MD14G1151600.v1.1

May serve as docking site to facilitate the association of other proteins to the plasma membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009060
Physical Location & Seq
Forward (+)
24498966 .. 24500980
2015 bp
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UTR
Exon/CDS
Intron
MD14G1151600.v1.1.491

Sequence Viewer

Length: 357 bp
ATGCCGGAGGAGGATTTGGTGGACATAAAGTTCCGGCTGTACGACGGCACCGATATCGGGCCGTTCAGCTACTCATCAGCCTCCACCGTCGATATGCTCAAGCAGAGGGTCGTCTCCGATTGGCCCAGAGGCAAGACTATGACGCCGAAGACGGCGAATGAAGTGAAACTGATGAGTTTTGGCAAGATTTTGGAGAACAACAAGACAGTGGGTCAGTGTAAATTACCTTTGGCTGATATTGGTGGGGGAATTATCATAATGCTTGTTGTTGTACAGCAAGCTATCGAAAAAGCTAAAACAGAAAAGAAGATTGATGATCGGCCCCGGAAAGTTGTCTGTTCATGTTCCATATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rad60-SLD_2

Protein Analysis

119

Amino Acids

13.09

Weight (kDa)

8.79

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD_2 PF13881 5 - 116 1.1e-37 Ubiquitin-2 like Rad60 SUMO-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 47
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 2 cut(s) 41, 273
AfiI CCNNNNNNNGG 1 cut(s) 57
AhdI GACNNNNNGTC 1 cut(s) 210
AloI GAACNNNNNNTCC 2 cut(s) 14, 46
AluBI AGCT 3 cut(s) 69, 281, 293
AluI AGCT 3 cut(s) 69, 281, 293
Alw26I GTCTC 1 cut(s) 118
AoxI GGCC 3 cut(s) 59, 122, 320
AspS9I GGNCC 3 cut(s) 59, 123, 321
AsuC2I CCSGG 1 cut(s) 325
BanI GGYRCC 1 cut(s) 47
BbsI GAAGAC 1 cut(s) 155
BceAI ACGGC 3 cut(s) 46, 61, 168
BcgI CGANNNNNNTGC 2 cut(s) 37, 71
BcnI CCSGG 1 cut(s) 325
BcoDI GTCTC 1 cut(s) 118
Bme1390I CCNGG 1 cut(s) 325
BmeRI GACNNNNNGTC 1 cut(s) 210
BmgT120I GGNCC 3 cut(s) 59, 123, 321
BmiI GGNNCC 2 cut(s) 49, 323
BmrFI CCNGG 1 cut(s) 325
BpiI GAAGAC 1 cut(s) 155
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 325
BsaHI GRCGYC 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 323
Bsc4I CCNNNNNNNGG 1 cut(s) 57
BseDI CCNNGG 1 cut(s) 323
BseLI CCNNNNNNNGG 1 cut(s) 57
BseRI GAGGAG 1 cut(s) 23
BshFI GGCC 3 cut(s) 61, 124, 322
BshNI GGYRCC 1 cut(s) 47
BsiSI CCGG 3 cut(s) 5, 34, 325
BslI CCNNNNNNNGG 1 cut(s) 57
BsmAI GTCTC 1 cut(s) 118
BsmBI CGTCTC 1 cut(s) 118
BsnI GGCC 3 cut(s) 61, 124, 322
Bsp1407I TGTACA 1 cut(s) 271
Bsp143I GATC 1 cut(s) 316
BspANI GGCC 3 cut(s) 61, 124, 322
BspLI GGNNCC 2 cut(s) 49, 323
BspT107I GGYRCC 1 cut(s) 47
BsrGI TGTACA 1 cut(s) 271
BssECI CCNNGG 1 cut(s) 323
BssMI GATC 1 cut(s) 316
BssNI GRCGYC 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 88, 208
BstACI GRCGYC 1 cut(s) 143
BstAUI TGTACA 1 cut(s) 271
BstC8I GCNNGC 1 cut(s) 279
BstKTI GATC 1 cut(s) 319
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 1 cut(s) 316
BstSCI CCNGG 1 cut(s) 323
BstV2I GAAGAC 1 cut(s) 155
BsuRI GGCC 3 cut(s) 61, 124, 322
BtsIMutI CAGTG 2 cut(s) 213, 221
Cac8I GCNNGC 1 cut(s) 279
Cfr13I GGNCC 3 cut(s) 59, 123, 321
CseI GACGC 1 cut(s) 151
Csp6I GTAC 2 cut(s) 40, 272
CviAII CATG 1 cut(s) 342
CviJI RGCY 9 cut(s) 37, 61, 69, 80, 124, 233, 281, 293, 322
CviKI_1 RGCY 9 cut(s) 37, 61, 69, 80, 124, 233, 281, 293, 322
CviQI GTAC 2 cut(s) 40, 272
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
DriI GACNNNNNGTC 1 cut(s) 210
Eam1105I GACNNNNNGTC 1 cut(s) 210
Eco32I GATATC 1 cut(s) 55
EcoRV GATATC 1 cut(s) 55
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 1 cut(s) 345
FaiI YATR 6 cut(s) 26, 95, 140, 257, 343, 350
FatI CATG 1 cut(s) 341
HaeIII GGCC 3 cut(s) 61, 124, 322
HapII CCGG 3 cut(s) 5, 34, 325
HgaI GACGC 1 cut(s) 151
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 1 cut(s) 345
HpaII CCGG 3 cut(s) 5, 34, 325
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 1 cut(s) 118
Hpy8I GTNNAC 1 cut(s) 22
Hpy99I CGWCG 2 cut(s) 47, 92
HpyCH4III ACNGT 2 cut(s) 88, 208
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 1 cut(s) 345
Kzo9I GATC 1 cut(s) 316
LpnPI CCDG 4 cut(s) 18, 47, 139, 338
MalI GATC 1 cut(s) 318
MboI GATC 1 cut(s) 316
MboII GAAGA 2 cut(s) 160, 319
MluCI AATT 2 cut(s) 221, 249
MnlI CCTC 4 cut(s) 4, 91, 99, 122
MspI CCGG 3 cut(s) 5, 34, 325
MspR9I CCNGG 1 cut(s) 325
NciI CCSGG 1 cut(s) 325
NdeII GATC 1 cut(s) 316
NlaIII CATG 1 cut(s) 345
NlaIV GGNNCC 2 cut(s) 49, 323
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PspN4I GGNNCC 2 cut(s) 49, 323
PspPI GGNCC 3 cut(s) 59, 123, 321
RsaI GTAC 2 cut(s) 41, 273
RsaNI GTAC 2 cut(s) 40, 272
Sau3AI GATC 1 cut(s) 316
Sau96I GGNCC 3 cut(s) 59, 123, 321
ScrFI CCNGG 1 cut(s) 325
SetI ASST 4 cut(s) 71, 229, 283, 295
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 2 cut(s) 221, 249
StyD4I CCNGG 1 cut(s) 323
TaaI ACNGT 2 cut(s) 88, 208
TaqI TCGA 2 cut(s) 90, 285
TasI AATT 2 cut(s) 221, 249
TatI WGTACW 1 cut(s) 271
TscAI CASTG 2 cut(s) 213, 221
TspDTI ATGAA 2 cut(s) 174, 330
TspRI CASTG 2 cut(s) 213, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.