RchiOBHm_Chr4g0422171

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009002
Physical Location & Seq
Forward (+)
47825198 .. 47826984
1787 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39177

Sequence Viewer

Length: 870 bp
ATGGACGAGTACGTTTCCCGTTTCAACGTCAATCCCTATTACTGTCACAATGTTGAGTCGGCTTTTTACGAAGAAGCTGATAAAAAATGGAAGGTTGAAGTGAAGAACACTAGGGTGACTGATAAGGAGGTTTGCCCCCAAGTGTACTATGCGGATTTCTTAGTGGTGGCCACCGGAGAAAATAGCCAGGCAATTATCCCAGAGTTGCCTGGACTGGAGACCTTTAATGGAAAGGTTATTCATGCGAGTGATTATAAATGCGGAGCGAGTTTTAGAGATAAGAGTGTGTTGGTCATTGGTTGTGGCAATTCTGGCATGGAGATTAGTACAGAAATTGTACGAATTGGGATGATACTGTTGAATTTCCTCCCGGTGAATATGGTAGACAGGTTCGTTTCATTTCTTGCCATGTTTAGCTACAGTGATCTGCCCAGCTATGGGATTCATCAACCAGCTGAAGGCCCTTTTTTCTACAAAACACTTACTGGAAAGACTCTGGTAATCGATCGTGGAACTATCAAGAAAATTCGCTCCCAAAAGATTAAGGTTTTCCCAGGAATAGAGAAGATACACAACAATATTGTGGAATTCAAAAATGGTGCCCTCCAGCGTTTCGATGCCATTGTGTTGGCAACTGGTTATCGAAGCGTTGCGCACAACTGGCTCAAGGATTATAAGTTCATTTTTAATGAGGATGACAAGCCTAAGAACAAGTATCCAAACCACTGGAAAGGAGAAAAGGGCGTCTACTGTGTTGGATTCACAGGGAGAGGAATTCAAGGGATTTCGTCGGATTCTAAGGCTGTTGCTGAAGATATCTATCAGCTTGTAATGGCACAGAAATCCACTTATGCTGCAAGAATGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

289

Amino Acids

32.72

Weight (kDa)

8.61

Isoelectric Point (pI)

27.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lys_Orn_oxgnase PF13434 8 - 114 1.8e-07 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Pyr_redox_2 PF07992 28 - 130 2e-08 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 39 - 115 6.4e-13 Flavin-binding monooxygenase-like
Pyr_redox_3 PF13738 47 - 116 3.9e-09 Pyridine nucleotide-disulphide oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 255, 675
Acc16I TGCGCA 1 cut(s) 654
AccB1I GGYRCC 1 cut(s) 599
AccI GTMKAC 2 cut(s) 384, 747
AciI CCGC 2 cut(s) 152, 261
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 4 cut(s) 361, 525, 587, 774
AcuI CTGAAG 2 cut(s) 477, 831
AcyI GRCGYC 1 cut(s) 744
AfaI GTAC 4 cut(s) 11, 146, 328, 339
AfiI CCNNNNNNNGG 3 cut(s) 437, 438, 458
AgsI TTSAA 5 cut(s) 25, 98, 361, 592, 779
AjnI CCWGG 3 cut(s) 186, 208, 553
AleI CACNNNNGTG 1 cut(s) 113
AloI GAACNNNNNNTCC 2 cut(s) 662, 694
AluBI AGCT 5 cut(s) 77, 417, 435, 455, 826
AluI AGCT 5 cut(s) 77, 417, 435, 455, 826
Alw26I GTCTC 1 cut(s) 212
AoxI GGCC 2 cut(s) 168, 460
ApeKI GCWGC 1 cut(s) 854
ApoI RAATTY 4 cut(s) 361, 525, 587, 774
AspLEI GCGC 1 cut(s) 655
AspS9I GGNCC 1 cut(s) 461
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 2 cut(s) 127, 385
BaeGI GKGCMC 1 cut(s) 604
BalI TGGCCA 1 cut(s) 170
BanI GGYRCC 1 cut(s) 599
BbvI GCAGC 1 cut(s) 841
BciT130I CCWGG 3 cut(s) 188, 210, 555
BciVI GTATCC 1 cut(s) 726
BcnI CCSGG 1 cut(s) 371
BcoDI GTCTC 1 cut(s) 212
BfaI CTAG 1 cut(s) 111
BfmI CTRYAG 1 cut(s) 418
BfuI GTATCC 1 cut(s) 726
BisI GCNGC 1 cut(s) 855
BlsI GCNGC 1 cut(s) 856
Bme1390I CCNGG 4 cut(s) 188, 210, 371, 555
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 1 cut(s) 601
BmrFI CCNGG 4 cut(s) 188, 210, 371, 555
BmsI GCATC 1 cut(s) 607
BpmI CTGGAG 2 cut(s) 236, 590
BpuEI CTTGAG 1 cut(s) 650
BpuMI CCSGG 1 cut(s) 371
Bsa29I ATCGAT 1 cut(s) 504
BsaBI GATNNNNATC 1 cut(s) 819
BsaHI GRCGYC 1 cut(s) 744
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 553
BsaWI WCCGGW 1 cut(s) 173
Bsc4I CCNNNNNNNGG 3 cut(s) 437, 438, 458
Bse1I ACTGG 5 cut(s) 219, 490, 640, 665, 731
Bse8I GATNNNNATC 1 cut(s) 819
BseBI CCWGG 3 cut(s) 188, 210, 555
BseCI ATCGAT 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 553
BseGI GGATG 2 cut(s) 354, 700
BseJI GATNNNNATC 1 cut(s) 819
BseLI CCNNNNNNNGG 3 cut(s) 437, 438, 458
BseNI ACTGG 5 cut(s) 219, 490, 640, 665, 731
BseSI GKGCMC 1 cut(s) 604
BseXI GCAGC 1 cut(s) 841
BseYI CCCAGC 1 cut(s) 431
Bsh1285I CGRYCG 1 cut(s) 508
BshFI GGCC 2 cut(s) 170, 462
BshNI GGYRCC 1 cut(s) 599
BshVI ATCGAT 1 cut(s) 504
BsiEI CGRYCG 1 cut(s) 508
BsiSI CCGG 2 cut(s) 174, 371
BslI CCNNNNNNNGG 3 cut(s) 437, 438, 458
BsmAI GTCTC 1 cut(s) 212
BsnI GGCC 2 cut(s) 170, 462
Bso31I GGTCTC 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 604
Bsp143I GATC 2 cut(s) 424, 505
BspACI CCGC 2 cut(s) 152, 261
BspANI GGCC 2 cut(s) 170, 462
BspDI ATCGAT 1 cut(s) 504
BspLI GGNNCC 1 cut(s) 601
BspT107I GGYRCC 1 cut(s) 599
BspTNI GGTCTC 1 cut(s) 212
BsrI ACTGG 5 cut(s) 219, 490, 640, 665, 731
BssECI CCNNGG 1 cut(s) 553
BssMI GATC 2 cut(s) 424, 505
BssNI GRCGYC 1 cut(s) 744
Bst2UI CCWGG 3 cut(s) 188, 210, 555
Bst4CI ACNGT 4 cut(s) 44, 357, 422, 752
BstACI GRCGYC 1 cut(s) 744
BstDEI CTNAG 3 cut(s) 160, 705, 798
BstF5I GGATG 2 cut(s) 354, 700
BstHHI GCGC 1 cut(s) 655
BstKTI GATC 2 cut(s) 427, 508
BstMAI GTCTC 1 cut(s) 212
BstMBI GATC 2 cut(s) 424, 505
BstMCI CGRYCG 1 cut(s) 508
BstMWI GCNNNNNNNGC 2 cut(s) 312, 661
BstNI CCWGG 3 cut(s) 188, 210, 555
BstSCI CCNGG 4 cut(s) 186, 208, 369, 553
BstSFI CTRYAG 1 cut(s) 418
BstSLI GKGCMC 1 cut(s) 604
BstV1I GCAGC 1 cut(s) 841
BstXI CCANNNNNNTGG 2 cut(s) 628, 726
Bsu15I ATCGAT 1 cut(s) 504
BsuI GTATCC 1 cut(s) 726
BsuRI GGCC 2 cut(s) 170, 462
BsuTUI ATCGAT 1 cut(s) 504
BtsCI GGATG 2 cut(s) 354, 700
BtsIMutI CAGTG 2 cut(s) 427, 724
CfoI GCGC 1 cut(s) 655
Cfr13I GGNCC 1 cut(s) 461
ClaI ATCGAT 1 cut(s) 504
CseI GACGC 1 cut(s) 733
Csp6I GTAC 4 cut(s) 10, 145, 327, 338
CviAII CATG 3 cut(s) 242, 316, 409
CviQI GTAC 4 cut(s) 10, 145, 327, 338
DdeI CTNAG 3 cut(s) 160, 705, 798
DpnI GATC 2 cut(s) 426, 507
DpnII GATC 2 cut(s) 424, 505
EaeI YGGCCR 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 212
Eco32I GATATC 1 cut(s) 817
Eco57I CTGAAG 2 cut(s) 477, 831
EcoO109I RGGNCCY 1 cut(s) 461
EcoRI GAATTC 2 cut(s) 587, 774
EcoRII CCWGG 3 cut(s) 186, 208, 553
EcoRV GATATC 1 cut(s) 817
FaeI CATG 3 cut(s) 245, 319, 412
FaiI YATR 9 cut(s) 150, 243, 255, 317, 380, 410, 438, 675, 852
FatI CATG 3 cut(s) 241, 315, 408
FblI GTMKAC 2 cut(s) 384, 747
Fnu4HI GCNGC 1 cut(s) 855
FokI GGATG 2 cut(s) 361, 707
Fsp4HI GCNGC 1 cut(s) 855
FspBI CTAG 1 cut(s) 111
FspI TGCGCA 1 cut(s) 654
GlaI GCGC 1 cut(s) 654
GluI GCNGC 1 cut(s) 855
GsaI CCCAGC 1 cut(s) 435
GsuI CTGGAG 2 cut(s) 236, 590
HaeIII GGCC 2 cut(s) 170, 462
HapII CCGG 2 cut(s) 174, 371
HgaI GACGC 1 cut(s) 733
HhaI GCGC 1 cut(s) 655
Hin1I GRCGYC 1 cut(s) 744
Hin1II CATG 3 cut(s) 245, 319, 412
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HinfI GANTC 5 cut(s) 56, 442, 493, 759, 794
HpaII CCGG 2 cut(s) 174, 371
HphI GGTGA 2 cut(s) 127, 385
Hpy166II GTNNAC 3 cut(s) 145, 385, 748
Hpy188I TCNGA 1 cut(s) 793
Hpy188III TCNNGA 1 cut(s) 520
Hpy8I GTNNAC 3 cut(s) 145, 385, 748
Hpy99I CGWCG 1 cut(s) 793
HpyAV CCTTC 2 cut(s) 85, 452
HpyCH4III ACNGT 4 cut(s) 44, 357, 422, 752
HpyCH4IV ACGT 2 cut(s) 12, 27
HpyCH4V TGCA 1 cut(s) 857
HpyF10VI GCNNNNNNNGC 2 cut(s) 312, 661
HpyF3I CTNAG 3 cut(s) 160, 705, 798
HpySE526I ACGT 2 cut(s) 12, 27
Hsp92I GRCGYC 1 cut(s) 744
Hsp92II CATG 3 cut(s) 245, 319, 412
HspAI GCGC 1 cut(s) 653
Kzo9I GATC 2 cut(s) 424, 505
LmnI GCTCC 2 cut(s) 263, 536
Lsp1109I GCAGC 1 cut(s) 841
LweI GCATC 1 cut(s) 607
MaeI CTAG 1 cut(s) 111
MaeII ACGT 2 cut(s) 12, 27
MaeIII GTNAC 2 cut(s) 44, 115
MalI GATC 2 cut(s) 426, 507
MboI GATC 2 cut(s) 424, 505
MboII GAAGA 4 cut(s) 83, 115, 577, 824
MhlI GDGCHC 1 cut(s) 604
MlsI TGGCCA 1 cut(s) 170
MluCI AATT 8 cut(s) 192, 307, 333, 342, 361, 525, 587, 774
MluNI TGGCCA 1 cut(s) 170
MlyI GAGTC 2 cut(s) 65, 487
MmeI TCCRAC 2 cut(s) 736, 771
MnlI CCTC 5 cut(s) 121, 377, 614, 685, 764
Mox20I TGGCCA 1 cut(s) 170
MscI TGGCCA 1 cut(s) 170
MseI TTAA 3 cut(s) 225, 543, 687
MslI CAYNNNNRTG 2 cut(s) 113, 246
Msp20I TGGCCA 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 455
MspI CCGG 2 cut(s) 174, 371
MspR9I CCNGG 4 cut(s) 188, 210, 371, 555
MvaI CCWGG 3 cut(s) 188, 210, 555
MwoI GCNNNNNNNGC 2 cut(s) 312, 661
NciI CCSGG 1 cut(s) 371
NdeII GATC 2 cut(s) 424, 505
NlaIII CATG 3 cut(s) 245, 319, 412
NlaIV GGNNCC 1 cut(s) 601
NmuCI GTSAC 2 cut(s) 44, 115
NsbI TGCGCA 1 cut(s) 654
OliI CACNNNNGTG 1 cut(s) 113
PfeI GAWTC 3 cut(s) 442, 759, 794
PkrI GCNGC 1 cut(s) 856
Ple19I CGATCG 1 cut(s) 508
PleI GAGTC 2 cut(s) 64, 487
PpsI GAGTC 2 cut(s) 64, 487
PsiI TTATAA 2 cut(s) 255, 675
Psp6I CCWGG 3 cut(s) 186, 208, 553
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 3 cut(s) 186, 208, 553
PspN4I GGNNCC 1 cut(s) 601
PspPI GGNCC 1 cut(s) 461
PvuI CGATCG 1 cut(s) 508
PvuII CAGCTG 1 cut(s) 455
RsaI GTAC 4 cut(s) 11, 146, 328, 339
RsaNI GTAC 4 cut(s) 10, 145, 327, 338
RseI CAYNNNNRTG 2 cut(s) 113, 246
SaqAI TTAA 3 cut(s) 225, 543, 687
SatI GCNGC 1 cut(s) 855
Sau3AI GATC 2 cut(s) 424, 505
Sau96I GGNCC 1 cut(s) 461
SchI GAGTC 2 cut(s) 65, 487
ScrFI CCNGG 4 cut(s) 188, 210, 371, 555
SduI GDGCHC 1 cut(s) 604
SfaNI GCATC 1 cut(s) 607
SfcI CTRYAG 1 cut(s) 418
SmiMI CAYNNNNRTG 2 cut(s) 113, 246
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
Sse9I AATT 8 cut(s) 192, 307, 333, 342, 361, 525, 587, 774
SsiI CCGC 2 cut(s) 152, 261
SspI AATATT 1 cut(s) 580
SspMI CTAG 1 cut(s) 111
StyD4I CCNGG 4 cut(s) 186, 208, 369, 553
TaaI ACNGT 4 cut(s) 44, 357, 422, 752
TaiI ACGT 2 cut(s) 15, 30
TaqI TCGA 3 cut(s) 504, 615, 643
TasI AATT 8 cut(s) 192, 307, 333, 342, 361, 525, 587, 774
TatI WGTACW 2 cut(s) 144, 326
TfiI GAWTC 3 cut(s) 442, 759, 794
Tru1I TTAA 3 cut(s) 225, 543, 687
Tru9I TTAA 3 cut(s) 225, 543, 687
TscAI CASTG 2 cut(s) 427, 731
TseFI GTSAC 2 cut(s) 44, 115
TseI GCWGC 1 cut(s) 854
Tsp45I GTSAC 2 cut(s) 44, 115
TspDTI ATGAA 4 cut(s) 230, 387, 434, 670
TspRI CASTG 2 cut(s) 427, 731
XapI RAATTY 4 cut(s) 361, 525, 587, 774
XmiI GTMKAC 2 cut(s) 384, 747
XspI CTAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.