Rroxscaffold_3G00258140

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013364
Physical Location & Seq
Reverse (-)
52460285 .. 52462208
1924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00258140.1

Sequence Viewer

Length: 537 bp
ATGAAGTACGTGAGGCCCTTGAACATGTTCCTGGATTTGCACAAGGCAAAAGCTCTCCAACGCAGCGGCCGCTTGCTCGGTTTGGACGTCGGCGACAAGTACGTCGGCCTCGCCGTTTCCGACCTCGATAACAAAATCGCCTCCCCTTTAAGTGTTCTGCTTAGGAAGAAATCAACCCTGGAATTGATGGCCTCTGATTTCCAGAGCTTGGTGTCTAAGCTTTCTCTGGTGGGGTTCGTTGTTGGCTACCCTTTCGACAGACAGCGAGGCACTCCTGAAGCTCTCCAAGTGAAGCTTCTGATTGAGGATCTCTGTAAGACGAGAAAACTTGAAGGCATGAAGTATACGTATTGGGACGAGTGCTTTACTTCAAAGAATGTTGAATTACTGCTGAGGCCATTGAGTTTCCATCCGGTTCAGTCGAAGACAATTGTTGACAAGTTTGCTGCTGTTGGTATACTCCAGGGGTACCTGGATTATGTGAACAGGAAGCTGACATTTGATCAAAAGCCATCACCTTTGCTGAACTCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RuvX

Protein Analysis

178

Amino Acids

20.11

Weight (kDa)

9.47

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuvX PF03652 24 - 159 1.1e-25 Holliday junction resolvase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52905
fragaria_vesca FvH4_5g02300 FvH4_5g02300 FvH4_5g02300
malus_domestica MD14G1103600.v1.1
prunus_persica Prupe.5G098900_v2.0.a1
pyrus_communis pycom14g09950
rosa_chinensis RchiOBHm_Chr7g0197851
rosa_laevigata RLG00000003905
rosa_roxburghii Rroxscaffold_3G00258140
rosa_rugosa Rorug07G0044000
rosa_samantha Rh7AG167900 Rh7AG168000 Rh7BG170800 Rh7CG177200 Rh7CG177300 Rh7DG169700
rosa_wichuraiana Rw7G014630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 101
AatII GACGTC 1 cut(s) 90
Acc65I GGTACC 1 cut(s) 468
AccB1I GGYRCC 1 cut(s) 468
AccB7I CCANNNNNTGG 1 cut(s) 208
AccI GTMKAC 2 cut(s) 344, 457
AciI CCGC 2 cut(s) 66, 70
AclWI GGATC 1 cut(s) 315
AcoI YGGCCR 1 cut(s) 67
AcuI CTGAAG 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 3 cut(s) 8, 101, 470
AfiI CCNNNNNNNGG 1 cut(s) 208
AflIII ACRYGT 1 cut(s) 24
AgsI TTSAA 4 cut(s) 22, 332, 372, 383
AjnI CCWGG 4 cut(s) 30, 177, 462, 471
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AluBI AGCT 6 cut(s) 53, 207, 220, 281, 295, 493
AluI AGCT 6 cut(s) 53, 207, 220, 281, 295, 493
AlwI GGATC 1 cut(s) 315
AoxI GGCC 5 cut(s) 14, 67, 106, 189, 395
ApeKI GCWGC 2 cut(s) 63, 446
Asp700I GAANNNNTTC 1 cut(s) 26
Asp718I GGTACC 1 cut(s) 468
AspS9I GGNCC 1 cut(s) 15
AsuHPI GGTGA 1 cut(s) 507
BanI GGYRCC 1 cut(s) 468
BbsI GAAGAC 1 cut(s) 431
BbvCI CCTCAGC 1 cut(s) 392
BbvI GCAGC 2 cut(s) 75, 433
BccI CCATC 3 cut(s) 181, 417, 520
BceAI ACGGC 1 cut(s) 98
BciT130I CCWGG 4 cut(s) 32, 179, 464, 473
BclI TGATCA 1 cut(s) 502
BisI GCNGC 4 cut(s) 64, 67, 70, 447
BlsI GCNGC 4 cut(s) 65, 68, 71, 448
Bme1390I CCNGG 4 cut(s) 32, 179, 464, 473
BmgT120I GGNCC 1 cut(s) 15
BmiI GGNNCC 1 cut(s) 470
BmrFI CCNGG 4 cut(s) 32, 179, 464, 473
BpiI GAAGAC 1 cut(s) 431
BpmI CTGGAG 1 cut(s) 446
Bpu10I CCTNAGC 2 cut(s) 161, 392
BsaAI YACGTR 2 cut(s) 10, 348
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 2 cut(s) 177, 463
BsaWI WCCGGW 1 cut(s) 412
Bsc4I CCNNNNNNNGG 1 cut(s) 208
BseBI CCWGG 4 cut(s) 32, 179, 464, 473
BseDI CCNNGG 2 cut(s) 177, 463
BseGI GGATG 1 cut(s) 409
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 1 cut(s) 383
BseRI GAGGAG 1 cut(s) 520
BseX3I CGGCCG 1 cut(s) 67
BseXI GCAGC 2 cut(s) 75, 433
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 5 cut(s) 16, 69, 108, 191, 397
BshNI GGYRCC 1 cut(s) 468
BsiEI CGRYCG 1 cut(s) 70
BsiSI CCGG 1 cut(s) 413
BslFI GGGAC 1 cut(s) 368
BslI CCNNNNNNNGG 1 cut(s) 208
BsmFI GGGAC 1 cut(s) 368
BsnI GGCC 5 cut(s) 16, 69, 108, 191, 397
Bsp143I GATC 2 cut(s) 307, 502
BspACI CCGC 2 cut(s) 66, 70
BspANI GGCC 5 cut(s) 16, 69, 108, 191, 397
BspCNI CTCAG 1 cut(s) 384
BspHI TCATGA 1 cut(s) 533
BspLI GGNNCC 1 cut(s) 470
BspPI GGATC 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 468
BssECI CCNNGG 2 cut(s) 177, 463
BssMI GATC 2 cut(s) 307, 502
BssNAI GTATAC 2 cut(s) 345, 458
BssNI GRCGYC 1 cut(s) 87
Bst1107I GTATAC 2 cut(s) 345, 458
Bst2UI CCWGG 4 cut(s) 32, 179, 464, 473
BstACI GRCGYC 1 cut(s) 87
BstBAI YACGTR 2 cut(s) 10, 348
BstC8I GCNNGC 1 cut(s) 74
BstDEI CTNAG 3 cut(s) 161, 216, 392
BstF5I GGATG 1 cut(s) 409
BstKTI GATC 2 cut(s) 310, 505
BstMBI GATC 2 cut(s) 307, 502
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstNI CCWGG 4 cut(s) 32, 179, 464, 473
BstNSI RCATGY 1 cut(s) 28
BstSCI CCNGG 4 cut(s) 30, 177, 462, 471
BstSNI TACGTA 1 cut(s) 348
BstV1I GCAGC 2 cut(s) 75, 433
BstV2I GAAGAC 1 cut(s) 431
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BstZ17I GTATAC 2 cut(s) 345, 458
BstZI CGGCCG 1 cut(s) 67
BsuRI GGCC 5 cut(s) 16, 69, 108, 191, 397
BtsCI GGATG 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 74
CciI TCATGA 1 cut(s) 533
CciNI GCGGCCGC 1 cut(s) 67
Cfr13I GGNCC 1 cut(s) 15
Csp6I GTAC 3 cut(s) 7, 100, 469
CviAII CATG 3 cut(s) 25, 337, 534
CviQI GTAC 3 cut(s) 7, 100, 469
DdeI CTNAG 3 cut(s) 161, 216, 392
DpnI GATC 2 cut(s) 309, 504
DpnII GATC 2 cut(s) 307, 502
DrdI GACNNNNNNGTC 1 cut(s) 101
DseDI GACNNNNNNGTC 1 cut(s) 101
EaeI YGGCCR 1 cut(s) 67
EagI CGGCCG 1 cut(s) 67
EclXI CGGCCG 1 cut(s) 67
Eco105I TACGTA 1 cut(s) 348
Eco52I CGGCCG 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 297
EcoO109I RGGNCCY 1 cut(s) 15
EcoRII CCWGG 4 cut(s) 30, 177, 462, 471
FaeI CATG 3 cut(s) 28, 340, 537
FaiI YATR 6 cut(s) 26, 338, 345, 458, 480, 535
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FaqI GGGAC 1 cut(s) 368
FatI CATG 3 cut(s) 24, 336, 533
FbaI TGATCA 1 cut(s) 502
FblI GTMKAC 2 cut(s) 344, 457
Fnu4HI GCNGC 4 cut(s) 64, 67, 70, 447
FokI GGATG 1 cut(s) 396
Fsp4HI GCNGC 4 cut(s) 64, 67, 70, 447
GluI GCNGC 4 cut(s) 64, 67, 70, 447
GsuI CTGGAG 1 cut(s) 446
HaeIII GGCC 5 cut(s) 16, 69, 108, 191, 397
HapII CCGG 1 cut(s) 413
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 3 cut(s) 28, 340, 537
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HindIII AAGCTT 2 cut(s) 218, 293
HpaII CCGG 1 cut(s) 413
HphI GGTGA 1 cut(s) 507
Hpy166II GTNNAC 4 cut(s) 345, 436, 458, 484
Hpy188I TCNGA 3 cut(s) 121, 196, 300
Hpy188III TCNNGA 3 cut(s) 202, 275, 534
Hpy8I GTNNAC 4 cut(s) 345, 436, 458, 484
Hpy99I CGWCG 2 cut(s) 92, 107
HpyAV CCTTC 1 cut(s) 326
HpyCH4IV ACGT 4 cut(s) 9, 87, 102, 347
HpyCH4V TGCA 1 cut(s) 40
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 3 cut(s) 161, 216, 392
HpySE526I ACGT 4 cut(s) 9, 87, 102, 347
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 3 cut(s) 28, 340, 537
KpnI GGTACC 1 cut(s) 472
Ksp22I TGATCA 1 cut(s) 502
Kzo9I GATC 2 cut(s) 307, 502
Lsp1109I GCAGC 2 cut(s) 75, 433
MaeII ACGT 4 cut(s) 9, 87, 102, 347
MalI GATC 2 cut(s) 309, 504
MboI GATC 2 cut(s) 307, 502
MboII GAAGA 2 cut(s) 178, 436
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 1 cut(s) 307
MluCI AATT 3 cut(s) 182, 383, 429
MmeI TCCRAC 2 cut(s) 82, 144
MnlI CCTC 8 cut(s) 6, 119, 134, 151, 202, 260, 298, 387
MroXI GAANNNNTTC 1 cut(s) 26
MseI TTAA 1 cut(s) 149
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 1 cut(s) 413
MspR9I CCNGG 4 cut(s) 32, 179, 464, 473
MunI CAATTG 1 cut(s) 429
MvaI CCWGG 4 cut(s) 32, 179, 464, 473
MwoI GCNNNNNNNGC 1 cut(s) 69
NdeII GATC 2 cut(s) 307, 502
NlaIII CATG 3 cut(s) 28, 340, 537
NlaIV GGNNCC 1 cut(s) 470
NotI GCGGCCGC 1 cut(s) 67
NspI RCATGY 1 cut(s) 28
PagI TCATGA 1 cut(s) 533
PciI ACATGT 1 cut(s) 24
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 111, 117
PdmI GAANNNNTTC 1 cut(s) 26
PflMI CCANNNNNTGG 1 cut(s) 208
PfoI TCCNGGA 1 cut(s) 30
PkrI GCNGC 4 cut(s) 65, 68, 71, 448
Ppu21I YACGTR 2 cut(s) 10, 348
PscI ACATGT 1 cut(s) 24
Psp6I CCWGG 4 cut(s) 30, 177, 462, 471
PspGI CCWGG 4 cut(s) 30, 177, 462, 471
PspN4I GGNNCC 1 cut(s) 470
PspPI GGNCC 1 cut(s) 15
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 3 cut(s) 8, 101, 470
RsaNI GTAC 3 cut(s) 7, 100, 469
SaqAI TTAA 1 cut(s) 149
SatI GCNGC 4 cut(s) 64, 67, 70, 447
Sau3AI GATC 2 cut(s) 307, 502
Sau96I GGNCC 1 cut(s) 15
ScrFI CCNGG 4 cut(s) 32, 179, 464, 473
SnaBI TACGTA 1 cut(s) 348
Sse9I AATT 3 cut(s) 182, 383, 429
SsiI CCGC 2 cut(s) 66, 70
StyD4I CCNGG 4 cut(s) 30, 177, 462, 471
TaiI ACGT 4 cut(s) 12, 90, 105, 350
TaqI TCGA 3 cut(s) 126, 255, 422
TasI AATT 3 cut(s) 182, 383, 429
TauI GCSGC 2 cut(s) 69, 72
Tru1I TTAA 1 cut(s) 149
Tru9I TTAA 1 cut(s) 149
TseI GCWGC 2 cut(s) 63, 446
TspDTI ATGAA 2 cut(s) 17, 353
Van91I CCANNNNNTGG 1 cut(s) 208
XceI RCATGY 1 cut(s) 28
XmiI GTMKAC 2 cut(s) 344, 457
XmnI GAANNNNTTC 1 cut(s) 26
ZraI GACGTC 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.