pycom15g16060

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008099
Physical Location & Seq
Reverse (-)
11179885 .. 11182317
2433 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g16060.1

Sequence Viewer

Length: 1092 bp
ATGAGCAGCACACAACTTTCAGACCAAATCTTCCGGCCATTAATCCCCGCCGCGTGTGTCAGTTTCCGGCAGATGAGCACCACTAAGCCCAAACTACTGCCCTGCCCACGCCCTAAATCTTTGAAACACAAACACTTAACGCAAAAATCCAAGTGGGCTGTTAGGCTAAGCTTAGTGGACCAAAGCCCGCCAAAATCAACGTTTGACGTGGAACAGCTAGTAGGGTTTTTGTATGAGGATCTGGTTCATCTTTTTGATGATCGGGGAATTGATAGGACGGCGTACGATGAGCGTGTCAAGTTCAGGGACCCAATCACCAAGCATGACACAATAGCTGGGTACTTGTTGAACATAGCCTTCTTGAAGATTGTGTTCACGCCTAGGTTCCAGTTGCACTGGGTCAAACAGACAGGACCATATGAAATCACTACAAGGTGGACTATGGTTATGAAGTTCATCCCTCTGCCTTGGAAACCAGAATTGATCTTCACAGGAACCTCTGTCATGGGCATCAACCCCGAGACTGGGAAGTTCTGCAGCCATGTGCTGAGGATTTACAAAACTCCAGACTTGCAAACACCCAAATATGAAATACTAAAAAGAACTGCAAACTATGAGGTAAGAAAGTATGCACCTTACGTAGTGGTAGAAGGACCAGTAGACAAGCTGTCCGGGTCAGCTGGTTTCGGTGATGTTACCGGGTACATCTTTGGAAAGAACTCGAAAATGGAGAAGATACCGATGACTACTCCCGTCTTCACCGAGGCATCTGATGCTGAACTTTCCAAAGTATCCATCAAATTATGTCTTCCACTGGACAAGGACATAAGCAGCTTACCAGATCCCAATCAAGAAACAATTAGCTTGAAAAAAGTGGAAGGAGGCATTGCTGCCGTTGTCAAGTTCAGTGGAAAGCCTACCGAAGATATTGTTCGGGAGAAGGAAAAAGCGTTGCGTGCTAGTCTGATCAGAGATGGTCTCAAACCAAAGGAGGGTTGTTCGCTTGCCCGTTACAATGATCCAGGCCGAACTTGGAGCTTTATGTTGAGGAATGAAGTACTGATAGGGCTGGAAGAGTTCACATTGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358 SOUL

Protein Analysis

364

Amino Acids

41.16

Weight (kDa)

9.25

Isoelectric Point (pI)

26.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 72 - 181 3.1e-27 Uncharacterized conserved protein (DUF2358)
SOUL PF04832 192 - 355 4.5e-37 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 660
AccII CGCG 1 cut(s) 53
AciI CCGC 3 cut(s) 48, 51, 188
AclI AACGTT 1 cut(s) 200
AclWI GGATC 3 cut(s) 246, 836, 1013
AcoI YGGCCR 1 cut(s) 35
AfaI GTAC 4 cut(s) 284, 341, 704, 1059
AfiI CCNNNNNNNGG 3 cut(s) 524, 525, 992
AgsI TTSAA 4 cut(s) 124, 349, 364, 868
AhdI GACNNNNNGTC 1 cut(s) 667
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 1 cut(s) 1021
AluBI AGCT 8 cut(s) 171, 217, 335, 667, 680, 834, 864, 1038
AluI AGCT 8 cut(s) 171, 217, 335, 667, 680, 834, 864, 1038
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 2 cut(s) 515, 983
AlwI GGATC 3 cut(s) 246, 836, 1013
Ama87I CYCGRG 1 cut(s) 518
AoxI GGCC 2 cut(s) 35, 1024
ApeKI GCWGC 4 cut(s) 6, 537, 831, 890
AseI ATTAAT 1 cut(s) 41
AspA2I CCTAGG 1 cut(s) 380
AspS9I GGNCC 4 cut(s) 178, 307, 413, 653
AsuC2I CCSGG 2 cut(s) 673, 700
AsuHPI GGTGA 3 cut(s) 307, 701, 751
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 4 cut(s) 178, 307, 413, 653
AvrII CCTAGG 1 cut(s) 380
BbsI GAAGAC 2 cut(s) 748, 800
Bbv12I GWGCWC 1 cut(s) 80
BbvCI CCTCAGC 1 cut(s) 548
BbvI GCAGC 4 cut(s) 18, 549, 843, 877
BccI CCATC 2 cut(s) 803, 968
BceAI ACGGC 2 cut(s) 294, 878
BciT130I CCWGG 1 cut(s) 1023
BciVI GTATCC 1 cut(s) 802
BclI TGATCA 1 cut(s) 966
BcnI CCSGG 2 cut(s) 673, 700
BcoDI GTCTC 2 cut(s) 515, 983
BfaI CTAG 3 cut(s) 218, 381, 960
BfmI CTRYAG 1 cut(s) 535
BfuI GTATCC 1 cut(s) 802
BisI GCNGC 5 cut(s) 7, 51, 538, 832, 891
BlnI CCTAGG 1 cut(s) 380
BlpI GCTNAGC 1 cut(s) 167
BlsI GCNGC 5 cut(s) 8, 52, 539, 833, 892
BmcAI AGTACT 1 cut(s) 1059
Bme1390I CCNGG 3 cut(s) 673, 700, 1023
Bme18I GGWCC 4 cut(s) 178, 307, 413, 653
BmeRI GACNNNNNGTC 1 cut(s) 667
BmeT110I CYCGRG 1 cut(s) 518
BmgBI CACGTC 1 cut(s) 208
BmgT120I GGNCC 4 cut(s) 178, 307, 413, 653
BmiI GGNNCC 4 cut(s) 308, 309, 386, 496
BmrFI CCNGG 3 cut(s) 673, 700, 1023
BmrI ACTGGG 2 cut(s) 406, 534
BmsI GCATC 3 cut(s) 519, 763, 776
BmuI ACTGGG 2 cut(s) 406, 534
BpiI GAAGAC 2 cut(s) 748, 800
BplI GAGNNNNNCTC 2 cut(s) 963, 995
BpmI CTGGAG 1 cut(s) 549
Bpu10I CCTNAGC 1 cut(s) 548
Bpu1102I GCTNAGC 1 cut(s) 167
BpuMI CCSGG 2 cut(s) 673, 700
BsaAI YACGTR 1 cut(s) 640
BsaBI GATNNNNATC 1 cut(s) 846
BsaI GGTCTC 1 cut(s) 983
BsaJI CCNNGG 3 cut(s) 380, 467, 762
Bsc4I CCNNNNNNNGG 3 cut(s) 524, 525, 992
Bse1I ACTGG 5 cut(s) 388, 401, 529, 656, 819
Bse3DI GCAATG 1 cut(s) 885
Bse8I GATNNNNATC 1 cut(s) 846
BseBI CCWGG 1 cut(s) 1023
BseDI CCNNGG 3 cut(s) 380, 467, 762
BseGI GGATG 1 cut(s) 456
BseJI GATNNNNATC 1 cut(s) 846
BseLI CCNNNNNNNGG 3 cut(s) 524, 525, 992
BseMI GCAATG 1 cut(s) 885
BseMII CTCAG 1 cut(s) 539
BseNI ACTGG 5 cut(s) 388, 401, 529, 656, 819
BseXI GCAGC 4 cut(s) 18, 549, 843, 877
BseYI CCCAGC 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 53
BshFI GGCC 2 cut(s) 37, 1026
BsiHKAI GWGCWC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 4 cut(s) 34, 67, 672, 699
BsiWI CGTACG 1 cut(s) 282
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 3 cut(s) 524, 525, 992
BsmAI GTCTC 2 cut(s) 515, 983
BsmFI GGGAC 1 cut(s) 320
BsnI GGCC 2 cut(s) 37, 1026
Bso31I GGTCTC 1 cut(s) 983
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 1 cut(s) 80
Bsp143I GATC 6 cut(s) 238, 259, 483, 841, 966, 1018
Bsp1720I GCTNAGC 1 cut(s) 167
BspACI CCGC 3 cut(s) 48, 51, 188
BspANI GGCC 2 cut(s) 37, 1026
BspCNI CTCAG 1 cut(s) 540
BspFNI CGCG 1 cut(s) 53
BspLI GGNNCC 4 cut(s) 308, 309, 386, 496
BspMAI CTGCAG 1 cut(s) 539
BspPI GGATC 3 cut(s) 246, 836, 1013
BspTNI GGTCTC 1 cut(s) 983
BsrDI GCAATG 1 cut(s) 885
BsrI ACTGG 5 cut(s) 388, 401, 529, 656, 819
BssECI CCNNGG 3 cut(s) 380, 467, 762
BssMI GATC 6 cut(s) 238, 259, 483, 841, 966, 1018
BssT1I CCWWGG 2 cut(s) 380, 467
Bst2UI CCWGG 1 cut(s) 1023
Bst6I CTCTTC 1 cut(s) 1068
BstAPI GCANNNNNTGC 1 cut(s) 773
BstBAI YACGTR 1 cut(s) 640
BstC8I GCNNGC 3 cut(s) 188, 957, 1005
BstDEI CTNAG 4 cut(s) 84, 167, 172, 548
BstF5I GGATG 1 cut(s) 456
BstFNI CGCG 1 cut(s) 53
BstKTI GATC 6 cut(s) 241, 262, 486, 844, 969, 1021
BstMAI GTCTC 2 cut(s) 515, 983
BstMBI GATC 6 cut(s) 238, 259, 483, 841, 966, 1018
BstMWI GCNNNNNNNGC 2 cut(s) 773, 956
BstNI CCWGG 1 cut(s) 1023
BstSCI CCNGG 3 cut(s) 671, 698, 1021
BstSFI CTRYAG 1 cut(s) 535
BstSNI TACGTA 1 cut(s) 640
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 4 cut(s) 18, 549, 843, 877
BstV2I GAAGAC 2 cut(s) 748, 800
BstX2I RGATCY 2 cut(s) 238, 841
BstYI RGATCY 2 cut(s) 238, 841
BsuI GTATCC 1 cut(s) 802
BsuRI GGCC 2 cut(s) 37, 1026
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 1 cut(s) 456
BtsIMutI CAGTG 3 cut(s) 394, 812, 913
Cac8I GCNNGC 3 cut(s) 188, 957, 1005
Cfr13I GGNCC 4 cut(s) 178, 307, 413, 653
Csp6I GTAC 4 cut(s) 283, 340, 703, 1058
CspCI CAANNNNNGTGG 2 cut(s) 889, 924
CviAII CATG 3 cut(s) 323, 505, 542
CviQI GTAC 4 cut(s) 283, 340, 703, 1058
DdeI CTNAG 4 cut(s) 84, 167, 172, 548
DpnI GATC 6 cut(s) 240, 261, 485, 843, 968, 1020
DpnII GATC 6 cut(s) 238, 259, 483, 841, 966, 1018
DriI GACNNNNNGTC 1 cut(s) 667
EaeI YGGCCR 1 cut(s) 35
Eam1104I CTCTTC 1 cut(s) 1068
Eam1105I GACNNNNNGTC 1 cut(s) 667
EarI CTCTTC 1 cut(s) 1068
Eco105I TACGTA 1 cut(s) 640
Eco130I CCWWGG 2 cut(s) 380, 467
Eco31I GGTCTC 1 cut(s) 983
Eco47I GGWCC 4 cut(s) 178, 307, 413, 653
Eco88I CYCGRG 1 cut(s) 518
EcoO109I RGGNCCY 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 1021
EcoT14I CCWWGG 2 cut(s) 380, 467
ErhI CCWWGG 2 cut(s) 380, 467
FaeI CATG 3 cut(s) 326, 508, 545
FaqI GGGAC 1 cut(s) 320
FatI CATG 3 cut(s) 322, 504, 541
FauI CCCGC 2 cut(s) 55, 195
FauNDI CATATG 1 cut(s) 418
FbaI TGATCA 1 cut(s) 966
FblI GTMKAC 1 cut(s) 660
Fnu4HI GCNGC 5 cut(s) 7, 51, 538, 832, 891
FokI GGATG 1 cut(s) 443
Fsp4HI GCNGC 5 cut(s) 7, 51, 538, 832, 891
FspBI CTAG 3 cut(s) 218, 381, 960
GluI GCNGC 5 cut(s) 7, 51, 538, 832, 891
GsaI CCCAGC 1 cut(s) 339
GsuI CTGGAG 1 cut(s) 549
HaeIII GGCC 2 cut(s) 37, 1026
HapII CCGG 4 cut(s) 34, 67, 672, 699
Hin1II CATG 3 cut(s) 326, 508, 545
HindIII AAGCTT 1 cut(s) 169
HpaII CCGG 4 cut(s) 34, 67, 672, 699
HphI GGTGA 3 cut(s) 307, 701, 751
Hpy166II GTNNAC 5 cut(s) 178, 375, 438, 661, 1080
Hpy188I TCNGA 4 cut(s) 22, 772, 966, 971
Hpy188III TCNNGA 4 cut(s) 361, 566, 851, 935
Hpy8I GTNNAC 5 cut(s) 178, 375, 438, 661, 1080
HpyAV CCTTC 4 cut(s) 367, 644, 872, 934
HpyCH4IV ACGT 3 cut(s) 200, 207, 639
HpyCH4V TGCA 5 cut(s) 394, 537, 574, 608, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 773, 956
HpyF3I CTNAG 4 cut(s) 84, 167, 172, 548
HpySE526I ACGT 3 cut(s) 200, 207, 639
Hsp92II CATG 3 cut(s) 326, 508, 545
KflI GGGWCCC 1 cut(s) 307
Ksp22I TGATCA 1 cut(s) 966
Kzo9I GATC 6 cut(s) 238, 259, 483, 841, 966, 1018
LmnI GCTCC 1 cut(s) 1035
Lsp1109I GCAGC 4 cut(s) 18, 549, 843, 877
LweI GCATC 3 cut(s) 519, 763, 776
MaeI CTAG 3 cut(s) 218, 381, 960
MaeII ACGT 3 cut(s) 200, 207, 639
MaeIII GTNAC 2 cut(s) 694, 1010
MalI GATC 6 cut(s) 240, 261, 485, 843, 968, 1020
MboI GATC 6 cut(s) 238, 259, 483, 841, 966, 1018
MboII GAAGA 8 cut(s) 22, 376, 478, 745, 748, 800, 935, 1085
MflI RGATCY 2 cut(s) 238, 841
MhlI GDGCHC 1 cut(s) 80
MluCI AATT 4 cut(s) 267, 479, 800, 858
MnlI CCTC 9 cut(s) 229, 471, 508, 543, 610, 757, 875, 985, 1041
MseI TTAA 2 cut(s) 41, 137
MspA1I CMGCKG 1 cut(s) 680
MspI CCGG 4 cut(s) 34, 67, 672, 699
MspR9I CCNGG 3 cut(s) 673, 700, 1023
MvaI CCWGG 1 cut(s) 1023
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 2 cut(s) 773, 956
NciI CCSGG 2 cut(s) 673, 700
NdeI CATATG 1 cut(s) 418
NdeII GATC 6 cut(s) 238, 259, 483, 841, 966, 1018
NlaIII CATG 3 cut(s) 326, 508, 545
NlaIV GGNNCC 4 cut(s) 308, 309, 386, 496
Pfl23II CGTACG 1 cut(s) 282
PkrI GCNGC 5 cut(s) 8, 52, 539, 833, 892
Ppu21I YACGTR 1 cut(s) 640
PpuMI RGGWCCY 1 cut(s) 307
PshBI ATTAAT 1 cut(s) 41
Psp1406I AACGTT 1 cut(s) 200
Psp5II RGGWCCY 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 1021
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 1 cut(s) 1021
PspLI CGTACG 1 cut(s) 282
PspN4I GGNNCC 4 cut(s) 308, 309, 386, 496
PspPI GGNCC 4 cut(s) 178, 307, 413, 653
PspPPI RGGWCCY 1 cut(s) 307
PstI CTGCAG 1 cut(s) 539
PsuI RGATCY 2 cut(s) 238, 841
PvuII CAGCTG 1 cut(s) 680
RsaI GTAC 4 cut(s) 284, 341, 704, 1059
RsaNI GTAC 4 cut(s) 283, 340, 703, 1058
SaqAI TTAA 2 cut(s) 41, 137
SatI GCNGC 5 cut(s) 7, 51, 538, 832, 891
Sau3AI GATC 6 cut(s) 238, 259, 483, 841, 966, 1018
Sau96I GGNCC 4 cut(s) 178, 307, 413, 653
ScaI AGTACT 1 cut(s) 1059
ScrFI CCNGG 3 cut(s) 673, 700, 1023
SduI GDGCHC 1 cut(s) 80
SfaNI GCATC 3 cut(s) 519, 763, 776
SfcI CTRYAG 1 cut(s) 535
SinI GGWCC 4 cut(s) 178, 307, 413, 653
SnaBI TACGTA 1 cut(s) 640
Sse9I AATT 4 cut(s) 267, 479, 800, 858
SsiI CCGC 3 cut(s) 48, 51, 188
SspMI CTAG 3 cut(s) 218, 381, 960
StyD4I CCNGG 3 cut(s) 671, 698, 1021
StyI CCWWGG 2 cut(s) 380, 467
TaiI ACGT 3 cut(s) 203, 210, 642
TaqI TCGA 1 cut(s) 722
TasI AATT 4 cut(s) 267, 479, 800, 858
TatI WGTACW 1 cut(s) 1057
TauI GCSGC 1 cut(s) 53
Tru1I TTAA 2 cut(s) 41, 137
Tru9I TTAA 2 cut(s) 41, 137
TscAI CASTG 3 cut(s) 401, 819, 913
TseI GCWGC 4 cut(s) 6, 537, 831, 890
TspDTI ATGAA 6 cut(s) 236, 435, 445, 464, 603, 1068
TspRI CASTG 3 cut(s) 401, 819, 913
VpaK11BI GGWCC 4 cut(s) 178, 307, 413, 653
VspI ATTAAT 1 cut(s) 41
XcmI CCANNNNNNNNNTGG 1 cut(s) 1029
XmaJI CCTAGG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 660
XspI CTAG 3 cut(s) 218, 381, 960
ZrmI AGTACT 1 cut(s) 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.