MD15G1178700.v1.1

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008099
Physical Location & Seq
Reverse (-)
14063141 .. 14066114
2974 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1178700.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGAGCAGCACACAACTTTCAGACCAAATCTTCCGGCCATTAATCCCCGCAGCGTGTGTCAGTTTCCGGCAGCTGAGCACCACTAAGCCCAAACTACCGCCTTGCCCACGCCCTAAATCTTTGAAACACAAACACTTAACCCAAAAATCCAGGTGGGCTGTTAGGCTAAGCTTAGTGGACCAAAGCCCGCCAAAATCAACGTTTGACGTGGAACAGCTGGTAGGGTTTTTGTATGAGGATCTGGTTCATCTTTTTGATGATCGGGGAATTGATAGGACGGCGTACGATGAGCGTGTCAAGTTCAGGGACCCAATCACCAAGCATGACACAATAACTGGGTACTTGTTGAACATAGCCCTCTTGAAGATTGTGTTCACGCCTAAGTTCCAGTTGCACTGGGTCAAACAGGTAACAGGACCATGTGAAATCACTACAAGGTGGACTATGGTTATGAAGTTCATCCCTCTGCCTTGGAAACCAGAATTGATCTTCACAGGAACCTCTGTCATGGGTATCAACCCCGAGACTGGGAAGTTCTGCAGCCATGTGGACTTCTGGGATTCTATAAAGAATAATGAGTACTTTTCTTTAGAAGGTTTGTTGGATGTATTCAAGCAGCTGAGGATTTACAAAACTCCAGACTTGGAAACACCCAAATATGAAATACTAAAAAGAACTGCAAACTATGAGGTAAGAAAGTATGCACCTTATCTAGTGGTAGAAGGACCAGTAGACAAGCTGTCCGGGTCAGCTGGTTTCGGTGATGTTACCGGGTACATCTTTGGAAAGAACTCGAAAATGGAGAAGATACCGATGACCACTCCCGTCTTCACGGAGGCATCTGATGCTGAACTTTCCAAAGTATCCATCAAATTATGTCTTCCACTGGACAAGGACATAAGCAGCTTACCAGATCCCAATCAAGAAACAATTAGCTTGAAAAAAGTGGAAGGAGGCATTGCGGCCGTTGTCAAGTTCAGTGGAAAGCCTACCGAAGAGATTGTTCGGGAGAAGGAAAAAGTTTTGCGTGCTAGTCTGATCAGAGATGGTCTCAAACCAAAGGAGGGTTGTTCGCTTGCCCGTTACAACGATCCAGGCCGAACTTGGAGCTTTATGTTGAGGAATGAAGTACTGATAGGGCTGGAAGAGTTCACATTGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358 SOUL

Protein Analysis

388

Amino Acids

44.01

Weight (kDa)

8.89

Isoelectric Point (pI)

26.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 72 - 182 2.7e-26 Uncharacterized conserved protein (DUF2358)
SOUL PF04832 215 - 379 9.1e-37 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 732
AciI CCGC 4 cut(s) 48, 98, 188, 962
AclI AACGTT 1 cut(s) 200
AclWI GGATC 3 cut(s) 246, 908, 1085
AcoI YGGCCR 2 cut(s) 35, 963
AfaI GTAC 5 cut(s) 284, 341, 581, 776, 1131
AfiI CCNNNNNNNGG 3 cut(s) 527, 528, 1064
AgsI TTSAA 5 cut(s) 124, 349, 364, 613, 940
AhdI GACNNNNNGTC 1 cut(s) 739
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 2 cut(s) 149, 1093
AluBI AGCT 9 cut(s) 73, 171, 217, 619, 739, 752, 906, 936, 1110
AluI AGCT 9 cut(s) 73, 171, 217, 619, 739, 752, 906, 936, 1110
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 2 cut(s) 518, 1055
AlwI GGATC 3 cut(s) 246, 908, 1085
Ama87I CYCGRG 1 cut(s) 521
AoxI GGCC 3 cut(s) 35, 963, 1096
ApeKI GCWGC 6 cut(s) 6, 50, 70, 540, 616, 903
AseI ATTAAT 1 cut(s) 41
AspS9I GGNCC 4 cut(s) 178, 307, 416, 725
AsuC2I CCSGG 2 cut(s) 745, 772
AsuHPI GGTGA 2 cut(s) 307, 773
AvaI CYCGRG 1 cut(s) 521
AvaII GGWCC 4 cut(s) 178, 307, 416, 725
BbsI GAAGAC 2 cut(s) 820, 872
Bbv12I GWGCWC 1 cut(s) 80
BbvCI CCTCAGC 1 cut(s) 620
BbvI GCAGC 6 cut(s) 18, 62, 82, 552, 628, 915
BccI CCATC 2 cut(s) 875, 1040
BceAI ACGGC 2 cut(s) 294, 950
BciT130I CCWGG 2 cut(s) 151, 1095
BciVI GTATCC 1 cut(s) 874
BclI TGATCA 1 cut(s) 1038
BcnI CCSGG 2 cut(s) 745, 772
BcoDI GTCTC 2 cut(s) 518, 1055
BfaI CTAG 2 cut(s) 713, 1032
BfmI CTRYAG 1 cut(s) 538
BfuI GTATCC 1 cut(s) 874
BisI GCNGC 7 cut(s) 7, 51, 71, 541, 617, 904, 963
BlpI GCTNAGC 2 cut(s) 74, 167
BlsI GCNGC 7 cut(s) 8, 52, 72, 542, 618, 905, 964
BmcAI AGTACT 2 cut(s) 581, 1131
Bme1390I CCNGG 4 cut(s) 151, 745, 772, 1095
Bme18I GGWCC 4 cut(s) 178, 307, 416, 725
BmeRI GACNNNNNGTC 1 cut(s) 739
BmeT110I CYCGRG 1 cut(s) 521
BmgBI CACGTC 1 cut(s) 208
BmgT120I GGNCC 4 cut(s) 178, 307, 416, 725
BmiI GGNNCC 3 cut(s) 308, 309, 499
BmrFI CCNGG 4 cut(s) 151, 745, 772, 1095
BmrI ACTGGG 3 cut(s) 345, 406, 537
BmsI GCATC 2 cut(s) 835, 848
BmuI ACTGGG 3 cut(s) 345, 406, 537
BpiI GAAGAC 2 cut(s) 820, 872
BplI GAGNNNNNCTC 2 cut(s) 1035, 1067
BpmI CTGGAG 1 cut(s) 621
Bpu10I CCTNAGC 1 cut(s) 620
Bpu1102I GCTNAGC 2 cut(s) 74, 167
BpuMI CCSGG 2 cut(s) 745, 772
BsaBI GATNNNNATC 1 cut(s) 918
BsaI GGTCTC 1 cut(s) 1055
BsaJI CCNNGG 1 cut(s) 470
Bsc4I CCNNNNNNNGG 3 cut(s) 527, 528, 1064
Bse1I ACTGG 6 cut(s) 340, 388, 401, 532, 728, 891
Bse3DI GCAATG 1 cut(s) 957
Bse8I GATNNNNATC 1 cut(s) 918
BseBI CCWGG 2 cut(s) 151, 1095
BseDI CCNNGG 1 cut(s) 470
BseGI GGATG 2 cut(s) 459, 610
BseJI GATNNNNATC 1 cut(s) 918
BseLI CCNNNNNNNGG 3 cut(s) 527, 528, 1064
BseMI GCAATG 1 cut(s) 957
BseMII CTCAG 2 cut(s) 65, 611
BseNI ACTGG 6 cut(s) 340, 388, 401, 532, 728, 891
BseX3I CGGCCG 1 cut(s) 963
BseXI GCAGC 6 cut(s) 18, 62, 82, 552, 628, 915
Bsh1285I CGRYCG 1 cut(s) 966
BshFI GGCC 3 cut(s) 37, 965, 1098
BsiEI CGRYCG 1 cut(s) 966
BsiHKAI GWGCWC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 521
BsiSI CCGG 4 cut(s) 34, 67, 744, 771
BsiWI CGTACG 1 cut(s) 282
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 3 cut(s) 527, 528, 1064
BsmAI GTCTC 2 cut(s) 518, 1055
BsmFI GGGAC 1 cut(s) 320
BsnI GGCC 3 cut(s) 37, 965, 1098
Bso31I GGTCTC 1 cut(s) 1055
BsoBI CYCGRG 1 cut(s) 521
Bsp1286I GDGCHC 1 cut(s) 80
Bsp143I GATC 6 cut(s) 238, 259, 486, 913, 1038, 1090
Bsp1720I GCTNAGC 2 cut(s) 74, 167
BspACI CCGC 4 cut(s) 48, 98, 188, 962
BspANI GGCC 3 cut(s) 37, 965, 1098
BspCNI CTCAG 2 cut(s) 66, 612
BspLI GGNNCC 3 cut(s) 308, 309, 499
BspMAI CTGCAG 1 cut(s) 542
BspPI GGATC 3 cut(s) 246, 908, 1085
BspTNI GGTCTC 1 cut(s) 1055
BsrDI GCAATG 1 cut(s) 957
BsrI ACTGG 6 cut(s) 340, 388, 401, 532, 728, 891
BssECI CCNNGG 1 cut(s) 470
BssMI GATC 6 cut(s) 238, 259, 486, 913, 1038, 1090
BssT1I CCWWGG 1 cut(s) 470
Bst2UI CCWGG 2 cut(s) 151, 1095
Bst6I CTCTTC 2 cut(s) 990, 1140
BstAPI GCANNNNNTGC 1 cut(s) 845
BstC8I GCNNGC 3 cut(s) 188, 1029, 1077
BstDEI CTNAG 6 cut(s) 74, 84, 167, 172, 381, 620
BstF5I GGATG 2 cut(s) 459, 610
BstKTI GATC 6 cut(s) 241, 262, 489, 916, 1041, 1093
BstMAI GTCTC 2 cut(s) 518, 1055
BstMBI GATC 6 cut(s) 238, 259, 486, 913, 1038, 1090
BstMCI CGRYCG 1 cut(s) 966
BstMWI GCNNNNNNNGC 1 cut(s) 845
BstNI CCWGG 2 cut(s) 151, 1095
BstSCI CCNGG 4 cut(s) 149, 743, 770, 1093
BstSFI CTRYAG 1 cut(s) 538
BstV1I GCAGC 6 cut(s) 18, 62, 82, 552, 628, 915
BstV2I GAAGAC 2 cut(s) 820, 872
BstX2I RGATCY 2 cut(s) 238, 913
BstYI RGATCY 2 cut(s) 238, 913
BstZI CGGCCG 1 cut(s) 963
BsuI GTATCC 1 cut(s) 874
BsuRI GGCC 3 cut(s) 37, 965, 1098
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 2 cut(s) 459, 610
BtsIMutI CAGTG 3 cut(s) 394, 884, 985
Cac8I GCNNGC 3 cut(s) 188, 1029, 1077
Cfr13I GGNCC 4 cut(s) 178, 307, 416, 725
Csp6I GTAC 5 cut(s) 283, 340, 580, 775, 1130
CspCI CAANNNNNGTGG 2 cut(s) 961, 996
CviAII CATG 4 cut(s) 323, 420, 508, 545
CviQI GTAC 5 cut(s) 283, 340, 580, 775, 1130
DdeI CTNAG 6 cut(s) 74, 84, 167, 172, 381, 620
DpnI GATC 6 cut(s) 240, 261, 488, 915, 1040, 1092
DpnII GATC 6 cut(s) 238, 259, 486, 913, 1038, 1090
DriI GACNNNNNGTC 1 cut(s) 739
EaeI YGGCCR 2 cut(s) 35, 963
EagI CGGCCG 1 cut(s) 963
Eam1104I CTCTTC 2 cut(s) 990, 1140
Eam1105I GACNNNNNGTC 1 cut(s) 739
EarI CTCTTC 2 cut(s) 990, 1140
EclXI CGGCCG 1 cut(s) 963
Eco130I CCWWGG 1 cut(s) 470
Eco31I GGTCTC 1 cut(s) 1055
Eco47I GGWCC 4 cut(s) 178, 307, 416, 725
Eco52I CGGCCG 1 cut(s) 963
Eco88I CYCGRG 1 cut(s) 521
EcoO109I RGGNCCY 1 cut(s) 307
EcoRII CCWGG 2 cut(s) 149, 1093
EcoT14I CCWWGG 1 cut(s) 470
ErhI CCWWGG 1 cut(s) 470
FaeI CATG 4 cut(s) 326, 423, 511, 548
FaqI GGGAC 1 cut(s) 320
FatI CATG 4 cut(s) 322, 419, 507, 544
FauI CCCGC 2 cut(s) 55, 195
FbaI TGATCA 1 cut(s) 1038
FblI GTMKAC 1 cut(s) 732
Fnu4HI GCNGC 7 cut(s) 7, 51, 71, 541, 617, 904, 963
FokI GGATG 2 cut(s) 446, 617
Fsp4HI GCNGC 7 cut(s) 7, 51, 71, 541, 617, 904, 963
FspBI CTAG 2 cut(s) 713, 1032
GluI GCNGC 7 cut(s) 7, 51, 71, 541, 617, 904, 963
GsuI CTGGAG 1 cut(s) 621
HaeIII GGCC 3 cut(s) 37, 965, 1098
HapII CCGG 4 cut(s) 34, 67, 744, 771
Hin1II CATG 4 cut(s) 326, 423, 511, 548
HindIII AAGCTT 1 cut(s) 169
HinfI GANTC 1 cut(s) 560
HpaII CCGG 4 cut(s) 34, 67, 744, 771
HphI GGTGA 2 cut(s) 307, 773
Hpy166II GTNNAC 6 cut(s) 178, 375, 441, 550, 733, 1152
Hpy188I TCNGA 4 cut(s) 22, 844, 1038, 1043
Hpy188III TCNNGA 4 cut(s) 361, 638, 923, 1007
Hpy8I GTNNAC 6 cut(s) 178, 375, 441, 550, 733, 1152
HpyAV CCTTC 4 cut(s) 587, 716, 944, 1006
HpyCH4IV ACGT 2 cut(s) 200, 207
HpyCH4V TGCA 4 cut(s) 394, 540, 680, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 845
HpyF3I CTNAG 6 cut(s) 74, 84, 167, 172, 381, 620
HpySE526I ACGT 2 cut(s) 200, 207
Hsp92II CATG 4 cut(s) 326, 423, 511, 548
KflI GGGWCCC 1 cut(s) 307
Ksp22I TGATCA 1 cut(s) 1038
Kzo9I GATC 6 cut(s) 238, 259, 486, 913, 1038, 1090
LmnI GCTCC 1 cut(s) 1107
Lsp1109I GCAGC 6 cut(s) 18, 62, 82, 552, 628, 915
LweI GCATC 2 cut(s) 835, 848
MaeI CTAG 2 cut(s) 713, 1032
MaeII ACGT 2 cut(s) 200, 207
MaeIII GTNAC 3 cut(s) 409, 766, 1082
MalI GATC 6 cut(s) 240, 261, 488, 915, 1040, 1092
MboI GATC 6 cut(s) 238, 259, 486, 913, 1038, 1090
MboII GAAGA 8 cut(s) 22, 376, 481, 817, 820, 872, 1007, 1157
MflI RGATCY 2 cut(s) 238, 913
MhlI GDGCHC 1 cut(s) 80
MluCI AATT 4 cut(s) 267, 482, 872, 930
MmeI TCCRAC 1 cut(s) 582
MseI TTAA 3 cut(s) 41, 137, 1162
MspA1I CMGCKG 4 cut(s) 73, 217, 619, 752
MspI CCGG 4 cut(s) 34, 67, 744, 771
MspR9I CCNGG 4 cut(s) 151, 745, 772, 1095
MvaI CCWGG 2 cut(s) 151, 1095
MwoI GCNNNNNNNGC 1 cut(s) 845
NciI CCSGG 2 cut(s) 745, 772
NdeII GATC 6 cut(s) 238, 259, 486, 913, 1038, 1090
NlaIII CATG 4 cut(s) 326, 423, 511, 548
NlaIV GGNNCC 3 cut(s) 308, 309, 499
PfeI GAWTC 1 cut(s) 560
Pfl23II CGTACG 1 cut(s) 282
PkrI GCNGC 7 cut(s) 8, 52, 72, 542, 618, 905, 964
PpuMI RGGWCCY 1 cut(s) 307
PshBI ATTAAT 1 cut(s) 41
Psp1406I AACGTT 1 cut(s) 200
Psp5II RGGWCCY 1 cut(s) 307
Psp6I CCWGG 2 cut(s) 149, 1093
PspGI CCWGG 2 cut(s) 149, 1093
PspLI CGTACG 1 cut(s) 282
PspN4I GGNNCC 3 cut(s) 308, 309, 499
PspPI GGNCC 4 cut(s) 178, 307, 416, 725
PspPPI RGGWCCY 1 cut(s) 307
PstI CTGCAG 1 cut(s) 542
PsuI RGATCY 2 cut(s) 238, 913
PvuII CAGCTG 4 cut(s) 73, 217, 619, 752
RsaI GTAC 5 cut(s) 284, 341, 581, 776, 1131
RsaNI GTAC 5 cut(s) 283, 340, 580, 775, 1130
SaqAI TTAA 3 cut(s) 41, 137, 1162
SatI GCNGC 7 cut(s) 7, 51, 71, 541, 617, 904, 963
Sau3AI GATC 6 cut(s) 238, 259, 486, 913, 1038, 1090
Sau96I GGNCC 4 cut(s) 178, 307, 416, 725
ScaI AGTACT 2 cut(s) 581, 1131
ScrFI CCNGG 4 cut(s) 151, 745, 772, 1095
SduI GDGCHC 1 cut(s) 80
SfaNI GCATC 2 cut(s) 835, 848
SfcI CTRYAG 1 cut(s) 538
SinI GGWCC 4 cut(s) 178, 307, 416, 725
Sse9I AATT 4 cut(s) 267, 482, 872, 930
SsiI CCGC 4 cut(s) 48, 98, 188, 962
SspMI CTAG 2 cut(s) 713, 1032
StyD4I CCNGG 4 cut(s) 149, 743, 770, 1093
StyI CCWWGG 1 cut(s) 470
TaiI ACGT 2 cut(s) 203, 210
TaqI TCGA 1 cut(s) 794
TasI AATT 4 cut(s) 267, 482, 872, 930
TatI WGTACW 2 cut(s) 579, 1129
TauI GCSGC 1 cut(s) 965
TfiI GAWTC 1 cut(s) 560
Tru1I TTAA 3 cut(s) 41, 137, 1162
Tru9I TTAA 3 cut(s) 41, 137, 1162
TscAI CASTG 3 cut(s) 401, 891, 985
TseI GCWGC 6 cut(s) 6, 50, 70, 540, 616, 903
TspDTI ATGAA 5 cut(s) 236, 448, 467, 675, 1140
TspGWI ACGGA 1 cut(s) 848
TspRI CASTG 3 cut(s) 401, 891, 985
VpaK11BI GGWCC 4 cut(s) 178, 307, 416, 725
VspI ATTAAT 1 cut(s) 41
XcmI CCANNNNNNNNNTGG 1 cut(s) 1101
XmiI GTMKAC 1 cut(s) 732
XspI CTAG 2 cut(s) 713, 1032
ZrmI AGTACT 2 cut(s) 581, 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.