCATCTTGCTACAGGTTTTGGCTTGTGCATTATACAACAATTGGTGGCCTATGTTGTCTGCTCTCATGTACGTCCTGGTACCGATGCCTTGTCTATTCTTTGGTGGTGGTTCTACTCAGGTTTTATTTACGAGGGAGAGTGATGGGTGGATAAATGCTGCTAAGTTTTTGACTGGGGCATCAACTGTGGGGAGCCTAGCCATTCCTATAATTCTTAGGCACGCTCATATGATTGAGACATCTGCTATGTGGATCGAATTCGTATCATTCTTCATATTTGTGTGCACTGTCATGTGTTTCCATCGTGCCAGCCTCGAAGACGAGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps55

Protein Analysis

141

Amino Acids

15.75

Weight (kDa)

5.7

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps55 PF04133 23 - 136 6.9e-32 Vacuolar protein sorting 55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 172
AccB1I GGYRCC 1 cut(s) 172
AclWI GGATC 1 cut(s) 353
AcsI RAATTY 1 cut(s) 350
AcuI CTGAAG 1 cut(s) 47
AfaI GTAC 2 cut(s) 164, 174
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 1 cut(s) 20
AluI AGCT 1 cut(s) 20
Alw21I GWGCWC 1 cut(s) 380
Alw26I GTCTC 1 cut(s) 323
Alw44I GTGCAC 1 cut(s) 376
AlwI GGATC 1 cut(s) 353
AoxI GGCC 1 cut(s) 140
ApaLI GTGCAC 1 cut(s) 376
ApeKI GCWGC 1 cut(s) 251
ApoI RAATTY 1 cut(s) 350
Asp718I GGTACC 1 cut(s) 172
BaeGI GKGCMC 1 cut(s) 380
BanI GGYRCC 1 cut(s) 172
BbsI GAAGAC 1 cut(s) 417
Bbv12I GWGCWC 1 cut(s) 380
BbvI GCAGC 1 cut(s) 238
BccI CCATC 2 cut(s) 230, 402
BcgI CGANNNNNNTGC 2 cut(s) 82, 116
BciT130I CCWGG 1 cut(s) 170
BcoDI GTCTC 1 cut(s) 323
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 104
BisI GCNGC 1 cut(s) 252
BlsI GCNGC 1 cut(s) 253
Bme1390I CCNGG 1 cut(s) 170
BmiI GGNNCC 2 cut(s) 174, 287
BmrFI CCNGG 1 cut(s) 170
BmrI ACTGGG 1 cut(s) 276
BmsI GCATC 3 cut(s) 105, 168, 281
BmuI ACTGGG 1 cut(s) 276
BpiI GAAGAC 1 cut(s) 417
Bse1I ACTGG 1 cut(s) 271
BseBI CCWGG 1 cut(s) 170
BseMII CTCAG 1 cut(s) 224
BseNI ACTGG 1 cut(s) 271
BseSI GKGCMC 1 cut(s) 380
BseXI GCAGC 1 cut(s) 238
BsgI GTGCAG 1 cut(s) 79
BshFI GGCC 1 cut(s) 142
BshNI GGYRCC 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 380
BsmAI GTCTC 1 cut(s) 323
BsnI GGCC 1 cut(s) 142
Bsp1286I GDGCHC 1 cut(s) 380
Bsp143I GATC 1 cut(s) 345
BspANI GGCC 1 cut(s) 142
BspCNI CTCAG 1 cut(s) 223
BspLI GGNNCC 2 cut(s) 174, 287
BspPI GGATC 1 cut(s) 353
BspT107I GGYRCC 1 cut(s) 172
BsrI ACTGG 1 cut(s) 271
BssMI GATC 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 280, 382
BstAPI GCANNNNNTGC 1 cut(s) 74
BstC8I GCNNGC 5 cut(s) 62, 66, 94, 315, 403
BstDEI CTNAG 3 cut(s) 210, 255, 308
BstKTI GATC 1 cut(s) 348
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 1 cut(s) 345
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNI CCWGG 1 cut(s) 170
BstSCI CCNGG 1 cut(s) 168
BstSFI CTRYAG 1 cut(s) 104
BstSLI GKGCMC 1 cut(s) 380
BstV1I GCAGC 1 cut(s) 238
BstV2I GAAGAC 1 cut(s) 417
BsuRI GGCC 1 cut(s) 142
BtgZI GCGATG 1 cut(s) 20
BtsIMutI CAGTG 1 cut(s) 378
Cac8I GCNNGC 5 cut(s) 62, 66, 94, 315, 403
Csp6I GTAC 2 cut(s) 163, 173
CviAII CATG 2 cut(s) 160, 385
CviJI RGCY 7 cut(s) 20, 64, 116, 142, 288, 293, 405
CviKI_1 RGCY 7 cut(s) 20, 64, 116, 142, 288, 293, 405
CviQI GTAC 2 cut(s) 163, 173
DdeI CTNAG 3 cut(s) 210, 255, 308
DpnI GATC 1 cut(s) 347
DpnII GATC 1 cut(s) 345
Eco57I CTGAAG 1 cut(s) 47
EcoRI GAATTC 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 168
FaeI CATG 2 cut(s) 163, 388
FatI CATG 2 cut(s) 159, 384
FauNDI CATATG 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 252
Fsp4HI GCNGC 1 cut(s) 252
FspBI CTAG 1 cut(s) 290
GluI GCNGC 1 cut(s) 252
HaeIII GGCC 1 cut(s) 142
Hin1II CATG 2 cut(s) 163, 388
HindIII AAGCTT 1 cut(s) 18
Hpy166II GTNNAC 1 cut(s) 378
Hpy188I TCNGA 1 cut(s) 27
Hpy8I GTNNAC 1 cut(s) 378
HpyCH4III ACNGT 2 cut(s) 280, 382
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 4 cut(s) 60, 68, 122, 378
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 3 cut(s) 210, 255, 308
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 2 cut(s) 163, 388
KpnI GGTACC 1 cut(s) 176
Kzo9I GATC 1 cut(s) 345
LmnI GCTCC 1 cut(s) 285
LpnPI CCDG 8 cut(s) 46, 54, 92, 155, 182, 197, 252, 415
Lsp1109I GCAGC 1 cut(s) 238
LweI GCATC 3 cut(s) 105, 168, 281
MaeI CTAG 1 cut(s) 290
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 1 cut(s) 43
MalI GATC 1 cut(s) 347
MboI GATC 1 cut(s) 345
MboII GAAGA 2 cut(s) 355, 422
MfeI CAATTG 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 380
MluCI AATT 3 cut(s) 133, 303, 350
MnlI CCTC 2 cut(s) 219, 416
MseI TTAA 1 cut(s) 53
MslI CAYNNNNRTG 3 cut(s) 36, 371, 383
MspR9I CCNGG 1 cut(s) 170
MunI CAATTG 1 cut(s) 133
MvaI CCWGG 1 cut(s) 170
MwoI GCNNNNNNNGC 1 cut(s) 74
NdeI CATATG 1 cut(s) 321
NdeII GATC 1 cut(s) 345
NlaIII CATG 2 cut(s) 163, 388
NlaIV GGNNCC 2 cut(s) 174, 287
NmuCI GTSAC 1 cut(s) 43
PkrI GCNGC 1 cut(s) 253
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 2 cut(s) 174, 287
RsaI GTAC 2 cut(s) 164, 174
RsaNI GTAC 2 cut(s) 163, 173
RseI CAYNNNNRTG 3 cut(s) 36, 371, 383
SaqAI TTAA 1 cut(s) 53
SatI GCNGC 1 cut(s) 252
Sau3AI GATC 1 cut(s) 345
ScrFI CCNGG 1 cut(s) 170
SduI GDGCHC 1 cut(s) 380
SetI ASST 4 cut(s) 22, 111, 168, 216
SfaNI GCATC 3 cut(s) 105, 168, 281
SfcI CTRYAG 1 cut(s) 104
SmiMI CAYNNNNRTG 3 cut(s) 36, 371, 383
Sse9I AATT 3 cut(s) 133, 303, 350
SspMI CTAG 1 cut(s) 290
StyD4I CCNGG 1 cut(s) 168
TaaI ACNGT 2 cut(s) 280, 382
TaiI ACGT 1 cut(s) 168
TaqI TCGA 2 cut(s) 348, 408
TasI AATT 3 cut(s) 133, 303, 350
Tru1I TTAA 1 cut(s) 53
Tru9I TTAA 1 cut(s) 53
TscAI CASTG 1 cut(s) 385
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 1 cut(s) 251
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 2 cut(s) 54, 355
TspRI CASTG 1 cut(s) 385
VneI GTGCAC 1 cut(s) 376
XapI RAATTY 1 cut(s) 350
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.