MD14G1037100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012142
Physical Location & Seq
Forward (+)
3414763 .. 3415245
483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1037100.v1.1.491

Sequence Viewer

Length: 483 bp
ATGAATGGTCGTCTTAGCAAGCTCGGGACTGCGCTCACTGTAGTGTTTGCGGTCACTCTCGCTGCCCTCGTAGCGCAGCTCTTCTTCCTCCTCTGGCGGCGAAGGAAGTCCCGGTTTCCGACCCGCATCGGACCCCACGAAGCTCATCATAGCAACCAACCACCTAGCTCACTGCACCGCAACATCGACGTGGACGTGGACGAGGCGTTCAAGTGGCAGCAGGGACTGTACGGCGTGTCGTCGAGGGTGCTGTTCACGATCGCGGAGGAGGAAGAGAGAGAGGGTCTGGACTCGGAGACGCCTACAACGACGTATTCATCTTGTGCGGAGAAAGAGGCGATGACGGCGACGAAGGTGGTACTGGATGTAGCTGTGACTGTGACAGTAGAGGTGGAGGAGGAAACGCCGCCGTTCACGACTCCTTGTGCTTCGCCTCCGTACTATACTCCTTCGCCTTCTCCTAGTCGTGATTTTGTAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

17.76

Weight (kDa)

5.37

Isoelectric Point (pI)

56.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012142)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11640 AT3G11640 AT3G52480
fragaria_vesca FvH4_6g20110
malus_domestica MD14G1037100.v1.1
prunus_persica Prupe.2G218900_v2.0.a1 Prupe.7G097300_v2.0.a1
pyrus_communis pycom12g03290 pycom14g03240
rosa_chinensis RchiOBHm_Chr3g0475571
rosa_laevigata RLG00000023833
rosa_multiflora Rmu_sc0000539.1_g000027
rosa_roxburghii Rroxscaffold_6G00406140
rosa_rugosa Rorug03G0150400
rosa_samantha Rh3AG200000 Rh3BG230400 Rh3CG224700 Rh3DG225400
rosa_wichuraiana Rw3G018140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 263
AciI CCGC 7 cut(s) 50, 97, 124, 178, 263, 326, 407
AcyI GRCGYC 1 cut(s) 299
AfaI GTAC 3 cut(s) 230, 360, 440
AgsI TTSAA 1 cut(s) 211
AjiI CACGTC 2 cut(s) 190, 196
AleI CACNNNNGTG 1 cut(s) 41
AloI GAACNNNNNNTCC 2 cut(s) 191, 223
AluBI AGCT 5 cut(s) 22, 79, 143, 168, 371
AluI AGCT 5 cut(s) 22, 79, 143, 168, 371
Alw26I GTCTC 1 cut(s) 290
AlwNI CAGNNNCTG 1 cut(s) 226
Ama87I CYCGRG 1 cut(s) 23
ApeKI GCWGC 3 cut(s) 62, 76, 217
AspLEI GCGC 2 cut(s) 34, 76
AspS9I GGNCC 1 cut(s) 131
AsuC2I CCSGG 1 cut(s) 112
AvaI CYCGRG 1 cut(s) 23
AvaII GGWCC 1 cut(s) 131
BbvI GCAGC 3 cut(s) 49, 88, 229
BceAI ACGGC 3 cut(s) 247, 360, 394
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 290
BfaI CTAG 2 cut(s) 165, 462
BfmI CTRYAG 1 cut(s) 39
BisI GCNGC 5 cut(s) 63, 77, 98, 218, 407
BlsI GCNGC 5 cut(s) 64, 78, 99, 219, 408
Bme1390I CCNGG 1 cut(s) 112
Bme18I GGWCC 1 cut(s) 131
BmeT110I CYCGRG 1 cut(s) 23
BmgBI CACGTC 2 cut(s) 190, 196
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 133
BmrFI CCNGG 1 cut(s) 112
BmsI GCATC 1 cut(s) 135
BpuMI CCSGG 1 cut(s) 112
BsaHI GRCGYC 1 cut(s) 299
Bse1I ACTGG 1 cut(s) 366
BseGI GGATG 1 cut(s) 370
BseNI ACTGG 1 cut(s) 366
BseRI GAGGAG 3 cut(s) 80, 281, 410
BseXI GCAGC 3 cut(s) 49, 88, 229
BsgI GTGCAG 1 cut(s) 158
Bsh1236I CGCG 1 cut(s) 263
Bsh1285I CGRYCG 1 cut(s) 261
BsiEI CGRYCG 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 23
BsiSI CCGG 1 cut(s) 112
BslFI GGGAC 3 cut(s) 40, 94, 237
BsmAI GTCTC 1 cut(s) 290
BsmBI CGTCTC 1 cut(s) 290
BsmFI GGGAC 3 cut(s) 40, 94, 237
BsoBI CYCGRG 1 cut(s) 23
Bsp143I GATC 1 cut(s) 258
BspACI CCGC 7 cut(s) 50, 97, 124, 178, 263, 326, 407
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 1 cut(s) 133
BspQI GCTCTTC 1 cut(s) 86
BsrI ACTGG 1 cut(s) 366
BssMI GATC 1 cut(s) 258
BssNI GRCGYC 1 cut(s) 299
Bst4CI ACNGT 4 cut(s) 40, 228, 379, 385
Bst6I CTCTTC 2 cut(s) 86, 267
BstACI GRCGYC 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 20
BstDEI CTNAG 1 cut(s) 14
BstF5I GGATG 1 cut(s) 370
BstFNI CGCG 1 cut(s) 263
BstHHI GCGC 2 cut(s) 34, 76
BstKTI GATC 1 cut(s) 261
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 1 cut(s) 258
BstMCI CGRYCG 1 cut(s) 261
BstMWI GCNNNNNNNGC 2 cut(s) 71, 344
BstSCI CCNGG 1 cut(s) 110
BstSFI CTRYAG 1 cut(s) 39
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 3 cut(s) 49, 88, 229
BtgZI GCGATG 1 cut(s) 353
BtrI CACGTC 2 cut(s) 190, 196
BtsCI GGATG 1 cut(s) 370
BtsI GCAGTG 1 cut(s) 170
BtsIMutI CAGTG 2 cut(s) 36, 170
Cac8I GCNNGC 1 cut(s) 20
CaiI CAGNNNCTG 1 cut(s) 226
CfoI GCGC 2 cut(s) 34, 76
Cfr13I GGNCC 1 cut(s) 131
CseI GACGC 1 cut(s) 307
Csp6I GTAC 3 cut(s) 229, 359, 439
CviJI RGCY 5 cut(s) 22, 79, 143, 168, 371
CviKI_1 RGCY 5 cut(s) 22, 79, 143, 168, 371
CviQI GTAC 3 cut(s) 229, 359, 439
DdeI CTNAG 1 cut(s) 14
DpnI GATC 1 cut(s) 260
DpnII GATC 1 cut(s) 258
Eam1104I CTCTTC 2 cut(s) 86, 267
EarI CTCTTC 2 cut(s) 86, 267
Eco47I GGWCC 1 cut(s) 131
Eco88I CYCGRG 1 cut(s) 23
Esp3I CGTCTC 1 cut(s) 290
FaiI YATR 2 cut(s) 150, 444
FaqI GGGAC 3 cut(s) 40, 94, 237
FauI CCCGC 1 cut(s) 131
Fnu4HI GCNGC 5 cut(s) 63, 77, 98, 218, 407
FokI GGATG 1 cut(s) 377
Fsp4HI GCNGC 5 cut(s) 63, 77, 98, 218, 407
FspBI CTAG 2 cut(s) 165, 462
GlaI GCGC 2 cut(s) 33, 75
GluI GCNGC 5 cut(s) 63, 77, 98, 218, 407
HapII CCGG 1 cut(s) 112
HgaI GACGC 1 cut(s) 307
HhaI GCGC 2 cut(s) 34, 76
Hin1I GRCGYC 1 cut(s) 299
Hin6I GCGC 2 cut(s) 32, 74
HinP1I GCGC 2 cut(s) 32, 74
HinfI GANTC 2 cut(s) 290, 418
HpaII CCGG 1 cut(s) 112
Hpy166II GTNNAC 4 cut(s) 193, 199, 255, 414
Hpy188I TCNGA 4 cut(s) 120, 131, 295, 482
Hpy188III TCNNGA 5 cut(s) 25, 256, 287, 415, 467
Hpy8I GTNNAC 4 cut(s) 193, 199, 255, 414
Hpy99I CGWCG 4 cut(s) 191, 244, 313, 352
HpyAV CCTTC 4 cut(s) 96, 346, 459, 465
HpyCH4III ACNGT 4 cut(s) 40, 228, 379, 385
HpyCH4IV ACGT 3 cut(s) 189, 195, 311
HpyCH4V TGCA 1 cut(s) 175
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 344
HpyF3I CTNAG 1 cut(s) 14
HpySE526I ACGT 3 cut(s) 189, 195, 311
Hsp92I GRCGYC 1 cut(s) 299
HspAI GCGC 2 cut(s) 32, 74
Kzo9I GATC 1 cut(s) 258
LguI GCTCTTC 1 cut(s) 86
LpnPI CCDG 5 cut(s) 79, 125, 206, 272, 347
Lsp1109I GCAGC 3 cut(s) 49, 88, 229
LweI GCATC 1 cut(s) 135
MaeI CTAG 2 cut(s) 165, 462
MaeII ACGT 3 cut(s) 189, 195, 311
MaeIII GTNAC 3 cut(s) 52, 373, 379
MalI GATC 1 cut(s) 260
MboI GATC 1 cut(s) 258
MboII GAAGA 3 cut(s) 73, 76, 284
MlyI GAGTC 2 cut(s) 284, 412
MmeI TCCRAC 1 cut(s) 143
MslI CAYNNNNRTG 2 cut(s) 41, 188
MspI CCGG 1 cut(s) 112
MspR9I CCNGG 1 cut(s) 112
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 2 cut(s) 71, 344
NciI CCSGG 1 cut(s) 112
NdeII GATC 1 cut(s) 258
NlaIV GGNNCC 1 cut(s) 133
NmuCI GTSAC 3 cut(s) 52, 373, 379
OliI CACNNNNGTG 1 cut(s) 41
PciSI GCTCTTC 1 cut(s) 86
PcsI WCGNNNNNNNCGW 4 cut(s) 66, 135, 192, 305
PkrI GCNGC 5 cut(s) 64, 78, 99, 219, 408
Ple19I CGATCG 1 cut(s) 261
PleI GAGTC 2 cut(s) 284, 412
PpsI GAGTC 2 cut(s) 284, 412
PspN4I GGNNCC 1 cut(s) 133
PspPI GGNCC 1 cut(s) 131
PstNI CAGNNNCTG 1 cut(s) 226
PvuI CGATCG 1 cut(s) 261
RsaI GTAC 3 cut(s) 230, 360, 440
RsaNI GTAC 3 cut(s) 229, 359, 439
RseI CAYNNNNRTG 2 cut(s) 41, 188
SapI GCTCTTC 1 cut(s) 86
SatI GCNGC 5 cut(s) 63, 77, 98, 218, 407
Sau3AI GATC 1 cut(s) 258
Sau96I GGNCC 1 cut(s) 131
SchI GAGTC 2 cut(s) 284, 412
ScrFI CCNGG 1 cut(s) 112
SfaNI GCATC 1 cut(s) 135
SfcI CTRYAG 1 cut(s) 39
SinI GGWCC 1 cut(s) 131
SmiMI CAYNNNNRTG 2 cut(s) 41, 188
SsiI CCGC 7 cut(s) 50, 97, 124, 178, 263, 326, 407
SspMI CTAG 2 cut(s) 165, 462
StyD4I CCNGG 1 cut(s) 110
TaaI ACNGT 4 cut(s) 40, 228, 379, 385
TaiI ACGT 3 cut(s) 192, 198, 314
TaqI TCGA 2 cut(s) 186, 242
TauI GCSGC 2 cut(s) 100, 409
TscAI CASTG 2 cut(s) 43, 177
TseFI GTSAC 3 cut(s) 52, 373, 379
TseI GCWGC 3 cut(s) 62, 76, 217
Tsp45I GTSAC 3 cut(s) 52, 373, 379
TspDTI ATGAA 2 cut(s) 17, 306
TspGWI ACGGA 1 cut(s) 426
TspRI CASTG 2 cut(s) 43, 177
VpaK11BI GGWCC 1 cut(s) 131
XspI CTAG 2 cut(s) 165, 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.