Rw3G018770

Catalyzes the conversion of 7,8-dihydroneopterin to 6- hydroxymethyl-7,8-dihydropterin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0007343
Physical Location & Seq
Forward (+)
21063009 .. 21064218
1210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G018770.1

Sequence Viewer

Length: 429 bp
ATGGCTGCTTCTGTGACTGGAACAGCACATGAAGATGCTGCAATTAGAGGGGGAGATAAGCTTGTACTAAGGGGTTTGAAGTTCCATGGTTTCCATGGGGTGAAGCCAGAAGAAAGGAAGTTGGGTCAGAAGTTTCTGATAGATGTTGATGCTTGGATGGATCTTCGGGTGGCCGGAAGATCTGATCAGTTGTCAGACACCATTAGCTACACCGAAATTTATCGCATTGTAAAAGAGGTTGTAGAAGGGCCACCTCATAATCTTCTGGAGTCTGTTGCTGAACTCATTGCATCTACAACTCTGAAAAACTATCCCCAGATATCTGCTGTCTCTGTGAAAGTTGGAAAGCCTCACGTTGCTGTTCATGGTCCTCTTGATTACTTGGGTGTTGAGATATTTAGATACAGAAGTATTGATGCACCGAACTAA

Protein Analysis

142

Amino Acids

15.61

Weight (kDa)

6.59

Isoelectric Point (pI)

20.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FolB PF02152 22 - 132 8.4e-37 Dihydroneopterin aldolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 168
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 216
AfaI GTAC 1 cut(s) 66
AgsI TTSAA 1 cut(s) 79
AluBI AGCT 2 cut(s) 61, 207
AluI AGCT 2 cut(s) 61, 207
Alw26I GTCTC 1 cut(s) 334
AlwI GGATC 1 cut(s) 168
AoxI GGCC 2 cut(s) 171, 248
ApeKI GCWGC 2 cut(s) 5, 38
ApoI RAATTY 1 cut(s) 216
AspS9I GGNCC 2 cut(s) 248, 368
AsuHPI GGTGA 1 cut(s) 112
AvaII GGWCC 1 cut(s) 368
BaeI ACNNNNGTAYC 2 cut(s) 394, 427
BbvI GCAGC 1 cut(s) 25
BccI CCATC 1 cut(s) 151
BclI TGATCA 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 334
BglII AGATCT 1 cut(s) 179
BisI GCNGC 2 cut(s) 6, 39
BlsI GCNGC 2 cut(s) 7, 40
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 2 cut(s) 248, 368
BmsI GCATC 4 cut(s) 25, 139, 299, 406
BpmI CTGGAG 1 cut(s) 287
BsaJI CCNNGG 2 cut(s) 85, 94
Bse1I ACTGG 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 285
BseDI CCNNGG 2 cut(s) 85, 94
BseGI GGATG 1 cut(s) 162
BseMI GCAATG 1 cut(s) 285
BseNI ACTGG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 25
BshFI GGCC 2 cut(s) 173, 250
BsiSI CCGG 1 cut(s) 174
BsmAI GTCTC 1 cut(s) 334
BsnI GGCC 2 cut(s) 173, 250
Bsp143I GATC 3 cut(s) 160, 179, 184
Bsp19I CCATGG 2 cut(s) 85, 94
BspANI GGCC 2 cut(s) 173, 250
BspPI GGATC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 285
BsrI ACTGG 1 cut(s) 22
BssECI CCNNGG 2 cut(s) 85, 94
BssMI GATC 3 cut(s) 160, 179, 184
BssT1I CCWWGG 2 cut(s) 85, 94
BstDEI CTNAG 1 cut(s) 68
BstDSI CCRYGG 2 cut(s) 85, 94
BstF5I GGATG 1 cut(s) 162
BstKTI GATC 3 cut(s) 163, 182, 187
BstMAI GTCTC 1 cut(s) 334
BstMBI GATC 3 cut(s) 160, 179, 184
BstV1I GCAGC 1 cut(s) 25
BstX2I RGATCY 2 cut(s) 160, 179
BstYI RGATCY 2 cut(s) 160, 179
BsuRI GGCC 2 cut(s) 173, 250
BtgI CCRYGG 2 cut(s) 85, 94
BtsCI GGATG 1 cut(s) 162
Cfr13I GGNCC 2 cut(s) 248, 368
Csp6I GTAC 1 cut(s) 65
CviAII CATG 4 cut(s) 29, 86, 95, 365
CviJI RGCY 7 cut(s) 5, 61, 106, 173, 207, 250, 349
CviKI_1 RGCY 7 cut(s) 5, 61, 106, 173, 207, 250, 349
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 1 cut(s) 68
DpnI GATC 3 cut(s) 162, 181, 186
DpnII GATC 3 cut(s) 160, 179, 184
EaeI YGGCCR 1 cut(s) 171
Eco130I CCWWGG 2 cut(s) 85, 94
Eco32I GATATC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 368
EcoRV GATATC 1 cut(s) 321
EcoT14I CCWWGG 2 cut(s) 85, 94
ErhI CCWWGG 2 cut(s) 85, 94
FaeI CATG 4 cut(s) 32, 89, 98, 368
FaiI YATR 5 cut(s) 30, 87, 96, 258, 366
FatI CATG 4 cut(s) 28, 85, 94, 364
FbaI TGATCA 1 cut(s) 184
Fnu4HI GCNGC 2 cut(s) 6, 39
FokI GGATG 1 cut(s) 169
Fsp4HI GCNGC 2 cut(s) 6, 39
GluI GCNGC 2 cut(s) 6, 39
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 2 cut(s) 173, 250
HapII CCGG 1 cut(s) 174
Hin1II CATG 4 cut(s) 32, 89, 98, 368
HindIII AAGCTT 1 cut(s) 59
HinfI GANTC 1 cut(s) 269
HpaII CCGG 1 cut(s) 174
HphI GGTGA 1 cut(s) 112
Hpy188I TCNGA 5 cut(s) 129, 138, 184, 196, 303
Hpy188III TCNNGA 2 cut(s) 266, 374
HpyAV CCTTC 1 cut(s) 239
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 3 cut(s) 41, 290, 419
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 4 cut(s) 32, 89, 98, 368
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 3 cut(s) 160, 179, 184
LpnPI CCDG 5 cut(s) 3, 120, 187, 251, 329
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 4 cut(s) 25, 139, 299, 406
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 1 cut(s) 13
MalI GATC 3 cut(s) 162, 181, 186
MboI GATC 3 cut(s) 160, 179, 184
MboII GAAGA 5 cut(s) 44, 122, 155, 189, 254
MflI RGATCY 2 cut(s) 160, 179
MluCI AATT 2 cut(s) 42, 216
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 322
MnlI CCTC 5 cut(s) 41, 229, 264, 360, 381
MslI CAYNNNNRTG 1 cut(s) 33
MspI CCGG 1 cut(s) 174
NcoI CCATGG 2 cut(s) 85, 94
NdeII GATC 3 cut(s) 160, 179, 184
NlaIII CATG 4 cut(s) 32, 89, 98, 368
NmuCI GTSAC 1 cut(s) 13
PkrI GCNGC 2 cut(s) 7, 40
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PspPI GGNCC 2 cut(s) 248, 368
PsuI RGATCY 2 cut(s) 160, 179
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
RseI CAYNNNNRTG 1 cut(s) 33
SatI GCNGC 2 cut(s) 6, 39
Sau3AI GATC 3 cut(s) 160, 179, 184
Sau96I GGNCC 2 cut(s) 248, 368
SchI GAGTC 1 cut(s) 278
SetI ASST 5 cut(s) 63, 209, 240, 256, 357
SfaNI GCATC 4 cut(s) 25, 139, 299, 406
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 33
Sse9I AATT 2 cut(s) 42, 216
StyI CCWWGG 2 cut(s) 85, 94
TaiI ACGT 1 cut(s) 357
TasI AATT 2 cut(s) 42, 216
TatI WGTACW 1 cut(s) 64
TseFI GTSAC 1 cut(s) 13
TseI GCWGC 2 cut(s) 5, 38
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 2 cut(s) 45, 353
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 1 cut(s) 216
XcmI CCANNNNNNNNNTGG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.