Rh7DG017200

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0005619
Physical Location & Seq
Forward (+)
1404715 .. 1408345
3631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG017200.1

Sequence Viewer

Length: 909 bp
ATGGGAAAAGTTTTGGACTCCCATTTCTTGGCTCTCACTGCCATTGTCACTGTGGCATATCAGTCGCTGTTCTTCGTAATCACTGCGCTTCTCAAGTTCGATAAAGTCACTGACTTTGCCGGAGGTACCAATTTTGTTATACTTGCAATACTCACTCTGGTTGTCAAGGGCTCATGGCATTTCAGACAGATAGTGTTGAGTTTGCTTGTGGTAATATGGGGTCTTCGTCTGGGACTGTTTCTCCTGATGAGAATTTTGCAATGGGGGGAGGATCGACGTTTTGATGAAATGCGAGCTAATCTTGGAAAACTGGCAGTATTCTGGATATTTCAGGCTGTCTGGGTATGGACTGTGAGTTTACCTGTAACAGTGGTTAATTCAAGCAACAAAACACCATCTCTTCAAGCTGCGGACGTTATTGGATGGATTATGTGGTCTATTGGTTTTTTGGTTGAAGCTACAGCTGATCAGCAAAAGCTGGTGTTCAAAAATTCTCCTCAAAATAGGGGAAAGTGGTGCAATGTTGGACTATGGAAATACACTAGGCATCCAAATTATTTTGGTGAGATATTCCTTTGGTGGGGAGTATTTGTGGCTTCCACACCTGTATTGGATGGTGCAGAGTGGCTGGTAATTCTTGGACCGATCTTTCTCACGTTGTTGCTTCTTTTCCTCAGTGGCATACCATTGCTAGAGGAATCTGCAGATAAGAAGTATGGAAATGTGGACGGATATAGGCTCTATAAAAGATCAACCAGGTTAATATTAGCTTCTCTTTGGTACATGTTTCTCTTGAGCATGAATTGTAGTAACCGTGACCAACATTTTCTGTTTCAGCCCTCTAATTCCGTTACCCCCGGTCCTGTACAAGAACTTGCCCTCGTGGTTCAAAGCAACTTTTCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1295

Protein Analysis

302

Amino Acids

34.35

Weight (kDa)

9.34

Isoelectric Point (pI)

27.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1295 PF06966 24 - 252 1.6e-91 Protein of unknown function (DUF1295)
Steroid_dh PF02544 135 - 201 5.5e-06 3-oxo-5-alpha-steroid 4-dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 125
AccB1I GGYRCC 1 cut(s) 125
AccB7I CCANNNNNTGG 1 cut(s) 28
AciI CCGC 1 cut(s) 410
AclWI GGATC 1 cut(s) 279
AcsI RAATTY 2 cut(s) 252, 490
AfaI GTAC 3 cut(s) 127, 782, 867
AfiI CCNNNNNNNGG 2 cut(s) 28, 580
AflIII ACRYGT 1 cut(s) 783
AgsI TTSAA 5 cut(s) 381, 404, 455, 487, 890
AjnI CCWGG 1 cut(s) 755
AluBI AGCT 6 cut(s) 296, 407, 458, 464, 478, 770
AluI AGCT 6 cut(s) 296, 407, 458, 464, 478, 770
AlwI GGATC 1 cut(s) 279
AlwNI CAGNNNCTG 1 cut(s) 67
ApeKI GCWGC 1 cut(s) 407
ApoI RAATTY 2 cut(s) 252, 490
Asp718I GGTACC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 88
AspS9I GGNCC 2 cut(s) 641, 860
AsuC2I CCSGG 1 cut(s) 858
AsuHPI GGTGA 1 cut(s) 575
AvaII GGWCC 2 cut(s) 641, 860
BanI GGYRCC 1 cut(s) 125
BanII GRGCYC 1 cut(s) 173
BauI CACGAG 1 cut(s) 881
BbsI GAAGAC 1 cut(s) 215
BbvI GCAGC 1 cut(s) 394
BccI CCATC 3 cut(s) 403, 417, 608
BcgI CGANNNNNNTGC 2 cut(s) 45, 79
BciT130I CCWGG 1 cut(s) 757
BclI TGATCA 1 cut(s) 466
BcnI CCSGG 1 cut(s) 858
BfaI CTAG 2 cut(s) 543, 692
BfmI CTRYAG 2 cut(s) 459, 702
BisI GCNGC 1 cut(s) 408
BlsI GCNGC 1 cut(s) 409
Bme1390I CCNGG 2 cut(s) 757, 858
Bme18I GGWCC 2 cut(s) 641, 860
BmgT120I GGNCC 2 cut(s) 641, 860
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 2 cut(s) 757, 858
BmsI GCATC 1 cut(s) 556
BpiI GAAGAC 1 cut(s) 215
BpuEI CTTGAG 2 cut(s) 77, 814
BpuMI CCSGG 1 cut(s) 858
BsaJI CCNNGG 1 cut(s) 856
BsaXI ACNNNNNCTCC 4 cut(s) 225, 255, 576, 606
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 580
Bse1I ACTGG 1 cut(s) 315
Bse3DI GCAATG 3 cut(s) 266, 526, 686
BseBI CCWGG 1 cut(s) 757
BseDI CCNNGG 1 cut(s) 856
BseGI GGATG 3 cut(s) 428, 547, 619
BseLI CCNNNNNNNGG 2 cut(s) 28, 580
BseMI GCAATG 3 cut(s) 266, 526, 686
BseMII CTCAG 1 cut(s) 688
BseNI ACTGG 1 cut(s) 315
BseRI GAGGAG 1 cut(s) 486
BseXI GCAGC 1 cut(s) 394
BsgI GTGCAG 1 cut(s) 639
BshNI GGYRCC 1 cut(s) 125
BsiSI CCGG 2 cut(s) 120, 858
BslFI GGGAC 1 cut(s) 246
BslI CCNNNNNNNGG 2 cut(s) 28, 580
BsmFI GGGAC 1 cut(s) 246
Bsp1286I GDGCHC 1 cut(s) 173
Bsp1407I TGTACA 1 cut(s) 865
Bsp143I GATC 4 cut(s) 271, 466, 645, 749
BspACI CCGC 1 cut(s) 410
BspCNI CTCAG 1 cut(s) 687
BspLI GGNNCC 1 cut(s) 127
BspMAI CTGCAG 1 cut(s) 706
BspPI GGATC 1 cut(s) 279
BspT107I GGYRCC 1 cut(s) 125
BsrDI GCAATG 3 cut(s) 266, 526, 686
BsrGI TGTACA 1 cut(s) 865
BsrI ACTGG 1 cut(s) 315
BssECI CCNNGG 1 cut(s) 856
BssMI GATC 4 cut(s) 271, 466, 645, 749
BssSI CACGAG 1 cut(s) 881
Bst2BI CACGAG 1 cut(s) 881
Bst2UI CCWGG 1 cut(s) 757
Bst4CI ACNGT 5 cut(s) 52, 237, 352, 370, 815
Bst6I CTCTTC 1 cut(s) 405
BstAUI TGTACA 1 cut(s) 865
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 1 cut(s) 674
BstF5I GGATG 3 cut(s) 428, 547, 619
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 4 cut(s) 274, 469, 648, 752
BstMBI GATC 4 cut(s) 271, 466, 645, 749
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstNI CCWGG 1 cut(s) 757
BstNSI RCATGY 1 cut(s) 787
BstSCI CCNGG 2 cut(s) 755, 856
BstSFI CTRYAG 2 cut(s) 459, 702
BstV1I GCAGC 1 cut(s) 394
BstV2I GAAGAC 1 cut(s) 215
BtsCI GGATG 3 cut(s) 428, 547, 619
BtsI GCAGTG 2 cut(s) 36, 81
BtsIMutI CAGTG 6 cut(s) 36, 48, 81, 108, 375, 682
Cac8I GCNNGC 1 cut(s) 294
CaiI CAGNNNCTG 1 cut(s) 67
CfoI GCGC 1 cut(s) 88
Cfr13I GGNCC 2 cut(s) 641, 860
CsiI ACCWGGT 1 cut(s) 755
Csp6I GTAC 3 cut(s) 126, 781, 866
CviAII CATG 3 cut(s) 174, 784, 799
CviQI GTAC 3 cut(s) 126, 781, 866
DdeI CTNAG 1 cut(s) 674
DpnI GATC 4 cut(s) 273, 468, 647, 751
DpnII GATC 4 cut(s) 271, 466, 645, 749
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
Eco24I GRGCYC 1 cut(s) 173
Eco47I GGWCC 2 cut(s) 641, 860
EcoRII CCWGG 1 cut(s) 755
EcoT38I GRGCYC 1 cut(s) 173
FaeI CATG 3 cut(s) 177, 787, 802
FaqI GGGAC 1 cut(s) 246
FatI CATG 3 cut(s) 173, 783, 798
FbaI TGATCA 1 cut(s) 466
Fnu4HI GCNGC 1 cut(s) 408
FokI GGATG 3 cut(s) 435, 534, 626
FriOI GRGCYC 1 cut(s) 173
Fsp4HI GCNGC 1 cut(s) 408
FspBI CTAG 2 cut(s) 543, 692
GlaI GCGC 1 cut(s) 87
GluI GCNGC 1 cut(s) 408
HapII CCGG 2 cut(s) 120, 858
HhaI GCGC 1 cut(s) 88
Hin1II CATG 3 cut(s) 177, 787, 802
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HinfI GANTC 2 cut(s) 17, 698
HpaII CCGG 2 cut(s) 120, 858
HphI GGTGA 1 cut(s) 575
Hpy166II GTNNAC 2 cut(s) 359, 727
Hpy188I TCNGA 1 cut(s) 185
Hpy188III TCNNGA 3 cut(s) 244, 322, 793
Hpy8I GTNNAC 2 cut(s) 359, 727
Hpy99I CGWCG 1 cut(s) 279
HpyCH4III ACNGT 5 cut(s) 52, 237, 352, 370, 815
HpyCH4IV ACGT 3 cut(s) 277, 414, 656
HpyCH4V TGCA 5 cut(s) 146, 259, 519, 620, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 1 cut(s) 674
HpySE526I ACGT 3 cut(s) 277, 414, 656
Hsp92II CATG 3 cut(s) 177, 787, 802
HspAI GCGC 1 cut(s) 86
KpnI GGTACC 1 cut(s) 129
Ksp22I TGATCA 1 cut(s) 466
Kzo9I GATC 4 cut(s) 271, 466, 645, 749
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 1 cut(s) 556
MabI ACCWGGT 1 cut(s) 755
MaeI CTAG 2 cut(s) 543, 692
MaeII ACGT 3 cut(s) 277, 414, 656
MaeIII GTNAC 6 cut(s) 46, 106, 364, 809, 815, 850
MalI GATC 4 cut(s) 273, 468, 647, 751
MboI GATC 4 cut(s) 271, 466, 645, 749
MboII GAAGA 3 cut(s) 64, 215, 392
MhlI GDGCHC 1 cut(s) 173
MluCI AATT 8 cut(s) 130, 252, 376, 490, 553, 633, 802, 844
MlyI GAGTC 1 cut(s) 11
MmeI TCCRAC 1 cut(s) 505
MnlI CCTC 7 cut(s) 116, 262, 507, 683, 688, 850, 890
MseI TTAA 2 cut(s) 375, 761
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 2 cut(s) 120, 858
MspR9I CCNGG 2 cut(s) 757, 858
MvaI CCWGG 1 cut(s) 757
MwoI GCNNNNNNNGC 1 cut(s) 38
NciI CCSGG 1 cut(s) 858
NdeII GATC 4 cut(s) 271, 466, 645, 749
NlaIII CATG 3 cut(s) 177, 787, 802
NlaIV GGNNCC 1 cut(s) 127
NmuCI GTSAC 3 cut(s) 46, 106, 815
NspI RCATGY 1 cut(s) 787
PciI ACATGT 1 cut(s) 783
PfeI GAWTC 1 cut(s) 698
PflMI CCANNNNNTGG 1 cut(s) 28
PkrI GCNGC 1 cut(s) 409
PleI GAGTC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 11
PscI ACATGT 1 cut(s) 783
Psp6I CCWGG 1 cut(s) 755
PspGI CCWGG 1 cut(s) 755
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 2 cut(s) 641, 860
PstI CTGCAG 1 cut(s) 706
PstNI CAGNNNCTG 1 cut(s) 67
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 3 cut(s) 127, 782, 867
RsaNI GTAC 3 cut(s) 126, 781, 866
SaqAI TTAA 2 cut(s) 375, 761
SatI GCNGC 1 cut(s) 408
Sau3AI GATC 4 cut(s) 271, 466, 645, 749
Sau96I GGNCC 2 cut(s) 641, 860
SchI GAGTC 1 cut(s) 11
ScrFI CCNGG 2 cut(s) 757, 858
SduI GDGCHC 1 cut(s) 173
SexAI ACCWGGT 1 cut(s) 755
SfaNI GCATC 1 cut(s) 556
SfcI CTRYAG 2 cut(s) 459, 702
SinI GGWCC 2 cut(s) 641, 860
SmlI CTYRAG 2 cut(s) 92, 793
SmoI CTYRAG 2 cut(s) 92, 793
Sse9I AATT 8 cut(s) 130, 252, 376, 490, 553, 633, 802, 844
SsiI CCGC 1 cut(s) 410
SspI AATATT 1 cut(s) 765
SspMI CTAG 2 cut(s) 543, 692
StyD4I CCNGG 2 cut(s) 755, 856
TaaI ACNGT 5 cut(s) 52, 237, 352, 370, 815
TaiI ACGT 3 cut(s) 280, 417, 659
TaqI TCGA 2 cut(s) 99, 274
TaqII GACCGA 1 cut(s) 658
TasI AATT 8 cut(s) 130, 252, 376, 490, 553, 633, 802, 844
TatI WGTACW 1 cut(s) 865
TfiI GAWTC 1 cut(s) 698
Tru1I TTAA 2 cut(s) 375, 761
Tru9I TTAA 2 cut(s) 375, 761
TscAI CASTG 6 cut(s) 43, 55, 88, 115, 375, 682
TseFI GTSAC 3 cut(s) 46, 106, 815
TseI GCWGC 1 cut(s) 407
Tsp45I GTSAC 3 cut(s) 46, 106, 815
TspDTI ATGAA 2 cut(s) 300, 815
TspGWI ACGGA 2 cut(s) 744, 838
TspRI CASTG 6 cut(s) 43, 55, 88, 115, 375, 682
Van91I CCANNNNNTGG 1 cut(s) 28
VpaK11BI GGWCC 2 cut(s) 641, 860
XapI RAATTY 2 cut(s) 252, 490
XceI RCATGY 1 cut(s) 787
XcmI CCANNNNNNNNNTGG 2 cut(s) 49, 607
XspI CTAG 2 cut(s) 543, 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.