Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0012277

Physical Location & Seq

Genomic Coordinates Reverse (-)

28902140 .. 28902940

Gene Structure 801 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1199400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 801 bp

ATGGTAGATGCACTCGAGCTGTTTGATTTAATTGCTGACCGAAATGTTTTTTCATGGAATATCATGATTTCCGGTTATAACCACATTTCGTTATTCGAGAAGGCATGGGAAATGTTTTGTCCGATGCATGCTTCGGGTTTTGGGCCCGATGAGTTTGCCTTTGGGAGTGTTCTTTCGGCTTGCATTGCCTTGCAAGCTCCTATTTTTGGGAAGCAGGTCTATTCGCTTGCAATGAAAAATGGGTTCTTTTCGAATGGTTATGTTCAGTCTGGAATGATAGACTTGTTCGCGAAAAATTGCAGCTTTGAAGATGCTTTGAGGGTTTTTCATGATGTTTCATGTCACAATGTTGTGGTTTGGAATGCTATTATCTCTGGGCCTGTGAGAAAGGGAGAGAACAGGGTTGCGTTGCATCTTTTTCGGAAAATGTGTCGTGGATTCCTCTTGCCAAATAGTTTTACATTTTCTAGCGTTTTAACCGCTTGTGCTGCACTTGAAGAAATTGAAGTTGGGAAGGAAGTTCAAGGGTTGGTGATTAAACGCGGTGCAAAAGATGTCTTTGTTGGGACTACTATTGTAGATTTATATGCCAAATGTGGAGAAATGAATGAAGCTGTCAAGGAATTCTCACGTATGCCAACCCGTAATGTGGTTTCTTGGACCGCCATCATTTCTGGTTTTGTGCATAAGGACGATTCTATTTCTGCCGTCAAATTTTTCAGAGAAATGAGAAAAATCGGAGAGCAAATGAATAAGTACACTGTTACGAGTGTACTACTGCATGTGCTAAACCATCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0080156 GO:0090304 GO:0090305 GO:0097159 GO:1900864 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

267

Amino Acids

29.75

Weight (kDa)

7.07

Isoelectric Point (pI)

34.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	14 - 61	1.1e-08	PPR repeat family
PPR_2	PF13041	116 - 163	1.4e-10	PPR repeat family
PPR_2	PF13041	215 - 259	1.6e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012277)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74600
fragaria_vesca	FvH4_5g15030
malus_domestica	MD06G1192600.v1.1 MD14G1199400.v1.1
prunus_persica	Prupe.5G192400_v2.0.a1 Prupe.5G192400_v2.0.a1 Prupe.5G192400_v2.0.a1
pyrus_communis	pycom06g17070 pycom14g16530
rosa_chinensis	RchiOBHm_Chr7g0181221
rosa_laevigata	RLG00000005205
rosa_multiflora	Rmu_sc0001147.1_g000014
rosa_roxburghii	Rroxscaffold_3G00272190
rosa_rugosa	Rorug06G0439600
rosa_samantha	Rh7AG041400 Rh7BG040700 Rh7CG042900 Rh7DG041200
rosa_wichuraiana	Rw7G003410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	78
Acc36I	ACCTGC	1 cut(s)	205
AccII	CGCG	2 cut(s)	290, 543
AciI	CCGC	3 cut(s)	480, 543, 663
AcsI	RAATTY	2 cut(s)	623, 713
AfaI	GTAC	2 cut(s)	758, 774
AfiI	CCNNNNNNNGG	2 cut(s)	206, 649
AgsI	TTSAA	4 cut(s)	308, 497, 506, 524
AjuI	GAANNNNNNNTTGG	2 cut(s)	492, 524
AluBI	AGCT	4 cut(s)	19, 197, 303, 614
AluI	AGCT	4 cut(s)	19, 197, 303, 614
Ama87I	CYCGRG	1 cut(s)	14
AoxI	GGCC	2 cut(s)	143, 377
ApaI	GGGCCC	1 cut(s)	147
ApeKI	GCWGC	2 cut(s)	300, 488
ApoI	RAATTY	2 cut(s)	623, 713
AspS9I	GGNCC	4 cut(s)	143, 144, 377, 660
AsuHPI	GGTGA	1 cut(s)	544
AsuII	TTCGAA	1 cut(s)	251
AvaI	CYCGRG	1 cut(s)	14
AvaII	GGWCC	1 cut(s)	660
BaeGI	GKGCMC	1 cut(s)	147
BanII	GRGCYC	1 cut(s)	147
BbvI	GCAGC	2 cut(s)	312, 475
BccI	CCATC	1 cut(s)	674
BceAI	ACGGC	1 cut(s)	692
BcgI	CGANNNNNNTGC	4 cut(s)	137, 171, 401, 435
BfaI	CTAG	1 cut(s)	468
BfuAI	ACCTGC	1 cut(s)	205
BisI	GCNGC	2 cut(s)	301, 489
BlsI	GCNGC	2 cut(s)	302, 490
Bme18I	GGWCC	1 cut(s)	660
BmeT110I	CYCGRG	1 cut(s)	14
BmgT120I	GGNCC	4 cut(s)	143, 144, 377, 660
BmiI	GGNNCC	1 cut(s)	145
BmsI	GCATC	3 cut(s)	114, 301, 421
Bpu14I	TTCGAA	1 cut(s)	251
BsaAI	YACGTR	1 cut(s)	632
BsaWI	WCCGGW	1 cut(s)	71
Bsc4I	CCNNNNNNNGG	2 cut(s)	206, 649
Bse3DI	GCAATG	2 cut(s)	183, 237
BseGI	GGATG	1 cut(s)	793
BseLI	CCNNNNNNNGG	2 cut(s)	206, 649
BseMI	GCAATG	2 cut(s)	183, 237
BseSI	GKGCMC	1 cut(s)	147
BseXI	GCAGC	2 cut(s)	312, 475
BsgI	GTGCAG	1 cut(s)	474
Bsh1236I	CGCG	2 cut(s)	290, 543
BshFI	GGCC	2 cut(s)	145, 379
BsiHKCI	CYCGRG	1 cut(s)	14
BsiSI	CCGG	1 cut(s)	72
BslFI	GGGAC	1 cut(s)	580
BslI	CCNNNNNNNGG	2 cut(s)	206, 649
BsmFI	GGGAC	1 cut(s)	580
BsmI	GAATGC	1 cut(s)	367
BsnI	GGCC	2 cut(s)	145, 379
BsoBI	CYCGRG	1 cut(s)	14
Bsp119I	TTCGAA	1 cut(s)	251
Bsp120I	GGGCCC	1 cut(s)	143
Bsp1286I	GDGCHC	1 cut(s)	147
Bsp68I	TCGCGA	1 cut(s)	290
BspACI	CCGC	3 cut(s)	480, 543, 663
BspANI	GGCC	2 cut(s)	145, 379
BspFNI	CGCG	2 cut(s)	290, 543
BspHI	TCATGA	2 cut(s)	63, 328
BspLI	GGNNCC	1 cut(s)	145
BspMI	ACCTGC	1 cut(s)	205
BspT104I	TTCGAA	1 cut(s)	251
BsrDI	GCAATG	2 cut(s)	183, 237
Bst4CI	ACNGT	1 cut(s)	763
BstBAI	YACGTR	1 cut(s)	632
BstBI	TTCGAA	1 cut(s)	251
BstC8I	GCNNGC	4 cut(s)	129, 181, 195, 228
BstF5I	GGATG	1 cut(s)	793
BstFNI	CGCG	2 cut(s)	290, 543
BstMWI	GCNNNNNNNGC	3 cut(s)	185, 194, 488
BstNSI	RCATGY	2 cut(s)	131, 785
BstSLI	GKGCMC	1 cut(s)	147
BstUI	CGCG	2 cut(s)	290, 543
BstV1I	GCAGC	2 cut(s)	312, 475
BsuRI	GGCC	2 cut(s)	145, 379
BtsCI	GGATG	1 cut(s)	793
BtsIMutI	CAGTG	1 cut(s)	759
BtuMI	TCGCGA	1 cut(s)	290
BveI	ACCTGC	1 cut(s)	205
Cac8I	GCNNGC	4 cut(s)	129, 181, 195, 228
CciI	TCATGA	2 cut(s)	63, 328
Cfr13I	GGNCC	4 cut(s)	143, 144, 377, 660
Csp6I	GTAC	2 cut(s)	757, 773
CviAII	CATG	8 cut(s)	54, 64, 105, 128, 329, 339, 782, 798
CviJI	RGCY	7 cut(s)	19, 145, 179, 197, 303, 379, 614
CviKI_1	RGCY	7 cut(s)	19, 145, 179, 197, 303, 379, 614
CviQI	GTAC	2 cut(s)	757, 773
Eco24I	GRGCYC	1 cut(s)	147
Eco47I	GGWCC	1 cut(s)	660
Eco88I	CYCGRG	1 cut(s)	14
EcoRI	GAATTC	1 cut(s)	623
EcoT22I	ATGCAT	1 cut(s)	129
EcoT38I	GRGCYC	1 cut(s)	147
FaeI	CATG	8 cut(s)	57, 67, 108, 131, 332, 342, 785, 801
FaqI	GGGAC	1 cut(s)	580
FatI	CATG	8 cut(s)	53, 63, 104, 127, 328, 338, 781, 797
Fnu4HI	GCNGC	2 cut(s)	301, 489
FokI	GGATG	1 cut(s)	780
FriOI	GRGCYC	1 cut(s)	147
Fsp4HI	GCNGC	2 cut(s)	301, 489
FspBI	CTAG	1 cut(s)	468
GluI	GCNGC	2 cut(s)	301, 489
HaeIII	GGCC	2 cut(s)	145, 379
HapII	CCGG	1 cut(s)	72
Hin1II	CATG	8 cut(s)	57, 67, 108, 131, 332, 342, 785, 801
HinfI	GANTC	2 cut(s)	438, 695
HpaII	CCGG	1 cut(s)	72
HphI	GGTGA	1 cut(s)	544
Hpy166II	GTNNAC	2 cut(s)	759, 773
Hpy188I	TCNGA	4 cut(s)	123, 423, 722, 740
Hpy188III	TCNNGA	5 cut(s)	64, 97, 270, 289, 329
Hpy8I	GTNNAC	2 cut(s)	759, 773
HpyAV	CCTTC	2 cut(s)	94, 508
HpyCH4III	ACNGT	1 cut(s)	763
HpyCH4IV	ACGT	1 cut(s)	631
HpyF10VI	GCNNNNNNNGC	3 cut(s)	185, 194, 488
HpySE526I	ACGT	1 cut(s)	631
Hsp92II	CATG	8 cut(s)	57, 67, 108, 131, 332, 342, 785, 801
LmnI	GCTCC	1 cut(s)	202
LpnPI	CCDG	7 cut(s)	85, 200, 255, 360, 385, 393, 660
Lsp1109I	GCAGC	2 cut(s)	312, 475
LweI	GCATC	3 cut(s)	114, 301, 421
MaeI	CTAG	1 cut(s)	468
MaeII	ACGT	1 cut(s)	631
MaeIII	GTNAC	2 cut(s)	341, 763
MboII	GAAGA	2 cut(s)	320, 509
MhlI	GDGCHC	1 cut(s)	147
MluCI	AATT	5 cut(s)	30, 295, 501, 623, 713
MnlI	CCTC	2 cut(s)	312, 452
Mph1103I	ATGCAT	1 cut(s)	129
MseI	TTAA	3 cut(s)	29, 476, 537
MspI	CCGG	1 cut(s)	72
Mva1269I	GAATGC	1 cut(s)	367
MvnI	CGCG	2 cut(s)	290, 543
MwoI	GCNNNNNNNGC	3 cut(s)	185, 194, 488
NlaIII	CATG	8 cut(s)	57, 67, 108, 131, 332, 342, 785, 801
NlaIV	GGNNCC	1 cut(s)	145
NmuCI	GTSAC	1 cut(s)	341
NruI	TCGCGA	1 cut(s)	290
NsiI	ATGCAT	1 cut(s)	129
NspI	RCATGY	2 cut(s)	131, 785
NspV	TTCGAA	1 cut(s)	251
PaeI	GCATGC	1 cut(s)	131
PaeR7I	CTCGAG	1 cut(s)	14
PagI	TCATGA	2 cut(s)	63, 328
PctI	GAATGC	1 cut(s)	367
PfeI	GAWTC	2 cut(s)	438, 695
PkrI	GCNGC	2 cut(s)	302, 490
Ppu21I	YACGTR	1 cut(s)	632
PsiI	TTATAA	1 cut(s)	78
PspN4I	GGNNCC	1 cut(s)	145
PspOMI	GGGCCC	1 cut(s)	143
PspPI	GGNCC	4 cut(s)	143, 144, 377, 660
PspXI	VCTCGAGB	1 cut(s)	14
RruI	TCGCGA	1 cut(s)	290
RsaI	GTAC	2 cut(s)	758, 774
RsaNI	GTAC	2 cut(s)	757, 773
SaqAI	TTAA	3 cut(s)	29, 476, 537
SatI	GCNGC	2 cut(s)	301, 489
Sau96I	GGNCC	4 cut(s)	143, 144, 377, 660
SduI	GDGCHC	1 cut(s)	147
SetI	ASST	6 cut(s)	21, 199, 219, 305, 616, 634
SfaNI	GCATC	3 cut(s)	114, 301, 421
Sfr274I	CTCGAG	1 cut(s)	14
SfuI	TTCGAA	1 cut(s)	251
SinI	GGWCC	1 cut(s)	660
SlaI	CTCGAG	1 cut(s)	14
SmlI	CTYRAG	1 cut(s)	14
SmoI	CTYRAG	1 cut(s)	14
SphI	GCATGC	1 cut(s)	131
Sse9I	AATT	5 cut(s)	30, 295, 501, 623, 713
SsiI	CCGC	3 cut(s)	480, 543, 663
SspMI	CTAG	1 cut(s)	468
TaaI	ACNGT	1 cut(s)	763
TaiI	ACGT	1 cut(s)	634
TaqI	TCGA	3 cut(s)	15, 96, 251
TaqII	GACCGA	1 cut(s)	54
TasI	AATT	5 cut(s)	30, 295, 501, 623, 713
TatI	WGTACW	2 cut(s)	756, 772
TfiI	GAWTC	2 cut(s)	438, 695
Tru1I	TTAA	3 cut(s)	29, 476, 537
Tru9I	TTAA	3 cut(s)	29, 476, 537
TscAI	CASTG	1 cut(s)	766
TseFI	GTSAC	1 cut(s)	341
TseI	GCWGC	2 cut(s)	300, 488
Tsp45I	GTSAC	1 cut(s)	341
TspDTI	ATGAA	7 cut(s)	42, 248, 317, 327, 620, 624, 764
TspRI	CASTG	1 cut(s)	766
VpaK11BI	GGWCC	1 cut(s)	660
XapI	RAATTY	2 cut(s)	623, 713
XceI	RCATGY	2 cut(s)	131, 785
XhoI	CTCGAG	1 cut(s)	14
XspI	CTAG	1 cut(s)	468
Zsp2I	ATGCAT	1 cut(s)	129

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.