Rorug03G0155500

Catalyzes the conversion of 7,8-dihydroneopterin to 6- hydroxymethyl-7,8-dihydropterin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007343
Physical Location & Seq
Forward (+)
12812127 .. 12814928
2802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0155500.1

Sequence Viewer

Length: 2592 bp
ATGGCTCGGATAAGAAAGCACGGGGCTAATTTCTCTGGGGAGGGAGGAGTTGAGAGCGTTGATGATAGTGATGCAGTAGTGGGTGAGGAGATGAGTGGGGGTTTGGAAGAGGGAGGTGAATTGGTAGATGGAGGTGGTGATTCTGGAGGTAAAGTAGGACCTGTAGATATGGAGATTGATGAGAAAGGTGGAGATGGAGTGAGAGAGGGTTTGGAGGGGACTCAATTTGGGGGTACTGATGTGAATGCAGCGAGTGAGAAAGGGTCGGTTTCGGAATCGAAGGATGGTTCTAAGGGAGTTGAAAGTTCAGTCTTTAGTGACAAGGATAGGGGTAAAGATGAAATTTTGAGGGAATGCGTGGAAGCCAAAGATGTGGGTGCTGTGGAAGGGAACCTTTCGATTAGTTTCGAGGCGAAGGAGGATGGAACAGTGGAGGGAAAGGATGAATTGATGAGAGATGACAAAGAAGAGGAAGTTGCACAAGTAGATGAGGAAGAGAGAGAAAGAGAACAGAATGTGGAGTGTAGCCAGGTTGGAGAGAAGAGTGCCCATGCTACAAGTTCTAACGTGGAGCATGAGGCTACAGGGTCTGTTGGAGAAATACCGCAGGACATCCCAGCTGTGGAAGTGAAAATTGCTGGTATCGATGAGTCTGGGCATGATGGAGACCTGAATGCAAGCACTGGTTCCCAGATGGTTATGTCTGGTGGACTAGAAAATCAGACGTTGGAAGTTAATGTGGGAGTAAAGACCGGTGAAGATGTGTTGATGCCTGAATATGTTTCCCCAGCAGAGGTTTCTGTGAAATCTGAAAATGGGCATAATTTGATTGACATGGCTATTGATTTGGAACCGTATGCTGGAAAGGATGGGAATGTTTCAGATGATAGTAATGTTTCTGGTCCCTCACAACCAGAATTTCAAGCTTTTGATCTAGTATGGGGCAAAATTAGGAGCCATCCATGGTGGCCTGGGCAGATATGTGATCCTTCAGCTTCATCAAAACTTGCAAAGAAGTATTTTAAGAAGCACAGTTATCTGATAGCTTATTTTTGGGATTGTACATTTGCTTGGAATGAAGCACCACAAATTAAGCCCTTTAGGGAGAACTTTTCACAAATGGAAAAGCAGAGCAATATGGAGGAATTCCAAAATGCAGTTGCTTGTGCTCTGGATGAAGTCTCAAGGCGAGTAGAGTATGGCTTGGCCTGTTCTTGCATTTCCAAAGAAGTTTATGCAAAGATCAAAACACAGACGATCATTAATGCTGGAATCAAGGAACAAGCATGTAGAAGAGATGGTGGTGATAGTTCTCTGGGTGCTGCTTCTTTTGTACCCTTGAAACTCATTAACTTTGCAAAAGAAGCCGCCAAGTTGCCATTTGGCAGAGCTGACAGACTAGAACTTGTTACATCACGGGCCCAATTATCATCTTTCTATCGTTGGAAGGGTTATTCTCAGCTGCCAGAGTTCAATTTTCTTGGTGGGTTGCTGGAAAATGATGCCGACATTTCACTATTGAGAGAAAAGGAGCAATTTAATGAACTACGGGAAGATGCACTTCCAGTAATTAAGGATGAGGAATTGGAGCACAAATCAAAAGGGAAAAGCTCCTCATTGCATAAGCGTAAGCACATATCTGGAGATAGTTCCCAACCCAGTAAAAAAGGGAAAAACTCCTCAAATGTAACTGCTGAGAAGGGTTTGTCTACTCCCATGAGTAAGAATGGATCAGAAAGGAAAGCTGACTGTAAGTTGGTTTCACAATCCTCAAGTAAGAAACGAAAGGCAGTTGATGGAGCTATGTCTGATGACTCTGCGGTGAAACATAGTAAAAGCGCTTCATCAGCAGGGGAAGATTATGTTTCTTTACAGAATAAGCAGACTTTTAGAGTTGGAGATAGAATCTGTAGGGTTGCAACCCTACTAAATGGGTCAAGTCCAATATTCAGGCACAATGCAGCACCTGCAGATGCTGAAGTTCAGGATGAAGGCAAAGAGAAATCTACATCGCAGAAACCACAAACTGAAAAGCTGATTGACATGGAGGATCCTTCTCCTGATGAGATGCTGTCTCAGCTTTGCTTGGCTGCTATGAATCCCAATAAAGGGTACAGCATGTTGATTTCCTTGATGGGTTGCTTCTCAAAATTTAGGAATACTATATGCCTGGAACCTCCCAGTCCAGATGAGGATCAATTGCCTTTGGGACAAATATTTGGTGGCAAGCACAGTAAGAAATTAACCAAGACTGGGAAGAAATCAATCAAACCACGCATCTCTGAAAAATCTGAGTTGGAGCTGGTCAAAGACTCTTATTCTTCTGATAAGATAGTTAAAAGTCAAAGTGAAAATGGAGAGTTATTGCTTGGAGCTGCAAGCGATAAAGATACATGTGTTGTGGAGTCACAATCAAGTCCTGATTCGGAAGCAGTGAAGCCAGTTCTTGACAATAAAAATGGGGAATTGGTGTGTGGAACTGCTACAAGCGATAAGGATACTTCTACTGTTGAACTGCAAGCAAGTCTTGAGTTAAATCCCACTTTGGATTCTGAACAGAACATTGTGGAGATATTGAATCAAAAGCAGTGA

Protein Analysis

863

Amino Acids

93.39

Weight (kDa)

4.83

Isoelectric Point (pI)

44.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 311 - 393 1.5e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1975
Acc36I ACCTGC 1 cut(s) 1975
AccI GTMKAC 1 cut(s) 1709
AciI CCGC 3 cut(s) 605, 1368, 1820
AclWI GGATC 5 cut(s) 980, 1738, 2045, 2058, 2202
AcsI RAATTY 4 cut(s) 342, 917, 1145, 2150
AcuI CTGAAG 2 cut(s) 975, 1998
AfaI GTAC 4 cut(s) 235, 1063, 1335, 2114
AfeI AGCGCT 1 cut(s) 1840
AfiI CCNNNNNNNGG 6 cut(s) 622, 793, 860, 2108, 2109, 2253
AflIII ACRYGT 1 cut(s) 2393
AgeI ACCGGT 1 cut(s) 752
AgsI TTSAA 6 cut(s) 302, 923, 1342, 1474, 2513, 2578
AjnI CCWGG 3 cut(s) 528, 970, 2169
Alw21I GWGCWC 2 cut(s) 1171, 1593
Alw26I GTCTC 3 cut(s) 660, 1186, 2079
AlwI GGATC 5 cut(s) 980, 1738, 2045, 2058, 2202
AlwNI CAGNNNCTG 3 cut(s) 590, 1967, 1976
Aor51HI AGCGCT 1 cut(s) 1840
AoxI GGCC 3 cut(s) 968, 1206, 1419
ApaI GGGCCC 1 cut(s) 1423
ApeKI GCWGC 6 cut(s) 248, 1322, 1462, 1961, 2090, 2375
ApoI RAATTY 4 cut(s) 342, 917, 1145, 2150
AseI ATTAAT 1 cut(s) 1263
AsiGI ACCGGT 1 cut(s) 752
AspLEI GCGC 1 cut(s) 1841
AspS9I GGNCC 4 cut(s) 158, 902, 1419, 1420
AsuHPI GGTGA 6 cut(s) 95, 128, 149, 767, 1316, 1834
AvaII GGWCC 2 cut(s) 158, 902
BaeGI GKGCMC 2 cut(s) 550, 1423
BaeI ACNNNNGTAYC 4 cut(s) 225, 258, 2382, 2415
BamHI GGATCC 1 cut(s) 2050
BanII GRGCYC 1 cut(s) 1423
BarI GAAGNNNNNNTAC 2 cut(s) 1825, 1857
Bbv12I GWGCWC 2 cut(s) 1171, 1593
BbvI GCAGC 6 cut(s) 260, 1309, 1449, 1973, 2077, 2362
BciT130I CCWGG 3 cut(s) 530, 972, 2171
BciVI GTATCC 1 cut(s) 2491
BcoDI GTCTC 3 cut(s) 660, 1186, 2079
BfaI CTAG 3 cut(s) 713, 935, 1400
BfmI CTRYAG 4 cut(s) 162, 582, 1909, 1968
BfoI RGCGCY 1 cut(s) 1842
BfuAI ACCTGC 1 cut(s) 1975
BfuI GTATCC 1 cut(s) 2491
BisI GCNGC 7 cut(s) 249, 1323, 1368, 1463, 1962, 2091, 2376
BlsI GCNGC 7 cut(s) 250, 1324, 1369, 1464, 1963, 2092, 2377
Bme1390I CCNGG 3 cut(s) 530, 972, 2171
Bme18I GGWCC 2 cut(s) 158, 902
BmgT120I GGNCC 4 cut(s) 158, 902, 1419, 1420
BmiI GGNNCC 8 cut(s) 392, 688, 852, 904, 956, 1421, 2052, 2175
BmrFI CCNGG 3 cut(s) 530, 972, 2171
BmrI ACTGGG 3 cut(s) 1653, 2175, 2262
BmsI GCATC 7 cut(s) 61, 759, 1492, 1546, 1963, 2058, 2286
BmuI ACTGGG 3 cut(s) 1653, 2175, 2262
BpmI CTGGAG 2 cut(s) 165, 1662
BpuEI CTTGAG 3 cut(s) 1168, 1756, 2549
Bsa29I ATCGAT 1 cut(s) 645
BsaBI GATNNNNATC 1 cut(s) 2193
BsaI GGTCTC 1 cut(s) 660
BsaJI CCNNGG 2 cut(s) 962, 971
BsaWI WCCGGW 1 cut(s) 752
BsaXI ACNNNNNCTCC 4 cut(s) 138, 168, 183, 213
Bsc4I CCNNNNNNNGG 6 cut(s) 622, 793, 860, 2108, 2109, 2253
Bse118I RCCGGY 1 cut(s) 752
Bse1I ACTGG 6 cut(s) 688, 1565, 1659, 2181, 2257, 2441
Bse3DI GCAATG 1 cut(s) 1616
Bse8I GATNNNNATC 1 cut(s) 2193
BseBI CCWGG 3 cut(s) 530, 972, 2171
BseCI ATCGAT 1 cut(s) 645
BseDI CCNNGG 2 cut(s) 962, 971
BseGI GGATG 9 cut(s) 289, 427, 448, 612, 874, 958, 1180, 1582, 1993
BseJI GATNNNNATC 1 cut(s) 2193
BseLI CCNNNNNNNGG 6 cut(s) 622, 793, 860, 2108, 2109, 2253
BseMI GCAATG 1 cut(s) 1616
BseMII CTCAG 4 cut(s) 1472, 1686, 2090, 2283
BseNI ACTGG 6 cut(s) 688, 1565, 1659, 2181, 2257, 2441
BseRI GAGGAG 4 cut(s) 60, 101, 1603, 1669
BseSI GKGCMC 2 cut(s) 550, 1423
BseXI GCAGC 6 cut(s) 260, 1309, 1449, 1973, 2077, 2362
BseYI CCCAGC 2 cut(s) 616, 787
BshFI GGCC 3 cut(s) 970, 1208, 1421
BshTI ACCGGT 1 cut(s) 752
BshVI ATCGAT 1 cut(s) 645
BsiHKAI GWGCWC 2 cut(s) 1171, 1593
BsiSI CCGG 1 cut(s) 753
BslFI GGGAC 3 cut(s) 232, 888, 2223
BslI CCNNNNNNNGG 6 cut(s) 622, 793, 860, 2108, 2109, 2253
BsmAI GTCTC 3 cut(s) 660, 1186, 2079
BsmFI GGGAC 3 cut(s) 232, 888, 2223
BsmI GAATGC 3 cut(s) 250, 359, 679
BsnI GGCC 3 cut(s) 970, 1208, 1421
Bso31I GGTCTC 1 cut(s) 660
Bsp120I GGGCCC 1 cut(s) 1419
Bsp1286I GDGCHC 4 cut(s) 550, 1171, 1423, 1593
Bsp1407I TGTACA 1 cut(s) 1061
Bsp143I GATC 7 cut(s) 931, 985, 1242, 1257, 1730, 2050, 2194
Bsp19I CCATGG 1 cut(s) 962
BspACI CCGC 3 cut(s) 605, 1368, 1820
BspANI GGCC 3 cut(s) 970, 1208, 1421
BspCNI CTCAG 4 cut(s) 1471, 1687, 2089, 2284
BspDI ATCGAT 1 cut(s) 645
BspLI GGNNCC 8 cut(s) 392, 688, 852, 904, 956, 1421, 2052, 2175
BspMAI CTGCAG 1 cut(s) 1972
BspMI ACCTGC 1 cut(s) 1975
BspPI GGATC 5 cut(s) 980, 1738, 2045, 2058, 2202
BspTNI GGTCTC 1 cut(s) 660
BsrDI GCAATG 1 cut(s) 1616
BsrFI RCCGGY 1 cut(s) 752
BsrGI TGTACA 1 cut(s) 1061
BsrI ACTGG 6 cut(s) 688, 1565, 1659, 2181, 2257, 2441
BssAI RCCGGY 1 cut(s) 752
BssECI CCNNGG 2 cut(s) 962, 971
BssMI GATC 7 cut(s) 931, 985, 1242, 1257, 1730, 2050, 2194
BssT1I CCWWGG 1 cut(s) 962
Bst2UI CCWGG 3 cut(s) 530, 972, 2171
Bst4CI ACNGT 6 cut(s) 430, 855, 1034, 1751, 2234, 2509
Bst6I CTCTTC 5 cut(s) 102, 462, 489, 536, 1288
BstAPI GCANNNNNTGC 1 cut(s) 1967
BstAUI TGTACA 1 cut(s) 1061
BstC8I GCNNGC 4 cut(s) 679, 2228, 2380, 2520
BstDEI CTNAG 5 cut(s) 291, 1458, 1695, 2076, 2292
BstDSI CCRYGG 1 cut(s) 962
BstF5I GGATG 9 cut(s) 289, 427, 448, 612, 874, 958, 1180, 1582, 1993
BstH2I RGCGCY 1 cut(s) 1842
BstHHI GCGC 1 cut(s) 1841
BstKTI GATC 7 cut(s) 934, 988, 1245, 1260, 1733, 2053, 2197
BstMAI GTCTC 3 cut(s) 660, 1186, 2079
BstMBI GATC 7 cut(s) 931, 985, 1242, 1257, 1730, 2050, 2194
BstMWI GCNNNNNNNGC 4 cut(s) 1364, 1847, 1967, 2077
BstNI CCWGG 3 cut(s) 530, 972, 2171
BstNSI RCATGY 3 cut(s) 1290, 2122, 2397
BstSCI CCNGG 3 cut(s) 528, 970, 2169
BstSFI CTRYAG 4 cut(s) 162, 582, 1909, 1968
BstSLI GKGCMC 2 cut(s) 550, 1423
BstV1I GCAGC 6 cut(s) 260, 1309, 1449, 1973, 2077, 2362
BstX2I RGATCY 1 cut(s) 2050
BstXI CCANNNNNNTGG 1 cut(s) 373
BstYI RGATCY 1 cut(s) 2050
Bsu15I ATCGAT 1 cut(s) 645
BsuI GTATCC 1 cut(s) 2491
BsuRI GGCC 3 cut(s) 970, 1208, 1421
BsuTUI ATCGAT 1 cut(s) 645
BtgI CCRYGG 1 cut(s) 962
BtgZI GCGATG 1 cut(s) 1995
BtsCI GGATG 9 cut(s) 289, 427, 448, 612, 874, 958, 1180, 1582, 1993
BtsI GCAGTG 1 cut(s) 2439
BtsIMutI CAGTG 3 cut(s) 435, 681, 2439
BveI ACCTGC 1 cut(s) 1975
Cac8I GCNNGC 4 cut(s) 679, 2228, 2380, 2520
CaiI CAGNNNCTG 3 cut(s) 590, 1967, 1976
CfoI GCGC 1 cut(s) 1841
Cfr10I RCCGGY 1 cut(s) 752
Cfr13I GGNCC 4 cut(s) 158, 902, 1419, 1420
ClaI ATCGAT 1 cut(s) 645
Csp6I GTAC 4 cut(s) 234, 1062, 1334, 2113
CspAI ACCGGT 1 cut(s) 752
CviQI GTAC 4 cut(s) 234, 1062, 1334, 2113
DdeI CTNAG 5 cut(s) 291, 1458, 1695, 2076, 2292
DpnI GATC 7 cut(s) 933, 987, 1244, 1259, 1732, 2052, 2196
DpnII GATC 7 cut(s) 931, 985, 1242, 1257, 1730, 2050, 2194
Eam1104I CTCTTC 5 cut(s) 102, 462, 489, 536, 1288
EarI CTCTTC 5 cut(s) 102, 462, 489, 536, 1288
Eco130I CCWWGG 1 cut(s) 962
Eco24I GRGCYC 1 cut(s) 1423
Eco31I GGTCTC 1 cut(s) 660
Eco47I GGWCC 2 cut(s) 158, 902
Eco47III AGCGCT 1 cut(s) 1840
Eco57I CTGAAG 2 cut(s) 975, 1998
EcoO109I RGGNCCY 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 1145
EcoRII CCWGG 3 cut(s) 528, 970, 2169
EcoT14I CCWWGG 1 cut(s) 962
EcoT38I GRGCYC 1 cut(s) 1423
ErhI CCWWGG 1 cut(s) 962
FalI AAGNNNNNCTT 6 cut(s) 378, 410, 1545, 1577, 2511, 2543
FaqI GGGAC 3 cut(s) 232, 888, 2223
FblI GTMKAC 1 cut(s) 1709
Fnu4HI GCNGC 7 cut(s) 249, 1323, 1368, 1463, 1962, 2091, 2376
FokI GGATG 9 cut(s) 296, 434, 455, 599, 881, 945, 1187, 1589, 2000
FriOI GRGCYC 1 cut(s) 1423
Fsp4HI GCNGC 7 cut(s) 249, 1323, 1368, 1463, 1962, 2091, 2376
FspBI CTAG 3 cut(s) 713, 935, 1400
GlaI GCGC 1 cut(s) 1840
GluI GCNGC 7 cut(s) 249, 1323, 1368, 1463, 1962, 2091, 2376
GsaI CCCAGC 2 cut(s) 620, 791
GsuI CTGGAG 2 cut(s) 165, 1662
HaeII RGCGCY 1 cut(s) 1842
HaeIII GGCC 3 cut(s) 970, 1208, 1421
HapII CCGG 1 cut(s) 753
HhaI GCGC 1 cut(s) 1841
Hin6I GCGC 1 cut(s) 1839
HinP1I GCGC 1 cut(s) 1839
HindIII AAGCTT 1 cut(s) 924
HpaII CCGG 1 cut(s) 753
HphI GGTGA 6 cut(s) 95, 128, 149, 767, 1316, 1834
Hpy166II GTNNAC 2 cut(s) 710, 1710
Hpy188III TCNNGA 9 cut(s) 144, 1172, 1641, 1985, 2060, 2186, 2420, 2447, 2528
Hpy8I GTNNAC 2 cut(s) 710, 1710
HpyAV CCTTC 8 cut(s) 274, 380, 409, 999, 1441, 1693, 1985, 2064
HpyCH4III ACNGT 6 cut(s) 430, 855, 1034, 1751, 2234, 2509
HpyCH4IV ACGT 2 cut(s) 567, 725
HpyF10VI GCNNNNNNNGC 4 cut(s) 1364, 1847, 1967, 2077
HpyF3I CTNAG 5 cut(s) 291, 1458, 1695, 2076, 2292
HpySE526I ACGT 2 cut(s) 567, 725
HspAI GCGC 1 cut(s) 1839
Kzo9I GATC 7 cut(s) 931, 985, 1242, 1257, 1730, 2050, 2194
LmnI GCTCC 8 cut(s) 571, 954, 1531, 1588, 1616, 1799, 2299, 2372
Lsp1109I GCAGC 6 cut(s) 260, 1309, 1449, 1973, 2077, 2362
LweI GCATC 7 cut(s) 61, 759, 1492, 1546, 1963, 2058, 2286
MaeI CTAG 3 cut(s) 713, 935, 1400
MaeII ACGT 2 cut(s) 567, 725
MaeIII GTNAC 4 cut(s) 317, 1408, 1687, 2406
MalI GATC 7 cut(s) 933, 987, 1244, 1259, 1732, 2052, 2196
MboI GATC 7 cut(s) 931, 985, 1242, 1257, 1730, 2050, 2194
MfeI CAATTG 1 cut(s) 2198
MflI RGATCY 1 cut(s) 2050
MhlI GDGCHC 4 cut(s) 550, 1171, 1423, 1593
MlyI GAGTC 5 cut(s) 214, 659, 1808, 2306, 2414
MmeI TCCRAC 6 cut(s) 514, 574, 708, 1424, 1876, 2277
MspA1I CMGCKG 2 cut(s) 620, 1462
MspI CCGG 1 cut(s) 753
MspR9I CCNGG 3 cut(s) 530, 972, 2171
MunI CAATTG 1 cut(s) 2198
Mva1269I GAATGC 3 cut(s) 250, 359, 679
MvaI CCWGG 3 cut(s) 530, 972, 2171
MwoI GCNNNNNNNGC 4 cut(s) 1364, 1847, 1967, 2077
NcoI CCATGG 1 cut(s) 962
NdeII GATC 7 cut(s) 931, 985, 1242, 1257, 1730, 2050, 2194
NlaIV GGNNCC 8 cut(s) 392, 688, 852, 904, 956, 1421, 2052, 2175
NmuCI GTSAC 2 cut(s) 317, 2406
NspI RCATGY 3 cut(s) 1290, 2122, 2397
PaqCI CACCTGC 1 cut(s) 1975
PciI ACATGT 1 cut(s) 2393
PctI GAATGC 3 cut(s) 250, 359, 679
PfeI GAWTC 8 cut(s) 140, 275, 1272, 1905, 2098, 2423, 2549, 2578
PinAI ACCGGT 1 cut(s) 752
PkrI GCNGC 7 cut(s) 250, 1324, 1369, 1464, 1963, 2092, 2377
PleI GAGTC 5 cut(s) 214, 658, 1808, 2306, 2413
PpsI GAGTC 5 cut(s) 214, 658, 1808, 2306, 2413
PpuMI RGGWCCY 1 cut(s) 158
PscI ACATGT 1 cut(s) 2393
PshBI ATTAAT 1 cut(s) 1263
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 3 cut(s) 528, 970, 2169
PspFI CCCAGC 2 cut(s) 616, 787
PspGI CCWGG 3 cut(s) 528, 970, 2169
PspN4I GGNNCC 8 cut(s) 392, 688, 852, 904, 956, 1421, 2052, 2175
PspOMI GGGCCC 1 cut(s) 1419
PspPI GGNCC 4 cut(s) 158, 902, 1419, 1420
PspPPI RGGWCCY 1 cut(s) 158
PstI CTGCAG 1 cut(s) 1972
PstNI CAGNNNCTG 3 cut(s) 590, 1967, 1976
PsuI RGATCY 1 cut(s) 2050
PvuII CAGCTG 2 cut(s) 620, 1462
RsaI GTAC 4 cut(s) 235, 1063, 1335, 2114
RsaNI GTAC 4 cut(s) 234, 1062, 1334, 2113
SatI GCNGC 7 cut(s) 249, 1323, 1368, 1463, 1962, 2091, 2376
Sau3AI GATC 7 cut(s) 931, 985, 1242, 1257, 1730, 2050, 2194
Sau96I GGNCC 4 cut(s) 158, 902, 1419, 1420
SchI GAGTC 5 cut(s) 214, 659, 1808, 2306, 2414
ScrFI CCNGG 3 cut(s) 530, 972, 2171
SduI GDGCHC 4 cut(s) 550, 1171, 1423, 1593
SfaNI GCATC 7 cut(s) 61, 759, 1492, 1546, 1963, 2058, 2286
SfcI CTRYAG 4 cut(s) 162, 582, 1909, 1968
SinI GGWCC 2 cut(s) 158, 902
SmlI CTYRAG 3 cut(s) 1183, 1771, 2528
SmoI CTYRAG 3 cut(s) 1183, 1771, 2528
SsiI CCGC 3 cut(s) 605, 1368, 1820
SspI AATATT 2 cut(s) 1947, 2217
SspMI CTAG 3 cut(s) 713, 935, 1400
StyD4I CCNGG 3 cut(s) 528, 970, 2169
StyI CCWWGG 1 cut(s) 962
TaaI ACNGT 6 cut(s) 430, 855, 1034, 1751, 2234, 2509
TaiI ACGT 2 cut(s) 570, 728
TaqI TCGA 4 cut(s) 278, 398, 408, 645
TatI WGTACW 1 cut(s) 1061
TauI GCSGC 1 cut(s) 1370
TfiI GAWTC 8 cut(s) 140, 275, 1272, 1905, 2098, 2423, 2549, 2578
TscAI CASTG 3 cut(s) 435, 688, 2439
TseFI GTSAC 2 cut(s) 317, 2406
TseI GCWGC 6 cut(s) 248, 1322, 1462, 1961, 2090, 2375
Tsp45I GTSAC 2 cut(s) 317, 2406
TspDTI ATGAA 9 cut(s) 354, 459, 987, 1092, 1191, 1557, 1833, 2004, 2111
TspRI CASTG 3 cut(s) 435, 688, 2439
VpaK11BI GGWCC 2 cut(s) 158, 902
VspI ATTAAT 1 cut(s) 1263
XapI RAATTY 4 cut(s) 342, 917, 1145, 2150
XceI RCATGY 3 cut(s) 1290, 2122, 2397
XmiI GTMKAC 1 cut(s) 1709
XspI CTAG 3 cut(s) 713, 935, 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.