MD14G1042300.v1.1

Catalyzes the conversion of 7,8-dihydroneopterin to 6- hydroxymethyl-7,8-dihydropterin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007343
Physical Location & Seq
Forward (+)
4017268 .. 4019395
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1042300.v1.1.491

Sequence Viewer

Length: 447 bp
ATGCAAAAGCTGTCGCTAATGGCTGCGCCAATTTCTGGAACAATACAGGAAGATGCTGCAGTAAGAGGGGGAGATAAGCTCATATTGAGGGGTTTGAAGTTCCATGGTTTCCATGGGGTGAAGCCAGAGGAAAGGAAGTTGGGTCAGAAGTTTGTAATAGATGTTGATGCTTGGATGGATCTCCGGGAGGCTGGTAAATCTGATCGTTTGTCAGATACCATTAGTTACACCGAAATCTATCGCATCGTGAAGGAAGTTGTAGAAGGGCCACCTCATAATCTTCTGGAGTCTGTGGCTCAACAAATTGCATCTACCACTCTGAAAAACTATCCCCAGATATCTGCTGTCTCTGTGAAAGTTGGGAAGCCGCATGTTGCTGTTCATGGTTCTCTTGACTACTTGGGGGTTGAGATTTTCAGATACAGAAGTATTGATGCGCCAAAGTGA

Protein Analysis

149

Amino Acids

16.38

Weight (kDa)

8.8

Isoelectric Point (pI)

22.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FolB PF02152 28 - 138 6e-38 Dihydroneopterin aldolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 35
AciI CCGC 1 cut(s) 368
AclWI GGATC 1 cut(s) 186
AfiI CCNNNNNNNGG 1 cut(s) 35
AgsI TTSAA 1 cut(s) 97
AluBI AGCT 2 cut(s) 10, 79
AluI AGCT 2 cut(s) 10, 79
Alw26I GTCTC 1 cut(s) 352
AlwI GGATC 1 cut(s) 186
AoxI GGCC 1 cut(s) 266
ApeKI GCWGC 2 cut(s) 23, 56
AspLEI GCGC 2 cut(s) 28, 439
AspS9I GGNCC 1 cut(s) 266
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 130
BaeI ACNNNNGTAYC 2 cut(s) 412, 445
BbvI GCAGC 2 cut(s) 10, 43
BccI CCATC 1 cut(s) 169
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 352
BfmI CTRYAG 1 cut(s) 57
BisI GCNGC 3 cut(s) 24, 57, 368
BlsI GCNGC 3 cut(s) 25, 58, 369
Bme1390I CCNGG 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 266
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 5 cut(s) 43, 157, 252, 317, 424
BplI GAGNNNNNCTC 2 cut(s) 63, 95
BpmI CTGGAG 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 185
BsaJI CCNNGG 2 cut(s) 103, 112
Bsc4I CCNNNNNNNGG 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 103, 112
BseGI GGATG 1 cut(s) 180
BseLI CCNNNNNNNGG 1 cut(s) 35
BseXI GCAGC 2 cut(s) 10, 43
BshFI GGCC 1 cut(s) 268
BsiSI CCGG 1 cut(s) 184
BslI CCNNNNNNNGG 1 cut(s) 35
BsmAI GTCTC 1 cut(s) 352
BsnI GGCC 1 cut(s) 268
Bsp143I GATC 2 cut(s) 178, 202
Bsp19I CCATGG 2 cut(s) 103, 112
BspACI CCGC 1 cut(s) 368
BspANI GGCC 1 cut(s) 268
BspMAI CTGCAG 1 cut(s) 61
BspPI GGATC 1 cut(s) 186
BssECI CCNNGG 2 cut(s) 103, 112
BssMI GATC 2 cut(s) 178, 202
BssT1I CCWWGG 2 cut(s) 103, 112
BstDSI CCRYGG 2 cut(s) 103, 112
BstF5I GGATG 1 cut(s) 180
BstHHI GCGC 2 cut(s) 28, 439
BstKTI GATC 2 cut(s) 181, 205
BstMAI GTCTC 1 cut(s) 352
BstMBI GATC 2 cut(s) 178, 202
BstNSI RCATGY 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 183
BstSFI CTRYAG 1 cut(s) 57
BstV1I GCAGC 2 cut(s) 10, 43
BstX2I RGATCY 1 cut(s) 178
BstYI RGATCY 1 cut(s) 178
BsuRI GGCC 1 cut(s) 268
BtgI CCRYGG 2 cut(s) 103, 112
BtsCI GGATG 1 cut(s) 180
CfoI GCGC 2 cut(s) 28, 439
Cfr13I GGNCC 1 cut(s) 266
CviAII CATG 4 cut(s) 104, 113, 371, 383
CviJI RGCY 8 cut(s) 10, 23, 79, 124, 191, 268, 296, 367
CviKI_1 RGCY 8 cut(s) 10, 23, 79, 124, 191, 268, 296, 367
DpnI GATC 2 cut(s) 180, 204
DpnII GATC 2 cut(s) 178, 202
Eco130I CCWWGG 2 cut(s) 103, 112
Eco32I GATATC 1 cut(s) 339
EcoRV GATATC 1 cut(s) 339
EcoT14I CCWWGG 2 cut(s) 103, 112
ErhI CCWWGG 2 cut(s) 103, 112
FaeI CATG 4 cut(s) 107, 116, 374, 386
FaiI YATR 6 cut(s) 83, 105, 114, 276, 372, 384
FatI CATG 4 cut(s) 103, 112, 370, 382
Fnu4HI GCNGC 3 cut(s) 24, 57, 368
FokI GGATG 1 cut(s) 187
Fsp4HI GCNGC 3 cut(s) 24, 57, 368
GlaI GCGC 2 cut(s) 27, 438
GluI GCNGC 3 cut(s) 24, 57, 368
GsuI CTGGAG 1 cut(s) 305
HaeIII GGCC 1 cut(s) 268
HapII CCGG 1 cut(s) 184
HhaI GCGC 2 cut(s) 28, 439
Hin1II CATG 4 cut(s) 107, 116, 374, 386
Hin6I GCGC 2 cut(s) 26, 437
HinP1I GCGC 2 cut(s) 26, 437
HinfI GANTC 1 cut(s) 287
HpaII CCGG 1 cut(s) 184
HphI GGTGA 1 cut(s) 130
Hpy188I TCNGA 5 cut(s) 147, 202, 214, 321, 419
Hpy188III TCNNGA 4 cut(s) 36, 247, 284, 392
HpyAV CCTTC 2 cut(s) 244, 257
HpyCH4V TGCA 3 cut(s) 4, 59, 308
Hsp92II CATG 4 cut(s) 107, 116, 374, 386
HspAI GCGC 2 cut(s) 26, 437
Kzo9I GATC 2 cut(s) 178, 202
LpnPI CCDG 7 cut(s) 21, 32, 138, 177, 197, 269, 347
Lsp1109I GCAGC 2 cut(s) 10, 43
LweI GCATC 5 cut(s) 43, 157, 252, 317, 424
MaeIII GTNAC 1 cut(s) 224
MalI GATC 2 cut(s) 180, 204
MboI GATC 2 cut(s) 178, 202
MboII GAAGA 2 cut(s) 62, 272
MflI RGATCY 1 cut(s) 178
MluCI AATT 2 cut(s) 30, 303
MlyI GAGTC 1 cut(s) 296
MnlI CCTC 5 cut(s) 59, 81, 121, 181, 282
MspI CCGG 1 cut(s) 184
MspR9I CCNGG 1 cut(s) 185
NciI CCSGG 1 cut(s) 185
NcoI CCATGG 2 cut(s) 103, 112
NdeII GATC 2 cut(s) 178, 202
NlaIII CATG 4 cut(s) 107, 116, 374, 386
NspI RCATGY 1 cut(s) 374
PflMI CCANNNNNTGG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 183
PkrI GCNGC 3 cut(s) 25, 58, 369
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
PspPI GGNCC 1 cut(s) 266
PstI CTGCAG 1 cut(s) 61
PsuI RGATCY 1 cut(s) 178
SatI GCNGC 3 cut(s) 24, 57, 368
Sau3AI GATC 2 cut(s) 178, 202
Sau96I GGNCC 1 cut(s) 266
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 1 cut(s) 185
SetI ASST 3 cut(s) 12, 81, 274
SfaNI GCATC 5 cut(s) 43, 157, 252, 317, 424
SfcI CTRYAG 1 cut(s) 57
Sse9I AATT 2 cut(s) 30, 303
SsiI CCGC 1 cut(s) 368
StyD4I CCNGG 1 cut(s) 183
StyI CCWWGG 2 cut(s) 103, 112
TasI AATT 2 cut(s) 30, 303
TauI GCSGC 1 cut(s) 370
TseI GCWGC 2 cut(s) 23, 56
TspDTI ATGAA 1 cut(s) 371
Van91I CCANNNNNTGG 1 cut(s) 35
XceI RCATGY 1 cut(s) 374
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.