Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0010033

Physical Location & Seq

Genomic Coordinates Reverse (-)

29681024 .. 29684280

Gene Structure 3257 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1211500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1785 bp

ATGGCGGCGTCGGACAGGCGGTGCCACTACGAGGTTTTAGGTCTTGCCCGCGACTGCTCCGCCGACGAGATCCGGTCGGCTTACAGGAAGCTCGCTCTCCAGCGCCACCCGGACAAGCTAGTCCAGTCCGGCTTATCCCAGTCCGAAGCCACGGCCCAGTTCCAGGAGCTGGCCCATGCCTACGAGGTTCTCTCCGACCCCAAAGAGCGAGCTTGGTACGACTCTCACCGCTCCCAAATCCTCTTCTCCGATCGCAGATCGGCCGCGTCGGGCTCCGGCTCGGGCATCCCCGATCTCTTCTCATTCTTCTCCACCACCGTCTTCTCTGGGTACTCCGACTCCGGCCGCGGATTTTATAAGGTATATTCCGATGTCTTCAACAAAATCTACGCCAACGAGCTCAATTTTGCTAGGAAATTAGGATTAGGGTTGGACACCGTGGCTGAAGCTCCGGCGATGGGGAATTTAGAGAGTCCGTACGTGCAGGTCACCGCGTTTTATAACTACTGGGCAGGGTTTTGTACGGTGATGGATTTTTGCTGGGAGGACAAGTATGACGTGATGGCCGGTCCTAACCGGAAGTCGAGGAGGTTGATGGAGGAGGAGAACAAGAAGGAGAGGAAGAAGGCTAAGAGAGAGTTCAACGAGACGGTGCGGGGGTTGGTGGATTTTGTGAAGAAGAGGGACAAGAGGGTGATTGATATGATGCTGAAGAAGGAGGAGGAGAGGGTGAGGAAGAGGGAGGAGGAGAGAGAGAGGAAGAAGAAGTTGGAGAAGGAGAAATTGGAGAGGGCTATGGCGTATGAGGAGCCGGAGTGGGCGAAAGTGGAGGAGGAGGAAGGAGGGAATGGGGTTGAGGAATTGGAGGAGGACGAGGAGGAGCAGATGAGGAAGGAATTCTATTGTGTAGTGTGTGGGAAGAAGTTTAAGAGTGAGAAGCAGTGGAAGAATCACGAGCAGTCGAAGAAGCATCGAGATAAGGTTGCAGAGTATCGGGAGTCGATTGGAGAAGAAGATCTTGAACTTGATGATGAAGAGGTTTTGGAGGGAGAGGGTGAAGAAAGGGGTGAGCTTGGCGATGTTGATGAATTAGGTCAGGAGATTAGAGAGGGGTTGAAGATTGGGGAGGAGGAAGATGGGGCAGGAGTGAGTGATCAGGAGGATGAGTTGCACGAATTTGGTGGTGAAAATGGGAATGAAAAGGTTGACGAGGCACTTGGGTTGGATGGTGATGAAGATGAAGATGAAATGGGTGTTCTTGAAGCAATGGTGGCCGGGCGAAAGATTAAGAAAAATGTAGCCTTTTGGGTTGAGCGTGAGGATAAGGATGACGATGACGCGGAGTTCATGGAATATAATAACCGGAAGAGCGCAAGAAGGAAAGGAGGAGCGAGGAAAGAGAGGAGTAAGAAGAGTGGTGGTGAAGCTAATAATGTTGATAAAAGTGGAAACAACGGTGGTCAGAGTGAGGAGAGTAATGAACAAGATAATTCAAATATGAAGGAATCCACATCTCATTCCGTTGTGGAAAATGAGAGTACTGATAAAGGGGATGAACATTTAGCACGAAAGAAGAAGAGCTCAAGCAAAGCCGTCGATAAGAAAGAGAAAGTGAAGAAAGTGGCGAACGACAAATTAAAGAATTCATGTAATGGGAAGAAATCTAAGGGAGCATTGAAGAATACTAGCAACGCATGTGATACATGTGGAGAGGAGTTCGAATCAAGAAACAAGTTACATATGCATTTGGGTGACACTGGACATGCGAAGCTGAAGTATCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DnaJ zf-C2H2_jaz

Protein Analysis

595

Amino Acids

67.82

Weight (kDa)

5.22

Isoelectric Point (pI)

42.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	9 - 74	6.7e-22	DnaJ domain
ZUO1-like_ZHD	PF21884	123 - 232	7.6e-23	Zuotin-like, zuotin homology domain
zf-C2H2_jaz	PF12171	300 - 325	6.3e-11	Zinc-finger double-stranded RNA-binding
zf-met	PF12874	300 - 324	1e-08	Zinc-finger of C2H2 type

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010033)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74250
fragaria_vesca	FvH4_5g13920
malus_domestica	MD06G1202400.v1.1 MD14G1211500.v1.1
prunus_persica	Prupe.5G204600_v2.0.a1 Prupe.5G204600_v2.0.a1 Prupe.5G204600_v2.0.a1
pyrus_communis	pycom06g17840 pycom14g17510
rosa_chinensis	RchiOBHm_Chr7g0183041
rosa_laevigata	RLG00000005085
rosa_multiflora	Rmu_sc0000014.1_g000005 Rmu_sc0021698.1_g000001 Rmu_ssc0000111.1_g000001
rosa_roxburghii	Rroxscaffold_3G00270820
rosa_rugosa	Rorug06G0452000
rosa_samantha	Rh7AG055500 Rh7BG054900 Rh7CG056200 Rh7DG055000
rosa_wichuraiana	Rw7G004510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	357, 501
AasI	GACNNNNNNGTC	1 cut(s)	119
Acc36I	ACCTGC	1 cut(s)	475
AccB1I	GGYRCC	1 cut(s)	21
AccB7I	CCANNNNNTGG	1 cut(s)	169
AccBSI	CCGCTC	1 cut(s)	231
AccII	CGCG	5 cut(s)	51, 266, 348, 494, 1340
AclWI	GGATC	1 cut(s)	64
AcoI	YGGCCR	4 cut(s)	261, 343, 564, 1272
AcsI	RAATTY	4 cut(s)	463, 896, 1175, 1642
AcuI	CTGAAG	2 cut(s)	465, 731
AcyI	GRCGYC	1 cut(s)	8
AfaI	GTAC	5 cut(s)	218, 332, 479, 523, 1540
AfiI	CCNNNNNNNGG	4 cut(s)	31, 163, 169, 458
AflIII	ACRYGT	1 cut(s)	1703
AgsI	TTSAA	7 cut(s)	379, 643, 1022, 1117, 1262, 1494, 1678
AjiI	CACGTC	1 cut(s)	559
AjnI	CCWGG	1 cut(s)	162
AjuI	GAANNNNNNNTTGG	4 cut(s)	752, 767, 784, 799
Alw21I	GWGCWC	2 cut(s)	402, 1583
Alw26I	GTCTC	1 cut(s)	641
AlwI	GGATC	1 cut(s)	64
AlwNI	CAGNNNCTG	1 cut(s)	169
Ama87I	CYCGRG	1 cut(s)	280
AoxI	GGCC	6 cut(s)	153, 171, 261, 343, 564, 1272
ApoI	RAATTY	4 cut(s)	463, 896, 1175, 1642
Asp700I	GAANNNNTTC	1 cut(s)	896
AspLEI	GCGC	2 cut(s)	105, 1373
AspS9I	GGNCC	3 cut(s)	154, 172, 569
AsuC2I	CCSGG	2 cut(s)	110, 1276
AsuII	TTCGAA	1 cut(s)	1719
AvaI	CYCGRG	1 cut(s)	280
AvaII	GGWCC	1 cut(s)	569
BanI	GGYRCC	1 cut(s)	21
BanII	GRGCYC	3 cut(s)	275, 402, 1583
BauI	CACGAG	1 cut(s)	953
BbsI	GAAGAC	2 cut(s)	313, 367
Bbv12I	GWGCWC	2 cut(s)	402, 1583
BccI	CCATC	6 cut(s)	451, 523, 556, 589, 1130, 1220
BceAI	ACGGC	2 cut(s)	168, 1577
BcgI	CGANNNNNNTGC	2 cut(s)	1576, 1610
BciT130I	CCWGG	1 cut(s)	164
BclI	TGATCA	1 cut(s)	1153
BcnI	CCSGG	2 cut(s)	110, 1276
BcoDI	GTCTC	1 cut(s)	641
BfaI	CTAG	3 cut(s)	119, 411, 1686
BfoI	RGCGCY	1 cut(s)	106
BfuAI	ACCTGC	1 cut(s)	475
BglII	AGATCT	1 cut(s)	1015
BisI	GCNGC	3 cut(s)	6, 264, 346
BlsI	GCNGC	3 cut(s)	7, 265, 347
BmcAI	AGTACT	1 cut(s)	1540
Bme1390I	CCNGG	3 cut(s)	110, 164, 1276
Bme18I	GGWCC	1 cut(s)	569
BmeT110I	CYCGRG	1 cut(s)	280
BmgBI	CACGTC	1 cut(s)	559
BmgT120I	GGNCC	3 cut(s)	154, 172, 569
BmiI	GGNNCC	3 cut(s)	23, 274, 810
BmrFI	CCNGG	3 cut(s)	110, 164, 1276
BmrI	ACTGGG	3 cut(s)	133, 151, 517
BmsI	GCATC	3 cut(s)	294, 696, 979
BmuI	ACTGGG	3 cut(s)	133, 151, 517
BpiI	GAAGAC	2 cut(s)	313, 367
BplI	GAGNNNNNCTC	2 cut(s)	176, 208
BpmI	CTGGAG	1 cut(s)	83
Bpu14I	TTCGAA	1 cut(s)	1719
BpuEI	CTTGAG	1 cut(s)	1567
BpuMI	CCSGG	2 cut(s)	110, 1276
Bsa29I	ATCGAT	1 cut(s)	1780
BsaAI	YACGTR	1 cut(s)	481
BsaHI	GRCGYC	1 cut(s)	8
BsaJI	CCNNGG	3 cut(s)	150, 346, 438
BsaWI	WCCGGW	3 cut(s)	72, 576, 1362
BsaXI	ACNNNNNCTCC	8 cut(s)	323, 353, 536, 566, 800, 830, 1151, 1181
Bsc4I	CCNNNNNNNGG	4 cut(s)	31, 163, 169, 458
Bse118I	RCCGGY	1 cut(s)	566
Bse1I	ACTGG	5 cut(s)	124, 139, 157, 512, 1762
Bse3DI	GCAATG	1 cut(s)	1272
BseBI	CCWGG	1 cut(s)	164
BseCI	ATCGAT	1 cut(s)	1780
BseDI	CCNNGG	3 cut(s)	150, 346, 438
BseGI	GGATG	5 cut(s)	285, 1168, 1231, 1333, 1558
BseLI	CCNNNNNNNGG	4 cut(s)	31, 163, 169, 458
BseMI	GCAATG	1 cut(s)	1272
BseNI	ACTGG	5 cut(s)	124, 139, 157, 512, 1762
BseX3I	CGGCCG	2 cut(s)	261, 343
BseYI	CCCAGC	1 cut(s)	540
BsgI	GTGCAG	1 cut(s)	503
Bsh1236I	CGCG	5 cut(s)	51, 266, 348, 494, 1340
Bsh1285I	CGRYCG	4 cut(s)	77, 253, 264, 346
BshFI	GGCC	6 cut(s)	155, 173, 263, 345, 566, 1274
BshNI	GGYRCC	1 cut(s)	21
BshVI	ATCGAT	1 cut(s)	1780
BsiEI	CGRYCG	4 cut(s)	77, 253, 264, 346
BsiHKAI	GWGCWC	2 cut(s)	402, 1583
BsiHKCI	CYCGRG	1 cut(s)	280
BsiWI	CGTACG	1 cut(s)	477
BslFI	GGGAC	1 cut(s)	698
BslI	CCNNNNNNNGG	4 cut(s)	31, 163, 169, 458
BsmAI	GTCTC	1 cut(s)	641
BsmBI	CGTCTC	1 cut(s)	641
BsmFI	GGGAC	1 cut(s)	698
BsnI	GGCC	6 cut(s)	155, 173, 263, 345, 566, 1274
BsoBI	CYCGRG	1 cut(s)	280
Bsp119I	TTCGAA	1 cut(s)	1719
Bsp1286I	GDGCHC	3 cut(s)	275, 402, 1583
Bsp143I	GATC	6 cut(s)	69, 250, 257, 292, 1015, 1153
BspANI	GGCC	6 cut(s)	155, 173, 263, 345, 566, 1274
BspDI	ATCGAT	1 cut(s)	1780
BspFNI	CGCG	5 cut(s)	51, 266, 348, 494, 1340
BspLI	GGNNCC	3 cut(s)	23, 274, 810
BspMI	ACCTGC	1 cut(s)	475
BspPI	GGATC	1 cut(s)	64
BspQI	GCTCTTC	2 cut(s)	1361, 1571
BspT104I	TTCGAA	1 cut(s)	1719
BspT107I	GGYRCC	1 cut(s)	21
BsrBI	CCGCTC	1 cut(s)	231
BsrDI	GCAATG	1 cut(s)	1272
BsrFI	RCCGGY	1 cut(s)	566
BsrI	ACTGG	5 cut(s)	124, 139, 157, 512, 1762
BssAI	RCCGGY	1 cut(s)	566
BssECI	CCNNGG	3 cut(s)	150, 346, 438
BssMI	GATC	6 cut(s)	69, 250, 257, 292, 1015, 1153
BssNI	GRCGYC	1 cut(s)	8
BssSI	CACGAG	1 cut(s)	953
Bst2BI	CACGAG	1 cut(s)	953
Bst2UI	CCWGG	1 cut(s)	164
Bst4CI	ACNGT	5 cut(s)	319, 439, 526, 652, 1457
Bst6I	CTCTTC	8 cut(s)	248, 302, 674, 731, 1029, 1361, 1406, 1571
BstACI	GRCGYC	1 cut(s)	8
BstBAI	YACGTR	1 cut(s)	481
BstBI	TTCGAA	1 cut(s)	1719
BstC8I	GCNNGC	4 cut(s)	49, 93, 171, 210
BstDEI	CTNAG	2 cut(s)	630, 1665
BstDSI	CCRYGG	3 cut(s)	150, 346, 438
BstEII	GGTNACC	1 cut(s)	487
BstF5I	GGATG	5 cut(s)	285, 1168, 1231, 1333, 1558
BstFNI	CGCG	5 cut(s)	51, 266, 348, 494, 1340
BstH2I	RGCGCY	1 cut(s)	106
BstHHI	GCGC	2 cut(s)	105, 1373
BstKTI	GATC	6 cut(s)	72, 253, 260, 295, 1018, 1156
BstMAI	GTCTC	1 cut(s)	641
BstMBI	GATC	6 cut(s)	69, 250, 257, 292, 1015, 1153
BstMCI	CGRYCG	4 cut(s)	77, 253, 264, 346
BstMWI	GCNNNNNNNGC	1 cut(s)	1271
BstNI	CCWGG	1 cut(s)	164
BstNSI	RCATGY	3 cut(s)	1698, 1707, 1766
BstPI	GGTNACC	1 cut(s)	487
BstSCI	CCNGG	3 cut(s)	108, 162, 1274
BstUI	CGCG	5 cut(s)	51, 266, 348, 494, 1340
BstV2I	GAAGAC	2 cut(s)	313, 367
BstX2I	RGATCY	2 cut(s)	69, 1015
BstYI	RGATCY	2 cut(s)	69, 1015
BstZI	CGGCCG	2 cut(s)	261, 343
Bsu15I	ATCGAT	1 cut(s)	1780
BsuRI	GGCC	6 cut(s)	155, 173, 263, 345, 566, 1274
BsuTUI	ATCGAT	1 cut(s)	1780
BtgI	CCRYGG	3 cut(s)	150, 346, 438
BtgZI	GCGATG	2 cut(s)	470, 1092
BtrI	CACGTC	1 cut(s)	559
BtsCI	GGATG	5 cut(s)	285, 1168, 1231, 1333, 1558
BtsI	GCAGTG	1 cut(s)	947
BtsIMutI	CAGTG	2 cut(s)	947, 1755
BveI	ACCTGC	1 cut(s)	475
Cac8I	GCNNGC	4 cut(s)	49, 93, 171, 210
CaiI	CAGNNNCTG	1 cut(s)	169
CfoI	GCGC	2 cut(s)	105, 1373
Cfr10I	RCCGGY	1 cut(s)	566
Cfr13I	GGNCC	3 cut(s)	154, 172, 569
Cfr42I	CCGCGG	1 cut(s)	349
ClaI	ATCGAT	1 cut(s)	1780
CseI	GACGC	2 cut(s)	255, 1346
Csp6I	GTAC	5 cut(s)	217, 331, 478, 522, 1539
CviAII	CATG	6 cut(s)	176, 1348, 1647, 1695, 1704, 1763
CviQI	GTAC	5 cut(s)	217, 331, 478, 522, 1539
DdeI	CTNAG	2 cut(s)	630, 1665
DpnI	GATC	6 cut(s)	71, 252, 259, 294, 1017, 1155
DpnII	GATC	6 cut(s)	69, 250, 257, 292, 1015, 1153
DrdI	GACNNNNNNGTC	1 cut(s)	119
DseDI	GACNNNNNNGTC	1 cut(s)	119
EaeI	YGGCCR	4 cut(s)	261, 343, 564, 1272
EagI	CGGCCG	2 cut(s)	261, 343
Eam1104I	CTCTTC	8 cut(s)	248, 302, 674, 731, 1029, 1361, 1406, 1571
EarI	CTCTTC	8 cut(s)	248, 302, 674, 731, 1029, 1361, 1406, 1571
EciI	GGCGGA	1 cut(s)	49
Ecl136II	GAGCTC	2 cut(s)	400, 1581
EclXI	CGGCCG	2 cut(s)	261, 343
Eco24I	GRGCYC	3 cut(s)	275, 402, 1583
Eco47I	GGWCC	1 cut(s)	569
Eco52I	CGGCCG	2 cut(s)	261, 343
Eco53kI	GAGCTC	2 cut(s)	400, 1581
Eco57I	CTGAAG	2 cut(s)	465, 731
Eco88I	CYCGRG	1 cut(s)	280
Eco91I	GGTNACC	1 cut(s)	487
EcoICRI	GAGCTC	2 cut(s)	400, 1581
EcoO65I	GGTNACC	1 cut(s)	487
EcoRI	GAATTC	2 cut(s)	896, 1642
EcoRII	CCWGG	1 cut(s)	162
EcoT22I	ATGCAT	1 cut(s)	1746
EcoT38I	GRGCYC	3 cut(s)	275, 402, 1583
Esp3I	CGTCTC	1 cut(s)	641
FaeI	CATG	6 cut(s)	179, 1351, 1650, 1698, 1707, 1766
FalI	AAGNNNNNCTT	2 cut(s)	1002, 1034
FaqI	GGGAC	1 cut(s)	698
FatI	CATG	6 cut(s)	175, 1347, 1646, 1694, 1703, 1762
FauI	CCCGC	2 cut(s)	56, 648
FauNDI	CATATG	1 cut(s)	1740
FbaI	TGATCA	1 cut(s)	1153
Fnu4HI	GCNGC	3 cut(s)	6, 264, 346
FokI	GGATG	5 cut(s)	272, 1175, 1238, 1340, 1565
FriOI	GRGCYC	3 cut(s)	275, 402, 1583
Fsp4HI	GCNGC	3 cut(s)	6, 264, 346
FspBI	CTAG	3 cut(s)	119, 411, 1686
GlaI	GCGC	2 cut(s)	104, 1372
GluI	GCNGC	3 cut(s)	6, 264, 346
GsaI	CCCAGC	1 cut(s)	544
GsuI	CTGGAG	1 cut(s)	83
HaeII	RGCGCY	1 cut(s)	106
HaeIII	GGCC	6 cut(s)	155, 173, 263, 345, 566, 1274
HgaI	GACGC	2 cut(s)	255, 1346
HhaI	GCGC	2 cut(s)	105, 1373
Hin1I	GRCGYC	1 cut(s)	8
Hin1II	CATG	6 cut(s)	179, 1351, 1650, 1698, 1707, 1766
Hin6I	GCGC	2 cut(s)	103, 1371
HinP1I	GCGC	2 cut(s)	103, 1371
HincII	GTYRAC	1 cut(s)	1207
HindII	GTYRAC	1 cut(s)	1207
HinfI	GANTC	7 cut(s)	221, 338, 472, 949, 998, 1505, 1721
Hpy166II	GTNNAC	1 cut(s)	1207
Hpy188I	TCNGA	7 cut(s)	13, 145, 196, 250, 337, 370, 1464
Hpy188III	TCNNGA	8 cut(s)	953, 974, 995, 1019, 1097, 1157, 1259, 1725
Hpy8I	GTNNAC	1 cut(s)	1207
Hpy99I	CGWCG	4 cut(s)	13, 68, 271, 1598
HpyAV	CCTTC	8 cut(s)	607, 619, 709, 769, 833, 886, 1371, 1495
HpyCH4III	ACNGT	5 cut(s)	319, 439, 526, 652, 1457
HpyCH4IV	ACGT	2 cut(s)	480, 558
HpyCH4V	TGCA	4 cut(s)	484, 986, 1171, 1744
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1271
HpyF3I	CTNAG	2 cut(s)	630, 1665
HpySE526I	ACGT	2 cut(s)	480, 558
Hsp92I	GRCGYC	1 cut(s)	8
Hsp92II	CATG	6 cut(s)	179, 1351, 1650, 1698, 1707, 1766
HspAI	GCGC	2 cut(s)	103, 1371
Ksp22I	TGATCA	1 cut(s)	1153
KspI	CCGCGG	1 cut(s)	349
Kzo9I	GATC	6 cut(s)	69, 250, 257, 292, 1015, 1153
LguI	GCTCTTC	2 cut(s)	1361, 1571
LmnI	GCTCC	9 cut(s)	62, 166, 236, 278, 454, 808, 880, 1388, 1670
LweI	GCATC	3 cut(s)	294, 696, 979
MaeI	CTAG	3 cut(s)	119, 411, 1686
MaeII	ACGT	2 cut(s)	480, 558
MaeIII	GTNAC	3 cut(s)	487, 1734, 1751
MalI	GATC	6 cut(s)	71, 252, 259, 294, 1017, 1155
MbiI	CCGCTC	1 cut(s)	231
MboI	GATC	6 cut(s)	69, 250, 257, 292, 1015, 1153
MflI	RGATCY	2 cut(s)	69, 1015
MhlI	GDGCHC	3 cut(s)	275, 402, 1583
MlyI	GAGTC	4 cut(s)	215, 332, 481, 1007
MmeI	TCCRAC	5 cut(s)	219, 360, 411, 750, 1203
Mph1103I	ATGCAT	1 cut(s)	1746
MroXI	GAANNNNTTC	1 cut(s)	896
MseI	TTAA	3 cut(s)	927, 1287, 1637
MslI	CAYNNNNRTG	1 cut(s)	1749
MspA1I	CMGCKG	1 cut(s)	348
MspR9I	CCNGG	3 cut(s)	110, 164, 1276
MvaI	CCWGG	1 cut(s)	164
MvnI	CGCG	5 cut(s)	51, 266, 348, 494, 1340
MwoI	GCNNNNNNNGC	1 cut(s)	1271
NciI	CCSGG	2 cut(s)	110, 1276
NdeI	CATATG	1 cut(s)	1740
NdeII	GATC	6 cut(s)	69, 250, 257, 292, 1015, 1153
NlaIII	CATG	6 cut(s)	179, 1351, 1650, 1698, 1707, 1766
NlaIV	GGNNCC	3 cut(s)	23, 274, 810
NmuCI	GTSAC	2 cut(s)	487, 1751
NsiI	ATGCAT	1 cut(s)	1746
NspI	RCATGY	3 cut(s)	1698, 1707, 1766
NspV	TTCGAA	1 cut(s)	1719
PciI	ACATGT	1 cut(s)	1703
PciSI	GCTCTTC	2 cut(s)	1361, 1571
PdmI	GAANNNNTTC	1 cut(s)	896
PfeI	GAWTC	3 cut(s)	949, 1505, 1721
Pfl23II	CGTACG	1 cut(s)	477
PflMI	CCANNNNNTGG	1 cut(s)	169
PfoI	TCCNGGA	1 cut(s)	162
PkrI	GCNGC	3 cut(s)	7, 265, 347
Ple19I	CGATCG	1 cut(s)	253
PleI	GAGTC	4 cut(s)	215, 332, 480, 1006
PpsI	GAGTC	4 cut(s)	215, 332, 480, 1006
Ppu21I	YACGTR	1 cut(s)	481
PscI	ACATGT	1 cut(s)	1703
PsiI	TTATAA	2 cut(s)	357, 501
Psp124BI	GAGCTC	2 cut(s)	402, 1583
Psp6I	CCWGG	1 cut(s)	162
PspEI	GGTNACC	1 cut(s)	487
PspFI	CCCAGC	1 cut(s)	540
PspGI	CCWGG	1 cut(s)	162
PspLI	CGTACG	1 cut(s)	477
PspN4I	GGNNCC	3 cut(s)	23, 274, 810
PspPI	GGNCC	3 cut(s)	154, 172, 569
PstNI	CAGNNNCTG	1 cut(s)	169
PsuI	RGATCY	2 cut(s)	69, 1015
PvuI	CGATCG	1 cut(s)	253
RsaI	GTAC	5 cut(s)	218, 332, 479, 523, 1540
RsaNI	GTAC	5 cut(s)	217, 331, 478, 522, 1539
RseI	CAYNNNNRTG	1 cut(s)	1749
SacI	GAGCTC	2 cut(s)	402, 1583
SacII	CCGCGG	1 cut(s)	349
SapI	GCTCTTC	2 cut(s)	1361, 1571
SaqAI	TTAA	3 cut(s)	927, 1287, 1637
SatI	GCNGC	3 cut(s)	6, 264, 346
Sau3AI	GATC	6 cut(s)	69, 250, 257, 292, 1015, 1153
Sau96I	GGNCC	3 cut(s)	154, 172, 569
ScaI	AGTACT	1 cut(s)	1540
SchI	GAGTC	4 cut(s)	215, 332, 481, 1007
ScrFI	CCNGG	3 cut(s)	110, 164, 1276
SduI	GDGCHC	3 cut(s)	275, 402, 1583
SfaNI	GCATC	3 cut(s)	294, 696, 979
Sfr303I	CCGCGG	1 cut(s)	349
SfuI	TTCGAA	1 cut(s)	1719
SgrBI	CCGCGG	1 cut(s)	349
SinI	GGWCC	1 cut(s)	569
SmiMI	CAYNNNNRTG	1 cut(s)	1749
SmlI	CTYRAG	1 cut(s)	1582
SmoI	CTYRAG	1 cut(s)	1582
SspMI	CTAG	3 cut(s)	119, 411, 1686
SstI	GAGCTC	2 cut(s)	402, 1583
StyD4I	CCNGG	3 cut(s)	108, 162, 1274
TaaI	ACNGT	5 cut(s)	319, 439, 526, 652, 1457
TaiI	ACGT	2 cut(s)	483, 561
TaqI	TCGA	7 cut(s)	584, 962, 973, 1001, 1596, 1719, 1780
TatI	WGTACW	1 cut(s)	1538
TauI	GCSGC	3 cut(s)	8, 266, 348
TfiI	GAWTC	3 cut(s)	949, 1505, 1721
Tru1I	TTAA	3 cut(s)	927, 1287, 1637
Tru9I	TTAA	3 cut(s)	927, 1287, 1637
TscAI	CASTG	2 cut(s)	947, 1762
TseFI	GTSAC	2 cut(s)	487, 1751
Tsp45I	GTSAC	2 cut(s)	487, 1751
TspGWI	ACGGA	2 cut(s)	465, 1510
TspRI	CASTG	2 cut(s)	947, 1762
Van91I	CCANNNNNTGG	1 cut(s)	169
VpaK11BI	GGWCC	1 cut(s)	569
XapI	RAATTY	4 cut(s)	463, 896, 1175, 1642
XceI	RCATGY	3 cut(s)	1698, 1707, 1766
XmnI	GAANNNNTTC	1 cut(s)	896
XspI	CTAG	3 cut(s)	119, 411, 1686
ZrmI	AGTACT	1 cut(s)	1540
Zsp2I	ATGCAT	1 cut(s)	1746

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.