MD15G1133800.v1.1

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009002
Physical Location & Seq
Reverse (-)
9725184 .. 9729059
3876 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1133800.v1.1.491

Sequence Viewer

Length: 1692 bp
ATGTCGTCTTCTTCTTCTCTTCACGGTCCAAACACTGCTCCCATGTCTGCCTCATCCAACGGTAACCCTAATAGTAGTGAAAATAATGATATTGTGAGTGATCATCATCATGTGTCGGTTGTGATAGTAGGTGCTGGTCCGTCTGGCATTGCGACGTCCGCATTGCTCAACTCCCATTCAGTACCAAACCTTCTGTTTGAAAGGGAAGACTGTTGCGCTTCCCTTTGGAAGAAACGGTCCTACGATCGTCTCAATCTTCACTTAGCAAAGGGGTACTGCTCTTTGCCACTCATGCCACACTCCTTCAGGACTTCCACCTTCATGTCCAAATACGATTTCATCGCCTACTTAGATGAGTATGTCTCACGGTTTAACGTGAAACCTCGTTACTGTCGTCATGTGGATTCCGCTGTTTGCGATGAAGATGAGGAGATATGGAAGGTGAAAGTGACTGGTCCGGACCAGTACGAGTACTACTCTTCAGATTTCTTGGTTGTCGCAACAGGAGAAAATAACCAGGCTATTATTCCGGCGGACTTGCCTGGACTTGAGTCATTCACAGGAAAAGTTGTTCATGCATGCGACTATAAGAACGGGGAAAGTTTTAAAGACAAACAAGTTTTGGTCATTGGCTGTGGAAACTCTGGTATGGAAATTAGCAACGACCTTGCAGACAATGGAGCGCATGCATCCCTTGTTATTAGAAGCCAAATGCATGTCCTCAGCAAAGAGATGGTAGCTATAGGAATGCTTCTGTTGGATTACTTGCCAGTGAAAATAGTGGACAAGTTTGTCTCATTCCTTGCCAAGTTTAGGTATCAGGATTTGTCCAGCTATGGCATTCATCGCCCGGACCAAGGCCCTTTTCTCTTTAAAGCACTTACTGGAAAGAGGCCAGTGATTGATCGTGGAACCATCAACAAAATTCGATCCAAACAGATCAAGATGAAGAAGAAGGAAGAAGGAAGATGGAGAAGAAGGGGAAGGAAGAAGGAGAAGAAGAAGGAGGAAGGAAGAAGGAGAAGAAGACGGAGGAAGAAGGAGGAAGAAAAAAAAAGTTGTAGTCGGAGGAAGAAGAAGAAGAAGAAGGAAGAAGGAGGAAGGAGGAGGAAGAAGAAGAAAAAAAGAAAAAAAAAACAACCTTCATCTTCCCCAGATTCGGAAGAAGAAGAAGAAGAAAGAGGAAGAAGGAGGAAGAAGGAAAAAAAAAAAAAAAAAAAAAACCTTCCCCAGATTCGGAGGAAGAAGAAGAAGACGGAGAAGGAGAAGAAGGGGAAGGAAGAAGGAGGAAGGAAGAAGAAGAAGGATGAAGAAAAAAAAAGTTGCAAAAGAAGGAAGAAGAAGAAAGAAGAAAAGAAAAGAAAAGAAAAAAACGTGGATGACACGTGGTGCCCAGTTTTTCCAGGAATAGTGAGGATAAACTCCAACTCTGTTGAGTTCAACAACGGGGCCCGACAGAGTTTTGATGCCATTCTGTTGGCAACTGGTTATAAAAGTGTAGCACATAAGTGGCTCAAGGACTATAAGTACCTCCTTAATGACGATGGAAAGCCTAAAGGAAATTATCCAAACCACTGGAAGGGCGAGAAGGGAGTTTACTGTGTTGGATTAGCAGGGAATGGACTGCCGGGGATTTTCACGGATTCAACGGCTGTGGCCGAGGATATCCATAACCTAATGGCACAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

564

Amino Acids

64.48

Weight (kDa)

9.88

Isoelectric Point (pI)

56.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 39 - 237 2.4e-12 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 40 - 244 1.5e-16 Flavin-binding monooxygenase-like
Pyr_redox_3 PF13738 41 - 237 5.7e-15 Pyridine nucleotide-disulphide oxidoreductase
Lys_Orn_oxgnase PF13434 100 - 236 2.1e-11 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1491
AasI GACNNNNNNGTC 1 cut(s) 791
AatII GACGTC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 1389
AccIII TCCGGA 1 cut(s) 457
AciI CCGC 3 cut(s) 159, 408, 533
AclWI GGATC 1 cut(s) 924
AcoI YGGCCR 1 cut(s) 1656
AcsI RAATTY 1 cut(s) 924
AcuI CTGAAG 2 cut(s) 289, 465
AcvI CACGTG 1 cut(s) 1386
AcyI GRCGYC 1 cut(s) 155
AdeI CACNNNGTG 1 cut(s) 1389
AfaI GTAC 5 cut(s) 183, 275, 467, 473, 1529
AfiI CCNNNNNNNGG 5 cut(s) 813, 857, 1159, 1236, 1579
AflIII ACRYGT 1 cut(s) 1383
AgsI TTSAA 3 cut(s) 200, 1441, 1647
AjnI CCWGG 3 cut(s) 516, 541, 1402
AjuI GAANNNNNNNTTGG 4 cut(s) 320, 352, 849, 881
AleI CACNNNNGTG 1 cut(s) 1507
AloI GAACNNNNNNTCC 2 cut(s) 555, 587
AluBI AGCT 2 cut(s) 740, 834
AluI AGCT 2 cut(s) 740, 834
Alw26I GTCTC 3 cut(s) 254, 367, 799
AlwI GGATC 1 cut(s) 924
Aor13HI TCCGGA 1 cut(s) 457
AoxI GGCC 4 cut(s) 859, 893, 1449, 1656
ApaI GGGCCC 1 cut(s) 1453
ApoI RAATTY 1 cut(s) 924
AspLEI GCGC 2 cut(s) 218, 685
AspS9I GGNCC 9 cut(s) 26, 137, 237, 455, 460, 853, 860, 1449, 1450
AsuC2I CCSGG 2 cut(s) 851, 1629
AsuHPI GGTGA 1 cut(s) 454
AvaII GGWCC 6 cut(s) 26, 137, 237, 455, 460, 853
BaeGI GKGCMC 2 cut(s) 1394, 1453
BanI GGYRCC 1 cut(s) 1389
BanII GRGCYC 1 cut(s) 1453
BarI GAAGNNNNNNTAC 4 cut(s) 174, 206, 1580, 1612
BbrPI CACGTG 1 cut(s) 1386
BbsI GAAGAC 3 cut(s) 213, 1033, 1259
BbvCI CCTCAGC 1 cut(s) 722
BccI CCATC 4 cut(s) 727, 923, 963, 1538
BceAI ACGGC 1 cut(s) 1665
BciT130I CCWGG 3 cut(s) 518, 543, 1404
BclI TGATCA 1 cut(s) 100
BcnI CCSGG 2 cut(s) 851, 1629
BcoDI GTCTC 3 cut(s) 254, 367, 799
BfmI CTRYAG 1 cut(s) 741
BmcAI AGTACT 1 cut(s) 473
Bme1390I CCNGG 5 cut(s) 518, 543, 851, 1404, 1629
Bme18I GGWCC 6 cut(s) 26, 137, 237, 455, 460, 853
BmgT120I GGNCC 9 cut(s) 26, 137, 237, 455, 460, 853, 860, 1449, 1450
BmiI GGNNCC 4 cut(s) 913, 1391, 1450, 1451
BmrFI CCNGG 5 cut(s) 518, 543, 851, 1404, 1629
BmrI ACTGGG 1 cut(s) 1388
BmsI GCATC 2 cut(s) 698, 1456
BmuI ACTGGG 1 cut(s) 1388
BoxI GACNNNNGTC 1 cut(s) 550
BpiI GAAGAC 3 cut(s) 213, 1033, 1259
BplI GAGNNNNNCTC 4 cut(s) 347, 379, 461, 493
Bpu10I CCTNAGC 1 cut(s) 722
BpuEI CTTGAG 2 cut(s) 569, 1499
BpuMI CCSGG 2 cut(s) 851, 1629
BsaAI YACGTR 1 cut(s) 1386
BsaBI GATNNNNATC 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 155
BsaJI CCNNGG 3 cut(s) 856, 1628, 1659
BsaWI WCCGGW 1 cut(s) 457
Bsc4I CCNNNNNNNGG 5 cut(s) 813, 857, 1159, 1236, 1579
Bse1I ACTGG 8 cut(s) 457, 463, 770, 889, 896, 1394, 1489, 1580
Bse3DI GCAATG 2 cut(s) 147, 161
Bse8I GATNNNNATC 1 cut(s) 105
BseAI TCCGGA 1 cut(s) 457
BseBI CCWGG 3 cut(s) 518, 543, 1404
BseDI CCNNGG 3 cut(s) 856, 1628, 1659
BseGI GGATG 4 cut(s) 53, 689, 1312, 1384
BseJI GATNNNNATC 1 cut(s) 105
BseLI CCNNNNNNNGG 5 cut(s) 813, 857, 1159, 1236, 1579
BseMI GCAATG 2 cut(s) 147, 161
BseMII CTCAG 1 cut(s) 736
BseNI ACTGG 8 cut(s) 457, 463, 770, 889, 896, 1394, 1489, 1580
BseRI GAGGAG 2 cut(s) 443, 1120
BseSI GKGCMC 2 cut(s) 1394, 1453
Bsh1285I CGRYCG 1 cut(s) 247
BshFI GGCC 4 cut(s) 861, 895, 1451, 1658
BshNI GGYRCC 1 cut(s) 1389
BsiEI CGRYCG 1 cut(s) 247
BsiSI CCGG 4 cut(s) 458, 530, 851, 1628
BslI CCNNNNNNNGG 5 cut(s) 813, 857, 1159, 1236, 1579
BsmAI GTCTC 3 cut(s) 254, 367, 799
BsmBI CGTCTC 1 cut(s) 254
BsmI GAATGC 2 cut(s) 753, 840
BsnI GGCC 4 cut(s) 861, 895, 1451, 1658
Bsp120I GGGCCC 1 cut(s) 1449
Bsp1286I GDGCHC 2 cut(s) 1394, 1453
Bsp13I TCCGGA 1 cut(s) 457
Bsp143I GATC 5 cut(s) 100, 244, 904, 929, 939
BspACI CCGC 3 cut(s) 159, 408, 533
BspANI GGCC 4 cut(s) 861, 895, 1451, 1658
BspCNI CTCAG 1 cut(s) 735
BspEI TCCGGA 1 cut(s) 457
BspLI GGNNCC 4 cut(s) 913, 1391, 1450, 1451
BspPI GGATC 1 cut(s) 924
BspT107I GGYRCC 1 cut(s) 1389
BsrDI GCAATG 2 cut(s) 147, 161
BsrI ACTGG 8 cut(s) 457, 463, 770, 889, 896, 1394, 1489, 1580
BssECI CCNNGG 3 cut(s) 856, 1628, 1659
BssMI GATC 5 cut(s) 100, 244, 904, 929, 939
BssNI GRCGYC 1 cut(s) 155
BssT1I CCWWGG 1 cut(s) 856
Bst2UI CCWGG 3 cut(s) 518, 543, 1404
Bst4CI ACNGT 7 cut(s) 26, 62, 212, 237, 369, 392, 1601
Bst6I CTCTTC 2 cut(s) 24, 484
BstACI GRCGYC 1 cut(s) 155
BstBAI YACGTR 1 cut(s) 1386
BstC8I GCNNGC 2 cut(s) 580, 687
BstDEI CTNAG 3 cut(s) 262, 349, 722
BstEII GGTNACC 1 cut(s) 62
BstF5I GGATG 4 cut(s) 53, 689, 1312, 1384
BstHHI GCGC 2 cut(s) 218, 685
BstKTI GATC 5 cut(s) 103, 247, 907, 932, 942
BstMAI GTCTC 3 cut(s) 254, 367, 799
BstMBI GATC 5 cut(s) 100, 244, 904, 929, 939
BstMCI CGRYCG 1 cut(s) 247
BstMWI GCNNNNNNNGC 3 cut(s) 158, 292, 846
BstNI CCWGG 3 cut(s) 518, 543, 1404
BstNSI RCATGY 3 cut(s) 582, 689, 719
BstPAI GACNNNNGTC 1 cut(s) 550
BstPI GGTNACC 1 cut(s) 62
BstSCI CCNGG 5 cut(s) 516, 541, 849, 1402, 1627
BstSFI CTRYAG 1 cut(s) 741
BstSLI GKGCMC 2 cut(s) 1394, 1453
BstV2I GAAGAC 3 cut(s) 213, 1033, 1259
BstXI CCANNNNNNTGG 2 cut(s) 1477, 1575
BsuRI GGCC 4 cut(s) 861, 895, 1451, 1658
BtgZI GCGATG 3 cut(s) 325, 432, 830
BtsCI GGATG 4 cut(s) 53, 689, 1312, 1384
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 4 cut(s) 33, 777, 903, 1573
Cac8I GCNNGC 2 cut(s) 580, 687
CfoI GCGC 2 cut(s) 218, 685
Cfr13I GGNCC 9 cut(s) 26, 137, 237, 455, 460, 853, 860, 1449, 1450
Csp6I GTAC 5 cut(s) 182, 274, 466, 472, 1528
CspCI CAANNNNNGTGG 2 cut(s) 1635, 1670
CviAII CATG 9 cut(s) 43, 110, 292, 322, 398, 575, 579, 686, 716
CviQI GTAC 5 cut(s) 182, 274, 466, 472, 1528
DdeI CTNAG 3 cut(s) 262, 349, 722
DpnI GATC 5 cut(s) 102, 246, 906, 931, 941
DpnII GATC 5 cut(s) 100, 244, 904, 929, 939
DraI TTTAAA 2 cut(s) 607, 874
DraIII CACNNNGTG 1 cut(s) 1389
DrdI GACNNNNNNGTC 1 cut(s) 791
DseDI GACNNNNNNGTC 1 cut(s) 791
EaeI YGGCCR 1 cut(s) 1656
Eam1104I CTCTTC 2 cut(s) 24, 484
EarI CTCTTC 2 cut(s) 24, 484
EciI GGCGGA 1 cut(s) 548
Eco130I CCWWGG 1 cut(s) 856
Eco24I GRGCYC 1 cut(s) 1453
Eco32I GATATC 1 cut(s) 1666
Eco47I GGWCC 6 cut(s) 26, 137, 237, 455, 460, 853
Eco57I CTGAAG 2 cut(s) 289, 465
Eco72I CACGTG 1 cut(s) 1386
Eco91I GGTNACC 1 cut(s) 62
EcoO109I RGGNCCY 2 cut(s) 860, 1449
EcoO65I GGTNACC 1 cut(s) 62
EcoRII CCWGG 3 cut(s) 516, 541, 1402
EcoRV GATATC 1 cut(s) 1666
EcoT14I CCWWGG 1 cut(s) 856
EcoT22I ATGCAT 3 cut(s) 580, 691, 717
EcoT38I GRGCYC 1 cut(s) 1453
ErhI CCWWGG 1 cut(s) 856
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 9 cut(s) 46, 113, 295, 325, 401, 578, 582, 689, 719
FatI CATG 9 cut(s) 42, 109, 291, 321, 397, 574, 578, 685, 715
FbaI TGATCA 1 cut(s) 100
FokI GGATG 4 cut(s) 40, 676, 1319, 1391
FriOI GRGCYC 1 cut(s) 1453
GlaI GCGC 2 cut(s) 217, 684
HaeIII GGCC 4 cut(s) 861, 895, 1451, 1658
HapII CCGG 4 cut(s) 458, 530, 851, 1628
HhaI GCGC 2 cut(s) 218, 685
Hin1I GRCGYC 1 cut(s) 155
Hin1II CATG 9 cut(s) 46, 113, 295, 325, 401, 578, 582, 689, 719
Hin6I GCGC 2 cut(s) 216, 683
HinP1I GCGC 2 cut(s) 216, 683
HinfI GANTC 5 cut(s) 404, 551, 1157, 1234, 1643
HpaII CCGG 4 cut(s) 458, 530, 851, 1628
HphI GGTGA 1 cut(s) 454
Hpy166II GTNNAC 2 cut(s) 784, 1597
Hpy188I TCNGA 4 cut(s) 484, 1068, 1162, 1239
Hpy188III TCNNGA 4 cut(s) 307, 458, 821, 943
Hpy8I GTNNAC 2 cut(s) 784, 1597
Hpy99I CGWCG 1 cut(s) 157
HpyCH4III ACNGT 7 cut(s) 26, 62, 212, 237, 369, 392, 1601
HpyCH4IV ACGT 4 cut(s) 155, 375, 1374, 1385
HpyCH4V TGCA 5 cut(s) 578, 671, 689, 715, 1326
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 292, 846
HpyF3I CTNAG 3 cut(s) 262, 349, 722
HpySE526I ACGT 4 cut(s) 155, 375, 1374, 1385
Hsp92I GRCGYC 1 cut(s) 155
Hsp92II CATG 9 cut(s) 46, 113, 295, 325, 401, 578, 582, 689, 719
HspAI GCGC 2 cut(s) 216, 683
Kpn2I TCCGGA 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 100
Kzo9I GATC 5 cut(s) 100, 244, 904, 929, 939
LmnI GCTCC 2 cut(s) 43, 680
LweI GCATC 2 cut(s) 698, 1456
MaeII ACGT 4 cut(s) 155, 375, 1374, 1385
MaeIII GTNAC 3 cut(s) 62, 386, 448
MalI GATC 5 cut(s) 102, 246, 906, 931, 941
MboI GATC 5 cut(s) 100, 244, 904, 929, 939
MhlI GDGCHC 2 cut(s) 1394, 1453
MluCI AATT 3 cut(s) 654, 924, 1561
MlyI GAGTC 1 cut(s) 560
MmeI TCCRAC 5 cut(s) 81, 738, 1046, 1449, 1585
Mph1103I ATGCAT 3 cut(s) 580, 691, 717
MroI TCCGGA 1 cut(s) 457
MseI TTAA 4 cut(s) 372, 606, 873, 1536
MslI CAYNNNNRTG 3 cut(s) 108, 320, 1507
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 4 cut(s) 458, 530, 851, 1628
MspR9I CCNGG 5 cut(s) 518, 543, 851, 1404, 1629
Mva1269I GAATGC 2 cut(s) 753, 840
MvaI CCWGG 3 cut(s) 518, 543, 1404
MwoI GCNNNNNNNGC 3 cut(s) 158, 292, 846
NciI CCSGG 2 cut(s) 851, 1629
NdeII GATC 5 cut(s) 100, 244, 904, 929, 939
NlaIII CATG 9 cut(s) 46, 113, 295, 325, 401, 578, 582, 689, 719
NlaIV GGNNCC 4 cut(s) 913, 1391, 1450, 1451
NmeAIII GCCGAG 1 cut(s) 1684
NmuCI GTSAC 1 cut(s) 448
NsiI ATGCAT 3 cut(s) 580, 691, 717
NspI RCATGY 3 cut(s) 582, 689, 719
OliI CACNNNNGTG 1 cut(s) 1507
PaeI GCATGC 2 cut(s) 582, 689
PcsI WCGNNNNNNNCGW 1 cut(s) 391
PctI GAATGC 2 cut(s) 753, 840
PfeI GAWTC 4 cut(s) 404, 1157, 1234, 1643
PfoI TCCNGGA 1 cut(s) 1402
Ple19I CGATCG 1 cut(s) 247
PleI GAGTC 1 cut(s) 559
PmaCI CACGTG 1 cut(s) 1386
PmlI CACGTG 1 cut(s) 1386
PpsI GAGTC 1 cut(s) 559
Ppu21I YACGTR 1 cut(s) 1386
PshAI GACNNNNGTC 1 cut(s) 550
PsiI TTATAA 1 cut(s) 1491
Psp6I CCWGG 3 cut(s) 516, 541, 1402
PspCI CACGTG 1 cut(s) 1386
PspEI GGTNACC 1 cut(s) 62
PspGI CCWGG 3 cut(s) 516, 541, 1402
PspN4I GGNNCC 4 cut(s) 913, 1391, 1450, 1451
PspOMI GGGCCC 1 cut(s) 1449
PspPI GGNCC 9 cut(s) 26, 137, 237, 455, 460, 853, 860, 1449, 1450
PvuI CGATCG 1 cut(s) 247
RsaI GTAC 5 cut(s) 183, 275, 467, 473, 1529
RsaNI GTAC 5 cut(s) 182, 274, 466, 472, 1528
RseI CAYNNNNRTG 3 cut(s) 108, 320, 1507
SaqAI TTAA 4 cut(s) 372, 606, 873, 1536
Sau3AI GATC 5 cut(s) 100, 244, 904, 929, 939
Sau96I GGNCC 9 cut(s) 26, 137, 237, 455, 460, 853, 860, 1449, 1450
ScaI AGTACT 1 cut(s) 473
SchI GAGTC 1 cut(s) 560
ScrFI CCNGG 5 cut(s) 518, 543, 851, 1404, 1629
SduI GDGCHC 2 cut(s) 1394, 1453
SfaNI GCATC 2 cut(s) 698, 1456
SfcI CTRYAG 1 cut(s) 741
SinI GGWCC 6 cut(s) 26, 137, 237, 455, 460, 853
SmiMI CAYNNNNRTG 3 cut(s) 108, 320, 1507
SmlI CTYRAG 2 cut(s) 548, 1514
SmoI CTYRAG 2 cut(s) 548, 1514
SphI GCATGC 2 cut(s) 582, 689
Sse9I AATT 3 cut(s) 654, 924, 1561
SsiI CCGC 3 cut(s) 159, 408, 533
StyD4I CCNGG 5 cut(s) 516, 541, 849, 1402, 1627
StyI CCWWGG 1 cut(s) 856
TaaI ACNGT 7 cut(s) 26, 62, 212, 237, 369, 392, 1601
TaiI ACGT 4 cut(s) 158, 378, 1377, 1388
TaqI TCGA 1 cut(s) 928
TasI AATT 3 cut(s) 654, 924, 1561
TatI WGTACW 1 cut(s) 471
TfiI GAWTC 4 cut(s) 404, 1157, 1234, 1643
Tru1I TTAA 4 cut(s) 372, 606, 873, 1536
Tru9I TTAA 4 cut(s) 372, 606, 873, 1536
TscAI CASTG 4 cut(s) 40, 777, 903, 1580
TseFI GTSAC 1 cut(s) 448
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 8 cut(s) 310, 328, 435, 563, 833, 962, 1134, 1323
TspGWI ACGGA 4 cut(s) 129, 1045, 1271, 1655
TspRI CASTG 4 cut(s) 40, 777, 903, 1580
VpaK11BI GGWCC 6 cut(s) 26, 137, 237, 455, 460, 853
XapI RAATTY 1 cut(s) 924
XceI RCATGY 3 cut(s) 582, 689, 719
ZraI GACGTC 1 cut(s) 156
ZrmI AGTACT 1 cut(s) 473
Zsp2I ATGCAT 3 cut(s) 580, 691, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.