MD14G1235300.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005619
Physical Location & Seq
Forward (+)
31518057 .. 31520338
2282 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1235300.v1.1.491

Sequence Viewer

Length: 879 bp
ATGGGAAAAGTCTTGGATTCCAATTTCTTAGCTCTCACTGCTATTGTCACTGTGGGGTATCAGTTGTTGTTCTTCATAATCACTGCTCTTCTCAAGTTCGACAAAGTCACTGACTTTGCTGGAAGTACCAATTTTGTTATACTTGCTATTCTGACTTTGGTTGTGAAGGGGTCATGGCATTTTAGACAGGTAGTGTTGAGTTTGCTTGTGATAATATGGGGTCTTCGCCTCGGGTTGTTCCTACTGATGAGGATTTTGCAATGGGTAGAGGATCGGCGTTTTGATGAAATGCGTGGTAATATAGGGAAGTTAGCAGTTTTCTGGATATTTCAGGCTGTGTGGGTATGGACTGTGAGTTTACCTGTAACAGTTGCTAATGCAAGCAACAGAAAACCATCACTCCAAGCCGCGGACATAATTGGATGGGTTATATGGTTTGTCGGTTTTTCAGTTGAAGCTGCAGCGGATCAGCAAAAACTGACATTCAAAGGTTCACCACAAAATAGAGGAAAGTGGTGCAATGTCGGACTATGGAAATACACTCGCCATCCAAACTATTTTGGTGAGATATTCCTTTGGTGGGGAGTGTTTATTGCTTCCACGCCTGTATTGAAAGGTGCAGAGTGGCTGGTGATTTTTGGACCGATCTTTCTCACGTTGTTGCTTCTTTTCATCAGTGGCATACCGATGCTTGAGGACTCTGCAGACAAGAAGTTTGGAAACGTGGACGAATACAGGCTGTATAAAAGATCGACTAGCCCTCTGATTCCGCTACCTCCGGCTATATACAAGAACTTGCCATTGTGGTTCAAATCGACTTTTCTCTTTGAGTTTCCTCTCTACAGTCGTAATCTTCCTCGGGAAGAGCCAAACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1295

Protein Analysis

293

Amino Acids

33.58

Weight (kDa)

9.65

Isoelectric Point (pI)

26.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1295 PF06966 24 - 252 2e-89 Protein of unknown function (DUF1295)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 410
AciI CCGC 4 cut(s) 408, 410, 464, 770
AclWI GGATC 2 cut(s) 279, 474
AfaI GTAC 1 cut(s) 127
AfiI CCNNNNNNNGG 2 cut(s) 409, 580
AgsI TTSAA 4 cut(s) 455, 487, 613, 811
AluBI AGCT 2 cut(s) 32, 458
AluI AGCT 2 cut(s) 32, 458
AlwI GGATC 2 cut(s) 279, 474
Ama87I CYCGRG 2 cut(s) 230, 858
ApeKI GCWGC 2 cut(s) 458, 461
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
AspS9I GGNCC 1 cut(s) 641
AsuHPI GGTGA 3 cut(s) 486, 575, 643
AvaI CYCGRG 2 cut(s) 230, 858
AvaII GGWCC 1 cut(s) 641
BbsI GAAGAC 1 cut(s) 215
BbvI GCAGC 2 cut(s) 445, 473
BccI CCATC 3 cut(s) 403, 417, 555
BfaI CTAG 1 cut(s) 756
BfmI CTRYAG 3 cut(s) 459, 702, 841
BisI GCNGC 3 cut(s) 408, 459, 462
BlsI GCNGC 3 cut(s) 409, 460, 463
Bme18I GGWCC 1 cut(s) 641
BmeT110I CYCGRG 2 cut(s) 230, 858
BmgT120I GGNCC 1 cut(s) 641
BmsI GCATC 1 cut(s) 678
BpiI GAAGAC 1 cut(s) 215
BpuEI CTTGAG 2 cut(s) 77, 713
BsaJI CCNNGG 3 cut(s) 229, 408, 857
BsaXI ACNNNNNCTCC 2 cut(s) 384, 414
Bsc4I CCNNNNNNNGG 2 cut(s) 409, 580
Bse1I ACTGG 1 cut(s) 878
Bse3DI GCAATG 2 cut(s) 266, 526
BseDI CCNNGG 3 cut(s) 229, 408, 857
BseGI GGATG 2 cut(s) 428, 547
BseLI CCNNNNNNNGG 2 cut(s) 409, 580
BseMI GCAATG 2 cut(s) 266, 526
BseNI ACTGG 1 cut(s) 878
BseXI GCAGC 2 cut(s) 445, 473
BsgI GTGCAG 1 cut(s) 639
Bsh1236I CGCG 1 cut(s) 410
BsiHKCI CYCGRG 2 cut(s) 230, 858
BsiSI CCGG 1 cut(s) 779
BslI CCNNNNNNNGG 2 cut(s) 409, 580
BsoBI CYCGRG 2 cut(s) 230, 858
Bsp143I GATC 4 cut(s) 271, 466, 645, 749
BspACI CCGC 4 cut(s) 408, 410, 464, 770
BspFNI CGCG 1 cut(s) 410
BspMAI CTGCAG 2 cut(s) 463, 706
BspPI GGATC 2 cut(s) 279, 474
BspQI GCTCTTC 2 cut(s) 93, 858
BsrDI GCAATG 2 cut(s) 266, 526
BsrI ACTGG 1 cut(s) 878
BssECI CCNNGG 3 cut(s) 229, 408, 857
BssMI GATC 4 cut(s) 271, 466, 645, 749
Bst4CI ACNGT 4 cut(s) 52, 352, 370, 845
Bst6I CTCTTC 2 cut(s) 93, 858
BstC8I GCNNGC 1 cut(s) 382
BstDEI CTNAG 1 cut(s) 28
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 2 cut(s) 428, 547
BstFNI CGCG 1 cut(s) 410
BstKTI GATC 4 cut(s) 274, 469, 648, 752
BstMBI GATC 4 cut(s) 271, 466, 645, 749
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstSFI CTRYAG 3 cut(s) 459, 702, 841
BstUI CGCG 1 cut(s) 410
BstV1I GCAGC 2 cut(s) 445, 473
BstV2I GAAGAC 1 cut(s) 215
BtgI CCRYGG 1 cut(s) 408
BtsCI GGATG 2 cut(s) 428, 547
BtsI GCAGTG 2 cut(s) 36, 81
BtsIMutI CAGTG 5 cut(s) 36, 48, 81, 108, 682
Cac8I GCNNGC 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 641
Cfr42I CCGCGG 1 cut(s) 411
Csp6I GTAC 1 cut(s) 126
CviAII CATG 1 cut(s) 174
CviJI RGCY 9 cut(s) 32, 335, 407, 458, 628, 739, 759, 782, 868
CviKI_1 RGCY 9 cut(s) 32, 335, 407, 458, 628, 739, 759, 782, 868
CviQI GTAC 1 cut(s) 126
DdeI CTNAG 1 cut(s) 28
DpnI GATC 4 cut(s) 273, 468, 647, 751
DpnII GATC 4 cut(s) 271, 466, 645, 749
Eam1104I CTCTTC 2 cut(s) 93, 858
EarI CTCTTC 2 cut(s) 93, 858
Eco47I GGWCC 1 cut(s) 641
Eco88I CYCGRG 2 cut(s) 230, 858
FaeI CATG 1 cut(s) 177
FatI CATG 1 cut(s) 173
Fnu4HI GCNGC 3 cut(s) 408, 459, 462
FokI GGATG 2 cut(s) 435, 534
Fsp4HI GCNGC 3 cut(s) 408, 459, 462
FspBI CTAG 1 cut(s) 756
GluI GCNGC 3 cut(s) 408, 459, 462
HapII CCGG 1 cut(s) 779
Hin1II CATG 1 cut(s) 177
HinfI GANTC 3 cut(s) 17, 698, 766
HpaII CCGG 1 cut(s) 779
HphI GGTGA 3 cut(s) 486, 575, 643
Hpy166II GTNNAC 3 cut(s) 359, 494, 727
Hpy188I TCNGA 3 cut(s) 153, 527, 765
Hpy188III TCNNGA 2 cut(s) 322, 860
Hpy8I GTNNAC 3 cut(s) 359, 494, 727
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 4 cut(s) 52, 352, 370, 845
HpyCH4IV ACGT 2 cut(s) 656, 723
HpyCH4V TGCA 6 cut(s) 259, 380, 461, 519, 620, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 1 cut(s) 28
HpySE526I ACGT 2 cut(s) 656, 723
Hsp92II CATG 1 cut(s) 177
KspI CCGCGG 1 cut(s) 411
Kzo9I GATC 4 cut(s) 271, 466, 645, 749
LguI GCTCTTC 2 cut(s) 93, 858
Lsp1109I GCAGC 2 cut(s) 445, 473
LweI GCATC 1 cut(s) 678
MaeI CTAG 1 cut(s) 756
MaeII ACGT 2 cut(s) 656, 723
MaeIII GTNAC 3 cut(s) 46, 106, 364
MalI GATC 4 cut(s) 273, 468, 647, 751
MboI GATC 4 cut(s) 271, 466, 645, 749
MboII GAAGA 5 cut(s) 64, 80, 215, 845, 875
MluCI AATT 3 cut(s) 22, 130, 417
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 1 cut(s) 505
MnlI CCTC 9 cut(s) 239, 243, 262, 500, 688, 771, 786, 846, 867
MslI CAYNNNNRTG 1 cut(s) 686
MspA1I CMGCKG 2 cut(s) 410, 464
MspI CCGG 1 cut(s) 779
MvnI CGCG 1 cut(s) 410
MwoI GCNNNNNNNGC 1 cut(s) 38
NdeII GATC 4 cut(s) 271, 466, 645, 749
NlaIII CATG 1 cut(s) 177
NmuCI GTSAC 2 cut(s) 46, 106
PciSI GCTCTTC 2 cut(s) 93, 858
PfeI GAWTC 2 cut(s) 17, 766
PflFI GACNNNGTC 1 cut(s) 104
PkrI GCNGC 3 cut(s) 409, 460, 463
PleI GAGTC 1 cut(s) 692
PpsI GAGTC 1 cut(s) 692
PspPI GGNCC 1 cut(s) 641
PstI CTGCAG 2 cut(s) 463, 706
PsyI GACNNNGTC 1 cut(s) 104
RsaI GTAC 1 cut(s) 127
RsaNI GTAC 1 cut(s) 126
RseI CAYNNNNRTG 1 cut(s) 686
SacII CCGCGG 1 cut(s) 411
SapI GCTCTTC 2 cut(s) 93, 858
SatI GCNGC 3 cut(s) 408, 459, 462
Sau3AI GATC 4 cut(s) 271, 466, 645, 749
Sau96I GGNCC 1 cut(s) 641
SchI GAGTC 1 cut(s) 692
SetI ASST 9 cut(s) 34, 192, 364, 460, 493, 619, 659, 726, 778
SfaNI GCATC 1 cut(s) 678
SfcI CTRYAG 3 cut(s) 459, 702, 841
Sfr303I CCGCGG 1 cut(s) 411
SgrBI CCGCGG 1 cut(s) 411
SinI GGWCC 1 cut(s) 641
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 2 cut(s) 92, 692
SmoI CTYRAG 2 cut(s) 92, 692
Sse9I AATT 3 cut(s) 22, 130, 417
SsiI CCGC 4 cut(s) 408, 410, 464, 770
SspMI CTAG 1 cut(s) 756
TaaI ACNGT 4 cut(s) 52, 352, 370, 845
TaiI ACGT 2 cut(s) 659, 726
TaqI TCGA 3 cut(s) 99, 752, 815
TaqII GACCGA 1 cut(s) 658
TasI AATT 3 cut(s) 22, 130, 417
TauI GCSGC 1 cut(s) 410
TfiI GAWTC 2 cut(s) 17, 766
TscAI CASTG 5 cut(s) 43, 55, 88, 115, 682
TseFI GTSAC 2 cut(s) 46, 106
TseI GCWGC 2 cut(s) 458, 461
Tsp45I GTSAC 2 cut(s) 46, 106
TspDTI ATGAA 3 cut(s) 64, 300, 661
TspRI CASTG 5 cut(s) 43, 55, 88, 115, 682
Tth111I GACNNNGTC 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 641
XspI CTAG 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.