MD14G1196400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011434
Physical Location & Seq
Reverse (-)
28671160 .. 28673879
2720 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1196400.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGAGGAGCCCCAATCTGCTCAATCTGCCCAGTCTGGAAAGCTTCTTCGGTATGCTCTCCGTTCGGGGACTAAATCGAAGGAGGAGAAGCCGCCGGTGGCGGCCGCTGAACCCTCCCCTAACAATTCTGCCGCCAAGAGGGAAAGGCCTGCATCAAATATGAGTAAAAGTGTGGGGGTTCTTGATCTCTCTGGCAAAGACAAGCCTGCCAAGCCGACCCGAAGGTTTTCAATTCCTTCCAAGCCGCCCACTACTCCGGCTCCGAAACTTGGTGGCAACATCACTCAGATTTCCGAGGCTAGATCAAGGAGGTCTGTGAGAAGTGACTCCCCTGCTTCTTCTGATGTTTCTAGACCAACAAGCCGGAAGAAGTTTAATGTTCTGGCCTCACCATCTTACTGGCTGCAGCAGATTAAGCTGTCTGAGGCTGCTGGCAAGCACTTGATTTCACTCGGGTTTTTCAAACTAGCCTTGGAGGCTGAATGTGAGTCTCTTCAACTGCGTTATGAGCTCAAATCTTATGTTGATCGCTACGAGCTTAGTGACCTTGAAGACCCTCTGAAGGAATTACTGGAGAGCAAAAGTGTTGCAGTAAATGAGCAGGCTCAGGTCTCCGAAACCTGTTCTCAGGTGCTCGAAGAGGGGACTCGATCTTCTGATGATGATGCCAAGAGCTGCTCTTCTACAATGGCAAGTGGGAAACTGAAACCCAAGTCATTGAACATTAAAGCTGCTCAAGTTTCTCCAGAGAAGGCCACAGCCAAGAAGGAGATTTCCTCGAAAAGCACTCCAGCAGCCAGGACCAGGTCATCTTCGGTGAAGAGTACTTCGAATTCAGGACCTGTATCTGACAATGGAGCTCGCGTGTCTACTGTGAAGAAACCCCAGAAGCCAAACGTGCAAGATTCTAACAAAGTAAAGGACAGGAAGAAGAAGCCGGGGAATCAAACTGCTTGTGAACAAGACTCAATTAACCCTGCCGCAGAAGGACTTCAGGAGAACAAAGAAAACCAGGATGCCCCCTCGACGGAAGCTGCGACTGAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

350

Amino Acids

37.56

Weight (kDa)

9.48

Isoelectric Point (pI)

64.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62550
fragaria_vesca FvH4_5g15300 FvH4_5g15300 FvH4_5g15300
malus_domestica MD01G1041800.v1.1 MD06G1189400.v1.1 MD14G1196400.v1.1
prunus_persica Prupe.5G188200_v2.0.a1
pyrus_communis pycom06g16830 pycom14g16310
rosa_chinensis RchiOBHm_Chr7g0180761
rosa_laevigata RLG00000005232
rosa_multiflora Rmu_sc0007457.1_g000012
rosa_roxburghii Rroxscaffold_3G00272560
rosa_rugosa Rorug06G0435800
rosa_samantha Rh7AG037500 Rh7BG037200 Rh7CG038900 Rh7DG037400
rosa_wichuraiana Rw7G003090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 869
AccII CGCG 1 cut(s) 864
AciI CCGC 6 cut(s) 92, 101, 105, 132, 245, 981
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 1 cut(s) 832
AcuI CTGAAG 2 cut(s) 581, 977
AfaI GTAC 1 cut(s) 826
AfiI CCNNNNNNNGG 5 cut(s) 138, 269, 562, 804, 1027
AgsI TTSAA 5 cut(s) 231, 463, 497, 551, 721
AjnI CCWGG 3 cut(s) 797, 803, 1011
AluBI AGCT 8 cut(s) 43, 418, 511, 538, 675, 731, 860, 1034
AluI AGCT 8 cut(s) 43, 418, 511, 538, 675, 731, 860, 1034
Alw21I GWGCWC 3 cut(s) 513, 636, 862
Alw26I GTCTC 2 cut(s) 495, 616
AlwNI CAGNNNCTG 1 cut(s) 842
Ama87I CYCGRG 1 cut(s) 452
AoxI GGCC 4 cut(s) 102, 146, 384, 753
ApeKI GCWGC 7 cut(s) 403, 406, 428, 675, 731, 794, 1034
ApoI RAATTY 1 cut(s) 832
Asp700I GAANNNNTTC 2 cut(s) 226, 990
AspS9I GGNCC 2 cut(s) 801, 839
AsuC2I CCSGG 1 cut(s) 939
AsuHPI GGTGA 2 cut(s) 381, 829
AsuII TTCGAA 1 cut(s) 830
AvaI CYCGRG 1 cut(s) 452
AvaII GGWCC 2 cut(s) 801, 839
BanII GRGCYC 3 cut(s) 12, 513, 862
BbsI GAAGAC 1 cut(s) 558
Bbv12I GWGCWC 3 cut(s) 513, 636, 862
BbvI GCAGC 7 cut(s) 390, 415, 418, 662, 718, 806, 1021
BccI CCATC 1 cut(s) 400
BciT130I CCWGG 3 cut(s) 799, 805, 1013
BcnI CCSGG 1 cut(s) 939
BcoDI GTCTC 2 cut(s) 495, 616
BfaI CTAG 3 cut(s) 300, 351, 467
BfmI CTRYAG 1 cut(s) 404
BglI GCCNNNNNGGC 1 cut(s) 476
BmcAI AGTACT 1 cut(s) 826
Bme1390I CCNGG 4 cut(s) 799, 805, 939, 1013
Bme18I GGWCC 2 cut(s) 801, 839
BmeT110I CYCGRG 1 cut(s) 452
BmgT120I GGNCC 2 cut(s) 801, 839
BmiI GGNNCC 2 cut(s) 9, 261
BmrFI CCNGG 4 cut(s) 799, 805, 939, 1013
BmrI ACTGGG 1 cut(s) 25
BmsI GCATC 3 cut(s) 161, 655, 1006
BmuI ACTGGG 1 cut(s) 25
BpiI GAAGAC 1 cut(s) 558
BplI GAGNNNNNCTC 2 cut(s) 761, 793
BpmI CTGGAG 3 cut(s) 593, 729, 774
Bpu10I CCTNAGC 1 cut(s) 606
Bpu14I TTCGAA 1 cut(s) 830
BpuEI CTTGAG 1 cut(s) 720
BpuMI CCSGG 1 cut(s) 939
BsaI GGTCTC 1 cut(s) 616
BsaJI CCNNGG 3 cut(s) 294, 471, 938
BsaXI ACNNNNNCTCC 4 cut(s) 244, 274, 849, 879
Bsc4I CCNNNNNNNGG 5 cut(s) 138, 269, 562, 804, 1027
Bse118I RCCGGY 1 cut(s) 94
Bse1I ACTGG 3 cut(s) 31, 404, 576
BseBI CCWGG 3 cut(s) 799, 805, 1013
BseDI CCNNGG 3 cut(s) 294, 471, 938
BseGI GGATG 1 cut(s) 1021
BseLI CCNNNNNNNGG 5 cut(s) 138, 269, 562, 804, 1027
BseMII CTCAG 4 cut(s) 299, 414, 620, 641
BseNI ACTGG 3 cut(s) 31, 404, 576
BseRI GAGGAG 2 cut(s) 20, 98
BseX3I CGGCCG 1 cut(s) 102
BseXI GCAGC 7 cut(s) 390, 415, 418, 662, 718, 806, 1021
Bsh1236I CGCG 1 cut(s) 864
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 4 cut(s) 104, 148, 386, 755
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 3 cut(s) 513, 636, 862
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 4 cut(s) 95, 257, 364, 938
BslFI GGGAC 2 cut(s) 82, 658
BslI CCNNNNNNNGG 5 cut(s) 138, 269, 562, 804, 1027
BsmAI GTCTC 2 cut(s) 495, 616
BsmFI GGGAC 2 cut(s) 82, 658
BsnI GGCC 4 cut(s) 104, 148, 386, 755
Bso31I GGTCTC 1 cut(s) 616
BsoBI CYCGRG 1 cut(s) 452
Bsp119I TTCGAA 1 cut(s) 830
Bsp1286I GDGCHC 4 cut(s) 12, 513, 636, 862
Bsp143I GATC 4 cut(s) 184, 302, 526, 650
BspACI CCGC 6 cut(s) 92, 101, 105, 132, 245, 981
BspANI GGCC 4 cut(s) 104, 148, 386, 755
BspCNI CTCAG 4 cut(s) 298, 415, 619, 640
BspFNI CGCG 1 cut(s) 864
BspLI GGNNCC 2 cut(s) 9, 261
BspMAI CTGCAG 1 cut(s) 408
BspQI GCTCTTC 1 cut(s) 685
BspT104I TTCGAA 1 cut(s) 830
BspTNI GGTCTC 1 cut(s) 616
BsrFI RCCGGY 1 cut(s) 94
BsrI ACTGG 3 cut(s) 31, 404, 576
BssAI RCCGGY 1 cut(s) 94
BssECI CCNNGG 3 cut(s) 294, 471, 938
BssMI GATC 4 cut(s) 184, 302, 526, 650
BssT1I CCWWGG 1 cut(s) 471
Bst2UI CCWGG 3 cut(s) 799, 805, 1013
Bst4CI ACNGT 1 cut(s) 874
Bst6I CTCTTC 4 cut(s) 498, 633, 685, 815
BstBI TTCGAA 1 cut(s) 830
BstC8I GCNNGC 6 cut(s) 150, 207, 433, 437, 603, 862
BstDEI CTNAG 5 cut(s) 285, 423, 539, 606, 627
BstF5I GGATG 1 cut(s) 1021
BstFNI CGCG 1 cut(s) 864
BstKTI GATC 4 cut(s) 187, 305, 529, 653
BstMAI GTCTC 2 cut(s) 495, 616
BstMBI GATC 4 cut(s) 184, 302, 526, 650
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 6 cut(s) 26, 211, 415, 476, 508, 898
BstNI CCWGG 3 cut(s) 799, 805, 1013
BstSCI CCNGG 4 cut(s) 797, 803, 937, 1011
BstSFI CTRYAG 1 cut(s) 404
BstUI CGCG 1 cut(s) 864
BstV1I GCAGC 7 cut(s) 390, 415, 418, 662, 718, 806, 1021
BstV2I GAAGAC 1 cut(s) 558
BstXI CCANNNNNNTGG 1 cut(s) 399
BstZI CGGCCG 1 cut(s) 102
BsuRI GGCC 4 cut(s) 104, 148, 386, 755
BtsCI GGATG 1 cut(s) 1021
Cac8I GCNNGC 6 cut(s) 150, 207, 433, 437, 603, 862
CaiI CAGNNNCTG 1 cut(s) 842
CciNI GCGGCCGC 1 cut(s) 102
Cfr10I RCCGGY 1 cut(s) 94
Cfr13I GGNCC 2 cut(s) 801, 839
CsiI ACCWGGT 1 cut(s) 803
Csp6I GTAC 1 cut(s) 825
CviQI GTAC 1 cut(s) 825
DdeI CTNAG 5 cut(s) 285, 423, 539, 606, 627
DpnI GATC 4 cut(s) 186, 304, 528, 652
DpnII GATC 4 cut(s) 184, 302, 526, 650
EaeI YGGCCR 1 cut(s) 102
EagI CGGCCG 1 cut(s) 102
Eam1104I CTCTTC 4 cut(s) 498, 633, 685, 815
EarI CTCTTC 4 cut(s) 498, 633, 685, 815
Ecl136II GAGCTC 2 cut(s) 511, 860
EclXI CGGCCG 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 471
Eco147I AGGCCT 1 cut(s) 148
Eco24I GRGCYC 3 cut(s) 12, 513, 862
Eco31I GGTCTC 1 cut(s) 616
Eco47I GGWCC 2 cut(s) 801, 839
Eco52I CGGCCG 1 cut(s) 102
Eco53kI GAGCTC 2 cut(s) 511, 860
Eco57I CTGAAG 2 cut(s) 581, 977
Eco88I CYCGRG 1 cut(s) 452
EcoICRI GAGCTC 2 cut(s) 511, 860
EcoO109I RGGNCCY 1 cut(s) 839
EcoRI GAATTC 1 cut(s) 832
EcoRII CCWGG 3 cut(s) 797, 803, 1011
EcoT14I CCWWGG 1 cut(s) 471
EcoT38I GRGCYC 3 cut(s) 12, 513, 862
ErhI CCWWGG 1 cut(s) 471
FaiI YATR 4 cut(s) 54, 161, 507, 522
FaqI GGGAC 2 cut(s) 82, 658
FblI GTMKAC 1 cut(s) 869
FokI GGATG 1 cut(s) 1028
FriOI GRGCYC 3 cut(s) 12, 513, 862
FspBI CTAG 3 cut(s) 300, 351, 467
GsuI CTGGAG 3 cut(s) 593, 729, 774
HaeIII GGCC 4 cut(s) 104, 148, 386, 755
HapII CCGG 4 cut(s) 95, 257, 364, 938
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 6 cut(s) 326, 488, 646, 905, 943, 965
HpaII CCGG 4 cut(s) 95, 257, 364, 938
HphI GGTGA 2 cut(s) 381, 829
Hpy166II GTNNAC 2 cut(s) 870, 959
Hpy188I TCNGA 9 cut(s) 264, 288, 295, 343, 424, 561, 616, 658, 850
Hpy188III TCNNGA 6 cut(s) 36, 182, 351, 746, 837, 995
Hpy8I GTNNAC 2 cut(s) 870, 959
Hpy99I CGWCG 1 cut(s) 1030
HpyAV CCTTC 7 cut(s) 73, 216, 246, 556, 745, 760, 980
HpyCH4III ACNGT 1 cut(s) 874
HpyCH4IV ACGT 1 cut(s) 897
HpyCH4V TGCA 4 cut(s) 152, 406, 590, 901
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 211, 415, 476, 508, 898
HpyF3I CTNAG 5 cut(s) 285, 423, 539, 606, 627
HpySE526I ACGT 1 cut(s) 897
Kzo9I GATC 4 cut(s) 184, 302, 526, 650
LguI GCTCTTC 1 cut(s) 685
LmnI GCTCC 3 cut(s) 7, 265, 857
Lsp1109I GCAGC 7 cut(s) 390, 415, 418, 662, 718, 806, 1021
LweI GCATC 3 cut(s) 161, 655, 1006
MabI ACCWGGT 1 cut(s) 803
MaeI CTAG 3 cut(s) 300, 351, 467
MaeII ACGT 1 cut(s) 897
MaeIII GTNAC 2 cut(s) 323, 542
MalI GATC 4 cut(s) 186, 304, 528, 652
MboI GATC 4 cut(s) 184, 302, 526, 650
MhlI GDGCHC 4 cut(s) 12, 513, 636, 862
MluCI AATT 5 cut(s) 124, 231, 566, 832, 969
MlyI GAGTC 4 cut(s) 320, 497, 640, 959
MroXI GAANNNNTTC 2 cut(s) 226, 990
MseI TTAA 4 cut(s) 375, 414, 726, 972
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 4 cut(s) 95, 257, 364, 938
MspR9I CCNGG 4 cut(s) 799, 805, 939, 1013
MvaI CCWGG 3 cut(s) 799, 805, 1013
MvnI CGCG 1 cut(s) 864
MwoI GCNNNNNNNGC 6 cut(s) 26, 211, 415, 476, 508, 898
NciI CCSGG 1 cut(s) 939
NdeII GATC 4 cut(s) 184, 302, 526, 650
NlaIV GGNNCC 2 cut(s) 9, 261
NmuCI GTSAC 2 cut(s) 323, 542
NotI GCGGCCGC 1 cut(s) 102
NspV TTCGAA 1 cut(s) 830
PceI AGGCCT 1 cut(s) 148
PciSI GCTCTTC 1 cut(s) 685
PcsI WCGNNNNNNNCGW 1 cut(s) 1034
PdmI GAANNNNTTC 2 cut(s) 226, 990
PfeI GAWTC 2 cut(s) 905, 943
PflFI GACNNNGTC 1 cut(s) 805
PleI GAGTC 4 cut(s) 320, 496, 640, 959
PpsI GAGTC 4 cut(s) 320, 496, 640, 959
PpuMI RGGWCCY 1 cut(s) 839
Psp124BI GAGCTC 2 cut(s) 513, 862
Psp5II RGGWCCY 1 cut(s) 839
Psp6I CCWGG 3 cut(s) 797, 803, 1011
PspGI CCWGG 3 cut(s) 797, 803, 1011
PspN4I GGNNCC 2 cut(s) 9, 261
PspPI GGNCC 2 cut(s) 801, 839
PspPPI RGGWCCY 1 cut(s) 839
PstI CTGCAG 1 cut(s) 408
PstNI CAGNNNCTG 1 cut(s) 842
PsyI GACNNNGTC 1 cut(s) 805
RsaI GTAC 1 cut(s) 826
RsaNI GTAC 1 cut(s) 825
SacI GAGCTC 2 cut(s) 513, 862
SapI GCTCTTC 1 cut(s) 685
SaqAI TTAA 4 cut(s) 375, 414, 726, 972
Sau3AI GATC 4 cut(s) 184, 302, 526, 650
Sau96I GGNCC 2 cut(s) 801, 839
ScaI AGTACT 1 cut(s) 826
SchI GAGTC 4 cut(s) 320, 497, 640, 959
ScrFI CCNGG 4 cut(s) 799, 805, 939, 1013
SduI GDGCHC 4 cut(s) 12, 513, 636, 862
SexAI ACCWGGT 1 cut(s) 803
SfaNI GCATC 3 cut(s) 161, 655, 1006
SfcI CTRYAG 1 cut(s) 404
SfuI TTCGAA 1 cut(s) 830
SgrAI CRCCGGYG 1 cut(s) 94
SinI GGWCC 2 cut(s) 801, 839
SmlI CTYRAG 1 cut(s) 735
SmoI CTYRAG 1 cut(s) 735
Sse9I AATT 5 cut(s) 124, 231, 566, 832, 969
SseBI AGGCCT 1 cut(s) 148
SsiI CCGC 6 cut(s) 92, 101, 105, 132, 245, 981
SspMI CTAG 3 cut(s) 300, 351, 467
SstI GAGCTC 2 cut(s) 513, 862
StuI AGGCCT 1 cut(s) 148
StyD4I CCNGG 4 cut(s) 797, 803, 937, 1011
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 1 cut(s) 874
TaiI ACGT 1 cut(s) 900
TaqI TCGA 6 cut(s) 77, 636, 649, 779, 830, 1025
TasI AATT 5 cut(s) 124, 231, 566, 832, 969
TatI WGTACW 1 cut(s) 824
TauI GCSGC 6 cut(s) 94, 104, 107, 134, 247, 983
TfiI GAWTC 2 cut(s) 905, 943
Tru1I TTAA 4 cut(s) 375, 414, 726, 972
Tru9I TTAA 4 cut(s) 375, 414, 726, 972
TseFI GTSAC 2 cut(s) 323, 542
TseI GCWGC 7 cut(s) 403, 406, 428, 675, 731, 794, 1034
Tsp45I GTSAC 2 cut(s) 323, 542
TspGWI ACGGA 2 cut(s) 50, 1043
Tth111I GACNNNGTC 1 cut(s) 805
VpaK11BI GGWCC 2 cut(s) 801, 839
XapI RAATTY 1 cut(s) 832
XbaI TCTAGA 1 cut(s) 350
XmiI GTMKAC 1 cut(s) 869
XmnI GAANNNNTTC 2 cut(s) 226, 990
XspI CTAG 3 cut(s) 300, 351, 467
ZrmI AGTACT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.