RchiOBHm_Chr7g0178611

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0005619
Physical Location & Seq
Forward (+)
1228585 .. 1231470
2886 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15917

Sequence Viewer

Length: 924 bp
ATGGGAAAAGTTTTGGACTCCCATTTCTTGGCTCTCACTGCCATTGTCACTGTGGCGTATCAGTCGTTGTTCTTCGTAATCACTGCGCTTCTCAAGTTTGATAAAGTCACTGACTTTGCCGGAAGTACCAATTTTGTTATACTTGCAATACTCACTCTGGTTGTCAAGGGCTCATGGCATTTCAGACAGATAGTGTTGAGTTTGCTTGTGGTAATATGGGGTCTTCGTCTGGGACTGTTTCTCCTGATGAGAATTTTGCAATGGGGGGAGGATCGACGTTTTGATGAAATGCGAGCTAATCTTGGAAAACTGGCAGTATTCTGGATATTTCAGGCTGTCTGGGTATGGACTGTGAGTTTACCTGTAACAGTGGTTAATTCAAGCAACAAAACACCATCTCTTCAAGCTGCGGACGTTATTGGATGGATTATGTGGTCTATTGGTTTTTTGGTTGAAGCTACAGCTGATCAGCAAAAGCTGGTGTTCAAAAACTCTCCTCAAAATAGGGGAAAGTGGTGCAATGTTGGACTATGGAAATACACTAGGCATCCAAATTATTTTGGTGAGATATTCCTTTGGTGGGGAGTATTTGTGGCTTCCACACCTGTATTGGATGGTGCAGAGTGGCTGGTAATTCTTGGACCGATCTTTCTCACGTTGTTACTTCTTTTCCTCAGTGGCATACCATTGCTAGAGGAATCTGCAGATAAGAAGTATGGAAATATGGACGGATATAGGCTCTATAAAAGATCAACCAGCCCTCTAATTCCGCTACCCCCGGTCCTGTACAAGAACTTGCCCTCGTGGTTCAAAGGAACTTTTCTCTTTGAATTTCCCCTGTATAGTCGTAATCTTCCTCAAGAACCACCGAACTGGTGTAGAACAAGCCCGATAGAAAGAAGCGACGAATTGAAGATTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1295

Protein Analysis

307

Amino Acids

35.12

Weight (kDa)

9.37

Isoelectric Point (pI)

34.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1295 PF06966 24 - 252 1.8e-91 Protein of unknown function (DUF1295)
Steroid_dh PF02544 135 - 201 5.7e-06 3-oxo-5-alpha-steroid 4-dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 28
AciI CCGC 2 cut(s) 410, 770
AclWI GGATC 1 cut(s) 279
AcsI RAATTY 2 cut(s) 252, 830
AfaI GTAC 2 cut(s) 127, 788
AfiI CCNNNNNNNGG 2 cut(s) 28, 580
AgsI TTSAA 7 cut(s) 381, 404, 455, 487, 811, 830, 913
AjuI GAANNNNNNNTTGG 1 cut(s) 900
AluBI AGCT 5 cut(s) 296, 407, 458, 464, 478
AluI AGCT 5 cut(s) 296, 407, 458, 464, 478
AlwI GGATC 1 cut(s) 279
ApeKI GCWGC 1 cut(s) 407
ApoI RAATTY 2 cut(s) 252, 830
AspLEI GCGC 1 cut(s) 88
AspS9I GGNCC 2 cut(s) 641, 781
AsuC2I CCSGG 1 cut(s) 779
AsuHPI GGTGA 1 cut(s) 575
AvaII GGWCC 2 cut(s) 641, 781
BanII GRGCYC 1 cut(s) 173
BauI CACGAG 1 cut(s) 802
BbsI GAAGAC 1 cut(s) 215
BbvI GCAGC 1 cut(s) 394
BccI CCATC 3 cut(s) 403, 417, 608
BclI TGATCA 1 cut(s) 466
BcnI CCSGG 1 cut(s) 779
BfaI CTAG 2 cut(s) 543, 692
BfmI CTRYAG 2 cut(s) 459, 702
BisI GCNGC 1 cut(s) 408
BlsI GCNGC 1 cut(s) 409
Bme1390I CCNGG 1 cut(s) 779
Bme18I GGWCC 2 cut(s) 641, 781
BmgT120I GGNCC 2 cut(s) 641, 781
BmrFI CCNGG 1 cut(s) 779
BmsI GCATC 1 cut(s) 556
BpiI GAAGAC 1 cut(s) 215
BpuEI CTTGAG 2 cut(s) 77, 843
BpuMI CCSGG 1 cut(s) 779
BsaJI CCNNGG 1 cut(s) 777
BsaXI ACNNNNNCTCC 4 cut(s) 225, 255, 576, 606
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 580
Bse1I ACTGG 2 cut(s) 315, 878
Bse3DI GCAATG 3 cut(s) 266, 526, 686
BseDI CCNNGG 1 cut(s) 777
BseGI GGATG 3 cut(s) 428, 547, 619
BseLI CCNNNNNNNGG 2 cut(s) 28, 580
BseMI GCAATG 3 cut(s) 266, 526, 686
BseMII CTCAG 1 cut(s) 688
BseNI ACTGG 2 cut(s) 315, 878
BseRI GAGGAG 1 cut(s) 486
BseXI GCAGC 1 cut(s) 394
BsgI GTGCAG 1 cut(s) 639
BsiSI CCGG 2 cut(s) 120, 779
BslFI GGGAC 1 cut(s) 246
BslI CCNNNNNNNGG 2 cut(s) 28, 580
BsmFI GGGAC 1 cut(s) 246
Bsp1286I GDGCHC 1 cut(s) 173
Bsp1407I TGTACA 1 cut(s) 786
Bsp143I GATC 4 cut(s) 271, 466, 645, 749
BspACI CCGC 2 cut(s) 410, 770
BspCNI CTCAG 1 cut(s) 687
BspMAI CTGCAG 1 cut(s) 706
BspPI GGATC 1 cut(s) 279
BsrDI GCAATG 3 cut(s) 266, 526, 686
BsrGI TGTACA 1 cut(s) 786
BsrI ACTGG 2 cut(s) 315, 878
BssECI CCNNGG 1 cut(s) 777
BssMI GATC 4 cut(s) 271, 466, 645, 749
BssSI CACGAG 1 cut(s) 802
Bst2BI CACGAG 1 cut(s) 802
Bst4CI ACNGT 4 cut(s) 52, 237, 352, 370
Bst6I CTCTTC 1 cut(s) 405
BstAUI TGTACA 1 cut(s) 786
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 1 cut(s) 674
BstF5I GGATG 3 cut(s) 428, 547, 619
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 4 cut(s) 274, 469, 648, 752
BstMBI GATC 4 cut(s) 271, 466, 645, 749
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 777
BstSFI CTRYAG 2 cut(s) 459, 702
BstV1I GCAGC 1 cut(s) 394
BstV2I GAAGAC 1 cut(s) 215
BstXI CCANNNNNNTGG 1 cut(s) 873
BtsCI GGATG 3 cut(s) 428, 547, 619
BtsI GCAGTG 2 cut(s) 36, 81
BtsIMutI CAGTG 6 cut(s) 36, 48, 81, 108, 375, 682
Cac8I GCNNGC 1 cut(s) 294
CfoI GCGC 1 cut(s) 88
Cfr13I GGNCC 2 cut(s) 641, 781
Csp6I GTAC 2 cut(s) 126, 787
CviAII CATG 1 cut(s) 174
CviQI GTAC 2 cut(s) 126, 787
DdeI CTNAG 1 cut(s) 674
DpnI GATC 4 cut(s) 273, 468, 647, 751
DpnII GATC 4 cut(s) 271, 466, 645, 749
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
Eco24I GRGCYC 1 cut(s) 173
Eco47I GGWCC 2 cut(s) 641, 781
EcoT38I GRGCYC 1 cut(s) 173
FaeI CATG 1 cut(s) 177
FaqI GGGAC 1 cut(s) 246
FatI CATG 1 cut(s) 173
FbaI TGATCA 1 cut(s) 466
Fnu4HI GCNGC 1 cut(s) 408
FokI GGATG 3 cut(s) 435, 534, 626
FriOI GRGCYC 1 cut(s) 173
Fsp4HI GCNGC 1 cut(s) 408
FspBI CTAG 2 cut(s) 543, 692
GlaI GCGC 1 cut(s) 87
GluI GCNGC 1 cut(s) 408
HapII CCGG 2 cut(s) 120, 779
HhaI GCGC 1 cut(s) 88
Hin1II CATG 1 cut(s) 177
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HinfI GANTC 2 cut(s) 17, 698
HpaII CCGG 2 cut(s) 120, 779
HphI GGTGA 1 cut(s) 575
Hpy166II GTNNAC 1 cut(s) 359
Hpy188I TCNGA 1 cut(s) 185
Hpy188III TCNNGA 3 cut(s) 244, 322, 860
Hpy8I GTNNAC 1 cut(s) 359
Hpy99I CGWCG 2 cut(s) 279, 908
HpyCH4III ACNGT 4 cut(s) 52, 237, 352, 370
HpyCH4IV ACGT 3 cut(s) 277, 414, 656
HpyCH4V TGCA 5 cut(s) 146, 259, 519, 620, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 1 cut(s) 674
HpySE526I ACGT 3 cut(s) 277, 414, 656
Hsp92II CATG 1 cut(s) 177
HspAI GCGC 1 cut(s) 86
Ksp22I TGATCA 1 cut(s) 466
Kzo9I GATC 4 cut(s) 271, 466, 645, 749
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 1 cut(s) 556
MaeI CTAG 2 cut(s) 543, 692
MaeII ACGT 3 cut(s) 277, 414, 656
MaeIII GTNAC 4 cut(s) 46, 106, 364, 660
MalI GATC 4 cut(s) 273, 468, 647, 751
MboI GATC 4 cut(s) 271, 466, 645, 749
MboII GAAGA 4 cut(s) 64, 215, 392, 845
MhlI GDGCHC 1 cut(s) 173
MluCI AATT 8 cut(s) 130, 252, 376, 553, 633, 765, 830, 908
MlyI GAGTC 1 cut(s) 11
MmeI TCCRAC 1 cut(s) 505
MnlI CCTC 7 cut(s) 262, 507, 683, 688, 771, 811, 867
MseI TTAA 1 cut(s) 375
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 2 cut(s) 120, 779
MspR9I CCNGG 1 cut(s) 779
MwoI GCNNNNNNNGC 1 cut(s) 38
NciI CCSGG 1 cut(s) 779
NdeII GATC 4 cut(s) 271, 466, 645, 749
NlaIII CATG 1 cut(s) 177
NmuCI GTSAC 2 cut(s) 46, 106
PfeI GAWTC 1 cut(s) 698
PflMI CCANNNNNTGG 1 cut(s) 28
PkrI GCNGC 1 cut(s) 409
PleI GAGTC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 11
PspPI GGNCC 2 cut(s) 641, 781
PstI CTGCAG 1 cut(s) 706
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 2 cut(s) 127, 788
RsaNI GTAC 2 cut(s) 126, 787
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 1 cut(s) 408
Sau3AI GATC 4 cut(s) 271, 466, 645, 749
Sau96I GGNCC 2 cut(s) 641, 781
SchI GAGTC 1 cut(s) 11
ScrFI CCNGG 1 cut(s) 779
SduI GDGCHC 1 cut(s) 173
SfaNI GCATC 1 cut(s) 556
SfcI CTRYAG 2 cut(s) 459, 702
SinI GGWCC 2 cut(s) 641, 781
SmlI CTYRAG 2 cut(s) 92, 858
SmoI CTYRAG 2 cut(s) 92, 858
Sse9I AATT 8 cut(s) 130, 252, 376, 553, 633, 765, 830, 908
SsiI CCGC 2 cut(s) 410, 770
SspMI CTAG 2 cut(s) 543, 692
StyD4I CCNGG 1 cut(s) 777
TaaI ACNGT 4 cut(s) 52, 237, 352, 370
TaiI ACGT 3 cut(s) 280, 417, 659
TaqI TCGA 1 cut(s) 274
TaqII GACCGA 1 cut(s) 658
TasI AATT 8 cut(s) 130, 252, 376, 553, 633, 765, 830, 908
TatI WGTACW 1 cut(s) 786
TfiI GAWTC 1 cut(s) 698
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 6 cut(s) 43, 55, 88, 115, 375, 682
TseFI GTSAC 2 cut(s) 46, 106
TseI GCWGC 1 cut(s) 407
Tsp45I GTSAC 2 cut(s) 46, 106
TspDTI ATGAA 1 cut(s) 300
TspGWI ACGGA 1 cut(s) 744
TspRI CASTG 6 cut(s) 43, 55, 88, 115, 375, 682
Van91I CCANNNNNTGG 1 cut(s) 28
VpaK11BI GGWCC 2 cut(s) 641, 781
XapI RAATTY 2 cut(s) 252, 830
XcmI CCANNNNNNNNNTGG 2 cut(s) 49, 607
XspI CTAG 2 cut(s) 543, 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.