MD14G1103600.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013364
Physical Location & Seq
Reverse (-)
15994854 .. 15997513
2660 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1103600.v1.1.491

Sequence Viewer

Length: 513 bp
ATGAGGTACGTGAAGCCGCTGAACTTGTTCCAGGATCTGCTCAAGGCCAATACTCTGGAACGCGGGCGGTTGCTGGGTTTGGACGTCGGTGACAAGTACGTCGGCGTAGCCGTTTCGGATCTTCATAACAAGATTGCGTCGCCCTTGAGTGTTCTGTTGAGGAAGAAATCAAATATTGGTTTGATGGCCGCCGATTTTCAGAGTTTGATTTCTGAGCTTTCTCTGGTGGGCTTCATTGTTGGATACCCGTTCGACAGACAAAAAGGGACTCCTGATGCTGTCCAAGTGAAGCTTTTCATTGACGACCTCTCTAAGACAAGAAAACTGGAAGGCTTAAAGTATACATATTGGGACGAGGGATTTACATCAAAGAATGTGGAACTACTGTTGAAGCCTTTGAATCTGCCTCCAGTTCAGTCCAAGACAATTATGGACAAGTTTGCTGCTGTTGGTATACTCCAGGGATACTTGGATTATGTGAACAGAGAGCTGAAGTCTGACGTCACTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RuvX

Protein Analysis

171

Amino Acids

19.06

Weight (kDa)

9.18

Isoelectric Point (pI)

19.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuvX PF03652 23 - 158 4e-27 Holliday junction resolvase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52905
fragaria_vesca FvH4_5g02300 FvH4_5g02300 FvH4_5g02300
malus_domestica MD14G1103600.v1.1
prunus_persica Prupe.5G098900_v2.0.a1
pyrus_communis pycom14g09950
rosa_chinensis RchiOBHm_Chr7g0197851
rosa_laevigata RLG00000003905
rosa_roxburghii Rroxscaffold_3G00258140
rosa_rugosa Rorug07G0044000
rosa_samantha Rh7AG167900 Rh7AG168000 Rh7BG170800 Rh7CG177200 Rh7CG177300 Rh7DG169700
rosa_wichuraiana Rw7G014630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
AatII GACGTC 2 cut(s) 87, 504
AccI GTMKAC 2 cut(s) 341, 454
AccII CGCG 1 cut(s) 63
AciI CCGC 4 cut(s) 17, 63, 67, 189
AclWI GGATC 2 cut(s) 42, 126
AcoI YGGCCR 1 cut(s) 186
AcuI CTGAAG 1 cut(s) 512
AcyI GRCGYC 2 cut(s) 84, 501
AfaI GTAC 2 cut(s) 8, 98
AgsI TTSAA 2 cut(s) 391, 400
AjnI CCWGG 2 cut(s) 30, 459
AluBI AGCT 3 cut(s) 217, 292, 490
AluI AGCT 3 cut(s) 217, 292, 490
AlwI GGATC 2 cut(s) 42, 126
AlwNI CAGNNNCTG 1 cut(s) 37
AoxI GGCC 2 cut(s) 45, 186
ApeKI GCWGC 1 cut(s) 443
Asp700I GAANNNNTTC 2 cut(s) 26, 293
AsuHPI GGTGA 1 cut(s) 101
BbvI GCAGC 1 cut(s) 430
BccI CCATC 1 cut(s) 178
BceAI ACGGC 1 cut(s) 95
BciT130I CCWGG 2 cut(s) 32, 461
BciVI GTATCC 2 cut(s) 236, 458
BfuI GTATCC 2 cut(s) 236, 458
BisI GCNGC 3 cut(s) 17, 189, 444
BlsI GCNGC 3 cut(s) 18, 190, 445
Bme1390I CCNGG 2 cut(s) 32, 461
BmrFI CCNGG 2 cut(s) 32, 461
BmsI GCATC 1 cut(s) 265
BpmI CTGGAG 2 cut(s) 393, 443
BpuEI CTTGAG 2 cut(s) 26, 166
BsaAI YACGTR 1 cut(s) 10
BsaBI GATNNNNATC 1 cut(s) 364
BsaHI GRCGYC 2 cut(s) 84, 501
BsaJI CCNNGG 1 cut(s) 460
Bse1I ACTGG 2 cut(s) 330, 410
Bse8I GATNNNNATC 1 cut(s) 364
BseBI CCWGG 2 cut(s) 32, 461
BseDI CCNNGG 1 cut(s) 460
BseJI GATNNNNATC 1 cut(s) 364
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 2 cut(s) 330, 410
BseXI GCAGC 1 cut(s) 430
BseYI CCCAGC 1 cut(s) 73
Bsh1236I CGCG 1 cut(s) 63
BshFI GGCC 2 cut(s) 47, 188
BslFI GGGAC 2 cut(s) 280, 365
BsmFI GGGAC 2 cut(s) 280, 365
BsnI GGCC 2 cut(s) 47, 188
Bsp143I GATC 2 cut(s) 34, 118
BspACI CCGC 4 cut(s) 17, 63, 67, 189
BspANI GGCC 2 cut(s) 47, 188
BspCNI CTCAG 1 cut(s) 205
BspFNI CGCG 1 cut(s) 63
BspPI GGATC 2 cut(s) 42, 126
BsrI ACTGG 2 cut(s) 330, 410
BssECI CCNNGG 1 cut(s) 460
BssMI GATC 2 cut(s) 34, 118
BssNAI GTATAC 2 cut(s) 342, 455
BssNI GRCGYC 2 cut(s) 84, 501
Bst1107I GTATAC 2 cut(s) 342, 455
Bst2UI CCWGG 2 cut(s) 32, 461
Bst4CI ACNGT 1 cut(s) 387
BstACI GRCGYC 2 cut(s) 84, 501
BstBAI YACGTR 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 65
BstDEI CTNAG 3 cut(s) 213, 312, 507
BstFNI CGCG 1 cut(s) 63
BstKTI GATC 2 cut(s) 37, 121
BstMBI GATC 2 cut(s) 34, 118
BstNI CCWGG 2 cut(s) 32, 461
BstSCI CCNGG 2 cut(s) 30, 459
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 1 cut(s) 430
BstX2I RGATCY 2 cut(s) 34, 118
BstXI CCANNNNNNTGG 1 cut(s) 55
BstYI RGATCY 2 cut(s) 34, 118
BstZ17I GTATAC 2 cut(s) 342, 455
BsuI GTATCC 2 cut(s) 236, 458
BsuRI GGCC 2 cut(s) 47, 188
Cac8I GCNNGC 1 cut(s) 65
CaiI CAGNNNCTG 1 cut(s) 37
CseI GACGC 1 cut(s) 126
Csp6I GTAC 2 cut(s) 7, 97
CspCI CAANNNNNGTGG 2 cut(s) 357, 392
CviQI GTAC 2 cut(s) 7, 97
DdeI CTNAG 3 cut(s) 213, 312, 507
DpnI GATC 2 cut(s) 36, 120
DpnII GATC 2 cut(s) 34, 118
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
EaeI YGGCCR 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 512
EcoRII CCWGG 2 cut(s) 30, 459
FaiI YATR 6 cut(s) 126, 342, 346, 431, 455, 477
FalI AAGNNNNNCTT 2 cut(s) 276, 308
FaqI GGGAC 2 cut(s) 280, 365
FauI CCCGC 1 cut(s) 56
FblI GTMKAC 2 cut(s) 341, 454
Fnu4HI GCNGC 3 cut(s) 17, 189, 444
Fsp4HI GCNGC 3 cut(s) 17, 189, 444
GluI GCNGC 3 cut(s) 17, 189, 444
GsaI CCCAGC 1 cut(s) 77
GsuI CTGGAG 2 cut(s) 393, 443
HaeIII GGCC 2 cut(s) 47, 188
HgaI GACGC 1 cut(s) 126
Hin1I GRCGYC 2 cut(s) 84, 501
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 2 cut(s) 268, 400
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 3 cut(s) 342, 455, 481
Hpy188I TCNGA 4 cut(s) 118, 201, 214, 499
Hpy188III TCNNGA 2 cut(s) 56, 272
Hpy8I GTNNAC 3 cut(s) 342, 455, 481
Hpy99I CGWCG 3 cut(s) 89, 104, 142
HpyAV CCTTC 1 cut(s) 323
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4IV ACGT 4 cut(s) 9, 84, 99, 501
HpyF3I CTNAG 3 cut(s) 213, 312, 507
HpySE526I ACGT 4 cut(s) 9, 84, 99, 501
Hsp92I GRCGYC 2 cut(s) 84, 501
Kzo9I GATC 2 cut(s) 34, 118
Lsp1109I GCAGC 1 cut(s) 430
LweI GCATC 1 cut(s) 265
MaeII ACGT 4 cut(s) 9, 84, 99, 501
MaeIII GTNAC 2 cut(s) 89, 502
MalI GATC 2 cut(s) 36, 120
MboI GATC 2 cut(s) 34, 118
MboII GAAGA 2 cut(s) 113, 175
MflI RGATCY 2 cut(s) 34, 118
MluCI AATT 1 cut(s) 426
MlyI GAGTC 1 cut(s) 262
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 4 cut(s) 153, 317, 349, 417
MroXI GAANNNNTTC 2 cut(s) 26, 293
MseI TTAA 1 cut(s) 335
MspA1I CMGCKG 1 cut(s) 19
MspR9I CCNGG 2 cut(s) 32, 461
MvaI CCWGG 2 cut(s) 32, 461
MvnI CGCG 1 cut(s) 63
NdeII GATC 2 cut(s) 34, 118
NmuCI GTSAC 2 cut(s) 89, 502
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdmI GAANNNNTTC 2 cut(s) 26, 293
PfeI GAWTC 1 cut(s) 400
PfoI TCCNGGA 1 cut(s) 30
PkrI GCNGC 3 cut(s) 18, 190, 445
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Ppu21I YACGTR 1 cut(s) 10
Psp6I CCWGG 2 cut(s) 30, 459
PspFI CCCAGC 1 cut(s) 73
PspGI CCWGG 2 cut(s) 30, 459
PstNI CAGNNNCTG 1 cut(s) 37
PsuI RGATCY 2 cut(s) 34, 118
RsaI GTAC 2 cut(s) 8, 98
RsaNI GTAC 2 cut(s) 7, 97
SaqAI TTAA 1 cut(s) 335
SatI GCNGC 3 cut(s) 17, 189, 444
Sau3AI GATC 2 cut(s) 34, 118
SchI GAGTC 1 cut(s) 262
ScrFI CCNGG 2 cut(s) 32, 461
SetI ASST 9 cut(s) 8, 12, 87, 102, 219, 294, 309, 492, 504
SfaNI GCATC 1 cut(s) 265
SmlI CTYRAG 2 cut(s) 41, 145
SmoI CTYRAG 2 cut(s) 41, 145
Sse9I AATT 1 cut(s) 426
SsiI CCGC 4 cut(s) 17, 63, 67, 189
SspI AATATT 1 cut(s) 175
StyD4I CCNGG 2 cut(s) 30, 459
TaaI ACNGT 1 cut(s) 387
TaiI ACGT 4 cut(s) 12, 87, 102, 504
TaqI TCGA 1 cut(s) 252
TasI AATT 1 cut(s) 426
TauI GCSGC 2 cut(s) 19, 191
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TseFI GTSAC 2 cut(s) 89, 502
TseI GCWGC 1 cut(s) 443
Tsp45I GTSAC 2 cut(s) 89, 502
TspDTI ATGAA 3 cut(s) 113, 223, 286
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XmiI GTMKAC 2 cut(s) 341, 454
XmnI GAANNNNTTC 2 cut(s) 26, 293
ZraI GACGTC 2 cut(s) 85, 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.