Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009946

Physical Location & Seq

Genomic Coordinates Forward (+)

7614491 .. 7618007

Gene Structure 3517 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1109200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 849 bp

ATGTTCTCAGGCTCCCAATTCGATGCCACGAACGCCTTCTCCGGCGGCGGTTTCATGGCTTCTCAGTCCACTCAATTCGGCGATTCCACTCCCTCTCCGGCCAAGAGACGCGAAACTCACGGATTGGTTCCGGTGACGGTGAAGCAGGTTAGCGAAGCCTATCAGTCCGGTGACGAAAAATCGAACTTTGTGATCAGCGGGGCAGATGTTGCTAATGTATCGGTGGTTGGGATGGTTTTTGACAAAGCCGAAAGGAACACTGATGTTGATTTCACTGTGGATGACGGAACAGGGCGAATCAAGTGCAGAAGATGGGTGAATGAGAATTTTGACTCCAGAGAAATGCAGGAAATAGAAGATGGAATGTATGTTCGTGTTAATGGACACTTGAAAGTCTTTCAAGGTGTTCGACAGATAGTTGCCTTCTCTGTGAGGCCTGTGAAAAACTTCGATGAAGTTGCTTTCCACTTCATTGAGTGCATACATACCCACCTACTGACATCCAAGTTACAGCTGCAAGGGAATGCTGCCACTCAAGCTCAGCCTATGGACTCATCTCTGAACACTCCTGTGCGGAGTGGGTCAACTGGATATCAGACAGCCCCGTCAAACCAATTCTCCGGGCAAGTTAGCGTTGATGGACTAAAGAGCTGTGATCAGTTGGTTCTTGATTATCTGCAGCAGCCTTCAAGCATTGGAAAAGAAAAGGGGATACACAGAGATGAACTTTCCCAGCACCTGAAAGTTCCTGTGGGAAAGATCTTGGAAGCTATCAGATCTCTTGAGGAGGAAGGCTTAATATATTCCACGATCGATGAGTTTCACTACAAGTCAGCAGCATATGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000278 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005662 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006281 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016458 GO:0018130 GO:0019222 GO:0019438 GO:0022402 GO:0030894 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0032993 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035861 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044786 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0090304 GO:0090734 GO:1901360 GO:1901362 GO:1901576 GO:1902295 GO:1902318 GO:1902969 GO:1902981 GO:1903047

KEGG Pathways

Metabolic & Signaling

ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460

Pfam Domains

Protein Families

RPA_C tRNA_anti-codon

Protein Analysis

283

Amino Acids

31.01

Weight (kDa)

5.5

Isoelectric Point (pI)

48.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_anti-codon	PF01336	73 - 146	8e-10	OB-fold nucleic acid binding domain
RPA_C	PF08784	164 - 273	2.8e-17	Replication protein A C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009946)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24490 AT2G24490
fragaria_vesca	FvH4_2g36940 FvH4_2g36940 FvH4_2g36940
malus_domestica	MD08G1131500.v1.1 MD08G1131600.v1.1 MD15G1109200.v1.1
prunus_persica	Prupe.1G461400_v2.0.a1
pyrus_communis	pycom08g11020
rosa_chinensis	RchiOBHm_Chr6g0304821
rosa_laevigata	RLG00000010946
rosa_multiflora	Rmu_ssc0000426.1_g000002
rosa_roxburghii	Rroxscaffold_7G00163440
rosa_rugosa	Rorug06G0335100 Rorug06G0335200
rosa_samantha	Rh6AG446800 Rh6BG454800 Rh6CG460300 Rh6DG447200
rosa_wichuraiana	Rw6G038880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	604
Acc36I	ACCTGC	1 cut(s)	136
AccII	CGCG	1 cut(s)	111
AciI	CCGC	4 cut(s)	45, 48, 198, 574
AcoI	YGGCCR	1 cut(s)	99
AcsI	RAATTY	1 cut(s)	325
AgsI	TTSAA	3 cut(s)	391, 401, 690
AleI	CACNNNNGTG	1 cut(s)	569
AloI	GAACNNNNNNTCC	4 cut(s)	23, 55, 354, 386
AluBI	AGCT	4 cut(s)	514, 539, 651, 770
AluI	AGCT	4 cut(s)	514, 539, 651, 770
Alw26I	GTCTC	1 cut(s)	100
AlwNI	CAGNNNCTG	1 cut(s)	739
AoxI	GGCC	2 cut(s)	99, 434
ApeKI	GCWGC	5 cut(s)	514, 527, 679, 682, 836
ApoI	RAATTY	1 cut(s)	325
Asp700I	GAANNNNTTC	2 cut(s)	35, 446
AsuC2I	CCSGG	1 cut(s)	622
AsuHPI	GGTGA	4 cut(s)	145, 151, 182, 328
BbvI	GCAGC	4 cut(s)	501, 514, 691, 694
BccI	CCATC	4 cut(s)	226, 306, 353, 632
BcgI	CGANNNNNNTGC	4 cut(s)	285, 319, 440, 474
BciVI	GTATCC	1 cut(s)	705
BclI	TGATCA	2 cut(s)	192, 655
BcnI	CCSGG	1 cut(s)	622
BcoDI	GTCTC	1 cut(s)	100
BfmI	CTRYAG	1 cut(s)	677
BfuAI	ACCTGC	1 cut(s)	136
BfuI	GTATCC	1 cut(s)	705
BglII	AGATCT	2 cut(s)	759, 776
BisI	GCNGC	6 cut(s)	46, 515, 528, 680, 683, 837
BlpI	GCTNAGC	1 cut(s)	540
BlsI	GCNGC	6 cut(s)	47, 516, 529, 681, 684, 838
Bme1390I	CCNGG	1 cut(s)	622
BmiI	GGNNCC	2 cut(s)	13, 129
BmrFI	CCNGG	1 cut(s)	622
BmsI	GCATC	1 cut(s)	13
BpmI	CTGGAG	1 cut(s)	319
Bpu1102I	GCTNAGC	1 cut(s)	540
BpuEI	CTTGAG	2 cut(s)	519, 803
BpuMI	CCSGG	1 cut(s)	622
Bsa29I	ATCGAT	1 cut(s)	813
BsaWI	WCCGGW	2 cut(s)	130, 167
BsaXI	ACNNNNNCTCC	6 cut(s)	23, 53, 79, 109, 602, 632
Bse1I	ACTGG	1 cut(s)	592
BseCI	ATCGAT	1 cut(s)	813
BseGI	GGATG	3 cut(s)	237, 286, 500
BseMII	CTCAG	3 cut(s)	21, 77, 554
BseNI	ACTGG	1 cut(s)	592
BseRI	GAGGAG	1 cut(s)	800
BseXI	GCAGC	4 cut(s)	501, 514, 691, 694
BseYI	CCCAGC	1 cut(s)	732
BsgI	GTGCAG	1 cut(s)	325
Bsh1236I	CGCG	1 cut(s)	111
Bsh1285I	CGRYCG	1 cut(s)	813
BshFI	GGCC	2 cut(s)	101, 436
BshVI	ATCGAT	1 cut(s)	813
BsiEI	CGRYCG	1 cut(s)	813
BsiSI	CCGG	5 cut(s)	42, 98, 131, 168, 621
BsmAI	GTCTC	1 cut(s)	100
BsmBI	CGTCTC	1 cut(s)	100
BsmI	GAATGC	1 cut(s)	529
BsnI	GGCC	2 cut(s)	101, 436
Bsp143I	GATC	5 cut(s)	192, 655, 759, 776, 810
Bsp1720I	GCTNAGC	1 cut(s)	540
BspACI	CCGC	4 cut(s)	45, 48, 198, 574
BspANI	GGCC	2 cut(s)	101, 436
BspCNI	CTCAG	3 cut(s)	20, 76, 553
BspDI	ATCGAT	1 cut(s)	813
BspFNI	CGCG	1 cut(s)	111
BspLI	GGNNCC	2 cut(s)	13, 129
BspMAI	CTGCAG	1 cut(s)	681
BspMI	ACCTGC	1 cut(s)	136
BsrI	ACTGG	1 cut(s)	592
BssMI	GATC	5 cut(s)	192, 655, 759, 776, 810
Bst4CI	ACNGT	2 cut(s)	139, 277
BstAPI	GCANNNNNTGC	1 cut(s)	209
BstDEI	CTNAG	3 cut(s)	7, 63, 540
BstF5I	GGATG	3 cut(s)	237, 286, 500
BstFNI	CGCG	1 cut(s)	111
BstKTI	GATC	5 cut(s)	195, 658, 762, 779, 813
BstMAI	GTCTC	1 cut(s)	100
BstMBI	GATC	5 cut(s)	192, 655, 759, 776, 810
BstMCI	CGRYCG	1 cut(s)	813
BstMWI	GCNNNNNNNGC	3 cut(s)	32, 209, 536
BstSCI	CCNGG	1 cut(s)	620
BstSFI	CTRYAG	1 cut(s)	677
BstUI	CGCG	1 cut(s)	111
BstV1I	GCAGC	4 cut(s)	501, 514, 691, 694
BstX2I	RGATCY	2 cut(s)	759, 776
BstYI	RGATCY	2 cut(s)	759, 776
Bsu15I	ATCGAT	1 cut(s)	813
BsuI	GTATCC	1 cut(s)	705
BsuRI	GGCC	2 cut(s)	101, 436
BsuTUI	ATCGAT	1 cut(s)	813
BtsCI	GGATG	3 cut(s)	237, 286, 500
BtsIMutI	CAGTG	2 cut(s)	258, 273
BveI	ACCTGC	1 cut(s)	136
CaiI	CAGNNNCTG	1 cut(s)	739
ClaI	ATCGAT	1 cut(s)	813
CseI	GACGC	1 cut(s)	117
CviAII	CATG	1 cut(s)	55
DdeI	CTNAG	3 cut(s)	7, 63, 540
DpnI	GATC	5 cut(s)	194, 657, 761, 778, 812
DpnII	GATC	5 cut(s)	192, 655, 759, 776, 810
DrdI	GACNNNNNNGTC	1 cut(s)	604
DseDI	GACNNNNNNGTC	1 cut(s)	604
EaeI	YGGCCR	1 cut(s)	99
Eco147I	AGGCCT	1 cut(s)	436
Eco32I	GATATC	1 cut(s)	593
EcoRV	GATATC	1 cut(s)	593
Esp3I	CGTCTC	1 cut(s)	100
FaeI	CATG	1 cut(s)	58
FaiI	YATR	8 cut(s)	56, 369, 482, 486, 548, 802, 841, 843
FatI	CATG	1 cut(s)	54
FauI	CCCGC	1 cut(s)	191
FauNDI	CATATG	1 cut(s)	841
FbaI	TGATCA	2 cut(s)	192, 655
Fnu4HI	GCNGC	6 cut(s)	46, 515, 528, 680, 683, 837
FokI	GGATG	3 cut(s)	244, 293, 487
Fsp4HI	GCNGC	6 cut(s)	46, 515, 528, 680, 683, 837
GluI	GCNGC	6 cut(s)	46, 515, 528, 680, 683, 837
GsaI	CCCAGC	1 cut(s)	736
GsuI	CTGGAG	1 cut(s)	319
HaeIII	GGCC	2 cut(s)	101, 436
HapII	CCGG	5 cut(s)	42, 98, 131, 168, 621
HgaI	GACGC	1 cut(s)	117
Hin1II	CATG	1 cut(s)	58
HincII	GTYRAC	1 cut(s)	585
HindII	GTYRAC	1 cut(s)	585
HinfI	GANTC	4 cut(s)	83, 297, 332, 551
HpaII	CCGG	5 cut(s)	42, 98, 131, 168, 621
HphI	GGTGA	4 cut(s)	145, 151, 182, 328
Hpy166II	GTNNAC	2 cut(s)	69, 585
Hpy188I	TCNGA	3 cut(s)	561, 597, 776
Hpy188III	TCNNGA	3 cut(s)	336, 668, 782
Hpy8I	GTNNAC	2 cut(s)	69, 585
HpyAV	CCTTC	4 cut(s)	46, 433, 696, 785
HpyCH4III	ACNGT	2 cut(s)	139, 277
HpyCH4V	TGCA	5 cut(s)	306, 346, 480, 517, 679
HpyF10VI	GCNNNNNNNGC	3 cut(s)	32, 209, 536
HpyF3I	CTNAG	3 cut(s)	7, 63, 540
Hsp92II	CATG	1 cut(s)	58
Ksp22I	TGATCA	2 cut(s)	192, 655
Kzo9I	GATC	5 cut(s)	192, 655, 759, 776, 810
LmnI	GCTCC	1 cut(s)	17
Lsp1109I	GCAGC	4 cut(s)	501, 514, 691, 694
LweI	GCATC	1 cut(s)	13
MaeIII	GTNAC	3 cut(s)	133, 170, 507
MalI	GATC	5 cut(s)	194, 657, 761, 778, 812
MboI	GATC	5 cut(s)	192, 655, 759, 776, 810
MboII	GAAGA	2 cut(s)	321, 368
MflI	RGATCY	2 cut(s)	759, 776
MluCI	AATT	4 cut(s)	17, 74, 325, 614
MlyI	GAGTC	2 cut(s)	326, 545
MnlI	CCTC	4 cut(s)	103, 426, 778, 781
MroXI	GAANNNNTTC	2 cut(s)	35, 446
MseI	TTAA	3 cut(s)	378, 797, 847
MslI	CAYNNNNRTG	2 cut(s)	569, 720
MspA1I	CMGCKG	2 cut(s)	198, 514
MspI	CCGG	5 cut(s)	42, 98, 131, 168, 621
MspR9I	CCNGG	1 cut(s)	622
Mva1269I	GAATGC	1 cut(s)	529
MvnI	CGCG	1 cut(s)	111
MwoI	GCNNNNNNNGC	3 cut(s)	32, 209, 536
NciI	CCSGG	1 cut(s)	622
NdeI	CATATG	1 cut(s)	841
NdeII	GATC	5 cut(s)	192, 655, 759, 776, 810
NlaIII	CATG	1 cut(s)	58
NlaIV	GGNNCC	2 cut(s)	13, 129
NmuCI	GTSAC	2 cut(s)	133, 170
OliI	CACNNNNGTG	1 cut(s)	569
PceI	AGGCCT	1 cut(s)	436
PctI	GAATGC	1 cut(s)	529
PdmI	GAANNNNTTC	2 cut(s)	35, 446
PfeI	GAWTC	2 cut(s)	83, 297
PkrI	GCNGC	6 cut(s)	47, 516, 529, 681, 684, 838
Ple19I	CGATCG	1 cut(s)	813
PleI	GAGTC	2 cut(s)	326, 545
PpsI	GAGTC	2 cut(s)	326, 545
PspFI	CCCAGC	1 cut(s)	732
PspN4I	GGNNCC	2 cut(s)	13, 129
PstI	CTGCAG	1 cut(s)	681
PstNI	CAGNNNCTG	1 cut(s)	739
PsuI	RGATCY	2 cut(s)	759, 776
PvuI	CGATCG	1 cut(s)	813
PvuII	CAGCTG	1 cut(s)	514
RseI	CAYNNNNRTG	2 cut(s)	569, 720
SaqAI	TTAA	3 cut(s)	378, 797, 847
SatI	GCNGC	6 cut(s)	46, 515, 528, 680, 683, 837
Sau3AI	GATC	5 cut(s)	192, 655, 759, 776, 810
SchI	GAGTC	2 cut(s)	326, 545
ScrFI	CCNGG	1 cut(s)	622
SetI	ASST	8 cut(s)	150, 406, 495, 516, 541, 653, 741, 772
SfaNI	GCATC	1 cut(s)	13
SfcI	CTRYAG	1 cut(s)	677
SmiMI	CAYNNNNRTG	2 cut(s)	569, 720
SmlI	CTYRAG	2 cut(s)	534, 782
SmoI	CTYRAG	2 cut(s)	534, 782
Sse9I	AATT	4 cut(s)	17, 74, 325, 614
SseBI	AGGCCT	1 cut(s)	436
SsiI	CCGC	4 cut(s)	45, 48, 198, 574
StuI	AGGCCT	1 cut(s)	436
StyD4I	CCNGG	1 cut(s)	620
TaaI	ACNGT	2 cut(s)	139, 277
TaqI	TCGA	5 cut(s)	21, 182, 409, 450, 813
TasI	AATT	4 cut(s)	17, 74, 325, 614
TauI	GCSGC	1 cut(s)	48
TfiI	GAWTC	2 cut(s)	83, 297
Tru1I	TTAA	3 cut(s)	378, 797, 847
Tru9I	TTAA	3 cut(s)	378, 797, 847
TscAI	CASTG	2 cut(s)	265, 280
TseFI	GTSAC	2 cut(s)	133, 170
TseI	GCWGC	5 cut(s)	514, 527, 679, 682, 836
Tsp45I	GTSAC	2 cut(s)	133, 170
TspDTI	ATGAA	4 cut(s)	43, 460, 468, 738
TspGWI	ACGGA	2 cut(s)	135, 300
TspRI	CASTG	2 cut(s)	265, 280
XapI	RAATTY	1 cut(s)	325
XmnI	GAANNNNTTC	2 cut(s)	35, 446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.