Rh4CG252000

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009002
Physical Location & Seq
Forward (+)
50177487 .. 50181540
4054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG252000.1

Sequence Viewer

Length: 1197 bp
ATGGAGAACATTGTGTCTGAGAATGCATTGGTGGTGATAGTAGGCGCTGGCCCCTCCGGCATTGCAACCTCAGCGCTACTCAACTCCATGTCGGTGTCAAACATTGTTTTTGAGAGGGAAGACTGTTGTGCTTCCCTTTGGAAGAAACGGTCCTACGACCGTTTGAGTCTTCACTTGGCAAAAAAATTTTGCTCTCTTCCTCTTATGCCGCACCCATTTAGCACTGCAACTTTCATGTCCAAAGACACGTTCATAGACTATGTGGATGAGTATGTTTCCCGTTTTAATGTCAACCCCCGTTATTGTCACAATGTTGAGTCGGCTTTTTACGAAGAAGCTGATCAGAAATGGAAGGTTGAAGTGAAGAACACTAGGGCGGCTGATAAGGAGGTTTCCTTTCAAGTGTACAACGCAGATTTCTTGGTGATTGCCACCGGAGAAAATAGCCAGGCGATTATCCCAGAGTTGCCTGGACTGGAGACCTTTAAAGGAAAGGTTATTCATGCGAGTGATTATAAATGTGGAGCGAGTTTGAAAGATAAGAGTGTGTTGGTTATTGGTTGTGGCAATTCTGGCATGGAGATTAGTAATGATCTCTCGGAGAATGGAGCTCATGCATGCATTGTTGTTAGGAGCCAACTTCATGTACTCAGCAGAGAAATTGTACGGATTGGGATGGTACTGTTGGATTTCCTCCCGGTGAATATGGTGGACAAGTTCGTTTCATTTCTCGCCAAGTTTAGCTACAGTGATCTACCCAACTATGGGATTCATCAACCGACTGAAGGTCCTTTCTTCTATAAAGCACTTACGGGAAAGACTCCAGTAATTGATCGTGGAACTATCAAGAAAATTCGCTCCAGAAAGATTAAGGTTTTCCCAGGAATAGAAAAGATACACGACAATATTGTGGAATTCAAAAATGGTGCCCTCCTGCGTTTTGATGCCATTGTGTTGGCAACTGGTTATCGAAGCGTAGCACACAACTGGCTCAAGGATTATAAGCTCATTCTTGATAATGACGACAAGCCTAAGAACAGGTATCCATACCATTGGAAAGGAGAGAAGGGCCTTTACTGTGTTGGATTTTCTGGGAGAGGGATTCAGGGAATCTCGTCGGATTCTAAGGCTGTTGCTCAAGATATCTATCGAATTTTAATGGCACAGAAGTTCGCTTATGCCCCAGCAATGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

398

Amino Acids

44.54

Weight (kDa)

8.7

Isoelectric Point (pI)

31.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMO-like PF00743 11 - 332 8.6e-22 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 11 - 212 5.1e-14 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 12 - 213 1.8e-17 Pyridine nucleotide-disulphide oxidoreductase
Lys_Orn_oxgnase PF13434 75 - 208 5.2e-12 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 516, 1002
AccB1I GGYRCC 1 cut(s) 926
AciI CCGC 2 cut(s) 209, 377
AcsI RAATTY 4 cut(s) 185, 852, 914, 1152
AcuI CTGAAG 1 cut(s) 804
AfaI GTAC 4 cut(s) 407, 648, 666, 681
AfeI AGCGCT 1 cut(s) 75
AfiI CCNNNNNNNGG 4 cut(s) 764, 765, 785, 1189
AflIII ACRYGT 1 cut(s) 246
AgsI TTSAA 4 cut(s) 359, 401, 535, 919
AhdI GACNNNNNGTC 1 cut(s) 786
AjnI CCWGG 3 cut(s) 447, 469, 880
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
AluBI AGCT 4 cut(s) 338, 611, 744, 1006
AluI AGCT 4 cut(s) 338, 611, 744, 1006
Alw21I GWGCWC 1 cut(s) 613
Alw26I GTCTC 1 cut(s) 473
Aor51HI AGCGCT 1 cut(s) 75
AoxI GGCC 2 cut(s) 49, 1069
ApoI RAATTY 4 cut(s) 185, 852, 914, 1152
AspLEI GCGC 2 cut(s) 47, 76
AspS9I GGNCC 4 cut(s) 50, 150, 788, 1069
AsuC2I CCSGG 1 cut(s) 698
AsuHPI GGTGA 3 cut(s) 46, 436, 712
AvaII GGWCC 2 cut(s) 150, 788
BaeGI GKGCMC 1 cut(s) 931
BanI GGYRCC 1 cut(s) 926
BanII GRGCYC 1 cut(s) 613
BarI GAAGNNNNNNTAC 2 cut(s) 1058, 1090
BbsI GAAGAC 2 cut(s) 126, 161
Bbv12I GWGCWC 1 cut(s) 613
BbvCI CCTCAGC 1 cut(s) 70
BccI CCATC 1 cut(s) 670
BciT130I CCWGG 3 cut(s) 449, 471, 882
BciVI GTATCC 1 cut(s) 1053
BclI TGATCA 1 cut(s) 340
BcnI CCSGG 1 cut(s) 698
BcoDI GTCTC 1 cut(s) 473
BfaI CTAG 1 cut(s) 372
BfmI CTRYAG 1 cut(s) 745
BfoI RGCGCY 2 cut(s) 48, 77
BfuI GTATCC 1 cut(s) 1053
BglI GCCNNNNNGGC 1 cut(s) 57
BisI GCNGC 2 cut(s) 209, 378
BlsI GCNGC 2 cut(s) 210, 379
Bme1390I CCNGG 4 cut(s) 449, 471, 698, 882
Bme18I GGWCC 2 cut(s) 150, 788
BmeRI GACNNNNNGTC 1 cut(s) 786
BmgT120I GGNCC 4 cut(s) 50, 150, 788, 1069
BmiI GGNNCC 3 cut(s) 52, 635, 928
BmrFI CCNGG 4 cut(s) 449, 471, 698, 882
BmsI GCATC 1 cut(s) 934
BpiI GAAGAC 2 cut(s) 126, 161
BpmI CTGGAG 3 cut(s) 497, 807, 844
Bpu10I CCTNAGC 1 cut(s) 70
BpuEI CTTGAG 2 cut(s) 977, 1122
BpuMI CCSGG 1 cut(s) 698
BsaBI GATNNNNATC 1 cut(s) 1146
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 1 cut(s) 880
BsaWI WCCGGW 1 cut(s) 434
Bsc4I CCNNNNNNNGG 4 cut(s) 764, 765, 785, 1189
Bse1I ACTGG 4 cut(s) 480, 824, 967, 992
Bse3DI GCAATG 2 cut(s) 60, 1194
Bse8I GATNNNNATC 1 cut(s) 1146
BseBI CCWGG 3 cut(s) 449, 471, 882
BseDI CCNNGG 1 cut(s) 880
BseGI GGATG 2 cut(s) 271, 681
BseJI GATNNNNATC 1 cut(s) 1146
BseLI CCNNNNNNNGG 4 cut(s) 764, 765, 785, 1189
BseMI GCAATG 2 cut(s) 60, 1194
BseMII CTCAG 3 cut(s) 9, 84, 664
BseNI ACTGG 4 cut(s) 480, 824, 967, 992
BseSI GKGCMC 1 cut(s) 931
BseYI CCCAGC 1 cut(s) 1183
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 2 cut(s) 51, 1071
BshNI GGYRCC 1 cut(s) 926
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 1 cut(s) 613
BsiSI CCGG 3 cut(s) 57, 435, 698
BslI CCNNNNNNNGG 4 cut(s) 764, 765, 785, 1189
BsmAI GTCTC 1 cut(s) 473
BsmI GAATGC 1 cut(s) 28
BsnI GGCC 2 cut(s) 51, 1071
Bso31I GGTCTC 1 cut(s) 473
Bsp1286I GDGCHC 2 cut(s) 613, 931
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 4 cut(s) 340, 592, 751, 832
BspACI CCGC 2 cut(s) 209, 377
BspANI GGCC 2 cut(s) 51, 1071
BspCNI CTCAG 3 cut(s) 10, 83, 663
BspLI GGNNCC 3 cut(s) 52, 635, 928
BspT107I GGYRCC 1 cut(s) 926
BspTNI GGTCTC 1 cut(s) 473
BsrDI GCAATG 2 cut(s) 60, 1194
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 4 cut(s) 480, 824, 967, 992
BssECI CCNNGG 1 cut(s) 880
BssMI GATC 4 cut(s) 340, 592, 751, 832
Bst2UI CCWGG 3 cut(s) 449, 471, 882
Bst4CI ACNGT 6 cut(s) 125, 150, 161, 684, 749, 1079
Bst6I CTCTTC 1 cut(s) 201
BstAUI TGTACA 1 cut(s) 405
BstC8I GCNNGC 2 cut(s) 49, 619
BstDEI CTNAG 5 cut(s) 18, 70, 650, 1032, 1125
BstF5I GGATG 2 cut(s) 271, 681
BstH2I RGCGCY 2 cut(s) 48, 77
BstHHI GCGC 2 cut(s) 47, 76
BstKTI GATC 4 cut(s) 343, 595, 754, 835
BstMAI GTCTC 1 cut(s) 473
BstMBI GATC 4 cut(s) 340, 592, 751, 832
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 3 cut(s) 57, 71, 573
BstNI CCWGG 3 cut(s) 449, 471, 882
BstNSI RCATGY 1 cut(s) 621
BstSCI CCNGG 4 cut(s) 447, 469, 696, 880
BstSFI CTRYAG 1 cut(s) 745
BstSLI GKGCMC 1 cut(s) 931
BstV2I GAAGAC 2 cut(s) 126, 161
BstXI CCANNNNNNTGG 2 cut(s) 955, 1053
BsuI GTATCC 1 cut(s) 1053
BsuRI GGCC 2 cut(s) 51, 1071
BtsCI GGATG 2 cut(s) 271, 681
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 2 cut(s) 222, 754
Cac8I GCNNGC 2 cut(s) 49, 619
CfoI GCGC 2 cut(s) 47, 76
Cfr13I GGNCC 4 cut(s) 50, 150, 788, 1069
Csp6I GTAC 4 cut(s) 406, 647, 665, 680
CviAII CATG 7 cut(s) 88, 235, 503, 577, 614, 618, 644
CviQI GTAC 4 cut(s) 406, 647, 665, 680
DdeI CTNAG 5 cut(s) 18, 70, 650, 1032, 1125
DpnI GATC 4 cut(s) 342, 594, 753, 834
DpnII GATC 4 cut(s) 340, 592, 751, 832
DraI TTTAAA 1 cut(s) 487
DriI GACNNNNNGTC 1 cut(s) 786
Eam1104I CTCTTC 1 cut(s) 201
Eam1105I GACNNNNNGTC 1 cut(s) 786
EarI CTCTTC 1 cut(s) 201
Ecl136II GAGCTC 1 cut(s) 611
Eco24I GRGCYC 1 cut(s) 613
Eco31I GGTCTC 1 cut(s) 473
Eco32I GATATC 1 cut(s) 1144
Eco47I GGWCC 2 cut(s) 150, 788
Eco47III AGCGCT 1 cut(s) 75
Eco53kI GAGCTC 1 cut(s) 611
Eco57I CTGAAG 1 cut(s) 804
EcoICRI GAGCTC 1 cut(s) 611
EcoO109I RGGNCCY 2 cut(s) 788, 1069
EcoRI GAATTC 1 cut(s) 914
EcoRII CCWGG 3 cut(s) 447, 469, 880
EcoRV GATATC 1 cut(s) 1144
EcoT22I ATGCAT 3 cut(s) 28, 619, 623
EcoT38I GRGCYC 1 cut(s) 613
FaeI CATG 7 cut(s) 91, 238, 506, 580, 617, 621, 647
FatI CATG 7 cut(s) 87, 234, 502, 576, 613, 617, 643
FbaI TGATCA 1 cut(s) 340
Fnu4HI GCNGC 2 cut(s) 209, 378
FokI GGATG 2 cut(s) 278, 688
FriOI GRGCYC 1 cut(s) 613
Fsp4HI GCNGC 2 cut(s) 209, 378
FspBI CTAG 1 cut(s) 372
GlaI GCGC 2 cut(s) 46, 75
GluI GCNGC 2 cut(s) 209, 378
GsaI CCCAGC 1 cut(s) 1187
GsuI CTGGAG 3 cut(s) 497, 807, 844
HaeII RGCGCY 2 cut(s) 48, 77
HaeIII GGCC 2 cut(s) 51, 1071
HapII CCGG 3 cut(s) 57, 435, 698
HhaI GCGC 2 cut(s) 47, 76
Hin1II CATG 7 cut(s) 91, 238, 506, 580, 617, 621, 647
Hin6I GCGC 2 cut(s) 45, 74
HinP1I GCGC 2 cut(s) 45, 74
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 7 cut(s) 166, 317, 769, 820, 1102, 1110, 1121
HpaII CCGG 3 cut(s) 57, 435, 698
HphI GGTGA 3 cut(s) 46, 436, 712
Hpy166II GTNNAC 3 cut(s) 292, 406, 712
Hpy188I TCNGA 4 cut(s) 19, 345, 601, 1120
Hpy188III TCNNGA 4 cut(s) 847, 861, 1013, 1139
Hpy8I GTNNAC 3 cut(s) 292, 406, 712
Hpy99I CGWCG 1 cut(s) 1120
HpyAV CCTTC 3 cut(s) 346, 779, 1060
HpyCH4III ACNGT 6 cut(s) 125, 150, 161, 684, 749, 1079
HpyCH4IV ACGT 1 cut(s) 248
HpyCH4V TGCA 5 cut(s) 26, 65, 227, 617, 621
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 71, 573
HpyF3I CTNAG 5 cut(s) 18, 70, 650, 1032, 1125
HpySE526I ACGT 1 cut(s) 248
Hsp92II CATG 7 cut(s) 91, 238, 506, 580, 617, 621, 647
HspAI GCGC 2 cut(s) 45, 74
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 4 cut(s) 340, 592, 751, 832
LmnI GCTCC 4 cut(s) 524, 608, 633, 863
LweI GCATC 1 cut(s) 934
MaeI CTAG 1 cut(s) 372
MaeII ACGT 1 cut(s) 248
MaeIII GTNAC 1 cut(s) 305
MalI GATC 4 cut(s) 342, 594, 753, 834
MboI GATC 4 cut(s) 340, 592, 751, 832
MboII GAAGA 7 cut(s) 131, 154, 161, 188, 344, 376, 787
MhlI GDGCHC 2 cut(s) 613, 931
MluCI AATT 7 cut(s) 185, 568, 660, 828, 852, 914, 1152
MlyI GAGTC 3 cut(s) 175, 326, 814
MmeI TCCRAC 3 cut(s) 666, 1063, 1098
MnlI CCTC 8 cut(s) 64, 79, 108, 210, 382, 704, 941, 1091
Mph1103I ATGCAT 3 cut(s) 28, 619, 623
MseI TTAA 4 cut(s) 285, 486, 870, 1157
MslI CAYNNNNRTG 2 cut(s) 92, 507
MspI CCGG 3 cut(s) 57, 435, 698
MspR9I CCNGG 4 cut(s) 449, 471, 698, 882
Mva1269I GAATGC 1 cut(s) 28
MvaI CCWGG 3 cut(s) 449, 471, 882
MwoI GCNNNNNNNGC 3 cut(s) 57, 71, 573
NciI CCSGG 1 cut(s) 698
NdeII GATC 4 cut(s) 340, 592, 751, 832
NlaIII CATG 7 cut(s) 91, 238, 506, 580, 617, 621, 647
NlaIV GGNNCC 3 cut(s) 52, 635, 928
NmuCI GTSAC 1 cut(s) 305
NsiI ATGCAT 3 cut(s) 28, 619, 623
NspI RCATGY 1 cut(s) 621
PaeI GCATGC 1 cut(s) 621
PctI GAATGC 1 cut(s) 28
PfeI GAWTC 4 cut(s) 769, 1102, 1110, 1121
PkrI GCNGC 2 cut(s) 210, 379
PleI GAGTC 3 cut(s) 174, 325, 814
PpsI GAGTC 3 cut(s) 174, 325, 814
PpuMI RGGWCCY 1 cut(s) 788
PsiI TTATAA 2 cut(s) 516, 1002
Psp124BI GAGCTC 1 cut(s) 613
Psp5II RGGWCCY 1 cut(s) 788
Psp6I CCWGG 3 cut(s) 447, 469, 880
PspFI CCCAGC 1 cut(s) 1183
PspGI CCWGG 3 cut(s) 447, 469, 880
PspN4I GGNNCC 3 cut(s) 52, 635, 928
PspPI GGNCC 4 cut(s) 50, 150, 788, 1069
PspPPI RGGWCCY 1 cut(s) 788
RsaI GTAC 4 cut(s) 407, 648, 666, 681
RsaNI GTAC 4 cut(s) 406, 647, 665, 680
RseI CAYNNNNRTG 2 cut(s) 92, 507
SacI GAGCTC 1 cut(s) 613
SaqAI TTAA 4 cut(s) 285, 486, 870, 1157
SatI GCNGC 2 cut(s) 209, 378
Sau3AI GATC 4 cut(s) 340, 592, 751, 832
Sau96I GGNCC 4 cut(s) 50, 150, 788, 1069
SchI GAGTC 3 cut(s) 175, 326, 814
ScrFI CCNGG 4 cut(s) 449, 471, 698, 882
SduI GDGCHC 2 cut(s) 613, 931
SfaNI GCATC 1 cut(s) 934
SfcI CTRYAG 1 cut(s) 745
SinI GGWCC 2 cut(s) 150, 788
SmiMI CAYNNNNRTG 2 cut(s) 92, 507
SmlI CTYRAG 2 cut(s) 992, 1137
SmoI CTYRAG 2 cut(s) 992, 1137
SphI GCATGC 1 cut(s) 621
Sse9I AATT 7 cut(s) 185, 568, 660, 828, 852, 914, 1152
SsiI CCGC 2 cut(s) 209, 377
SspI AATATT 1 cut(s) 907
SspMI CTAG 1 cut(s) 372
SstI GAGCTC 1 cut(s) 613
StyD4I CCNGG 4 cut(s) 447, 469, 696, 880
TaaI ACNGT 6 cut(s) 125, 150, 161, 684, 749, 1079
TaiI ACGT 1 cut(s) 251
TaqI TCGA 2 cut(s) 970, 1150
TasI AATT 7 cut(s) 185, 568, 660, 828, 852, 914, 1152
TatI WGTACW 2 cut(s) 405, 646
TauI GCSGC 2 cut(s) 211, 380
TfiI GAWTC 4 cut(s) 769, 1102, 1110, 1121
Tru1I TTAA 4 cut(s) 285, 486, 870, 1157
Tru9I TTAA 4 cut(s) 285, 486, 870, 1157
TscAI CASTG 2 cut(s) 229, 754
TseFI GTSAC 1 cut(s) 305
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 6 cut(s) 223, 241, 491, 632, 714, 761
TspGWI ACGGA 1 cut(s) 682
TspRI CASTG 2 cut(s) 229, 754
VpaK11BI GGWCC 2 cut(s) 150, 788
XapI RAATTY 4 cut(s) 185, 852, 914, 1152
XceI RCATGY 1 cut(s) 621
XspI CTAG 1 cut(s) 372
Zsp2I ATGCAT 3 cut(s) 28, 619, 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.