pycom14g03600

Catalyzes the conversion of 7,8-dihydroneopterin to 6- hydroxymethyl-7,8-dihydropterin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007343
Physical Location & Seq
Forward (+)
3032486 .. 3034127
1642 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g03600.1

Sequence Viewer

Length: 429 bp
ATGGCTGCGCCAATTTCTGGAACAATACAGGAAGATGCTGCACTAAGAGGGGGAGATAAGCTCATATTGAGGGGTTTGAAGTTCCATGGTTTCCATGGGGTGAAGCCAGAGGAAAGGAAGTTGGGTCAGAAGTTTGTAATAGATGTTGATGCCTGGATGGATCTCCGGGAGGCTGGTAAATCTGATCGTTTGTCAGATACCATTAGTTACACCGAAATCTATCGCATCGTGAAGGAAGTTGTAGAAGGGCCACCTCATAATCTTCTGGAGTCTGTGGCTCAACAAATTGCATCTACCACTCTGAAAAAGTATCCCCAGATATCTGCTGTCTCTGTGAAAGTTGGGAAGCCGCATGTTGCTGTTCATGGTTCTCTTGACTACTTGGGGGTTGAGATTTTCAGATACAGAAGTATTGATGCGCCAAAGTGA

Protein Analysis

143

Amino Acids

15.71

Weight (kDa)

8.8

Isoelectric Point (pI)

25.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FolB PF02152 22 - 132 5.1e-38 Dihydroneopterin aldolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 17
AciI CCGC 1 cut(s) 350
AclWI GGATC 1 cut(s) 168
AfiI CCNNNNNNNGG 1 cut(s) 17
AgsI TTSAA 1 cut(s) 79
AjnI CCWGG 1 cut(s) 152
AluBI AGCT 1 cut(s) 61
AluI AGCT 1 cut(s) 61
Alw26I GTCTC 1 cut(s) 334
AlwI GGATC 1 cut(s) 168
AoxI GGCC 1 cut(s) 248
ApeKI GCWGC 2 cut(s) 5, 38
AspLEI GCGC 2 cut(s) 10, 421
AspS9I GGNCC 1 cut(s) 248
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 1 cut(s) 112
BaeI ACNNNNGTAYC 2 cut(s) 394, 427
BbvI GCAGC 1 cut(s) 25
BccI CCATC 1 cut(s) 151
BciT130I CCWGG 1 cut(s) 154
BciVI GTATCC 1 cut(s) 321
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 334
BfuI GTATCC 1 cut(s) 321
BisI GCNGC 3 cut(s) 6, 39, 350
BlsI GCNGC 3 cut(s) 7, 40, 351
Bme1390I CCNGG 2 cut(s) 154, 167
BmgT120I GGNCC 1 cut(s) 248
BmrFI CCNGG 2 cut(s) 154, 167
BmsI GCATC 5 cut(s) 25, 139, 234, 299, 406
BplI GAGNNNNNCTC 2 cut(s) 45, 77
BpmI CTGGAG 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 167
BsaJI CCNNGG 2 cut(s) 85, 94
Bsc4I CCNNNNNNNGG 1 cut(s) 17
BseBI CCWGG 1 cut(s) 154
BseDI CCNNGG 2 cut(s) 85, 94
BseGI GGATG 1 cut(s) 162
BseLI CCNNNNNNNGG 1 cut(s) 17
BseXI GCAGC 1 cut(s) 25
BsgI GTGCAG 1 cut(s) 24
BshFI GGCC 1 cut(s) 250
BsiSI CCGG 1 cut(s) 166
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 334
BsnI GGCC 1 cut(s) 250
Bsp143I GATC 2 cut(s) 160, 184
Bsp19I CCATGG 2 cut(s) 85, 94
BspACI CCGC 1 cut(s) 350
BspANI GGCC 1 cut(s) 250
BspPI GGATC 1 cut(s) 168
BssECI CCNNGG 2 cut(s) 85, 94
BssMI GATC 2 cut(s) 160, 184
BssT1I CCWWGG 2 cut(s) 85, 94
Bst2UI CCWGG 1 cut(s) 154
BstDEI CTNAG 1 cut(s) 44
BstDSI CCRYGG 2 cut(s) 85, 94
BstF5I GGATG 1 cut(s) 162
BstHHI GCGC 2 cut(s) 10, 421
BstKTI GATC 2 cut(s) 163, 187
BstMAI GTCTC 1 cut(s) 334
BstMBI GATC 2 cut(s) 160, 184
BstNI CCWGG 1 cut(s) 154
BstNSI RCATGY 1 cut(s) 356
BstSCI CCNGG 2 cut(s) 152, 165
BstV1I GCAGC 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
BsuI GTATCC 1 cut(s) 321
BsuRI GGCC 1 cut(s) 250
BtgI CCRYGG 2 cut(s) 85, 94
BtsCI GGATG 1 cut(s) 162
CfoI GCGC 2 cut(s) 10, 421
Cfr13I GGNCC 1 cut(s) 248
CviAII CATG 4 cut(s) 86, 95, 353, 365
CviJI RGCY 7 cut(s) 5, 61, 106, 173, 250, 278, 349
CviKI_1 RGCY 7 cut(s) 5, 61, 106, 173, 250, 278, 349
DdeI CTNAG 1 cut(s) 44
DpnI GATC 2 cut(s) 162, 186
DpnII GATC 2 cut(s) 160, 184
Eco130I CCWWGG 2 cut(s) 85, 94
Eco32I GATATC 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 152
EcoRV GATATC 1 cut(s) 321
EcoT14I CCWWGG 2 cut(s) 85, 94
ErhI CCWWGG 2 cut(s) 85, 94
FaeI CATG 4 cut(s) 89, 98, 356, 368
FaiI YATR 6 cut(s) 65, 87, 96, 258, 354, 366
FatI CATG 4 cut(s) 85, 94, 352, 364
Fnu4HI GCNGC 3 cut(s) 6, 39, 350
FokI GGATG 1 cut(s) 169
Fsp4HI GCNGC 3 cut(s) 6, 39, 350
GlaI GCGC 2 cut(s) 9, 420
GluI GCNGC 3 cut(s) 6, 39, 350
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 1 cut(s) 250
HapII CCGG 1 cut(s) 166
HhaI GCGC 2 cut(s) 10, 421
Hin1II CATG 4 cut(s) 89, 98, 356, 368
Hin6I GCGC 2 cut(s) 8, 419
HinP1I GCGC 2 cut(s) 8, 419
HinfI GANTC 1 cut(s) 269
HpaII CCGG 1 cut(s) 166
HphI GGTGA 1 cut(s) 112
Hpy188I TCNGA 5 cut(s) 129, 184, 196, 303, 401
Hpy188III TCNNGA 4 cut(s) 18, 229, 266, 374
HpyAV CCTTC 2 cut(s) 226, 239
HpyCH4V TGCA 2 cut(s) 41, 290
HpyF3I CTNAG 1 cut(s) 44
Hsp92II CATG 4 cut(s) 89, 98, 356, 368
HspAI GCGC 2 cut(s) 8, 419
Kzo9I GATC 2 cut(s) 160, 184
LpnPI CCDG 9 cut(s) 3, 14, 120, 139, 159, 166, 179, 251, 329
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 5 cut(s) 25, 139, 234, 299, 406
MaeIII GTNAC 1 cut(s) 206
MalI GATC 2 cut(s) 162, 186
MboI GATC 2 cut(s) 160, 184
MboII GAAGA 2 cut(s) 44, 254
MflI RGATCY 1 cut(s) 160
MluCI AATT 2 cut(s) 12, 285
MlyI GAGTC 1 cut(s) 278
MnlI CCTC 5 cut(s) 41, 63, 103, 163, 264
MspI CCGG 1 cut(s) 166
MspR9I CCNGG 2 cut(s) 154, 167
MvaI CCWGG 1 cut(s) 154
NciI CCSGG 1 cut(s) 167
NcoI CCATGG 2 cut(s) 85, 94
NdeII GATC 2 cut(s) 160, 184
NlaIII CATG 4 cut(s) 89, 98, 356, 368
NspI RCATGY 1 cut(s) 356
PflMI CCANNNNNTGG 1 cut(s) 17
PfoI TCCNGGA 1 cut(s) 165
PkrI GCNGC 3 cut(s) 7, 40, 351
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
Psp6I CCWGG 1 cut(s) 152
PspGI CCWGG 1 cut(s) 152
PspPI GGNCC 1 cut(s) 248
PsuI RGATCY 1 cut(s) 160
SatI GCNGC 3 cut(s) 6, 39, 350
Sau3AI GATC 2 cut(s) 160, 184
Sau96I GGNCC 1 cut(s) 248
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 2 cut(s) 154, 167
SetI ASST 2 cut(s) 63, 256
SfaNI GCATC 5 cut(s) 25, 139, 234, 299, 406
Sse9I AATT 2 cut(s) 12, 285
SsiI CCGC 1 cut(s) 350
StyD4I CCNGG 2 cut(s) 152, 165
StyI CCWWGG 2 cut(s) 85, 94
TasI AATT 2 cut(s) 12, 285
TauI GCSGC 1 cut(s) 352
TseI GCWGC 2 cut(s) 5, 38
TspDTI ATGAA 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 17
XceI RCATGY 1 cut(s) 356
XcmI CCANNNNNNNNNTGG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.